{"id":14795,"date":"2023-07-21T09:50:38","date_gmt":"2023-07-21T00:50:38","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/?p=14795"},"modified":"2024-09-18T16:06:40","modified_gmt":"2024-09-18T07:06:40","slug":"ce-workshop-gsd-ts-2","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/ce-workshop-gsd-ts-2\/","title":{"rendered":"\u3010\u3084\u3063\u3066\u307f\u305f\u3011\u30b5\u30a4\u30af\u30eb\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u5f8c\u3001\u7cbe\u88fd\u305b\u305a\u306b\u6cf3\u52d5\u3057\u3066\u307f\u305f"},"content":{"rendered":"<p>\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u306b\u306f\u3001\u86cd\u5149\u6a19\u8b58\u30b8\u30c7\u30aa\u30ad\u30b7\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\uff08\u30c0\u30a4\u30bf\u30fc\u30df\u30cd\u30fc\u30bf\u30fc\uff09\u3092\u7528\u3044\u308b\u3053\u3068\u3067\u3001\u4f38\u9577\u7523\u7269\u306b\u86cd\u5149\u304c\u6a19\u8b58\u3055\u308c\u307e\u3059\u3002\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u5f8c\u306e\u53cd\u5fdc\u6db2\u4e2d\u306b\u306f\u3001\u4f7f\u308f\u308c\u306a\u304b\u3063\u305f\u30c0\u30a4\u30bf\u30fc\u30df\u30cd\u30fc\u30bf\u30fc\u304c\u5b58\u5728\u3057\u307e\u3059\u3002\u6b8b\u5b58\u306e\u30c0\u30a4\u30bf\u30fc\u30df\u30cd\u30fc\u30bf\u30fc\u304c\u4f38\u9577\u7523\u7269\u3068\u3068\u3082\u306b\u30ad\u30e3\u30d4\u30e9\u30ea\u30fc\u306b\u53d6\u308a\u8fbc\u307e\u308c\u308b\u3068\u3001\u5927\u304d\u306a\u30d4\u30fc\u30af\uff08Dye Blob\u30d4\u30fc\u30af\uff09\u3068\u3057\u3066\u691c\u51fa\u3055\u308c\u3001\u305d\u306e\u90e8\u5206\u306e\u30d9\u30fc\u30b9\u30b3\u30fc\u30eb\u306e\u7cbe\u5ea6\u3092\u4f4e\u4e0b\u3055\u305b\u307e\u3059\u3002\u3053\u306e\u305f\u3081\u3001\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u5f8c\u306f\u3001\u672a\u53cd\u5fdc\u306e\u30c0\u30a4\u30bf\u30fc\u30df\u30cd\u30fc\u30bf\u30fc\u3092\u53d6\u308a\u9664\u304f\u7cbe\u88fd\u304c\u5fc5\u8981\u3068\u306a\u308a\u307e\u3059\u3002<br \/>\n\u4e00\u65b9\u3067\u3001\u300c\u30b5\u30f3\u30d7\u30eb\u6570\u304c\u591a\u3044\u5834\u5408\u3001\u7cbe\u88fd\u5de5\u7a0b\u306f\u624b\u9593\u3068\u611f\u3058\u308b\u300d\u3001\u300c\u76ee\u7684\u9818\u57df\u3068Dye Blob\u30d4\u30fc\u30af\u304c\u91cd\u306a\u3089\u306a\u3051\u308c\u3070\u3001\u7cbe\u88fd\u306f\u4e0d\u8981\u3067\u3059\u304b\uff1f\u300d\u3068\u306e\u304a\u58f0\u3092\u3044\u305f\u3060\u3044\u305f\u3053\u3068\u304c\u3042\u308a\u307e\u3057\u305f\u3002\u305d\u3053\u3067\u3001\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u5f8c\u3001\u7cbe\u88fd\u305b\u305a\u306b\u305d\u306e\u307e\u307e\u30b8\u30a7\u30cd\u30c6\u30a3\u30c3\u30af\u30a2\u30ca\u30e9\u30a4\u30b6\u3067\u30e9\u30f3\u3059\u308b\u3068\u3001\u3069\u306e\u3088\u3046\u306a\u7d50\u679c\u306b\u306a\u308b\u304b\u3001\u691c\u8a3c\u3057\u3066\u307f\u307e\u3057\u305f\u3002<\/p>\n<h2>\u5b9f\u9a13<\/h2>\n<p><b>\u4f7f\u7528\u8a66\u85ac<\/b><\/p>\n<ul>\n<li>pGEM -3Zf(+) double stranded DNA Control Template (\u203b)<\/li>\n<li>-21 M13 Control Primer (\u203b)<\/li>\n<li>Applied Biosystems\u2122 <a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/4337455?