{"id":15524,"date":"2023-10-17T16:39:45","date_gmt":"2023-10-17T07:39:45","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/?p=15524"},"modified":"2023-10-17T16:44:56","modified_gmt":"2023-10-17T07:44:56","slug":"ce-human-identification-pcr-gsd-ts-1","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/ce-human-identification-pcr-gsd-ts-1\/","title":{"rendered":"\u30d2\u30c8\u500b\u4eba\u8b58\u5225\u306e\u305f\u3081\u306e\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9PCR\u306e\u30c7\u30b6\u30a4\u30f3"},"content":{"rendered":"

\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u7528\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u306f\u3001Applied Biosystems\u2122 Primer Designer\u2122 \u30c4\u30fc\u30eb<\/a>\u306e\u3088\u3046\u306a\u30aa\u30f3\u30e9\u30a4\u30f3\u30c4\u30fc\u30eb\u3092\u4f7f\u7528\u3059\u308b\u3053\u3068\u3067\u3001\u7c21\u5358\u306b\u30c7\u30b6\u30a4\u30f3\u3067\u304d\u307e\u3059\u3002\u3067\u306f\u3001\u30d2\u30c8\u500b\u4eba\u8b58\u5225\u306e\u3088\u3046\u306a\u3001\u7279\u6b8a\u306a\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u30c7\u30b6\u30a4\u30f3\u306f\u3069\u3046\u3067\u3057\u3087\u3046\u304b\uff1f
\n\u4eca\u56de\u306f\u3001Thermo Fisher Scientific\u306e\u30b7\u30cb\u30a2\u30b9\u30bf\u30c3\u30d5\u30b5\u30a4\u30a8\u30f3\u30c6\u30a3\u30b9\u30c8\u3067\u3042\u308bJulio Mulero\u3078\u306e2015\u5e74\u306e\u30a4\u30f3\u30bf\u30d3\u30e5\u30fc\u5185\u5bb9\u3092\u7d39\u4ecb\u3057\u307e\u3059\u3002Julio Mulero\u306e\u30b0\u30eb\u30fc\u30d7 \u306f\u300124\u306e\u907a\u4f1d\u5b50\u5ea7\u3092\u540c\u6642\u306b\u5897\u5e45\u3059\u308bApplied Biosystems\u2122 GlobalFiler\u2122 PCR Amplification Kit<\/a>\u306a\u3069\u3001\u30d2\u30c8\u500b\u4eba\u8b58\u5225\u7528\u306e\u88fd\u54c1\u3092\u958b\u767a\u3057\u307e\u3057\u305f\u3002<\/p>\n

\u6cd5\u533b\u5b66\u5e02\u5834\u5411\u3051\u306e\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u30ad\u30c3\u30c8\u306e\u958b\u767a\u8981\u4ef6\u3092\u6559\u3048\u3066\u304f\u3060\u3055\u3044\u3002<\/h2>\n

