{"id":1471,"date":"2018-10-11T00:00:00","date_gmt":"2018-10-10T15:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/cryopreservation_1\/"},"modified":"2025-07-25T16:37:40","modified_gmt":"2025-07-25T07:37:40","slug":"cryopreservation_1","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/cryopreservation_1\/","title":{"rendered":"\u3010\u51cd\u7d50\u4fdd\u5b58\u306e\u57fa\u790e 1\u3011\u30af\u30e9\u30a4\u30aa\u30c1\u30e5\u30fc\u30d6\u9078\u3073\u3067\u5927\u5207\u306a\uff15\u3064\u306e\u3053\u3068"},"content":{"rendered":"\n<p>\u8457\uff1aArne Johansson, Senior Scientist, Thermo Fisher Scientific<\/p>\n\n\n\n<p>\u751f\u7269\u5b66\u7684\u8a66\u6599\u306e\u51cd\u7d50\u4fdd\u5b58\u306b\u306f\u3001\u591a\u304f\u306e\u30e1\u30fc\u30ab\u30fc\u304b\u3089\u8ca9\u58f2\u3055\u308c\u3066\u3044\u308b\u3055\u307e\u3056\u307e\u306a\u7a2e\u985e\u306e\u51cd\u7d50\u4fdd\u5b58\u7528\u30c1\u30e5\u30fc\u30d6\uff0d\u3044\u308f\u3086\u308b\u30af\u30e9\u30a4\u30aa\u30c1\u30e5\u30fc\u30d6\uff0d\u304c\u4f7f\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u51cd\u7d50\u4fdd\u5b58\u306e\u76ee\u7684\u306f\u3001\u751f\u4f53\u8a66\u6599\u3084\u7d30\u80de\u3092\u4fdd\u5b58\u5f8c\u306b\u7814\u7a76\u306a\u3069\u306b\u4f7f\u7528\u3059\u308b\u305f\u3081\u3001\u30b5\u30f3\u30d7\u30eb\u306e\u7279\u6027\u5909\u5316\u3092\u6700\u5c0f\u9650\u306b\u6291\u3048\u3066\u3001\u9577\u671f\u9593\u3001\u5b89\u5168\u306b\u4fdd\u5b58\u3059\u308b\u3053\u3068\u3067\u3059\u3002\u51cd\u7d50\u4fdd\u5b58\u3055\u308c\u308b\u30b5\u30f3\u30d7\u30eb\u306f\u8cb4\u91cd\u306a\u30b5\u30f3\u30d7\u30eb\u3067\u3042\u308b\u3053\u3068\u304c\u591a\u3044\u305f\u3081\u3001\u4fdd\u5b58\u4e2d\u306b\u30b5\u30f3\u30d7\u30eb\u3078\u306e\u306a\u3093\u3089\u304b\u306e\u5f71\u97ff\u3084\u640d\u50b7\u3092\u4e0e\u3048\u308b\u3053\u3068\u304c\u306a\u3044\u30af\u30e9\u30a4\u30aa\u30c1\u30e5\u30fc\u30d6\u3092\u9078\u3076\u3053\u3068\u304c\u6975\u3081\u3066\u91cd\u8981\u3067\u3059\u3002\u7d30\u80de\u57f9\u990a\u306b\u7cbe\u901a\u3057\u305f\u4eba\u304b\u3089\u3082\u898b\u843d\u3068\u3055\u308c\u304c\u3061\u3067\u3059\u304c\u3001\u30af\u30e9\u30a4\u30aa\u30c1\u30e5\u30fc\u30d6\u3092\u9078\u5b9a\u3059\u308b\u3068\u304d\u306f\u3001\u30d5\u30a3\u30c3\u30c8\u611f\u3084\u64cd\u4f5c\u6027\u306a\u3069\u306e\u611f\u899a\u7684\u306a\u7279\u9577\u4ee5\u5916\u306b\u3082\u3001\u76ee\u306b\u898b\u3048\u306a\u3044\u88fd\u54c1\u306e\u54c1\u8cea\u3092\u3088\u304f\u7406\u89e3\u3057\u3066\u3001\u9577\u671f\u304b\u3064\u5b89\u5168\u306b\u4fdd\u5b58\u3067\u304d\u308b\u30c1\u30e5\u30fc\u30d6\u3092\u9078\u3076\u3053\u3068\u304c\u5927\u5207\u3067\u3059\u3002<\/p>\n\n\n\n<p>\u3053\u306e\u30d6\u30ed\u30b0\u3067\u306f\u3001<span style=\"text-decoration: underline\">\u30af\u30e9\u30a4\u30aa\u30c1\u30e5\u30fc\u30d6\u3092\u9078\u3076\u6642\u306b\u91cd\u8996\u3057\u305f\u30445\u3064\u306e\u30dd\u30a4\u30f3\u30c8<\/span>\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\uff11-\u6c17\u5bc6\u6027-tightness\">\uff11\uff0e\u6c17\u5bc6\u6027\uff08Tightness\uff09<\/h2>\n\n\n\n<p>\u30b5\u30f3\u30d7\u30eb\u306e\u9577\u671f\u4fdd\u5b58\uff0d\u7279\u306b\u6db2\u4f53\u7a92\u7d20\u4e2d\u3067\u306e\u4fdd\u5b58\uff0d\u306b\u4f7f\u308f\u308c\u308b\u30af\u30e9\u30a4\u30aa\u30c1\u30e5\u30fc\u30d6\u306b\u306f\u3001\u6db2\u4f53\u7a92\u7d20\u306a\u3069\u306e\u30c1\u30e5\u30fc\u30d6\u5185\u90e8\u3078\u306e\u6d41\u5165\u3084\u30c1\u30e5\u30fc\u30d6\u5916\u90e8\u3078\u306e\u30b5\u30f3\u30d7\u30eb\u306e\u6db2\u6f0f\u308c\u3092\u9632\u6b62\u3059\u308b\u30b7\u30fc\u30ea\u30f3\u30b0\u6750\u304c\u5fc5\u8981\u3067\u3059\u3002\u4fdd\u5b58\u4e2d\u306b\u6db2\u4f53\u7a92\u7d20\u304c\u30af\u30e9\u30a4\u30aa\u30c1\u30e5\u30fc\u30d6\u5185\u306b\u6d41\u5165\u3059\u308b\u3068\u3001\u878d\u89e3\u6642\u306b\u30c1\u30e5\u30fc\u30d6\u5185\u306e\u6db2\u4f53\u7a92\u7d20\u304c\u6025\u901f\u306b\u6c17\u5316\u3057\u3066\u81a8\u5f35\u3057\u30c1\u30e5\u30fc\u30d6\u304c\u7834\u88c2\u3059\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u6db2\u4f53\u7a92\u7d20\u306f\u5ba4\u6e29\u306b\u52a0\u6e29\u3055\u308c\u308b\u3068\u7d04700\u500d\u306b\u81a8\u5f35\u3059\u308b\u305f\u3081\u3067\u3059\u3002\u81a8\u5f35\u304c\u59a8\u3052\u3089\u308c\u308b\u5834\u5408\u3001\u306a\u3093\u3068\u30c1\u30e5\u30fc\u30d6\u5185\u306b\u306f\u7d04700\u6c17\u5727\u3082\u306e\u5727\u529b\u304c\u304b\u304b\u308a\u307e\u3059\uff01\u8d85\u4f4e\u6e29\u4e0b\u3067\u306f\u3001\u3069\u306e\u3088\u3046\u306a\u30b7\u30fc\u30ea\u30f3\u30b0\u6750\u3067\u3082\u67d4\u8edf\u6027\u3092\u5931\u3046\u305f\u3081\u3001O-\u30ea\u30f3\u30b0\u306a\u3069\u306e\u30ac\u30b9\u30b1\u30c3\u30c8\u3084\u30d1\u30c3\u30ad\u30f3\u3092\u4f7f\u3063\u3066\u3044\u308b\u30c1\u30e5\u30fc\u30d6\u306b\u306f\u3001\u7406\u8ad6\u4e0a\u5e38\u306b\u6db2\u6f0f\u308c\u306e\u30ea\u30b9\u30af\u304c\u751f\u3058\u307e\u3059\u3002\u6db2\u4f53\u7a92\u7d20\u4e2d\u3067\u6db2\u76f8\u4fdd\u5b58\u3055\u308c\u3066\u3044\u308b\u30af\u30e9\u30a4\u30aa\u30c1\u30e5\u30fc\u30d6\u3092\u5ba4\u6e29\u306b\u53d6\u308a\u51fa\u3059\u969b\u306b\u306f\u3001\u30c1\u30e5\u30fc\u30d6\u3092\u6db2\u4f53\u7a92\u7d20\u51cd\u7d50\u5bb9\u5668\u5185\u306e\u6c17\u76f8\u306b24\u6642\u9593\u4ee5\u4e0a\u4fdd\u6301\u3057\u305f\u3042\u3068\u3001\u5bb9\u5668\u304b\u3089\u53d6\u308a\u51fa\u3059\u3053\u3068\u3067\u30c1\u30e5\u30fc\u30d6\u306e\u7834\u88c2\u30ea\u30b9\u30af\u3092\u56de\u907f\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u30af\u30e9\u30a4\u30aa\u30c1\u30e5\u30fc\u30d6\u3092\u6c17\u76f8\u306b\u3057\u3070\u3089\u304f\u653e\u7f6e\u3059\u308b\u3053\u3068\u3067\u3001\u30ad\u30e3\u30c3\u30d7\u306e\u9699\u9593\u304b\u3089\u30c1\u30e5\u30fc\u30d6\u5185\u306b\u6d41\u5165\u3057\u305f\u6db2\u4f53\u7a92\u7d20\u3092\u3086\u3063\u304f\u308a\u3068\u84b8\u767a\u3055\u305b\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u304b\u3089\u3067\u3059\u3002\u51cd\u7d50\u4fdd\u5b58\u6642\u306b\u306f\u3001\u8d85\u4f4e\u6e29\u3067\u3082\u8131\u843d\u30ea\u30b9\u30af\u306e\u5c11\u306a\u3044O-\u30ea\u30f3\u30b0\u3001\u30ad\u30e3\u30c3\u30d7\u5185\u84cb\uff08\u30ad\u30e3\u30c3\u30d7\u30e9\u30a4\u30ca\u30fc\uff09\u3084\u6db2\u6f0f\u308c\u3057\u306a\u3044\u69cb\u9020\u3092\u3082\u3064\u30af\u30e9\u30a4\u30aa\u30c1\u30e5\u30fc\u30d6\u3092\u9078\u3076\u3053\u3068\u304c\u5927\u5207\u3067\u3059\u3002\u30af\u30e9\u30a4\u30aa\u30c1\u30e5\u30fc\u30d6\u3078\u306e\u6db2\u4f53\u7a92\u7d20\u306e\u6d41\u5165\u3092100%\u9632\u3050\u305f\u3081\u306b\u306f\u3001\u5916\u90e8\u4fdd\u8b77\u88ab\u8986\u3092\u4f7f\u3063\u3066\u30c1\u30e5\u30fc\u30d6\u3092\u78ba\u5b9f\u306b\u5bc6\u5c01\u3059\u308b\u3053\u3068\u304c\u5fc5\u8981\u3067\u3059\u3002Thermo