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_og_s00_ce_workshop_gsd_ts_2_Social_LAB\" target=\"_blank\" rel=\"noopener\">BigDye\u2122 Terminator v3.1 Cycle Sequencing Kit<\/a><br \/>\n\u203bBigDye\u2122 Terminator v3.1 Cycle Sequencing Kit\u306b\u542b\u307e\u308c\u307e\u3059\u3002<\/li>\n<\/ul>\n<p><b>\u4f7f\u7528\u6a5f\u5668<\/b><\/p>\n<ul>\n<li>\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\uff1a\u3000Applied Biosystems\u2122 <a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/pcr\/thermal-cyclers-realtime-instruments\/thermal-cyclers\/proflex-pcr-system.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_og_s00_ce_workshop_gsd_ts_2_Social_LAB\" target=\"_blank\" rel=\"noopener\">ProFlex\u2122 PCR System<\/a><\/li>\n<li>\u30b7\u30fc\u30b1\u30f3\u30b5\u30fc\uff1a\u3000Applied Biosystems\u2122 <a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/sequencing\/sanger-sequencing\/genetic-analyzers\/models\/seqstudio.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_og_s00_ce_workshop_gsd_ts_2_Social_LAB\" target=\"_blank\" rel=\"noopener\">SeqStudio\u2122 Genetic Analyzer<\/a><\/li>\n<\/ul>\n<p><b>\u5b9f\u9a13<\/b><\/p>\n<p>\u88681\u30012\u306e\u6761\u4ef6\u3067\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u5f8c\u3001\u4f38\u9577\u7523\u7269\u3092\u7cbe\u88fd\u305b\u305a\u306b\u96fb\u6c17\u6cf3\u52d5\u3092\u884c\u3044\u307e\u3057\u305f\u3002\u96fb\u6c17\u6cf3\u52d5\u306f\u3001SeqStudio Genetic Analyzer\u3092\u4f7f\u7528\u3057\u3001MediumSeq\u306e\u6761\u4ef6\u3067\u5b9f\u65bd\u3057\u307e\u3057\u305f\u3002<\/p>\n<table style=\"border-color: silver\" border=\"1\">\n<caption>\u88681 \u53cd\u5fdc\u6db2\u7d44\u6210<\/caption>\n<tbody>\n<tr bgcolor=\"gainsboro\">\n<td style=\"text-align: center\" width=\"346\"><b>\u7d44\u6210<\/b><\/td>\n<td style=\"text-align: center\" width=\"76\"><b>\u91cf\/\u53cd\u5fdc<\/b><\/td>\n<\/tr>\n<tr>\n<td width=\"346\">\u00a0BigDye\u2122 Terminator v3.1 Ready Reaction Mix<\/td>\n<td style=\"text-align: center\" width=\"76\">4 \u00b5L<\/td>\n<\/tr>\n<tr>\n<td width=\"346\">\u00a0x5 Sequencing Buffer<\/td>\n<td style=\"text-align: center\" width=\"76\">2 \u00b5L<\/td>\n<\/tr>\n<tr>\n<td width=\"346\">\u00a0pGEM -3Zf(+) DNA Control Template\uff08200 ng\/\u00b5L\uff09<\/td>\n<td style=\"text-align: center\" width=\"76\">1 \u00b5L<\/td>\n<\/tr>\n<tr>\n<td width=\"346\">\u00a0-21 M13 Control Primer\uff080.8 