{"id":15573,"date":"2023-10-26T16:53:36","date_gmt":"2023-10-26T07:53:36","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/?p=15573"},"modified":"2025-10-03T15:05:43","modified_gmt":"2025-10-03T06:05:43","slug":"nanodrop-dojo-bid-ts-2","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/nanodrop-dojo-bid-ts-2\/","title":{"rendered":"\u9023\u8f09NanoDrop\u9053\u5834 \u7b2c2\u56de \u3044\u308d\u3044\u308d\u306a\u6838\u9178\u30b5\u30f3\u30d7\u30eb\u306e\u5438\u5149\u5ea6\u3092\u6e2c\u5b9a\u3057\u3088\u3046\uff01\uff5ePCR\u7523\u7269\uff5e"},"content":{"rendered":"\n<p>NanoDrop\u3092\u7528\u3044\u305f\u6838\u9178\u30b5\u30f3\u30d7\u30eb\u306e\u5438\u5149\u5ea6\u6e2c\u5b9a\u306b\u3088\u308a\u3001\u5f97\u3089\u308c\u308b\u5438\u5149\u5ea6\u60c5\u5831\u304b\u3089\u6838\u9178\u6fc3\u5ea6\u3084\u593e\u96d1\u7269\u306e\u6709\u7121\u3092\u78ba\u8a8d\u3067\u304d\u307e\u3059\u3002\u3057\u304b\u3057\u3069\u306e\u3088\u3046\u306a\u6838\u9178\u30b5\u30f3\u30d7\u30eb\u306a\u3089\u6e2c\u5b9a\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u306a\u306e\u3067\u3057\u3087\u3046\u304b\uff1f \u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u3067\u306f\u3055\u307e\u3056\u307e\u306a\u6838\u9178\u30b5\u30f3\u30d7\u30eb\u3092\u53d6\u308a\u6271\u3044\u307e\u3059\u3002\u4eca\u56de\u3001\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u306b\u304a\u3044\u3066\u3055\u307e\u3056\u307e\u306a\u7528\u9014\u3067\u7528\u3044\u3089\u308c\u308bPCR\u7523\u7269\u306b\u3064\u3044\u3066\u3001\u5438\u5149\u5ea6\u6e2c\u5b9a\u304c\u53ef\u80fd\u304b\u3092\u78ba\u8a8d\u3057\u307e\u3059\u3002<\/p>\n\n\n\n<div class=\"wp-block-group is-vertical is-layout-flex wp-container-core-group-is-layout-8cf370e7 wp-block-group-is-layout-flex\">\n<p>\u25bc\u3053\u3093\u306a\u65b9\u306b\u304a\u3059\u3059\u3081\u3067\u3059\uff01<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u5438\u5149\u5ea6\u6e2c\u5b9a\u3092\u5b9f\u65bd\u3055\u308c\u3066\u3044\u308b\u65b9<\/li>\n\n\n\n<li>\u8d85\u5fae\u91cf\u5206\u5149\u5149\u5ea6\u8a08\u3092\u3054\u5229\u7528\u30fb\u3054\u691c\u8a0e\u306e\u65b9<\/li>\n<\/ul>\n<\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-pcr\u7523\u7269\u306e\u5438\u5149\u5ea6\u6e2c\u5b9a\">PCR\u7523\u7269\u306e\u5438\u5149\u5ea6\u6e2c\u5b9a<\/h2>\n\n\n\n<p>\u4eca\u56de\u691c\u8a3c\u306b\u7528\u3044\u308bPCR\u7523\u7269\u306f\u3001Plasmid DNA\u3092\u92f3\u578b\u3068\u3057\u3066Plasmid DNA\u5185\u306e\u4e00\u90e8\u914d\u5217\u3092\u4ee5\u4e0b\u306e\u30d7\u30ed\u30c8\u30b3\u30eb\u306b\u5f93\u3063\u3066\u5897\u5e45\u3057\u307e\u3057\u305f\u3002<\/p>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><tbody><tr><td><strong>\u8a66\u85ac<\/strong><\/td><td><strong>\u6dfb\u52a0\u91cf\uff08\u03bcL\uff09<\/strong><\/td><\/tr><tr><td>Plasmid DNA 1 ng \/ \u03bcL<\/td><td>1<\/td><\/tr><tr><td>2 \u00d7 Platinum SuperFi PCR Master Mix<\/td><td>25<\/td><\/tr><tr><td>10 \u03bcM \u30d5\u30a9\u30ef\u30fc\u30c9\u30d7\u30e9\u30a4\u30de\u30fc<\/td><td>2.5<\/td><\/tr><tr><td>10 \u03bcM \u30ea\u30d0\u30fc\u30b9\u30d7\u30e9\u30a4\u30de\u30fc<\/td><td>2.5<\/td><\/tr><tr><td>Nuclease Free Water<\/td><td>10<\/td><\/tr><tr><td>Total \u53cd\u5fdc\u6db2<\/td><td>50<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<div class=\"wp-block-group is-vertical is-content-justification-center is-layout-flex wp-container-core-group-is-layout-4b2eccd6 wp-block-group-is-layout-flex\">\n<figure class=\"wp-block-image aligncenter is-resized size-full