{"id":15355,"date":"2023-09-19T15:14:04","date_gmt":"2023-09-19T06:14:04","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/?p=15355"},"modified":"2024-09-18T16:03:45","modified_gmt":"2024-09-18T07:03:45","slug":"ce_basic_dna_gsd_ts_3-2","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/ce_basic_dna_gsd_ts_3-2\/","title":{"rendered":"\u3044\u307e\u3055\u3089\u805e\u3051\u306a\u3044\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u306e\u3053\u3068\uff1a\u3000\u5149\u4fe1\u53f7\u304b\u3089\u5869\u57fa\u3078\u306e\u60c5\u5831\u5909\u63db"},"content":{"rendered":"<p>\u30ad\u30e3\u30d4\u30e9\u30ea\u30fc\u30b7\u30fc\u30b1\u30f3\u30b5\u3067\u3001\u30c7\u30fc\u30bf\u304c\u30a2\u30ca\u30ed\u30b0\u4fe1\u53f7\u304b\u3089\u30c7\u30b8\u30bf\u30eb\u4fe1\u53f7\u306b\u5909\u63db\u3055\u308c\u3001\u54c1\u8cea\u30b9\u30b3\u30a2\u3068\u3068\u3082\u306b\u5869\u57fa\u304c\u5272\u308a\u5f53\u3066\u3089\u308c\u308b\u307e\u3067\u306e\u30d7\u30ed\u30bb\u30b9\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n<h2>\u5149\u4fe1\u53f7\u304b\u3089\u5869\u57fa\u3078\u306e\u5909\u63db<\/h2>\n<p><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/ce_basic_dna_gsd_ts_2\/?CID=gsd_cap_sbu_r04_jp_cp1425_pjt7502_gsd00000_0so_blg_op_awa_og_s00_ce_basic_dna_gsd_ts_3_2_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30d6\u30ed\u30b0 \u201c\u3044\u307e\u3055\u3089\u805e\u3051\u306a\u3044\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u306e\u3053\u3068 \uff5e\u305d\u306e\uff11\uff1aDNA\u30b7\u30fc\u30b1\u30f3\u30b9\u306e\u30b4\u30fc\u30eb\u30c9\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\u306b\u306a\u308b\u307e\u3067\uff5e\u201d<\/a> \u3067\u306f\u3001\u653e\u5c04\u6027\u7269\u8cea\u3068 X 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aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-15358\" class=\"size-large wp-image-15358\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/3e64595579600aa8e8b345634e6c79c5-1024x357.jpg\" alt=\"\u56f31. \u30b5\u30a4\u30af\u30eb\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u306e\u6982\u5ff5\u56f3\" width=\"760\" height=\"265\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/3e64595579600aa8e8b345634e6c79c5-1024x357.jpg 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/3e64595579600aa8e8b345634e6c79c5-300x105.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/3e64595579600aa8e8b345634e6c79c5-768x268.jpg 768w, 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Collection\u30bd\u30d5\u30c8\u30a6\u30a8\u30a2\u306f\u3001\u30e6\u30fc\u30b6\u30fc\u304c\u6a5f\u5668\u3092\u64cd\u4f5c\u3059\u308b\u305f\u3081\u306e\u30bd\u30d5\u30c8\u30a6\u30a8\u30a2\u3067\u3001\u96fb\u5727\u3001\u6cf3\u52d5\u6642\u9593\u3001\u9069\u7528\u3059\u308b\u30d5\u30a3\u30eb\u30bf\u30fc\u3001\u30ab\u30e1\u30e9\u30b9\u30d4\u30fc\u30c9\u306a\u3069\u306e\u30d1\u30e9\u30e1\u30fc\u30bf\u30fc\u3092\u8abf\u6574\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u96fb\u6c17\u6cf3\u52d5\u3067\u306f\u3001\u96fb\u5727\u3068\u6642\u9593\u304c\u3001\u6cf3\u52d5\u901f\u5ea6\u3068\u30d4\u30fc\u30af\u5206\u96e2\u80fd\u306b\u5f71\u97ff\u3059\u308b\u4e3b\u8981\u306a\u30d1\u30e9\u30e1\u30fc\u30bf\u30fc\u3068\u306a\u308a\u307e\u3059\u3002<br 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aria-describedby=\"caption-attachment-15359\" class=\"wp-image-15359\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/bb563e374e2a169336ebc0009b063ac0-1024x706.jpg\" alt=\"\u56f32. 