{"id":15605,"date":"2023-10-31T15:26:00","date_gmt":"2023-10-31T06:26:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/?p=15605"},"modified":"2025-01-21T15:07:29","modified_gmt":"2025-01-21T06:07:29","slug":"western-blot-protein-ts-7","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/western-blot-protein-ts-7\/","title":{"rendered":"\u5316\u5b66\u767a\u5149\u3068\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u691c\u51fa\u306e\u9078\u629e\u30dd\u30a4\u30f3\u30c8\uff1a\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u9078\u3073\u65b9\u3000\u7b2c7\u56de"},"content":{"rendered":"<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-13122\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/12\/western.jpg\" alt=\"westernblotting\" width=\"761\" height=\"476\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/12\/western.jpg 761w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/12\/western-300x188.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/12\/western-320x200.jpg 320w\" sizes=\"auto, (max-width: 761px) 100vw, 761px\" \/>\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u300c\u9078\u3073\u65b9\u300d\u306b\u3064\u3044\u3066\u9023\u8f09\u3057\u3066\u3044\u308b\u672c\u30b7\u30ea\u30fc\u30ba\u3001<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/western-blot-protein-ts-6\/?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u524d\u56de<\/a>\u306f\u6297\u4f53\u306b\u3064\u3044\u3066\u3054\u7d39\u4ecb\u3057\u307e\u3057\u305f\u3002\u6b21\u306f\u3044\u3088\u3044\u3088\u691c\u51fa\u306e\u30b9\u30c6\u30c3\u30d7\u3067\u3059\u3002\u9577\u3044\u5de5\u7a0b\u3092\u7d4c\u3066\u3001\u3088\u3046\u3084\u304f\u30d0\u30f3\u30c9\u304c\u78ba\u8a8d\u3067\u304d\u307e\u3059\u3002<br \/>\n\u672c\u30d6\u30ed\u30b0\u3067\u306f\u3001\u691c\u51fa\u8a66\u85ac\u3092\u9078\u3076\u3068\u304d\u306b\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\u30dd\u30a4\u30f3\u30c8\u306b\u3064\u3044\u3066\u3001\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n<h2>\u767a\u8272\u691c\u51fa<\/h2>\n<p>\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u691c\u51fa\u65b9\u6cd5\u306f\u3001\u5927\u307e\u304b\u306b\u767a\u8272\u691c\u51fa\u3001\u5316\u5b66\u767a\u5149\u691c\u51fa\u3001\u86cd\u5149\u691c\u51fa\u306b\u5206\u304b\u308c\u307e\u3059\u3002<br \/>\n\u305d\u308c\u305e\u308c\u3001\u3069\u306e\u3088\u3046\u306a\u3068\u304d\u306b\u9078\u629e\u3059\u308b\u306e\u304b\u3001\u88681\u3092\u898b\u3066\u307f\u307e\u3057\u3087\u3046\u3002<\/p>\n<table>\n<caption><b>\u88681. \u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u691c\u51fa\u65b9\u6cd5<\/b><\/caption>\n<tbody>\n<tr>\n<td width=\"80\"><b>\u691c\u51fa\u65b9\u6cd5<\/b><\/td>\n<td width=\"160\"><b>\u691c\u51fa\u306e\u6d41\u308c<\/b><\/td>\n<td width=\"210\"><b>\u3069\u3046\u3044\u3046\u3068\u304d\u306b\u4f7f\u7528\uff1f<\/b><\/td>\n<td width=\"151\"><b>\u30c7\u30e1\u30ea\u30c3\u30c8<\/b><\/td>\n<\/tr>\n<tr>\n<td width=\"80\">\u767a\u8272\u691c\u51fa<\/td>\n<td width=\"160\">\u9175\u7d20\u6a19\u8b58\u4e8c\u6b21\u6297\u4f53\u306b\u5bfe\u3057\u3066\u3001\u767a\u8272\u7528\u306e\u57fa\u8cea\u3092\u7528\u3044\u308b<\/td>\n<td width=\"210\">\u767a\u5149\u3084\u86cd\u5149\u306e\u691c\u51fa\u5668\u304c\u306a\u3044\u3068\u304d\u3084\u3001\u76ee\u8996\u3067\u30d0\u30f3\u30c9\u3092\u78ba\u8a8d\u3057\u305f\u3044\u3068\u304d<\/td>\n<td width=\"151\">\u611f\u5ea6\u304c\u4f4e\u3044\u3053\u3068\u304c\u591a\u3044<\/td>\n<\/tr>\n<tr>\n<td width=\"80\">\u5316\u5b66\u767a\u5149<br \/>\n\u691c\u51fa<\/td>\n<td width=\"160\">\u9175\u7d20\u6a19\u8b58\u4e8c\u6b21\u6297\u4f53\u306b\u5bfe\u3057\u3001\u5316\u5b66\u767a\u5149\u7528\u306e\u57fa\u8cea\u3092\u7528\u3044\u308b<\/td>\n<td width=\"210\">\u9ad8\u3044\u611f\u5ea6\u304c\u5fc5\u8981\u306a\u3068\u304d<\/td>\n<td width=\"151\">CCD\u30a4\u30e1\u30fc\u30b8\u30e3\u30fc\u3084X\u7dda\u30d5\u30a3\u30eb\u30e0\u306a\u3069\u3067\u691c\u51fa\u304c\u5fc5\u8981<\/td>\n<\/tr>\n<tr>\n<td width=\"80\">\u86cd\u5149\u691c\u51fa<\/td>\n<td width=\"160\">\u86cd\u5149\u8272\u7d20\u3092\u6a19\u8b58\u3057\u305f\u4e8c\u6b21\u6297\u4f53\u3092\u7528\u3044\u308b<\/td>\n<td width=\"210\">\u8907\u6570\u306e\u7570\u306a\u308b\u30d0\u30f3\u30c9\u3092\u540c\u6642\u306b\u691c\u51fa\u3057\u305f\u3044\u3068\u304d<\/td>\n<td width=\"151\">\u86cd\u5149\u691c\u51fa\u5668\u304c\u5fc5\u8981<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>\u307e\u305a\u306f\u6700\u3082\u6b74\u53f2\u304c\u53e4\u3044\u767a\u8272\u691c\u51fa\u304b\u3089\u3001\u8a73\u3057\u304f\u8aac\u660e\u3057\u307e\u3057\u3087\u3046\u3002<\/p>\n<p>\uff1c\u767a\u8272\u691c\u51fa\uff1e<br \/>\n\u767a\u8272\u691c\u51fa\u306f\u3001\u540d\u524d\u306e\u3068\u304a\u308a\u30e1\u30f3\u30d6\u30ec\u30f3\u4e0a\u3067\u767a\u8272\u3055\u305b\u3066\u30bf\u30fc\u30b2\u30c3\u30c8\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u691c\u51fa\u3059\u308b\u65b9\u6cd5\u3067\u3059\u3002\u57fa\u8cea\u306b\u3088\u308a\u611f\u5ea6\u304c\u7570\u306a\u308a\u307e\u3059\u304c\u3001\u305f\u3044\u3066\u3044\u306e\u5834\u5408\u3001\u5316\u5b66\u767a\u5149\u3068\u6bd4\u3079\u308b\u3068\u611f\u5ea6\u306f\u52a3\u308a\u307e\u3059\u3002<br \/>\n\u3057\u304b\u3057\u3001\u6a5f\u5668\u3092\u7528\u3044\u305a\u306b\u30d0\u30f3\u30c9\u3092\u53ef\u8996\u3067\u78ba\u8a8d\u3067\u304d\u308b\u5927\u304d\u306a\u30e1\u30ea\u30c3\u30c8\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<p>\u88682\u3068\u88683\u306b\u767a\u8272\u8a66\u85ac\u3092\u4e00\u89a7\u306b\u307e\u3068\u3081\u307e\u3057\u305f\u3002\u4e8c\u6b21\u6297\u4f53\u306bHRP\uff08\u897f\u6d0b\u308f\u3055\u3073\u30da\u30eb\u30aa\u30ad\u30b7\u30c0\u30fc\u30bc\uff09\u6a19\u8b58\u4e8c\u6b21\u6297\u4f53\u3092\u7528\u3044\u305f\u5834\u5408\u306f\u3001\u767a\u8272\u57fa\u8cea\u3068\u3057\u3066DAB\u3084TMB\u3001\u4e8c\u6b21\u6297\u4f53\u306bAP\uff08\u30a2\u30eb\u30ab\u30ea\u30d5\u30a9\u30b9\u30d5\u30a1\u30bf\u30fc\u30bc\uff09\u6a19\u8b58\u4e8c\u6b21\u6297\u4f53\u3092\u7528\u3044\u305f\u5834\u5408\u306f\u3001\u767a\u8272\u57fa\u8cea\u3068\u3057\u3066NBT\u3084BCIP\u306a\u3069\u304c\u3042\u308a\u307e\u3059\u3002\u57fa\u8cea\u306b\u3088\u3063\u3066\u8272\u3084\u611f\u5ea6\u304c\u7570\u306a\u308a\u307e\u3059\u306e\u3067\u3001\u88682\u30013\u306e\u60c5\u5831\u3092\u5143\u306b\u9078\u3073\u307e\u3057\u3087\u3046\u3002<\/p>\n<table width=\"620\">\n<caption><b>\u88682. HRP\u7528\u767a\u8272\u8a66\u85ac\uff08\u57fa\u8cea\uff09<\/b><\/caption>\n<tbody>\n<tr>\n<td width=\"241\"><b>\u767a\u8272\u7528\u691c\u51fa\u8a66\u85ac<\/b><\/td>\n<td width=\"87\"><b>\u8272<\/b><\/td>\n<td width=\"83\"><b>\u691c\u51fa\u9650\u754c<\/b><\/td>\n<td width=\"209\"><b>\u63a8\u5968\u306e\u6297\u4f53\u5e0c\u91c8\u500d\u7387<sup>\u203b<\/sup><\/b><\/td>\n<\/tr>\n<tr>\n<td width=\"241\">Thermo Scientific\u2122<br \/>\n<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/34065?