{"id":14385,"date":"2023-06-08T18:02:06","date_gmt":"2023-06-08T09:02:06","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/?p=14385"},"modified":"2024-09-18T14:10:01","modified_gmt":"2024-09-18T05:10:01","slug":"gene-editing_bid_ts_6","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/gene-editing_bid_ts_6\/","title":{"rendered":"\u305d\u3046\u3044\u3046\u3053\u3068\u3060\u3063\u305f\u306e\u304b ! \u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\uff08CRISPR\/Cas9\uff09 \uff5e\u7b2c6\u56de\u3000\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u52b9\u679c\uff5e"},"content":{"rendered":"<p>\u30b2\u30ce\u30e0\u7de8\u96c6\u306e\u521d\u5fc3\u8005A\u541b\u306b\u3088\u308b\u300c\u521d\u3081\u3066\u306e\u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\u300d\u3001<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/gene-editing_bid_ts_5\/?CID=bid_syn_gns_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_lgn_kt_s00_gene_editing_bid_ts_6_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u524d\u56de<\/a>\u306f\u3001\u5909\u7570\u5c0e\u5165\u52b9\u7387\u3068\u5909\u7570\u306e\u78ba\u8a8d\u65b9\u6cd5\u306b\u3064\u3044\u3066\u5b66\u3073\u307e\u3057\u305f\u3002<br \/>\n<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-14304\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/05\/29d3bc99193cb0b837fb8cc53f050ae5-2.png\" alt=\"\" width=\"524\" height=\"283\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/05\/29d3bc99193cb0b837fb8cc53f050ae5-2.png 524w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/05\/29d3bc99193cb0b837fb8cc53f050ae5-2-300x162.png 300w\" sizes=\"auto, (max-width: 524px) 100vw, 524px\" \/><br \/>\n\u524d\u56de\u3054\u7d39\u4ecb\u3057\u305f\u6d41\u308c\u306b\u5f93\u3063\u3066\u3001\u30b2\u30ce\u30e0\u7de8\u96c6\u3067\u5909\u7570\u304c\u5165\u3063\u3066\u3044\u308b\u3053\u3068\u304c\u78ba\u8a8d\u3067\u304d\u308c\u3070\u3001\u3072\u3068\u307e\u305a\u306f\u5b89\u5fc3\u3067\u3059\u3002\u3057\u304b\u3057\u3001\u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\u3092\u3053\u3053\u3067\u7d42\u308f\u3089\u305b\u3066\u306f\u3044\u3051\u307e\u305b\u3093\u3002\u3082\u3046\u3072\u3068\u3064\u3084\u308b\u3053\u3068\u304c\u6b8b\u3063\u3066\u3044\u307e\u3059\u3002\u3069\u3093\u306a\u306b\u512a\u308c\u305f\u30c7\u30b6\u30a4\u30f3\u306e\u30ac\u30a4\u30c9RNA\u3067\u3082\u907f\u3051\u3066\u306f\u901a\u308c\u306a\u3044<b>\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u52b9\u679c<\/b>\u3092\u78ba\u8a8d\u3059\u308b\u5fc5\u8981\u304c\u3042\u308b\u305f\u3081\u3067\u3059\u3002<\/p>\n<p>\u7b2c6\u56de\u3067\u306f\u3001\u3053\u306e\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u52b9\u679c\u306b\u3064\u3044\u3066\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n<h2>\u30b2\u30ce\u30e0\u7de8\u96c6\u306e\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u52b9\u679c\u3068\u306f\uff1f<\/h2>\n<p><span style=\"color: #3366ff\">A\u3055\u3093<\/span>\uff1a<br \/>\n\u5148\u8f29\u3002\u5b9f\u306f\u30bf\u30fc\u30b2\u30c3\u30c8\u907a\u4f1d\u5b50\u3068\u914d\u5217\u304c\u4f3c\u3066\u3044\u308b\u5225\u306e\u907a\u4f1d\u5b50\u306e\u914d\u5217\u3092\u898b\u3066\u307f\u305f\u3093\u3067\u3059\u304c\u3001\u3053\u3053\u306b\u3082\u5909\u7570\u304c\u5165\u3063\u3066\u3044\u308b\u304b\u3082\u77e5\u308c\u307e\u305b\u3093\u3002\u3069\u3046\u3057\u307e\u3057\u3087\u3046\u3002<\/p>\n<p><span