{"id":153,"date":"2020-03-04T00:00:00","date_gmt":"2020-03-03T15:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic12\/"},"modified":"2025-06-05T17:48:11","modified_gmt":"2025-06-05T08:48:11","slug":"protein-basic12","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic12\/","title":{"rendered":"\u3055\u307e\u3056\u307e\u306a\u6297\u4f53\u7cbe\u88fd\u6cd5\u3092\u307e\u3068\u3081\u3066\u307f\u305f \u305d\u306e1\uff5c\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\uff01\u30bf\u30f3\u30d1\u30af\u8cea\u5b9f\u9a13\u3042\u308c\u3053\u308c \u7b2c12\u56de"},"content":{"rendered":"<h2>\u306f\u3058\u3081\u306b<\/h2>\n<p>\u6297\u4f53\u306f\u3001\u6297\u539f\u3092\u514d\u75ab\u3057\u305f\u52d5\u7269\u306e\u8840\u6e05\uff08\u30dd\u30ea\u30af\u30ed\u30fc\u30ca\u30eb\u6297\u4f53\uff09\u3084\u8179\u6c34\u3001\u3042\u308b\u3044\u306f\u30cf\u30a4\u30d6\u30ea\u30c9\u30fc\u30de\u7d30\u80de\u57f9\u990a\u4e0a\u6e05\uff08\u30e2\u30ce\u30af\u30ed\u30fc\u30ca\u30eb\u6297\u4f53\uff09\u304b\u3089\u7cbe\u88fd\u3057\u307e\u3059\u3002\u7cbe\u88fd\u65b9\u6cd5\u306b\u306f\u3001\u5927\u304d\u304f\u5206\u3051\u30663\u3064\u306e\u30ec\u30d9\u30eb\u3001\u3059\u306a\u308f\u3061\u7c97\u7cbe\u88fd\u7a0b\u5ea6\u306e\u65b9\u6cd5\uff08Crude\u30ec\u30d9\u30eb\u306e\u7cbe\u88fd\uff09\u3001\u6297\u4f53\u30af\u30e9\u30b9\uff08\u307e\u305f\u306f\u30a2\u30a4\u30bd\u30bf\u30a4\u30d7\uff09\u306e\u30ec\u30d9\u30eb\u3067\u7cbe\u88fd\u3059\u308b\u65b9\u6cd5\uff08\u6297\u4f53\u30af\u30e9\u30b9\u30ec\u30d9\u30eb\u306e\u7cbe\u88fd\uff09\u3001\u76ee\u7684\u6297\u539f\u306b\u7279\u7570\u7684\u306a\u6297\u4f53\u7cbe\u88fd\u3092\u884c\u3046\u65b9\u6cd5\uff08Specific\u306a\u7cbe\u88fd\uff09\u304c\u3042\u308a\u3001\u3055\u307e\u3056\u307e\u306a\u65b9\u6cd5\u304c\u5229\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>\u4eca\u56de\u306f\u305d\u308c\u305e\u308c\u306e\u30ec\u30d9\u30eb\u3054\u3068\u306b\u7cbe\u88fd\u65b9\u6cd5\u3092\u6319\u3052\u3066\u305d\u306e\u6982\u8981\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\uff01\uff08Specific\u306a\u7cbe\u88fd\u306b\u3064\u3044\u3066\u306f\u6b21\u56de\u63b2\u8f09\u306e\u4e88\u5b9a\u3067\u3059\uff09\u3002<\/p>\n<h2>Crude\u30ec\u30d9\u30eb\u306e\u7cbe\u88fd<\/h2>\n<p>\u5869\u3092\u52a0\u3048\u308b\u3053\u3068\u3067\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u6eb6\u89e3\u5ea6\u3092\u4e0b\u3052\u3066\u6c88\u6bbf\u3055\u305b\u308b\u5869\u6790\u6cd5\u304c\u3053\u306e\u30ec\u30d9\u30eb\u306e\u7cbe\u88fd\u306b\u4f7f\u7528\u3055\u308c\u307e\u3059\u3002<strong>\u5869\u6790\u3067\u6700\u3082\u5e83\u304f\u5229\u7528\u3055\u308c\u3066\u3044\u308b\u306e\u304c\u3001\u5869\u306e\u4e2d\u3067\u3082\u6eb6\u89e3\u5ea6\u306e\u9ad8\u3044\u786b\u9178\u30a2\u30f3\u30e2\u30cb\u30a6\u30e0\uff08\u4ee5\u4e0b\u3001\u786b\u5b89\uff09\u3067\u3059\u3002<\/strong>\u786b\u5b89\u306b\u3088\u308a\u6c88\u6bbf\u3057\u305f\u30bf\u30f3\u30d1\u30af\u8cea\u306f\u5909\u6027\u304c\u307b\u3068\u3093\u3069\u8d77\u3053\u3089\u305a\u5bb9\u6613\u306b\u518d\u6eb6\u89e3\u3067\u304d\u307e\u3059\u3002\u307e\u305f\u786b\u5b89\u4e2d\u3067\u306f\u30d7\u30ed\u30c6\u30a2\u30fc\u30bc\u306b\u3088\u308b\u5206\u89e3\u3084\u5fae\u751f\u7269\u306e\u5897\u6b96\u304c\u8d77\u304d\u306b\u304f\u3044\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>\u786b\u5b89\u3092\u7528\u3044\u305f\u5869\u6790\u306b\u3088\u308b\u5206\u753b\u306f\u3001\u786b\u5b89\u6c88\u6bbf\uff08\u307e\u305f\u306f\u786b\u5b89\u5206\u753b\uff09\u3068\u547c\u3070\u308c\u3066\u3044\u307e\u3059\u3002<strong>\u30a4\u30e0\u30ce\u30b0\u30ed\u30d6\u30ea\u30f3\u306e\u786b\u5b89\u5206\u753b\u3067\u306f\u3001\u4e00\u822c\u7684\u306b\u786b\u5b89\u309235-60%\u98fd\u548c\u306e\u6eb6\u6db2\u306b\u3057\u307e\u3059\u3002<\/strong>\u30bf\u30f3\u30d1\u30af\u8cea\u6eb6\u6db2\u306b\u786b\u5b89\u3092\u6dfb\u52a0\u3059\u308b\u969b\u306f\u3001\u652a\u62cc\u3057\u306a\u304c\u3089\u5c11\u91cf\u305a\u3064\u6dfb\u52a0\u3057\u307e\u3059\uff08\u4e00\u5ea6\u306b\u6dfb\u52a0\u3059\u308b\u3068\u5fc5\u8981\u4ee5\u4e0a\u306e\u6c88\u6bbf\u5316\u3092\u8d77\u3053\u3057\u