{"id":14874,"date":"2023-08-03T11:34:32","date_gmt":"2023-08-03T02:34:32","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/?p=14874"},"modified":"2025-08-12T17:21:52","modified_gmt":"2025-08-12T08:21:52","slug":"western-blot-protein-ts-5","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/western-blot-protein-ts-5\/","title":{"rendered":"\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u8a66\u85ac\u306e\u30dd\u30a4\u30f3\u30c8\uff1a\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u88fd\u54c1\u9078\u3073\u306b\u3082\u3046\u56f0\u3089\u306a\u3044\uff01\u7b2c5\u56de"},"content":{"rendered":"<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-13122\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/12\/western.jpg\" alt=\"westernblotting\" width=\"761\" height=\"476\" 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loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-14881 size-full\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/08\/western-blot-protein-ts-5-01.jpg\" alt=\"\" width=\"1170\" height=\"271\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/08\/western-blot-protein-ts-5-01.jpg 1170w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/08\/western-blot-protein-ts-5-01-300x69.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/08\/western-blot-protein-ts-5-01-1024x237.jpg 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/08\/western-blot-protein-ts-5-01-768x178.jpg 768w\" sizes=\"auto, (max-width: 1170px) 100vw, 1170px\" 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Buffer\u3001\u53f3\uff1a\u5e02\u8ca9\u306e\u30b9\u30ad\u30e0\u30df\u30eb\u30af\uff09<\/p><\/div>\n<p>\u3053\u306e\u3088\u3046\u306b\u3001\u9069\u5207\u306a\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u8a66\u85ac\u3092\u9078\u629e\u3059\u308b\u3053\u3068\u306f\u3001\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9\u3084\u975e\u7279\u7570\u53cd\u5fdc\u3092\u6291\u3048\u308b\u3060\u3051\u3067\u306a\u304f\u3001SN\u6bd4\u3092\u4e0a\u3052\u3001\u3088\u308a\u5fae\u91cf\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u691c\u51fa\u3067\u304d\u308b\u3053\u3068\u306b\u3064\u306a\u304c\u308a\u307e\u3059\u3002<\/p>\n<p>\u305f\u304b\u304c\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u3001\u3055\u308c\u3069\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u3002\u30b9\u30c6\u30c3\u30d7\u6570\u306e\u591a\u3044\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306b\u3068\u3063\u3066\u3001\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u8a66\u85ac\u306e\u9078\u629e\u3082\u91cd\u8981\u306a\u8981\u7d20\u306e\u4e00\u3064\u306a\u306e\u3067\u3059\u3002<\/p>\n<h2>\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u8a66\u85ac\u306e\u9078\u3073\u65b9<\/h2>\n<p>\u3055\u3066\u3001\u305d\u308c\u3067\u306f\u3001\u5b9f\u9a13\u306b\u9069\u5207\u306a\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u8a66\u85ac\u3092\u9078\u3093\u3067\u3044\u304d\u307e\u3057\u3087\u3046\u3002<\/p>\n<p>\u3057\u304b\u3057\u3001\u305d\u3046\u8a00\u308f\u308c\u3066\u3082\u3001\u5e02\u5834\u306b\u3042\u308b\u8a66\u85ac\u306e\u7a2e\u985e\u304c\u591a\u3059\u304e\u3066\u3001\u3069\u308c\u304b\u3089\u624b\u3092\u3064\u3051\u308c\u3070\u3088\u3044\u306e\u304b\u308f\u304b\u3089\u306a\u3044\u3067\u3059\u3088\u306d\u3002\u305d\u3053\u3067\u3001\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u8a66\u85ac\u306e\u9078\u629e\u30dd\u30a4\u30f3\u30c8\u3092\u307e\u305a\u306f3\u3064\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n<p><strong>\uff1c\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u8a66\u85ac \u9078\u629e\u306e\u30dd\u30a4\u30f3\u30c8\uff1e<\/strong><\/p>\n<ol>\n<li style=\"list-style-type: none\">\n<ol>\n<li>\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u52b9\u679c\u304c\u9ad8\u304f\u3001\u304b\u3064\u6c4e\u7528\u6027\u304c\u9ad8\u3044\u3068\u3055\u308c\u3066\u3044\u308b\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u8a66\u85ac\u304b\u3089\u9806\u306b\u8a66\u3057\u3066\u3044\u304f<\/li>\n<li>\u30bf\u30fc\u30b2\u30c3\u30c8\u3084\u691c\u51fa\u306b\u91cd\u8981\u306a\u7269\u8cea\u304c\u542b\u307e\u308c\u3066\u3044\u308b\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u8a66\u85ac\u306f\u9078\u3070\u306a\u3044<\/li>\n<li>\u30ea\u30f3\u9178\u5316\u30bf\u30f3\u30d1\u30af\u8cea\u691c\u51fa\u306e\u5834\u5408\u3001PBS\u30d9\u30fc\u30b9\u3067\u306f\u306a\u304fTBS\u30d9\u30fc\u30b9\u306e\u8a66\u85ac\u3092\u9078\u3076<\/li>\n<\/ol>\n<\/li>\n<\/ol>\n<p>1\u306b\u3064\u3044\u3066\u306f\u3001\u8a66\u3057\u3066\u307f\u306a\u3051\u308c\u3070\u308f\u304b\u3089\u306a\u3044\u304b\u3089\u3053\u305d\u3001\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u52b9\u679c\u304c\u9ad8\u304f\u3001\u304b\u3064\u5e45\u5e83\u304f\u4f7f\u7528\u3067\u304d\u308b\u3082\u306e\u304c\u304a\u3059\u3059\u3081\u3067\u3059\u30022\u30683\u306f\u5b9f\u9a13\u7cfb\u306b\u3088\u3063\u3066\u300c\u4f7f\u7528NG\u306e\u7d44\u307f\u5408\u308f\u305b\u300d\u3092\u628a\u63e1\u3057\u3066\u304a\u3051\u3070\u554f\u984c\u3042\u308a\u307e\u305b\u3093\u3002\u30b5\u30fc\u30e2\u30d5\u30a3\u30c3\u30b7\u30e3\u30fc\u30b5\u30a4\u30a8\u30f3\u30c6\u30a3\u30d5\u30a3\u30c3\u30af\u306e\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u8a66\u85ac\u3092\u3001\u304a\u3059\u3059\u3081\u9806\u306b\u793a\u3057\u305f\u306e\u304c\u88681\u3067\u3059\u3002<\/p>\n<table>\n<tbody>\n<tr>\n<td style=\"text-align: center\" width=\"59\"><strong>\u304a\u3059\u3059\u3081\u9806<\/strong><\/td>\n<td style=\"text-align: center\" width=\"246\"><strong>\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u8a66\u85ac<\/strong><\/td>\n<td style=\"text-align: center\" width=\"310\"><strong>\u7279\u5fb4<\/strong><\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"59\">1<\/td>\n<td width=\"246\">StartingBlock Blocking Buffer<br \/>\n\uff08<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/37579?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_5_Social_LAB\" target=\"_blank\" rel=\"noopener\">TBS<\/a>\uff0f<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/37578?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_5_Social_LAB\" target=\"_blank\" rel=\"noopener\">PBS<\/a>\uff0f<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/37543?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_5_Social_LAB\" target=\"_blank\" rel=\"noopener\">TBS-T<\/a>\uff0f<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/37539?