{"id":15330,"date":"2023-09-14T17:48:29","date_gmt":"2023-09-14T08:48:29","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/?p=15330"},"modified":"2024-09-18T14:07:50","modified_gmt":"2024-09-18T05:07:50","slug":"gene-editing_bid_ts_7","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/gene-editing_bid_ts_7\/","title":{"rendered":"\u305d\u3046\u3044\u3046\u3053\u3068\u3060\u3063\u305f\u306e\u304b\uff01\u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\uff08CRISPR\/Cas9\uff09 \uff5e\u7b2c7\u56de\u3000\u30ce\u30c3\u30af\u30a4\u30f3\uff5e"},"content":{"rendered":"<p><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/gene-editing_bid_ts_6\/?CID=bid_syn_gns_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_lgn_kt_s00_gene_editing_bid_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u524d\u56de<\/a>\u306b\u5f15\u304d\u7d9a\u304d\u3001\u30b2\u30ce\u30e0\u7de8\u96c6\u306e\u521d\u5fc3\u8005A\u3055\u3093\u306b\u3088\u308b\u300c\u521d\u3081\u3066\u306e\u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\u300d\u30b7\u30ea\u30fc\u30ba\u3001\u7b2c7\u56de\u3067\u3059\u3002<br \/>\n<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-14304\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/05\/29d3bc99193cb0b837fb8cc53f050ae5-2.png\" alt=\"\" width=\"524\" height=\"283\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/05\/29d3bc99193cb0b837fb8cc53f050ae5-2.png 524w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/05\/29d3bc99193cb0b837fb8cc53f050ae5-2-300x162.png 300w\" sizes=\"auto, (max-width: 524px) 100vw, 524px\" \/><br \/>\nA\u3055\u3093\u306f\u3053\u308c\u307e\u3067\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u3068\u306a\u308b\u907a\u4f1d\u5b50\u306e\u6a5f\u80fd\u3092\u6b20\u5931\u3055\u305b\u308b\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u6cd5\u306b\u3064\u3044\u3066\u5b66\u3093\u3067\u304d\u307e\u3057\u305f\u3002\u3057\u304b\u3057\u30b2\u30ce\u30e0\u7de8\u96c6\u306b\u306f\u3001\u76ee\u7684\u306e\u907a\u4f1d\u5b50\u914d\u5217\u3092\u633f\u5165\u3059\u308b\u30ce\u30c3\u30af\u30a4\u30f3\u6cd5\u3082\u3042\u308a\u307e\u3059\u3002<br \/>\n\u7b2c7\u56de\u3067\u306f\u3001\u3053\u306e\u30ce\u30c3\u30af\u30a4\u30f3\u6cd5\u306e\u5b9f\u9a13\u30c7\u30b6\u30a4\u30f3\u3084\u30dd\u30a4\u30f3\u30c8\u306b\u3064\u3044\u3066\u3054\u7d39\u4ecb\u3057\u307e\u3057\u3087\u3046\u3002<\/p>\n<h2>\u30b2\u30ce\u30e0\u7de8\u96c6\u306e\u30ce\u30c3\u30af\u30a4\u30f3\u3068\u306f\uff1f<\/h2>\n<p><span style=\"color: #3366ff\">A\u3055\u3093<\/span>\uff1a<br \/>\n\u4eca\u307e\u3067\u30b2\u30ce\u30e0\u7de8\u96c6\u306e\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u306b\u3064\u3044\u3066\u5b66\u3093\u3067\u304d\u307e\u3057\u305f\u304c\u3001\u4eca\u5f8c\u306e\u305f\u3081\u306b\u6559\u3048\u3066\u304f\u3060\u3055\u3044\u3002\u76ee\u7684\u90e8\u4f4d\u306b\u7279\u5b9a\u306e\u914d\u5217\u3092\u633f\u5165\u3059\u308b\u3068\u304d\u3001\u3069\u3046\u3044\u3046\u6d41\u308c\u3067\u5b9f\u9a13\u3092\u3059\u308b\u3093\u3067\u3057\u3087\u3046\u304b\u3002<\/p>\n<p><span style=\"color: #ff00ff\">\u5148\u8f29<\/span>\uff1a<br \/>\n\u30ce\u30c3\u30af\u30a4\u30f3\u306e\u3053\u3068\u3060\u306d\u3002\u57fa\u672c\u306e\u6d41\u308c\u306f\u540c\u3058\u3060\u3088\u3002\u30b2\u30ce\u30e0\u7de8\u96c6\u306e\u4e3b\u5f792\u4eba\u306e\u307b\u304b\u306b\u30c9\u30ca\u30fc\u6838\u9178\u304c\u5fc5\u8981\u306b\u306a\u308b\u3093\u3060\u3002<\/p>\n<p><span style=\"color: #3366ff\">A\u3055\u3093<\/span>\uff1a<br \/>\n\u30b2\u30ce\u30e0\u7de8\u96c6\u306e2\u4eba\u306e\u4e3b\u5f79\u3001<strong>Cas9\u3068\u30ac\u30a4\u30c9RNA<\/strong>\u3067\u3059\u3088\u306d\uff01\u3053\u308c\u306b\u52a0\u3048\u3066\u30c9\u30ca\u30fc\u6838\u9178\u304c\u5fc5\u8981\u2026\u3002\u30c9\u30ca\u30fc\u6838\u9178\u3063\u3066\u306a\u3093\u3067\u3057\u3087\u3046\u304b\u3002<\/p>\n<p><span style=\"color: #ff00ff\">\u5148\u8f29<\/span>\uff1a<br \/>\n\u30c9\u30ca\u30fc\u6838\u9178\u306f\u3001\u30b2\u30ce\u30e0\u7de8\u96c6\u3067\u633f\u5165\u3057\u305f\u3044\u914d\u5217\u3092\u542b\u3093\u3060\u6838\u9178\u3060\u3088\u3002\u30d9\u30af\u30bf\u30fc\u3084\u77ed\u3044\u4e00\u672c\u9396\u30aa\u30ea\u30b4\u304c\u3088\u304f\u4f7f\u7528\u3055\u308c\u308b\u306d\u3002<\/p>\n<p>\u633f\u5165\u3057\u305f\u3044\u914d\u5217\uff08SNP\u3084\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u907a\u4f1d\u5b50\u306a\u3069\uff09\u304c\u6c7a\u307e\u3063\u3066\u3044\u308b\u5834\u5408\u306f\u3001\u30ce\u30c3\u30af\u30a4\u30f3\u3067\u30b2\u30ce\u30e0\u7de8\u96c6\u3092\u5b9f\u65bd\u3057\u307e\u3059\u3002\u30ce\u30c3\u30af\u30a4\u30f3\u30b7\u30b9\u30c6\u30e0\u306f\u3001\u30e9\u30f3\u30c0\u30e0\u5909\u7570\uff08\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\uff09\u306e\u5b9f\u9a13\u3068\u7570\u306a\u308a\u3001\u633f\u5165\u3059\u308b\u914d\u5217\u3092\u542b\u3093\u3060\u30c9\u30ca\u30fc\u30d9\u30af\u30bf\u30fc\u3084\u4e00\u672c\u9396\u30aa\u30ea\u30b4\uff08single strand oligo\uff09\u306e\u6e96\u5099\u304c\u5fc5\u8981\u3067\u3059\u3002\u307e\u305a\u306f\u56f31\u3067\u30ce\u30c3\u30af\u30a4\u30f3\u306e\u4ed5\u7d44\u307f\u3092\u78ba\u8a8d\u3057\u3066\u304a\u304d\u307e\u3057\u3087\u3046\u3002<\/p>\n<div id=\"attachment_15345\" style=\"width: 733px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-15345\" class=\"size-full wp-image-15345\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-01.jpg\" alt=\"\u30ce\u30c3\u30af\u30a4\u30f3\u306e\u4ed5\u7d44\u307f\" width=\"723\" height=\"376\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-01.jpg 723w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-01-300x156.jpg 300w\" sizes=\"auto, (max-width: 723px) 100vw, 723px\" \/><p id=\"caption-attachment-15345\" class=\"wp-caption-text\">\u56f31\uff1a\u30ce\u30c3\u30af\u30a4\u30f3\u306e\u4ed5\u7d44\u307f<\/p><\/div>\n<p>\u30ce\u30c3\u30af\u30a4\u30f3\u30b7\u30b9\u30c6\u30e0\u3067\u306f\u3001\u307e\u305a\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u3068\u540c\u69d8\u306b\u3001\u30ac\u30a4\u30c9RNA\u3068Cas9\u306b\u3088\u3063\u3066\u30bf\u30fc\u30b2\u30c3\u30c8\u90e8\u4f4d\u306b\u4e8c\u672c\u9396DNA\u5207\u65ad\uff08Double Strand