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">Metal Enhanced DAB Substrate Kit<\/a><\/td>\n<td width=\"87\">\u8336\uff5e\u9ed2<\/td>\n<td width=\"83\">17 pg<\/td>\n<td width=\"209\">1\u00b0: 1:1,000<br \/>\n2\u00b0: 1:5,000\uff5e1:50,000<\/td>\n<\/tr>\n<tr>\n<td width=\"241\">Thermo Scientific\u2122<br \/>\n<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/37574?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">1-Step\u2122 Ultra TMB Blotting Solution<\/a><\/td>\n<td width=\"87\">\u6697\u9752<\/td>\n<td width=\"83\">20 pg<\/td>\n<td width=\"209\">1\u00b0: 1:1,000<br \/>\n2\u00b0: 1:5,000\uff5e1:10,000<\/td>\n<\/tr>\n<tr>\n<td width=\"241\">Thermo Scientific\u2122<br \/>\n<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/34000?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">Pierce\u2122 CN\/DAB Substrate Kit<\/a><\/td>\n<td width=\"87\">\u9ed2<\/td>\n<td width=\"83\">500 pg<\/td>\n<td width=\"209\">1\u00b0: 1:500<br \/>\n2\u00b0: 1:2,000\uff5e1:20,000<\/td>\n<\/tr>\n<tr>\n<td width=\"241\">Thermo Scientific\u2122<br \/>\n<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/34002?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">Pierce\u2122 DAB Substrate Kit<\/a><\/td>\n<td width=\"87\">\u8336<\/td>\n<td width=\"83\">1 ng<\/td>\n<td width=\"209\">1\u00b0: 1:500<br \/>\n2\u00b0: 1:2,000\uff5e1:20,000<\/td>\n<\/tr>\n<tr>\n<td width=\"241\">Thermo Scientific\u2122 <a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/34018?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">1-Step\u2122 TMB-Blotting Substrate Solution<\/a><\/td>\n<td width=\"87\">\u6697\u9752<\/td>\n<td width=\"83\">1 ng<\/td>\n<td width=\"209\">1\u00b0: 1:500<br \/>\n2\u00b0: 1:2,000\uff5e1:20,000<\/td>\n<\/tr>\n<tr>\n<td width=\"241\">Thermo Scientific\u2122 <a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/34012?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">1-Step\u2122 Chloronaphthol Substrate Solution<\/a><\/td>\n<td width=\"87\">\u9752\uff5e\u7d2b<\/td>\n<td width=\"83\">5 ng<\/td>\n<td width=\"209\">1\u00b0: 1:500<br \/>\n2\u00b0: 1:2,000\uff5e1:20,000<\/td>\n<\/tr>\n<\/tbody>\n<tfoot>\n<tr>\n<td colspan=\"4\"><span style=\"font-size: 12px\">\u203b\u6297\u4f531 mg\/mL\u3092\u7528\u3044\u305f\u5834\u5408\u306e\u5e0c\u91c8\u500d\u7387\u3067\u3059\u3002<\/span><\/td>\n<\/tr>\n<\/tfoot>\n<\/table>\n<table width=\"654\">\n<caption><b>\u88683. AP\u7528\u767a\u8272\u57fa\u8cea<\/b><\/caption>\n<tbody>\n<tr>\n<td width=\"200\"><b>\u767a\u8272\u7528\u691c\u51fa\u8a66\u85ac<\/b><\/td>\n<td width=\"66\"><b>\u8272<\/b><\/td>\n<td width=\"76\"><b>\u691c\u51fa\u9650\u754c<\/b><\/td>\n<td width=\"161\"><b>\u63a8\u5968\u306e\u6297\u4f53\u5e0c\u91c8\u500d\u7387<sup>\u203b<\/sup><\/b><\/td>\n<td width=\"151\"><b>\u6ce8\u610f\u4e8b\u9805<\/b><\/td>\n<\/tr>\n<tr>\n<td width=\"200\">Thermo Scientific\u2122 <a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/34042?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">1-Step\u2122 NBT\/BCIP Substrate Solution<\/a><\/td>\n<td width=\"66\">\u9ed2\uff5e\u7d2b<\/td>\n<td width=\"76\">30 pg<\/td>\n<td width=\"161\">1\u00b0: 1:500<br \/>\n2\u00b0: 1:5,000\uff5e1:50,000<\/td>\n<td width=\"151\">\u30d0\u30c3\u30d5\u30a1\u30fc\u3068\u3057\u3066PBS\u306f\u4f7f\u7528\u3067\u304d\u306a\u3044<\/td>\n<\/tr>\n<\/tbody>\n<tfoot>\n<tr>\n<td colspan=\"4\"><span style=\"font-size: 12px\">\u203b\u6297\u4f531 mg\/mL\u3092\u7528\u3044\u305f\u5834\u5408\u306e\u5e0c\u91c8\u500d\u7387\u3067\u3059\u3002<\/span><\/td>\n<\/tr>\n<\/tfoot>\n<\/table>\n<div id=\"attachment_15609\" style=\"width: 333px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-15609\" class=\"size-full wp-image-15609\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-01.png\" alt=\"Thermo Scientific\u2122 1-Step\u2122 Ultra TMB-Blotting Solution\u3067\u691c\u51fa\u3057\u305f\u7d50\u679c \u30b5\u30f3\u30d7\u30eb\uff1aHeLa Cell Lysate\u3001\u4e00\u6b21\u6297\u4f53\" width=\"323\" height=\"322\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-01.png 323w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-01-300x300.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-01-150x150.png 150w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-01-80x80.png 80w\" sizes=\"auto, (max-width: 323px) 100vw, 323px\" \/><p id=\"caption-attachment-15609\" class=\"wp-caption-text\">\u56f31. Thermo Scientific\u2122 1-Step\u2122 Ultra TMB-Blotting Solution\u3067\u691c\u51fa\u3057\u305f\u7d50\u679c<br \/>\u30b5\u30f3\u30d7\u30eb\uff1aHeLa Cell Lysate\u3001\u4e00\u6b21\u6297\u4f53\uff1aInvitrogen\u2122 Heat Shock Protein 86 Polyclonal Antibody \uff08Hsp86\uff09<\/p><\/div>\n<h2>\u5316\u5b66\u767a\u5149\u691c\u51fa<\/h2>\n<p>\u73fe\u5728\u3001\u6700\u3082\u3088\u304f\u5229\u7528\u3055\u308c\u3066\u3044\u308b\u306e\u304c\u3001\u3053\u306e\u5316\u5b66\u767a\u5149\u691c\u51fa\u3067\u3059\u3002\u7279\u306b\u4e8c\u6b21\u6297\u4f53\u306bHRP\uff08\u897f\u6d0b\u308f\u3055\u3073\u30d1\u30fc\u30aa\u30ad\u30b7\u30c0\u30fc\u30bc\uff09\u3092\u6a19\u8b58\u3055\u305b\u3066\u691c\u51fa\u3059\u308b\u3053\u3068\u304c\u591a\u3044\u3067\u3059\u3002HRP\u3068\u904e\u9178\u5316\u6c34\u7d20\u306e\u5b58\u5728\u4e0b\u3067\u30eb\u30df\u30ce\u30fc\u30eb\u304c\u9178\u5316\u3055\u308c\u308b\u3068\u30013-\u30a2\u30df\u30ce\u30d5\u30bf\u30eb\u9178\u3068\u3044\u3046\u52b1\u8d77\u72b6\u614b\u306e\u751f\u6210\u7269\u304c\u7b97\u51fa\u3055\u308c\u307e\u3059\u3002\u3053\u308c\u304c\u57fa\u5e95\u72b6\u614b\u306b\u623b\u308b\u3068\u304d\u306b\u767a\u3059\u308b\u5149\u304c\u300c\u5316\u5b66\u767a\u5149\u300d\u3067\u3059\u3002\u3053\u306e\u5316\u5b66\u7684\u306a\u767a\u5149\u73fe\u8c61\u306e\u3053\u3068\u3092\u5316\u5b66\u767a\u5149\u3001\u307e\u305f\u306f\u30b1\u30df\u30eb\u30df\u30cd\u30c3\u30bb\u30f3\u30b9\uff08Chemiluminescence\uff09\u3001\u7565\u3057\u3066\u300c\u30b1\u30df\u30eb\u30df\u300d\u306a\u3069\u3068\u547c\u3070\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>\u5316\u5b66\u767a\u5149\u691c\u51fa\u8a66\u85ac\u306f\u3001\u4e00\u822c\u7684\u306b\u767a\u8272\u8a66\u85ac\u3088\u308a\u3082\u611f\u5ea6\u304c\u9ad8\u3044\u3067\u3059\u3002\u88684\u30015\u306b\u5404\u57fa\u8cea\u306e\u611f\u5ea6\u3084\u767a\u5149\u6301\u7d9a\u6642\u9593\u306a\u3069\u3092\u307e\u3068\u3081\u307e\u3057\u305f\u3002<\/p>\n<table width=\"654\">\n<caption><b>\u88684. HRP\u7528\u3000\u5316\u5b66\u767a\u5149\u691c\u51fa\u8a66\u85ac\uff08\u57fa\u8cea\uff09<\/b><\/caption>\n<tbody>\n<tr>\n<td width=\"177\"><b>\u5316\u5b66\u767a\u5149\u691c\u51fa\u8a66\u85ac<\/b><\/td>\n<td width=\"75\"><b>\u7565\u79f0<\/b><\/td>\n<td width=\"71\"><b>\u30b7\u30b0\u30ca\u30eb\u6301\u7d9a\u6642\u9593<\/b><\/td>\n<td width=\"162\"><b>\u691c\u51fa\u9650\u754c<\/b><\/td>\n<td width=\"169\"><b>\u63a8\u5968\u306e\u6297\u4f53\u5e0c\u91c8\u500d\u7387<sup>\u203b<\/sup><\/b><\/td>\n<\/tr>\n<tr>\n<td width=\"177\">Thermo Scientific\u2122 <a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/A38554?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">SuperSignal\u2122 West Atto<br \/>\nUltimate Sensitivity<br \/>\nSubstrate<\/a><\/td>\n<td width=\"75\">West Atto<\/td>\n<td width=\"71\">6\u6642\u9593<\/td>\n<td width=\"162\">\u30d5\u30a7\u30e0\u30c8\u30b0\u30e9\u30e0\u4f4e\u57df\uff5e\u30a2\u30c8\u30b0\u30e9\u30e0\u9ad8\u57df<\/td>\n<td width=\"169\">1\u00b0: 1:5,000<br \/>\n2\u00b0: 1:100,000\uff5e 1:250,000<\/td>\n<\/tr>\n<tr>\n<td width=\"177\">Thermo Scientific\u2122 <a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/34094?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">SuperSignal\u2122 West Femto<br \/>\nMaximum Sensitivity<br \/>\nSubstrate<\/a><\/td>\n<td width=\"75\">West Femto<\/td>\n<td width=\"71\">8\u6642\u9593<\/td>\n<td width=\"162\">\u30d5\u30a7\u30e0\u30c8\u30b0\u30e9\u30e0\u4f4e\uff5e\u4e2d\u57df<\/td>\n<td width=\"169\">1\u00b0: 1:5,000<br \/>\n2\u00b0: 1:100,000\uff5e 1:500,000<\/td>\n<\/tr>\n<tr>\n<td width=\"177\">Thermo Scientific\u2122 <a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/37071?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">SuperSignal\u2122 West Dura<br \/>\nExtended Duration<br \/>\nSubstrate<\/a><\/td>\n<td width=\"75\">West Dura<\/td>\n<td width=\"71\">24\u6642\u9593<\/td>\n<td width=\"162\">\u30d5\u30a7\u30e0\u30c8\u30b0\u30e9\u30e0\u4e2d\u57df<\/td>\n<td width=\"169\">1\u00b0: 1:5,000<br \/>\n2\u00b0: 1:50,000\uff5e1:250,000<\/td>\n<\/tr>\n<tr>\n<td width=\"177\">Thermo Scientific\u2122 <a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/34579?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">SuperSignal\u2122 West Pico<br \/>\nPLUS Chemiluminescent<br \/>\nSubstrate<\/a><\/td>\n<td width=\"75\">West Pico PLUS<\/td>\n<td width=\"71\">\uff5e24\u6642\u9593<\/td>\n<td width=\"162\">\u30d4\u30b3\u30b0\u30e9\u30e0\u4f4e\u57df\uff5e\u30d5\u30a7\u30e0\u30c8\u30b0\u30e9\u30e0\u9ad8\u57df<\/td>\n<td width=\"169\">1\u00b0: 1:5,000<br \/>\n2\u00b0: 1:20,000\uff5e1:100,000<\/td>\n<\/tr>\n<tr>\n<td width=\"177\">Thermo Scientific\u2122<br \/>\n<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/32134?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">Pierce\u2122 ECL Plus Western Blotting Substrate<\/a><\/td>\n<td width=\"75\">Pierce ECL Plus<\/td>\n<td width=\"71\">5\u6642\u9593<\/td>\n<td width=\"162\">\u30d4\u30b3\u30b0\u30e9\u30e0\u4f4e\u57df\uff5e\u30d5\u30a7\u30e0\u30c8\u30b0\u30e9\u30e0\u4e2d\u57df<\/td>\n<td width=\"169\">1\u00b0: 1:1,000<br \/>\n2\u00b0: 1:25,000\uff5e1:200,000<\/td>\n<\/tr>\n<tr>\n<td width=\"177\">Thermo Scientific\u2122<br \/>\n<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/32109?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">Pierce\u2122 ECL Western Blotting Substrate<\/a><\/td>\n<td width=\"75\">Pierce ECL<\/td>\n<td width=\"71\">1\uff5e2\u6642\u9593<\/td>\n<td width=\"162\">\u30d4\u30b3\u30b0\u30e9\u30e0\u4f4e\u57df<\/td>\n<td width=\"169\">1\u00b0: 1:1,000<br \/>\n2\u00b0: 1:5,000\uff5e1:10,000<\/td>\n<\/tr>\n<\/tbody>\n<tfoot>\n<tr>\n<td colspan=\"4\"><span style=\"font-size: 12px\">\u203b\u6297\u4f531 mg\/mL\u3092\u7528\u3044\u305f\u5834\u5408\u306e\u5e0c\u91c8\u500d\u7387\u3067\u3059\u3002<\/span><\/td>\n<\/tr>\n<\/tfoot>\n<\/table>\n<table width=\"648\">\n<caption><b>\u88685. AP\u7528\u5316\u5b66\u767a\u5149\u691c\u51fa\u8a66\u85ac\uff08\u57fa\u8cea\uff09<\/b><\/caption>\n<tbody>\n<tr>\n<td width=\"200\"><b>\u767a\u8272\u7528\u691c\u51fa\u8a66\u85ac<\/b><\/td>\n<td width=\"85\"><b>\u691c\u51fa\u9650\u754c<\/b><\/td>\n<td width=\"161\"><b>\u63a8\u5968\u306e\u6297\u4f53\u5e0c\u91c8\u500d\u7387<sup>\u203b<\/sup><\/b><\/td>\n<td width=\"202\"><b>\u6ce8\u610f\u4e8b\u9805<\/b><\/td>\n<\/tr>\n<tr>\n<td width=\"200\">Invitrogen\u2122 <a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/WP20002?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">Novex\u2122 AP Chemiluminescent<br \/>\nSubstrate (CDP-Star\u2122)<\/a><\/td>\n<td width=\"85\">\u30d5\u30a7\u30e0\u30c8\u30b0\u30e9\u30e0\u4f4e\u57df\uff5e\u4e2d\u57df<\/td>\n<td width=\"161\">1\u00b0: 1:500\uff5e1:5,000<br \/>\n2\u00b0: 1:2,000\uff5e1:10,000<\/td>\n<td width=\"202\">\u30fbPVDF \u30e1\u30f3\u30d6\u30ec\u30f3\u7528<br \/>\n\u30fb\u30d0\u30c3\u30d5\u30a1\u30fc\u3068\u3057\u3066PBS\u306f\u4f7f\u7528\u3067\u304d\u306a\u3044<\/td>\n<\/tr>\n<tr>\n<td width=\"200\">Invitrogen\u2122 <a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/T2218?