{"id":1437,"date":"2018-09-30T00:00:00","date_gmt":"2018-09-29T15:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/user_voice_42_3_stemflex\/"},"modified":"2023-12-28T14:09:36","modified_gmt":"2023-12-28T05:09:36","slug":"user_voice_42_3_stemflex","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/user_voice_42_3_stemflex\/","title":{"rendered":"\u795e\u7d4c\u3084\u7b4b\u3082\u5099\u3048\u305f\u6a5f\u80fd\u7684\u7acb\u4f53\u81d3\u5668\u300c\u30df\u30cb\u8178\u300d\u3092\u5275\u308a\u51fa\u3059\uff08\u56fd\u7acb\u6210\u80b2\u533b\u7642\u7814\u7a76\u30bb\u30f3\u30bf\u30fc\u7814\u7a76\u6240 \u963f\u4e45\u6d25\u82f1\u61b2 \u6c0f\uff09-\u300cNEXT\u300d2017\u5e7410\u6708\u53f7\u63b2\u8f09"},"content":{"rendered":"<h2><span style=\"font-size: 12pt\"><strong>\u9375\u306f\u591a\u80fd\u6027\u5e79\u7d30\u80de\u304c\u6709\u3059\u308b\u81ea\u5df1\u7d44\u7e54\u5316\u3092\u3069\u3046\u5f15\u304d\u3060\u3059\u304b<\/strong><\/span><\/h2>\n<p><strong>\u963f\u4e45\u6d25\u82f1\u61b2 \u6c0f\uff08 \u56fd\u7acb\u6210\u80b2\u533b\u7642\u7814\u7a76\u30bb\u30f3\u30bf\u30fc\u7814\u7a76\u6240\u518d\u751f\u533b\u7642\u30bb\u30f3\u30bf\u30fc\u751f\u6b96\u533b\u7642\u7814\u7a76\u90e8\u90e8\u9577\uff09<\/strong><\/p>\n<p><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/66c19942ab4ba346fdb64ccc04cde373-4-1.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignleft size-full wp-image-4499\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/66c19942ab4ba346fdb64ccc04cde373-4-1.jpg\" alt=\"\u963f\u4e45\u6d25\u82f1\u61b2 \u6c0f\uff08 \u56fd\u7acb\u6210\u80b2\u533b\u7642\u7814\u7a76\u30bb\u30f3\u30bf\u30fc\u7814\u7a76\u6240\u518d\u751f\u533b\u7642\u30bb\u30f3\u30bf\u30fc\u751f\u6b96\u533b\u7642\u7814\u7a76\u90e8\u90e8\u9577\uff09\" width=\"250\" height=\"175\" \/><\/a>\u5c06\u6765\u7684\u306a\u533b\u7642\u5fdc\u7528\u3092\u898b\u636e\u3048\u3001\u7570\u7a2e\u52d5\u7269\u7531\u6765\u6210\u5206\u3092\u542b\u307e\u306a\u3044\u30bc\u30ce\u30d5\u30ea\u30fc\u306e\u57f9\u990a\u6761\u4ef6\u306b\u3053\u3060\u308f\u308a\u3001\u3053\u308c\u307e\u3067\u306b\u3044\u304f\u3064\u3082\u306e\u30d2\u30c8ES\u7d30\u80de\u3092\u6a39\u7acb\u3057\u3066\u304d\u305f\u56fd\u7acb\u6210\u80b2\u533b\u7642\u7814\u7a76\u30bb\u30f3\u30bf\u30fc\u306e\u963f\u4e45\u6d25\u82f1\u61b2\u6c0f\u30022017\u5e741\u6708\u3001ES\u7d30\u80de\u304b\u3089\u6a5f\u80fd\u3059\u308b\u8178\u7ba1\u30aa\u30eb\u30ac\u30ce\u30