wp-image-15583\"><img loading=\"lazy\" decoding=\"async\" width=\"612\" height=\"363\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/nanodrop-dojo-bid-ts-2-01.png\" alt=\"PCR\u53cd\u5fdc\u6761\u4ef6\" class=\"wp-image-15583\" style=\"width:612px;height:auto\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/nanodrop-dojo-bid-ts-2-01.png 612w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/nanodrop-dojo-bid-ts-2-01-300x178.png 300w\" sizes=\"auto, (max-width: 612px) 100vw, 612px\" \/><\/figure>\n\n\n\n<p class=\"has-text-align-center\"><strong>\u56f31\uff1aPCR\u53cd\u5fdc\u6761\u4ef6<\/strong><\/p>\n<\/div>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-group is-vertical is-content-justification-center is-layout-flex wp-container-core-group-is-layout-4b2eccd6 wp-block-group-is-layout-flex\">\n<figure class=\"wp-block-image aligncenter size-full wp-image-15584\"><img loading=\"lazy\" decoding=\"async\" width=\"1244\" height=\"436\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/nanodrop-dojo-bid-ts-2-02.png\" alt=\"PCR\u7523\u7269\u306e\u5438\u5149\u5ea6\u30b9\u30da\u30af\u30c8\u30eb\" class=\"wp-image-15584\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/nanodrop-dojo-bid-ts-2-02.png 1244w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/nanodrop-dojo-bid-ts-2-02-300x105.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/nanodrop-dojo-bid-ts-2-02-1024x359.png 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/nanodrop-dojo-bid-ts-2-02-768x269.png 768w\" sizes=\"auto, (max-width: 1244px) 100vw, 1244px\" \/><\/figure>\n\n\n\n<p class=\"has-text-align-center\"><strong>\u56f32\uff1aPCR\u7523\u7269\u306e\u5438\u5149\u5ea6\u30b9\u30da\u30af\u30c8\u30eb<\/strong><\/p>\n<\/div>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>\u5f97\u3089\u308c\u305fPCR\u7523\u7269\u306e\u5438\u5149\u5ea6\u3092\u6e2c\u5b9a\u3057\u305f\u3068\u3053\u308d\u3001\u5438\u5149\u5ea6\u306e\u30b9\u30da\u30af\u30c8\u30eb\u60c5\u5831\u306f\u6838\u9178\u30b5\u30f3\u30d7\u30eb\u3092\u6e2c\u5b9a\u3057\u305f\u5438\u5149\u30b9\u30da\u30af\u30c8\u30eb\uff08260 nm\u306b\u6975\u5927\u306e\u5438\u53ce\u3092\u6709\u3059\u308b\uff09\u3068\u9177\u4f3c\u3057\u305f\u7d50\u679c\u3068\u306a\u308a\u307e\u3059\u3002\u3064\u307e\u308aPCR\u7523\u7269\u306e\u5438\u5149\u5ea6\u6e2c\u5b9a\u304c\u3067\u304d\u3066\u3044\u308b\u3068\u3044\u3046\u3053\u3068\u306b\u306a\u308b\u306e\u3067\u3057\u3087\u3046\u304b\uff1f<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-pcr\u53cd\u5fdc\u6db2\u306b\u542b\u307e\u308c\u308b\u3055\u307e\u3056\u307e\u306a\u593e\u96d1\u7269\">PCR\u53cd\u5fdc\u6db2\u306b\u542b\u307e\u308c\u308b\u3055\u307e\u3056\u307e\u306a\u593e\u96d1\u7269<\/h2>\n\n\n\n<p>PCR\u7523\u7269\u306e\u5438\u5149\u5ea6\u6e2c\u5b9a\u304b\u3089\u5f97\u3089\u308c\u308b\u30b9\u30da\u30af\u30c8\u30eb\u306f\u3001\u4e00\u898bPCR\u3067\u5897\u5e45\u3055\u308c\u305f\u6838\u9178\u304c\u6e2c\u5b9a\u3055\u308c\u3066\u3044\u308b\u3088\u3046\u306b\u78ba\u8a8d\u3055\u308c\u307e\u3059\u3002\u3053\u306e\u60c5\u5831\u3092\u3082\u3068\u306b\u5b9f\u9a13\u3092\u884c\u3063\u3066\u3082\u554f\u984c\u306a\u3044\u3067\u3057\u3087\u3046\u304b\uff1f<\/p>\n\n\n\n<p>\u7d50\u8ad6\u304b\u3089\u7533\u3057\u307e\u3059\u3068PCR\u7523\u7269\u306e\u5438\u5149\u5ea6\u6e2c\u5b9a\u306f\u6b63\u3057\u304fPCR\u7523\u7269\u3092\u6e2c\u5b9a\u3067\u304d\u3066\u3044\u308b\u3068\u306f\u9650\u308a\u307e\u305b\u3093\u3002\u7406\u7531\u3068\u3057\u307e\u3057\u3066\u3001PCR\u53cd\u5fdc\u6db2\u306f\u92f3\u578b\u306b\u7528\u3044\u305fPlasmid