3066\u3057\u307e\u3044\u307e\u3059\uff09\u3002\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u6c88\u6bbf\u3092\u9069\u5f53\u306a\u30d0\u30c3\u30d5\u30a1\u30fc\u3067\u6eb6\u89e3\u3057\u305f\u5f8c\u3001\u900f\u6790\u306a\u3069\u3067\u786b\u5b89\u3092\u9664\u304d\u307e\u3059\u3002<\/p>\n<h2>\u6297\u4f53\u30af\u30e9\u30b9\u30ec\u30d9\u30eb\u306e\u7cbe\u88fd<\/h2>\n<h3>\u6297\u4f53\u7d50\u5408\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u7528\u3044\u305f\u7cbe\u88fd<\/h3>\n<p><strong>\u540c\u3058\u6297\u4f53\u30af\u30e9\u30b9\u306b\u304a\u3044\u3066\u6297\u4f53\u306e\u69cb\u9020\u306f\u3088\u304f\u4f3c\u3066\u3044\u308b\u305f\u3081\u3001\u9069\u5f53\u306a\u30ea\u30ac\u30f3\u30c9\u3092\u7528\u3044\u308b\u3053\u3068\u3067\u540c\u3058\u30af\u30e9\u30b9\u306e\u6297\u4f53\u3092\u307e\u3068\u3081\u3066\u30a2\u30d5\u30a3\u30cb\u30c6\u30a3\u30fc\u7cbe\u88fd\u304c\u3067\u304d\u307e\u3059\u3002<\/strong>\u7d30\u83cc\u4e2d\u306b\u5b58\u5728\u3059\u308bProtein A\u3001Protein G\u3001Protein L\u306f\u3001\u6297\u4f53\uff08\u4e3b\u306bIgG\uff09\u3068\u7279\u7570\u7684\u306b\u7d50\u5408\u3059\u308b\u4ee3\u8868\u7684\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u3059\u3002\u3053\u308c\u3089\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u306f\u3001\u3044\u305a\u308c\u3082\u6297\u4f53\u306e\u6297\u539f\u7d50\u5408\u30c9\u30e1\u30a4\u30f3\u3068\u306f\u7d50\u5408\u305b\u305a\u3001Protein A\u3084Protein G\u306f\u6297\u4f53\u306eFc\u9818\u57df\u3068\u7d50\u5408\u3001Protein L\u306f\u6297\u4f53\u306e\u8efd\u9396\uff08\u03ba\u8efd\u9396\uff09\u3068\u7d50\u5408\u3057\u307e\u3059\u3002Protein A\u3001G\u3001L\u306f\u305d\u308c\u305e\u308c\u6297\u4f53\u306e\u52d5\u7269\u7a2e\u3084\u30b5\u30d6\u30af\u30e9\u30b9\u306b\u3088\u3063\u3066\u7d50\u5408\u30a2\u30d5\u30a3\u30cb\u30c6\u30a3\u30fc\u304c\u7570\u306a\u308b\u305f\u3081\u3001\u76ee\u7684\u30b5\u30f3\u30d7\u30eb\u3084\u76ee\u7684\u6297\u4f53\u306e\u30bf\u30a4\u30d7\u304b\u3089\u9069\u5f53\u306a\u6297\u4f53\u7d50\u5408\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u9078\u629e\u3057\u307e\u3059\u3002\u907a\u4f1d\u5b50\u5de5\u5b66\u7684\u624b\u6cd5\u306b\u3088\u308aProtein A\u3068Protein G\u306e\u6297\u4f53\u7d50\u5408\u30c9\u30e1\u30a4\u30f3\u540c\u58eb\u3092\u878d\u5408\u3057\u305fProtein A\/G\u3092\u7528\u3044\u305f\u5834\u5408\u3001Protein A\u3001G\u305d\u308c\u305e\u308c\u5358\u72ec\u306e\u5834\u5408\u306b\u6bd4\u3079\u3066\u3001\u5229\u7528\u3067\u304d\u308b\u6297\u4f53\u30bf\u30a4\u30d7\u306e\u5e45\u304c\u5e83\u304f\u306a\u308a\u307e\u3059\u3002<\/p>\n<div id=\"attachment_490\" style=\"width: 2538px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/protein-basic12-table1.png\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-490\" class=\"wp-image-490 size-full\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/protein-basic12-table1.png\" alt=\"protein-basic12-table1\" width=\"2528\" height=\"1586\" \/><\/a><p id=\"caption-attachment-490\" class=\"wp-caption-text\">\u88681\uff1a \u6297\u4f53\u30bf\u30a4\u30d7\u3068\u6297\u4f53\u7d50\u5408\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u7d50\u5408\u5f37\u5ea6<\/p><\/div>\n<p>\u6297\u4f53\u306e\u30a2\u30d5\u30a3\u30cb\u30c6\u30a3\u30fc\u7cbe\u88fd\u306b\u306f\u3001\u901a\u5e38\u3001\u3053\u308c\u3089\u306e\u6297\u4f53\u7d50\u5408\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u56fa\u5b9a\u5316\u3057\u305f\u30a2\u30ac\u30ed\u30fc\u30b9\u30ec\u30b8\u30f3\u3001\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c9\u30ec\u30b8\u30f3\u3001\u78c1\u6027\u30d3\u30fc\u30ba\u3092\u4f7f\u7528\u3057\u307e\u3059\u3002\u9069\u5f53\u306a\u7d50\u5408\u30d0\u30c3\u30d5\u30a1\u30fc\u3067\u5e73\u8861\u5316\u3057\u305f\u6297\u4f53\u7d50\u5408\u30bf\u30f3\u30d1\u30af\u8cea\u56fa\u5b9a\u5316\u30ec\u30b8\u30f3\u306b\u6297\u4f53\u3092\u542b\u3080\u30b5\u30f3\u30d7\u30eb\u6eb6\u6db2\u3092\u6dfb\u52a0\u3057\u3001\u6d17\u6d44\u5f8c\u3001\u6eb6\u51fa\u30d0\u30c3\u30d5\u30a1\u30fc\u3067\u51e6\u7406\u3057\u3066\u6297\u4f53\u3092\u7cbe\u88fd\u3057\u307e\u3059\u3002\u7d50\u5408\u306e\u81f3\u9069pH\u306f\u5404\u6297\u4f53\u7d50\u5408\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u7570\u306a\u308b\uff08Protein