Scientific <sup>TM<\/sup> Nunc<sup>TM<\/sup> \u30af\u30e9\u30a4\u30aa\u30a2\u30af\u30bb\u30b5\u30ea\u306b\u306f\u3001\u6db2\u76f8\u4fdd\u5b58\u3059\u308b\u30af\u30e9\u30a4\u30aa\u30c1\u30e5\u30fc\u30d6\u3092\u5bc6\u5c01\u3059\u308b\u305f\u3081\u306e\u3001\u30d2\u30fc\u30c8\u30b7\u30fc\u30eb\u53ef\u80fd\u306a\u30dd\u30ea\u30a8\u30c1\u30ec\u30f3\u88fd\u30c1\u30e5\u30fc\u30d6\u3067\u3042\u308b<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/343958PK?CID=lpd_lpe_cls_r04_jp_cp0000_pjt0000_lpd00000_0so_blg_op_awa_oc_s00_tube8_Social_LAB\" target=\"_blank\" rel=\"noopener\"> Nunc<sup>TM<\/sup>CryoFlex<sup>TM<\/sup>\uff08\u88fd\u54c1\u756a\u53f7\uff1a343958\uff09<\/a>\u3092\u3054\u7528\u610f\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\uff12-\u7d30\u80de\u6bd2\u6027\">\uff12\uff0e\u7d30\u80de\u6bd2\u6027<\/h2>\n\n\n\n<p>\u57f9\u990a\u7d30\u80de\u306a\u3069\u306e\u751f\u7269\u5b66\u7684\u8a66\u6599\u3092\u751f\u304d\u305f\u307e\u307e\u3001\u9577\u671f\u306b\u308f\u305f\u308a\u51cd\u7d50\u4fdd\u5b58\u3059\u308b\u305f\u3081\u306b\u306f\u3001\u7d30\u80de\u6bd2\u6027\u3092\u793a\u3055\u306a\u3044\u539f\u6599\u3067\u4f5c\u3089\u308c\u305f\u30af\u30e9\u30a4\u30aa\u30c1\u30e5\u30fc\u30d6\u3092\u4f7f\u3046\u3053\u3068\u304c\u91cd\u8981\u3067\u3059\u3002\u751f\u304d\u305f\u7d30\u80de\u3092\u8d85\u4f4e\u6e29\u3067\u51cd\u7d50\u4fdd\u5b58\u3059\u308b\u305f\u3081\u306b\u3001\u30d7\u30e9\u30b9\u30c1\u30c3\u30af\u88fd\u306e\u30af\u30e9\u30a4\u30aa\u30c1\u30e5\u30fc\u30d6\u304c\u3088\u304f\u4f7f\u7528\u3055\u308c\u307e\u3059\u3002\u3057\u304b\u3057\u3001\u30d7\u30e9\u30b9\u30c1\u30c3\u30af\u539f\u6599\u306e\u54c1\u8cea\u306b\u3088\u3063\u3066\u306f\u7d30\u80de\u306b\u5bfe\u3057\u3066\u6bd2\u6027\u3092\u793a\u3059\u4e0d\u7d14\u7269\u3084\u6dfb\u52a0\u7269\u3092\u542b\u3093\u3067\u3044\u308b\u53ef\u80fd\u6027\u304c\u3042\u308b\u3082\u306e\u3082\u5b58\u5728\u3059\u308b\u305f\u3081\u3001\u30d7\u30e9\u30b9\u30c1\u30c3\u30af\u306e\u6750\u8cea\u30b0\u30ec\u30fc\u30c9\u3092\u7cbe\u67fb\u3059\u308b\u3053\u3068\u306f\u6975\u3081\u3066\u91cd\u8981\u3067\u3059\u3002\u30af\u30e9\u30a4\u30aa\u30c1\u30e5\u30fc\u30d6\u3092\u9078\u3076\u969b\u306b\u306f\u3001\u7d30\u80de\u7121\u6bd2\u6027\u304c\u8a3c\u660e\u3055\u308c\u3066\u3044\u308b\u30d7\u30e9\u30b9\u30c1\u30c3\u30af\u3092\u539f\u6599\u3068\u3057\u305f\u30c1\u30e5\u30fc\u30d6\u3092\u9078\u3076\u3053\u3068\u304c\u975e\u5e38\u306b\u91cd\u8981\u3067\u3059\u3002<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/5000-0020?CID=lpd_lpe_cls_r04_jp_cp0000_pjt0000_lpd00000_0so_blg_op_awa_oc_s00_tube8_Social_LAB\" target=\"_blank\" rel=\"noopener\">Thermo