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_5_Social_LAB\" target=\"_blank\" rel=\"noopener\">PBS-T<\/a>\uff09<\/td>\n<td width=\"310\">\u7cbe\u88fd\u6e08\u307f\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u4f7f\u7528\u3001\u30d3\u30aa\u30c1\u30f3\u30d5\u30ea\u30fc\u3001\u30d2\u30c8\u8840\u6e05\u30d5\u30ea\u30fc\u3001Tween 20\u3092\u542b\u3080TBS-T\uff0fPBS-T\u30bf\u30a4\u30d7\u3082\u9078\u629e\u53ef<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"59\">2<\/td>\n<td width=\"246\">SuperBlock Blocking Buffer<br \/>\n\uff08<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/37581?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_5_Social_LAB\" target=\"_blank\" rel=\"noopener\">TBS<\/a>\uff0f<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/37580?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_5_Social_LAB\" target=\"_blank\" rel=\"noopener\">PBS<\/a>\uff0f<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/37536?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_5_Social_LAB\" target=\"_blank\" rel=\"noopener\">TBS-T<\/a>\uff0f<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/37516?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_5_Social_LAB\" target=\"_blank\" rel=\"noopener\">PBS-T<\/a>\uff09<\/td>\n<td width=\"310\">\u7cbe\u88fd\u6e08\u307f\u7cd6\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u4f7f\u7528\u3001\u30d3\u30aa\u30c1\u30f3\u30d5\u30ea\u30fc\u3001\u30d2\u30c8\u8840\u6e05\u30d5\u30ea\u30fc\u3001Tween 20\u3092\u542b\u3080TBS-T\uff0fPBS-T\u30bf\u30a4\u30d7\u3082\u9078\u629e\u53ef<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"59\">3<\/td>\n<td width=\"246\"><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/37527?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_5_Social_LAB\" target=\"_blank\" rel=\"noopener\">Fish Serum Blocking Buffer<\/a><\/td>\n<td width=\"310\">\u30b5\u30b1\u8840\u6e05\u7531\u6765\u3001\u54fa\u4e73\u985e\u30bf\u30f3\u30d1\u30af\u8cea\u30d5\u30ea\u30fc\u3001PBS\u30d9\u30fc\u30b9<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" rowspan=\"2\" width=\"59\">4<\/td>\n<td width=\"246\">Thermo Scientific\u2122<br \/>\nPierce\u2122 Protein-Free Blocking Buffer<br \/>\n<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/37585?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_5_Social_LAB\" target=\"_blank\" rel=\"noopener\">TBS<\/a>\uff0f<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/37584?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_5_Social_LAB\" target=\"_blank\" rel=\"noopener\">PBS<\/a>\uff0f<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/37571?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_5_Social_LAB\" target=\"_blank\" rel=\"noopener\">TBS-T<\/a>\uff0f<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/37573?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_5_Social_LAB\" target=\"_blank\" rel=\"noopener\">PBS-T<\/a><\/td>\n<td width=\"310\">\u30bf\u30f3\u30d1\u30af\u8cea\u30d5\u30ea\u30fc\u3001\u30d3\u30aa\u30c1\u30f3\u30d5\u30ea\u30fc\u3001Tween20\u3092\u542b\u3080TBS-T\uff0fPBS-T\u30bf\u30a4\u30d7\u3082\u9078\u629e\u53ef<\/td>\n<\/tr>\n<tr>\n<td width=\"246\">Thermo Scientific\u2122<br \/>\n<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/jp\/ja\/37576?