A\u306e\u81f3\u9069pH: 8.2\u3001Protein G\u306e\u81f3\u9069pH: 5\u3001Protein L\u306e\u81f3\u9069pH: 7.5\u3001Protein A\/G\u306e\u81f3\u9069pH: 5-8.2\uff09\u305f\u3081\u3001\u9069\u5f53\u306a\u30d0\u30c3\u30d5\u30a1\u30fc\u3092\u9078\u629e\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u6eb6\u51fa\u306b\u306f\u3001\u6297\u4f53\u7d50\u5408\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u306e\u7d50\u5408pH\u304b\u3089\u5927\u304d\u304f\u96e2\u308c\u305fpH\uff08\u9178\u6027\u307e\u305f\u306f\u5869\u57fa\u6027\uff09\u306b\u3088\u308b\u6eb6\u51fa\u3001\u9ad8\u5869\u6fc3\u5ea6\u306b\u3088\u308b\u6eb6\u51fa\u3001\u754c\u9762\u6d3b\u6027\u5264\u306a\u3069\u306e\u5909\u6027\u5264\u306b\u3088\u308b\u6eb6\u51fa\u3001\u7af6\u5408\u30ea\u30ac\u30f3\u30c9\u306b\u3088\u308b\u6eb6\u51fa\u304c\u5229\u7528\u3067\u304d\u307e\u3059\u3002\u4e00\u822c\u7684\u306b\u306f\u9178\u6027\u30d0\u30c3\u30d5\u30a1\u30fc\uff080.1 M glycine-HCl, pH 2-3\uff09\u306b\u3088\u308b\u6eb6\u51fa\u304c\u884c\u308f\u308c\u307e\u3059\u304c\u3001\u6297\u4f53\u306b\u3088\u3063\u3066\u306f\u9178\u306b\u3088\u308b\u5909\u6027\u3084\u51dd\u96c6\u3092\u5f15\u304d\u8d77\u3053\u3057\u3066\u3057\u307e\u3046\u305f\u3081\u6ce8\u610f\u304c\u5fc5\u8981\u3067\u3059\u3002<\/p>\n<h3>\u30cd\u30ac\u30c6\u30a3\u30d6\u30bb\u30ec\u30af\u30b7\u30e7\u30f3\u6cd5\u306b\u3088\u308b\u7cbe\u88fd<\/h3>\n<p><a href=\"http:\/\/www.thermofisher.com\/order\/catalog\/product\/45212?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa30_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Melon\u2122 Gel IgG Purification Kit<\/a>\u306f\u3001IgG\u6297\u4f53\u3092\u30cd\u30ac\u30c6\u30a3\u30d6\u30bb\u30ec\u30af\u30b7\u30e7\u30f3\u306b\u3088\u308a\u7cbe\u88fd\u3059\u308b\u305f\u3081\u306e\u30ec\u30b8\u30f3\u30b2\u30eb\u3067\u3059\u3002\u30b2\u30eb\u306b\u306f\u72ec\u81ea\u306e\u30ea\u30ac\u30f3\u30c9\u3092\u56fa\u5b9a\u5316\u3057\u3066\u304a\u308a\u3001\u8840\u6e05\u3001\u8179\u6c34\u3001\u57f9\u990a\u4e0a\u6e05\u306b\u542b\u307e\u308c\u308b\u3055\u307e\u3056\u307e\u306a\u30bf\u30f3\u30d1\u30af\u8cea<sup>\u2020<\/sup>\u3092\u4fdd\u6301\u3059\u308b\u3053\u3068\u304c\u51fa\u6765\u307e\u3059\u3002<strong>IgG\u306f\u7d50\u5408\u3057\u306a\u3044\u305f\u3081\u3001\u30b5\u30f3\u30d7\u30eb\u3092Melon Gel\u306b\u901a\u904e\u3055\u305b\u308b\u3068\u7d20\u901a\u308a\u753b\u5206\u306bIgG\u304c\u56de\u53ce\uff08\u30cd\u30ac\u30c6\u30a3\u30d6\u30bb\u30ec\u30af\u30b7\u30e7\u30f3\uff09\u3055\u308c\u307e\u3059\u3002<\/strong>IgG\u306e\u5178\u578b\u7684\u306a\u53ce\u7387\u306f90%\u4ee5\u4e0a\u3001\u7cbe\u88fd\u7d14\u5ea6\u306f80%\u4ee5\u4e0a\u3067\u3059\u3002Protein A\u306a\u3069\u306e\u6297\u4f53\u7d50\u5408\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u7570\u306a\u308a\u3001\u52d5\u7269\u7a2e\u3084\u30b5\u30d6\u30af\u30e9\u30b9\u3067\u307b\u3068\u3093\u3069\u5236\u9650\u3055\u308c\u305a<sup>\u2021<\/sup>\u3001\u6297\u4f53\u7d50\u5408\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u306e\u7d50\u5408\u30a2\u30d5\u30a3\u30cb\u30c6\u30a3\u30fc\u304c\u5f31\u3044IgG\u306e\u7cbe\u88fd\u306b\u3082\u5229\u7528\u53ef\u80fd\u3067\u3059\u3002\u6297\u4f53\u3092\u5909\u6027\u3055\u305b\u308b\u3088\u3046\u306a\u6eb6\u51fa\u30d0\u30c3\u30d5\u30a1\u30fc\u3092\u4f7f\u7528\u3059\u308b\u5fc5\u8981\u304c\u7121\u3044\u305f\u3081\u3001\u6297\u4f53\u306e\u6d3b\u6027\u3092\u4fdd\u6301\u3057\u305f\u307e\u307e\u7cbe\u88fd\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002\u4f7f\u7528\u6e08\u307f\u306e\u30b2\u30eb\u306f\u8907\u6570\u56de\u518d\u751f\u30fb\u518d\u5229\u7528\u3067\u304d\u307e\u3059\u3002<br \/>\n<span style=\"font-size: 