Break\uff1aDSB\uff09\u304c\u8d77\u3053\u308a\u307e\u3059\u3002\u3053\u306e\u3068\u304d\u3001\u30c9\u30ca\u30fc\u6838\u9178\uff08\u30c9\u30ca\u30fc\u30d9\u30af\u30bf\u30fc\u3084\u4e00\u672c\u9396\u30aa\u30ea\u30b4\u306a\u3069\uff09\u304c\u7d30\u80de\u5185\u306b\u5171\u5b58\u3059\u308b\u3068\u3001DSB\u5f8c\u306b\u7d30\u80de\u5185\u306e\u76f8\u540c\u7d44\u307f\u63db\u3048\uff08Homology directed repair\uff1aHDR\uff09\u304c\u8d77\u3053\u308a\u3001\u30c9\u30ca\u30fc\u914d\u5217\u307e\u305f\u306f\u4e00\u672c\u9396\u30aa\u30ea\u30b4\u306e\u914d\u5217\u304c\u30b2\u30ce\u30e0\u306b\u53d6\u308a\u8fbc\u307e\u308c\u3001\u30ce\u30c3\u30af\u30a4\u30f3\u304c\u8d77\u3053\u308b\u3068\u3044\u3046\u4ed5\u7d44\u307f\u3067\u3059\u3002<\/p>\n<p><span style=\"color: #3366ff\">A\u3055\u3093<\/span>\uff1a<br \/>\n\u30c9\u30ca\u30fc\u6838\u9178\u306f\u3069\u3093\u306a\u30c7\u30b6\u30a4\u30f3\u3067\u3082\u3044\u3044\u3093\u3067\u3059\u304b\uff1fSNP\u3092\u5165\u308c\u66ff\u3048\u305f\u3044\u3068\u304d\u306f1\u5869\u57fa\u3060\u3051\u5165\u308c\u308b\u2026\u3063\u3066\u3053\u3068\u306f\u306a\u3044\u3067\u3059\u3088\u306d\u3002<\/p>\n<p><span style=\"color: #ff00ff\">\u5148\u8f29<\/span>\uff1a<br \/>\n\u306a\u3044\u306d\u3002\u30c9\u30ca\u30fc\u6838\u9178\u306b\u306f<b>\u633f\u5165\u3057\u305f\u3044\u914d\u5217\u3068\u3001DSB\u3068\u96a3\u63a5\u3057\u305f\u914d\u5217\u3068\u76f8\u540c\u6027\u3092\u6301\u3064\u30a2\u30fc\u30e0\u914d\u5217<\/b>\u306e\u4e21\u65b9\u304c\u5fc5\u8981\u3060\u3088\u3002\u305d\u3057\u3066\u3001\u300c\u76f8\u540c\u7d44\u307f\u63db\u3048\u304c\u8d77\u3053\u308a\u3084\u3059\u3044\u30c7\u30b6\u30a4\u30f3\u300d\u3063\u3066\u3044\u3046\u306e\u304c\u3042\u308b\u3093\u3060\u3002<\/p>\n<p>\u4e00\u822c\u7684\u306b\u3088\u304f\u4f7f\u308f\u308c\u308b\u30c9\u30ca\u30fc\u6838\u9178\u3068\u3057\u3066\u3001\u30d9\u30af\u30bf\u30fc\u3068\u4e00\u672c\u9396\u30aa\u30ea\u30b4\u304c\u3042\u308a\u307e\u3059\u3002\u4f7f\u3044\u5206\u3051\u306f\u3001\u6570\u5869\u57fa\u3084\u6570\u5341\u5869\u57fa\u306e\u5909\u7570\u306a\u3069\u3001\u633f\u5165\u3057\u305f\u3044\u914d\u5217\u3084\u5165\u308c\u66ff\u3048\u305f\u3044\u914d\u5217\u304c\u77ed\u3044\u5834\u5408\u306f\u4e00\u672c\u9396\u30aa\u30ea\u30b4DNA\u3001\u305d\u308c\u4ee5\u4e0a\u306e\u9577\u3044\u914d\u5217\u306f\u30c9\u30ca\u30fc\u30d9\u30af\u30bf\u30fc\u3092\u7528\u3044\u308b\u3053\u3068\u304c\u591a\u3044\u3067\u3059\u3002<\/p>\n<p>\u3069\u3046\u3044\u3046\u30c7\u30b6\u30a4\u30f3\u304c\u76f8\u540c\u7d44\u307f\u63db\u3048\u3092\u8d77\u3053\u3057\u3084\u3059\u304f\u3059\u308b\u306e\u304b\u306a\u3069\u3001\u305d\u308c\u305e\u308c\u306e\u30dd\u30a4\u30f3\u30c8\u3092\u4e0b\u8a18\u306b\u307e\u3068\u3081\u307e\u3057\u305f\u3002<\/p>\n<p><b>\uff1c\u30c9\u30ca\u30fc\u30d9\u30af\u30bf\u30fc\u3001\u30c7\u30b6\u30a4\u30f3\u3068\u9078\u629e\u306e\u30dd\u30a4\u30f3\u30c8\uff1e<\/b><\/p>\n<ul>\n<li>\u9577\u3044\u914d\u5217\u306e\u5834\u5408\u306f\u30c9\u30ca\u30fc\u30d9\u30af\u30bf\u30fc\u304c\u63a8\u5968<\/li>\n<li>\u633f\u5165\u3057\u305f\u3044\u914d\u5217\u306e\u5de6\u53f3\u306b\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u5468\u8fba\u914d\u5217\u3068\u76f8\u540c\u7684\u306a\u30a2\u30fc\u30e0\u914d\u5217\uff08500 bp\u4ee5\u4e0a\uff09\u3092\u8a2d\u3051\u308b<\/li>\n<li>\u7d44\u307f\u63db\u3048\u7387\u306f\u76f8\u540c\u9818\u57df\u9577\u306b\u4f9d\u5b58\u3057\u3001\u4e00\u822c\u7684\u306b\u633f\u5165\u914d\u5217\u304c\u9577\u3044\u307b\u3069\u30a2\u30fc\u30e0\uff08\u76f8\u540c\u914d\u5217\uff09\u3082\u9577\u304f\u5fc5\u8981<\/li>\n<li>DSB\u304b\u3089\u5c11\u306a\u304f\u3068\u3082100 bp\u4ee5\u5185\u304b\u3089\u76f8\u540c\u9818\u57df\u3092\u53d6\u5f97<\/li>\n<\/ul>\n<div id=\"attachment_15346\" style=\"width: 802px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-15346\" class=\"size-full wp-image-15346\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-02.jpg\" alt=\"\u30c9\u30ca\u30fc\u30d9\u30af\u30bf\u30fc\u306e\u30c7\u30b6\u30a4\u30f3\" width=\"792\" height=\"199\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-02.jpg 792w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-02-300x75.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-02-768x193.jpg 768w\" sizes=\"auto, (max-width: 792px) 100vw, 792px\" \/><p id=\"caption-attachment-15346\" class=\"wp-caption-text\">\u56f32\uff1a\u30c9\u30ca\u30fc\u30d9\u30af\u30bf\u30fc\u306e\u30c7\u30b6\u30a4\u30f3<\/p><\/div>\n<p><b>\uff1c\u4e00\u672c\u9396\u30aa\u30ea\u30b4DNA\u3001\u30c7\u30b6\u30a4\u30f3\u306e\u30dd\u30a4\u30f3\u30c8\uff1e<\/b><\/p>\n<ul>\n<li>\u6570\u5341\u5869\u57fa\u306a\u3069\u77ed\u3044\u914d\u5217\u306e\u5834\u5408\u306b\u63a8\u5968<\/li>\n<li>\u633f\u5165\u3057\u305f\u3044\u914d\u5217\u306e\u5de6\u53f3\u306b\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u5468\u8fba\u914d\u5217\u3068\u76f8\u540c\u7684\u306a\u30a2\u30fc\u30e0\u914d\u5217\uff0840\uff5e90\u5869\u57fa\uff09\u3092\u8a2d\u3051\u308b<\/li>\n<li>\u30a2\u30fc\u30e0\u914d\u5217\u306f\u9577\u3044\u65b9\u304c\u6bd4\u8f03\u7684\u30ce\u30c3\u30af\u30a4\u30f3\u52b9\u7387\u304c\u826f\u3044<\/li>\n<li>\u30bb\u30f3\u30b9\u3001\u30a2\u30f3\u30c1\u30bb\u30f3\u30b9\u9396\u3069\u3061\u3089\u3067\u3082\u5bfe\u5fdc\u53ef\u80fd<\/li>\n<\/ul>\n<div id=\"attachment_15347\" style=\"width: 791px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-15347\" class=\"size-full wp-image-15347\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-03.jpg\" alt=\"\u30c9\u30ca\u30fc\u4e00\u672c\u9396\u30aa\u30ea\u30b4DNA\u306e\u30c7\u30b6\u30a4\u30f3\" width=\"781\" height=\"157\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-03.jpg 781w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-03-300x60.