{"id":14608,"date":"2023-07-04T11:21:27","date_gmt":"2023-07-04T02:21:27","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/?p=14608"},"modified":"2025-04-15T16:06:02","modified_gmt":"2025-04-15T07:06:02","slug":"qpcr_workshop_gsd_ts_3","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr_workshop_gsd_ts_3\/","title":{"rendered":"\u3010\u3084\u3063\u3066\u307f\u305f\u3011\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3000\u30e9\u30f3\u30e2\u30fc\u30c9\u3067\u7d50\u679c\u306b\u5dee\u304c\u3067\u308b\u304b\u6bd4\u8f03\u3057\u3066\u307f\u305f"},"content":{"rendered":"<h2>\u306f\u3058\u3081\u306b<\/h2>\n<p>\u5f53\u793e\u306e\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u88c5\u7f6e\u306b\u306fFast\u30e2\u30fc\u30c9\u3068Standard\u30e2\u30fc\u30c9\u306e2\u7a2e\u985e\u306e\u30e9\u30f3\u30e2\u30fc\u30c9\u304c\u3042\u308a\u307e\u3059\u3002\u71b1\u5909\u6027\u306e\u30b9\u30c6\u30c3\u30d7\u3084\u30a2\u30cb\u30fc\u30ea\u30f3\u30b0\u30fb\u4f38\u9577\u53cd\u5fdc\u30b9\u30c6\u30c3\u30d7\u306e\u53cd\u5fdc\u6642\u9593\u3068\u6e29\u5ea6\u306e\u4e0a\u3052\u4e0b\u3052\u306e\u30b9\u30d4\u30fc\u30c9\u304c\u7570\u306a\u308a\u307e\u3059\u3002\u305d\u306e\u305f\u3081Fast\u30e2\u30fc\u30c9\u3067\u306f\u7d0430\u5206\u3001Standard\u30e2\u30fc\u30c9\u3067\u306f\u7d041\u6642\u9593\u534a\u3068\u30e9\u30f3\u6642\u9593\u304c\u5927\u304d\u304f\u5909\u308f\u308a\u307e\u3059\u3002Fast\u30e2\u30fc\u30c9\u306f\u30e9\u30f3\u6642\u9593\u304c\u77ed\u3044\u53cd\u9762\u3001\u77ed\u3044\u53cd\u5fdc\u6642\u9593\u3067\u554f\u984c\u304c\u306a\u3044\u306e\u304b\u4e0d\u5b89\u306b\u601d\u3046\u65b9\u3082\u3044\u3089\u3063\u3057\u3083\u308b\u304b\u3082\u3057\u308c\u307e\u305b\u3093\u3002\u5b9f\u969b\u306b\u300c\u4f38\u9577\u53cd\u5fdc20\u79d2\u3067\u672c\u5f53\u306b\u5927\u4e08\u592b\uff1f\u300d\u3068\u3054\u8cea\u554f\u3092\u3046\u3051\u308b\u3053\u3068\u3082\u3042\u308a\u307e\u3059\u3002\u4eca\u56de\u306e\u8a18\u4e8b\u3067\u306fFast\u30e2\u30fc\u30c9\u306e\u8a66\u85ac\u3092\u3042\u3048\u3066Standard\u30e2\u30fc\u30c9\u3067\u30e9\u30f3\u3057\u3001\u7d50\u679c\u306b\u5dee\u304c\u3042\u308b\u306e\u304b\u78ba\u8a8d\u3057\u3066\u307f\u307e\u3057\u305f\u3002<\/p>\n<p>\u3053\u3093\u306a\u65b9\u306b\u304a\u52e7\u3081\u3067\u3059\u3002<\/p>\n<ul>\n<li>\u30e9\u30f3\u30e2\u30fc\u30c9\u306e\u9078\u629e\u306b\u8ff7\u3063\u3066\u3044\u308b\u65b9<\/li>\n<li>Fast\u30e2\u30fc\u30c9\u3067\u5341\u5206\u306bPCR\u53cd\u5fdc\u304c\u9032\u3093\u3067\u3044\u308b\u306e\u304b\u4e0d\u5b89\u306a\u65b9<\/li>\n<li>Fast\u30e2\u30fc\u30c9\u306e\u8a66\u85ac\u3092\u691c\u8a0e\u3055\u308c\u3066\u3044\u308b\u65b9<\/li>\n<\/ul>\n<h2>\u6750\u6599\u3068\u65b9\u6cd5<\/h2>\n<p>Fast\u30e2\u30fc\u30c9\u7528\u8a66\u85ac\u3068\u3057\u3066Applied Biosystems\u2122 <a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/4444556?