a4\u30c9\u300c\u30df\u30cb\u8178\u300d\u3092\u5275\u308a\u51fa\u3059\u3053\u3068\u306b\u6210\u529f\u3057\u3001\u5831\u544a\u3057\u307e\u3057\u305f\u300c\u751f\u4f53\u5185\u3068\u540c\u3058\u3088\u3046\u306b\u3001\u8907\u6570\u306e\u7a2e\u985e\u306e\u7d30\u80de\u304c\u9023\u643a\u3057\u3066\u50cd\u304f\u3001\u7acb\u4f53\u7684\u3067\u6a5f\u80fd\u7684\u306a\u81d3\u5668\u3092\u5275\u308a\u305f\u3044\u300d\u3068\u3044\u3046\u3001\u963f\u4e45\u6d25\u6c0f\u306e\u5f37\u3044\u4fe1\u5ff5\u304c\u4eca\u56de\u306e\u6210\u679c\u306b\u3064\u306a\u304c\u3063\u3066\u3044\u307e\u3059\u3002\u300c\u30df\u30cb\u8178\u300d\u306f\u3001\u751f\u4f53\u306e\u8178\u3068\u540c\u3058\u3088\u3046\u306b\u5438\u53ce\u3084\u6392\u51fa\u3084\u8815\u52d5\u904b\u52d5\u3092\u884c\u3046\u3060\u3051\u3067\u306a\u304f\u3001\u85ac\u5264\u3078\u306e\u5fdc\u7b54\u3082\u30d2\u30c8\u8178\u3068\u8fd1\u4f3c\u3057\u3066\u3044\u307e\u3059\u3002\u963f\u4e45\u6d25\u6c0f\u306b\u3001\u300c\u30df\u30cb\u8178\u300d\u5275\u751f\u306e\u6982\u8981\u3068\u610f\u7fa9\u3001\u305d\u3057\u3066\u4eca\u5f8c\u306e\u5c55\u958b\u306b\u3064\u3044\u3066\u4f3a\u3044\u307e\u3057\u305f\u3002<br \/>\n<span style=\"font-size: 8pt\">\u203bA xenogeneic-free system generating functional human gut organ oids from pluripotent stem cells. 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loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-4401 alignnone\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/P10_fig2.jpg\" alt=\"\u30df\u30cb\u8178\" width=\"250\" height=\"175\" \/><span style=\"font-size: 9pt\"><strong>[\u30df\u30cb\u8178\u306f\u5927\u304d\u3055\u304c1-2cm\u3067\u3001\u8178\u7ba1\u8815\u52d5\u69d8\u904b\u52d5\u3082\u8a8d\u3081\u3089\u308c\u307e\u3059\u3002]<\/strong><\/span><\/p>\n<h2><span style=\"font-size: 12pt\">\u74b0\u5883\u3092\u6574\u3048\u3066\u5e79\u7d30\u80de\u306e\u80cc\u4e2d\u3092\u62bc\u3059<\/span><\/h2>\n<p>\u6b21\u306b\u30df\u30cb\u8178\u306e\u4f5c\u88fd\u306b\u3042\u305f\u308a\u91cd\u8996\u3057\u305f\u70b9\u3092\u304a\u805e\u304d\u3057\u307e\u3057\u305f\u3002\u300c\u30b7\u30f3\u30d7\u30eb\u306a\u65b9\u6cd5\u3067\u81ea\u5df1\u7d44\u7e54\u5316\u80fd\u3092\u5f15\u304d\u51fa\u3059\u305f\u3081\u306b\u306f\u3001\u57f9\u990a\u30c7\u30a3\u30c3\u30b7\u30e5\u3068\u5171\u306b\u57f9\u5730\u306e\u9078\u629e\u3082\u5927\u5207\u3067\u3059\u3002\u533b\u7642\u3078\u306e\u5fdc\u7528\u304c\u524d\u63d0\u306a\u306e\u3067\u30bc\u30ce\u30d5\u30ea\u30fc\u74b0\u5883\u306f\u8b72\u308c\u307e\u305b\u3093\u3002\u3053\u306e\u7814\u7a76\u3067\u306f\u3001Gibco\u2122 