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-03-768x154.jpg 768w\" sizes=\"auto, (max-width: 781px) 100vw, 781px\" \/><p id=\"caption-attachment-15347\" class=\"wp-caption-text\">\u56f33\uff1a\u30c9\u30ca\u30fc\u4e00\u672c\u9396\u30aa\u30ea\u30b4DNA\u306e\u30c7\u30b6\u30a4\u30f3<\/p><\/div>\n<p><span style=\"color: #3366ff\">A\u3055\u3093<\/span>\uff1a<br \/>\n\u4e00\u672c\u9396\u30aa\u30ea\u30b4DNA\u306f\u5408\u6210\u3067\u624b\u306b\u5165\u308c\u3089\u308c\u307e\u3059\u3088\u306d\u3002\u7cbe\u88fd\u30b0\u30ec\u30fc\u30c9\u306f\u3069\u306e\u304f\u3089\u3044\u304c\u3044\u3044\u3093\u3067\u3059\u304b\uff1f\u3042\u3068\u3001\u30d9\u30af\u30bf\u30fc\u306f\u3069\u3046\u3084\u3063\u3066\u4f5c\u88fd\u3059\u308c\u3070\u3044\u3044\u3093\u3067\u3057\u3087\u3046\u3002<\/p>\n<p><span style=\"color: #ff00ff\">\u5148\u8f29<\/span>\uff1a<br \/>\n\u4e00\u672c\u9396\u30aa\u30ea\u30b4DNA\u306e\u7cbe\u88fd\u5ea6\u306fPAGE\u7cbe\u88fd\u304c\u63a8\u5968\u3060\u3088\u3002\u30d9\u30af\u30bf\u30fc\u306f\u3001\u3069\u3046\u3044\u3063\u305f\u65b9\u6cd5\u3067\u3082\u3044\u3044\u3051\u3069\u3001Invitrogen\u2122 <a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/A13288?CID=bid_syn_gns_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_lgn_kt_s00_gene_editing_bid_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">GeneArt\u2122 Seamless kit<\/a>\u306a\u3089\u30013\u65ad\u7247\u3092\u30d9\u30af\u30bf\u30fc\u306b\u5c0e\u5165\u3067\u304d\u308b\u3088\u3002<br \/>\n\u3042\u3068\u306f\u3001Invitrogen\u2122 TrueTag\u2122 Donor DNA Kit\u30b7\u30ea\u30fc\u30ba\u307f\u305f\u3044\u306a\u30ad\u30c3\u30c8\u3092\u4f7f\u3048\u3070\u3001<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/A42992?CID=bid_syn_gns_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_lgn_kt_s00_gene_editing_bid_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">GFP<\/a>\u3084<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/A42993?CID=bid_syn_gns_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_lgn_kt_s00_gene_editing_bid_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">RFP<\/a>\u3001<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/A53809?CID=bid_syn_gns_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_lgn_kt_s00_gene_editing_bid_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">HA<\/a>\u3001<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/A53811?CID=bid_syn_gns_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_lgn_kt_s00_gene_editing_bid_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">6\u00d7His-tag<\/a>\u306a\u3069\u304c\u633f\u5165\u3067\u304d\u308b\u30c9\u30ca\u30fc\u6838\u9178\u304c\u6570\u6642\u9593\u3067\u4f5c\u308c\u308b\u3088\u3002\u86cd\u5149\u8272\u7d20\u3084\u304a\u306a\u3058\u307f\u306e\u914d\u5217\u3092\u5165\u308c\u305f\u3044\u5834\u5408\u306f\u3001\u3053\u3046\u3044\u3046\u88fd\u54c1\u3092\u63a2\u3057\u3066\u307f\u308b\u306e\u3082\u624b\u3060\u306d\u3002<\/p>\n<h2>\u30ce\u30c3\u30af\u30a4\u30f3\u306e\u30ac\u30a4\u30c9RNA\u30c7\u30b6\u30a4\u30f3<\/h2>\n<p><span style=\"color: #3366ff\">A\u3055\u3093<\/span>\uff1a<br \/>\n\u3068\u3053\u308d\u3067\u30ce\u30c3\u30af\u30a4\u30f3\u306e\u30ac\u30a4\u30c9RNA\u3068Cas9\u306f\u3001\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u3068\u540c\u3058\u3082\u306e\u3067\u3088\u3044\u3093\u3067\u3057\u3087\u3046\u304b\u3002<\/p>\n<p><span style=\"color: #ff00ff\">\u5148\u8f29<\/span>\uff1a<br \/>\nCas9\u306f\u540c\u3058\u3067\u3044\u3044\u3051\u3069\u3001\u30ac\u30a4\u30c9RNA\u306f\u6ce8\u610f\u304c\u5fc5\u8981\u3060\u3088\u3002<br \/>\n\u30ce\u30c3\u30af\u30a4\u30f3\u306e\u5834\u5408\u306f\u3001\u4e8c\u672c\u9396\u306e\u5207\u65ad\uff08DSB\uff09\u304c\u8d77\u3053\u3063\u305f\u90e8\u4f4d\u304b\u3089\u30ce\u30c3\u30af\u30a4\u30f3\u90e8\u4f4d\u307e\u3067\u306e\u8ddd\u96e2\u304c10\u5869\u57fa\u4ee5\u5185\u306b\u306a\u308b\u3088\u3046\u306b\u30c7\u30b6\u30a4\u30f3\u3059\u308b\u3068\u3001\u76f8\u540c\u7d44\u307f\u63db\u3048\u306e\u52b9\u7387\u304c\u9ad8\u3044\u3053\u3068\u304c\u308f\u304b\u3063\u3066\u308b\u3093\u3060\u3002<\/p>\n<p><span style=\"color: #3366ff\">A\u3055\u3093<\/span>\uff1a<br \/>\n10\u5869\u57fa\u4ee5\u5185\u3067\u3059\u304b\uff1fPAM\u914d\u5217\u3082\u3042\u308b\u3057\u3001\u30c7\u30b6\u30a4\u30f3\u304c\u9650\u5b9a\u3055\u308c\u305d\u3046\u3067\u3059\u306d\u3002<\/p>\n<p><span style=\"color: #ff00ff\">\u5148\u8f29<\/span>\uff1a<br \/>\n\u305d\u3046\u3060\u306d\u3002\u3067\u3082\u3001\u30ce\u30c3\u30af\u30a4\u30f3\u7528\u306e\u30ac\u30a4\u30c9RNA\u3082\u30bd\u30d5\u30c8\u30a6\u30a8\u30a2\u3067\u30c7\u30b6\u30a4\u30f3\u3067\u304d\u308b\u304b\u3089\u3001\u4efb\u305b\u3061\u3083\u3048\u3070\u3044\u3044\u3088\u3002<\/p>\n<p>\u30ce\u30c3\u30af\u30a4\u30f3\u7528\u306e\u30ac\u30a4\u30c9RNA\u306f\u3001<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/gene-editing_bid_ts_2\/?CID=bid_syn_gns_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_lgn_kt_s00_gene_editing_bid_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u7b2c2\u56de<\/a>\u3067\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u7528\u306e\u30ac\u30a4\u30c9RNA\u30c7\u30b6\u30a4\u30f3\u30c4\u30fc\u30eb\u3068\u3057\u3066\u3054\u7d39\u4ecb\u3057\u305f\u300cTrueDesign Genome Editor\u300d\u3092\u7528\u3044\u3066\u30c7\u30b6\u30a4\u30f3\u53ef\u80fd\u3067\u3059\u3002<\/p>\n<p>\u307e\u305a\u306f\u7b2c2\u56de\u306b\u3066\u89e3\u8aac\u3057\u305f\u65b9\u6cd5\u306b\u5f93\u3063\u3066\u3001\u300cTrueDesign Genome Editor\u300d\u3092\u958b\u304d\u30011. Select a gene editing experiment\u3092\u30af\u30ea\u30c3\u30af\u3057\u307e\u3057\u3087\u3046\u3002\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u306e\u3068\u304d\u306f\u300cGene Knockout\u300d\u3092\u9078\u629e\u3057\u307e\u3057\u305f\u304c\u3001\u30ce\u30c3\u30af\u30a4\u30f3\u306e\u3068\u304d\u306f\u3001\u305d\u306e\u53f3\u6a2a\u306e5\u3064\u306e\u9078\u629e\u80a2\u306e\u4e2d\u304b\u3089\u9078\u3073\u307e\u3059\u3002<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-15348\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-04.jpg\" alt=\"\" width=\"1500\" height=\"447\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-04.jpg 1500w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-04-300x89.