DNA\u4ee5\u5916\u306b\u3082\u3055\u307e\u3056\u307e\u306a\u201c\u6838\u9178\u201c\u304c\u542b\u307e\u308c\u3066\u3044\u307e\u3059\u3002\u305d\u308c\u3092\u793a\u3059\u305f\u3081\u306b\u3001Plasmid DNA\u3092\u6dfb\u52a0\u305b\u305a\u306bPCR\u53cd\u5fdc\u3092\u5b9f\u65bd\u3057\u305fPlasmid DNA free\u306ePCR\u7523\u7269\u306e\u5438\u5149\u5ea6\u6e2c\u5b9a\u3092\u884c\u3044\u307e\u3057\u305f\uff08\u56f33\uff09\u3002<\/p>\n\n\n\n<div class=\"wp-block-group is-vertical is-content-justification-center is-layout-flex wp-container-core-group-is-layout-4b2eccd6 wp-block-group-is-layout-flex\">\n<figure class=\"wp-block-image aligncenter size-full wp-image-15576\"><img loading=\"lazy\" decoding=\"async\" width=\"1233\" height=\"427\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/nanodrop-dojo-bid-ts-2-03.png\" alt=\"Plasmid\u6dfb\u52a0\u306e\u306a\u3044PCR\u7523\u7269\u306e\u5438\u5149\u5ea6\u30b9\u30da\u30af\u30c8\u30eb\" class=\"wp-image-15576\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/nanodrop-dojo-bid-ts-2-03.png 1233w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/nanodrop-dojo-bid-ts-2-03-300x104.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/nanodrop-dojo-bid-ts-2-03-1024x355.png 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/nanodrop-dojo-bid-ts-2-03-768x266.png 768w\" sizes=\"auto, (max-width: 1233px) 100vw, 1233px\" \/><\/figure>\n\n\n\n<p class=\"has-text-align-center\"><strong>\u56f33\uff1aPlasmid\u6dfb\u52a0\u306e\u306a\u3044PCR\u7523\u7269\u306e\u5438\u5149\u5ea6\u30b9\u30da\u30af\u30c8\u30eb<\/strong><\/p>\n<\/div>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>\u3044\u304b\u304c\u3067\u3057\u3087\u3046\u304b\uff1fPlasmid DNA\u3092\u92f3\u578b\u3068\u3057\u306a\u3044PCR\u7523\u7269\u3067\u3082\u3001\u6838\u9178\u30b5\u30f3\u30d7\u30eb\u3092\u6e2c\u5b9a\u3057\u305f\u5834\u5408\u3068\u9177\u4f3c\u3059\u308b\u5438\u5149\u30b9\u30da\u30af\u30c8\u30eb\u3092\u793a\u3057\u307e\u3059\u3002\u3053\u306e\u8981\u56e0\u306fPCR\u53cd\u5fdc\u6db2\u306b\u542b\u307e\u308c\u308b\u300c\u30d7\u30e9\u30a4\u30de\u30fc\u300d\u3068\u300cdNTP mix\u300d\u306b\u3088\u308b\u3082\u306e\u3067\u3059\u3002<\/p>\n\n\n\n<p>\u3064\u307e\u308a\u3001PCR\u53cd\u5fdc\u306b\u7528\u3044\u308b\u30d7\u30e9\u30a4\u30de\u30fc\u3082dNTP mix\u3082\u300c\u6838\u9178\u300d\u3067\u3042\u308b\u305f\u3081260 nm\u306b\u5438\u5149\u5ea6\u3092\u6709\u3057\u307e\u3059\u3002\u4ee5\u4e0b\u306f\u30d7\u30e9\u30a4\u30de\u30fc\u3068dNTP mix\u306e\u5438\u5149\u5ea6\u30b9\u30da\u30af\u30c8\u30eb\u306b\u306a\u308a\u307e\u3059\u3002<\/p>\n\n\n\n<div class=\"wp-block-group is-vertical is-content-justification-center is-layout-flex wp-container-core-group-is-layout-4b2eccd6 wp-block-group-is-layout-flex\">\n<figure class=\"wp-block-image aligncenter is-resized size-full wp-image-15577\"><img loading=\"lazy\" decoding=\"async\" width=\"1272\" height=\"464\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/nanodrop-dojo-bid-ts-2-04.png\" alt=\"\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u5438\u5149\u5ea6\u30b9\u30da\u30af\u30c8\u30eb\" class=\"wp-image-15577\" style=\"width:822px\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/nanodrop-dojo-bid-ts-2-04.png 1272w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/nanodrop-dojo-bid-ts-2-04-300x109.