{"id":1474,"date":"2018-09-30T00:00:00","date_gmt":"2018-09-29T15:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/user_voice_41_5_superclonal\/"},"modified":"2023-12-27T18:09:03","modified_gmt":"2023-12-27T09:09:03","slug":"user_voice_41_5_superclonal","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/user_voice_41_5_superclonal\/","title":{"rendered":"\u80da\u767a\u751f\u306b\u91cd\u8981\u306a\u5f79\u5272\u3092\u62c5\u3046\u907a\u4f1d\u5b50\u3092\u907a\u4f1d\u5b50\u6539\u5909\u30de\u30a6\u30b9\u3067\u89e3\u6790\uff08\u7b51\u6ce2\u5927\u5b66\u5927\u5b66\u9662 Dinh Thi Huong Tra\u6c0f\u3001\u7b51\u6ce2\u5927\u5b66\u751f\u547d\u79d1\u5b66\u52d5\u7269\u8cc7\u6e90\u30bb\u30f3\u30bf\u30fc \u6c34\u91ce\u8056\u54c9\u6c0f\uff09-\u300cNEXT\u300d2017\u5e747\u6708\u53f7\u63b2\u8f09"},"content":{"rendered":"<h2 style=\"text-align: left\"><span style=\"font-size: 12pt\">\u80da\u767a\u751f\u306b\u91cd\u8981\u306a\u5f79\u5272\u3092\u62c5\u3046\u907a\u4f1d\u5b50\u3092\u907a\u4f1d\u5b50\u6539\u5909\u30de\u30a6\u30b9\u3067\u89e3\u6790<br \/>\n<\/span><\/h2>\n<p><strong>Dinh Thi Huong Tra \u6c0f\uff08 \u7b51\u6ce2\u5927\u5b66\u5927\u5b66\u9662\u535a\u58eb\u8ab2\u7a0b\uff09<\/strong><br \/>\n<strong>\u6c34\u91ce\u8056\u54c9 \u6c0f\uff08 \u7b51\u6ce2\u5927\u5b66\u533b\u5b66\u533b\u7642\u7cfb\u30fb\u751f\u547d\u79d1\u5b66\u52d5\u7269\u8cc7\u6e90\u30bb\u30f3\u30bf\u30fc\u52a9\u6559\uff09<\/strong><\/p>\n<p><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/66c19942ab4ba346fdb64ccc04cde373-17-1.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignleft size-full wp-image-4740\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/66c19942ab4ba346fdb64ccc04cde373-17-1.jpg\" alt=\"Dinh Thi Huong Tra \u6c0f\uff08 \u7b51\u6ce2\u5927\u5b66\u5927\u5b66\u9662\u535a\u58eb\u8ab2\u7a0b\u3001\u5199\u771f\u53f3\uff09\" width=\"250\" height=\"237\" \/><\/a>\u767a\u751f\u751f\u7269\u5b66\u306b\u8208\u5473\u3092\u6301\u3061\u3001\u7b51\u6ce2\u5927\u5b66\u306b\u7559\u5b66\u4e2d\u306eDinh\u6c0f\u3002\u300c\u80da\u767a\u751f\u306b\u95a2\u308f\u308b\u91cd\u8981\u306a\u907a\u4f1d\u5b50\u306b\u3064\u3044\u3066\u7814\u7a76\u3057\u3066\u3044\u307e\u3059\u304c\u3001\u767a\u73fe\u91cf\u304c\u975e\u5e38\u306b\u4f4e\u3044\u306e\u3067\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u691c\u51fa\u306b\u306f\u7279\u7570\u6027\u304c\u9ad8\u3044Superclonal\u4e8c\u6b21\u6297\u4f53\u304c\u6b20\u304b\u305b\u307e\u305b\u3093\u300d\u3068\u8a71\u3057\u307e\u3059\u3002\