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-04-1024x305.jpg 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-04-768x229.jpg 768w\" sizes=\"auto, (max-width: 1500px) 100vw, 1500px\" \/><\/p>\n<p>\u305d\u308c\u305e\u308c\u306e\u9078\u629e\u80a2\u306e\u610f\u5473\u306f\u3001\u4ee5\u4e0b\u306e\u3068\u304a\u308a\u3067\u3059\u3002<\/p>\n<ul>\n<li>Gene Knockout\uff1a\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u5b9f\u9a13<\/li>\n<li>Gene Tagging\uff1a\u30ce\u30c3\u30af\u30a4\u30f3\u5b9f\u9a13\u306e\u3046\u3061\u3001GFP\u3084RFP\u306a\u3069\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u633f\u5165\u3059\u308b\u5834\u5408<\/li>\n<li>Insertion\uff1a\u30ce\u30c3\u30af\u30a4\u30f3\u5b9f\u9a13\u306e\u3046\u3061\u300130\u5869\u57fa\u307e\u3067\u306e\u7279\u5b9a\u914d\u5217\u3092\u5165\u308c\u308b\u5834\u5408<\/li>\n<li>Deletion\uff1a\u30ce\u30c3\u30af\u30a4\u30f3\u5b9f\u9a13\u306e\u3046\u3061\u300130\u5869\u57fa\u307e\u3067\u306e\u7279\u5b9a\u914d\u5217\u3092\u9664\u53bb\u3059\u308b\u5834\u5408<\/li>\n<li>SNP Edit\uff1a\u30ce\u30c3\u30af\u30a4\u30f3\u5b9f\u9a13\u306e\u3046\u3061\u3001SNP\u306e1\u5869\u57fa\u3092\u5909\u66f4\u3057\u305f\u3044\u5834\u5408<\/li>\n<li>Replacement\uff1a\u30ce\u30c3\u30af\u30a4\u30f3\u5b9f\u9a13\u306e\u3046\u3061\u300130\u5869\u57fa\u307e\u3067\u306e\u7279\u5b9a\u914d\u5217\u3092\u3001\u5225\u306e\u7279\u5b9a\u914d\u5217\u306b\u7f6e\u304d\u63db\u3048\u308b\u5834\u5408<\/li>\n<\/ul>\n<p>\u3053\u3053\u3067\u306f\u4e00\u4f8b\u3068\u3057\u3066\u3001\u300cSNP Edit\u300d\u3092\u9078\u3093\u3067\u7d9a\u3051\u307e\u3059\u3002<br \/>\n\u300c2. Select Species\u300d\u3067\u751f\u7269\u7a2e\u3092\u9078\u629e\u3057\u307e\u3059\u3002\u3053\u3053\u306b\u8868\u793a\u3055\u308c\u3066\u3044\u308b\u4ee5\u5916\u306e\u751f\u7269\u7a2e\u306f\u30c7\u30b6\u30a4\u30f3\u304c\u3067\u304d\u307e\u305b\u3093\u306e\u3067\u3054\u6ce8\u610f\u304f\u3060\u3055\u3044\u3002<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-15350\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-05.jpg\" alt=\"\" width=\"768\" height=\"205\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-05.jpg 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-05-300x80.jpg 300w\" sizes=\"auto, (max-width: 768px) 100vw, 768px\" \/><br \/>\n\u4e00\u4f8b\u3068\u3057\u3066\u300cHuman\u300d\u3092\u9078\u629e\u3057\u3001\u300cNext\u300d\u3092\u30af\u30ea\u30c3\u30af\u3057\u307e\u3059\u3002<\/p>\n<p>\u300c3. Select Input Type\u300d\u3067\u3001\u907a\u4f1d\u5b50\u306e\u691c\u7d22\u306e\u4ed5\u65b9\u3092\u9078\u3073\u307e\u3059\u3002\u907a\u4f1d\u5b50\u540d\u304b\u3089\u691c\u7d22\u3057\u305f\u3044\u3068\u304d\u306f\u300cGene Symbol\u300d\u3001\u914d\u5217\u306e\u3068\u304d\u306f\u300cDNA Sequence\u300d\u3001\u67d3\u8272\u4f53\u306e\u30ed\u30b1\u30fc\u30b7\u30e7\u30f3\u304b\u3089\u691c\u7d22\u3059\u308b\u3068\u304d\u306f\u300cChromosome Locus\u300d\u3092\u9078\u3093\u3067\u304f\u3060\u3055\u3044\u3002<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-15351\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-06.jpg\" alt=\"\" width=\"510\" height=\"220\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-06.jpg 510w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-06-300x129.jpg 300w\" sizes=\"auto, (max-width: 510px) 100vw, 510px\" \/><br \/>\n\u3053\u3053\u3067\u306f\u3001\u591a\u304f\u306e\u4eba\u304c\u9078\u3070\u308c\u308b\u3067\u3042\u308d\u3046\u300cGene Symbol\u300d\u3092\u9078\u629e\u3057\u307e\u3059\u3002<br \/>\nGene Symbol\u3068\u3057\u3066\u3001\u8a66\u3057\u306b\u300cKLF4\u300d\u3068\u5165\u308c\u3066\u307f\u308b\u3068\u3001\u9078\u629e\u80a2\u304c\u8868\u793a\u3055\u308c\u307e\u3057\u305f\u3002<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-15333\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-07.jpg\" alt=\"\" width=\"872\" height=\"217\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-07.jpg 872w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-07-300x75.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-07-768x191.jpg 768w\" sizes=\"auto, (max-width: 872px) 100vw, 872px\" \/><br \/>\n\u8fd1\u3044\u907a\u4f1d\u5b50\u540d\u304c\u3042\u308b\u5834\u5408\u3001\u3053\u306e\u3088\u3046\u306b\u9078\u629e\u80a2\u304c\u8868\u793a\u3055\u308c\u307e\u3059\u3002\u30ce\u30c3\u30af\u30a4\u30f3\u3057\u305f\u3044\u907a\u4f1d\u5b50\u3092\u9078\u3073\u3001\u300cSearch Gene\u300d\u3092\u30af\u30ea\u30c3\u30af\u3057\u307e\u3057\u3087\u3046\u3002\u3053\u3053\u3067\u306f\u4e0a\u306e\u9078\u629e\u80a2\u3092\u9078\u3073\u307e\u3059\u3002<\/p>\n<p>\u300c5. Select one of the following xx transcript(s)\u300d\u3067\u306f\u3001\u305d\u306e\u907a\u4f1d\u5b50\u306b\u30d0\u30ea\u30a2\u30f3\u30c8\u304c\u3042\u308b\u5834\u5408\u3001\u3044\u305a\u308c\u304b\u3092\u9078\u3070\u306a\u304f\u3066\u306f\u306a\u308a\u307e\u305b\u3093\u3002\u300cTranscript ID\u300d\u3092\u30af\u30ea\u30c3\u30af\u3059\u308b\u3068\u3001NCBI\u306e\u30da\u30fc\u30b8\u306b\u98db\u3093\u3067\u3001\u8a73\u7d30\u304c\u78ba\u8a8d\u3067\u304d\u307e\u3059\u3002<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-15334\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-08.jpg\" alt=\"\" width=\"823\" height=\"181\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-08.jpg 823w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-08-300x66.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-08-768x169.jpg 768w\" sizes=\"auto, (max-width: 823px) 100vw, 823px\" \/><br \/>\n\u4e00\u4f8b\u3068\u3057\u3066\u3001\u4e0a\u306e\u907a\u4f1d\u5b50\u3092\u9078\u3073\u307e\u3059\u3002Select\u306b\u30c1\u30a7\u30c3\u30af\u3092\u5165\u308c\u3001\u300cNext\u300d\u3092\u30af\u30ea\u30c3\u30af\u3057\u3066\u304f\u3060\u3055\u3044\u3002<br \/>\n\u4e0b\u56f3\u306e\u3088\u3046\u306a\u753b\u9762\u304c\u8868\u793a\u3055\u308c\u307e\u3059\u3002\u307e\u305a\u306f\u4e0a\u534a\u5206\u304b\u3089\u3054\u8aac\u660e\u3057\u307e\u3057\u3087\u3046\u3002<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-15335\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-09.jpg\" alt=\"\" width=\"791\" height=\"198\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-09.jpg 791w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-09-300x75.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-09-768x192.jpg 768w\" sizes=\"auto, (max-width: 791px) 100vw, 791px\" \/><br \/>\n\u4e00\u90e8\u62e1\u5927\u3059\u308b\u3068\u3001\u4e0b\u56f3\u306e\u3088\u3046\u306b\u306a\u308a\u307e\u3059\u3002<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-15336\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-10.jpg\" alt=\"\" width=\"787\" height=\"308\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-10.jpg 787w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-10-300x117.