{"id":14862,"date":"2023-07-27T16:50:06","date_gmt":"2023-07-27T07:50:06","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/?p=14862"},"modified":"2025-03-06T17:31:43","modified_gmt":"2025-03-06T08:31:43","slug":"rna_workshop_bid_ts_5","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/rna_workshop_bid_ts_5\/","title":{"rendered":"\u3010\u3084\u3063\u3066\u307f\u305f\u3011RNA\u62bd\u51fa\u306b\u4f7f\u3046Wash Buffer\u306b\u30a8\u30bf\u30ce\u30fc\u30eb\u3092\u52a0\u3048\u5fd8\u308c\u305f\u3089\uff1f"},"content":{"rendered":"

RNA\u62bd\u51fa\u306b\u306f\u5bfe\u8c61\u30b5\u30f3\u30d7\u30eb\u306b\u5408\u308f\u305b\u3066\u3055\u307e\u3056\u307e\u306a\u65b9\u6cd5\u304c\u3042\u308a\u307e\u3059\u3002\u4e2d\u3067\u3082\u30b7\u30ea\u30ab\u30e1\u30f3\u30d6\u30ec\u30f3\u30bf\u30a4\u30d7\u306e\u30b9\u30d4\u30f3\u30ab\u30e9\u30e0\u3092\u4f7f\u7528\u3057\u305f\u7cbe\u88fd\u65b9\u6cd5\u306f\u8ab0\u3067\u3082\u7c21\u5358\u306b\u77ed\u6642\u9593\u3067RNA\u3092\u56de\u53ce\u3067\u304d\u308b\u65b9\u6cd5\u306e1\u3064\u3067\u3059\u3002<\/p>\n

\u306f\u3058\u3081\u306b<\/h2>\n

\u30b9\u30d4\u30f3\u30ab\u30e9\u30e0\u3067RNA\u62bd\u51fa\u3059\u308b\u30ad\u30c3\u30c8\u306f\u5f53\u793e\u3060\u3051\u3067\u306a\u304f\u3001\u3055\u307e\u3056\u307e\u306a\u30e1\u30fc\u30ab\u30fc\u304b\u3089\u8ca9\u58f2\u3055\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u57fa\u672c\u7684\u306a\u539f\u7406\u306f\u307b\u3068\u3093\u3069\u4e00\u7dd2\u306e\u306f\u305a\u3067\u3059\u3002\u4eca\u56de\u306f\u3001\u5f53\u793e\u306eInvitrogen\u2122 PureLink\u2122 RNA Mini \u30ad\u30c3\u30c8<\/a>\uff08\u88fd\u54c1\u756a\u53f712183020\uff09\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002
\n\u672c\u30ad\u30c3\u30c8\u304c\u304a\u624b\u5143\u306b\u5c4a\u3044\u305f\u5f8c\u3001\u6700\u521d\u306b\u5b9f\u65bd\u3057\u3066\u3044\u305f\u3060\u304f\u64cd\u4f5c\u306fWash Buffer II\u306b\u30a8\u30bf\u30ce\u30fc\u30eb\u3092\u898f\u5b9a\u91cf\u52a0\u3048\u3066\u3044\u305f\u3060\u304f\u3053\u3068\u3067\u3059\u30024 mL\u306eWash Buffer II\u306b\u5bfe\u3057\u3066\u300116 mL\u306e96\uff5e100%\u30a8\u30bf\u30ce\u30fc\u30eb\u3092\u52a0\u3048\u3066\u6df7\u5408\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\uff08\u7d42\u6fc3\u5ea680\u5bb9\u91cf%\uff09\u3002\u8a66\u85ac\u306e\u30dc\u30c8\u30eb\u306b\u3082\u30a8\u30bf\u30ce\u30fc\u30eb\u3092\u6dfb\u52a0\u3057\u305f\u3053\u3068\u3092\u8a18\u9332\u3059\u308b\u30c1\u30a7\u30c3\u30af\u30dc\u30c3\u30af\u30b9\u306f\u3042\u308b\u306e\u3067\u3059\u304c\u3001\u4f7f\u7528\u9014\u4e2d\u3067\u6db2\u91cf\u304c\u6e1b\u3063\u3066\u304f\u308b\u3068\u672c\u5f53\u306b\u30a8\u30bf\u30ce\u30fc\u30eb\u3092\u5165\u308c\u305f\u306e\u304b\u4e0d\u5b89\u306b\u306a\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u3067\u306f\u3001\u3046\u3063\u304b\u308a\u30a8\u30bf\u30ce\u30fc\u30eb\u3092\u6dfb\u52a0\u3057\u5fd8\u308c\u305f\u307e\u307e\u5b9f\u9a13\u3059\u308b\u3068\u3069\u3046\u306a\u308b\u306e\u3067\u3057\u3087\u3046\u304b\uff1f<\/p>\n

