{"id":14646,"date":"2023-07-07T13:51:11","date_gmt":"2023-07-07T04:51:11","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/?p=14646"},"modified":"2023-12-27T15:49:00","modified_gmt":"2023-12-27T06:49:00","slug":"workshop_microarray_gsd_ts-2","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/workshop_microarray_gsd_ts-2\/","title":{"rendered":"\u3010\u3084\u3063\u3066\u307f\u305f\u3011\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u306e\u30cf\u30a4\u30d6\u30ea\u6642\u9593\u3092\u5ef6\u9577\u3057\u3066\u307f\u305f"},"content":{"rendered":"

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\u5b9f\u9a13\u65b9\u6cd5<\/h2>\n

Applied Biosystems\u2122 GeneChips\u2122 WT PLUS Reagent kit\u3092\u4f7f\u7528\u3057\u3066\u3001\u30ad\u30c3\u30c8\u306b\u4ed8\u5c5e\u3059\u308bControl HeLa RNA\u3092\u57fa\u306b\u30cf\u30a4\u30d6\u30ea\u30c0\u30a4\u30bc\u30fc\u30b7\u30e7\u30f3\u30ab\u30af\u30c6\u30eb\u3092\u8abf\u88fd\u3057\u307e\u3057\u305f\u3002\u30ab\u30af\u30c6\u30eb\u306e\u8abf\u88fd\u5de5\u7a0b\u306b\u304a\u3051\u308b\u30b5\u30f3\u30d7\u30eb\u9593\u5dee\u3092\u306a\u304f\u3059\u305f\u3081\u306b\u3001\u4e00\u5ea61\u3064\u306b\u6df7\u305c\u5408\u308f\u305b\u3066\u304b\u3089\u5206\u6ce8\u3057\u305f\u3082\u306e\u3092\u4f7f\u7528\u3057\u3066\u3044\u307e\u3059\u3002\u30a2\u30ec\u30a4\u7a2e\u306fApplied Biosystems\u2122 Clariom\u2122 S human array\u3092\u4f7f\u7528\u3057\u307e\u3057\u305f\u3002\u307e\u305f\u3001\u30cf\u30a4\u30d6\u30ea\u30c0\u30a4\u30bc\u30fc\u30b7\u30e7\u30f3\u306e\u6642\u9593\u306f\u3001\u30d7\u30ed\u30c8\u30b3\u30eb\u306b\u8a18\u8f09\u3055\u308c\u3066\u3044\u308b16\u6642\u9593\u3092\u57fa\u6e96\u306b\u3001\u5ef6\u9577\u6642\u306e\u4e0a\u9650\u3068\u3057\u3066\u63a8\u5968\u3057\u3066\u3044\u308b18\u6642\u9593\u3001\u305d\u3057\u3066\u4e381\u65e5\u5b9f\u65bd\u3057\u305f24\u6642\u9593\u3068\u3055\u3089\u306b48\u6642\u9593\u307e\u3067\u5ef6\u9577\u3057\u305f4\u70b9\uff08n=2\uff09\u3067\u8a55\u4fa1\u3057\u307e\u3057\u305f\u3002<\/p>\n

\u5b9f\u9a13\u7d50\u679c\u30fb\u8003\u5bdf<\/h2>\n

Applied Biosystems\u2122 Transcriptome Analysis Console\uff08TAC\uff09\u3067\u89e3\u6790\u3057\u305f\u30c7\u30fc\u30bf\u3092\u4e0b\u8a18\u306b\u304a\u793a\u3057\u3057\u307e\u3059\u300216\u6642\u9593\u306e\u767a\u73fe\u89e3\u6790\u7d50\u679c\u3092\u57fa\u6e96\u306b18\uff5e48\u6642\u9593\u306e\u7d50\u679c\u3068\u6bd4\u8f03\u89e3\u6790\u3057\u3066\u307f\u308b\u3068\u3001\u767a\u73fe\u304c\u5909\u52d5\u3057\u3066\u3044\u308b\u3068\u5224\u5b9a\u3055\u308c\u305f\u907a\u4f1d\u5b50\u306f18\u6642\u9593\u306824\u6642\u9593\u3067\u306f120\uff5e130\u7a2e\u7a0b\u5ea6\u3067\u3059\u304c\u300148\u6642\u9593\u3067\u306f\u7d042\u500d\u306e251\u7a2e\u307e\u3067\u5897\u52a0\u3057\u305f\u3053\u3068\u304c\u308f\u304b\u308a\u307e\u3057\u305f\u3002\u5185\u8a33\u3092\u898b\u3066\u307f\u308b\u3068\u3001Up-Regulated\uff0818\uff5e48\u6642\u9593\u5074\uff09\u306e\u5272\u5408\u304c\u5f90\u3005\u306b\u6e1b\u5c11\u3057\u3001Down-regulated\uff0816\u6642\u9593\u5074\uff09\u306e\u5272\u5408\u304c\u5897\u52a0\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u8aad\u307f\u53d6\u308c\u307e\u3059\u3002\u3064\u307e\u308a\u3001\u30cf\u30a4\u30d6\u30ea\u30c0\u30a4\u30bc\u30fc\u30b7\u30e7\u30f3\u5de5\u7a0b\u3092\u9577\u6642\u9593\u884c\u3046\u3053\u3068\u306b\u3088\u308a\u3001\u767a\u73fe\u91cf\u304c\u4f4e\u4e0b\u3057\u3066\u3044\u308b\u53ef\u80fd\u6027\u304c\u793a\u5506\u3055\u308c\u307e\u3057\u305f\u3002<\/p>\n