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_og_s00_qpcr_workshop_gsd_ts_3_Social_LAB\" target=\"_blank\" rel=\"noopener\">TaqMan\u2122 Fast Advanced Master Mix<\/a>\u3092\u4f7f\u7528\u3057\u307e\u3057\u305f\u3002\u88fd\u54c1\u306e\u30d7\u30ed\u30c8\u30b3\u30eb\u306b\u5f93\u304420 \u00b5L\u5bb9\u91cf\u306e\u53cd\u5fdc\u6db2\u3092\u8abf\u88fd\u3057\u307e\u3057\u305f\u300250 ng\/well\u306b\u8abf\u88fd\u3055\u308c\u305fcDNA\u30924\u500d\u3082\u3057\u304f\u306f3\u500d\u5e0c\u91c8\u3057\u8a086\u70b9\u306e\u5e0c\u91c8\u7cfb\u5217\u3092\u4f5c\u6210\u3001\u92f3\u578b\u3068\u3057\u3066\u4f7f\u7528\u3057\u307e\u3057\u305f\u3002\u30ec\u30d7\u30ea\u30b1\u30fc\u30c8\u306f3\u3068\u3057\u307e\u3057\u305f\u3002Applied Biosystems\u2122 <a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/pcr\/real-time-pcr\/real-time-pcr-assays.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_og_s00_qpcr_workshop_gsd_ts_3_Social_LAB\" target=\"_blank\" rel=\"noopener\">TaqMan\u2122 Gene Expression Assay<\/a> (20x) \u3092\u3082\u3061\u3044\u3066GAPDH\u3001ACTB\u3001IL-8\u3001COL1A1\u3001ACTA2\u3001ALDHA1\u306e6\u3064\u306e\u907a\u4f1d\u5b50\u3092\u6e2c\u5b9a\u3057\u307e\u3057\u305f\u3002\u53cd\u5fdc\u6761\u4ef6\u306f\u4e0b\u8a18\u306e\u901a\u308a\u3067\u3059\uff08\u56f3\uff11\u3001\u56f32\uff09\u3002\u88c5\u7f6e\u306f<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/pcr\/real-time-pcr\/real-time-pcr-instruments\/quantstudio-systems\/models\/quantstudio-6-7-pro.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_og_s00_qpcr_workshop_gsd_ts_3_Social_LAB\" target=\"_blank\" rel=\"noopener\">Applied Biosystems\u2122 QuantStudio\u2122 7 Pro\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u30b7\u30b9\u30c6\u30e0<\/a>\u3092\u4f7f\u7528\u3057\u307e\u3057\u305f\u3002<\/p>\n<p>Standard\u30e2\u30fc\u30c9\u306e\u30d7\u30ed\u30b0\u30e9\u30e0 \u30e9\u30f3\u6240\u8981\u6642\u9593\u20261\u6642\u959321\u5206<\/p>\n<div id=\"attachment_14610\" style=\"width: 494px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-14610\" class=\"size-full wp-image-14610\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/07\/qpcr_workshop_gsd_ts_3-01.png\" alt=\"Standard\u30e2\u30fc\u30c9\u306e\u30d7\u30ed\u30b0\u30e9\u30e0\" width=\"484\" height=\"371\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/07\/qpcr_workshop_gsd_ts_3-01.png 484w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/07\/qpcr_workshop_gsd_ts_3-01-300x230.png 300w\" sizes=\"auto, (max-width: 484px) 100vw, 484px\" \/><p id=\"caption-attachment-14610\" class=\"wp-caption-text\">\u56f31\uff0eStandard\u30e2\u30fc\u30c9\u306e\u30d7\u30ed\u30b0\u30e9\u30e0<\/p><\/div>\n<p>Fast\u30e2\u30fc\u30c9\u306e\u30d7\u30ed\u30b0\u30e9\u30e0 \u30e9\u30f3\u6240\u8981\u6642\u9593\u202632\u5206<\/p>\n<div id=\"attachment_14611\" style=\"width: 484px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-14611\" class=\"size-full wp-image-14611\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/07\/qpcr_workshop_gsd_ts_3-02.png\" alt=\"Fast\u30e2\u30fc\u30c9\u306e\u30d7\u30ed\u30b0\u30e9\u30e0\" width=\"474\" height=\"366\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/07\/qpcr_workshop_gsd_ts_3-02.png 474w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/07\/qpcr_workshop_gsd_ts_3-02-300x232.png 300w\" sizes=\"auto, (max-width: 474px) 100vw, 474px\" \/><p id=\"caption-attachment-14611\" class=\"wp-caption-text\">\u56f32\uff0eFast\u30e2\u30fc\u30c9\u306e\u30d7\u30ed\u30b0\u30e9\u30e0<\/p><\/div>\n<h2>\u7d50\u679c\u3068\u8003\u5bdf<\/h2>\n<p>\u305d\u308c\u3067\u306f\u65e9\u901f\u7d50\u679c\u3092\u898b\u3066\u307f\u307e\u3057\u3087\u3046\u3002<br \/>\n\u5404\u30bf\u30fc\u30b2\u30c3\u30c8\u907a\u4f1d\u5b50\u3054\u3068\u306bcDNA\u91cf\u3092\u6a2a\u8ef8\u3001Ct\u5024\u3092\u7e26\u8ef8\u306b\u30d7\u30ed\u30c3\u30c8\u3057\u307e\u3057\u305f\u3002Standard\u30e2\u30fc\u30c9\u306e\u9752\u3044\u30d7\u30ed\u30c3\u30c8\u3068Fast\u30e2\u30fc\u30c9\u306e\u30aa\u30ec\u30f3\u30b8\u306e\u30d7\u30ed\u30c3\u30c8\u306f\u975e\u5e38\u306b\u3088\u304f\u4e00\u81f4\u3057\u307e\u3057\u305f\uff08\u56f33\uff09\u3002\u30c6\u30af\u30cb\u30ab\u30eb\u30ec\u30d7\u30ea\u30b1\u30fc\u30c8\u306e3\u30a6\u30a7\u30eb\u306e\u5e73\u5747\u5024\u3092\u6bd4\u8f03\u3057\u3066\u3082\u5dee\u306f\u308f\u305a\u304b\u3067\u3057\u305f\uff08\u88681\uff09\u3002<\/p>\n<div id=\"attachment_14609\" style=\"width: 1004px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-14609\" class=\"size-full wp-image-14609\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/07\/qpcr_workshop_gsd_ts_3-03.png\" alt=\"Ct\u5024\u306e\u30d7\u30ed\u30c3\u30c8\u56f3\" width=\"994\" height=\"905\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/07\/qpcr_workshop_gsd_ts_3-03.png 994w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/07\/qpcr_workshop_gsd_ts_3-03-300x273.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/07\/qpcr_workshop_gsd_ts_3-03-768x699.png 768w\" sizes=\"auto, (max-width: 994px) 100vw, 994px\" \/><p id=\"caption-attachment-14609\" class=\"wp-caption-text\">\u56f33\uff0eCt\u5024\u306e\u30d7\u30ed\u30c3\u30c8\u56f3<\/p><\/div>\n<table width=\"342\">\n<caption><b>\u88681\uff0e\u5404\u30b5\u30f3\u30d7\u30eb\u306b\u304a\u3051\u308bCt\u5024\u306e\u5e73\u5747\u5024<\/b><\/caption>\n<tbody>\n<tr bgcolor=\"aliceblue\">\n<td width=\"78\">ACTB<\/td>\n<td width=\"44\">S1<\/td>\n<td width=\"44\">S2<\/td>\n<td