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-10-768x301.jpg 768w\" sizes=\"auto, (max-width: 787px) 100vw, 787px\" \/><br \/>\n\u6700\u4e0a\u6bb5\u306e\u56f3\u306f\u907a\u4f1d\u5b50\u30de\u30c3\u30d7\u3067\u3001Intron\u3001Exon\u3001UTR\u3001Flanking sequence\u306b\u5206\u304b\u308c\u3066\u3044\u307e\u3059\u3002\u305d\u308c\u305e\u308c\u306e\u8272\u5206\u3051\u306f\u4e0b\u8a18\u306e\u3068\u304a\u308a\u3067\u3059\u3002<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-15337\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-11.jpg\" alt=\"\" width=\"401\" height=\"25\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-11.jpg 401w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-11-300x19.jpg 300w\" sizes=\"auto, (max-width: 401px) 100vw, 401px\" \/><br \/>\n\u4e0a\u304b\u30892\u6bb5\u76ee\u306e\u56f3\u300cSequence Editor\u300d\u306f\u3001\u907a\u4f1d\u5b50\u30de\u30c3\u30d7\u306e\u9752\u3044\u56db\u89d2\u5185\u3092\u62e1\u5927\u3057\u305f\u56f3\u3067\u3001\u907a\u4f1d\u5b50\u914d\u5217\u3001\u30a2\u30df\u30ce\u9178\u914d\u5217\u304c\u66f8\u304b\u308c\u3066\u3044\u307e\u3059\u3002<br \/>\n\u3067\u306f\u3001\u3053\u306e\u907a\u4f1d\u5b50\u306b\u5bfe\u3057\u3066SNP Edit\u3067\u304d\u308b\u30ce\u30c3\u30af\u30a4\u30f3\u7528\u306e\u30ac\u30a4\u30c9RNA\u3092\u30c7\u30b6\u30a4\u30f3\u3057\u3066\u307f\u307e\u3057\u3087\u3046\u3002\u307e\u305a\u306f\u300cSequence Editor\u300d\u3067\u3001\u7f6e\u63db\u3057\u305f\u3044SNP\u3001\u3064\u307e\u308a1\u5869\u57fa\u3092\u30af\u30ea\u30c3\u30af\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u305d\u3046\u3059\u308b\u3068\u3001\u753b\u9762\u4e0b\u90e8\u306b\u3001\u3069\u306e\u5869\u57fa\u306b\u7f6e\u63db\u3059\u308b\u304b\u9078\u629e\u80a2\u304c\u8868\u793a\u3055\u308c\u307e\u3059\u3002<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-15338\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-12.jpg\" alt=\"\" width=\"1136\" height=\"484\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-12.jpg 1136w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-12-300x128.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-12-1024x436.jpg 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-12-768x327.jpg 768w\" sizes=\"auto, (max-width: 1136px) 100vw, 1136px\" \/><\/p>\n<p>1\u5869\u57fa\u9078\u3093\u3067\u300cOK\u300d\u3092\u30af\u30ea\u30c3\u30af\u3059\u308b\u3068\u3001\u4e0b\u56f3\u306e\u3088\u3046\u306b\u300cEdit List\u300d\u306b\u5185\u5bb9\u304c\u5165\u308a\u307e\u3059\u3002<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-15339\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-13.jpg\" alt=\"\" width=\"1580\" height=\"200\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-13.jpg 1580w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-13-300x38.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-13-1024x130.jpg 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-13-768x97.jpg 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-13-1536x194.jpg 1536w\" sizes=\"auto, (max-width: 1580px) 100vw, 1580px\" \/><br \/>\n\u300cNext\u300d\u3092\u30af\u30ea\u30c3\u30af\u3059\u308b\u3068\u3001\u30ac\u30a4\u30c9RNA\u306e\u30c7\u30b6\u30a4\u30f3\u304c\u59cb\u307e\u308a\u307e\u3059\u3002<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-15340\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-14.jpg\" alt=\"\" width=\"686\" height=\"184\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-14.jpg 686w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-14-300x80.jpg 300w\" sizes=\"auto, (max-width: 686px) 100vw, 686px\" \/><\/p>\n<p>3\u7a2e\u985e\u306e\u30ac\u30a4\u30c9RNA\u304c\u30c7\u30b6\u30a4\u30f3\u3055\u308c\u307e\u3057\u305f\u3002\u305d\u308c\u305e\u308c\u306e\u9805\u76ee\u306b\u3064\u3044\u3066\u8aac\u660e\u3057\u307e\u3059\u3002<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-15341\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-15.jpg\" alt=\"\" width=\"1630\" height=\"394\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-15.jpg 1630w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-15-300x73.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-15-1024x248.jpg 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-15-768x186.jpg 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-15-1536x371.jpg 1536w\" sizes=\"auto, (max-width: 1630px) 100vw, 1630px\" \/><\/p>\n<ul>\n<li>CRISPR Target Sequence\uff1a<br \/>\n\u30ac\u30a4\u30c9RNA\u914d\u5217\u3067\u3059\u3002\u30af\u30ea\u30c3\u30af\u3059\u308b\u3068\u3001\u30da\u30fc\u30b8\u4e0a\u306eSequence Editor\u5185\u3067\u8a72\u5f53\u914d\u5217\u304c\u30cf\u30a4\u30e9\u30a4\u30c8\u3055\u308c\u307e\u3059\u3002<\/li>\n<li>PAM Site\uff1a<br \/>\nSqCas9\u306ePAM\u914d\u5217\u3067\u3059\u3002<br \/>\n\u203b\u3053\u306e\u30bd\u30d5\u30c8\u30a6\u30a8\u30a2\u3067\u306fSqCas9\u306e\u914d\u5217\u3057\u304b\u30c7\u30b6\u30a4\u30f3\u304c\u3067\u304d\u307e\u305b\u3093\u306e\u3067\u3054\u6ce8\u610f\u304f\u3060\u3055\u3044\u3002<\/li>\n<li>Score\uff08\uff05\uff09\uff1a<br \/>\n\u30a2\u30eb\u30b4\u30ea\u30ba\u30e0\u306e\u5408\u81f4\u7387\u3092\u793a\u3059\u30b9\u30b3\u30a2\u3067\u3059\u3002\u5408\u81f4\u7387\u304c\u9ad8\u3044\u307b\u3069\u30b9\u30b3\u30a2\u304c\u9ad8\u304f\u306a\u308a\u307e\u3059\u304c\u3001\u30b9\u30b3\u30a2\u304c\u9ad8\u3044\u304b\u3089\u3068\u8a00\u3063\u3066\u6d3b\u6027\u304c\u9ad8\u3044\u3068\u306f\u9650\u308a\u307e\u305b\u3093\u3002\u76ee\u5b89\u306e\u3072\u3068\u3064\u3068\u304a\u8003\u3048\u304f\u3060\u3055\u3044\u3002<\/li>\n<li>Off-Targets\uff1a<br \/>\n\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u3092\u5f15\u304d\u8d77\u3053\u3059\u53ef\u80fd\u6027\u306e\u3042\u308b\u914d\u5217\u306e\u6570\u3067\u3059\u3002\u30af\u30ea\u30c3\u30af\u3059\u308b\u3068\u3001\u5404\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u914d\u5217\u304c\u78ba\u8a8d\u3067\u304d\u307e\u3059\u3002<\/li>\n<li>Edit site distance\uff1a<br \/>\n\u4e8c\u672c\u9396\u5207\u65ad\u304c\u8d77\u3053\u3063\u305f\u7b87\u6240\u304b\u3089SNP\u307e\u3067\u306e\u8ddd\u96e2\u3067\u3059\u300210\u5869\u57fa\u4ee5\u5185\u306b\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002<\/li>\n<li>Donor DNA\uff1a<br \/>\n\u30c9\u30ca\u30fcDNA\u306e\u914d\u5217\u3067\u3059\u3002\u5909\u7570\u3084\u30a2\u30fc\u30e0\u3068\u306a\u308b\u76f8\u540c\u914d\u5217\u3092\u542b\u3093\u3060\u72b6\u614b\u3067\u8868\u793a\u3055\u308c\u3066\u3044\u307e\u3059\u3002<\/li>\n<li