4\u7a2e\u306e\u86cd\u5149\u8272\u7d20\u306e\u86cd\u5149\u30b9\u30da\u30af\u30c8\u30eb\u6982\u5ff5\u56f3\" width=\"532\" height=\"367\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/bb563e374e2a169336ebc0009b063ac0-1024x706.jpg 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/bb563e374e2a169336ebc0009b063ac0-300x207.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/bb563e374e2a169336ebc0009b063ac0-768x529.jpg 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/bb563e374e2a169336ebc0009b063ac0-96x65.jpg 96w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/bb563e374e2a169336ebc0009b063ac0.jpg 1055w\" sizes=\"auto, (max-width: 532px) 100vw, 532px\" \/><p id=\"caption-attachment-15359\" class=\"wp-caption-text\">\u56f32. 4\u7a2e\u306e\u86cd\u5149\u8272\u7d20\u306e\u86cd\u5149\u30b9\u30da\u30af\u30c8\u30eb\u6982\u5ff5\u56f3<\/p><\/div>\n<h2>\u5869\u57fa\u306b\u5272\u308a\u5f53\u3066\u3089\u308c\u308b\u54c1\u8cea\u30b9\u30b3\u30a2<\/h2>\n<p>\u30ad\u30e3\u30d4\u30e9\u30ea\u30fc\u96fb\u6c17\u6cf3\u52d5\u306b\u3088\u308b\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u306e\u30a8\u30e9\u30fc\u30e2\u30c7\u30eb\u306f\u3001Phil Green\u306b\u3088\u3063\u3066\u958b\u767a\u3055\u308c\u305f\u3001Phred\u3068\u547c\u3070\u308c\u308b\u30d7\u30ed\u30b0\u30e9\u30e0\u306b\u3088\u308a\u5b9a\u91cf\u5316\u3055\u308c\u307e\u3059\u3002Phred\u54c1\u8cea\u30b9\u30b3\u30a2Q\u306f\u3001\u30a8\u30e9\u30fc\u78ba\u7387P\u306e\u95a2\u6570\u3068\u3057\u3066\u3001Q = -10 log<sub>10<\/sub>(P) \u3068\u5b9a\u7fa9\u3055\u308c\u307e\u3059\u3002\u54c1\u8cea\u30b9\u30b3\u30a220\u306f\u3001\u30a8\u30e9\u30fc\u78ba\u7387\u304c100 \u5206\u306e 1 \u3001\u7cbe\u5ea6\u306f99%\u3067\u3042\u308b\u3053\u3068\u3092\u793a\u3057\u307e\u3059\u3002\u54c1\u8cea\u30b9\u30b3\u30a2 30 \u306f\u3001\u30a8\u30e9\u30fc\u78ba\u73871000 \u5206\u306e 1\u3001\u7cbe\u5ea6\u304c99.9%\u3067\u3042\u308b\u3053\u3068\u3092\u793a\u3057\u307e\u3059\u3002<\/p>\n<div id=\"attachment_15360\" style=\"width: 542px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-15360\" class=\"wp-image-15360\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/15916439a5e45eeeb97bcab9cf9fa611.jpg\" alt=\"\u56f33. \u5272\u308a\u5f53\u3066\u3089\u308c\u305f\u5869\u57fa\u3068\u54c1\u8cea\u30b9\u30b3\u30a2Q\uff08Quality Value: QV\uff09\u306e\u4f8b Sequencing Analysis Software 8 user guide\u3088\u308a\u629c\u7c8b\" width=\"532\" height=\"318\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/15916439a5e45eeeb97bcab9cf9fa611.jpg 1018w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/15916439a5e45eeeb97bcab9cf9fa611-300x179.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/09\/15916439a5e45eeeb97bcab9cf9fa611-768x459.jpg 768w\" sizes=\"auto, (max-width: 532px) 100vw, 532px\" \/><p id=\"caption-attachment-15360\" class=\"wp-caption-text\">\u56f33. 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href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/A38938?SID=srch-hj-A38938?CID=gsd_cap_sbu_r04_jp_cp1425_pjt7502_gsd00000_0so_blg_op_awa_og_s00_ce_basic_dna_gsd_ts_3_2_Social_LAB\" target=\"_blank\" rel=\"noopener\">Applied Biosystems\u2122 Sequencing Analysis\u30bd\u30d5\u30c8\u30a6\u30a8\u30a2<\/a>\u3092\u4f7f\u7528\u3057\u3066\u95b2\u89a7\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u307e\u305f\u3001<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/A53552?SID=srch-srp-A53552?CID=gsd_cap_sbu_r04_jp_cp1425_pjt7502_gsd00000_0so_blg_op_awa_og_s00_ce_basic_dna_gsd_ts_3_2_Social_LAB\" target=\"_blank\" rel=\"noopener\">Applied Biosystems\u2122 Variant Reporter\u2122 \u30bd\u30d5\u30c8\u30a6\u30a8\u30a2<\/a>\u3067\u306f\u3001\u5869\u57fa\u914d\u5217\u306e\u8868\u793a\u3001\u30ea\u30d5\u30a1\u30ec\u30f3\u30b9\u914d\u5217\u3068\u306e\u6bd4\u8f03\u3001\u5909\u7570\u306e\u691c\u51fa\u304c\u3067\u304d\u307e\u3059\u3002<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/A53548?SID=srch-hj-A53548?CID=gsd_cap_sbu_r04_jp_cp1425_pjt7502_gsd00000_0so_blg_op_awa_og_s00_ce_basic_dna_gsd_ts_3_2_Social_LAB\" target=\"_blank\" rel=\"noopener\">Applied Biosystems\u2122 SeqScape\u2122 \u30bd\u30d5\u30c8\u30a6\u30a8\u30a2<\/a>\u3067\u306f\u3001\u3055\u3089\u306b\u3001\u8907\u6570\u306e\u30ea\u30d5\u30a1\u30ec\u30f3\u30b9\u914d\u5217\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u3068\u306e\u6bd4\u8f03\u306a\u3069\u3082\u53ef\u80fd\u3067\u3059\u3002<br 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rel=\"noopener\">\u3053\u3061\u3089<\/a>\u3092\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002<\/p>\n<h2>\u307e\u3068\u3081<\/h2>\n<p>\u4eca\u56de\u306f\u3001\u30ad\u30e3\u30d4\u30e9\u30ea\u30fc\u30b7\u30fc\u30b1\u30f3\u30b5\u3067\u86cd\u5149\u304c\u691c\u51fa\u3055\u308c\u3001\u5869\u57fa\u306e\u60c5\u5831\u306b\u5909\u63db\u3055\u308c\u308b\u307e\u3067\u306e\u30d7\u30ed\u30bb\u30b9\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3057\u305f\u3002\u30ad\u30e3\u30d4\u30e9\u30ea\u30fc\u96fb\u6c17\u6cf3\u52d5\u306b\u3088\u308b\u30b7\u30fc\u30b1\u30f3\u30b9\u89e3\u6790\u306b\u3064\u3044\u3066\u3001\u3088\u308a\u8a73\u3057\u304f\u77e5\u308a\u305f\u3044\u65b9\u306f\u3001\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u306e\u57fa\u672c\u539f\u5247\u3092\u5b66\u3079\u308b\u8cc7\u6599\u3092\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3057\u3066\u3044\u305f\u3060\u3051\u307e\u3059\u3002<\/p>\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/sanger-sequencing-guide-download.html?CID=gsd_cap_sbu_r04_jp_cp1425_pjt7502_gsd00000_0so_blg_op_awa_og_s00_ce_basic_dna_gsd_ts_3_2_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/p>\n<p>\u307e\u305f\u3001\u30bd\u30d5\u30c8\u30a6\u30a8\u30a2\u3092\u7528\u3044\u305f\u30c7\u30fc\u30bf\u89e3\u6790\u306e\u30cf\u30f3\u30ba\u30aa\u30f3\u30c8\u30ec\u30fc\u30cb\u30f3\u30b0\u3082\u3054\u7528\u610f\u3057\u3066\u304a\u308a\u307e\u3059\u306e\u3067\u3001\u305c\u3072\u3054\u6d3b\u7528\u304f\u3060\u3055\u3044\u3002<br \/>\n\u30cf\u30f3\u30ba\u30aa\u30f3\u30c8\u30ec\u30fc\u30cb\u30f3\u30b0\u30b3\u30fc\u30b9\uff1a<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/technical-resources\/trainings\/capillary-sequencer-3.html?CID=gsd_cap_sbu_r04_jp_cp1425_pjt7502_gsd00000_0so_blg_op_awa_og_s00_ce_basic_dna_gsd_ts_3_2_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30ad\u30e3\u30d4\u30e9\u30ea\u30fc\u30b7\u30fc\u30b1\u30f3\u30b5\uff083\uff09\u30c7\u30fc\u30bf\u89e3\u6790\u3000\uff5e\u5909\u7570\u89e3\u6790\u7de8\uff5e<\/a><\/p>\n<p>&nbsp;<\/p>\n<p>\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u76ee\u7684\u304a\u3088\u3073\u305d\u306e\u624b\u7d9a\u304d\u4e0a\u3067\u306e\u4f7f\u7528\u306f\u3067\u304d\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u30ad\u30e3\u30d4\u30e9\u30ea\u30fc\u30b7\u30fc\u30b1\u30f3\u30b5\u3067\u3001\u30c7\u30fc\u30bf\u304c\u30a2\u30ca\u30ed\u30b0\u4fe1\u53f7\u304b\u3089\u30c7\u30b8\u30bf\u30eb\u4fe1\u53f7\u306b\u5909\u63db\u3055\u308c\u3001\u54c1\u8cea\u30b9\u30b3\u30a2\u3068\u3068\u3082\u306b\u5869\u57fa\u304c\u5272\u308a\u5f53\u3066\u3089\u308c\u308b\u307e\u3067\u306e\u30d7\u30ed\u30bb\u30b9\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002 \u5149\u4fe1\u53f7\u304b\u3089\u5869\u57fa\u3078\u306e\u5909\u63db \u30d6\u30ed\u30b0 \u201c\u3044\u307e\u3055\u3089\u805e\u3051\u306a\u3044\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u306e\u3053\u3068 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