pmol\/\u00b5L\uff09<\/td>\n<td style=\"text-align: center\" width=\"76\">4 \u00b5L<\/td>\n<\/tr>\n<tr>\n<td width=\"346\">\u00a0\u8131\u30a4\u30aa\u30f3\u6c34<\/td>\n<td style=\"text-align: center\" width=\"76\">9 \u00b5L<\/td>\n<\/tr>\n<tr>\n<td width=\"346\">\u00a0\u5168\u91cf<\/td>\n<td style=\"text-align: center\" width=\"76\">20 \u00b5L<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<table style=\"border-color: silver\" border=\"1\">\n<caption>\u88682\u3000\u30b5\u30a4\u30af\u30eb\u30b7\u30fc\u30b1\u30f3\u30b9\u30d7\u30ed\u30b0\u30e9\u30e0<\/caption>\n<tbody>\n<tr bgcolor=\"darkgray\">\n<td rowspan=\"2\" width=\"49\"><\/td>\n<td style=\"text-align: center\" colspan=\"5\" width=\"373\"><span style=\"color: #ffffff\"><b>\u30b9\u30c6\u30c3\u30d7<\/b><\/span><\/td>\n<\/tr>\n<tr bgcolor=\"gainsboro\">\n<td style=\"text-align: center\" width=\"75\"><b>\u30db\u30fc\u30eb\u30c9<b><\/b><\/b><\/td>\n<td style=\"text-align: center\" colspan=\"3\" width=\"224\"><b>25 \u30b5\u30a4\u30af\u30eb<\/b><\/td>\n<td style=\"text-align: center\" width=\"75\"><b>\u30db\u30fc\u30eb\u30c9<\/b><\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"49\">\u6e29\u5ea6<\/td>\n<td style=\"text-align: center\" width=\"75\">96\u2103<\/td>\n<td style=\"text-align: center\" width=\"75\">96\u2103<\/td>\n<td style=\"text-align: center\" width=\"75\">50\u2103<\/td>\n<td style=\"text-align: center\" width=\"75\">60\u2103<\/td>\n<td style=\"text-align: center\" width=\"75\">4\u2103<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"49\">\u6642\u9593<\/td>\n<td style=\"text-align: center\" width=\"75\">60\u79d2<\/td>\n<td style=\"text-align: center\" width=\"75\">10\u79d2<\/td>\n<td style=\"text-align: center\" width=\"75\">5\u79d2<\/td>\n<td style=\"text-align: center\" width=\"75\">75\u79d2<\/td>\n<td style=\"text-align: center\" width=\"75\">\u221e<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h2>\u7d50\u679c<\/h2>\n<p>\u5f97\u3089\u308c\u305fRaw\u30c7\u30fc\u30bf\u3092\u56f31\u306b\u793a\u3057\u307e\u3059\u3002\u30b7\u30fc\u30b1\u30f3\u30b9\u7523\u7269\u306e\u30d4\u30fc\u30af\u3088\u308a\u3082\u5927\u304d\u306a\u30b7\u30b0\u30ca\u30eb\u5f37\u5ea6\u3067\u30014\u3064\u306e\u30d4\u30fc\u30af\u304c\u307f\u3089\u308c\uff08\u56f31(a) \u77e2\u5370\uff09\u3001\u30d4\u30fc\u30af\u4f4d\u7f6e\u306f\u3001\uff5e65\u300165\uff5e90\u300190\uff5e120\u3001280\uff5e310 base\u3067\u3057\u305f\u3002\u3053\u308c\u30894\u3064\u306e\u30d4\u30fc\u30af\u3088\u308a\u4f4e\u3044\u30b7\u30b0\u30ca\u30eb\u5f37\u5ea6\u306e\u3001\u30d6\u30ed\u30fc\u30c9\u306a\u30d4\u30fc\u30af\u3082\u8907\u6570\u89b3\u5bdf\u3055\u308c\u307e\u3057\u305f\uff08\u56f31(b) \u70b9\u7dda\u67a0\u5185\uff09\u3002\u3053\u308c\u3089\u306f\u3044\u305a\u308c\u3082\u3001\u672a\u53cd\u5fdc\u306e\u30c0\u30a4\u30bf\u30fc\u30df\u30cd\u30fc\u30bf\u30fc\u306b\u7531\u6765\u3059\u308b\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002<\/p>\n<div