style=\"color: #ff00ff\">\u5148\u8f29<\/span>\uff1a<br \/>\n\u305d\u308c\u306f\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u52b9\u679c\u3060\u306d\u3002\u30b2\u30ce\u30e0\u7de8\u96c6\u3067\u306f\u591a\u304b\u308c\u5c11\u306a\u304b\u308c\u8d77\u3053\u308b\u3053\u3068\u3060\u3088\u3002<\/p>\n<p><span style=\"color: #3366ff\">A\u3055\u3093<\/span>\uff1a<br \/>\n\u305d\u3046\u306a\u3093\u3067\u3059\u304b\uff1f\u306a\u3093\u3060\u3001\u5fc3\u914d\u3057\u3061\u3083\u3044\u307e\u3057\u305f\u3002<\/p>\n<p><span style=\"color: #ff00ff\">\u5148\u8f29<\/span>\uff1a<br \/>\n\u3069\u3093\u306a\u306b\u30ac\u30a4\u30c9RNA\u306e\u30c7\u30b6\u30a4\u30f3\u304c\u512a\u308c\u3066\u3044\u3066\u3082\u3001\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u52b9\u679c\u306f\u8d77\u304d\u308b\u306e\u304c\u666e\u901a\u3060\u3088\u3002\u3060\u304b\u3089\u3001\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u53ef\u80fd\u6027\u306e\u3042\u308b\u914d\u5217\u4ed8\u8fd1\u3082\u3001\u30b7\u30fc\u30b1\u30f3\u30b9\u3067\u3057\u3063\u304b\u308a\u898b\u3066\u304a\u304f\u3053\u3068\u304c\u3068\u3066\u3082\u5927\u5207\u306a\u3093\u3060\u3002<\/p>\n<p>\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u52b9\u679c\u3068\u306f\u3001\u76ee\u7684\u306e\u907a\u4f1d\u5b50\u4ee5\u5916\u306e\u3068\u3053\u308d\u306b\u5909\u7570\u304c\u5165\u308b\u3053\u3068\u3092\u6307\u3057\u307e\u3059\u3002\u3069\u308c\u3060\u3051\u30ac\u30a4\u30c9RNA\u306e\u30c7\u30b6\u30a4\u30f3\u3092\u5de5\u592b\u3057\u3066\u3082\u3001\u5bfe\u7b56\u3092\u7acb\u3066\u3066\u3082\u3001\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u304c\u30bc\u30ed\u306b\u306a\u308b\u3053\u3068\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u305d\u306e\u305f\u3081\u3001\u300c\u57fa\u672c\u7684\u306b\u306f\u8d77\u3053\u308b\u53ef\u80fd\u6027\u306e\u3042\u308b\u3082\u306e\u300d\u3068\u3057\u3066\u3001\u30aa\u30f3\u30bf\u30fc\u30b2\u30c3\u30c8\uff08\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u5909\u7570\uff09\u3092\u30b7\u30fc\u30b1\u30f3\u30b9\u3067\u78ba\u8a8d\u3059\u308b\u3068\u304d\u3001\u4f75\u305b\u3066\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u6709\u7121\u3082\u78ba\u8a8d\u3057\u307e\u3057\u3087\u3046\u3002<\/p>\n<p>\u3067\u306f\u3001\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u3092\u3069\u306e\u3088\u3046\u306b\u3057\u3066\u78ba\u8a8d\u3059\u308c\u3070\u3088\u3044\u3067\u3057\u3087\u3046\u304b\u3002<\/p>\n<p>\u3082\u3061\u308d\u3093\u5168\u30b2\u30ce\u30e0\u914d\u5217\u3092\u78ba\u8a8d\u3059\u308c\u3070\u9593\u9055\u3044\u306a\u3044\u3067\u3059\u304c\u3001\u6b21\u4e16\u4ee3\u30b7\u30fc\u30b1\u30f3\u30b5\u306a\u3069\u5927\u578b\u6a5f\u5668\u304c\u5fc5\u8981\u306b\u306a\u3063\u3066\u3057\u307e\u3044\u3001\u89e3\u6790\u3082\u5927\u5909\u306b\u306a\u3063\u3066\u3057\u307e\u3044\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u3001<b>\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u3092\u5f15\u304d\u8d77\u3053\u3059\u914d\u5217\u4ed8\u8fd1\u306e\u307f\u3092\u30b7\u30fc\u30b1\u30f3\u30b9\u3067\u78ba\u8a8d\u3057\u307e\u3057\u3087\u3046\u3002<\/b><\/p>\n<p>\u3067\u306f\u3001\