Scientific&nbsp;<sup>TM<\/sup> Nalgene&nbsp;<sup>TM<\/sup>\u30af\u30e9\u30a4\u30aa\u30d0\u30a4\u30a2\u30eb<\/a>\u3068<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/375418PK?CID=lpd_lpe_cls_r04_jp_cp0000_pjt0000_lpd00000_0so_blg_op_awa_oc_s00_tube8_Social_LAB\" target=\"_blank\" rel=\"noopener\">Nunc\u30af\u30e9\u30a4\u30aa\u30c1\u30e5\u30fc\u30d6<\/a>\u306f\u4ee5\u4e0b\u306e\u8a66\u9a13\u306b\u5408\u683c\u3057\u3066\u304a\u308a\u3001\u54fa\u4e73\u52d5\u7269\u7d30\u80de\u306b\u5bfe\u3059\u308b\u7d30\u80de\u6bd2\u6027\u304c\u306a\u3044\u3053\u3068\u304c\u8a3c\u660e\u3055\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li>Growth inhibition test (Ref.: Materials Science Toxicology Laboratories, University of Tennessee, USA)<\/li>\n\n\n\n<li>Cloning inhibition test (Ref.: Thermo Fisher Scientific, 1985)<\/li>\n<\/ol>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\uff13-\u5909\u7570\u539f\u6027-mutagenicity\">\uff13\uff0e\u5909\u7570\u539f\u6027\uff08Mutagenicity<\/h2>\n\n\n\n<p>\u57f9\u990a\u7d30\u80de\u3092\u306f\u3058\u3081\u3068\u3057\u305f\u751f\u7269\u5b66\u7684\u8a66\u6599\u3092\u51cd\u7d50\u4fdd\u5b58\u3059\u308b\u76ee\u7684\u306e\u4e00\u3064\u3068\u3057\u3066\u306f\u3001\u672a\u5909\u7570\u306e\u6a19\u6e96\u682a\u3092\u7dad\u6301\u3059\u308b\u3053\u3068\u304c\u3042\u3052\u3089\u308c\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u3001\u30af\u30e9\u30a4\u30aa\u30c1\u30e5\u30fc\u30d6\u304b\u3089\u6eb6\u51fa\u306e\u53ef\u80fd\u6027\u304c\u3042\u308b\u5316\u5b66\u7269\u8cea\u306b\u3088\u3063\u3066\u3001\u751f\u7269\u306e\u907a\u4f1d\u60c5\u5831\uff08DNA\u3042\u308b\u3044\u306f\u67d3\u8272\u4f53\uff09\u306b\u7a81\u7136\u5909\u7570\u3092\u8a98\u767a\u3055\u305b\u308b\u3088\u3046\u306a\u5909\u7570\u539f\u6027\u304c\u306a\u3044\u3068\u3055\u308c\u3066\u3044\u308b\u30c1\u30e5\u30fc\u30d6\u3092\u4f7f\u3046\u3053\u3068\u306f\u6975\u3081\u3066\u91cd\u8981\u3067\u3059\u3002\u4fdd\u5b58\u4e2d\u306b\u7d30\u80de\u306b\u5438\u53ce\u3055\u308c\u305f\u5316\u5b66\u7269\u8cea\u306e\u5f71\u97ff\u306f\u3001\u51cd\u7d50\u4fdd\u5b58\u4e2d\u306b\u505c\u6b62\u3057\u3066\u3044\u308b\u751f\u547d\u73fe\u8c61\u306b\u9577\u671f\u7684\u306a\u5f71\u97ff\u3092\u304a\u3088\u307c\u3059\u305f\u3081\u3001\u4fdd\u5b58\u5f8c\u306b\u591a\u6570\u306e\u7d30\u80de\u5206\u88c2\u3092\u7d4c\u3066\u521d\u3081\u3066\u7570\u306a\u308b\u5f62\u8cea\u304c\u767a\u73fe\u3059\u308b\u53ef\u80fd\u6027\u304c\u3042\u308b\u3068\u3044\u3046\u3053\u3068\u306b\u7559\u610f\u3057\u306a\u3051\u308c\u3070\u306a\u308a\u307e\u305b\u3093\u3002\u5909\u7570\u539f\u6027\u306e\u5f71\u97ff\u306f\u3001\u51cd\u7d50\u878d\u89e3\u5f8c\u3001\u7d30\u80de\u3092\u8907\u6570\u56de\u7d99\u4ee3\u57f9\u990a\u3059\u308b\u307e\u3067\u73fe\u308c\u306a\u3044\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u3042\u3068\u3042\u3068\u5b9f\u65bd\u3057\u305f\u691c\u67fb\u306b\u3088\u3063\u3066\u3001\u4f7f\u3063\u3066\u3044\u305f\u7d30\u80de\u304c\u5909\u7570\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u5224\u660e\u3059\u308b\u3068\u3001\u5b9f\u9a13\u306e\u3084\u308a\u76f4\u3057\u306b\u7e4b\u304c\u308a\u304b\u306d\u307e\u305b\u3093\u3002\u5909\u7570\u539f\u6027\u8a66\u9a13\u306b\u5408\u683c