u5b9f\u9a13\u306b\u306f\u3001\u76ee\u7684\u306b\u5408\u308f\u305b\u3066\u69d8\u3005\u306a\u907a\u4f1d\u5b50\u6539\u5909\u30de\u30a6\u30b9\u3092\u4f7f\u3044\u307e\u3059\u3002\u30b2\u30ce\u30e0\u7de8\u96c6\u30c4\u30fc\u30eb\u3001CRISPR\/Cas9\u3092\u5229\u7528\u3057\u305f\u907a\u4f1d\u5b50\u6539\u5909\u30de\u30a6\u30b9\u4f5c\u88fd\u6cd5\u6280\u8853\u306e\u958b\u767a\u3092\u9032\u3081\u3001Dinh\u6c0f\u306e\u5171\u540c\u7814\u7a76\u8005\u3067\u3082\u3042\u308b\u6c34\u91ce\u8056\u54c9\u6c0f\u3068\u5171\u306b\u304a\u8a71\u3092\u4f3a\u3044\u307e\u3057\u305f\u3002<\/p>\n<h2><span style=\"font-size: 12pt\">\u5185\u5728\u6027\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u3092\u30ce\u30c3\u30af\u30a4\u30f3\u30de\u30a6\u30b9\u3067\u8ffd\u8de1<\/span><\/h2>\n<p>\u300c\u3053\u306e\u907a\u4f1d\u5b50\u306e\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u30de\u30a6\u30b9\u306f\u767a\u751f\u306e\u521d\u671f\u6bb5\u968e\u3067\u81f4\u6b7b\u3068\u306a\u308b\u305f\u3081\u3001\u6a5f\u80fd\u89e3\u6790\u306b\u306f\u5185\u5728\u6027\u907a\u4f1d\u5b50\u306e\u8fd1\u508d\u306b\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u3084FLAG\u914d\u5217\u3092CRISPR\/Cas9\u3067\u30ce\u30c3\u30af\u30a4\u30f3\u3057\u305f\u30de\u30a6\u30b9\u3092\u4f7f\u3044\u307e\u3059\u3002FLAG\u914d\u5217\u306f\u77ed\u3044\u306e\u3067\u672c\u6765\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u6a5f\u80fd\u306b\u5f71\u97ff\u3092\u4e0e\u3048\u305a\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u5bfe\u3059\u308b\u7279\u7570\u7684\u306a\u6297\u4f53\u304c\u306a\u304f\u3066\u3082\u3001\u5e02\u8ca9\u306e\u6297FLAG\u6297\u4f53\u3092\u4f7f\u3048\u308b\u5229\u70b9\u304c\u3042\u308a\u307e\u3059\u300d\u3068Dinh\u6c0f\u3002\u300c\u305f\u3060\u3057\u6211\u3005\u304c\u30bf\u30fc\u30b2\u30c3\u30c8\u306b\u3057\u3066\u3044\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u767a\u73fe\u91cf\u304c\u3068\u3066\u3082\u4f4e\u3044\u306e\u3067\u3001\u4e00\u822c\u7684\u306aAlexa\u6a19\u8b58\u306e\u4e8c\u6b21\u6297\u4f53\u3067\u306f\u691c\u51fa\u3067\u304d\u306a\u3044\u3053\u3068\u304c\u554f\u984c\u3067\u3057\u305f\u3002\u305d\u3053\u30672\u5e74\u524d\u3001\u767a\u58f2\u76f4\u5f8c\u306eSuperclonal\u4e8c\u6b21\u6297\u4f53\u3092\u8a66\u3057\u306b\u4f7f\u3063\u305f\u3068\u3053\u308d\u3001\u9ad8\u3044\u7279\u7570\u6027\u3067\u30bf\u30fc\u30b2\u30c3\u30c8\u3092\u691c\u51fa\u3067\u304d\u308b\u3053\u3068\u304c\u308f\u304b\u308a\u307e\u3057\u305f\u300d\u3068\u56f3\u3092\u793a\u3057\u306a\u304c\u3089\u8aac\u660e\u3057\u307e\u3059\u3002<\/p>\n<h2><span