width=\"44\">S3<\/td>\n<td width=\"44\">S4<\/td>\n<td width=\"44\">S5<\/td>\n<td width=\"44\">S6<\/td>\n<\/tr>\n<tr>\n<td width=\"78\">Fast<\/td>\n<td width=\"44\">17.3<\/td>\n<td width=\"44\">19.1<\/td>\n<td width=\"44\">21.2<\/td>\n<td width=\"44\">23.3<\/td>\n<td width=\"44\">25.4<\/td>\n<td width=\"44\">27.5<\/td>\n<\/tr>\n<tr>\n<td width=\"78\">Standard<\/td>\n<td width=\"44\">17.0<\/td>\n<td width=\"44\">19.2<\/td>\n<td width=\"44\">21.3<\/td>\n<td width=\"44\">23.3<\/td>\n<td width=\"44\">25.4<\/td>\n<td width=\"44\">27.5<\/td>\n<\/tr>\n<tr>\n<td width=\"78\"><\/td>\n<td width=\"44\"><\/td>\n<td width=\"44\"><\/td>\n<td width=\"44\"><\/td>\n<td width=\"44\"><\/td>\n<td width=\"44\"><\/td>\n<td width=\"44\"><\/td>\n<\/tr>\n<tr bgcolor=\"aliceblue\">\n<td width=\"78\">GAPDH<\/td>\n<td width=\"44\">S1<\/td>\n<td width=\"44\">S2<\/td>\n<td width=\"44\">S3<\/td>\n<td width=\"44\">S4<\/td>\n<td width=\"44\">S5<\/td>\n<td width=\"44\">S6<\/td>\n<\/tr>\n<tr>\n<td width=\"78\">Fast<\/td>\n<td width=\"44\">16.9<\/td>\n<td width=\"44\">19.0<\/td>\n<td width=\"44\">21.0<\/td>\n<td width=\"44\">23.2<\/td>\n<td width=\"44\">25.3<\/td>\n<td width=\"44\">27.5<\/td>\n<\/tr>\n<tr>\n<td width=\"78\">Standard<\/td>\n<td width=\"44\">16.8<\/td>\n<td width=\"44\">18.9<\/td>\n<td width=\"44\">20.9<\/td>\n<td width=\"44\">23.1<\/td>\n<td width=\"44\">25.2<\/td>\n<td width=\"44\">27.4<\/td>\n<\/tr>\n<tr>\n<td width=\"78\"><\/td>\n<td width=\"44\"><\/td>\n<td width=\"44\"><\/td>\n<td width=\"44\"><\/td>\n<td width=\"44\"><\/td>\n<td width=\"44\"><\/td>\n<td width=\"44\"><\/td>\n<\/tr>\n<tr bgcolor=\"aliceblue\">\n<td width=\"78\">IL-8<\/td>\n<td width=\"44\">S1<\/td>\n<td width=\"44\">S2<\/td>\n<td width=\"44\">S3<\/td>\n<td width=\"44\">S4<\/td>\n<td width=\"44\">S5<\/td>\n<td width=\"44\">S6<\/td>\n<\/tr>\n<tr>\n<td width=\"78\">Fast<\/td>\n<td width=\"44\">24.5<\/td>\n<td width=\"44\">26.7<\/td>\n<td width=\"44\">28.8<\/td>\n<td width=\"44\">30.8<\/td>\n<td width=\"44\">33.0<\/td>\n<td width=\"44\">34.9<\/td>\n<\/tr>\n<tr>\n<td width=\"78\">Standard<\/td>\n<td width=\"44\">24.2<\/td>\n<td width=\"44\">26.4<\/td>\n<td width=\"44\">28.3<\/td>\n<td width=\"44\">30.4<\/td>\n<td width=\"44\">32.3<\/td>\n<td width=\"44\">34.8<\/td>\n<\/tr>\n<tr>\n<td width=\"78\"><\/td>\n<td width=\"44\"><\/td>\n<td width=\"44\"><\/td>\n<td width=\"44\"><\/td>\n<td width=\"44\"><\/td>\n<td width=\"44\"><\/td>\n<td width=\"44\"><\/td>\n<\/tr>\n<tr bgcolor=\"aliceblue\">\n<td width=\"78\">COL1A1<\/td>\n<td width=\"44\">S1<\/td>\n<td width=\"44\">S2<\/td>\n<td width=\"44\">S3<\/td>\n<td width=\"44\">S4<\/td>\n<td width=\"44\">S5<\/td>\n<td width=\"44\">S6<\/td>\n<\/tr>\n<tr>\n<td width=\"78\">Fast<\/td>\n<td