\u3057\u305f\u30af\u30e9\u30a4\u30aa\u30c1\u30e5\u30fc\u30d6\u3092\u9078\u3076\u3053\u3068\u306f\u3068\u3066\u3082\u5927\u5207\u3067\u3059\u3002Nunc\u30af\u30e9\u30a4\u30aa\u30c1\u30e5\u30fc\u30d6\u306f\u30a8\u30fc\u30e0\u30ba\u8a66\u9a13\uff08\u5316\u5b66\u7269\u8cea\u306e\u5909\u7570\u539f\u6027\u3092\u8a55\u4fa1\u3059\u308b\u305f\u3081\u306e\u8a66\u9a13\u65b9\u6cd5\uff09\u306b\u5408\u683c\u3057\u3066\u304a\u308a\u3001\u5909\u7570\u539f\u6027\u304c\u8a8d\u3081\u3089\u308c\u306a\u3044\u3053\u3068\u304c\u8a3c\u660e\u3055\u308c\u3066\u3044\u307e\u3059\u3002\uff08\u53c2\u7167\uff1aOECD Salmonella typhimurium, Reverse Mutation Assay No. 471, 1983\uff09<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\uff14-\u767a\u71b1\u6027-pyrogenicity\">\uff14\uff0e\u767a\u71b1\u6027\uff08Pyrogenicity\uff09<\/h2>\n\n\n\n<p>\u30af\u30e9\u30a4\u30aa\u30c1\u30e5\u30fc\u30d6\u306f\u3001\u85ac\u7406\u5b66\u8a66\u9a13\u3067\u4f7f\u7528\u3055\u308c\u308b\u6d3b\u6027\u7269\u8cea\u306e\u4fdd\u5b58\u306b\u4f7f\u308f\u308c\u308b\u3053\u3068\u3082\u3042\u308a\u307e\u3059\u3002\u767a\u71b1\u6027\u306e\u4e0d\u7d14\u7269\u306f\u3001\u85ac\u7406\u5b66\u7684\u6d3b\u6027\u7269\u8cea\u306e\u52b9\u679c\u3092\u963b\u5bb3\u3059\u308b\u53ef\u80fd\u6027\u304c\u3042\u308b\u305f\u3081\u3001\u30d1\u30a4\u30ed\u30b8\u30a7\u30f3\u3068\u547c\u3070\u308c\u308b\u767a\u71b1\u7269\u8cea\u306e\u30af\u30e9\u30a4\u30aa\u30c1\u30e5\u30fc\u30d6\u304b\u3089\u306e\u691c\u51fa\u306f\u8a31\u5bb9\u3055\u308c\u307e\u305b\u3093\u3002\u4ee3\u8868\u7684\u306a\u767a\u71b1\u7269\u8cea\u306e\u3072\u3068\u3064\u3067\u3042\u308b\u30a8\u30f3\u30c9\u30c8\u30ad\u30b7\u30f3\u306f\u3001\u30af\u30e9\u30a4\u30aa\u30c1\u30e5\u30fc\u30d6\u306b\u4f7f\u7528\u3055\u308c\u308b\u30dd\u30ea\u30d7\u30ed\u30d4\u30ec\u30f3\u306b\u5bfe\u3057\u3066\u7279\u306b\u5438\u7740\u6027\u304c\u9ad8\u3044\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u3044\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u3001\u30af\u30e9\u30a4\u30aa\u30c1\u30e5\u30fc\u30d6\u306b\u6df7\u5165\u3059\u308b\u30a8\u30f3\u30c9\u30c8\u30ad\u30b7\u30f3\u306b\u306f\u7279\u306b\u6ce8\u610f\u3092\u6255\u3046\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002Nunc\u30af\u30e9\u30a4\u30aa\u30c1\u30e5\u30fc\u30d6\u306f\u3001FDA\u30ac\u30a4\u30c9\u30e9\u30a4\u30f3\u306b\u8a18\u8f09\u3055\u308c\u3066\u3044\u308bLimulus Amebocyte Lysate\uff08LAL\uff09\u8a66\u9a13\u306b\u5408\u683c\u3057\u3066\u304a\u308a\u3001\u5b9f\u8cea\u4e0a\u3001\u30a8\u30f3\u30c9\u30c8\u30ad\u30b7\u30f3\u30d5\u30ea\u30fc\u3067\u3042\u308b\u3053\u3068\u304c\u8a3c\u660e\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u3055\u3089\u306b\u3001\u30a8\u30f3\u30c9\u30c8\u30ad\u30b7\u30f3\u30ec\u30d9\u30eb\u306f\u7c73\u56fd\u85ac\u5c40\u65b9\uff08USP\uff1aUnited States Pharmacopeia\uff09\u306b\u8a18\u8f09\u3055\u308c\u3066\u3044\u308b20 EU \/device\uff080.5 EU \/mL\uff09\u672a\u6e80\u3067\u3042\u308b\u3053\u3068\u304c\u6587\u66f8\u5316\u3055\u308c\u3066\u304a\u308a\u3001\u30d1\u30a4\u30ed\u30b8\u30a7\u30f3\u30d5\u30ea\u30fc\uff08\u975e\u767a\u71b1\u6027\uff09\u304c\u4fdd\u8a3c\u3055\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\uff15-\u6ec5\u83cc\u6027-sterility\">\uff15\uff0e\u6ec5\u83cc\u6027\uff08Sterility\uff09<\/h2>\n\n\n\n<p>\u4fdd\u5b58\u671f\u9593\u4e2d\u30b5\u30f3\u30d7\u30eb\u306e\u72b6\u614b\u3092\u5909\u5316\u3055\u305b\u305a\u3001\u9577\u671f\u306b\u308f\u305f\u308a\u5b8c\u5168\u6027\u3092\u7dad\u6301\u3059\u308b\u305f\u3081\u306b\u306f\u3001\u30af\u30e9\u30a4\u30aa\u30c1\u30e5\u30fc\u30d6\u5916\u90e8\u304b\u3089\u306e\u5fae\u751f\u7269\u306b\u3088\u308b\u30b3\u30f3\u30bf\u30df\u30cd\u30fc\u30b7\u30e7\u30f3\u3092\u306a\u304f\u3059\u3053\u3068\u304c\u5fc5\u8981\u3067\u3059\u3002\u7121\u83cc\u6027\u4fdd\u8a3c\u6c34\u6e96\uff08SAL\uff1aSterility Assurance Level\uff09\u304c\u8a3c\u660e\u3055\u308c\u3066\u3044\u308b\u30af\u30e9\u30a4\u30aa\u30c1\u30e5\u30fc\u30d6\u3092\u9078\u3076\u3053\u3068\u306f\u3001\u51cd\u7d50\u4fdd\u5b58\u3067\u4f7f\u7528\u3059\u308b\u30c1\u30e5\u30fc\u30d6\u306e\u91cd\u8981\u306a\u9078\u5b9a\u57fa\u6e96\u3068\u306a\u308a\u307e\u3059\u3002\u7121\u83cc\u6027\u4fdd\u8a3c\u6c34\u6e96\u306f\u9069\u5207\u306a\u6ec5\u83cc\u5de5\u7a0b\u3092\u7d4c\u3066\u6ec5\u83cc\u51e6\u7406\u3055\u308c\u305f\u88fd\u54c1\u4e2d\u306b\u5b58\u5728\u3059\u308b\u83cc\u306e\u6700\u5927\u751f\u5b58\u78ba\u7387\u3092\u3044\u3044\u300110<sup>-n<\/sup>\u3067\u8868\u3055\u308c\u307e\u3059\u3002Nalgene\u30af\u30e9\u30a4\u30aa\u30d0\u30a4\u30a2\u30eb\u3084Nunc\u30af\u30e9\u30a4\u30aa\u30c1\u30e5\u30fc\u30d6\u306b\u306f\u3001\u30d0\u30a4\u30aa\u30d0\u30fc\u30c7\u30f3\u306e\u30e2\u30cb\u30bf\u30ea\u30f3\u30b0\u3068\u305d\u308c\u306b\u7d9a\u304f\u653e\u5c04\u7dda\u6ec5\u83cc\u304c\u884c\u308f\u308c\u3066\u304a\u308a\u3001SAL10<sup>-6<\/sup>\u3092\u9054\u6210\u3059\u308b\u3053\u3068\u3067\u7121\u83cc\u6027\u3092\u4fdd\u8a3c\u3057\u3066\u3044\u307e\u3059\u3002\u3053\u308c\u306f\u3001\u7d71\u8a08\u5b66\u7684\u306b100\u4e07\u672c\u306e\u30c1\u30e5\u30fc\u30d6\u5f53\u305f\u308a\u306e\u6c5a\u67d3\u83cc\u306e\u6700\u5927\u751f\u5b58\u6570\u306f1\u672c\u4ee5\u4e0b\u306b\u76f8\u5f53\u3057\u307e\u3059\u3002<\/p>\n\n\n\n<div class=\"wp-block-group is-layout-constrained wp-block-group-is-layout-constrained\">\n<h4 class=\"wp-block-heading\" id=\"h-\u5f53\u793e\u306e\u51cd\u7d50\u4fdd\u5b58\u30c1\u30e5\u30fc\u30d6\u3092-\u307e\u305a\u306f\u30b5\u30f3\u30d7\u30eb\u3067\u304a\u8a66\u3057\u304f\u3060\u3055\u3044\">\u5f53\u793e\u306e\u51cd\u7d50\u4fdd\u5b58\u30c1\u30e5\u30fc\u30d6\u3092\u3001\u307e\u305a\u306f\u30b5\u30f3\u30d7\u30eb\u3067\u304a\u8a66\u3057\u304f\u3060\u3055\u3044\u3002<\/h4>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/life-science\/cryopreservation-tube-sample.html?cid=lpd_lpe_cls_r04_jp_cp0000_pjt0000_lpd00000_0so_blg_op_awa_oc_s00_tube8_Social_LAB\" target=\"_blank\" rel=\"noopener\" data-wplink-edit=\"true\">\u30b5\u30f3\u30d7\u30eb\u3092\u7533\u3057\u8fbc\u3080<\/a><\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-group is-layout-constrained wp-block-group-is-layout-constrained\">\n<h4 class=\"wp-block-heading\" id=\"h-\u30c1\u30e5\u30fc\u30d6-\u30d7\u30ec\u30fc\u30c8-\u51cd\u7d50\u4fdd\u5b58\u7528\u88fd\u54c1\u30ab\u30bf\u30ed\u30b0\">\u30c1\u30e5\u30fc\u30d6\uff06\u30d7\u30ec\u30fc\u30c8 &#8211; \u51cd\u7d50\u4fdd\u5b58\u7528\u88fd\u54c1\u30ab\u30bf\u30ed\u30b0<\/h4>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><a class=\"btn btn-red\" href=\"https:\/\/assets.thermofisher.com\/TFS-Assets\/LPD\/Scientific-Resources\/sample-storage-products-catalog-ja.pdf?cid=lpd_lpe_cls_r04_jp_cp0000_pjt0000_lpd00000_0so_blg_op_awa_oc_s00_tube8_Social_LAB\" target=\"_blank\" rel=\"noopener\" data-wplink-edit=\"true\">\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u306f\u3053\u3061\u3089<\/a><\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-group is-layout-constrained wp-block-group-is-layout-constrained\">\n<h4 class=\"wp-block-heading\" id=\"h-gibco\u7d30\u80de\u57f9\u990a\u30cf\u30f3\u30c9\u30d6\u30c3\u30af\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\">Gibco\u7d30\u80de\u57f9\u990a\u30cf\u30f3\u30c9\u30d6\u30c3\u30af\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9<\/h4>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/gibco-cell-culture-basics-handbook-form.html?cid=lpd_lpe_cls_r04_jp_cp0000_pjt0000_lpd00000_0so_blg_op_awa_oc_s00_tube8_Social_LAB\" target=\"_blank\" rel=\"noopener\" data-wplink-edit=\"true\">\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u306f\u3053\u3061\u3089<\/a><\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-group is-layout-constrained wp-block-group-is-layout-constrained\">\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>\uff1c\u8cc7\u6599\u8acb\u6c42\u30fb\u304a\u554f\u3044\u5408\u308f\u305b\u306f<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/demo-2d-tube.html?cid=lpd_lpe_cls_r04_jp_cp0000_pjt0000_lpd00000_0so_blg_op_awa_oc_s00_tube8_Social_LAB\" target=\"_blank\" rel=\"noopener\" data-wplink-edit=\"true\">\u3053\u3061\u3089<\/a>\u304b\u3089\uff1e<\/p>\n<\/div>\n\n\n\n<p>&nbsp;<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u95a2\u9023\u60c5\u5831\"><b>\u95a2\u9023\u60c5\u5831<\/b><\/h2>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u53c2\u8003\u8cc7\u6599\uff1a<a href=\"https:\/\/www.thermofisher.com.au\/Uploads\/file\/Scientific\/Applications\/Lab-Plasticware-Glassware-Supplies\/Aspects-of-Cryo-Preservation.PDF\">Thermo Fisher Scientific Technical Bulletin: 03b (TILSPNUNCBU03B 0410)<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/sample-storage-management.html?CID=lpd_lpe_cls_r04_jp_cp0000_pjt0000_lpd00000_0so_blg_op_awa_oc_s00_tube8_Social_LAB\" target=\"_blank\" rel=\"noopener\">Thermo 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id=\"h-\u51cd\u7d50\u4fdd\u5b58\u306e\u57fa\u790e\u30b7\u30ea\u30fc\u30ba-\u305d\u306e\u4ed6\u306e\u8a18\u4e8b\u306f\u3053\u3061\u3089\">\u51cd\u7d50\u4fdd\u5b58\u306e\u57fa\u790e\u30b7\u30ea\u30fc\u30ba\u3001\u305d\u306e\u4ed6\u306e\u8a18\u4e8b\u306f\u3053\u3061\u3089<\/h2>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><tbody><tr><td>\n<ul>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/cryopreservation_2\/?CID=lpd_lpe_cls_r04_jp_cp0000_pjt0000_lpd00000_0so_blg_op_awa_oc_s00_tube8_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u51cd\u7d50\u4fdd\u5b58\u306e\u57fa\u790e 