\u5f53\u793e\u306f27-plex\u307e\u3067\u306ePCR\u30d9\u30fc\u30b9\u306e\u30ad\u30c3\u30c8\u3092\u958b\u767a\u3057\u3066\u3044\u307e\u3059\u304c\u3001\u7279\u6b8a\u306a\u958b\u767a\u8981\u4ef6\u304c\u3042\u308a\u307e\u3059\u3002\u5f53\u793e\u306e\u30a2\u30f3\u30d7\u30ea\u30b3\u30f3\u306f\u3001\u9577\u3055\u304c70\uff5e400 bp \u306b\u306a\u308b\u3088\u3046\u306b\u30c7\u30b6\u30a4\u30f3\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u3053\u308c\u306f\u3001\u30ad\u30e3\u30d4\u30e9\u30ea\u30fc\u96fb\u6c17\u6cf3\u52d5\u30671 bp\u306e\u5206\u89e3\u80fd\u3092\u5b9f\u73fe\u3059\u308b\u305f\u3081\u306e\u6700\u9069\u306a\u9818\u57df\u3067\u3059\u3002\u307e\u305f\u3001\u89e3\u6790\u3067\u304d\u308b\u907a\u4f1d\u5b50\u5ea7\u306e\u6570\u3092\u6700\u5927\u5316\u3059\u308b\u305f\u3081\u306b\u3001Applied Biosystems\u2122 3500 \u30b8\u30a7\u30cd\u30c6\u30a3\u30c3\u30af\u30a2\u30ca\u30e9\u30a4\u30b6\u306e\u3088\u3046\u306a 6\u8272\u7d20\u306b\u5bfe\u5fdc\u3057\u305f\u6a5f\u5668\u3092\u4f7f\u7528\u3057\u307e\u3059\u3002\u79c1\u305f\u3061\u306e\u30c7\u30b6\u30a4\u30f3\u306e\u30bf\u30fc\u30b2\u30c3\u30c8\u3068\u306a\u308b\u500b\u3005\u306e\u907a\u4f1d\u5b50\u5ea7\u306f\u653f\u5e9c\u6a5f\u95a2\u306b\u3088\u3063\u3066\u6c7a\u5b9a\u3055\u308c\u3066\u304a\u308a\u3001\u3053\u308c\u3089\u306e\u907a\u4f1d\u5b50\u5ea7\u306f\u56fd\u306b\u3088\u3063\u3066\u7570\u306a\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u3042\u308b\u96c6\u56e3\u3067\u306f\u975e\u5e38\u306b\u6709\u610f\u306a\u907a\u4f1d\u5b50\u5ea7\u304c\u3001\u4ed6\u306e\u96c6\u56e3\u3067\u306f\u305d\u3046\u3067\u306f\u306a\u3044\u3053\u3068\u3082\u3042\u308a\u307e\u3059\u3002\u3053\u308c\u306b\u5bfe\u3057\u3001Applied Biosystems\u2122 GlobalFiler\u2122 PCR Amplification Kit<\/a>\u306f\u3001\u6700\u3082\u6709\u610f\u3067\u5e83\u304f\u4f7f\u7528\u3055\u308c\u3066\u3044\u308b\u907a\u4f1d\u5b50\u5ea7\u3092\u3001\u4e16\u754c\u4e2d\u3067\u4f7f\u7528\u3067\u304d\u308b 1\u3064\u306e\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u306b\u7d71\u5408\u3057\u305f\u3082\u306e\u306b\u306a\u308a\u307e\u3059\u3002<\/p>\n

GlobalFiler PCR Amplification Kit\u306f 24-plex\u306b\u306a\u308a\u307e\u3059\u304c\u300127-plex\u306e\u88fd\u54c1\u306f\u4f55\u3067\u3059\u304b?<\/h2>\n

Applied Biosystems\u2122 Yfiler\u2122 Plus PCR Amplification Kit<\/a>\u306f\u300127-plex\u306e\u88fd\u54c1\u3067\u3059\u3002Y\u67d3\u8272\u4f53\u306f\u3001\u6e1b\u6570\u5206\u88c2\u6642\u306b\u7d44\u307f\u63db\u3048\u3092\u53d7\u3051\u306a\u3044\u305f\u3081\u3001\u305d\u306e\u591a\u69d8\u6027\u306f\u7a81\u7136\u5909\u7570\u306b\u3088\u3063\u3066\u751f\u3058\u307e\u3059\u3002\u3053\u306e\u30ad\u30c3\u30c8\u306f\u3001\u5927\u91cf\u306e\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9DNA\uff08\u5973\u6027\uff09\u306e\u306a\u304b\u3067\u3001\u975e\u5e38\u306b\u5c11\u91cf\u306e Y DNA\uff08\u7537\u6027)\u306e\u8b58\u5225\u304c\u5fc5\u8981\u3068\u306a\u308b\u6027\u7684\u66b4\u884c\u4e8b\u4ef6\u306e\u305f\u3081\u306b\u958b\u767a\u3055\u308c\u307e\u3057\u305f\u3002\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9\u306b\u5bfe\u3059\u308bY DNA\u306e\u6bd4\u7387\u306f\u30011000\u5206\u306e1\u3068\u3044\u3046\u4f4e\u3044\u6bd4\u7387\u306b\u306a\u308b\u3053\u3068\u3082\u3042\u308a\u307e\u3059\u3002<\/p>\n