Knock out\u2122 DMEM\u3068Gibco\u2122 CTS \u2122 Knock Out\u2122 SR XenoFreemedium\u3092\u30d9\u30fc\u30b9\u306b\u30aa\u30ea\u30b8\u30ca\u30eb\u57f9\u5730\u3092\u4f7f\u7528\u3057\u307e\u3057\u305f\u3002\u30d2\u30c8ES\u7d30\u80de\u306e\u6a39\u7acb\u306e\u6642\u304b\u3089\u4f7f\u3044\u7d9a\u3051\u3066\u304d\u305f\u57f9\u5730\u30b7\u30ea\u30fc\u30ba\u3067\u3042\u308a\u3001\u30ed\u30c3\u30c8\u9593\u5dee\u306e\u5c11\u306a\u3055\u3001\u6838\u578b\u306e\u7dad\u6301\u3084\u5897\u6b96\u80fd\u306a\u3069\u306e\u89b3\u70b9\u304b\u3089\u3001\u4ed6\u306e\u57f9\u5730\u7b49\u3068\u6bd4\u8f03\u691c\u8a0e\u3057\u307e\u3057\u305f\u3002\u307e\u305f\u65b0\u3057\u3044Gibco\u2122StemFlex\u2122 Medium\u306f\u3001\u591a\u80fd\u6027\u5e79\u7d30\u80de\u306e\u5897\u6b96\u80fd\u3092\u9ad8\u304b\u3081\u3001\u672a\u5206\u5316\u80fd\u306e\u7dad\u6301\u306b\u512a\u308c\u3066\u3044\u308b\u306e\u3067\u3001\u5b89\u5b9a\u306a\u5e79\u7d30\u80de\u57f9\u990a\u306b\u9069\u3057\u3066\u3044\u308b\u69d8\u3067\u3059\u3002\u4eca\u5f8c\u3001\u3055\u3089\u306b\u691c\u8a3c\u3057\u3066\u3044\u304d\u305f\u3044\u3067\u3059\u306d\u3002\u79c1\u305f\u3061\u306e\u7814\u7a76\u306f\u3001\u57f9\u990a\u6761\u4ef6\u3092\u6d17\u7df4\u3059\u308b\u3053\u3068\u3067\u81ea\u5df1\u7d44\u7e54\u5316\u80fd\u3092\u5f15\u304d\u51fa\u3059\u3001\u3044\u308f\u3070\u5e79\u7d30\u80de\u306e\u80cc\u4e2d\u3092\u62bc\u3059\u30a4\u30e1\u30fc\u30b8\u3067\u5206\u5316\u7814\u7a76\u3092\u9032\u3081\u3066\u3044\u307e\u3059\u3002\u57f9\u5730\u306e\u8cea\u3084\u30c7\u30a3\u30c3\u30b7\u30e5\u306a\u3069\u306e\u57f9\u990a\u74b0\u5883\u306e\u6700\u9069\u5316\u306f\u975e\u5e38\u306b\u91cd\u8981\u300d\u3068\u963f\u4e45\u6d25\u6c0f\u306f\u8a71\u3057\u307e\u3059\u3002\u305d\u3057\u3066\u4eca\u56de\u306e\u57f9\u990a\u6cd5\u306e\u30b3\u30f3\u30bb\u30d7\u30c8\u3092\u3001\u300c\u30bc\u30ce\u30d5\u30ea\u30fc\u3067\u3042\u308b\u3053\u3068\u3001\u7c21\u5358\u3067\u3042\u308b\u3053\u3068\u3001\u305d\u3057\u3066\u6a5f\u80fd\u6027\u3092\u5099\u3048\u3066\u3044\u308b\u3053\u3068\u306e3\u70b9\u3060\u300d\u3068\u5f37\u8abf\u3057\u307e\u3059\u3002<\/p>\n<h2><span style=\"font-size: 12pt\">\u300c\u30df\u30cb\u8178\u300d\u5275\u751f\u306e\u610f\u7fa9\u3001\u305d\u3057\u3066\u4eca\u5f8c\u306e\u5c55\u958b<\/span><\/h2>\n<p><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/84I5043_0911.