width=\"44\">22.8<\/td>\n<td width=\"44\">24.4<\/td>\n<td width=\"44\">26.0<\/td>\n<td width=\"44\">27.6<\/td>\n<td width=\"44\">29.4<\/td>\n<td width=\"44\">30.8<\/td>\n<\/tr>\n<tr>\n<td width=\"78\">Standard<\/td>\n<td width=\"44\">23.2<\/td>\n<td width=\"44\">24.8<\/td>\n<td width=\"44\">26.4<\/td>\n<td width=\"44\">28.0<\/td>\n<td width=\"44\">29.6<\/td>\n<td width=\"44\">31.2<\/td>\n<\/tr>\n<tr>\n<td width=\"78\"><\/td>\n<td width=\"44\"><\/td>\n<td width=\"44\"><\/td>\n<td width=\"44\"><\/td>\n<td width=\"44\"><\/td>\n<td width=\"44\"><\/td>\n<td width=\"44\"><\/td>\n<\/tr>\n<tr bgcolor=\"aliceblue\">\n<td width=\"78\">ACTA2<\/td>\n<td width=\"44\">S1<\/td>\n<td width=\"44\">S2<\/td>\n<td width=\"44\">S3<\/td>\n<td width=\"44\">S4<\/td>\n<td width=\"44\">S5<\/td>\n<td width=\"44\">S6<\/td>\n<\/tr>\n<tr>\n<td width=\"78\">Fast<\/td>\n<td width=\"44\">25.2<\/td>\n<td width=\"44\">26.8<\/td>\n<td width=\"44\">28.6<\/td>\n<td width=\"44\">30.3<\/td>\n<td width=\"44\">32.0<\/td>\n<td width=\"44\">33.5<\/td>\n<\/tr>\n<tr>\n<td width=\"78\">Standard<\/td>\n<td width=\"44\">25.3<\/td>\n<td width=\"44\">26.9<\/td>\n<td width=\"44\">28.6<\/td>\n<td width=\"44\">30.3<\/td>\n<td width=\"44\">31.9<\/td>\n<td width=\"44\">33.7<\/td>\n<\/tr>\n<tr>\n<td width=\"78\"><\/td>\n<td width=\"44\"><\/td>\n<td width=\"44\"><\/td>\n<td width=\"44\"><\/td>\n<td width=\"44\"><\/td>\n<td width=\"44\"><\/td>\n<td width=\"44\"><\/td>\n<\/tr>\n<tr bgcolor=\"aliceblue\">\n<td width=\"78\">ALDH1A1<\/td>\n<td width=\"44\">S1<\/td>\n<td width=\"44\">S2<\/td>\n<td width=\"44\">S3<\/td>\n<td width=\"44\">S4<\/td>\n<td width=\"44\">S5<\/td>\n<td width=\"44\">S6<\/td>\n<\/tr>\n<tr>\n<td width=\"78\">Fast<\/td>\n<td width=\"44\">23.7<\/td>\n<td width=\"44\">25.4<\/td>\n<td width=\"44\">27.1<\/td>\n<td width=\"44\">28.7<\/td>\n<td width=\"44\">30.3<\/td>\n<td width=\"44\">31.9<\/td>\n<\/tr>\n<tr>\n<td width=\"78\">Standard<\/td>\n<td width=\"44\">23.7<\/td>\n<td width=\"44\">25.4<\/td>\n<td width=\"44\">27.1<\/td>\n<td width=\"44\">28.6<\/td>\n<td width=\"44\">30.2<\/td>\n<td width=\"44\">32.1<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>\u691c\u91cf\u7dda\u306e\u7cbe\u5ea6\u3092\u3042\u3089\u308f\u3059R\u306e2\u4e57\u5024\u3084\u5897\u5e45\u52b9\u7387\u306b\u3064\u3044\u3066\u975e\u5e38\u306b\u8fd1\u3044\u5024\u3068\u306a\u308a\u307e\u3057\u305f\uff08\u88682\uff09\u3002\u53cd\u5fdc\u6642\u9593\u304c\u77ed\u7e2e\u3055\u308c\u305fFast\u30e2\u30fc\u30c9\u3067\u3082Standard\u30e2\u30fc\u30c9\u3068\u540c\u7b49\u306e\u7d50\u679c\u3092\u5f97\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3057\u305f\u3002Fast\u30e2\u30fc\u30c9\u3067\u3082PCR\u53cd\u5fdc\u304c\u5341\u5206\u306b\u9032\u3080\u3068\u5206\u304b\u308a\u307e\u3057\u305f\u3002<br \/>\n\u203b\u304a\u4f7f\u3044\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u30bb\u30c3\u30c8\u3001TaqMan Assay\u306b\u3088\u3063\u3066\u306f\u30e9\u30f3\u30e2\u30fc\u30c9\u306b\u3088\u308b\u5f71\u97ff\u304c\u3067\u308b\u53ef\u80fd\u6027\u3082\u3042\u308a\u307e\u3059\u3002<\/p>\n<table width=\"387\">\n<caption><b>\u88682\uff0e\u691c\u91cf\u7dda\u304b\u3089\u7b97\u51fa\u3055\u308c\u305fPCR\u52b9\u7387\u3068R2\u5024<\/b><\/caption>\n<tbody>\n<tr>\n<td width=\"72\"><\/td>\n<td style=\"text-align: center\" colspan=\"2\" width=\"157\">Fast\u30e2\u30fc\u30c9<\/td>\n<td style=\"text-align: center\" colspan=\"2\" width=\"157\">Standard\u30e2\u30fc\u30c9<\/td>\n<\/tr>\n<tr>\n<td width=\"72\"><\/td>\n<td width=\"106\">PCR\u52b9\u7387(%)<\/td>\n<td width=\"51\">R<sup>2<\/sup><\/td>\n<td width=\"106\">PCR\u52b9\u7387(%)<\/td>\n<td width=\"51\">R<sup>2<\/sup><\/td>\n<\/tr>\n<tr>\n<td width=\"72\">ACTB<\/td>\n<td width=\"106\">95.99<\/td>\n<td width=\"51\">1.000<\/td>\n<td width=\"106\">93.95<\/td>\n<td width=\"51\">1.000<\/td>\n<\/tr>\n<tr>\n<td width=\"72\">GAPDH<\/td>\n<td width=\"106\">92.28<\/td>\n<td width=\"51\">1.000<\/td>\n<td width=\"106\">92.12<\/td>\n<td width=\"51\">1.000<\/td>\n<\/tr>\n<tr>\n<td width=\"72\">IL-8<\/td>\n<td width=\"106\">94.44<\/td>\n<td width=\"51\">0.998<\/td>\n<td width=\"106\">95.34<\/td>\n<td width=\"51\">0.995<\/td>\n<\/tr>\n<tr>\n<td width=\"72\">COL1A1<\/td>\n<td width=\"106\">99.02<\/td>\n<td width=\"51\">1.000<\/td>\n<td width=\"106\">96.26<\/td>\n<td width=\"51\">0.997<\/td>\n<\/tr>\n<tr>\n<td width=\"72\">ACTA2<\/td>\n<td width=\"106\">92.41<\/td>\n<td width=\"51\">0.994<\/td>\n<td width=\"106\">91.88<\/td>\n<td width=\"51\">0.998<\/td>\n<\/tr>\n<tr>\n<td width=\"72\">ALDHA1<\/td>\n<td width=\"106\">94.25<\/td>\n<td width=\"51\">0.997<\/td>\n<td width=\"106\">92.12<\/td>\n<td width=\"51\">0.999<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h2>Fast\u30e2\u30fc\u30c9\u5bfe\u5fdc\u8a66\u85ac\u306e\u3054\u7d39\u4ecb<\/h2>\n<p>\u6700\u5f8c\u306b\u3001\u5f53\u793e\u3067\u304a\u3059\u3059\u3081\u3057\u3066\u3044\u308bFast\u30e2\u30fc\u30c9\u5bfe\u5fdc\u306eMaster Mix\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002TaqMan Assay\u5b9f\u9a13\u7cfb\u306e\u304a\u3059\u3059\u3081\u88fd\u54c1\u3067\u3001\u691c\u51fa\u611f\u5ea6\u3001\u7cbe\u5ea6\u3001\u30c0\u30a4\u30ca\u30df\u30c3\u30af\u30ec\u30f3\u30b8\u3001\u7279\u7570\u6027\u306b\u512a\u308c\u307e\u3059\u3002\u4eca\u56de\u306e\u5b9f\u9a13\u3067\u3082\u4f7f\u7528\u3057\u307e\u3057\u305f\u3002<br \/>\n<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/jp\/ja\/4444556?