\"\u767a\u73fe\u5909\u52d5\u907a\u4f1d\u5b50\u6570\"

\u56f31. \u767a\u73fe\u5909\u52d5\u907a\u4f1d\u5b50\u6570<\/p><\/div>\n

\"\u5404\u30a2\u30ec\u30a4\u306e\u30b7\u30b0\u30ca\u30eb\u5f37\u5ea6\"

\u56f32. \u5404\u30a2\u30ec\u30a4\u306e\u30b7\u30b0\u30ca\u30eb\u5f37\u5ea6<\/p><\/div>\n

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\n\u9577\u6642\u9593\u306e\u30cf\u30a4\u30d6\u30ea\u30c0\u30a4\u30bc\u30fc\u30b7\u30e7\u30f3\u306b\u3088\u308b\u5f71\u97ff\u3068\u3057\u3066\u61f8\u5ff5\u3055\u308c\u308b\u306e\u306f\u3001\u975e\u7279\u7570\u7684\u306a\u7d50\u5408\u306b\u4f34\u3046\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9\u30ce\u30a4\u30ba\u306e\u4e0a\u6607\u3067\u3059\u3002\u4f8b\u3048\u3070\u3001\u9577\u6642\u9593\u9ad8\u6e29\u306e\u72b6\u614b\u304c\u7d9a\u304f\u3053\u3068\u3067\u30cf\u30a4\u30d6\u30ea\u30c0\u30a4\u30bc\u30fc\u30b7\u30e7\u30f3\u30ab\u30af\u30c6\u30eb\u304c\u84b8\u767a\u3057\u3066\u6790\u51fa\u3057\u305f\u6210\u5206\u304c\u30a2\u30ec\u30a4\u4e0a\u306b\u4ed8\u7740\u3059\u308b\u5834\u5408\u3084\u3001\u30ab\u30af\u30c6\u30eb\u304c\u6fc3\u7e2e\u3055\u308c\u308b\u3053\u3068\u3067\u30cf\u30a4\u30d6\u30ea\u30c0\u30a4\u30bc\u30fc\u30b7\u30e7\u30f3\u306e\u52b9\u7387\u304c\u5909\u5316\u3059\u308b\u53ef\u80fd\u6027\u304c\u8003\u3048\u3089\u308c\u307e\u3059\u3002\u5b9f\u969b\u306b\u30c7\u30fc\u30bf\u3092\u898b\u3066\u307f\u308b\u3068\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9\u306e\u30b7\u30b0\u30ca\u30eb\u5024\u306f18\u6642\u9593\u4ee5\u964d\u304b\u3089\u5897\u52a0\u50be\u5411\u306b\u3042\u308b\u3053\u3068\u304c\u308f\u304b\u308a\u307e\u3057\u305f\u3002<\/p>\n

\"\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9\u30b7\u30b0\u30ca\u30eb\u306e\u6bd4\u8f03\"

\u56f33. \u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9\u30b7\u30b0\u30ca\u30eb\u306e\u6bd4\u8f03<\/p><\/div>\n

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\u307e\u3068\u3081<\/h2>\n

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\u95a2\u9023\u30aa\u30f3\u30e9\u30a4\u30f3\u30bb\u30df\u30ca\u30fc<\/h3>\n

\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u306e\u907a\u4f1d\u5b50\u767a\u73fe\u30c7\u30fc\u30bf\u3092\u89e3\u6790\u3057\u3066\u307f\u307e\u3057\u3087\u3046
\n\uff5e\u7121\u511f\u89e3\u6790\u30bd\u30d5\u30c8\u30a6\u30a8\u30a2TAC\u306e\u3054\u7d39\u4ecb\uff5e<\/a><\/p>\n

\u57fa\u790e\u304b\u3089\u308f\u304b\u308b\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u306b\u3088\u308b\u907a\u4f1d\u5b50\u767a\u73fe\u89e3\u6790\u30bb\u30df\u30ca\u30fc
\n\uff5e\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u306e\u539f\u7406\u304b\u3089\u4f7f\u7528\u4f8b\u306e\u3054\u7d39\u4ecb\uff5e<\/a><\/p>\n

\u95a2\u9023\u30cf\u30f3\u30ba\u30aa\u30f3\u30c8\u30ec\u30fc\u30cb\u30f3\u30b0<\/h3>\n

\u4eca\u65e5\u304b\u3089\u59cb\u3081\u308b\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4 \uff1a \u30cf\u30f3\u30ba\u30aa\u30f3\u30c8\u30ec\u30fc\u30cb\u30f3\u30b0
\n\uff5e\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u306e\u539f\u7406\u304b\u3089\u30c7\u30fc\u30bf\u89e3\u6790\u307e\u3067\uff5e<\/a><\/p>\n

 <\/p>\n

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<\/p>\n","protected":false},"author":975,"featured_media":13028,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[156],"tags":[71,28],"division":[],"class_list":{"0":"post-14646","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-mol-bio","8":"tag-microarray","9":"tag-experiment","10":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"\n\u3010\u3084\u3063\u3066\u307f\u305f\u3011\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u306e\u30cf\u30a4\u30d6\u30ea\u6642\u9593\u3092\u5ef6\u9577\u3057\u3066\u307f\u305f 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