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignleft size-full wp-image-4256\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/84I5043_0911.jpg\" alt=\"\u963f\u4e45\u6d25\u82f1\u61b2 \u6c0f\uff08 \u56fd\u7acb\u6210\u80b2\u533b\u7642\u7814\u7a76\u30bb\u30f3\u30bf\u30fc\u7814\u7a76\u6240\u518d\u751f\u533b\u7642\u30bb\u30f3\u30bf\u30fc\u751f\u6b96\u533b\u7642\u7814\u7a76\u90e8\u90e8\u9577\uff09\" width=\"250\" height=\"166\" \/><\/a>\u8178\u7ba1\u306f\u7c98\u819c\u4e0a\u76ae\u3084\u7c98\u819c\u4e0b\u7d44\u7e54\u3001\u8178\u7ba1\u795e\u7d4c\u3068\u3053\u308c\u3089\u3092\u53d6\u308a\u5dfb\u304f\u7b4b\u8089\u306a\u3069\u3001\u4e09\u80da\u8449\u306b\u7531\u6765\u3059\u308b\u591a\u5f69\u306a\u7d30\u80de\u3067\u69cb\u6210\u3055\u308c\u308b\u8907\u96d1\u306a\u7d44\u7e54\u3067\u3001\u3053\u308c\u307e\u3067\u5206\u5316\u8a98\u5c0e\u306f\u56f0\u96e3\u3068\u3055\u308c\u3066\u304d\u307e\u3057\u305f\u3002\u963f\u4e45\u6d25\u6c0f\u304c\u767a\u8868\u3057\u305f\u30df\u30cb\u8178\u306f\u7d44\u7e54\u69cb\u9020\u3092\u5fe0\u5b9f\u306b\u518d\u73fe\u3057\u3066\u304a\u308a\u3001\u521d\u3081\u3066\u306e\u7acb\u4f53\u8178\u7ba1\u30e2\u30c7\u30eb\u3068\u3057\u3066\u3001\u85ac\u5264\u5fdc\u7b54\u306e\u89e3\u6790\u3084\u96e3\u6cbb\u6027\u8178\u75be\u60a3\u306e\u75c5\u7406\u89e3\u660e\u3001\u6cbb\u7642\u6cd5\u958b\u767a\u306b\u6d3b\u304b\u3055\u308c\u308b\u3068\u671f\u5f85\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u300c\u65e5\u672c\u3067\u306f\u6f70\u760d\u6027\u5927\u8178\u708e\u3084\u30af\u30ed\u30fc\u30f3\u75c5\u306a\u3069\u91cd\u7be4\u306a\u8178\u306e\u75be\u60a3\u304c\u5897\u52a0\u50be\u5411\u3067\u3001\u75c5\u614b\u89e3\u660e\u3001\u65b0\u85ac\u958b\u767a\u306e\u305f\u3081\u306b\u306f<i>in vitro<\/i>\u3067\u89e3\u6790\u3067\u304d\u308b\u30d0\u30a4\u30aa\u30c4\u30fc\u30eb\u304c\u5fc5\u8981\u3067\u3057\u305f\u300d\u3068\u963f\u4e45\u6d25\u6c0f\u306f\u6307\u6458\u3057\u307e\u3059\u3002\u963f\u4e45\u6d25\u6c0f\u304c\u6240\u5c5e\u3059\u308b\u6210\u80b2\u533b\u7642\u7814\u7a76\u30bb\u30f3\u30bf\u30fc\u306b\u306f\u3001\u65b0\u751f\u5150\u671f\u304b\u3089\u907a\u4f1d\u6027\u3067\u767a\u75c5\u3059\u308b\u91cd\u3044\u8178\u75be\u60a3\u3067\u5165\u9662\u3059\u308b\u5b50\u4f9b\u3082\u591a\u3044\u305d\u3046\u3067\u3059\u3002\u300c\u79c1\u306e\u7814\u7a76\u306e\u539f\u70b9\u306f\u3001\u96e3\u75c5\