{"id":15694,"date":"2023-11-10T22:43:26","date_gmt":"2023-11-10T13:43:26","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/?p=15694"},"modified":"2025-12-19T16:42:25","modified_gmt":"2025-12-19T07:42:25","slug":"pcr-fragment-workshop-gsd-ts-1","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/pcr-fragment-workshop-gsd-ts-1\/","title":{"rendered":"\u3010\u3084\u3063\u3066\u307f\u305f\u3011\u30a2\u30c7\u30cb\u30f3\u4ed8\u52a0\u53cd\u5fdc\u306ePCR\u8a66\u85ac\u306b\u3088\u308b\u9055\u3044\u3092\u8abf\u3079\u3066\u307f\u305f \uff5e\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u89e3\u6790\u3067\u60f3\u5b9a\u5916\u306e1\u5869\u57fa\u9055\u3044\u306e\u30d4\u30fc\u30af\u304c\u307f\u3089\u308c\u305f\u3089\u2026\u305d\u308c\u306f\u3001\u5897\u5e45\u3055\u308c\u305fPCR\u7523\u7269\u3078\u306e\u30a2\u30c7\u30cb\u30f3\u4ed8\u52a0\u304c\u539f\u56e0\u304b\u3082\u3057\u308c\u307e\u305b\u3093\uff5e"},"content":{"rendered":"<p>\u30b8\u30a7\u30cd\u30c6\u30a3\u30c3\u30af\u30a2\u30ca\u30e9\u30a4\u30b6\u306e\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306e1\u3064\u3067\u3042\u308b<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/ce_basic_dna_gsd_ts_3\/?CID=gsd_cap_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_og_s00_pcr_fragment_workshop_gsd_ts_1_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u89e3\u6790<\/a>\u3067\u306f\u3001PCR\u306a\u3069\u306b\u3088\u308a\u5f97\u3089\u308c\u305f\u86cd\u5149\u6a19\u8b58DNA\u65ad\u7247\u306e\u9577\u3055\u3092\u89e3\u6790\u3059\u308b\u3053\u3068\u3067\u3001\u9593\u63a5\u7684\u306b\u5869\u57fa\u914d\u5217\u306b\u95a2\u3059\u308b\u60c5\u5831\u3092\u77e5\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u4f8b\u3048\u3070\u3001\u300c\u30de\u30a4\u30af\u30ed\u30b5\u30c6\u30e9\u30a4\u30c8\u89e3\u6790\u300d\u3067\u306f\u3001\u7279\u5b9a\u5869\u57fa\u306e\u7e70\u308a\u8fd4\u3057\u6570\u306b\u6ce8\u76ee\u3057\u305f\u89e3\u6790\u3092\u3057\u307e\u3059\u3002\u5177\u4f53\u4f8b\u3068\u3057\u3066\u306f\u3001\u7e70\u308a\u8fd4\u3057\u9818\u57df\u306e\u4e2d\u3067\u3001\u500b\u4f53\u9593\u3067\u7570\u306a\u308b\u7e70\u308a\u8fd4\u3057\u6570\u306f\u3001\u89aa\u5b50\u5224\u5b9a\u3084\u500b\u4f53\u8b58\u5225\u306b\u6709\u52b9\u306aDNA\u30de\u30fc\u30ab\u30fc\u3068\u3057\u3066\u5229\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u3002<br \/>\n\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u89e3\u6790\u306e\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u4f8b\u3092\u56f31\u306b\u793a\u3057\u307e\u3059\u3002\u307e\u305a\u3001\u86cd\u5149\u6a19\u8b58\u3055\u308c\u305f\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u7528\u3044\u3066\u76ee\u7684\u9818\u57df\u3092PCR\u5897\u5e45\u3057\u3001\u5f97\u3089\u308c\u305fPCR\u7523\u7269\u3068\u30b5\u30a4\u30ba\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\uff08\u9577\u3055\u65e2\u77e5\u306e\u86cd\u5149\u6a19\u8b58DNA\u65ad\u7247\uff09\u3092\u6df7\u5408\u3057\u3001\u96fb\u6c17\u6cf3\u52d5\u3092\u884c\u3044\u307e\u3059\u3002\u30b5\u30a4\u30ba\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\u306e\u30d4\u30fc\u30af\u304b\u3089\u4f5c\u6210\u3057\u305f\u691c\u91cf\u7dda\u3092\u7528\u3044\u308b\u3053\u3068\u3067\u3001\u5bfe\u8c61\u30d4\u30fc\u30af\u306e\u30b5\u30a4\u30ba\u5024\u3092\u7b97\u51fa\u3057\u307e\u3059\u3002\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u89e3\u6790\u3067\u306f\u3001\u6a19\u8b58\u3055\u308c\u305f\u86cd\u5149\u8272\u7d20\u306e\u7a2e\u985e\u3084PCR\u7523\u7269\u306e\u9577\u3055\u304c\u7570\u306a\u308c\u3070\u3001\u8907\u6570\u306ePCR\u7523\u7269\u3092\u6df7\u5408\u3057\u3066\u6cf3\u52d5\u3067\u304d\u307e\u3059\u3002<\/p>\n<div id=\"attachment_15696\" style=\"width: 770px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-15696\" class=\"size-large wp-image-15696\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/11\/55bdb9631d844e9feaa0836be9bfbc8f-1024x141.jpg\" alt=\"\u56f31\uff1a\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u89e3\u6790\u306e\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u4f8b\" width=\"760\" height=\"105\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/11\/55bdb9631d844e9feaa0836be9bfbc8f-1024x141.jpg 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/11\/55bdb9631d844e9feaa0836be9bfbc8f-300x41.