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_og_s00_qpcr_workshop_gsd_ts_3_Social_LAB\" target=\"_blank\" rel=\"noopener\">TaqMan Fast Advanced Master Mix<\/a><\/p>\n<p>SYBR\u2122 Green\u5b9f\u9a13\u7cfb\u306e\u304a\u3059\u3059\u3081\u88fd\u54c1\u3067\u3059\u3002Fast\u30e2\u30fc\u30c9\u306b\u5bfe\u5fdc\u3057\u3066\u304a\u308a\u3001\u7279\u7570\u6027\u3082\u9ad8\u3044\u8a66\u85ac\u3067\u3059\u3002<br \/>\n<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/A25741?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_og_s00_qpcr_workshop_gsd_ts_3_Social_LAB\" target=\"_blank\" rel=\"noopener\">Applied Biosystems\u2122 PowerUp\u2122 SYBR\u2122 Green Master Mix<\/a><\/p>\n<h2>\u307e\u3068\u3081<\/h2>\n<p>\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306eFast\u30e2\u30fc\u30c9\u3068Standard\u30e2\u30fc\u30c9\u306e\u3069\u3061\u3089\u3092\u4f7f\u3063\u3066\u3082\u7d50\u679c\u306b\u5dee\u304c\u51fa\u306a\u3044\u3053\u3068\u304c\u308f\u304b\u308a\u307e\u3057\u305f\u3002\u3082\u3057\u3001Fast\u30e2\u30fc\u30c9\u5bfe\u5fdc\u306e\u8a66\u85ac\u3092\u304a\u4f7f\u3044\u306e\u5834\u5408\u306f\u3001\u30e9\u30f3\u6642\u9593\u30921\u6642\u9593\u307b\u3069\u77ed\u304f\u3067\u304d\u307e\u3059\u306e\u3067\u3001\u305c\u3072Fast\u30e2\u30fc\u30c9\u3067\u6e2c\u5b9a\u3057\u3066\u307f\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n<p>\u5f53\u793e\u3067\u306fRNA\u62bd\u51fa\u3084\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3001\u4ed6\u306b\u3082\u7d30\u80de\u57f9\u990a\u3001\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306a\u3069\u3001\u5b9f\u969b\u306b\u5b9f\u9a13\uff08\u5b9f\u7fd2\uff09\u3092\u884c\u3044\u3064\u3064\u5b66\u3079\u308b\u5404\u7a2e<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/technical-resources\/trainings\/lifescience-training.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_og_s00_qpcr_workshop_gsd_ts_3_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30cf\u30f3\u30ba\u30aa\u30f3\u30c8\u30ec\u30fc\u30cb\u30f3\u30b0<\/a>\u3092\u958b\u50ac\u3057\u3066\u3044\u307e\u3059\u3002\u305d\u306e\u4e2d\u3067\u4eca\u56de\u306e\u3088\u3046\u306a\u5b9f\u9a13\u7d50\u679c\u3082\u3054\u7d39\u4ecb\u3057\u3066\u3044\u307e\u3059\u306e\u3067\u3001\u3053\u308c\u304b\u3089\u65b0\u3057\u3044\u5b9f\u9a13\u3092\u59cb\u3081\u3089\u308c\u308b\u65b9\u3001\u3088\u308a\u7406\u89e3\u3092\u6df1\u3081\u305f\u3044\u65b9\u306f\u305c\u3072\u3054\u53c2\u52a0\u304f\u3060\u3055\u3044\uff01<\/p>\n<p>\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u7406\u8ad6\u3084\u5b9f\u9a13\u30c7\u30b6\u30a4\u30f3\u306e\u8a2d\u8a08\u306a\u3069\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u57fa\u790e\u77e5\u8b58\u304c\u3053\u306e1\u518a\u3067\u308f\u304b\u308b\u300c\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u30cf\u30f3\u30c9\u30d6\u30c3\u30af\u300d\u3092\u914d\u5e03\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/real-time-pcr-handbook-form.