style=\"list-style-type: none\"><\/li>\n<\/ul>\n<p>Target Sequence\u306e\u53f3\u6a2a\u306b\u3064\u3044\u3066\u3044\u308b\u7dd1\u8272\u306e\u30c1\u30a7\u30c3\u30af\u306f\u3001\u8868\u793a\u3055\u308c\u305f4\u7a2e\u985e\u306e\u4e2d\u3067\u63a8\u5968\u3055\u308c\u308b\u914d\u5217\u3067\u3059\u3002\u7dd1\u8272\u306e\u30c1\u30a7\u30c3\u30af\u304c\u3064\u3044\u3066\u3044\u308b\u914d\u5217\u306e\u4e2d\u306b\u512a\u52a3\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u3053\u306e\u4f8b\u3067\u306f3\u3064\u306e\u914d\u5217\u306b\u30c1\u30a7\u30c3\u30af\u304c\u3064\u3044\u3066\u3044\u307e\u3059\u304c\u30013\u3064\u306e\u914d\u5217\u304c\u540c\u3058\u304f\u3089\u3044\u63a8\u5968\u3055\u308c\u308b\u3053\u3068\u3092\u793a\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>\u30b2\u30ce\u30e0\u7de8\u96c6\u3067\u306f\u3001\u30ac\u30a4\u30c9RNA\u306f\u307e\u305a1\u7a2e\u985e\u3092\u8cfc\u5165\u3057\u3066\u8a66\u3059\u3053\u3068\u304c\u591a\u3044\u3067\u3059\u3002\u6ce8\u6587\u3057\u305f\u3044\u30ac\u30a4\u30c9RNA\u306e\u3044\u305a\u308c\u304b\u3072\u3068\u3064\u306b\u30c1\u30a7\u30c3\u30af\u3092\u5165\u308c\u3066\u3001Next\u3092\u30af\u30ea\u30c3\u30af\u3057\u3066\u304f\u3060\u3055\u3044\u3002<br \/>\n\u6ce8\u6587\u30da\u30fc\u30b8\u306b\u98db\u3073\u307e\u3059\u3002<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-15343\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-16.jpg\" alt=\"\" width=\"872\" height=\"406\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-16.jpg 872w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-16-300x140.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-16-768x358.jpg 768w\" sizes=\"auto, (max-width: 872px) 100vw, 872px\" \/><\/p>\n<p>\u3053\u306e\u30da\u30fc\u30b8\u306e\u9805\u76ee\u3067\u306f\u3001\u6ce8\u6587\u3059\u308b\u3082\u306e\u306b\u30c1\u30a7\u30c3\u30af\u3092\u5165\u308c\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u5404\u9805\u76ee\u306b\u3064\u3044\u3066\u8aac\u660e\u3057\u307e\u3057\u3087\u3046\u3002<\/p>\n<p>\uff1c\u5de6\u5217\uff1e<\/p>\n<ul>\n<li>CRISPR gRNA Format\uff1a\u30c1\u30a7\u30c3\u30af\u3092\u5165\u308c\u3066\u304f\u3060\u3055\u3044\u3002\u30c7\u30b6\u30a4\u30f3\u3057\u305f\u30ac\u30a4\u30c9RNA\u3092\u30aa\u30fc\u30c0\u30fc\u3067\u304d\u307e\u3059\u3002\u5bb9\u91cf\u306f1.5 nmol\u306e\u307f\u3067\u3059\u3002<\/li>\n<li>Cas9 Format\uff1a\u30c1\u30a7\u30c3\u30af\u3092\u5916\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u4ee3\u7406\u5e97\u304b\u3089\u3054\u6ce8\u6587\u3092\u304a\u9858\u3044\u3044\u305f\u3057\u307e\u3059\u3002<\/li>\n<li>Cas9 Reagent\uff1a\u30c1\u30a7\u30c3\u30af\u3092\u5916\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u4ee3\u7406\u5e97\u304b\u3089\u3054\u6ce8\u6587\u3092\u304a\u9858\u3044\u3044\u305f\u3057\u307e\u3059\u3002\/li&gt;<\/li>\n<li>Transfection Reagent\uff1a\u30c1\u30a7\u30c3\u30af\u3092\u5916\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u4ee3\u7406\u5e97\u304b\u3089\u3054\u6ce8\u6587\u3092\u304a\u9858\u3044\u3044\u305f\u3057\u307e\u3059\u3002<br \/>\n\u203b\u300cCas9 Format\u300d\u300cCas9 Reagent\u300d\u300cTransfection Reagent\u300d\u306f\u3001\u65e5\u672c\u3067\u306f\u3053\u3053\u304b\u3089\u6ce8\u6587\u304c\u3067\u304d\u307e\u305b\u3093\u3002\u3082\u3057\u30c1\u30a7\u30c3\u30af\u3092\u3057\u305f\u307e\u307eCart\u306b\u5165\u308c\u308b\u3068\u3001\u300c\u6ce8\u6587\u3067\u304d\u307e\u305b\u3093\u300d\u3068\u3044\u3046\u30a2\u30e9\u30fc\u30c8\u304c\u51fa\u307e\u3059\u306e\u3067\u3001\u3053\u3053\u3067\u5916\u3057\u3066\u304a\u3044\u3066\u304f\u3060\u3055\u3044\u3002<\/li>\n<\/ul>\n<p>\uff1c\u53f3\u5217\uff1e<\/p>\n<ul>\n<li>Primers\uff1a\u30c1\u30a7\u30c3\u30af\u3092\u5165\u308c\u308b\u3068\u3001\u30df\u30b9\u30de\u30c3\u30c1\u7279\u7570\u7684\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u5207\u65ad\u89e3\u6790\uff08<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/gene-editing_bid_ts_5\/?CID=bid_syn_gns_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_lgn_kt_s00_gene_editing_bid_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u7b2c5\u56de<\/a>\u53c2\u7167\uff09\u3092\u5b9f\u65bd\u3059\u308b\u3068\u304d\u306ePCR\u7528\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u6ce8\u6587\u3067\u304d\u307e\u3059\u3002\u5bb9\u91cf\u306f25 nmol\u306e\u307f\u3067\u3059\u3002<br \/>\n\u300cView\u300d\u3092\u30af\u30ea\u30c3\u30af\u3059\u308b\u3068\u3001\u30ac\u30a4\u30c9RNA\u306b\u5bfe\u3057\u3066\u30c7\u30b6\u30a4\u30f3\u3055\u308c\u305fForward Primer\u3068Reverse Primer\u306e\u914d\u5217\u304c\u78ba\u8a8d\u3067\u304d\u307e\u3059\u3002\u30c7\u30d5\u30a9\u30eb\u30c8\u3060\u30683\u3064\u3059\u3079\u3066\u306b\u30c1\u30a7\u30c3\u30af\u304c\u5165\u3063\u3066\u3044\u308b\u306e\u3067\u30011\u7a2e\u985e\u3067\u3088\u3044\u5834\u5408\u306f\u3001\u3069\u308c\u304b1\u3064\u3060\u3051\u30c1\u30a7\u30c3\u30af\u3092\u5165\u308c\u3066\u304a\u304d\u307e\u3057\u3087\u3046\u3002<br \/>\n\u203b\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u914d\u5217\u306b\u5bfe\u3059\u308bPrimer\u306f\u3053\u306e\u30b5\u30a4\u30c8\u3067\u306f\u6ce8\u6587\u3067\u304d\u307e\u305b\u3093\u3002<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-15344\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-17.jpg\" alt=\"\" width=\"775\" height=\"176\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-17.jpg 775w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-17-300x68.