u306e\u5b50\u4f9b\u305f\u3061\u3092\u6551\u3046\u3053\u3068\u3002\u5e38\u306b\u3053\u306e\u30bb\u30f3\u30bf\u30fc\u306e\u7406\u5ff5\u3092\u610f\u8b58\u3057\u3066\u7814\u7a76\u306b\u53d6\u308a\u7d44\u3093\u3067\u3044\u307e\u3059\u300d\u3002\u85ac\u5264\u306e\u958b\u767a\u3084\u6cbb\u7642\u6cd5\u958b\u767a\u306b\u5fc5\u8981\u306a\u81d3\u5668\u30aa\u30eb\u30ac\u30ce\u30a4\u30c9\u3092\u30b7\u30f3\u30d7\u30eb\u306b\u5275\u308a\u51fa\u3059\u3001\u963f\u4e45\u6d25\u6c0f\u306f\u7cbe\u529b\u7684\u306b\u7814\u7a76\u3092\u7d9a\u3051\u3066\u3044\u307e\u3059\u3002<\/p>\n<h2><span style=\"font-size: 12pt\">\u30b2\u30ce\u30e0\u7de8\u96c6\u3084\u30b7\u30f3\u30b0\u30eb\u30bb\u30eb\u7d99\u4ee3\u306a\u3069\u306b\u9069\u3057\u305f\u591a\u80fd\u6027\u5e79\u7d30\u80de\u7528\u57f9\u5730<\/span><br \/>\n<span style=\"font-size: 12pt\">StemFlex Medium<\/span><\/h2>\n<p><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-content\/uploads\/2018\/09\/temp.jpg\">\u00a0<\/a><a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/stem-cell-research\/induced-pluripotent-stem-cells\/medium-robust-feeder-free-psc-culture\/?CID=bid_clb_scl_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_stem2n_Social_LAB\" target=\"_blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" class=\"alignleft size-full wp-image-4258\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/COL04801-StemFlex-basal-ver2-W.jpg\" alt=\"\u963f\u4e45\u6d25\u82f1\u61b2 \u6c0f\uff08 \u56fd\u7acb\u6210\u80b2\u533b\u7642\u7814\u7a76\u30bb\u30f3\u30bf\u30fc\u7814\u7a76\u6240\u518d\u751f\u533b\u7642\u30bb\u30f3\u30bf\u30fc\u751f\u6b96\u533b\u7642\u7814\u7a76\u90e8\u90e8\u9577\uff09\" width=\"250\" height=\"201\" \/><\/a>\u6700\u65b0\u306e\u5e79\u7d30\u80de\u7814\u7a76\u306b\u5fc5\u8981\u3068\u3055\u308c\u308b\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306b\u304a\u3044\u3066\u3082\u3001\u512a\u308c\u305f\u6027\u80fd\u3092\u767a\u63ee\u3059\u308b\u30d5\u30a3\u30fc\u30c0\u30fc\u30d5\u30ea\u30fc\u3067\u884c\u3046\u591a\u80fd\u6027\u5e79\u7d30\u80de\u7528\u57f9\u5730\u3067\u3059\u3002\u5897\u6b96\u80fd\u304c\u5411\u4e0a\u3057\u3001\u69d8\u3005\u306a\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306b\u67d4\u8edf\u306b\u5bfe\u5fdc\u3057\u307e\u3059\u3002\u5b9f\u9a13\u7528\u9014\u306b\u5fdc\u3058\u3066\u3001Gibco\u2122 Geltrex\u2122 \u3084Gibco\u2122 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