\u7d50\u679c\u3068\u8003\u5bdf<\/h2>\n

\u307e\u305a\u306fRNA\u62bd\u51fa\u306e\u7d50\u679c\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002
\n\"\"
\n\"\"<\/p>\n

\u30a8\u30bf\u30ce\u30fc\u30eb\u3092\u6dfb\u52a0\u306a\u3057\u306eWash Buffer II\u3092\u4f7f\u7528\u3059\u308b\u3068\u3001RNA\u3092\u307b\u3068\u3093\u3069\u56de\u53ce\u3067\u304d\u307e\u305b\u3093\u3067\u3057\u305f\u3002\u3064\u307e\u308a\u3001Lysis Buffer\u3067\u6eb6\u89e3\u3001\u30b7\u30ea\u30ab\u30e1\u30f3\u30d6\u30ec\u30f3\u3078\u306e\u30a2\u30d7\u30e9\u30a4\u3001Wash Buffer I\u30671\u56de\u6d17\u6d44\u3001Wash Buffer II\u30672\u56de\u6d17\u6d44\u3001RNase-Free Water\u3067\u6eb6\u51fa\u3001\u3068\u3044\u3046\u624b\u9806\u306f\u5171\u901a\u3060\u3063\u305f\u306e\u306b\u3001Wash Buffer II\u3078\u306e\u30a8\u30bf\u30ce\u30fc\u30eb\u6dfb\u52a0\u306e\u6709\u7121\u306e\u307f\u3067RNA\u304c\u56de\u53ce\u3067\u304d\u307e\u305b\u3093\u3067\u3057\u305f\u3002<\/p>\n