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u3092\u5f15\u304d\u8d77\u3053\u3059\u914d\u5217\u306f\u3069\u306e\u3088\u3046\u306b\u7279\u5b9a\u3059\u308c\u3070\u3088\u3044\u3067\u3057\u3087\u3046\u304b\u3002<\/p>\n<p>\u7b2c2\u56de\u3067\u3082\u3054\u7d39\u4ecb\u3057\u305f\u3088\u3046\u306b\u3001\u30ac\u30a4\u30c9RNA\u3092\u30c7\u30b6\u30a4\u30f3\u3057\u305f\u3068\u304d\u3001\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u3068\u306a\u308a\u3046\u308b\u914d\u5217\u304c\u3044\u304f\u3064\u304b\u30d4\u30c3\u30af\u30a2\u30c3\u30d7\u3055\u308c\u307e\u3057\u305f\u3002\u88681\u306f\u3001\u5f53\u793eInvitrogen\u2122 TrueDesign\u2122 Genomic Editor\u3092\u7528\u3044\u3066\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u7528\u306e\u30ac\u30a4\u30c9RNA\u3092\u30c7\u30b6\u30a4\u30f3\u3057\u305f\u3068\u304d\u306b\u8868\u793a\u3055\u308c\u305f\u3082\u306e\u3067\u3059\u30024\u7a2e\u985e\u306e\u30ac\u30a4\u30c9RNA\u304c\u30c7\u30b6\u30a4\u30f3\u3055\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u3053\u306e\u3046\u3061\u300cOff-Targets\u300d\u306e\u6b04\u306b\u306f\u6570\u5b57\u304c\u8a18\u8f09\u3055\u308c\u3066\u3044\u307e\u3059\u3002<b>\u3053\u306e\u6570\u5b57\u304c\u3001\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u3092\u5f15\u304d\u8d77\u3053\u3059\u53ef\u80fd\u6027\u306e\u3042\u308b\u914d\u5217\u306e\u6570\u3092\u8868\u3057\u3066\u3044\u307e\u3059\u3002<\/b><\/p>\n<p style=\"text-align: center\"><b>\u88681 TrueDesign Genomic Editor\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u914d\u5217\u306e\u8868\u793a(1)<\/b><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-14387\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/06\/cbf5b9f60da99bf791c0aa12a770f429.jpg\" alt=\"TrueDesign Genomic Editor\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u914d\u5217\u306e\u8868\u793a(1)\" width=\"561\" height=\"237\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/06\/cbf5b9f60da99bf791c0aa12a770f429.jpg 561w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/06\/cbf5b9f60da99bf791c0aa12a770f429-300x127.jpg 300w\" sizes=\"auto, (max-width: 561px) 100vw, 561px\" \/><\/p>\n<p>\u305f\u3068\u3048\u3070\u88681\u306e\u6700\u4e0a\u884c\u306e\u300c5\u300d\u3092\u30af\u30ea\u30c3\u30af\u3059\u308b\u3068\u3001\u88682\u306e\u3088\u3046\u306b\u914d\u5217\u60c5\u5831\u304c\u8868\u793a\u3055\u308c\u307e\u3059\u3002<\/p>\n<p style=\"text-align: center\"><b>\u88682 TrueDesign Genomic Editor\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u914d\u5217\u306e\u8868\u793a(2)<\/b><br \/>\n<img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-14388\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/06\/5e4896966c9944fcf00890f4502605bf.jpg\" alt=\"TrueDesign Genomic Editor\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u914d\u5217\u306e\u8868\u793a(2)\" width=\"446\" height=\"275\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/06\/5e4896966c9944fcf00890f4502605bf.jpg 446w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/06\/5e4896966c9944fcf00890f4502605bf-300x185.jpg 300w\" sizes=\"auto, (max-width: 446px) 100vw, 