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/nanodrop-dojo-bid-ts-2-04-1024x374.png 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/nanodrop-dojo-bid-ts-2-04-768x280.png 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/nanodrop-dojo-bid-ts-2-04-960x350.png 960w\" sizes=\"auto, (max-width: 1272px) 100vw, 1272px\" \/><\/figure>\n\n\n\n<p class=\"has-text-align-center\"><strong>\u56f34\uff1a\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u5438\u5149\u5ea6\u30b9\u30da\u30af\u30c8\u30eb<\/strong><\/p>\n<\/div>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-group is-vertical is-content-justification-center is-layout-flex wp-container-core-group-is-layout-4b2eccd6 wp-block-group-is-layout-flex\">\n<figure class=\"wp-block-image aligncenter is-resized size-full wp-image-15578\"><img loading=\"lazy\" decoding=\"async\" width=\"1272\" height=\"478\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/nanodrop-dojo-bid-ts-2-05.png\" alt=\"dNTP mix\u306e\u5438\u5149\u5ea6\u30b9\u30da\u30af\u30c8\u30eb\" class=\"wp-image-15578\" style=\"width:822px;height:auto\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/nanodrop-dojo-bid-ts-2-05.png 1272w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/nanodrop-dojo-bid-ts-2-05-300x113.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/nanodrop-dojo-bid-ts-2-05-1024x385.png 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/nanodrop-dojo-bid-ts-2-05-768x289.png 768w\" sizes=\"auto, (max-width: 1272px) 100vw, 1272px\" \/><\/figure>\n\n\n\n<p><strong>\u56f35\uff1adNTP mix\u306e\u5438\u5149\u5ea6\u30b9\u30da\u30af\u30c8\u30eb<\/strong><\/p>\n<\/div>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>PCR\u53cd\u5fdc\u6db2\u306b\u306f\u3053\u306e\u3088\u3046\u306b\u6838\u9178\u306e\u5438\u53ce\u6ce2\u9577\u3067\u3042\u308b260 nm\u306b\u5438\u53ce\u3092\u6709\u3059\u308b\u300c\u6838\u9178\u300d\u304c\u542b\u307e\u308c\u3066\u3044\u307e\u3059\u3002\u3064\u307e\u308a\u3001PCR\u7523\u7269\u306e\u5438\u5149\u5ea6\u3092\u6e2c\u5b9a\u3057\u305f\u5834\u5408\u3001\u3053\u308c\u3089PCR\u53cd\u5fdc\u6db2\u306b\u542b\u307e\u308c\u308b\u300c\u6838\u9178\u300d\u3001\u3055\u3089\u306b\u306f\u92f3\u578b\u3068\u3057\u3066\u7528\u3044\u305fPlasmid DNA\u304c\u542b\u307e\u308c\u308b\u3053\u3068\u304b\u3089\u3001PCR\u7523\u7269\u306e\u5438\u5149\u5ea6\u3092\u6e2c\u5b9a\u3059\u308b\u3053\u3068\u306f\u3067\u304d\u307e\u305b\u3093\u3002<\/p>\n\n\n\n<p>\u3067\u306fPlasmid DNA\u3092\u6dfb\u52a0\u3057\u306a\u3044PCR\u7523\u7269\u306e\u5438\u5149\u5ea6\u30b9\u30da\u30af\u30c8\u30eb\u3068\u6bd4\u8f03\u3057\u3066\u6e2c\u5b9a\u3059\u308c\u3070\u5dee\u5206\u304b\u3089PCR\u7523\u7269\u306e\u5438\u5149\u5ea6\u3092\u6e2c\u5b9a\u3067\u304d\u308b\u3067\u3057\u3087\u3046\u304b\uff1f<\/p>\n\n\n\n<div class=\"wp-block-group is-vertical is-content-justification-center is-layout-flex wp-container-core-group-is-layout-4b2eccd6 wp-block-group-is-layout-flex\">\n<figure class=\"wp-block-image aligncenter size-full wp-image-15579\"><img loading=\"lazy\" decoding=\"async\" width=\"1267\" height=\"478\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/nanodrop-dojo-bid-ts-2-06.png\" alt=\"Plasmid DNA\u306e\u6709\u7121\u306b\u3088\u308bPCR\u7523\u7269\u306e\u5438\u5149\u5ea6\u30b9\u30da\u30af\u30c8\u30eb\u6bd4\u8f03\" class=\"wp-image-15579\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/nanodrop-dojo-bid-ts-2-06.png 1267w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/nanodrop-dojo-bid-ts-2-06-300x113.