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">CDP-<em>Star<\/em>\u2122 Substrate with<br \/>\nNitro-Block-II\u2122 Enhancer<\/a><\/td>\n<td width=\"85\">\u30d5\u30a7\u30e0\u30c8\u30b0\u30e9\u30e0\u4f4e\u57df\uff5e\u4e2d\u57df<\/td>\n<td width=\"161\">1\u00b0: 1:500\uff5e1:5,000<br \/>\n2\u00b0: 1:2,000\uff5e1:10,000<\/td>\n<td width=\"202\">\u30fb\u30cb\u30c8\u30ed\u30bb\u30eb\u30ed\u30fc\u30b9\u30e1\u30f3\u30d6\u30ec\u30f3\u7528<br \/>\n\u30fb\u30d0\u30c3\u30d5\u30a1\u30fc\u3068\u3057\u3066PBS\u306f\u4f7f\u7528\u3067\u304d\u306a\u3044<\/td>\n<\/tr>\n<\/tbody>\n<tfoot>\n<tr>\n<td colspan=\"4\"><span style=\"font-size: 12px\">\u203b\u6297\u4f531 mg\/mL\u3092\u7528\u3044\u305f\u5834\u5408\u306e\u5e0c\u91c8\u500d\u7387\u3067\u3059\u3002<\/span><\/td>\n<\/tr>\n<\/tfoot>\n<\/table>\n<p>\u691c\u51fa\u8a66\u85ac\u306e\u611f\u5ea6\u306f\u88684\u30015\u306e\u3088\u3046\u306b\u8a66\u85ac\u306b\u3088\u3063\u3066\u7570\u306a\u308a\u307e\u3059\u304c\u3001\u611f\u5ea6\u306f\u9ad8\u3051\u308c\u3070\u9ad8\u3044\u307b\u3069\u3088\u3044\u3068\u3044\u3046\u308f\u3051\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u611f\u5ea6\u304c\u9ad8\u3044\u8a66\u85ac\u306e\u5834\u5408\u3001\u611f\u5ea6\u304c\u4f4e\u3044\u8a66\u85ac\u3068\u6bd4\u3079\u3066\u6761\u4ef6\u691c\u8a0e\u304c\u96e3\u3057\u304f\u306a\u308b\u304b\u3089\u3067\u3059\u3002\u305d\u306e\u305f\u3081\u3001\u4f8b\u3048\u3070\u901a\u5e38\u306e\u691c\u51fa\u306b\u306fPierce ECL Plus\u304b\u3089West Dura\u7a0b\u5ea6\u306e\u611f\u5ea6\u306e\u3082\u306e\u3092\u7528\u3044\u3066\u3001\u691c\u51fa\u3067\u304d\u306a\u3044\u3068\u304d\u306b\u3060\u3051\u3001\u3055\u3089\u306b\u9ad8\u611f\u5ea6\u306eWest Femto\u3084West Atto\u3092\u4f7f\u7528\u3059\u308b\u3068\u3044\u3063\u305f\u3088\u3046\u306b\u3001\u4f7f\u3044\u5206\u3051\u3092\u304a\u3059\u3059\u3081\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>\u307e\u305f\u3001\u691c\u51fa\u8a66\u85ac\u306e\u611f\u5ea6\u304c\u9ad8\u3051\u308c\u3070\u9ad8\u3044\u307b\u3069\u3001\u63a8\u5968\u306e\u6297\u4f53\u306e\u5e0c\u91c8\u500d\u7387\u304c\u9ad8\u304f\uff08\u3064\u307e\u308a\u3001\u6297\u4f53\u6fc3\u5ea6\u304c\u8584\u304f\uff09\u306a\u308b\u70b9\u306b\u3082\u6ce8\u610f\u3057\u307e\u3057\u3087\u3046\u3002\u3064\u307e\u308a\u3001\u691c\u51fa\u8a66\u85ac\u3092\u4f4e\u3044\u3082\u306e\u304b\u3089\u9ad8\u3044\u3082\u306e\u306b\u5909\u66f4\u3057\u305f\u5834\u5408\u3001\u6297\u4f53\u5e0c\u91c8\u6fc3\u5ea6\u304c\u305d\u306e\u307e\u307e\u3060\u3068\u3001\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9\u304c\u4e0a\u304c\u308b\u306a\u3069\u3001\u30c8\u30e9\u30d6\u30eb\u306e\u539f\u56e0\u306b\u306a\u308a\u307e\u3059\u3002\u6297\u4f53\u306f\u3001\u691c\u51fa\u8a66\u85ac\u306e\u611f\u5ea6\u306b\u5408\u308f\u305b\u3066\u5e0c\u91c8\u3057\u3066\u304f\u3060\u3055\u3044\uff08\u6297\u4f53\u306e\u5e0c\u91c8\u500d\u7387\u306b\u3064\u3044\u3066\u306f\u3001<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/western-blot-protein-ts-6\/#i-6\" target=\"_blank\" rel=\"noopener\">\u3053\u3061\u3089<\/a>\u3082\u53c2\u8003\u306b\u3057\u3066\u304f\u3060\u3055\u3044\uff09\u3002\u8cb4\u91cd\u306a\u6297\u4f53\u306e\u5834\u5408\u306f\u6297\u4f53\u91cf\u3092\u5c11\u306a\u304f\u3067\u304d\u308b\u30e1\u30ea\u30c3\u30c8\u3082\u3042\u308a\u307e\u3059\u3002<\/p>\n<p>\u6700\u5f8c\u306b\u3001\u5316\u5b66\u767a\u5149\u306e\u691c\u51fa\u65b9\u6cd5\u306b\u3064\u3044\u3066\u3067\u3059\u3002<br \/>\n\u5316\u5b66\u767a\u5149\u306e\u691c\u51fa\u306b\u306f\u3001CCD\u30ab\u30e1\u30e9\u3092\u642d\u8f09\u3057\u305f\u30a4\u30e1\u30fc\u30b8\u30e3\u30fc\u3084X\u7dda\u30d5\u30a3\u30eb\u30e0\u3067\u306e\u691c\u51fa\u304c\u5fc5\u8981\u306b\u306a\u308a\u307e\u3059\u3002\u73fe\u5728\u306f\u3001X\u7dda\u30d5\u30a3\u30eb\u30e0\u3088\u308a\u3082\u3001\u30c7\u30b8\u30bf\u30eb\u30c7\u30fc\u30bf\u3068\u3057\u3066\u753b\u50cf\u304c\u5165\u624b\u3067\u304d\u308bCCD\u30ab\u30e1\u30e9\u304c\u4e3b\u6d41\u3067\u3059\u3002\u307e\u305f\u3001CCD\u30ab\u30e1\u30e9\u3067\u3042\u308c\u3070\u3001\u9732\u5149\u6642\u9593\u306e\u8abf\u6574\u3082\u5bb9\u6613\u3067\u3059\u3002\u9732\u5149\u3068\u306f\u3001X\u7dda\u30d5\u30a3\u30eb\u30e0\u3084CCD\u306a\u3069\u306e\u64ae\u5f71\u7d20\u5b50\u306b\u5149\u3092\u5f53\u3066\u308b\u3053\u3068\u3092\u8a00\u3044\u307e\u3059\u304c\u3001\u5404\u30e1\u30f3\u30d6\u30ec\u30f3\u306b\u6700\u9069\u306a\u9732\u5149\u6642\u9593\u3092X\u7dda\u30d5\u30a3\u30eb\u30e0\u3067\u63a2\u3057\u51fa\u3059\u306e\u306f\u3001\u624b\u4f5c\u696d\u3067\u73fe\u50cf\u3092\u7e70\u308a\u8fd4\u305b\u306d\u3070\u306a\u3089\u305a\u3001\u3069\u3046\u3057\u3066\u3082\u624b\u9593\u304c\u304b\u304b\u308a\u307e\u3059\u3002<\/p>\n<p>\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u691c\u51fa\u7528\u306eInvitrogen\u2122 <a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/protein-biology\/protein-assays-analysis\/western-blotting\/detect-proteins-western-blot\/western-blot-imaging-analysis\/ibright-systems.html?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">iBright\u2122<\/a>\u30b7\u30ea\u30fc\u30ba\u306f Smart Exposure\u2122\u30c6\u30af\u30ce\u30ed\u30b8\u30fc\u306b\u3088\u308a\u3001\u6700\u9069\u306a\u9732\u5149\u6642\u9593\u3092\u6a5f\u5668\u304c\u7b97\u51fa\u3057\u3066\u304f\u308c\u308b\u3060\u3051\u3067\u306a\u304f\u3001\u9732\u5149\u6642\u9593\u306b\u5fdc\u3058\u305f\u4eee\u60f3\u753b\u50cf\u3092\u8868\u793a\u3067\u304d\u308b\u305f\u3081\u3001\u7684\u78ba\u306a\u9732\u5149\u6642\u9593\u304c\u8a2d\u5b9a\u3057\u3084\u3059\u304f\u3001\u904e\u5270\u9732\u5149\u3084\u9732\u5149\u4e0d\u8db3\u3092\u9632\u3050\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u6838\u9178\u306e\u691c\u51fa\u3084\u86cd\u5149\u691c\u51fa\u203b\u3082\u53ef\u80fd\u3067\u3001\u7121\u6599\u306e\u89e3\u6790\u30bd\u30d5\u30c8\u30a6\u30a8\u30a2\u304c\u4ed8\u5c5e\u3057\u3066\u3044\u307e\u3059\u3002\u30e6\u30fc\u30b6\u30fc\u30a4\u30f3\u30bf\u30fc\u30d5\u30a7\u30fc\u30b9\u304c\u9ad8\u304f\u3001\u611f\u899a\u7684\u306b\u30dc\u30bf\u30f3\u3092\u30bf\u30c3\u30d7\u3057\u3066\u3044\u304f\u3060\u3051\u3067\u624b\u8efd\u306b\u624b\u65e9\u304f\u3001\u7684\u78ba\u306a\u753b\u50cf\u64ae\u5f71\u304c\u3067\u304d\u308b\u70b9\u304c\u3053\u306e\u88c5\u7f6e\u306e\u5927\u304d\u306a\u30e1\u30ea\u30c3\u30c8\u3067\u3059\u3002<br \/>\n<span style=\"font-size: 12px\">\u203b\u6a5f\u7a2e\u306b\u3088\u308a\u307e\u3059\u3002<\/span><\/p>\n<div id=\"attachment_15610\" style=\"width: 792px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-15610\" class=\"size-full wp-image-15610\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-02.jpg\" alt=\"West Dura\u3067\u691c\u51fa\u3057\u305f\u7d50\u679c\" width=\"782\" height=\"146\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-02.jpg 782w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-02-300x56.