446px\" \/><\/p>\n<p><b>\u88682\u306b\u8868\u793a\u3055\u308c\u305f\u914d\u5217\u304c\u3001\u30b2\u30ce\u30e0\u4e0a\u3067\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u3092\u5f15\u304d\u8d77\u3053\u3059\u53ef\u80fd\u6027\u304c\u9ad8\u3044\u914d\u5217\u3067\u3059\u3002<\/b>\u305d\u306e\u305f\u3081\u3001\u3053\u3053\u306b\u8a18\u8f09\u3055\u308c\u305f\u914d\u5217\u3059\u3079\u3066\u3092\u30b7\u30fc\u30b1\u30f3\u30b9\u3067\u78ba\u8a8d\u3057\u3066\u304a\u304d\u307e\u3057\u3087\u3046\u3002<\/p>\n<p>\u307e\u305f\u306f\u3001\u540c\u3058\u304fTrueDesign Genomic Editor\u30c4\u30fc\u30eb\u306e\u30ac\u30a4\u30c9RNA\u6ce8\u6587\u76f4\u524d\u306e\u753b\u9762\u3067\u300c<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/gene-editing_bid_ts_2\/?CID=bid_syn_gns_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_lgn_kt_s00_gene_editing_bid_ts_6_Social_LAB#jump\" target=\"_blank\" rel=\"noopener\">Download Designs and Protocol<\/a>\u300d\u3092\u30af\u30ea\u30c3\u30af\u3057\u3001\u6ce8\u6587\u5185\u5bb9\u3092\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3055\u308c\u3066\u3044\u305f\u3089\u3001\u305d\u306e\u30d5\u30a1\u30a4\u30eb\u304b\u3089\u3082\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u914d\u5217\u304c\u78ba\u8a8d\u3067\u304d\u307e\u3059\u3002<\/p>\n<p>\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u53ef\u80fd\u6027\u306e\u3042\u308b\u914d\u5217\u304c\u78ba\u8a8d\u3067\u304d\u305f\u3089\u3001\u3053\u308c\u3089\u306e\u914d\u5217\u306b\u5bfe\u3057\u3066\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u30c7\u30b6\u30a4\u30f3\u3057\u3001\u30b7\u30fc\u30b1\u30f3\u30b5\u3067\u8aad\u307f\u53d6\u308a\u3001\u5909\u7570\u304c\u5165\u3063\u3066\u3044\u306a\u3044\u304b\u78ba\u8a8d\u3057\u307e\u3057\u3087\u3046\u3002\u5909\u7570\u304c\u5165\u3063\u3066\u3044\u308b\u682a\u306f\u3001\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u682a\u3068\u3057\u3066\u9664\u5916\u3059\u308b\u3053\u3068\u3092\u304a\u3059\u3059\u3081\u3057\u307e\u3059\u3002<\/p>\n<h2>\u305d\u3082\u305d\u3082\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u52b9\u679c\u3092\u9632\u3050\u306b\u306f\uff1f<\/h2>\n<p><span style=\"color: #3366ff\">A\u3055\u3093<\/span>\uff1a<br \/>\n\u5148\u8f29\u3001\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u914d\u5217\u4ed8\u8fd1\u3092\u30b7\u30fc\u30b1\u30f3\u30b9\u3067\u898b\u308b\u306e\u304c\u6016\u304f\u306a\u3063\u3066\u304d\u307e\u3057\u305f\u3002\u3082\u3057\u5909\u7570\u304c\u5165\u3063\u305f\u682a\u3059\u3079\u3066\u306b\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u304c\u5165\u3063\u3066\u3044\u305f\u3089\u3001\u5b9f\u9a13\u3084\u308a\u76f4\u3057\u3067\u3059\u304b\uff1f<\/p>\n<p><span style=\"color: #ff00ff\">\u5148\u8f29<\/span>\uff1a<br \/>\n\u5909\u7570\u3092\u5165\u308c\u308b\u3068\u3053\u308d\u304b\u3089\u3082\u30461\u56de\u304b\u3001\u30ac\u30a4\u30c9RNA\u3092\u4f5c\u308a\u76f4\u3057\u305f\u65b9\u304c\u826f\u3044\u30b1\u30fc\u30b9\u3082\u3042\u308b\u3088\u3002\u3067\u3082\u3084\u308a\u76f4\u3057\u306f\u9762\u5012\u3060\u3057\u3001\u3060\u304b\u3089\u3053\u305d\u3001\u3042\u3089\u304b\u3058\u3081\u5b9f\u9a13\u30c7\u30b6\u30a4\u30f3\u306e\u3068\u304d\u306b\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u52b9\u679c\u3092\u9632\u3050\u5bfe\u7b56\u3092\u7acb\u3066\u3066\u304a\u304f\u306e\u304c\u91cd\u8981\u306a\u3093\u3060\u3002<\/p>\n<p>\u4eca\u307e\u3067\u306e\u30b2\u30ce\u30e0\u7de8\u96c