8pt\"><sup>\u2020<\/sup> \u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30ea\u30f3\uff08\u7279\u306b\u30de\u30a6\u30b9\u3084\u30e9\u30c3\u30c8\u7531\u6765\uff09\u3068\u306e\u7d50\u5408\u30a2\u30d5\u30a3\u30cb\u30c6\u30a3\u30fc\u306f\u5f31\u3044\u305f\u3081\u3001\u6297\u4f53\u3068\u5171\u306b\u7d20\u901a\u308a\u753b\u5206\u306b\u56de\u53ce\u3055\u308c\u307e\u3059\u3002\u3042\u3089\u304b\u3058\u3081\u30b5\u30f3\u30d7\u30eb\u3092\u786b\u5b89\u5206\u753b\u3059\u308b\u3053\u3068\u3067\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30ea\u30f3\u3092\u542b\u307e\u306a\u3044\u6297\u4f53\u7cbe\u88fd\u304c\u53ef\u80fd\u3067\u3059\u3002<br \/>\n<sup>\u2021<\/sup> \u30c1\u30ad\u30f3IgY\u306e\u7cbe\u88fd\u306b\u306f\u5229\u7528\u3067\u304d\u307e\u305b\u3093\u3002\u30c1\u30ad\u30f3IgY\u306e\u7cbe\u88fd\u306b\u306f<a href=\"http:\/\/www.thermofisher.com\/order\/catalog\/product\/44916?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa30_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Thiophilic Adsorption Kit<\/a>\u307e\u305f\u306f<a href=\"http:\/\/www.thermofisher.com\/order\/catalog\/product\/44918?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa30_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Chicken IgY Purification Kit<\/a>\u304c\u304a\u3059\u3059\u3081\u3067\u3059\u3002<\/span><\/p>\n<div id=\"attachment_492\" style=\"width: 460px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/protein-basic12-fig1.jpg\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-492\" class=\"wp-image-492 size-full\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/protein-basic12-fig1.jpg\" alt=\"protein-basic12-fig1\" width=\"450\" height=\"255\" \/><\/a><p id=\"caption-attachment-492\" class=\"wp-caption-text\">\u56f31 Melon Gel\u307e\u305f\u306fProtein A\u56fa\u5b9a\u5316\u30ec\u30b8\u30f3\u3092\u7528\u3044\u305fIgG\u7cbe\u88fd \u30d2\u30c8\u3001\u30a6\u30b5\u30ae\u3001\u30e4\u30ae\u306e\u5404\u8840\u6e05\u30b5\u30f3\u30d7\u30eb\u3092\u3001\u305d\u308c\u305e\u308cMelon Gel\u307e\u305f\u306fProtein A\u56fa\u5b9a\u5316\u30ec\u30b8\u30f3\u3092\u7528\u3044\u3066\u7cbe\u88fd\u3057\u307e\u3057\u305f\u3002\u7cbe\u88fd\u5f8c\u306e\u30b5\u30f3\u30d7\u30eb\u3092SDS\u96fb\u6c17\u6cf3\u52d5\u306b\u3088\u308a\u5206\u96e2\u3057CBB\u67d3\u8272\uff08<a href=\"http:\/\/www.thermofisher.com\/order\/catalog\/product\/24590?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa30_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">GelCode\u2122 Blue Stain<\/a>\uff09\u306b\u3088\u308a\u691c\u51fa\u3057\u307e\u3057\u305f\u3002 S: \u8840\u6e05\u30b5\u30f3\u30d7\u30eb\u3001M: Melon Gel\u7d20\u901a\u308a\u753b\u5206\u3001A1\u304a\u3088\u3073A2: Protein A\u56fa\u5b9a\u5316\u30ec\u30b8\u30f3\u3092\u7528\u3044\u305f\u6eb6\u51fa\u753b\u5206 H: \u6297\u4f53\u91cd\u9396\uff08Heavy chain\uff09\u3001L: \u6297\u4f53\u8efd\u9396\uff08Light chain\uff09<\/p><\/div>\n<h3>Thiophilic Gel\u3092\u7528\u3044\u305f\u7cbe\u88fd<\/h3>\n<p>Thiophilic Gel\uff08T-Gel\u3068\u3082\u547c\u3070\u308c\u308b\uff09\u306f\u3001\u30b9\u30eb\u30db\u30f3\u57fa\u30fb\u30c1\u30aa\u30a8\u30fc\u30c6\u30eb\u57fa\u3092\u4fee\u98fe\u3057\u305f\u30b2\u30eb\uff08\u56f32\uff09\u3067\u3001\u975e\u30ab\u30aa\u30c8\u30ed\u30d4\u30c3\u30af\u5869\u306e\u6fc3\u5ea6\u304c\u9ad8\u3044\u3068thiophilic\u306a\u5438\u7740\u3084\u758e\u6c34\u7684\u306a\u5438\u7740\u304c\u8d77\u3053\u308a\u3084\u3059\u304f\u306a\u308a\u307e\u3059\u3002\u7279\u306b\u786b\u9178\u30ab\u30ea\u30a6\u30e0\u3084\u786b\u9178\u30a2\u30f3\u30e2\u30cb\u30a6\u30e0\u306e\u6fc3\u5ea6\u304c\u9ad8\u3044\u5834\u5408\u306bthiophilic\u306a\u5438\u7740\u304c\u8d77\u3053\u308a\u3084\u3059\u304f\u306a\u308a\u3001\u5869\u5316\u30ca\u30c8\u30ea\u30a6\u30e0\u306e\u6fc3\u5ea6\u304c\u9ad8\u3044\u5834\u5408\u306b\u758e\u6c34\u7684\u306a\u5438\u7740\u304c\u8d77\u3053\u308a\u3084\u3059\u304f\u306a\u308a\u307e\u3059\u3002<strong>thiophilic\u5438\u7740\u306f\u3001\u8840\u6e05\u3001\u8179\u6c34\u3001\u57f9\u990a\u7d30\u80de\u304b\u3089\u306e\u30a4\u30e0\u30ce\u30b0\u30ed\u30d6\u30ea\u30f3\u7cbe\u88fd\u306b\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002<\/strong><a href=\"http:\/\/www.thermofisher.com\/order\/catalog\/product\/44916?