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_5_Social_LAB\" target=\"_blank\" rel=\"noopener\">Pierce\u2122 Fast Blocking Buffer<\/a><\/td>\n<td width=\"310\">\u308f\u305a\u304b5\u5206\u3067\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u304c\u5b8c\u4e86\u3001TBS-T\u30d9\u30fc\u30b9<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" rowspan=\"5\" width=\"59\">\u00a05<\/td>\n<td width=\"246\">Thermo Scientific\u2122<br \/>\nBlocker\u2122 BSA<br \/>\n<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/37520?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_5_Social_LAB\" target=\"_blank\" rel=\"noopener\">TBS<\/a>\uff0f<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/37525?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_5_Social_LAB\" target=\"_blank\" rel=\"noopener\">PBS<\/a><\/td>\n<td width=\"310\">\u7cbe\u88fdBSA\u3092\u4f7f\u7528<\/td>\n<\/tr>\n<tr>\n<td width=\"246\">Thermo Scientific\u2122<br \/>\nBlocker\u2122 Casein<br \/>\n<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/37583?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_5_Social_LAB\" target=\"_blank\" rel=\"noopener\">TBS<\/a>\uff0f<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/37582?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_5_Social_LAB\" target=\"_blank\" rel=\"noopener\">PBS<\/a><\/td>\n<td width=\"310\">\u7cbe\u88fd\u30ab\u30bc\u30a4\u30f3\u3092\u4f7f\u7528<br \/>\n\u203b\u30ea\u30f3\u9178\u5316\u30bf\u30f3\u30d1\u30af\u8cea\u691c\u51fa\u306b\u306f\u304a\u3059\u3059\u3081\u3067\u304d\u307e\u305b\u3093<\/td>\n<\/tr>\n<tr>\n<td width=\"246\">Thermo Scientific\u2122<br \/>\n<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/37530?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_5_Social_LAB\" target=\"_blank\" rel=\"noopener\">Blocker\u2122 BLOTTO Blocking Buffer<\/a><\/td>\n<td width=\"310\">1\u500d\u6fc3\u5ea6\u3067\u3059\u3050\u306b\u4f7f\u7528\u3067\u304d\u308b\u30df\u30eb\u30af\u30d9\u30fc\u30b9\u306e\u8a66\u85ac<br \/>\n\u203b\u30a2\u30d3\u30b8\u30f3-\u30d3\u30aa\u30c1\u30f3\u7cfb\u306e\u691c\u51fa\u306b\u306f\u304a\u3059\u3059\u3081\u3067\u304d\u307e\u305b\u3093<\/td>\n<\/tr>\n<tr>\n<td width=\"246\">Thermo Scientific\u2122<br \/>\n<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/37587?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_5_Social_LAB\" target=\"_blank\" rel=\"noopener\">Pierce\u2122 Clear Milk Blocking Buffer<\/a><\/td>\n<td width=\"310\">\u30b9\u30ad\u30e0\u30df\u30eb\u30af\u304b\u3089\u306e\u5207\u308a\u66ff\u3048\u306b\u9069\u3057\u305f\u30d0\u30c3\u30d5\u30a1\u30fc<br \/>\n\u203b\u30a2\u30d3\u30b8\u30f3-\u30d3\u30aa\u30c1\u30f3\u7cfb\u306e\u691c\u51fa\u306b\u306f\u304a\u3059\u3059\u3081\u3067\u304d\u307e\u305b\u3093<\/td>\n<\/tr>\n<tr>\n<td width=\"246\">Thermo Scientific\u2122<br \/>\n<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/jp\/ja\/37565?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_5_Social_LAB\" target=\"_blank\" rel=\"noopener\">Blocker\u2122 FL Fluorescent Blocking Buffer<\/a><\/td>\n<td width=\"310\">\u86cd\u5149\u691c\u51fa\u6642\u306e\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9\u3092\u4f4e\u6e1b<br \/>\n\uff08\u5316\u5b66\u767a\u5149\u3067\u3082\u4f7f\u7528\u53ef\uff09<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>\u30d5\u30a1\u30fc\u30b9\u30c8\u30c1\u30e7\u30a4\u30b9\u3068\u3057\u3066\u304a\u3059\u3059\u3081\u3057\u3066\u3044\u308b\u306e\u306f\u3001StartingBlock Blocking