6\u30b7\u30ea\u30fc\u30ba\u306e\u7b2c1\u56de\uff5e\u7b2c5\u56de\u3067\u3001\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u52b9\u679c\u3092\u9632\u3050\u30dd\u30a4\u30f3\u30c8\u304c\u3044\u304f\u3064\u304b\u51fa\u3066\u304d\u307e\u3057\u305f\u3002\u5fa9\u7fd2\u3092\u517c\u306d\u3066\u30dd\u30a4\u30f3\u30c8\u3092\u307e\u3068\u3081\u308b\u3068\u3001\u4e0b\u8a18\u306e\u3088\u3046\u306b\u306a\u308a\u307e\u3059\u3002<\/p>\n<p><b>\uff1c\u30b2\u30ce\u30e0\u7de8\u96c6\uff08CRISPR\uff09\u306b\u304a\u3051\u308b\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u52b9\u679c\u3092\u9632\u3050\u65b9\u6cd5\uff1e<\/b><\/p>\n<ol>\n<li>\u30ac\u30a4\u30c9RNA\u306e3\u2019\u306e10\uff5e12\u5869\u57fa\uff08Seed\u9818\u57df\uff09\u306e\u7279\u7570\u6027\u3092\u9ad8\u3081\u308b<br \/>\n\uff08\u3064\u307e\u308a\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u4ee5\u5916\u306e\u914d\u5217\u304c\u3001Seed\u9818\u57df\u306b\u30df\u30b9\u30de\u30c3\u30c1\u3092\u6301\u3064\u3088\u3046\u306a\u914d\u5217\u3092\u63a2\u3059\uff09<\/li>\n<li>CAS9\u306fProtein\u65b9\u6cd5\u3092\u7528\u3044\u308b<\/li>\n<li>Cas9\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u3057\u3066\u3001Invitrogen\u2122 <a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/A50574?CID=bid_syn_gns_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_lgn_kt_s00_gene_editing_bid_ts_6_Social_LAB\" target=\"_blank\" rel=\"noopener\">TrueCut\u2122 HiFi Cas9 Protein<\/a>\u3092\u4f7f\u3046<\/li>\n<\/ol>\n<p><span style=\"color: #3366ff\">A\u3055\u3093<\/span>\uff1a<br \/>\n\u5148\u8f29\u30011\u30682\u306f\u7fd2\u3063\u305f\u5185\u5bb9\u3067\u3059\u304c\u30013\u306f\u521d\u8033\u3067\u3059\u3002TrueCut HiFi Cas9 Protein\u3063\u3066\u306a\u3093\u3067\u3057\u3087\u3046\u304b\u3002<\/p>\n<p><span style=\"color: #ff00ff\">\u5148\u8f29<\/span>\uff1a<br \/>\nTrueCut HiFi Cas9\u306f\u3001\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u52b9\u679c\u3092\u4f4e\u6e1b\u3055\u305b\u308b\u5de5\u592b\u304c\u3055\u308c\u3066\u3044\u308bCas9\u3060\u3088\u3002<\/p>\n<p>TrueCut HiFi Cas9 Protein\u306f\u3001\u5f93\u6765\u578b\u306eTrueCut Cas9\u306b\u5909\u7570\u3092\u7d44\u307f\u8fbc\u3080\u3053\u3068\u3067\u3001<a href=\"https:\/\/assets.thermofisher.com\/TFS-Assets\/BID\/Reference-Materials\/how-monitor-minimize-target-events-genome-editing-white-paper.pdf?CID=bid_syn_gns_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_lgn_kt_s00_gene_editing_bid_ts_6_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u52b9\u679c\u3092\u4f4e\u6e1b\u3055\u305b\u308b\u3088\u3046\u306b\u958b\u767a\u3055\u308c\u305fCas9<\/a>\u3067\u3059\u3002\u56f31\u306f\u5f93\u6765\u578b\u306eCas9\uff08wt-Cas9\uff09\u3068\u3001\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u4f4e\u6e1b\u578b\u306eTrueCut HiFi Cas9\u306e\u30aa\u30f3\u30bf\u30fc\u30b2\u30c3\u30c8\u52b9\u679c\u30fb\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u52b9\u679c\u306e\u6bd4\u7387\u3092\u307e\u3068\u3081\u305f\u3082\u306e\u3067\u3059\u3002\u7e26\u8ef8\u306f\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u30a4\u30d9\u30f3\u30c8\u7dcf\u6570\u3067\u3001\u5f93\u6765\u578bCas9\u3068\u6bd4\u8f03\u3057\u3066TrueCut HiFi