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa30_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Thiophilic Adsorption Kit<\/a>\u3067\u306f\u30010.5M\u306e\u786b\u9178\u30ab\u30ea\u30a6\u30e0\u3092\u542b\u3080Binding Buffer\uff08pH 8.0\uff09\u306b\u3088\u308a\u4e3b\u306bIgG\u3092\u7d50\u5408\u3001\u4f4e\u5869\u6fc3\u5ea6\u306eElution Buffer\uff08pH 7.4\uff09\u306b\u3088\u308a\u3001IgG\u3092\u7cbe\u88fd\u3059\u308b\u305f\u3081\u306e\u30ad\u30c3\u30c8\u3067\u3059\u3002thiophilic\u5438\u7740\u306f\u3001\u3055\u307e\u3056\u307e\u306a\u52d5\u7269\u7a2e\u3084\u30b5\u30d6\u30af\u30e9\u30b9\u306eIgG\u7cbe\u88fd\u306b\u5229\u7528\u3067\u304d\u3001\u30c1\u30ad\u30f3IgY\u306e\u7cbe\u88fd\u306b\u3082\u5229\u7528\u3067\u304d\u307e\u3059\u3002\u7a4f\u3084\u304b\u306a\u6761\u4ef6\u3067\u6eb6\u51fa\u3059\u308b\u305f\u3081\u3001\u6297\u4f53\u306e\u6d3b\u6027\u3092\u4fdd\u6301\u3057\u305f\u307e\u307e\u7cbe\u88fd\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002\u4f7f\u7528\u6e08\u307f\u306e\u30b2\u30eb\u306f\u8907\u6570\u56de\u518d\u751f\u30fb\u518d\u5229\u7528\u3067\u304d\u307e\u3059\u3002<\/p>\n<div id=\"attachment_493\" style=\"width: 410px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/protein-basic12-fig2.jpg\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-493\" class=\"size-full wp-image-493\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/protein-basic12-fig2.jpg\" alt=\"protein-basic12-fig2\" width=\"400\" height=\"121\" \/><\/a><p id=\"caption-attachment-493\" class=\"wp-caption-text\">\u56f32 Thiophilic Adsorbent\u306e\u69cb\u9020\uff08\u6a21\u5f0f\u56f3\uff09<\/p><\/div>\n<h3>\u30de\u30f3\u30ca\u30f3\u7d50\u5408\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u7528\u3044\u305fIgM\u7cbe\u88fd<\/h3>\n<p>\u30de\u30f3\u30ca\u30f3\u7d50\u5408\u30bf\u30f3\u30d1\u30af\u8cea\uff08mannan binding protein: MBP\uff09\u306f<strong>\u30de\u30f3\u30ce\u30fc\u30b9\u3084N-\u30a2\u30bb\u30c1\u30eb\u30b0\u30eb\u30b3\u30b5\u30df\u30f3\u306b\u7279\u7570\u7684\u306b\u7d50\u5408\u3059\u308b\u30ec\u30af\u30c1\u30f3\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u3059\u3002<\/strong><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/44897?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa30_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Pierce\u2122 IgM Purification Kit<\/a>\u306f\u3001IgM\u306e\u7cbe\u88fd\u306b\u81f3\u9069\u5316\u3057\u305fMBP\u56fa\u5b9a\u5316\u30ec\u30b8\u30f3\u3068\u5404\u7a2e\u30d0\u30c3\u30d5\u30a1\u30fc\u3092\u542b\u3080\u30ad\u30c3\u30c8\u3067\u3059\u3002IgM\u306e\u7d50\u5408\u306f\u6e29\u5ea6\u304a\u3088\u3073\u30ab\u30eb\u30b7\u30a6\u30e0\u6fc3\u5ea6\u4f9d\u5b58\u7684\u306b\u8d77\u3053\u308a\u307e\u3059\u3002\u7d50\u5408\u30d0\u30c3\u30d5\u30a1\u30fc\u306b\u306f20 mM\u306e\u5869\u5316\u30ab\u30eb\u30b7\u30a6\u30e0\u3092\u542b\u3080Tris-HCl\u30d0\u30c3\u30d5\u30a1\u30fc\uff08pH 7.4\uff09\u3092\u7528\u3044\u3066\u3001MBP\u56fa\u5b9a\u5316\u30ec\u30b8\u30f3\u306bIgM\u30924\u2103\u3067\u7d50\u5408\u3055\u305b\u307e\u3059\u3002\u6eb6\u51fa\u30d0\u30c3\u30d5\u30a1\u30fc\u306b\u306f\u7d50\u5408\u30d0\u30c3\u30d5\u30a1\u30fc\u304b\u3089\u5869\u5316\u30ab\u30eb\u30b7\u30a6\u30e0\u3092\u9664\u304d\u4ee3\u308f\u308a\u306bEDTA\u3092\u6dfb\u52a0\u3057\u305f\u30d0\u30c3\u30d5\u30a1\u30fc\u3092\u7528\u3044\u3066\u3001\u5ba4\u6e29\u3067\u6eb6\u51fa\u3057\u307e\u3059\u3002\u7a4f\u3084\u304b\u306a\u6761\u4ef6\u3067\u6eb6\u51fa\u3059\u308b\u305f\u3081\u3001\u6297\u4f53\u306e\u6d3b\u6027\u3092\u4fdd\u6301\u3057\u305f\u307e\u307e\u7cbe\u88fd\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/44897?