id=\"attachment_14820\" style=\"width: 1264px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-14820\" class=\"size-full wp-image-14820\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/07\/ce-workshop-gsd-ts-2-01.png\" alt=\"\u672a\u7cbe\u88fd\u30b7\u30fc\u30b1\u30f3\u30b9\u7523\u7269\u306eRaw\u30c7\u30fc\u30bf\" width=\"1254\" height=\"511\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/07\/ce-workshop-gsd-ts-2-01.png 1254w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/07\/ce-workshop-gsd-ts-2-01-300x122.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/07\/ce-workshop-gsd-ts-2-01-1024x417.png 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/07\/ce-workshop-gsd-ts-2-01-768x313.png 768w\" sizes=\"auto, (max-width: 1254px) 100vw, 1254px\" \/><p id=\"caption-attachment-14820\" class=\"wp-caption-text\">\u56f31 \u672a\u7cbe\u88fd\u30b7\u30fc\u30b1\u30f3\u30b9\u7523\u7269\u306eRaw\u30c7\u30fc\u30bf<br \/>(a) \u7e26\u8ef8\u6700\u5927\u5024\u300032000 RFU\u3001(b) \u7e26\u8ef8\u6700\u5927\u5024\u30001200 RFU<\/p><\/div>\n<p>Quality Value\uff08QV\uff09\u306f\u3001\u5869\u57fa\u5f53\u305f\u308a\u306e\u30d9\u30fc\u30b9\u30b3\u30fc\u30eb\u7cbe\u5ea6\u306e\u6307\u6a19\u3068\u306a\u308b\u5024\u3067\u3001\u56f32\u3067\u306f\u3001\u30a8\u30ec\u30af\u30c8\u30ed\u30d5\u30a7\u30ed\u30b0\u30e9\u30e0\u306e\u4e0a\u306bQV\u30d0\u30fc\u3068\u3057\u3066\u8868\u793a\u3055\u308c\u3066\u3044\u307e\u3059\u3002QV\u30d0\u30fc\u304c\u9ad8\u3044\u307b\u3069QV\u306e\u5024\u304c\u9ad8\u3044\u3053\u3068\u3092\u793a\u3057\u3066\u304a\u308a\u3001\u56f32\u3067\u306f\u3001\u30d0\u30fc\u304c\u9752\u8272\u306e\u5869\u57fa\u306f\u3001\u826f\u597d\u306a\u7cbe\u5ea6\u3067\u30d9\u30fc\u30b9\u30b3\u30fc\u30eb\u3067\u304d\u3066\u3044\u308b\u3053\u3068\u3092\u793a\u3057\u307e\u3059\u3002\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u5f8c\u3001\u7cbe\u88fd\u305b\u305a\u306b\u6cf3\u52d5\u3092\u884c\u3046\u3068\u3001\u524d\u8ff0\u306e\u5927\u304d\u306a\u30d4\u30fc\u30af\u306e\u6240\u306b\u52a0\u3048\u3001\u8907\u6570\u306e\u30d6\u30ed\u30fc\u30c9\u30d4\u30fc\u30af\u306e\u7b87\u6240\u3067\u3001\u5e83\u7bc4\u56f2\u306b\u308f\u305f\u308a\u30d9\u30fc\u30b9\u30b3\u30fc\u30eb\u306e\u7cbe\u5ea6\u304c\u60aa\u5316\u3057\u307e\u3057\u305f\uff08\u56f32 (a)\uff09\u3002\u6bd4\u8f03\u30c7\u30fc\u30bf\u3068\u3057\u3066\u3001\u88681\u30012\u306e\u6761\u4ef6\u3067\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u5f8c\u3001Applied Biosystems\u2122 <a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/4376486?