Buffer\u3067\u3059\u3002\u7cbe\u88fd\u6e08\u307f\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u4f7f\u7528\u3057\u305f\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u8a66\u85ac\u3067\u3001\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u52b9\u679c\u304c\u9ad8\u304f\u3001\u304b\u3064\u5e45\u5e83\u3044\u5b9f\u9a13\u7cfb\u306b\u5bfe\u5fdc\u3057\u3066\u3044\u308b\u305f\u3081\u3067\u3059\u3002\u3082\u3057StartingBlock Blocking Buffer\u3067\u826f\u597d\u306a\u7d50\u679c\u304c\u5f97\u3089\u308c\u306a\u3044\u5834\u5408\u306f\u3001Thermo Scientific\u2122 SuperBlock\u2122 Blocking Buffer\u3001\u6b21\u306bFish Serum Blocking Buffer\u2026\u306e\u3088\u3046\u306b\u3001\u88681\u306e1\u21922\u21923\u21924\u21925\u306e\u9806\u3067\u306e\u3054\u8a66\u7528\u3092\u304a\u3059\u3059\u3081\u3057\u307e\u3059\u3002<\/p>\n<p>\u306a\u304a\u6ce8\u610f\u70b9\u3068\u3057\u3066\u3001\u30ea\u30f3\u9178\u5316\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u691c\u51fa\u3059\u308b\u5834\u5408\u306f\u3001PBS\u3092\u30d9\u30fc\u30b9\u3068\u3057\u305f\u8a66\u85ac\u306f\u907f\u3051\u3066\u304f\u3060\u3055\u3044\u3002\u30ab\u30bc\u30a4\u30f3\u306e\u4f7f\u7528\u3082\u304a\u3059\u3059\u3081\u3067\u304d\u307e\u305b\u3093\u3002\u307e\u305f\u30df\u30eb\u30af\u30d9\u30fc\u30b9\u306e\u8a66\u85ac\u306f\u3001\u30d3\u30aa\u30c1\u30f3\u3092\u542b\u3093\u3067\u3044\u308b\u305f\u3081\u3001\u30a2\u30d3\u30b8\u30f3-\u30d3\u30aa\u30c1\u30f3\u7cfb\u306b\u306f\u4f7f\u7528\u3067\u304d\u307e\u305b\u3093\u306e\u3067\u3001\u3054\u6ce8\u610f\u304f\u3060\u3055\u3044\u3002<\/p>\n<p>\u307e\u305f\u3001\u300cTBS\u300d\u3084\u300cPBS\u300d\u306a\u3069\u306e\u8868\u8a18\u306f\u3001\u305d\u306e\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u8a66\u85ac\u304c\u3069\u3093\u306a\u30d0\u30c3\u30d5\u30a1\u30fc\u306b\u6eb6\u3051\u3066\u3044\u308b\u304b\u3092\u793a\u3059\u3082\u306e\u3067\u3059\u3002\u3053\u306e\u3046\u3061\u3001TBS-T\u3084PBS-T\u306f\u3001\u754c\u9762\u6d3b\u6027\u5264Tween\u2122 20\u3092\u542b\u3093\u3067\u3044\u308bTBS\u3084PBS\u3067\u3001Tween 20\u306b\u306f\u975e\u7279\u7570\u53cd\u5fdc\u3092\u6291\u3048\u308b\u52b9\u679c\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<h2>\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u306e\u6761\u4ef6\u691c\u8a0e<\/h2>\n<p>\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u8a66\u85ac\u304c\u6c7a\u307e\u3063\u305f\u3089\u3001\u5b9f\u9a13\u6761\u4ef6\u3092\u6c7a\u3081\u307e\u3057\u3087\u3046\u3002<\/p>\n<p>\u307e\u305a\u306f\u9078\u3093\u3060\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u8a66\u85ac\u306e\u63a8\u5968\u6761\u4ef6\uff08\u6fc3\u5ea6\u3001\u6e29\u5ea6\u3001\u6642\u9593\uff09\u3067\u8a66\u3057\u3066\u307f\u3066\u304f\u3060\u3055\u3044\u3002\u4f8b\u3048\u3070StartingBlock Blocking Buffer\u306e\u5834\u5408\u3001\u8ee2\u5199\u5f8c\u306e\u30e1\u30f3\u30d6\u30ec\u30f3\u30925\u5206\u9593\u84b8\u7559\u6c34\u3067\u6d17\u6d44\u3057\u305f\u5f8c\u3001\u30e1\u30f3\u30d6\u30ec\u30f3\u304c\u5341\u5206\u6d78\u308b\u91cf\u306e StartingBlock Blocking Buffer\u3092\u6dfb\u52a0\u3057\u300115\uff5e30\u5206\u3001\u5e38\u6e29\u3067\u632f\u3068\u3046\u3057\u307e\u3059\u3002\u307e\u305f\u3001\u4f8b\u3048\u3070\u5e02\u8ca9\u85ac\u3067\u306f\u306a\u3044\u30b9\u30ad\u30e0\u30df\u30eb\u30af\u306e\u5834\u5408\u306f\u30015%\u3067\u5ba4\u6e291\u6642\u9593\u304b\u30014 \u2103\u3067\u30aa\u30fc\u30d0\u30fc\u30ca\u30a4\u30c8\u306e\u6761\u4ef6\u304c\u3088\u304f\u4f7f\u308f\u308c\u307e\u3059\u3002<\/p>\n<p>\u3082\u3057\u3082\u63a8\u5968\u6761\u4ef6\u3067\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u304c\u4e0d\u5341\u5206\u306a\u3089\u3001\u624b\u3063\u53d6\u308a\u65e9\u3044\u306e\u306f\u6642\u9593\u306e\u5ef6\u9577\u3067\u3059\u3002\u4f8b\u3048\u3070StartingBlock Blocking Buffer\u306e\u5834\u5408\u3001\u6700\u5927\u306e30\u5206\u307e\u3067\u5ef6\u9577\u3057\u307e\u3059\u3002\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u6642\u9593\u3092\u9577\u304f\u3059\u308b\u3053\u3068\u3067\u3001\u52b9\u679c\u3092\u9ad8\u3081\u3089\u308c\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<p>\u3057\u304b\u3057\u3001\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u6761\u4ef6\u3084\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u8a66\u85ac\u306e\u691c\u8a0e\u3060\u3051\u3067\u306f\u9650\u5ea6\u304c\u3042\u308b\u3053\u3068\u3092\u77e5\u3063\u3066\u304a\u304d\u307e\u3057\u3087\u3046\u3002\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9\u306b\u5f71\u97ff\u3059\u308b\u306e\u306f\u3001\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u8a66\u85ac\u4ee5\u5916\u306b\u3082\u3001\u9175\u7d20\u304c\u6a19\u8b58\u3055\u308c\u305f\u4e8c\u6b21\u6297\u4f53\u6fc3\u5ea6\u306a\u3069\u304c\u3042\u308a\u307e\u3059\u3002<b>\u4e8c\u6b21\u6297\u4f53\u6fc3\u5ea6\u304c\u9ad8\u3059\u304e\u308b\u5834\u5408\u3001\u3044\u304f\u3089\u5b9f\u9a13\u306b\u5408\u3063\u305f\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u8a66\u85ac\u3092\u4f7f\u3063\u3066\u3082\u3001\u6642\u9593\u3092\u5ef6\u3070\u3057\u3066\u3082\u3001\u6539\u5584\u3057\u306a\u3044\u3053\u3068\u3082\u591a\u3044\u3067\u3059\u3002<\/b>\u305d\u306e\u305f\u3081\u3001\u4f8b\u3048\u3070StartingBlock Blocking Buffer\u3092\u4f7f\u7528\u3057\u3066\u3082\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9\u304c\u771f\u3063\u9ed2\u3067\u307e\u3063\u305f\u304f\u30d0\u30f3\u30c9\u304c\u898b\u3048\u306a\u3044\u5834\u5408\u306f\u3001\u4e8c\u6b21\u6297\u4f53\u306e\u5e0c\u91c8\u3082\u691c\u8a0e\u3057\u307e\u3057\u3087\u3046\u3002<\/p>\n<p>\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u691c\u51fa\u306b\u306f\u3001\u6cf3\u52d5\u6642\u306e\u30b5\u30f3\u30d7\u30eb\u6dfb\u52a0\u91cf\u3001\u8ee2\u5199\u3001\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u3001\u6297\u4f53\u53cd\u5fdc\u3001\u6d17\u6d44\u6761\u4ef6\u3001\u691c\u51fa\u8a66\u85ac\u306e\u611f\u5ea6\u306a\u3069\u3055\u307e\u3056\u307e\u306a\u30d5\u30a1\u30af\u30bf\u30fc\u304c\u7d61\u307f\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u3001\u3046\u307e\u304f\u3044\u304b\u306a\u3044\u3068\u304d\u306f\u3001\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u306e\u307f\u306b\u3053\u3060\u308f\u3089\u305a\u3001\u6cf3\u52d5\u304b\u3089\u691c\u51fa\u307e\u3067\u306e\u4e00\u9023\u306e\u6d41\u308c\u306e\u4e2d\u3067\u8003\u3048\u3089\u308c\u308b\u539f\u56e0\u3092\u30d4\u30c3\u30af\u30a2\u30c3\u30d7\u3057\u3001\u5bfe\u7b56\u3092\u8b1b\u3058\u308b\u3053\u3068\u304c\u5927\u5207\u3067\u3059\u3002<\/p>\n<h2>\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u8a66\u85ac\u306e\u9078\u3073\u65b9<\/h2>\n<p>\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u8a66\u85ac\u306e\u9078\u3073\u65b9\u306b\u3064\u3044\u3066\u3001\u307e\u3068\u3081\u3067\u3059\u3002<\/p>\n<ul>\n<li>\u