2\u3011\u30b5\u30f3\u30d7\u30eb\u3092\u6db2\u4f53\u7a92\u7d20\u6e29\u5ea6\u4e0b\u3067\u5b89\u5168\u306b\u4fdd\u5b58\u3059\u308b\u79d8\u8a23<\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/cryopreservation_3\/?CID=lpd_lpe_cls_r04_jp_cp0000_pjt0000_lpd00000_0so_blg_op_awa_oc_s00_tube8_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u51cd\u7d50\u4fdd\u5b58\u306e\u57fa\u790e 3\u3011\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\uff01\u51cd\u7d50\u4fdd\u8b77\u5264\u306e\u8c46\u77e5\u8b58<\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/cryopreservation_4\/?CID=lpd_lpe_cls_r04_jp_cp0000_pjt0000_lpd00000_0so_blg_op_awa_oc_s00_tube8_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u51cd\u7d50\u4fdd\u5b58\u306e\u57fa\u790e 4\u3011\u51cd\u7d50\u4fdd\u5b58\u306e\u6e29\u5ea6\u306b\u3064\u3044\u3066<\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/cryopreservation_5\/?CID=lpd_lpe_cls_r04_jp_cp0000_pjt0000_lpd00000_0so_blg_op_awa_oc_s00_tube8_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u51cd\u7d50\u4fdd\u5b58\u306e\u57fa\u790e 5\u3011\u3053\u308c\u3067\u30ab\u30f3\u30da\u30ad\uff01\u751f\u7269\u8a66\u6599\u306e\u56de\u53ce\u3068\u8abf\u6574\u6cd5<\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/cryopreservation_6\/?CID=lpd_lpe_cls_r04_jp_cp0000_pjt0000_lpd00000_0so_blg_op_awa_oc_s00_tube8_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u51cd\u7d50\u4fdd\u5b58\u306e\u57fa\u790e 6\u3011\u30b7\u30fc\u30c9\u30ed\u30c3\u30c8\u30b7\u30b9\u30c6\u30e0\u3063\u3066\u77e5\u3063\u3066\u3044\u307e\u3059\u304b\uff1f<\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/cryopreservation_7\/?CID=lpd_lpe_cls_r04_jp_cp0000_pjt0000_lpd00000_0so_blg_op_awa_oc_s00_tube8_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u51cd\u7d50\u4fdd\u5b58\u306e\u57fa\u790e 7\u3011\u7d30\u80de\u306e\u51cd\u7d50\u51b7\u5374\u901f\u5ea6\u306e\u3072\u307f\u3064<\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/cryopreservation_8-2\/?CID=lpd_lpe_cls_r04_jp_cp0000_pjt0000_lpd00000_0so_blg_op_awa_oc_s00_tube8_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u51cd\u7d50\u4fdd\u5b58\u306e\u57fa\u790e 8\u3011\u51cd\u7d50\u4fdd\u5b58\u306b\u9069\u3057\u305f\u4fdd\u5b58\u5bb9\u5668\u3068\u306f\uff1f<\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/freeze-preservation-laboratory-9-lpd-24070\/?CID=lpd_lpe_cls_r04_jp_cp0000_pjt0000_lpd00000_0so_blg_op_awa_oc_s00_tube8_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u51cd\u7d50\u4fdd\u5b58\u306e\u57fa\u790e 9\u3011\u30ac\u30b9\u30b1\u30c3\u30c8\u306e\u91cd\u8981\u6027<\/a><\/li>\n<\/ul>\n<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p>&nbsp;<\/p>\n\n\n\n<p>\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u76ee\u7684\u304a\u3088\u3073\u305d\u306e\u624b\u7d9a\u304d\u4e0a\u3067\u306e\u4f7f\u7528\u306f\u3067\u304d\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u8457\uff1aArne Johansson, Senior Scientist, Thermo Fisher Scientific 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