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/nanodrop-dojo-bid-ts-2-06-1024x386.png 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/nanodrop-dojo-bid-ts-2-06-768x290.png 768w\" sizes=\"auto, (max-width: 1267px) 100vw, 1267px\" \/><\/figure>\n\n\n\n<p class=\"has-text-align-center\"><strong>\u56f36\uff1aPlasmid DNA\u306e\u6709\u7121\u306b\u3088\u308bPCR\u7523\u7269\u306e\u5438\u5149\u5ea6\u30b9\u30da\u30af\u30c8\u30eb\u6bd4\u8f03<\/strong><br>\u7dd1\uff1aPlasmid DNA\u3092\u542b\u307e\u306a\u3044PCR\u7523\u7269<br>\u9ec4\uff1aPlasmid DNA\u3092\u92f3\u578b\u306b\u3057\u305fPCR\u7523\u7269<\/p>\n<\/div>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>\u7d50\u679c\u3092\u56f36\u306b\u793a\u3057\u307e\u3059\u3002Plasmid DNA\u3092\u542b\u307e\u306a\u3044PCR\u7523\u7269\u3068Plasmid DNA\u3092\u92f3\u578b\u306b\u3057\u305fPCR\u7523\u7269\u306e\u5438\u5149\u5ea6\u30b9\u30da\u30af\u30c8\u30eb\u3092\u6bd4\u8f03\u3057\u305f\u7d50\u679c\u3001Plasmid DNA\u3092\u542b\u307e\u306a\u3044PCR\u7523\u7269\u306e\u65b9\u304cPlasmid DNA\u3092\u92f3\u578b\u306b\u3057\u305fPCR\u7523\u7269\u3088\u308a\u3082260 nm\u306e\u5438\u5149\u5ea6\u306f\u9ad8\u3044\u5024\u3092\u793a\u3057\u307e\u3057\u305f\u3002\u3053\u308c\u306f\u3001\u304a\u305d\u3089\u304f\u53cd\u5fdc\u6db2\u306b\u542b\u307e\u308c\u308bPCR\u30de\u30b9\u30bf\u30fc\u30df\u30c3\u30af\u30b9\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u6dfb\u52a0\u91cf\u304c\u308f\u305a\u304b\u306b\u30b5\u30f3\u30d7\u30eb\u9593\u3067\u7570\u306a\u308b\u3053\u3068\u3067\u3053\u306e\u3088\u3046\u306a\u7d50\u679c\u3068\u306a\u3063\u305f\u3068\u793a\u5506\u3055\u308c\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u3064\u307e\u308aPlasmid DNA\u3092\u542b\u307e\u306a\u3044PCR\u7523\u7269\u3092\u6bd4\u8f03\u5bfe\u8c61\u3068\u3057\u3066PCR\u7523\u7269\u306e\u5438\u5149\u5ea6\u3092\u6c42\u3081\u3088\u3046\u3068\u3059\u308b\u5834\u5408\u3001\u975e\u5e38\u306b\u6b63\u78ba\u306a\u30d4\u30da\u30c3\u30c8\u64cd\u4f5c\u304c\u6c42\u3081\u3089\u308c\u3001\u304b\u3064\u540c\u7b49\u91cf\u304c\u542b\u307e\u308c\u308b\u304b\u3069\u3046\u304b\u3092\u78ba\u8a8d\u3059\u308b\u3053\u3068\u306f\u96e3\u3057\u304f\u3001\u4ee5\u4e0a\u306e\u3053\u3068\u304b\u3089\u3053\u306e\u3088\u3046\u306a\u65b9\u6cd5\u3067\u306ePCR\u7523\u7269\u306e\u5438\u5149\u5ea6\u6e2c\u5b9a\u306f\u73fe\u5b9f\u7684\u3067\u306f\u306a\u3044\u3068\u8003\u3048\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u3067\u306fPCR\u7523\u7269\u306e\u5438\u5149\u5ea6\u60c5\u5831\u304c\u5fc5\u8981\u306a\u5834\u5408\u3069\u306e\u3088\u3046\u306b\u3059\u308c\u3070\u3044\u3044\u3067\u3057\u3087\u3046\u304b\uff1f<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-pcr\u7523\u7269\u306e\u7cbe\u88fd\u306fpcr\u5897\u5e45\u7523\u7269\u306e\u6e2c\u5b9a\u306b\u6709\u52b9\u304b\">PCR\u7523\u7269\u306e\u7cbe\u88fd\u306fPCR\u5897\u5e45\u7523\u7269\u306e\u6e2c\u5b9a\u306b\u6709\u52b9\u304b\uff1f<\/h2>\n\n\n\n<p>\u30d7\u30e9\u30a4\u30de\u30fc\u306f20 bp\u7a0b\u5ea6\u306e\u9577\u3055\u304b\u3089\u306a\u308b\u6838\u9178\u3001dNTP mix\u306f\u30e2\u30ce\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\u3067\u3042\u308a\u3001PCR\u7523\u7269\u306e\u7cbe\u88fd\u3067\u3053\u308c\u3089\u593e\u96d1\u7269\u3092\u9664\u304f\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002\u3053\u3053\u3067\u306f\u3001Invitorogen\u2122 PureLink\u2122 PCR Purification Kit\uff08\u88fd\u54c1\u756a\u53f7\uff1aK310001\uff09\u3092\u7528\u3044\u3066PCR\u7523\u7269\u306e\u7cbe\u88fd\u3092\u5b9f\u65bd\u3057\u3001\u518d\u5ea6\u3001\u5438\u5149\u5ea6\u3092\u6e2c\u5b9a\u3057\u307e\u3057\u305f\u3002<\/p>\n\n\n\n<div