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-02-768x143.jpg 768w\" sizes=\"auto, (max-width: 782px) 100vw, 782px\" \/><p id=\"caption-attachment-15610\" class=\"wp-caption-text\">\u56f32. <a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/37071?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">West Dura<\/a>\u3067\u691c\u51fa\u3057\u305f\u7d50\u679c\u3000\uff08\u5de6\uff1a\u53cd\u5fdc\u7d42\u4e86\u5f8c1\u5206\u5f8c\u306e\u691c\u51fa\u7d50\u679c\u3001\u53f3\uff1a\u53cd\u5fdc\u7d42\u4e86\u5f8c4.5\u6642\u9593\u5f8c\u306e\u691c\u51fa\u7d50\u679c\uff09<br \/>\u30b5\u30f3\u30d7\u30eb\uff1aA431 Lysate\u3001\u4e00\u6b21\u6297\u4f53\uff1a\u6297\u03b2-\u30ab\u30c6\u30cb\u30f3\uff08\u88fd\u54c1\u756a\u53f7 MA1-300)<\/p><\/div>\n<h2>\u86cd\u5149\u691c\u51fa<\/h2>\n<p>\u9577\u5e74\u3001\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u4e3b\u6d41\u306f\u5316\u5b66\u767a\u5149\u691c\u51fa\u3067\u3057\u305f\u304c\u3001\u86cd\u5149\u306b\u95a2\u3059\u308b\u6280\u8853\u304c\u6210\u719f\u3057\u3001\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u3067\u86cd\u5149\u691c\u51fa\u3092\u3059\u308b\u7814\u7a76\u8005\u306f\u5897\u52a0\u306e\u50be\u5411\u306b\u3042\u308a\u307e\u3059\u3002\u86cd\u5149\u691c\u51fa\u306f\u3001\u540c\u4e00\u30e1\u30f3\u30d6\u30ec\u30f3\u5185\u3067\u8907\u6570\u306e\u6297\u4f53\u3092\u7528\u3044\u3066\u591a\u91cd\u691c\u51fa\u304c\u5bb9\u6613\u3068\u3044\u3046\u5927\u304d\u306a\u30e1\u30ea\u30c3\u30c8\u304c\u3042\u308a\u307e\u3059\uff08\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u691c\u51fa\uff09\u3002\u3064\u307e\u308a\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u30ed\u30fc\u30c7\u30a3\u30f3\u30b0\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u306e\u540c\u6642\u691c\u51fa\u3084\u3001\u5206\u5b50\u91cf\u304c\u8fd1\u3044\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u8b58\u5225\u3001\u30ea\u30f3\u9178\u5316\u30bf\u30f3\u30d1\u30af\u8cea\u306a\u3069\u306e\u691c\u51fa\u304c\u5bb9\u6613\u306b\u3067\u304d\u308b\u306e\u3067\u3059\uff08\u56f33\u3001\u56f34\uff09\u3002\u3055\u3089\u306b\u3001\u5316\u5b66\u767a\u5149\u3088\u308a\u3082\u86cd\u5149\u306e\u65b9\u304c\u30c0\u30a4\u30ca\u30df\u30c3\u30af\u30ec\u30f3\u30b8\u304c\u5e83\u304f\u3001\u5b9a\u91cf\u6027\u304c\u9ad8\u3044\u3068\u3044\u3046\u5229\u70b9\u3082\u3042\u308a\u307e\u3059\u3002<\/p>\n<div id=\"attachment_15611\" style=\"width: 638px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-15611\" class=\"size-full wp-image-15611\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-03.jpg\" alt=\"\u30bf\u30fc\u30b2\u30c3\u30c8\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u30ed\u30fc\u30c7\u30a3\u30f3\u30b0\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u540c\u6642\u691c\u51fa\" width=\"628\" height=\"210\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-03.jpg 628w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-03-300x100.jpg 300w\" sizes=\"auto, (max-width: 628px) 100vw, 628px\" \/><p id=\"caption-attachment-15611\" class=\"wp-caption-text\">\u56f33. \u30bf\u30fc\u30b2\u30c3\u30c8\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u30ed\u30fc\u30c7\u30a3\u30f3\u30b0\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u540c\u6642\u691c\u51fa<\/p><\/div>\n<div id=\"attachment_15612\" style=\"width: 726px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-15612\" class=\"size-full wp-image-15612\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-04.jpg\" alt=\"\u5206\u5b50\u91cf\u304c\u8fd1\u63a5\u3057\u305f\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u691c\u51fa\" width=\"716\" height=\"262\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-04.jpg 716w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-04-300x110.jpg 300w\" sizes=\"auto, (max-width: 716px) 100vw, 716px\" \/><p id=\"caption-attachment-15612\" class=\"wp-caption-text\">\u56f34. \u5206\u5b50\u91cf\u304c\u8fd1\u63a5\u3057\u305f\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u691c\u51fa<\/p><\/div>\n<p>\u3067\u306f\u3001\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u86cd\u5149\u691c\u51fa\u6642\u306e\u9078\u629e\u306e\u30dd\u30a4\u30f3\u30c8\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n<p>\u25c6\u30b5\u30f3\u30d7\u30eb\u30d0\u30c3\u30d5\u30a1\u30fc<br \/>\n\u3088\u304f\u5229\u7528\u3055\u308c\u308bBPB\uff08bromophenol blue\uff09\u306f\u86cd\u5149\u3092\u767a\u3059\u308b\u305f\u3081\u3001\u30c8\u30e9\u30c3\u30ad\u30f3\u30b0\u30c0\u30a4\u3068\u3057\u3066\u4f7f\u7528\u3059\u308b\u3068\u3001\u30b2\u30eb\u30dc\u30c8\u30e0\u4ed8\u8fd1\u3067\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9\u304c\u751f\u3058\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u30b5\u30f3\u30d7\u30eb\u30d0\u30c3\u30d5\u30a1\u30fc\u306b\u86cd\u5149\u6210\u5206\u3092\u542b\u307e\u306a\u3044Invitrogen\u2122 <a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/LC2570?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">Fluorescent Compatible Sample Buffer<\/a>\u304c\u304a\u3059\u3059\u3081\u3067\u3059\u3002<\/p>\n<p>\u25c6\u30e1\u30f3\u30d6\u30ec\u30f3<br \/>\nPVDF\u30e1\u30f3\u30d6\u30ec\u30f3\u306f\u81ea\u5bb6\u86cd\u5149\u3092\u6301\u3063\u3066\u3044\u307e\u3059\u306e\u3067\u3001\u86cd\u5149\u691c\u51fa\u306e\u3068\u304d\u306f<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/22860?