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_og_s00_ce_workshop_gsd_ts_2_Social_LAB\" target=\"_blank\" rel=\"noopener\">BigDye XTerminator\u2122 Purification Kit<\/a>\u306b\u3088\u308a\u7cbe\u88fd\u3057\u305f\u5834\u5408\u306e\u30c7\u30fc\u30bf\u3092\u56f32 (b)\u306b\u793a\u3057\u307e\u3059\u3002\u30a8\u30ec\u30af\u30c8\u30ed\u30d5\u30a7\u30ed\u30b0\u30e9\u30e0\u306e\u4e0a\u306b\u306f\u9752\u8272\u306eQV\u30d0\u30fc\u304c\u9023\u7d9a\u3057\u3066\u304a\u308a\u3001\u826f\u597d\u306a\u7cbe\u5ea6\u3067\u30d9\u30fc\u30b9\u30b3\u30fc\u30eb\u3055\u308c\u305f\u3053\u3068\u304c\u78ba\u8a8d\u3067\u304d\u307e\u3057\u305f\u3002<\/p>\n<div id=\"attachment_14821\" style=\"width: 1266px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-14821\" class=\"size-full wp-image-14821\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/07\/ce-workshop-gsd-ts-2-02.png\" alt=\"\u30b7\u30fc\u30b1\u30f3\u30b9\u7523\u7269\u306e\u30a8\u30ec\u30af\u30c8\u30ed\u30d5\u30a7\u30ed\u30b0\u30e9\u30e0\uff080-390 bp\uff09\" width=\"1256\" height=\"597\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/07\/ce-workshop-gsd-ts-2-02.png 1256w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/07\/ce-workshop-gsd-ts-2-02-300x143.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/07\/ce-workshop-gsd-ts-2-02-1024x487.png 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/07\/ce-workshop-gsd-ts-2-02-768x365.png 768w\" sizes=\"auto, (max-width: 1256px) 100vw, 1256px\" \/><p id=\"caption-attachment-14821\" class=\"wp-caption-text\">\u56f32\u3000\u30b7\u30fc\u30b1\u30f3\u30b9\u7523\u7269\u306e\u30a8\u30ec\u30af\u30c8\u30ed\u30d5\u30a7\u30ed\u30b0\u30e9\u30e0\uff080-390 bp\uff09<br \/>(a) \u672a\u7cbe\u88fd\u3001(b) BigDye XTerminator\u7cbe\u88fd<\/p><\/div>\n<h2>\u307e\u3068\u3081<\/h2>\n<p>\u4eca\u56de\u306f\u3001SeqStudio Genetic 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href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/technical-resources\/seminars\/dna-sequencing2.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_og_s00_ce_workshop_gsd_ts_2_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u89e3\u6790\u57fa\u790e\u30bb\u30df\u30ca\u30fc\uff082\uff09\u30c7\u30fc\u30bf\u78ba\u8a8d\u3068\u30c8\u30e9\u30d6\u30eb\u30b7\u30e5\u30fc\u30c8<\/a><br \/>\n<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/technical-resources\/trainings\/ab-dna-sequencing3130-3130xL.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_og_s00_ce_workshop_gsd_ts_2_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u5b8c\u5168\u30de\u30b9\u30bf\u30fc\uff01\u30cf\u30f3\u30ba\u30aa\u30f3\u30c8\u30ec\u30fc\u30cb\u30f3\u30b0\u30b3\u30fc\u30b9\u3000\u30ad\u30e3\u30d4\u30e9\u30ea\u30fc\u30b7\u30fc\u30b1\u30f3\u30b5(1) 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