\"1000\u7537\u6027\uff0f\u5973\u6027\u6df7\u5408\u30b5\u30f3\u30d7\u30eb\u306e\u30c7\u30fc\u30bf\u4f8b\"

\u56f3. 1:1000\u7537\u6027\uff0f\u5973\u6027\u6df7\u5408\u30b5\u30f3\u30d7\u30eb\u306e\u30c7\u30fc\u30bf\u4f8b
Powerful discrimination plus optimal performance with the Yfiler Plus PCR Amplification Kit (thermofisher.com)<\/a>\u3088\u308a\u629c\u7c8b<\/p><\/div>\n

\u3055\u3089\u306b\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u540c\u58eb\u306e\u76f8\u4e92\u4f5c\u7528\u3084\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u5897\u5e45\u3092\u6700\u5c0f\u9650\u306b\u6291\u3048\u306a\u304f\u3066\u306f\u306a\u308a\u307e\u305b\u3093\u3002\u6cd5\u533b\u5b66\u5206\u91ce\u3067\u306f\u3001PCR \u963b\u5bb3\u5264\u306b\u5f37\u3044\u30de\u30b9\u30bf\u30fc\u30df\u30c3\u30af\u30b9\u304c\u6c42\u3081\u3089\u308c\u307e\u3059\u304c\u3001\u5b89\u5b9a\u6027\u3084\u30b9\u30bf\u30bf\u30fc\u203b<\/sup>\u5f62\u6210\u3068\u3044\u3063\u305f\u4ed6\u306e\u6027\u80fd\u3068\u306e\u30d0\u30e9\u30f3\u30b9\u3092\u3068\u308a\u306a\u304c\u3089\u89e3\u6c7a\u3059\u308b\u306e\u306f\u96e3\u3057\u3044\u554f\u984c\u3067\u3059\u3002
\n\u203bSTR\u306e\u3088\u3046\u306a\u7e70\u308a\u8fd4\u3057\u914d\u5217\u306b\u5bfe\u3057\u3001DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u304c\u4f38\u9577\u4e2d\u306b\u30b9\u30ea\u30c3\u30d7\u3059\u308b\u3053\u3068\u3067\u5f15\u304d\u8d77\u3053\u308bPCR\u5897\u5e45\u306e\u30a2\u30fc\u30c1\u30d5\u30a1\u30af\u30c8<\/p>\n

PCR\u30de\u30b9\u30bf\u30fc\u30df\u30c3\u30af\u30b9\u306e\u6700\u9069\u5316\u3082\u5fc5\u8981\u306a\u306e\u3067\u3057\u3087\u3046\u304b\uff1f<\/h2>\n

\u306f\u3044\u3001\u6700\u9069\u5316\u306f\u5fc5\u8981\u3067\u3059\u3002\u30d7\u30e9\u30a4\u30de\u30fc\u30c7\u30b6\u30a4\u30f3\u306e\u6307\u91dd\u3068\u3057\u3066\u3001\u30d0\u30a4\u30aa\u30a4\u30f3\u30d5\u30a9\u30de\u30c6\u30a3\u30af\u30b9\u3092\u6d3b\u7528\u3057\u3066\u3044\u307e\u3059\u304c\u3001\u30c7\u30b6\u30a4\u30f3\u3057\u305f\u3082\u306e\u304c\u6027\u80fd\u30d1\u30e9\u30e1\u30fc\u30bf\u30fc\u3092\u6e80\u305f\u3057\u3066\u3044\u308b\u304b\u306e\u78ba\u8a8d\u3067\u3001\u30e9\u30dc\u4f5c\u696d\u306b\u4ee3\u308f\u308b\u3082\u306e\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u5f53\u793e\u306f\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u30c7\u30b6\u30a4\u30f3\u3068 PCR\u30de\u30b9\u30bf\u30fc\u30df\u30c3\u30af\u30b9\u306e\u8abf\u5408\u306b 10 \u5e74\u4ee5\u4e0a\u306e\u7d4c\u9a13\u304c\u3042\u308a\u307e\u3059\u304c\u3001\u30b5\u30f3\u30d7\u30eb\u306e\u907a\u4f1d\u5b50\u578b\u306e\u89e3\u91c8\u306b\u5f71\u97ff\u3092\u4e0e\u3048\u308b\u30a2\u30fc\u30c1\u30d5\u30a1\u30af\u30c8\uff08\u30b9\u30bf\u30bf\u30fc\u306a\u3069\uff09\u3092\u6700\u5c0f\u9650\u306b\u6291\u3048\u306a\u304c\u3089\u3001\u304a\u5ba2\u3055\u307e\u304c\u6700\u3082\u91cd\u8996\u3059\u308b\u30d1\u30e9\u30e1\u30fc\u30bf\u30fc \uff08\u524d\u8ff0\u3057\u305fY-STR \u3078\u306e\u7279\u7570\u6027\u3084\u3001\u6570\u30d4\u30b3\u30b0\u30e9\u30e0\u91cf\u306eDNA\u306e\u611f\u5ea6\u306a\u3069\uff09\u3092\u6700\u9069\u5316\u3059\u308b\u305f\u3081\u306b\u3001\u4f55\u5ea6\u3082\u7e70\u308a\u8fd4\u3057\u691c\u8a3c\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n