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u4f4e\u86cd\u5149PVDF\u30e1\u30f3\u30d6\u30ec\u30f3<\/a>\u304b\u3001\u30cb\u30c8\u30ed\u30bb\u30eb\u30ed\u30fc\u30b9\u30e1\u30f3\u30d6\u30ec\u30f3\u3092\u4f7f\u7528\u3057\u307e\u3057\u3087\u3046\u3002<\/p>\n<p>\u25c6\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u30d0\u30c3\u30d5\u30a1\u30fc<br \/>\n\u5fae\u7c92\u5b50\u3092\u542b\u307e\u306a\u3044\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u30d0\u30c3\u30d5\u30a1\u30fc\u3092\u7528\u3044\u307e\u3059\u3002\u5fae\u7c92\u5b50\u304c\u30e1\u30f3\u30d6\u30ec\u30f3\u306b\u4ed8\u7740\u3057\u3001\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9\u306e\u539f\u56e0\u306b\u306a\u308b\u305f\u3081\u3067\u3059\u3002\u4f8b\u3048\u3070\u3001\u86cd\u5149\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u7528\u306b\u958b\u767a\u3055\u308c\u305fThermo Scientific\u2122 <a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/37565?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">Blocker\u2122 FL Fluorescent Blocking Buffer (10X)<\/a>\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<p>\u25c6\u5206\u5b50\u91cf\u30de\u30fc\u30ab\u30fc<br \/>\n\u7740\u8272\u6e08\u306e\u5206\u5b50\u91cf\u30de\u30fc\u30ab\u30fc\u306f\u3001\u4f7f\u7528\u3055\u308c\u3066\u3044\u308b\u8272\u7d20\u306b\u3088\u3063\u3066\u3001\u7279\u5b9a\u306e\u6ce2\u9577\u306e\u86cd\u5149\u3092\u793a\u3057\u3001\u86cd\u5149\u691c\u51fa\u7528\u306e\u5206\u5b50\u91cf\u30de\u30fc\u30ab\u30fc\u3068\u3057\u3066\u4f7f\u7528\u3067\u304d\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\uff08\u88686\uff09\u3002<\/p>\n<table>\n<caption><b>\u88686. \u86cd\u5149\u691c\u51fa\u306b\u7528\u3044\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u7740\u8272\u6e08\u5206\u5b50\u91cf\u30de\u30fc\u30ab\u30fc<\/b><\/caption>\n<tbody>\n<tr>\n<td width=\"327\"><b>\u5206\u5b50\u91cf\u30de\u30fc\u30ab\u30fc<\/b><\/td>\n<td width=\"327\"><b>\u6cf3\u52d5\u50cf\u3068\u3001iBright FL1500 System\u3067\u306e\u691c\u51fa\u6761\u4ef6<\/b><br \/>\n\u203b\u30af\u30ea\u30c3\u30af\u3059\u308b\u3068\u62e1\u5927\u3057\u307e\u3059<\/td>\n<\/tr>\n<tr>\n<td width=\"327\">Thermo Scientific\u2122<br \/>\n<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/26634?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">Spectra\u2122 Multicolor Broad Range Protein Ladder<\/a><\/td>\n<td width=\"327\"><a href=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-05.jpg\" target=\"_blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-15618\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-05-150x150.jpg\" alt=\"\" width=\"98\" height=\"130\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-05-227x300.jpg 227w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-05.jpg 243w\" sizes=\"auto, (max-width: 98px) 100vw, 98px\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td width=\"327\">Thermo Scientific\u2122<br \/>\n<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/26616?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">PageRuler\u2122 Prestained Protein Ladder<\/a><\/td>\n<td width=\"327\"><a href=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-06.jpg\" target=\"_blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-15619\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-06.jpg\" alt=\"\" width=\"94\" height=\"130\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-06.jpg 231w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-06-217x300.jpg 217w\" sizes=\"auto, (max-width: 94px) 100vw, 94px\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td width=\"327\">Thermo Scientific\u2122<br \/>\n<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/26619?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">PageRuler\u2122 Plus Prestained Protein Ladder<\/a><\/td>\n<td width=\"327\"><a href=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-07.jpg\" target=\"_blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-15613\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-07.jpg\" alt=\"\" width=\"99\" height=\"130\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-07.jpg 242w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-07-228x300.jpg 228w\" sizes=\"auto, (max-width: 99px) 100vw, 99px\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td width=\"327\">Invitrogen\u2122<br \/>\n<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/LC5615?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">iBright\u2122 Prestained Protein Ladder<\/a><\/td>\n<td width=\"327\"><a href=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-08.jpg\" target=\"_blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-15614\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-08.jpg\" alt=\"\" width=\"100\" height=\"130\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-08.jpg 248w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-08-232x300.jpg 232w\" sizes=\"auto, (max-width: 100px) 100vw, 100px\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td width=\"327\">Invitrogen\u2122<br \/>\n<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/LC5699?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">HiMark\u2122 Pre-stained Protein Standard<\/a><\/td>\n<td width=\"327\"><a href=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-09.jpg\" target=\"_blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-15615\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-09.jpg\" alt=\"\" width=\"77\" height=\"130\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-09.jpg 191w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-09-179x300.jpg 179w\" sizes=\"auto, (max-width: 77px) 100vw, 77px\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td width=\"327\">Thermo Scientific\u2122<br \/>\n<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/26625?