5b9f\u9a13\u306b\u9069\u3057\u305f\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u8a66\u85ac\u3092\u9078\u3076\u3053\u3068\u3067\u3001\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9\u3092\u6291\u3048\u3001\u3088\u308a\u5fae\u91cf\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u691c\u51fa\u3067\u304d\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002<\/li>\n<li>\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u8a66\u85ac\u306f\u3001\u307e\u305a\u306f\u52b9\u679c\u3084\u6c4e\u7528\u6027\u304c\u9ad8\u3044\u3082\u306e\u3092\u9078\u3073\u307e\u3057\u3087\u3046\u3002\u307e\u305f\u3001\u30ab\u30bc\u30a4\u30f3\u306f\u30ea\u30f3\u9178\u5316\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u3001\u30b9\u30ad\u30e0\u30df\u30eb\u30af\u4e2d\u306b\u306f\u30d3\u30aa\u30c1\u30f3\u304c\u542b\u307e\u308c\u3066\u3044\u307e\u3059\u306e\u3067\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u691c\u51fa\u306b\u5f71\u97ff\u3057\u306a\u3044\u8a66\u85ac\u3092\u9078\u3073\u307e\u3057\u3087\u3046\u3002<\/li>\n<li>\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u8a66\u85ac\u3060\u3051\u3067\u306f\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9\u306e\u8efd\u6e1b\u306b\u9650\u5ea6\u304c\u3042\u308a\u307e\u3059\u3002\u30c8\u30e9\u30d6\u30eb\u6642\u306f\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u4e00\u9023\u306e\u6d41\u308c\u306e\u4e2d\u3067\u539f\u56e0\u3092\u8003\u3048\u307e\u3057\u3087\u3046\u3002<\/li>\n<\/ul>\n<p><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/western-blot-protein-ts-6\/?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_5_Social_LAB\" rel=\"noopener\" target=\"_blank\">\u6b21\u56de<\/a>\u306f\u3001\u6297\u4f53\u9078\u629e\u306e\u30dd\u30a4\u30f3\u30c8\u306b\u3064\u3044\u3066\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n<p><b>\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u88fd\u54c1\u9078\u3073\u306e\u30dd\u30a4\u30f3\u30c8\u30b7\u30ea\u30fc\u30ba\u306f\u3053\u3061\u3089\uff1a<\/b><br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/western-blot-protein-ts-1\/?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_5_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u88fd\u54c1\u9078\u3073\u306b\u3082\u3046\u56f0\u3089\u306a\u3044\uff01\u7b2c1\u56de\uff1a\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u62bd\u51fa\u65b9\u6cd5<\/a><br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/western-blot-protein-ts-2\/?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_5_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u88fd\u54c1\u9078\u3073\u306b\u3082\u3046\u56f0\u3089\u306a\u3044\uff01\u7b2c2\u56de\uff1a\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u30ad\u30c3\u30c8<\/a><br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/western-blot-protein-ts-3\/?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_5_Social_LAB\" target=\"_blank\" rel=\"noopener\">SDS-PAGE\u306e\u9078\u3073\u65b9\uff1a\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u88fd\u54c1\u9078\u3073\u306b\u3082\u3046\u56f0\u3089\u306a\u3044\uff01\u7b2c3\u56de<\/a><br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/western-blot-protein-ts-4\/?