class=\"wp-block-group is-vertical is-content-justification-center is-layout-flex wp-container-core-group-is-layout-4b2eccd6 wp-block-group-is-layout-flex\">\n<figure class=\"wp-block-image aligncenter size-full wp-image-15580\"><img loading=\"lazy\" decoding=\"async\" width=\"1080\" height=\"260\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/nanodrop-dojo-bid-ts-2-07.png\" alt=\"PCR\u7523\u7269\u306e\u7cbe\u88fd\" class=\"wp-image-15580\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/nanodrop-dojo-bid-ts-2-07.png 1080w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/nanodrop-dojo-bid-ts-2-07-300x72.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/nanodrop-dojo-bid-ts-2-07-1024x247.png 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/nanodrop-dojo-bid-ts-2-07-768x185.png 768w\" sizes=\"auto, (max-width: 1080px) 100vw, 1080px\" \/><\/figure>\n\n\n\n<p class=\"has-text-align-center\"><strong>\u56f37\uff1aPCR\u7523\u7269\u306e\u7cbe\u88fd<\/strong><br>\u24f5\u7cbe\u88fd\u524dPlasmid DNA\u92f3\u578bPCR\u7523\u7269 \u24f6\u7cbe\u88fd\u524dPlasmid DNA\u3092\u542b\u307e\u306a\u3044PCR\u7523\u7269<br>\u24f7\u7cbe\u88fd\u5f8cPlasmid DNA\u92f3\u578bPCR\u7523\u7269 \u24f8\u7cbe\u88fd\u5f8cPlasmid DNA\u3092\u542b\u307e\u306a\u3044PCR\u7523\u7269<\/p>\n<\/div>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<figure class=\"wp-block-image aligncenter size-full wp-image-15581\"><img loading=\"lazy\" decoding=\"async\" width=\"1235\" height=\"447\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/nanodrop-dojo-bid-ts-2-08.png\" alt=\"\u7cbe\u88fd\u5f8cPlasmid DNA\u92f3\u578bPCR\u7523\u7269\u306e\u5438\u5149\u5ea6\u30b9\u30da\u30af\u30c8\u30eb\" class=\"wp-image-15581\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/nanodrop-dojo-bid-ts-2-08.png 1235w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/nanodrop-dojo-bid-ts-2-08-300x109.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/nanodrop-dojo-bid-ts-2-08-1024x371.png 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/nanodrop-dojo-bid-ts-2-08-768x278.png 768w\" sizes=\"auto, (max-width: 1235px) 100vw, 1235px\" \/><figcaption class=\"wp-element-caption\">\u56f38:\u7cbe\u88fd\u5f8cPlasmid DNA\u92f3\u578bPCR\u7523\u7269\u306e\u5438\u5149\u5ea6\u30b9\u30da\u30af\u30c8\u30eb<\/figcaption><\/figure>\n\n\n\n<p>\u7cbe\u88fd\u3055\u308c\u305fPlasmid DNA\u3092\u92f3\u578b\u306b\u3057\u305fPCR\u7523\u7269\u3092\u6e2c\u5b9a\u3057\u305f\u7d50\u679c\u3001\u5f97\u3089\u308c\u308b260 nm\u306e\u5438\u5149\u5ea6\u306f\u4f4e\u3044\u3067\u3059\u304cPCR\u5897\u5e45\u3055\u308c\u305f\u300c\u6838\u9178\u300d\u3092\u5438\u5149\u5ea6\u30b9\u30da\u30af\u30c8\u30eb\u304b\u3089\u306f\u3042\u308b\u7a0b\u5ea6\u5224\u65ad\u3067\u304d\u308b\u3053\u3068\u304c\u793a\u3055\u308c\u307e\u3057\u305f\u3002<br>\u4eca\u56de\u306e\u5834\u5408\u300150 \u03bcL\u53cd\u5fdc\u7523\u7269\u306e\u6fc3\u5ea6\u304c2.2 ng\/\u03bcL\u3067\u3042\u308a\u3001\u304a\u304a\u3088\u305d100 