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa30_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Pierce\u2122 IgM Purification Kit<\/a>\u306f\u3001\u7279\u306b\u30de\u30a6\u30b9\u8179\u6c34\u304b\u3089\u306eIgM\u7cbe\u88fd\u306b\u9069\u3057\u3066\u304a\u308a\u300190%\u4ee5\u4e0a\u306e\u7d14\u5ea6\u3067\u7cbe\u88fd\u53ef\u80fd\u3067\u3059\u3002\u4f7f\u7528\u6e08\u307f\u306e\u30b2\u30eb\u306f\u8907\u6570\u56de\u518d\u751f\u30fb\u518d\u5229\u7528\u3067\u304d\u307e\u3059\u3002<\/p>\n<h3>\u30b8\u30e3\u30ab\u30ea\u30f3\u3092\u7528\u3044\u305fIgA\u7cbe\u88fd<\/h3>\n<p>\u30b8\u30e3\u30ab\u30ea\u30f3\u306f\u3001\u30b8\u30e3\u30c3\u30af\u30d5\u30eb\u30fc\u30c4\u306e\u7a2e\u304b\u3089\u62bd\u51fa\u3055\u308c\u308b\u03b1-D-galactose\u7d50\u5408\u6027\u30ec\u30af\u30c1\u30f3\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u3059\u3002<strong>\u30b8\u30e3\u30ab\u30ea\u30f3\u306f\u3001\u8840\u6e05\u4e2d\u306e\u30d2\u30c8IgA1\u3084\u5206\u6ccc\u578b\u30d2\u30c8IgA1\u306b\u7279\u7570\u7684\u306b\u7d50\u5408\u3059\u308b\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u3044\u307e\u3059\u3002<\/strong>\u30b8\u30e3\u30ab\u30ea\u30f3\u3068IgA\u3068\u306e\u7d50\u5408\u306fPBS\uff08phosphate buffered saline\uff09\u306e\u69d8\u306a\u751f\u7406\u7684\u30d0\u30c3\u30d5\u30a1\u30fc\u6761\u4ef6\u4e0b\u3067\u8d77\u3053\u308a\u307e\u3059\u3002IgA\u306e\u6eb6\u51fa\u306b\u306f\u7af6\u5408\u30ea\u30ac\u30f3\u30c9\uff08\u4f8b: 0.1M melibiose\u30840.1M \u03b1-D-galactose\uff09\u3092\u542b\u3080PBS\u3092\u4f7f\u7528\u3057\u307e\u3059\u3002\u5f0a\u793e\u3067\u306f\u30016%\u30a2\u30ac\u30ed\u30fc\u30b9\u306b\u30b8\u30e3\u30ab\u30ea\u30f3\u3092\u56fa\u5b9a\u5316\u3057\u305f<a href=\"http:\/\/www.thermofisher.com\/order\/catalog\/product\/20395?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa30_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Immobilized Jacalin<\/a>\u3092\u3054\u7528\u610f\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<h2>\u6700\u5f8c\u306b<\/h2>\n<p>\u3044\u304b\u304c\u3067\u3057\u305f\u3067\u3057\u3087\u3046\u304b\uff1f<\/p>\n<p>\u4eca\u56de\u7d39\u4ecb\u3057\u307e\u3057\u305f\u6297\u4f53\u30af\u30e9\u30b9\u30ec\u30d9\u30eb\u306e\u7cbe\u88fd\u6cd5\u306f\u3001\u6700\u3082\u5e83\u304f\u5229\u7528\u3055\u308c\u3066\u3044\u308b\u6297\u4f53\u7cbe\u88fd\u6cd5\u3067\u3059\u3002Crude\u30ec\u30d9\u30eb\u306e\u7cbe\u88fd\u6cd5\u3068\u7d44\u5408\u305b\u308b\u3053\u3068\u3067\u3001\u3055\u3089\u306b\u7cbe\u88fd\u7d14\u5ea6\u3092\u4e0a\u3052\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002\u307e\u3068\u3081\u306e\u610f\u5473\u3092\u3053\u3081\u3066\u3001\u88682\u306b\u6297\u4f53\u30af\u30e9\u30b9\u30ec\u30d9\u30eb\u306e\u7cbe\u88fd\u6cd5\u3092\u63b2\u8f09\u3057\u3066\u304a\u304d\u307e\u3059\u3002<\/p>\n<table>\n<tbody>\n<tr>\n<td style=\"text-align: center\"><strong>\u7cbe\u88fd\u65b9\u6cd5\uff0f\u30bf\u30fc\u30b2\u30c3\u30c8\u30af\u30e9\u30b9<\/strong><\/td>\n<td style=\"text-align: center\"><strong>\u539f\u7406<\/strong><\/td>\n<td style=\"text-align: center\"><strong>\u5f0a\u793e\u88fd\u54c1\u4f8b<\/strong><\/td>\n<\/tr>\n<tr>\n<td>\u30cd\u30ac\u30c6\u30a3\u30d6\u30bb\u30ec\u30af\u30b7\u30e7\u30f3<\/td>\n<td>\u8840\u6e05\u3001\u8179\u6c34\u3001\u57f9\u990a\u4e0a\u6e05\u4e2d\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u7d50\u5408\u3059\u308bMelon Gel\u306b\u3088\u308b\u7cbe\u88fd<\/td>\n<td><a class=\"ext-link ext-icon-2\" title=\"\" href=\"http:\/\/www.thermofisher.com\/order\/catalog\/product\/45212?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa30_Social_LAB\" target=\"_blank\" rel=\"external nofollow noopener noreferrer\" data-wpel-target=\"_blank\">Melon\u2122 Gel IgG Purification Kit<\/a><\/td>\n<\/tr>\n<tr>\n<td rowspan=\"4\">IgG\u306e\u7cbe\u88fd<\/td>\n<td rowspan=\"4\">\u6297\u4f53\u7d50\u5408\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u5229\u7528\u3057\u305f\u7cbe\u88fd<\/td>\n<td><a class=\"ext-link ext-icon-2\" title=\"\" href=\"http:\/\/www.thermofisher.com\/order\/catalog\/product\/89978?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa30_Social_LAB\" target=\"_blank\" rel=\"external nofollow noopener noreferrer\" data-wpel-target=\"_blank\">NAb\u2122 Protein A Plus Spin Kit<\/a><\/td>\n<\/tr>\n<tr>\n<td><a class=\"ext-link ext-icon-2\" title=\"\" href=\"http:\/\/www.thermofisher.com\/order\/catalog\/product\/89949?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa30_Social_LAB\" target=\"_blank\" rel=\"external nofollow noopener noreferrer\" data-wpel-target=\"_blank\">NAb\u2122 Protein G Spin Kit<\/a><\/td>\n<\/tr>\n<tr>\n<td><a class=\"ext-link ext-icon-2\" title=\"\" href=\"http:\/\/www.thermofisher.com\/order\/catalog\/product\/89950?