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_5_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u8ee2\u5199\u6761\u4ef6\u306e\u9078\u3073\u65b9\uff1a\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u88fd\u54c1\u9078\u3073\u306b\u3082\u3046\u56f0\u3089\u306a\u3044\uff01\u7b2c4\u56de<\/a><br \/>\n\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u8a66\u85ac\u306e\u30dd\u30a4\u30f3\u30c8\uff1a\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u88fd\u54c1\u9078\u3073\u306b\u3082\u3046\u56f0\u3089\u306a\u3044\uff01\u7b2c5\u56de<br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/western-blot-protein-ts-6\/?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_5_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u6297\u4f53\u3092\u9078\u3076\u3068\u304d\u306b\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\u3053\u3068\uff1a\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u88fd\u54c1\u9078\u3073\u7b2c6\u56de<\/a><br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/western-blot-protein-ts-7\/?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_5_Social_LAB\" rel=\"noopener\" target=\"_blank\">\u5316\u5b66\u767a\u5149\u3068\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u691c\u51fa\u306e\u9078\u629e\u30dd\u30a4\u30f3\u30c8\uff1a\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u9078\u3073\u65b9\u3000\u7b2c7\u56de<\/a><\/p>\n<h3>\u3010\u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3011\u30bf\u30f3\u30d1\u30af\u8cea\u89e3\u6790\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u30cf\u30f3\u30c9\u30d6\u30c3\u30af<\/h3>\n<p>\u52b9\u7387\u7684\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u62bd\u51fa\u304b\u3089\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u89e3\u6790\u30c4\u30fc\u30eb\u307e\u3067\u3001\u5305\u62ec\u7684\u306b\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u3092\u7d39\u4ecb\u3057\u3066\u304a\u308a\u307e\u3059\u3002PDF\u30d5\u30a1\u30a4\u30eb\u306e\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3092\u3054\u5e0c\u671b\u306e\u65b9\u306f\u3001\u4e0b\u8a18\u30dc\u30bf\u30f3\u3088\u308a\u304a\u7533\u3057\u8fbc\u307f\u304f\u3060\u3055\u3044\u3002<\/p>\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/catalogs-protein-form.html?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_5_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/p>\n<h2>\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u691c\u51fa\u30fb\u89e3\u6790\u95a2\u9023\u3001\u305d\u306e\u4ed6\u306e\u8a18\u4e8b\u306f\u3053\u3061\u3089<\/h2>\n<table style=\"border-color: lightgray;border-collapse: collapse;padding: 20px\" border=\"1\">\n<tbody>\n<tr>\n<td style=\"border-color: lightgray;padding: 20px\">\n<ul>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic10\/?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_5_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u7b2c10\u56de\u301110\u5206\u3067\u5206\u304b\u308b\u30d3\u30aa\u30c1\u30f3\u7d50\u5408\u30bf\u30f3\u30d1\u30af\u8cea\u307e\u3068\u3081\n<p><\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic11\/?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_5_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u7b2c11\u56de\u3011\u30d3\u30aa\u30c1\u30f3\u6a19\u8b58\u8a66\u85ac\u307e\u3068\u3081\n<p><\/a><\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>Tween is a trademark of Croda Americas LLC.<br 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