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-17-768x174.jpg 768w\" sizes=\"auto, (max-width: 775px) 100vw, 775px\" \/><\/li>\n<li>Other products You May Need\uff1a\u3059\u3079\u3066\u30c1\u30a7\u30c3\u30af\u3092\u5916\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u305d\u306e\u4ed6\u306e\u8a66\u85ac\u304c\u5fc5\u8981\u306a\u5834\u5408\u306f\u3001\u5225\u9014\u4ee3\u7406\u5e97\u304b\u3089\u3054\u6ce8\u6587\u3092\u304a\u9858\u3044\u3044\u305f\u3057\u307e\u3059\u3002<\/li>\n<li>Download Designs and Protocol\uff1a\u30af\u30ea\u30c3\u30af\u3057\u3001\u3054\u6ce8\u6587\u5185\u5bb9\u306e\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3068\u4fdd\u5b58\u3092\u304a\u3059\u3059\u3081\u3057\u307e\u3059\u3002\u30b5\u30a4\u30c8\u3067\u30c7\u30b6\u30a4\u30f3\u3057\u305f\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u914d\u5217\u306a\u3069\u3082\u4fdd\u5b58\u3057\u3066\u304a\u3051\u308b\u305f\u3081\u3067\u3059\u3002<\/li>\n<\/ul>\n<p>\u30c1\u30a7\u30c3\u30af\u3092\u5165\u308c\u7d42\u3048\u305f\u3089\u3001\u300cAdd to Cart\u300d\u3092\u30af\u30ea\u30c3\u30af\u3057\u3066\u30ab\u30fc\u30c8\u306b\u5165\u308c\u307e\u3057\u3087\u3046\u3002<\/p>\n<p>\u3053\u306e\u3088\u3046\u306b\u30c7\u30b6\u30a4\u30f3\u3055\u308c\u305fcrRNA\u3092\u7528\u3044\u3066\u30b2\u30ce\u30e0\u7de8\u96c6\u3092\u884c\u3046\u3053\u3068\u3067\u3001\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u52b9\u679c\u3092\u53ef\u80fd\u306a\u9650\u308a\u9632\u3050\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002\u3057\u304b\u3057\u3001\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u304c\u30bc\u30ed\u306b\u306a\u308b\u308f\u3051\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u305d\u306e\u305f\u3081\u3001\u30b2\u30ce\u30e0\u7de8\u96c6\u682a\u3092\u6a39\u7acb\u3057\u305f\u969b\u306f\u3001\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u306b\u3088\u308b\u5909\u7570\u3092\u78ba\u8a8d\u3057\u307e\u3057\u3087\u3046\u3002\u3053\u306e\u30c4\u30fc\u30eb\u306e\u300cOff-Targets\u300d\u3067\u8868\u793a\u3055\u308c\u305f\u914d\u5217\u4ed8\u8fd1\u304c\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u3092\u5f15\u304d\u8d77\u3053\u3059\u53ef\u80fd\u6027\u306e\u9ad8\u3044\u914d\u5217\u3067\u3059\u306e\u3067\u3001\u305d\u306e\u4ed8\u8fd1\u306b\u3064\u3044\u3066\u3001\u30b7\u30fc\u30b1\u30f3\u30b9\u306a\u3069\u3067\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u5909\u7570\u304c\u5c0e\u5165\u3055\u308c\u3066\u3044\u306a\u3044\u3053\u3068\u3092\u78ba\u8a8d\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<p><span style=\"color: #3366ff\">A\u3055\u3093<\/span>\uff1a<br \/>\n\u30c7\u30b6\u30a4\u30f3\u3057\u305f\u30ac\u30a4\u30c9RNA\u3068Cas9\u3068\u30c9\u30ca\u30fc\u6838\u9178\u3092\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u3057\u305f\u5f8c\u306e\u6d41\u308c\u306f\u3001\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u3068\u540c\u3058\u3067\u3059\u3088\u306d\uff1f\u3000\u30ce\u30c3\u30af\u30a4\u30f3\u3067\u4f55\u304b\u307b\u304b\u306b\u6c17\u3092\u4ed8\u3051\u308b\u30dd\u30a4\u30f3\u30c8\u306f\u3042\u308a\u307e\u3059\u304b\uff1f<\/p>\n<p><span style=\"color: #ff00ff\">\u5148\u8f29<\/span>\uff1a<br \/>\n\u305d\u3046\u3060\u306d\u3001\u3072\u3068\u3064\u306f\u3001\u6839\u6c17\u3088\u304f\u884c\u3046\u3053\u3068\u304b\u306a\u3002\u76f8\u540c\u914d\u5217\u306e\u52b9\u7387\u306f\u9ad8\u304f\u306a\u3044\u304b\u3089\u3001\u4f4e\u3044\u52b9\u7387\u3067\u3042\u308b\u3053\u3068\u3092\u8e0f\u307e\u3048\u3066\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u306e\u6570\u3092\u5897\u3084\u3059\u3068\u304b\u3001\u5de5\u592b\u3059\u308b\u3068\u3044\u3044\u3088\u3002<br \/>\n\u3075\u305f\u3064\u3081\u306f\u3001\u30ce\u30c3\u30af\u30a4\u30f3\u306b\u3053\u3060\u308f\u3089\u306a\u3044\u3053\u3068\u3002\u30ce\u30c3\u30af\u30a4\u30f3\u306f\u3001\u300c\u76ee\u7684\u306e\u5834\u6240\u300d\u306b\u300c\u633f\u5165\u3057\u305f\u3044\u907a\u4f1d\u5b50\u3084\u5869\u57fa\u304c\u3042\u308b\u300d\u3001\u3053\u306e2\u3064\u304c\u305d\u308d\u3063\u305f\u3068\u304d\u306b\u9078\u629e\u3059\u308b\u65b9\u6cd5\u3060\u304b\u3089\u3001\u305f\u3068\u3048\u3070\u633f\u5165\u3057\u305f\u3044\u907a\u4f1d\u5b50\u3084\u5869\u57fa\u304c\u3069\u3053\u306b\u5165\u3063\u3066\u3082\u3044\u3044\u5834\u5408\u306f\u5225\u306e\u65b9\u6cd5\u3082\u3042\u308b\u3088\u3002\u72b6\u6cc1\u306b\u5fdc\u3058\u3066\u5b9f\u9a13\u624b\u6cd5\u3092\u9078\u3076\u3053\u3068\u3082\u3001\u5b9f\u9a13\u6210\u529f\u306e\u30dd\u30a4\u30f3\u30c8\u306e\u3072\u3068\u3064\u3060\u306d\u3002<\/p>\n<h2>\u307e\u3068\u3081\uff1a\u30ce\u30c3\u30af\u30a4\u30f3\u7528\u306e\u30c9\u30ca\u30fc\u6838\u9178\u3068\u30ac\u30a4\u30c9RNA\u306e\u30dd\u30a4\u30f3\u30c8<\/h2>\n<ul>\n<li>\u30ce\u30c3\u30af\u30a4\u30f3\u306e\u3068\u304d\u306fCas9\u3001\u30ac\u30a4\u30c9RNA\u306e\u307b\u304b\u3001\u30c9\u30ca\u30fc\u6838\u9178\u304c\u5fc5\u8981<\/li>\n<li>\u30c9\u30ca\u30fc\u6838\u9178\u3067\u4e3b\u306b\u4f7f\u308f\u308c\u308b\u306e\u306f\u30d9\u30af\u30bf\u30fc\u3068\u4e00\u672c\u9396\u30aa\u30ea\u30b4DNA\u3002\u5165\u308c\u305f\u3044\u633f\u5165\u914d\u5217\u306e\u9577\u3055\u306b\u3088\u3063\u3066\u6c7a\u3081\u307e\u3057\u3087\u3046\u3002<\/li>\n<li>\u30ce\u30c3\u30af\u30a4\u30f3\u7528\u30ac\u30a4\u30c9RNA\u306f\u3001DSB\u90e8\u4f4d\u304b\u3089\u30ce\u30c3\u30af\u30a4\u30f3\u90e8\u4f4d\u307e\u3067\u306e\u8ddd\u96e2\u304c10\u5869\u57fa\u4ee5\u5185\u306b\u306a\u308b\u3088\u3046\u306b\u3059\u308b<\/li>\n<li>\u30ce\u30c3\u30af\u30a4\u30f3\u7528\u30ac\u30a4\u30c9RNA\u306f\u3001\u30c7\u30b6\u30a4\u30f3\u30c4\u30fc\u30eb\u3067\u30c7\u30b6\u30a4\u30f3\u304c\u53ef\u80fd<\/li>\n<\/ul>\n<p>\u3055\u3066\u3001\u8a087\u56de\u306b\u4e98\u3063\u3066\u9023\u8f09\u3057\u3066\u304d\u305f\u300c\u305d\u3046\u3044\u3046\u3053\u3068\u3060\u3063\u305f\u306e\u304b\uff01\u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\u300d\u30b7\u30ea\u30fc\u30ba\u306f\u672c\u30d6\u30ed\u30b0\u3067\u6700\u7d42\u56de\u3067\u3059\u3002\u8aad\u8005\u306e\u7686\u3055\u307e\u306e\u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\u304c\u3064\u3064\u304c\u306a\u304f\u9032\u307f\u307e\u3059\u3088\u3046\u306b\u3002\u3082\u3057\u3054\u5b9f\u9a13\u3067\u8ff7\u3046\u3053\u3068\u304c\u3042\u308c\u3070\u3001\u30b5\u30fc\u30e2\u30d5\u30a3\u30c3\u30b7\u30e3\u30fc\u30b5\u30a4\u30a8\u30f3\u30c6\u30a3\u30d5\u30a3\u30c3\u30af\u307e\u3067\u304a\u6c17\u8efd\u306b\u304a\u554f\u3044\u5408\u308f\u305b\u304f\u3060\u3055\u3044\u3002<\/p>\n<p>\uff1c\u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\u30b7\u30ea\u30fc\u30ba\uff1e<br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/gene-editing_bid_ts_1\/?CID=bid_syn_gns_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_lgn_kt_s00_gene_editing_bid_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u305d\u3046\u3044\u3046\u3053\u3068\u3060\u3063\u305f\u306e\u304b ! \u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\uff08CRISPR\/Cas9\uff09 \uff5e\u7b2c1\u56de\u3000CRISPR\/Cas9\u30b7\u30b9\u30c6\u30e0\u306e\u539f\u7406\uff5e<\/a><br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/gene-editing_bid_ts_2\/?CID=bid_syn_gns_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_lgn_kt_s00_gene_editing_bid_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u305d\u3046\u3044\u3046\u3053\u3068\u3060\u3063\u305f\u306e\u304b ! \u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\uff08CRISPR\/Cas9\uff09 \uff5e\u7b2c2\u56de\u3000\u30ac\u30a4\u30c9RNA\u306e\u30c7\u30b6\u30a4\u30f3\uff5e<\/a><br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/gene-editing_bid_ts_3\/?CID=bid_syn_gns_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_lgn_kt_s00_gene_editing_bid_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u305d\u3046\u3044\u3046\u3053\u3068\u3060\u3063\u305f\u306e\u304b ! \u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\uff08CRISPR\/Cas9\uff09 \uff5e\u7b2c3\u56de\u30003\u3064\u306eCRIPSR\/Cas9\u5b9f\u9a13\u7cfb\uff5e<\/a><br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/gene-editing_bid_ts_4\/?CID=bid_syn_gns_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_lgn_kt_s00_gene_editing_bid_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u305d\u3046\u3044\u3046\u3053\u3068\u3060\u3063\u305f\u306e\u304b ! \u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\uff08CRISPR\/Cas9\uff09 \uff5e\u7b2c4\u56de\u3000\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\uff5e<\/a><br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/gene-editing_bid_ts_5\/?CID=bid_syn_gns_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_lgn_kt_s00_gene_editing_bid_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u305d\u3046\u3044\u3046\u3053\u3068\u3060\u3063\u305f\u306e\u304b ! \u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\uff08CRISPR\/Cas9\uff09 \uff5e\u7b2c5\u56de\u3000\u5909\u7570\u306e\u78ba\u8a8d\u65b9\u6cd5\uff5e<\/a><br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/gene-editing_bid_ts_6\/?CID=bid_syn_gns_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_lgn_kt_s00_gene_editing_bid_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u305d\u3046\u3044\u3046\u3053\u3068\u3060\u3063\u305f\u306e\u304b ! \u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\uff08CRISPR\/Cas9\uff09 \uff5e\u7b2c6\u56de\u3000\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u52b9\u679c\uff5e<\/a><br \/>\n\u305d\u3046\u3044\u3046\u3053\u3068\u3060\u3063\u305f\u306e\u304b ! \u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\uff08CRISPR\/Cas9\uff09 \uff5e\u7b2c7\u56de\u3000\u30ce\u30c3\u30af\u30a4\u30f3\uff5e<\/p>\n<p>\uff1c\u30b2\u30ce\u30e0\u7de8\u96c6\u306e\u57fa\u790e\u7684\u306a\u5185\u5bb9\uff1e<br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/crispr-cas9_basic_bid_ts_1\/?CID=bid_syn_gns_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_lgn_kt_s00_gene_editing_bid_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30b2\u30ce\u30e0\u7de8\u96c6\u306e\u539f\u7406\u3068\u624b\u5f15\u304d<\/a><br \/>\n<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-15349\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-end.png\" alt=\"\" width=\"903\" height=\"212\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-end.png 903w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-end-300x70.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/gene-editing_bid_ts_7-end-768x180.png 768w\" sizes=\"auto, (max-width: 903px) 100vw, 903px\" \/><\/p>\n<h3>CRISPR\/Cas9\u30b2\u30ce\u30e0\u7de8\u96c6\u3092\u59cb\u3081\u308b\u65b9\u306b\u304a\u3059\u3059\u3081\uff01\u30c8\u30ec\u30fc\u30cb\u30f3\u30b0\u3068\u8a66\u85ac\u304c\u30bb\u30c3\u30c8\u306b\u306a\u3063\u305f\u30b9\u30bf\u30fc\u30bf\u30fc\u30d1\u30c3\u30af<\/h3>\n<p>\u6280\u8853\u9769\u65b0\u304c\u8457\u3057\u3044CRISPER\/Cas\u30b2\u30ce\u30e0\u7de8\u96c6\u5206\u91ce\u306b\u304a\u3044\u3066\u3001\u8a66\u85ac\u306e\u9078\u5b9a\u306b\u6238\u60d1\u3046\u65b9\u3082\u5c11\u306a\u304f\u3042\u308a\u307e\u305b\u3093\u3002\u307e\u305fgRNA\uff08\u30ac\u30a4\u30c9RNA\uff09\u306e\u30c7\u30b6\u30a4\u30f3\u65b9\u6cd5\u3084\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306e\u30ce\u30a6\u30cf\u30a6\u306f\u5b9f\u9a13\u7d50\u679c\u3092\u5de6\u53f3\u3059\u308b\u91cd\u8981\u306a\u30dd\u30a4\u30f3\u30c8\u3067\u3059\u3002<br \/>\n\u305d\u3053\u3067\u8b1b\u7fa9\u3001\u5b9f\u6280\u30c8\u30ec\u30fc\u30cb\u30f3\u30b0\u3001\u5fc5\u8981\u306a\u8a66\u85ac\u3092\u30bb\u30c3\u30c8\u306b\u3057\u305f\u3068\u3066\u3082\u304a\u5f97\u306a\u30d1\u30c3\u30b1\u30fc\u30b8\u3092\u6e96\u5099\u3057\u307e\u3057\u305f\u3002\u8a66\u85ac\u306e\u9078\u5b9a\u304b\u3089\u30c8\u30e9\u30d6\u30eb\u30b7\u30e5\u30fc\u30c8\u307e\u3067\u3001\u30a8\u30ad\u30b9\u30d1\u30fc\u30c8\u304c\u3042\u306a\u305f\u306e\u5b9f\u9a13\u7cfb\u306e\u7acb\u3061\u4e0a\u3052\u3092\u5168\u529b\u3067\u30b5\u30dd\u30fc\u30c8\u3057\u307e\u3059\u3002<\/p>\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/products-and-services\/services\/technical-services\/starter-pack\/genome-editing.html?CID=bid_syn_gns_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_lgn_kt_s00_gene_editing_bid_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u8a73\u7d30\u3092\u898b\u308b<\/a><\/p>\n<h3>\uff3b\u7121\u6599\u516c\u958b\u4e2d\uff3dCRISPR\u30b2\u30ce\u30e0\u7de8\u96c6\u30ea\u30bd\u30fc\u30b9\u30ac\u30a4\u30c9<\/h3>\n<p>\u30b2\u30ce\u30e0\u7de8\u96c6\u306e\u57fa\u790e\u306e\u5fa9\u7fd2\u3001\u3088\u308a\u6df1\u3044\u77e5\u8b58\u306e\u7372\u5f97\u3001\u307e\u305f\u306f\u6700\u65b0\u306e\u7814\u7a76\u30c4\u30fc\u30eb\u306e\u691c\u7d22\u304c\u3067\u304d\u307e\u3059\u3002\u30b2\u30ce\u30e0\u7de8\u96c6\u306e\u4e2d\u6838\u3068\u3057\u3066\u3001\u521d\u5fc3\u8005\u304b\u3089\u7d4c\u9a13\u8005\u307e\u3067\u3001\u8c4a\u5bcc\u3067\u4fe1\u983c\u3067\u304d\u308b\u6280\u8853\u7684\u306a\u5185\u5bb9\u3092\u63d0\u4f9b\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/genome-editing-resource-guide-form-download.html?CID=bid_syn_gns_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_lgn_kt_s00_gene_editing_bid_ts_7_Social_LAB\" target=\"_blank\" 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