style=\"font-size: 12pt\">\u3055\u3089\u306b\u89e3\u6790\u3092\u9032\u3081\u308b\u305f\u3081\u306b<\/span><\/h2>\n<p>\u300c\u80da\u306e\u767a\u751f\u306b\u91cd\u8981\u306a\u6a5f\u80fd\u3092\u6301\u3064\u907a\u4f1d\u5b50\u3092\u6b20\u640d\u3055\u305b\u305f\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u30de\u30a6\u30b9\u306f\u3001\u51fa\u751f\u524d\u306b\u81f4\u6b7b\u3057\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u5358\u7d14\u306a\u30de\u30c3\u30af\u30a2\u30a6\u30c8\u30de\u30a6\u30b9\u3092\u7528\u3044\u305f\u89e3\u6790\u3067\u306f\u3001\u305d\u306e\u3088\u3046\u306a\u907a\u4f1d\u5b50\u306e\u751f\u4f53\u306b\u304a\u3051\u308b\u6a5f\u80fd\u3092\u660e\u3089\u304b\u306b\u3067\u304d\u307e\u305b\u3093\u3002\u305d\u3053\u3067\u79c1\u305f\u3061\u306f\u3001\u69d8\u3005\u306a\u30ce\u30c3\u30af\u30a4\u30f3\u30de\u30a6\u30b9\u306e\u4f5c\u88fd\u652f\u63f4\u3092\u901a\u3057\u3066\u5171\u540c\u7814\u7a76\u8005\u306eDinh\u6c0f\u3092\u30b5\u30dd\u30fc\u30c8\u3057\u3066\u3044\u307e\u3059\u300d\u3068\u6c34\u91ce\u6c0f\u306f\u8a9e\u308a\u307e\u3059\u3002\u30d9\u30c8\u30ca\u30e0\u306e\u5927\u5b66\u3092\u5352\u696d\u5f8c\u3001\u7b51\u6ce2\u5927\u5b66\u306e\u535a\u58eb\u904e\u7a0b\u6559\u80b2\u30ea\u30fc\u30c7\u30a3\u30f3\u30b0\u30d7\u30ed\u30b0\u30e9\u30e0\uff08\u30d2\u30e5\u30fc\u30de\u30f3\u30d0\u30a4\u30aa\u30ed\u30b8\u30fc\u5b66\u4f4d\u30d7\u30ed\u30b0\u30e9\u30e0\uff09\u3092\u5229\u7528\u3057\u3066\u6765\u65e5\u3057\u305fDinh\u6c0f\u3002\u3053\u308c\u307e\u3067\u306e\u7814\u7a76\u3092\u8ad6\u6587\u306b\u307e\u3068\u3081\u6295\u7a3f\u3059\u308b\u4e88\u5b9a\u3067\u3059\u3002\u6765\u6708\u306b\u306f\u535a\u58eb\u904e\u7a0b\u3092\u4fee\u4e86\u3057\u307e\u3059\u304c\u3001\u305d\u306e\u5f8c\u3082\u7814\u7a76\u5ba4\u306b\u6b8b\u308a\u3001\u6c34\u91ce\u6c0f\u3089\u3068\u958b\u767a\u3057\u305f\u6761\u4ef6\u4ed8\u304d\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u30de\u30a6\u30b9\u306a\u3069\u3092\u4f7f\u3044\u3001\u3055\u3089\u306b\u3053\u306e\u907a\u4f1d\u5b50\u306e\u5f79\u5272\u3092\u6df1\u304f\u7406\u89e3\u3059\u308b\u7814\u7a76\u3092\u9032\u3081\u3066\u3044\u304d\u307e\u3059\u3002<\/p>\n<p><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/552eb4aed1593e952e7a12c7b8a0e9e1-1.