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_og_s00_qpcr_workshop_gsd_ts_3_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/p>\n<p>\u307e\u305f\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u5b9f\u9a13\u3067\u306e\u300c\u56f0\u3063\u305f\u300d\u3092\u89e3\u6c7a\u3059\u308b\u30c8\u30e9\u30d6\u30eb\u30b7\u30e5\u30fc\u30c6\u30a3\u30f3\u30b0\u30ac\u30a4\u30c9\u3082\u914d\u5e03\u3057\u3066\u3044\u307e\u3059\u3002\u4f75\u305b\u3066\u3054\u3089\u3093\u304f\u3060\u3055\u3044\u3002<\/p>\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/pcr-system-troubleshooting.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_og_s00_qpcr_workshop_gsd_ts_3_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/p>\n<p>&nbsp;<\/p>\n<p>\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u7528\u306b\u306f\u4f7f\u7528\u3044\u305f\u3060\u3051\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u306f\u3058\u3081\u306b \u5f53\u793e\u306e\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u88c5\u7f6e\u306b\u306fFast\u30e2\u30fc\u30c9\u3068Standard\u30e2\u30fc\u30c9\u306e2\u7a2e\u985e\u306e\u30e9\u30f3\u30e2\u30fc\u30c9\u304c\u3042\u308a\u307e\u3059\u3002\u71b1\u5909\u6027\u306e\u30b9\u30c6\u30c3\u30d7\u3084\u30a2\u30cb\u30fc\u30ea\u30f3\u30b0\u30fb\u4f38\u9577\u53cd\u5fdc\u30b9\u30c6\u30c3\u30d7\u306e\u53cd\u5fdc\u6642\u9593\u3068\u6e29\u5ea6\u306e\u4e0a\u3052\u4e0b\u3052\u306e\u30b9\u30d4\u30fc\u30c9\u304c\u7570\u306a\u308a\u307e\u3059\u3002\u305d\u306e\u305f\u3081F<\/p>\n","protected":false},"author":975,"featured_media":12035,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[156],"tags":[28,7],"division":[],"class_list":{"0":"post-14608","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-mol-bio","8":"tag-experiment","9":"tag-qpcr","10":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.8 (Yoast SEO v27.8) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>\u3010\u3084\u3063\u3066\u307f\u305f\u3011\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3000\u30e9\u30f3\u30e2\u30fc\u30c9\u3067\u7d50\u679c\u306b\u5dee\u304c\u3067\u308b\u304b\u6bd4\u8f03\u3057\u3066\u307f\u305f - Learning at the Bench<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr_workshop_gsd_ts_3\/\" \/>\n<meta property=\"og:locale\" content=\"ja_JP\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"\u3010\u3084\u3063\u3066\u307f\u305f\u3011\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3000\u30e9\u30f3\u30e2\u30fc\u30c9\u3067\u7d50\u679c\u306b\u5dee\u304c\u3067\u308b\u304b\u6bd4\u8f03\u3057\u3066\u307f\u305f\" \/>\n<meta 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