ng\u306ePCR\u7523\u7269\u304c\u5897\u5e45\u3055\u308c\u305f\u3053\u3068\u304c\u63a8\u6e2c\u3055\u308c\u307e\u3059\u3002\u5f93\u3063\u3066\u3001PCR\u7523\u7269\u306e\u6fc3\u5ea6\u6e2c\u5b9a\u304c\u5fc5\u8981\u306a\u5834\u5408\u3001PCR\u7523\u7269\u306e\u7cbe\u88fd\u3092\u5b9f\u65bd\u3057\u3066\u304b\u3089\u5438\u5149\u5ea6\u3092\u6e2c\u5b9a\u3059\u308b\u3053\u3068\u304c\u5fc5\u8981\u306b\u306a\u308a\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u7406\u8ad6\u4e0a\u306f\u3001PCR\u7523\u7269\u4e2d\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u306a\u3069\u306e\u300c\u6838\u9178\u300d\u3092\u9664\u304f\u3053\u3068\u3067\u5897\u5e45\u3055\u308c\u305f\u6838\u9178\u3092\u78ba\u8a8d\u3067\u304d\u308b\u3068\u8003\u3048\u307e\u3059\u3002PCR\u53cd\u5fdc\u306e\u5897\u5e45\u306f\u3042\u304f\u307e\u3067\u3082\u30b3\u30d4\u30fc\u6570\uff08\u5206\u5b50\u6fc3\u5ea6\uff09\u306e\u5897\u5e45\u3067\u3042\u308a\u3001\u30b5\u30f3\u30d7\u30eb\u6fc3\u5ea6\u306e\u5897\u5e45\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u3088\u3063\u3066\u7cbe\u88fd\u5f8c\u306e\u6eb6\u5a92\u91cf\u306b\u3088\u3063\u3066\u306f260 nm\u306e\u5438\u5149\u5ea6\u304c\u4f4e\u3044\u3053\u3068\u304c\u3042\u308a\u3001\u5438\u5149\u5ea6\u306e\u4fe1\u983c\u6e2c\u5b9a\u57df\u306e\u95a2\u4fc2\u4e0a\u3001\u6b63\u78ba\u306a\u5438\u5149\u5ea6\u6e2c\u5b9a\u306f\u56f0\u96e3\u306a\u5834\u5408\u3082\u3042\u308a\u307e\u3059\u3002\u3055\u3089\u306b\u306fPCR\u53cd\u5fdc\u7528\u306e\u92f3\u578b\u306b\u4f7f\u3063\u305fPlasmid DNA\u306fPCR\u7523\u7269\u306e\u7cbe\u88fd\u5f8c\u3082\u9664\u53bb\u3055\u308c\u307e\u305b\u3093\u3002\u4ee5\u4e0a\u306e\u3053\u3068\u304b\u3089PCR\u7523\u7269\u3092\u7cbe\u88fd\u3057\u3066\u3082PCR\u7523\u7269\u306e\u307f\u306e\u5438\u5149\u5ea6\u306f\u6e2c\u5b9a\u3067\u304d\u305a\u3001\u3042\u304f\u307e\u3067\u3082\u92f3\u578b\u306b\u4f7f\u7528\u3057\u305fPlasmid DNA\u3092\u542b\u3080\u63a8\u6e2c\u5024\u3068\u306a\u308b\u70b9\u3001\u6ce8\u610f\u304c\u5fc5\u8981\u306b\u306a\u308a\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u7cbe\u88fd\u3092\u884c\u3046\u3053\u3068\u306a\u304f\u3001PCR\u7523\u7269\u306e\u6fc3\u5ea6\u3092\u6e2c\u5b9a\u3059\u308b\u65b9\u6cd5\u3068\u3057\u3066\u3001\u4e00\u822c\u7684\u306b\u306f\u6fc3\u5ea6\u65e2\u77e5\u306eDNA\u30b5\u30a4\u30ba\u30de\u30fc\u30ab\u30fc\u3068PCR\u7523\u7269\u3092\u4e00\u7dd2\u306b\u6cf3\u52d5\u3059\u308b\u3053\u3068\u3067\u5f97\u3089\u308c\u308b\u6cf3\u52d5\u50cf\u304b\u3089\u3001\u30c7\u30f3\u30b7\u30c8\u30e1\u30fc\u30bf\u30fc\u3092\u7528\u3044\u3066\u304a\u304a\u3088\u305d\u306ePCR\u7523\u7269\u306e\u91cd\u91cf\u3092\u4e88\u6e2c\u3059\u308b\u3053\u3068\u3082\u53ef\u80fd\u3067\u3059\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u307e\u3068\u3081\">\u307e\u3068\u3081<\/h2>\n\n\n\n<p>\u4eca\u56de\u306f\u3001PCR\u7523\u7269\u306e\u5438\u5149\u5ea6\u6e2c\u5b9a\u306b\u3064\u3044\u3066\u3054\u7d39\u4ecb\u3057\u307e\u3057\u305f\u3002\u5b9f\u969b\u306bPCR\u7523\u7269\u3068\u3044\u3046\u300c\u6838\u9178\u300d\u3067\u3042\u3063\u3066\u3082\u53cd\u5fdc\u6db2\u4e2d\u306b\u3055\u307e\u3056\u307e\u306a\u6838\u9178\u593e\u96d1\u7269\u304c\u5b58\u5728\u3059\u308b\u3053\u3068\u3067\u3001\u305d\u306e\u307e\u307e\u3067\u306fPCR\u7523\u7269\u306e\u5438\u5149\u5ea6\u3092\u6e2c\u5b9a\u3067\u304d\u306a\u3044\u3053\u3068\u304c\u304a\u5206\u304b\u308a\u3044\u305f\u3060\u3051\u305f\u304b\u3068\u601d\u3044\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u3053\u306e\u30b7\u30ea\u30fc\u30ba\u3067\u306fNanoDrop\u5fae\u91cf\u5206\u5149\u5149\u5ea6\u8a08\u306b\u3088\u308b\u5438\u5149\u5ea6\u6e2c\u5b9a\u306b\u95a2\u308f\u308b\u60c5\u5831\u3084Tips\u3092\u3054\u6848\u5185\u3057\u3066\u3044\u304f\u4e88\u5b9a\u3067\u3059\u3002\u4ee5\u4e0b\u30ea\u30f3\u30af\u3088\u308a\u300cNanoDrop\u300d\u306b\u95a2\u9023\u3059\u308b\u30d6\u30ed\u30b0\u8a18\u4e8b\u3092\u3054\u89a7\u3044\u305f\u3060\u3051\u307e\u3059\u3002<br><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/tag\/nanodrop\/?