\u30a8\u30bf\u30ce\u30fc\u30eb\u3092\u6dfb\u52a0\u3057\u5fd8\u308c\u308b\u3068\u3001\u306a\u305cRNA\u62bd\u51fa\u306b\u5931\u6557\u3057\u3066\u3057\u307e\u3046\u306e\u3067\u3057\u3087\u3046\u304b\u3002\u307e\u305a\u306f\u3001\u30b7\u30ea\u30ab\u30e1\u30f3\u30d6\u30ec\u30f3\u3092\u7528\u3044\u305fRNA\u62bd\u51fa\u306e\u539f\u7406\u304b\u3089\u8003\u3048\u3066\u307f\u307e\u3057\u3087\u3046\u3002\u901a\u5e38\u306e\u6c34\u3084Buffer\u306a\u3069\u306b\u6eb6\u3051\u3066\u3044\u308bRNA\u306f\u30b7\u30ea\u30ab\u30e1\u30f3\u30d6\u30ec\u30f3\u306b\u7d50\u5408\u3059\u308b\u3053\u3068\u306f\u3067\u304d\u307e\u305b\u3093\u3002\u3057\u304b\u3057\u3001\u9ad8\u6fc3\u5ea6\u306e\u30ab\u30aa\u30c8\u30ed\u30d4\u30c3\u30af\u5869\uff08\u30b0\u30a2\u30cb\u30b8\u30f3\u30a4\u30bd\u30c1\u30aa\u30b7\u30a2\u30cd\u30fc\u30c8\uff09\u3084\u30a8\u30bf\u30ce\u30fc\u30eb\u306e\u5b58\u5728\u4e0b\u3067\u306f\u3001RNA\u304b\u3089\u6c34\u5206\u5b50\u304c\u596a\u308f\u308c\u308b\u3053\u3068\u3067\u758e\u6c34\u6027\u304c\u9ad8\u307e\u308aRNA\u304c\u30b7\u30ea\u30ab\u30e1\u30f3\u30d6\u30ec\u30f3\u306b\u7d50\u5408\u3067\u304d\u308b\u3088\u3046\u306b\u306a\u308a\u307e\u3059\u3002
\n\u6b21\u306b\u3001\u305d\u308c\u305e\u308c\u306eBuffer\u306e\u9055\u3044\u3092\u8003\u3048\u3066\u307f\u307e\u3057\u3087\u3046\u3002Lysis Buffer\u3084Wash Buffer I\u306f\u30ab\u30aa\u30c8\u30ed\u30d4\u30c3\u30af\u5869\u304c\u542b\u307e\u308c\u3066\u3044\u307e\u3059\u3002\u307e\u305f\u3001Wash Buffer II\u306b\u306f\u30a8\u30bf\u30ce\u30fc\u30eb\u3092\u52a0\u3048\u307e\u3059\u3002\u3053\u306e\u305f\u3081\u3001\u6eb6\u89e3\u5f8c\u306e\u30ab\u30e9\u30e0\u3078\u306e\u30a2\u30d7\u30e9\u30a4\u6642\u3084Wash\u6642\u306b\u306fRNA\u306e\u758e\u6c34\u6027\u304c\u7dad\u6301\u3055\u308c\u3066\u3044\u308b\u305f\u3081\u306b\u3001RNA\u304c\u30b7\u30ea\u30ab\u30e1\u30f3\u30d6\u30ec\u30f3\u306b\u7d50\u5408\u3067\u304d\u3066\u3044\u308b\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002\u3053\u306e\u3088\u3046\u306a\u80cc\u666f\u304b\u3089\u3001Wash Buffer II\u306b\u30a8\u30bf\u30ce\u30fc\u30eb\u3092\u3046\u3063\u304b\u308a\u52a0\u3048\u5fd8\u308c\u308b\u3068RNA\u306e\u758e\u6c34\u6027\u304c\u5931\u308f\u308c\u3066\u3057\u307e\u3044\u3001\u30b7\u30ea\u30ab\u30e1\u30f3\u30d6\u30ec\u30f3\u306b\u7d50\u5408\u3067\u304d\u305a\u3001\u30d5\u30ed\u30fc\u30b9\u30eb\u30fc\u306b\u6d41\u308c\u3066\u3057\u307e\u3063\u305f\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002\u305d\u3093\u306a\u3053\u3068\u306a\u3089\u3001\u5546\u54c1\u306b\u306f\u3058\u3081\u304b\u3089\u30a8\u30bf\u30ce\u30fc\u30eb\u3092\u6dfb\u52a0\u3057\u3066\u304a\u3044\u3066\u304f\u308c\u308c\u3070\u826f\u3044\u306e\u306b\u3001\u3068\u3044\u3046\u304a\u58f0\u304c\u805e\u3053\u3048\u3066\u304d\u305d\u3046\u3067\u3059\u3002\u6700\u521d\u306b\u3054\u8aac\u660e\u3057\u305f\u3088\u3046\u306b\u3001Wash Buffer II\u306f\u30a8\u30bf\u30ce\u30fc\u30eb\u306e\u7d42\u6fc3\u5ea680\u5bb9\u91cf%\u3067\u4f7f\u7528\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u3057\u304b\u3057\u3001\u30a2\u30eb\u30b3\u30fc\u30eb\u6fc3\u5ea6\u304c\u7d0467\u5bb9\u91cf%\u4ee5\u4e0a\u306b\u306a\u308b\u3068\u3001\u6d88\u9632\u6cd5\u3067\u5b9a\u3081\u308b\u5371\u967a\u7269\u3068\u306a\u3063\u3066\u3057\u307e\u3044\u8f38\u9001\u6642\u306b\u3055\u307e\u3056\u307e\u306a\u6ce8\u610f\u304c\u5fc5\u8981\u306b\u306a\u308a\u307e\u3059\u3002\u3053\u306e\u305f\u3081\u3001\u304a\u624b\u6570\u3092\u304a\u639b\u3051\u3057\u307e\u3059\u304c\u3001\u30ad\u30c3\u30c8\u3092\u304a\u5c4a\u3051\u3057\u305f\u5f8c\u3001\u304a\u5ba2\u3055\u307e\u306b\u30a8\u30bf\u30ce\u30fc\u30eb\u6dfb\u52a0\u3092\u304a\u9858\u3044\u3057\u3066\u3044\u308b\u6b21\u7b2c\u3067\u3059\u3002<\/p>\n