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4741 alignnone\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/552eb4aed1593e952e7a12c7b8a0e9e1-1.jpg\" alt=\"Superclonal\u4e8c\u6b21\u6297\u4f53\u3068\u4e00\u822c\u7684\u306aAlexa\u4e8c\u6b21\u6297\u4f53\u306e\u6bd4\u8f03\" width=\"570\" height=\"329\" \/><\/a><span style=\"font-size: 9pt\"><strong>[\u56f3\uff1a Superclonal\u4e8c\u6b21\u6297\u4f53\u3068\u4e00\u822c\u7684\u306aAlexa\u4e8c\u6b21\u6297\u4f53\u306e\u6bd4\u8f03]<\/strong><\/span><br \/>\n<span style=\"font-size: 9pt\">HEK293\u7d30\u80de\u306bFLAG\u30bf\u30b0\u3092\u4ed8\u3051\u305f\u30bf\u30fc\u30b2\u30c3\u30c8\u907a\u4f1d\u5b50\u3092\u5c0e\u5165\u5f8c\u3001\u86cd\u5149\u67d3\u8272\u3002\u4e0a\u6bb5\u306f\u901a\u5e38\u306e\u4e8c\u6b21\u6297\u4f53\u3001\u4e0b\u6bb5\u306fSuperclonal\u4e8c\u6b21\u6297\u4f53\u3092\u4f7f\u7528\u3002\u5de6\u306fDAPI\u3067\u6838\u3001\u771f\u3093\u4e2d\u306f\u6297FLAG\u6297\u4f53\u4e00\u6b21\u6297\u4f53\u3067\u30bf\u30fc\u30b2\u30c3\u30c8\u3092\u67d3\u8272\u3002\u53f3\u306f\u30de\u30fc\u30b8\u753b\u50cf\u3002\u901a\u5e38\u306e\u4e8c\u6b21\u6297\u4f53\u3067\u306f\u975e\u7279\u7570\u7684\u306a\u30b7\u30b0\u30ca\u30eb\u3057\u304b\u691c\u51fa\u3055\u308c\u307e\u305b\u3093\u3067\u3057\u305f\u304c\u3001Superclonal\u4e8c\u6b21\u6297\u4f53\u3067\u306f\u30bf\u30fc\u30b2\u30c3\u30c8\u3092\u7279\u7570\u7684\u306b\u67d3\u8272\u3067\u304d\u307e\u3057\u305f\u3002<\/span><\/p>\n<h2><span style=\"font-size: 12pt\">\u3044\u3044\u3068\u3053\u53d6\u308a\u306e&#8221;\u6b21\u4e16\u4ee3&#8221;\u4e8c\u6b21\u6297\u4f53!<br \/>\nSuperclonal \u4e8c\u6b21\u6297\u4f53<\/span><\/h2>\n<p>Invitrogen\u2122 Alexa Fluor\u2122 Superclonal\u2122\u4e8c\u6b21\u6297\u4f53\u306f\u3001\u300c\u30e2\u30ce\u30af\u30ed\u30fc\u30ca\u30eb\u6297\u4f53\u306e\u7279\u7570\u6027\u300d\u3068\u300c\u30dd\u30ea\u30af\u30ed\u30fc\u30ca\u30eb\u6297\u4f53\u306e\u611f\u5ea6\u300d\u3092\u4f75\u305b\u6301\u3064\u3001\u3053\u308c\u307e\u3067\u306b\u306f\u306a\u3044\u65b0\u767a\u60f3\u306e\u4e8c\u6b21\u6297\u4f53\u3067\u3059\u3002<\/p>\n<ul style=\"list-style-type: disc\">\n<li>\u30e2\u30ce\u30af\u30ed\u30fc\u30ca\u30eb\u6297\u4f53\u540c\u69d8\u306b\u6297\u4f53\u7523\u751f\u7d30\u80de\u3092\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3057\u3001\u3055\u3089\u306b\u907a\u4f1d\u5b50\u3092\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30fb\u4fdd\u5b58\u3057\u3066\u3044\u308b\u306e\u3067\u3001\u30ed\u30c3\u30c8\u9593\u5dee\u304c\u6975\u3081\u3066\u5c0f\u3055\u3044\u3002<\/li>\n<li>\u7279\u7570\u6027\u3084\u611f\u5ea6\u304c\u9ad8\u3044\u8907\u6570\u306e\u30af\u30ed\u30fc\u30f3\u3092\u9078\u629e\u3057\u3001\u6297