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_nanodrop_dojo_bid_ts_2_Social_LAB\" target=\"_blank\" rel=\"noopener\">https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/tag\/nanodrop\/<\/a><\/p>\n\n\n\n<p>NanoDrop\u3054\u5229\u7528\u306e\u304a\u5ba2\u69d8\u3078<br>Web\u30b5\u30a4\u30c8\u306e\u30ea\u30bd\u30fc\u30b9\u30da\u30fc\u30b8\uff08<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/industrial\/spectroscopy-elemental-isotope-analysis\/molecular-spectroscopy\/uv-vis-spectrophotometry\/instruments\/nanodrop\/resources.html?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_nanodrop_dojo_bid_ts_2_Social_LAB\" target=\"_blank\" rel=\"noopener\">thermofisher.com\/jp-nanodrop-setup<\/a>\uff09\u306b\u306f\u3001How-to\u52d5\u753b\u306a\u3069NanoDrop One \/ OneC\u306b\u95a2\u3059\u308b\u69d8\u3005\u306a\u304a\u5f79\u7acb\u3061\u30b3\u30f3\u30c6\u30f3\u30c4\u3092\u63b2\u8f09\u3057\u3066\u3044\u307e\u3059\u3002<br>\u4f75\u305b\u3066\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002<br>\u307e\u305fNanoDrop\u5fae\u91cf\u5206\u5149\u5149\u5ea6\u8a08\u30b7\u30ea\u30fc\u30ba\u306b\u3054\u8208\u5473\u306e\u30e6\u30fc\u30b6\u30fc\u3055\u307e\u306f\u4ee5\u4e0b\u30ea\u30f3\u30af\u3088\u308a\u30c7\u30e2\u306e\u3054\u4f9d\u983c\u3082\u53ef\u80fd\u3067\u3059\u306e\u3067\u3054\u6d3b\u7528\u3044\u305f\u3060\u3051\u308c\u3070\u3068\u601d\u3044\u307e\u3059\u3002<\/p>\n\n\n\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/nanodrop-inquiry-form.html?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_nanodrop_dojo_bid_ts_2_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">\u304a\u554f\u3044\u5408\u308f\u305b\u306f\u3053\u3061\u3089<\/a><\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-\u9023\u8f09nanodrop\u9053\u5834\">\u9023\u8f09NanoDrop\u9053\u5834<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/nanodrop-dojo-bid-ts-1\/?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_nanodrop_dojo_bid_ts_2_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u7b2c1\u56de \u5438\u5149\u5ea6\u6e2c\u5b9a\u306b\u3088\u308b\u593e\u96d1\u7269\u306e\u5f71\u97ff\u3092\u8003\u3048\u3088\u3046\uff01<\/a><\/li>\n\n\n\n<li>\u7b2c2\u56de \u3044\u308d\u3044\u308d\u306a\u6838\u9178\u30b5\u30f3\u30d7\u30eb\u306e\u5438\u5149\u5ea6\u3092\u6e2c\u5b9a\u3057\u3088\u3046\uff01\uff5ePCR\u7523\u7269\uff5e<\/li>\n\n\n\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/nanodrop-dojo-bid-ts-3\/?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_nanodrop_dojo_bid_ts_2_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u7b2c3\u56de 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class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u7528\u306b\u306f\u4f7f\u7528\u3044\u305f\u3060\u3051\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>NanoDrop\u3092\u7528\u3044\u305f\u6838\u9178\u30b5\u30f3\u30d7\u30eb\u306e\u5438\u5149\u5ea6\u6e2c\u5b9a\u306b\u3088\u308a\u3001\u5f97\u3089\u308c\u308b\u5438\u5149\u5ea6\u60c5\u5831\u304b\u3089\u6838\u9178\u6fc3\u5ea6\u3084\u593e\u96d1\u7269\u306e\u6709\u7121\u3092\u78ba\u8a8d\u3067\u304d\u307e\u3059\u3002\u3057\u304b\u3057\u3069\u306e\u3088\u3046\u306a\u6838\u9178\u30b5\u30f3\u30d7\u30eb\u306a\u3089\u6e2c\u5b9a\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u306a\u306e\u3067\u3057\u3087\u3046\u304b\uff1f 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