\u307e\u3068\u3081<\/h2>\n

\u4eca\u56de\u306f\u3001PureLink RNA Mini \u30ad\u30c3\u30c8<\/a>\u306eWash Buffer II\u306b\u30a8\u30bf\u30ce\u30fc\u30eb\u3092\u3046\u3063\u304b\u308a\u6dfb\u52a0\u3057\u5fd8\u308c\u3066\u3057\u307e\u3063\u305f\u5834\u5408\u306e\u7d50\u679c\u3092\u304a\u793a\u3057\u3057\u307e\u3057\u305f\u3002\u30ad\u30c3\u30c8\u3092\u958b\u68b1\u3057\u305f\u65b9\u306f\u3001Wash Buffer II\u306e\u30dc\u30c8\u30eb\u306b\u898f\u5b9a\u91cf\u306e\u30a8\u30bf\u30ce\u30fc\u30eb\u3092\u6dfb\u52a0\u3057\u3001\u30dc\u30c8\u30eb\u306b\u3042\u308b\u30c1\u30a7\u30c3\u30af\u30dc\u30c3\u30af\u30b9\u3078\u306e\u8a18\u9332\u3082\u5fd8\u308c\u306a\u3044\u3088\u3046\u306b\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u307e\u305f\u3001\u4f7f\u7528\u9014\u4e2d\u306e\u30ad\u30c3\u30c8\u3092\u4f7f\u7528\u3055\u308c\u308b\u5834\u5408\u306f\u3001\u30a8\u30bf\u30ce\u30fc\u30eb\u6dfb\u52a0\u306e\u8a18\u9332\u304c\u3042\u308b\u3053\u3068\u3092\u3054\u78ba\u8a8d\u304f\u3060\u3055\u3044\u3002\u4eca\u56de\u306e\u3088\u3046\u306bRNA\u62bd\u51fa\u306b\u5931\u6557\u3057\u306a\u3044\u3088\u3046\u3001\u53d6\u6271\u8aac\u660e\u66f8\u306b\u5f93\u3063\u3066\u30a8\u30bf\u30ce\u30fc\u30eb\u3092\u6dfb\u52a0\u3057\u3066\u304b\u3089\u5b9f\u9a13\u3092\u59cb\u3081\u3066\u3044\u305f\u3060\u3051\u308c\u3070\u3068\u601d\u3044\u307e\u3059\u3002
\n\"\"<\/p>\n

\u5f53\u793e\u3067\u306fRNA\u62bd\u51fa\u3084\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3001\u4ed6\u306b\u3082\u7d30\u80de\u57f9\u990a\u3001\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306a\u3069\u3001\u5b9f\u969b\u306b\u5b9f\u9a13\uff08\u5b9f\u7fd2\uff09\u3092\u884c\u3044\u3064\u3064\u5b66\u3079\u308b\u5404\u7a2e\u30cf\u30f3\u30ba\u30aa\u30f3\u30c8\u30ec\u30fc\u30cb\u30f3\u30b0<\/a>\u3092\u958b\u50ac\u3057\u3066\u3044\u307e\u3059\u3002\u305d\u306e\u4e2d\u3067\u4eca\u56de\u306e\u3088\u3046\u306a\u5b9f\u9a13\u7d50\u679c\u3082\u3054\u7d39\u4ecb\u3057\u3066\u3044\u307e\u3059\u306e\u3067\u3001\u3053\u308c\u304b\u3089\u65b0\u3057\u3044\u5b9f\u9a13\u3092\u59cb\u3081\u3089\u308c\u308b\u65b9\u3001\u3088\u308a\u7406\u89e3\u3092\u6df1\u3081\u305f\u3044\u65b9\u306f\u305c\u3072\u3054\u53c2\u52a0\u304f\u3060\u3055\u3044\uff01<\/p>\n

DNA\/RNA\u30ec\u30d5\u30a1\u30ec\u30f3\u30b9\u30ac\u30a4\u30c9\u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u306f\u3053\u3061\u3089<\/a><\/p>\n

DNA\/RNA\u30ec\u30d5\u30a1\u30ec\u30f3\u30b9\u30ac\u30a4\u30c9<\/h3>\n

\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/p>\n

\u30cf\u30f3\u30ba\u30aa\u30f3\u30c8\u30ec\u30fc\u30cb\u30f3\u30b0\u306e\u4e00\u89a7<\/h3>\n

\u8a73\u7d30\u306f\u3053\u3061\u3089<\/a><\/p>\n

\u4eca\u56de\u306e\u8a18\u4e8b\u306b\u95a2\u9023\u3059\u308b\u8a18\u4e8b\uff1a
\nRNA \u62bd\u51fa<\/a>
\nPureLink \u30ad\u30c3\u30c8<\/a>
\n\u6709\u6a5f\u6eb6\u5a92\uff1f\u305d\u308c\u3068\u3082\u30b7\u30ea\u30ab\u30d9\u30fc\u30b9\uff1f \u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306b\u304a\u3051\u308b\u6838\u9178\u306e\u7cbe\u88fd\u304a\u3088\u3073\u5b9a\u91cf\u65b9\u6cd5<\/a>
\n\u3010\u3084\u3063\u3066\u307f\u305f\u3011RNA\u62bd\u51fa\u306b\u30af\u30ed\u30ed\u30db\u30eb\u30e0\u3092\u4f7f\u308f\u305a\u306b\u30d6\u30ed\u30e2\u30af\u30ed\u30ed\u30d7\u30ed\u30d1\u30f3\u3067\u4ee3\u66ff\u3057\u3066\u307f\u305f<\/a>
\n\u3010\u3084\u3063\u3066\u307f\u305f\u3011RNA\u3092\u5e38\u6e29\u3067\u653e\u7f6e\u3057\u3066\u307f\u305f<\/a><\/p>\n

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