\u4f53\u3092\u30d7\u30fc\u30eb\u3057\u3066\u88fd\u54c1\u5316\u3057\u3066\u3044\u308b\u306e\u3067\u30dd\u30ea\u30af\u30ed\u30fc\u30ca\u30eb\u6297\u4f53\u306e\u3088\u3046\u306b\u9ad8\u611f\u5ea6\u3002<\/li>\n<\/ul>\n<p><a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/antibodies\/secondary-antibodies\/superclonal-secondary-antibodies.html?cid=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_ca2n_Social_LAB\" target=\"_blank\" rel=\"noopener\">Superclonal\u4e8c\u6b21\u6297\u4f53\u306e\u8a73\u7d30\u60c5\u5831\u306f\u3053\u3061\u3089\u304b\u3089<\/a><\/p>\n<h2><span style=\"font-size: 12pt\">\u30e9\u30a4\u30d5\u30b5\u30a4\u30a8\u30f3\u30b9\u60c5\u5831\u8a8c\u300cNEXT\u300d<\/span><\/h2>\n<p><a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/communities-social\/NEXT-Forum\/next\/next-backnumber.html?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_ca2n_Social_LAB\" target=\"_blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" class=\"alignleft size-full wp-image-3659\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/foregroundimg.img_.full_.high_-6.png\" alt=\"NEXT2017\u5e747\u6708\u53f7\" width=\"167\" height=\"235\" \/><\/a>\u5f53\u8a18\u4e8b\u306f\u30b5\u30fc\u30e2\u30d5\u30a3\u30c3\u30b7\u30e3\u30fc\u30b5\u30a4\u30a8\u30f3\u30c6\u30a3\u30d5\u30a3\u30c3\u30af\u304c\u767a\u520a\u3059\u308b\u30e9\u30a4\u30d5\u30b5\u30a4\u30a8\u30f3\u30b9\u60c5\u5831\u8a8c\u300cNEXT\u300d2017\u5e747\u6708\u53f7\u304b\u3089\u306e\u629c\u7c8b\u3067\u3059\u3002<br \/>\n<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/communities-social\/NEXT-Forum\/next\/next-backnumber.html?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_ca2n_Social_LAB\" target=\"_blank\" rel=\"noopener\">NEXT\u306e\u30d0\u30c3\u30af\u30ca\u30f3\u30d0\u30fc\u306f\u3053\u3061\u3089<\/a><\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u7528\u306b\u306f\u4f7f\u7528\u3044\u305f\u3060\u3051\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u80da\u767a\u751f\u306b\u91cd\u8981\u306a\u5f79\u5272\u3092\u62c5\u3046\u907a\u4f1d\u5b50\u3092\u907a\u4f1d\u5b50\u6539\u5909\u30de\u30a6\u30b9\u3067\u89e3\u6790 Dinh Thi Huong Tra \u6c0f\uff08 \u7b51\u6ce2\u5927\u5b66\u5927\u5b66\u9662\u535a\u58eb\u8ab2\u7a0b\uff09 \u6c34\u91ce\u8056\u54c9 \u6c0f\uff08 \u7b51\u6ce2\u5927\u5b66\u533b\u5b66\u533b\u7642\u7cfb\u30fb\u751f\u547d\u79d1\u5b66\u52d5\u7269\u8cc7\u6e90\u30bb\u30f3\u30bf\u30fc\u52a9\u6559\uff09 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