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa30_Social_LAB\" target=\"_blank\" rel=\"external nofollow noopener noreferrer\" data-wpel-target=\"_blank\">NAb\u2122 Protein A\/G Spin Kit<\/a><\/td>\n<\/tr>\n<tr>\n<td><a class=\"ext-link ext-icon-2\" title=\"\" href=\"http:\/\/www.thermofisher.com\/order\/catalog\/product\/89951?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa30_Social_LAB\" target=\"_blank\" rel=\"external nofollow noopener noreferrer\" data-wpel-target=\"_blank\">NAb\u2122 Protein L Spin Kit<\/a><\/td>\n<\/tr>\n<tr>\n<td>IgG\uff08IgY\uff09\u306e\u7cbe\u88fd<\/td>\n<td>Thiophilic\u5438\u7740\u3092\u5229\u7528\u3057\u305f\u7cbe\u88fd<\/td>\n<td><a class=\"ext-link ext-icon-2\" title=\"\" href=\"http:\/\/www.thermofisher.com\/order\/catalog\/product\/44916?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa30_Social_LAB\" target=\"_blank\" rel=\"external nofollow noopener noreferrer\" data-wpel-target=\"_blank\">Pierce\u2122 Thiophilic Adsorption Kit<\/a><\/td>\n<\/tr>\n<tr>\n<td>IgM\u306e\u7cbe\u88fd<\/td>\n<td>\u30de\u30f3\u30ca\u30f3\u7d50\u5408\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u5229\u7528\u3057\u305f\u7cbe\u88fd<\/td>\n<td><a class=\"ext-link ext-icon-2\" title=\"\" href=\"http:\/\/www.thermofisher.com\/order\/catalog\/product\/44897?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa30_Social_LAB\" target=\"_blank\" rel=\"external nofollow noopener noreferrer\" data-wpel-target=\"_blank\">Pierce\u2122 IgM Purification Kit<\/a><\/td>\n<\/tr>\n<tr>\n<td>IgA\u306e\u7cbe\u88fd<\/td>\n<td>\u30b8\u30e3\u30ab\u30ea\u30f3\u3092\u5229\u7528\u3057\u305f\u7cbe\u88fd<\/td>\n<td><a class=\"ext-link ext-icon-2\" title=\"\" href=\"http:\/\/www.thermofisher.com\/order\/catalog\/product\/20395?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa30_Social_LAB\" target=\"_blank\" rel=\"external nofollow noopener noreferrer\" data-wpel-target=\"_blank\">Pierce\u2122 Jacalin Agarose<\/a><\/td>\n<\/tr>\n<tr>\n<td>IgY\u306e\u7cbe\u88fd<\/td>\n<td>\u5375\u767d\u304b\u3089\u306e\u8131\u8102\u304a\u3088\u3073\u6c88\u6bbf\u306b\u3088\u308b\u7cbe\u88fd<\/td>\n<td><a class=\"ext-link ext-icon-2\" title=\"\" href=\"http:\/\/www.thermofisher.com\/order\/catalog\/product\/44918?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa30_Social_LAB\" target=\"_blank\" rel=\"external nofollow noopener noreferrer\" data-wpel-target=\"_blank\">Pierce\u2122 Chicken IgY Purification Kit<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>\u6b21\u56de\u306f\u3001\u76ee\u7684\u6297\u539f\u306b\u7279\u7570\u7684\u306a\u6297\u4f53\u7cbe\u88fd\u3092\u884c\u3046\u65b9\u6cd5\uff08Specific\u306a\u7cbe\u88fd\uff09\u3092\u7d39\u4ecb\u3057\u307e\u3059\uff01<\/p>\n<h2>\u3010\u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3011\u30bf\u30f3\u30d1\u30af\u8cea\u89e3\u6790\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u30cf\u30f3\u30c9\u30d6\u30c3\u30af<\/h2>\n<p>\u52b9\u7387\u7684\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u62bd\u51fa\u304b\u3089\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u89e3\u6790\u30c4\u30fc\u30eb\u307e\u3067\u3001\u5305\u62ec\u7684\u306b\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u3092\u7d39\u4ecb\u3057\u3066\u304a\u308a\u307e\u3059\u3002PDF\u30d5\u30a1\u30a4\u30eb\u306e\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3092\u3054\u5e0c\u671b\u306e\u65b9\u306f\u3001\u4e0b\u8a18\u30dc\u30bf\u30f3\u3088\u308a\u304a\u7533\u8fbc\u307f\u304f\u3060\u3055\u3044\u3002<\/p>\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/catalogs-protein-form.html?