\u30d7\u30e9\u30a4\u30de\u30fc\u30c7\u30b6\u30a4\u30f3\u306b\u306f\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0 PCR \u3082\u542b\u307e\u308c\u307e\u3059\u304b?<\/h2>\n

Applied Biosystems\u2122 Quantifiler\u2122 Trio DNA Quantification Kit<\/a>\u3068Quantifiler\u2122 HP DNA Quantification Kit<\/a>\u306f\u3001\u79c1\u305f\u3061\u306e\u30b0\u30eb\u30fc\u30d7\u304c\u6cd5\u533b\u5b66\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u7528\u306b\u958b\u767a\u3057\u305f\u88fd\u54c1\u3067\u3059\u3002\u3053\u308c\u3089\u306f\u3001\u6cd5\u533b\u5b66\u30b5\u30f3\u30d7\u30eb\u304b\u3089\u30d4\u30b3\u30b0\u30e9\u30e0\u91cf\u306eDNA\u3092\u6b63\u78ba\u306b\u5b9a\u91cf\u3067\u304d\u308b\u3088\u3046\u30c7\u30b6\u30a4\u30f3\u3055\u308c\u3066\u304a\u308a\u3001\u540c\u6642\u306b\u3001DNA\u306e\u5206\u89e3\u3084PCR\u963b\u5bb3\u306a\u3069\u3001DNA \u306e\u54c1\u8cea\u306b\u95a2\u3059\u308b\u8ffd\u52a0\u60c5\u5831\u3082\u63d0\u4f9b\u3057\u307e\u3059\u3002\u3053\u306e\u60c5\u5831\u306f\u3001\u5f8c\u306e\u5de5\u7a0b\u3067\u3001\u30b5\u30f3\u30d7\u30eb\u3092\u3069\u306e\u3088\u3046\u306b\u51e6\u7406\u3059\u308b\u304b\u306e\u6307\u91dd\u3068\u306a\u308b\u305f\u3081\u3001\u304a\u5ba2\u3055\u307e\u306b\u3068\u3063\u3066\u5927\u304d\u306a\u4fa1\u5024\u304c\u3042\u308a\u307e\u3059\u3002
\n\uff08\u30a4\u30f3\u30bf\u30d3\u30e5\u30fc\u7d42\u4e86\uff09<\/p>\n

\u4eca\u56de\u306f\u3001\u30d2\u30c8\u500b\u4eba\u8b58\u5225\u7528\u306e\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u30ad\u30c3\u30c8\u3092\u958b\u767a\u3057\u305f\u5f53\u793e\u30b5\u30a4\u30a8\u30f3\u30c6\u30a3\u30b9\u30c8\u3078\u306e\u30a4\u30f3\u30bf\u30d3\u30e5\u30fc\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3057\u305f\u3002<\/p>\n

 <\/p>\n

Quantifiler Kit\u3001GlobalFiler Kit\u3001YFiler Kit\u306f\u3001\u7814\u7a76\u7528\u3001\u6cd5\u533b\u5b66\u7528\u3001\u89aa\u5b50\u9451\u5b9a\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u306b\u306f\u4f7f\u7528\u3044\u305f\u3060\u3051\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

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