Cas9\u3067\u306f\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u7dcf\u6570\u304c\u5287\u7684\u306b\u4f4e\u3044\u306e\u304c\u308f\u304b\u308a\u307e\u3059\u3002<\/p>\n<div id=\"attachment_14391\" style=\"width: 861px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-14391\" class=\"size-full wp-image-14391\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/06\/1efad1b5aa917a846fa5b3fc11367269.jpg\" alt=\"\u5f93\u6765\u578bCas9\u3068TrueCut HiFi Cas9\u306e\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u7dcf\u6570\u306e\u6bd4\u8f03\" width=\"851\" height=\"350\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/06\/1efad1b5aa917a846fa5b3fc11367269.jpg 851w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/06\/1efad1b5aa917a846fa5b3fc11367269-300x123.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/06\/1efad1b5aa917a846fa5b3fc11367269-768x316.jpg 768w\" sizes=\"auto, (max-width: 851px) 100vw, 851px\" \/><p id=\"caption-attachment-14391\" class=\"wp-caption-text\">\u56f31\u3000\u5f93\u6765\u578bCas9\u3068TrueCut HiFi Cas9\u306e\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u7dcf\u6570\u306e\u6bd4\u8f03<\/p><\/div>\n<p>\u306a\u304a\u3001TrueCut HiFi Cas9\u306f\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u4f4e\u6e1b\u306b\u306f\u5287\u7684\u306a\u52b9\u679c\u3092\u53ca\u307c\u3057\u307e\u3059\u304c\u3001\u5834\u5408\u306b\u3088\u3063\u3066\u3001\u809d\u5fc3\u306e\u30bf\u30fc\u30b2\u30c3\u30c8\u90e8\u5206\u3078\u306e\u5909\u7570\u5c0e\u5165\u52b9\u7387\u304c\u5f93\u6765\u578b\u306b\u6bd4\u3079\u3066\u308f\u305a\u304b\u306b\u4e0b\u304c\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<p>\u305d\u306e\u305f\u3081\u3001\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u52b9\u679c\u304c\u5341\u5206\u4f4e\u3044\u3068\u304d\u306b\u3001TrueCut HiFi Cas9\u3092\u7528\u3044\u308b\u5fc5\u8981\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u3057\u304b\u3057\u3001\u305f\u3068\u3048\u3070\u30ac\u30a4\u30c9RNA\u306e\u30c7\u30b6\u30a4\u30f3\u4e0a\u3001\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u52b9\u679c\u3092\u5f15\u304d\u8d77\u3053\u3059\u914d\u5217\u6570\u304c\u591a\u3044\u5834\u5408\u3084\u3001\u5b9f\u9a13\u306e\u90fd\u5408\u4e0a\u3001\u4f9b\u4e0e\u65b9\u6cd5\u3068\u3057\u3066Protein\u65b9\u6cd5\u304c\u63a1\u7528\u3067\u304d\u306a\u3044\u3068\u304d\u3001\u901a\u5e38\u306eTrueCut Cas9\u3067\u5b9f\u65bd\u3057\u305f\u7d50\u679c\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u52b9\u679c\u304c\u9ad8\u304b\u3063\u305f\u3068\u304d\u306a\u3069\u306b\u691c\u8a0e\u3057\u307e\u3057\u3087\u3046\u3002\u305d\u3046\u3044\u3063\u305f\u3068\u304d\u306f\u3001TrueCut HiFi Cas9\u304c\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u5927\u5e45\u306a\u8efd\u6e1b\u306b\u6d3b\u8e8d\u3092\u3057\u3066\u304f\u308c\u307e\u3059\u3002<\/p>\n<h2>\u307e\u3068\u3081\uff1a\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u914d\u5217\u306f\u5fc5\u305a\u78ba\u8a8d\u3057\u3088\u3046<\/h2>\n<p>\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u78ba\u8a8d\u306b\u306f\u3001\u30ac\u30a4\u30c9RNA\u30c7\u30b6\u30a4\u30f3\u30c4\u30fc\u30eb\u3067\u30d4\u30c3\u30af\u30a2\u30c3\u30d7\u3055\u308c\u305f\u914d\u5217\u4ed8\u8fd1\u3092\u78ba\u8a8d\u3057\u307e\u3057\u3087\u3046\u3002\u307e\u305f\u3001\u5b9f\u9a13\u306e\u3084\u308a\u76f4\u3057\u304c\u6975\u529b\u751f\u3058\u306a\u3044\u3088\u3046\u306b\u3001\u5b9f\u9a13\u8a08\u753b\u3092\u7acb\u3066\u308b\u3068\u304d\u306b\u306f\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u3092\u9632\u3050\u65b9\u6cd5\u3092\u306a\u308b\u3079\u304f\u63a1\u7528\u3059\u308b\u3053\u3068\u3092\u304a\u3059\u3059\u3081\u3057\u307e\u3059\u3002<\/p>\n<p><span