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">Spectra\u2122 Multicolor High Range Protein Ladder<\/a><\/td>\n<td width=\"327\"><a href=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-10.jpg\" target=\"_blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-15616\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-10.jpg\" alt=\"\" width=\"98\" height=\"130\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-10.jpg 243w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-10-227x300.jpg 227w\" sizes=\"auto, (max-width: 98px) 100vw, 98px\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td width=\"327\">Thermo Scientific\u2122<br \/>\n<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/26628?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">Spectra\u2122 Multicolor Low Range Protein Ladder<\/a><\/td>\n<td width=\"327\"><a href=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-11.jpg\" target=\"_blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-15617\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-11.jpg\" alt=\"\" width=\"93\" height=\"130\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-11.jpg 227w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-11-213x300.jpg 213w\" sizes=\"auto, (max-width: 93px) 100vw, 93px\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>\u25c6\u6297\u4f53<br \/>\n\u86cd\u5149\u691c\u51fa\u6642\u306f\u3001\u86cd\u5149\u6a19\u8b58\u4e8c\u6b21\u6297\u4f53\u3092\u7528\u3044\u308b\u3053\u3068\u304c\u591a\u3044\u3067\u3059\u3002<br \/>\n\u9078\u51fa\u6642\u306f\u3001\u6b21\u306e\u3088\u3046\u306a\u70b9\u306b\u6ce8\u610f\u3057\u307e\u3057\u3087\u3046\u3002<\/p>\n<ul>\n<li>\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u7528\u306e\u6297\u4f53\u3092\u7528\u3044\u308b\uff08<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/western-blot-protein-ts-6\/?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u7b2c6\u56de<\/a>\u3082\u53c2\u8003\u306b\u3057\u3066\u304f\u3060\u3055\u3044\uff09<\/li>\n<li>\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u691c\u51fa\u306e\u5834\u5408\u3001\u5404\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u4e00\u6b21\u6297\u4f53\u306f\u30db\u30b9\u30c8\u306e\u751f\u7269\u7a2e\u304c\u7570\u306a\u308b\u3088\u3046\u306b\u9078\u3076<br \/>\n\u203b\u53ef\u80fd\u306a\u5834\u5408\u306f\u3001\u3055\u3089\u306b\u30db\u30b9\u30c8\u751f\u7269\u7a2e\u3068\u3057\u3066\u306a\u308b\u3079\u304f\u96e2\u308c\u305f\u7d44\u307f\u5408\u308f\u305b\u306b\u3059\u308b\u3068\u3001\u4ea4\u5dee\u53cd\u5fdc\u3092\u6291\u3048\u3084\u3059\u3044<br \/>\n\uff08\u4f8b\u3048\u3070\u30de\u30a6\u30b9\u3068\u30e9\u30c3\u30c8\u3001\u30e4\u30ae\u3068\u30d2\u30c4\u30b8\u306e\u7d44\u307f\u5408\u308f\u305b\u3088\u308a\u306f\u3001\u30e9\u30c3\u30c8\u3068\u30e9\u30d3\u30c3\u30c8\u306b\u3059\u308b\u306a\u3069\uff09<\/li>\n<li>\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u691c\u51fa\u306e\u5834\u5408\u3001\u4e8c\u6b21\u6297\u4f53\u306b\u6a19\u8b58\u3055\u308c\u305f\u86cd\u5149\u8272\u7d20\u306f\u30af\u30ed\u30b9\u30c8\u30fc\u30af\u304c\u6291\u3048\u3089\u308c\u308b\u7d44\u307f\u5408\u308f\u305b\u3092\u9078\u3076<\/li>\n<\/ul>\n<p>\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u3067\u306e\u86cd\u5149\u8272\u7d20\u306f\u3001\u30e9\u30a4\u30f3\u30a2\u30c3\u30d7\u304c\u8c4a\u5bcc\u306aInvitrogen\u2122 <a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/brands\/molecular-probes\/key-molecular-probes-products\/alexa-fluor.html?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">Alexa Fluor\u2122<\/a>\u6297\u4f53\u304c\u304a\u3059\u3059\u3081\u3067\u3059\u3002\u305d\u308c\u305e\u308c\u306e\u86cd\u5149\u8272\u7d20\u306e\u52b1\u8d77\u30fb\u86cd\u5149\u6ce2\u9577\u3068\u3001\u86cd\u5149\u691c\u51fa\u6a5f\u5668\u306e\u30d5\u30a3\u30eb\u30bf\u30fc\u6ce2\u9577\u3092\u78ba\u8a8d\u3057\u3066\u9078\u629e\u3057\u307e\u3057\u3087\u3046\u3002<\/p>\n<p>\u4f8b\u3048\u3070Invitrogen\u2122 <a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/protein-biology\/protein-assays-analysis\/western-blotting\/detect-proteins-western-blot\/western-blot-imaging-analysis\/ibright-systems.html?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">iBright FL1500 Imaging System<\/a>\u3067\u306f\u3001\u52b1\u8d77\u30d5\u30a3\u30eb\u30bf\u30fc\u3068\u86cd\u5149\u30d5\u30a3\u30eb\u30bf\u30fc\u304c5\u7a2e\u985e\uff08X1\uff5e5\u3001M1\uff5e5\uff09\u642d\u8f09\u3055\u308c\u3066\u304a\u308a\uff08\u88687\uff09\u3001\u6700\u59274\u7a2e\u985e\u307e\u3067\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u691c\u51fa\u304c\u53ef\u80fd\u3067\u3059\uff08\u88688\uff09\u3002<\/p>\n<p style=\"text-align: center\"><b>\u88687. iBright FL1500 System\u306e\u52b1\u8d77\u30fb\u86cd\u5149\u30d5\u30a3\u30eb\u30bf\u30fc\u3068Invitrogen\u2122 Alexa Fluor\u2122\u86cd\u5149\u8272\u7d20\u306e\u4f8b<\/b><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-15620 aligncenter\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-12.jpg\" alt=\"iBright FL1500 System\u306e\u52b1\u8d77\u30fb\u86cd\u5149\u30d5\u30a3\u30eb\u30bf\u30fc\u3068Invitrogen\u2122 Alexa Fluor\u2122\u86cd\u5149\u8272\u7d20\u306e\u4f8b\" width=\"642\" height=\"337\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-12.jpg 642w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-12-300x157.jpg 300w\" sizes=\"auto, (max-width: 642px) 100vw, 642px\" \/><\/p>\n<p>X3\uff0fM3\u304a\u3088\u3073X4\uff0fM4\u30c1\u30e3\u30cd\u30eb\u306e\u86cd\u5149\u8272\u7d20\u306f\u30b9\u30da\u30af\u30c8\u30eb\u306e\u30aa\u30fc\u30d0\u30fc\u30e9\u30c3\u30d7\u304c\u5927\u304d\u3044\u305f\u3081\u3001\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u691c\u51fa\u3067\u306f\u3053\u308c\u3089\u306e\u30c1\u30e3\u30cd\u30eb\u3092\u540c\u6642\u306b\u4f7f\u308f\u306a\u3044\u3088\u3046\u306b\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n<p style=\"text-align: center\"><b>\u88688. iBright FL1500 System\u3067\u306eAlexa Fluor\u86cd\u5149\u8272\u7d20\u306b\u3088\u308b\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u7d44\u307f\u5408\u308f\u305b\u4f8b<br \/>\n<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-15621\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-13.jpg\" alt=\"\" width=\"637\" height=\"257\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-13.jpg 637w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/10\/western-blot-protein-ts-7-13-300x121.jpg 300w\" sizes=\"auto, (max-width: 637px) 100vw, 637px\" \/><\/b><\/p>\n<p>\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u691c\u51fa\u306b\u3064\u3044\u3066\u306f\u3001\u4e0b\u8a18\u30ea\u30f3\u30af\u3082\u53c2\u8003\u306b\u3057\u3066\u304f\u3060\u3055\u3044\u3002<br \/>\n\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u30ce\u30fc\u30c8\uff1a<a href=\"https:\/\/assets.thermofisher.com\/TFS-Assets\/BID\/Technical-Notes\/fluorescent-western-blotting-guide-multiplexing-tech-note.pdf?