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa30_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9<\/a><\/p>\n<h2>\u5171\u514d\u75ab\u6c88\u964d\u6cd5\u95a2\u9023\u3001\u305d\u306e\u4ed6\u306e\u8a18\u4e8b\u306f\u3053\u3061\u3089<\/h2>\n<table style=\"border-color: lightgray;border-collapse: collapse;padding: 20px\" border=\"1\">\n<tbody>\n<tr>\n<td style=\"border-color: lightgray;padding: 20px\">\n<ul>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic8\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa30_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u7b2c8\u56de\u3011in vitro \u30bf\u30f3\u30d1\u30af\u8cea\u76f8\u4e92\u4f5c\u7528\u3092\u89e3\u6790\u3059\u308b\u65b9\u6cd5\u307e\u3068\u3081<br \/>\n<\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic9\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa30_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u7b2c9\u56de\u3011\u5171\u514d\u75ab\u6c88\u964d\u6cd5\u306e\u300c\u554f\u984c\u70b9\u300d\u3068\u300c\u6539\u5584\u7b56\u300d\u307e\u3068\u3081<br \/>\n<\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic13\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa30_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u7b2c13\u56de\u3011\u3055\u307e\u3056\u307e\u306a\u6297\u4f53\u7cbe\u88fd\u6cd5\u3092\u307e\u3068\u3081\u3066\u307f\u305f \u305d\u306e2<br \/>\n<\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic14\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa30_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u7b2c14\u56de\u3011\u307f\u3093\u306a\u304c\u77e5\u308a\u305f\u3044\u30d7\u30ed\u30c6\u30a4\u30f3A\/G\/L\u306e\u6027\u8cea<br \/>\n<\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic21\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa30_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u7b2c21\u56de\u3011\u5171\u514d\u75ab\u6c88\u964d\u5b9f\u9a13\u306e\u57fa\u672c\u7684\u306a\u5b9f\u9a13\u624b\u9806<br \/>\n<\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic22\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa30_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u7b2c22\u56de\u3011\u5171\u514d\u75ab\u6c88\u964d\u5b9f\u9a13\u306b\u304a\u3051\u308b\u6297\u4f53\u306e\u56fa\u5b9a\u5316<br \/>\n<\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic23\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa30_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u7b2c23\u56de\u3011\u5171\u514d\u75ab\u6c88\u964d\u5b9f\u9a13\u306b\u304a\u3051\u308b\u30d0\u30c3\u30d5\u30a1\u30fc\u306b\u3064\u3044\u3066 <\/a><\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u7528\u306b\u306f\u4f7f\u7528\u3044\u305f\u3060\u3051\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u6297\u4f53\u306e\u7cbe\u88fd\u65b9\u6cd5\u306b\u306f\u3001\u5927\u304d\u304f\u5206\u3051\u3066\u3001Crude\u30ec\u30d9\u30eb\u306e\u7cbe\u88fd\u3001\u6297\u4f53\u30af\u30e9\u30b9\u30ec\u30d9\u30eb\u306e\u7cbe\u88fd\u3001Specific\u306a\u7cbe\u88fd\u306e3\u3064\u304c\u3042\u308a\u307e\u3059\u3002\u4eca\u56de\u306f\u305d\u308c\u305e\u308c\u306e\u30ec\u30d9\u30eb\u3054\u3068\u306b\u7cbe\u88fd\u65b9\u6cd5\u3092\u6319\u3052\u3066\u305d\u306e\u6982\u8981\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\uff01<\/p>\n","protected":false},"author":988,"featured_media":2243,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[156],"tags":[237,32],"division":[],"class_list":{"0":"post-153","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-mol-bio","8":"tag-protein-experiments","9":"tag-antibody","10":"entry"},"_selected_authors":[],"_selected_reviewers":"","acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.8 (Yoast SEO v27.8) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>\u3055\u307e\u3056\u307e\u306a\u6297\u4f53\u7cbe\u88fd\u6cd5\u3092\u307e\u3068\u3081\u3066\u307f\u305f \u305d\u306e1\uff5c\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\uff01\u30bf\u30f3\u30d1\u30af\u8cea\u5b9f\u9a13\u3042\u308c\u3053\u308c \u7b2c12\u56de - Learning at the Bench<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic12\/\" \/>\n<meta property=\"og:locale\" content=\"ja_JP\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"\u3055\u307e\u3056\u307e\u306a\u6297\u4f53\u7cbe\u88fd\u6cd5\u3092\u307e\u3068\u3081\u3066\u307f\u305f 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