style=\"color: #3366ff\">A\u3055\u3093<\/span>\uff1a<br \/>\n\u50d5\u306e\u5b9f\u9a13\u306f\u7121\u4e8b\u306b\u7d42\u3048\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3057\u305f\u3002<br \/>\n\u305d\u3046\u3060\u3001\u30ce\u30c3\u30af\u30a4\u30f3\u306b\u3064\u3044\u3066\u3082\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\u306a\u3002<\/p>\n<p>\u3055\u3066\u3001\u7b2c1\u56de\uff5e\u7b2c6\u56de\u3092\u901a\u3057\u3066\u3001A\u3055\u3093\u306e\u521d\u3081\u3066\u306e\u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\u306f\u9806\u8abf\u306b\u7d42\u3048\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3057\u305f\u3002\u3057\u304b\u3057\u3001\u4eca\u307e\u3067\u306e\u9023\u8f09\u306f\u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\u306e\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u5b9f\u9a13\u3092\u524d\u63d0\u3068\u3057\u3066\u3044\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u3001<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/gene-editing_bid_ts_7\/?CID=bid_syn_gns_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_lgn_kt_s00_gene_editing_bid_ts_6_Social_LAB\" rel=\"noopener\" target=\"_blank\">\u6b21\u56de\u6700\u7d42\u56de<\/a>\u3067\u306f\u3001\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u306b\u6bd4\u3079\u3066\u307e\u3060\u307e\u3060\u6b74\u53f2\u304c\u6d45\u3044\u3051\u308c\u3069\u78ba\u5b9f\u306b\u8ad6\u6587\u6570\u304c\u5897\u3048\u3066\u3044\u308b\u30ce\u30c3\u30af\u30a4\u30f3\u5b9f\u9a13\u306b\u3064\u3044\u3066\u3001\u30dd\u30a4\u30f3\u30c8\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n<p>\uff1c\u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\u30b7\u30ea\u30fc\u30ba\uff1e<br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/gene-editing_bid_ts_1\/?CID=bid_syn_gns_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_lgn_kt_s00_gene_editing_bid_ts_6_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u305d\u3046\u3044\u3046\u3053\u3068\u3060\u3063\u305f\u306e\u304b ! \u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\uff08CRISPR\/Cas9\uff09 \uff5e\u7b2c1\u56de\u3000CRISPR\/Cas9\u30b7\u30b9\u30c6\u30e0\u306e\u539f\u7406\uff5e<\/a><br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/gene-editing_bid_ts_2\/?CID=bid_syn_gns_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_lgn_kt_s00_gene_editing_bid_ts_6_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u305d\u3046\u3044\u3046\u3053\u3068\u3060\u3063\u305f\u306e\u304b ! \u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\uff08CRISPR\/Cas9\uff09 \uff5e\u7b2c2\u56de\u3000\u30ac\u30a4\u30c9RNA\u306e\u30c7\u30b6\u30a4\u30f3\uff5e<\/a><br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/gene-editing_bid_ts_3\/?CID=bid_syn_gns_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_lgn_kt_s00_gene_editing_bid_ts_6_Social_LAB\" target=\"_blank\" 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