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">Fluorescent western blotting-a guide to multiplexing<\/a><br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/westernblotting_ibright_bid_ts_1\/?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">Multiplex\u86cd\u5149\u30a6\u30a7\u30b9\u30bf\u30f3\u691c\u51fa\u3067\u5931\u6557\u3057\u306a\u3044\u305f\u3081\u306b<\/a><\/p>\n<h2>\u307e\u3068\u3081<\/h2>\n<p>\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306b\u304a\u3051\u308b\u691c\u51fa\u8a66\u85ac\u306b\u306f\u3001\u767a\u8272\u30fb\u5316\u5b66\u767a\u5149\u30fb\u86cd\u5149\u306e\u4e3b\u306b3\u7a2e\u985e\u304c\u3042\u308a\u307e\u3059\u3002\u305d\u308c\u305e\u308c\u30e1\u30ea\u30c3\u30c8\u3068\u30c7\u30e1\u30ea\u30c3\u30c8\u304c\u3042\u308a\u3001\u88fd\u54c1\u3082\u591a\u5c90\u306b\u308f\u305f\u308b\u305f\u3081\u3001\u304a\u6301\u3061\u306e\u6a5f\u5668\u3084\u5b9f\u9a13\u76ee\u7684\u306b\u5408\u308f\u305b\u3066\u9078\u3073\u307e\u3057\u3087\u3046\u3002<\/p>\n<p>\u6b21\u56de\u306f\u3001\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u30b9\u30c8\u30ea\u30c3\u30d4\u30f3\u30b0\u30fb\u30ea\u30d7\u30ed\u30fc\u30d3\u30f3\u30b0\u306b\u3064\u3044\u3066\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n<p>\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u9078\u3073\u65b9\u30b7\u30ea\u30fc\u30ba\u306f\u3053\u3061\u3089\uff1a<br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/western-blot-protein-ts-1\/?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u88fd\u54c1\u9078\u3073\u306b\u3082\u3046\u56f0\u3089\u306a\u3044\uff01\u7b2c1\u56de\uff1a\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u62bd\u51fa\u65b9\u6cd5<\/a><br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/western-blot-protein-ts-2\/?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u88fd\u54c1\u9078\u3073\u306b\u3082\u3046\u56f0\u3089\u306a\u3044\uff01\u7b2c2\u56de\uff1a\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u30ad\u30c3\u30c8<\/a><br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/western-blot-protein-ts-3\/?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">SDS-PAGE\u306e\u9078\u3073\u65b9\uff1a\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u88fd\u54c1\u9078\u3073\u306b\u3082\u3046\u56f0\u3089\u306a\u3044\uff01\u7b2c3\u56de<\/a><br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/western-blot-protein-ts-4\/?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u8ee2\u5199\u6761\u4ef6\u306e\u9078\u3073\u65b9\uff1a\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u88fd\u54c1\u9078\u3073\u306b\u3082\u3046\u56f0\u3089\u306a\u3044\uff01\u7b2c4\u56de<\/a><br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/western-blot-protein-ts-5\/?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u8a66\u85ac\u306e\u30dd\u30a4\u30f3\u30c8\uff1a\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u88fd\u54c1\u9078\u3073\u306b\u3082\u3046\u56f0\u3089\u306a\u3044\uff01\u7b2c5\u56de<\/a><br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/western-blot-protein-ts-6\/?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u6297\u4f53\u3092\u9078\u3076\u3068\u304d\u306b\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\u3053\u3068\uff1a\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u88fd\u54c1\u9078\u3073\u7b2c6\u56de<\/a><br \/>\n\u5316\u5b66\u767a\u5149\u3068\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u691c\u51fa\u306e\u9078\u629e\u30dd\u30a4\u30f3\u30c8\uff1a\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u9078\u3073\u65b9\u3000\u7b2c7\u56de<\/p>\n<h3>\u3010\u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3011\u30bf\u30f3\u30d1\u30af\u8cea\u89e3\u6790\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u30cf\u30f3\u30c9\u30d6\u30c3\u30af<\/h3>\n<p>\u52b9\u7387\u7684\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u62bd\u51fa\u304b\u3089\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u89e3\u6790\u30c4\u30fc\u30eb\u307e\u3067\u3001\u5305\u62ec\u7684\u306b\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u3092\u7d39\u4ecb\u3057\u3066\u304a\u308a\u307e\u3059\u3002PDF\u30d5\u30a1\u30a4\u30eb\u306e\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3092\u3054\u5e0c\u671b\u306e\u65b9\u306f\u3001\u4e0b\u8a18\u30dc\u30bf\u30f3\u3088\u308a\u304a\u7533\u3057\u8fbc\u307f\u304f\u3060\u3055\u3044\u3002<\/p>\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/catalogs-protein-form.html?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western_blot_protein_ts_7_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/p>\n<p>&nbsp;<\/p>\n<p>\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u7528\u306b\u306f\u4f7f\u7528\u3044\u305f\u3060\u3051\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u300c\u9078\u3073\u65b9\u300d\u306b\u3064\u3044\u3066\u9023\u8f09\u3057\u3066\u3044\u308b\u672c\u30b7\u30ea\u30fc\u30ba\u3001\u524d\u56de\u306f\u6297\u4f53\u306b\u3064\u3044\u3066\u3054\u7d39\u4ecb\u3057\u307e\u3057\u305f\u3002\u6b21\u306f\u3044\u3088\u3044\u3088\u691c\u51fa\u306e\u30b9\u30c6\u30c3\u30d7\u3067\u3059\u3002\u9577\u3044\u5de5\u7a0b\u3092\u7d4c\u3066\u3001\u3088\u3046\u3084\u304f\u30d0\u30f3\u30c9\u304c\u78ba\u8a8d\u3067\u304d\u307e\u3059\u3002 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