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/11\/55bdb9631d844e9feaa0836be9bfbc8f-768x106.jpg 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/11\/55bdb9631d844e9feaa0836be9bfbc8f.jpg 1226w\" sizes=\"auto, (max-width: 760px) 100vw, 760px\" \/><p id=\"caption-attachment-15696\" class=\"wp-caption-text\">\u56f31\uff1a\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u89e3\u6790\u306e\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u4f8b<\/p><\/div>\n<p>\u591a\u304f\u306eDNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306f\u30012\u672c\u9396DNA\u306e3\u00b4\u672b\u7aef\u306b\u30a2\u30c7\u30ce\u30b7\u30f3\u3092\u4ed8\u52a0\u3059\u308b\u6027\u8cea\u304c\u3042\u308a\u307e\u3059\u3002PCR\u306e\u5de5\u7a0b\u3067\u3001\u3053\u306e\u30a2\u30c7\u30ce\u30b7\u30f3\u306e\u4ed8\u52a0\u304c\u8d77\u3053\u308b\u3068\u3001\u96fb\u6c17\u6cf3\u52d5\u306e\u7d50\u679c\u5f97\u3089\u308c\u308b\u30a8\u30ec\u30af\u30c8\u30ed\u30d5\u30a7\u30ed\u30b0\u30e9\u30e0\u3067\u306f\u30011\u5869\u57fa\u5927\u304d\u306a\u30b5\u30a4\u30ba\u5024\u306b\u30d4\u30fc\u30af\u304c\u691c\u51fa\u3055\u308c\u307e\u3059\u3002\u3053\u306e\u30d4\u30fc\u30af\u306fPlus A\uff08+A\uff09\u30d4\u30fc\u30af\u3068\u547c\u3070\u308c\u307e\u3059\u3002\u4ed8\u52a0\u52b9\u7387\u306f\u3001\u92f3\u578b\u306e5\u00b4\u672b\u7aef\u306e\u914d\u5217\u306b\u3082\u4f9d\u5b58\u3059\u308b\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u304a\u308a\u3001\u914d\u5217\u306b\u3088\u3063\u3066\u306f\u3001\u4ed8\u52a0\u3055\u308c\u306a\u304b\u3063\u305fDNA\u306e\u30d4\u30fc\u30af\uff08-A\u30d4\u30fc\u30af\uff09\u3068\u3001+A\u30d4\u30fc\u30af\u306e\u30c0\u30d6\u30eb\u30d4\u30fc\u30af\u3068\u306a\u308a\u3001\u7d50\u679c\u304c\u4e0d\u6b63\u78ba\u306b\u306a\u308b\u6050\u308c\u304c\u3042\u308a\u307e\u3059\u3002\u3053\u306e\u5bfe\u7b56\u306e1\u3064\u306b\u30015\u00b4\u672b\u7aef\u306bTail\u914d\u5217\u3092\u6301\u3064\u30ea\u30d0\u30fc\u30b9\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u5229\u7528\u304c\u3042\u308a\u307e\u3059\u3002Tail\u914d\u5217\u306f3\u00b4\u672b\u7aef\u3078\u306e\u30a2\u30c7\u30ce\u30b7\u30f3\u306e\u4ed8\u52a0\u3092\u4fc3\u30597\u5869\u57fa\u306e\u914d\u5217\u3067\u3042\u308a\u3001Tail\u914d\u5217\u3092\u542b\u3080\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u7528\u3044\u308b\u3053\u3068\u3067\u3001+A\u306e\u5358\u4e00\u30d4\u30fc\u30af\u306b\u8fd1\u3065\u3051\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<br \/>\n\u5f53\u793e\u30e9\u30dc\u3067\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u89e3\u6790\u306e\u5b9f\u9a13\u7cfb\u3092\u691c\u8a0e\u3057\u3066\u3044\u305f\u3068\u3053\u308d\u3001\u540c\u3058PCR\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u7528\u3044\u3066\u3082\u3001PCR\u8a66\u85ac\u3084\u6df7\u305c\u308b\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u6570\uff08\u91cf\uff09\u306b\u3088\u308a\u3001+A\u30d4\u30fc\u30af\u306e\u691c\u51fa\u72b6\u6cc1\u304c\u7570\u306a\u308b\u7d50\u679c\u304c\u5f97\u3089\u308c\u307e\u3057\u305f\u3002\u3082\u3057\u304b\u3057\u305f\u3089\u3001\u540c\u3058\u72b6\u6cc1\u306b\u906d\u9047\u3059\u308b\u65b9\u304c\u3044\u308b\u306e\u3067\u306f\u3001\u3068\u601d\u3044\u3001\u4eca\u56de\u306f\u3001PCR\u306e\u53cd\u5fdc\u6761\u4ef6\u306b\u3088\u308b+A\u30d4\u30fc\u30af\u306e\u9055\u3044\u306b\u3064\u3044\u3066\u306e\u5b9f\u9a13\u7d50\u679c\u3092\u307e\u3068\u3081\u307e\u3057\u305f\u3002<\/p>\n<h2>\u5b9f\u9a13<\/h2>\n<h3>\u4f7f\u7528\u8a66\u85ac<\/h3>\n<ul>\n<li>Applied Biosystems\u2122 <a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/403062?SID=srch-srp-403062?CID=gsd_cap_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_og_s00_pcr_fragment_workshop_gsd_ts_1_Social_LAB\" target=\"_blank\" rel=\"noopener\">Control DNA (from CEPH Individual 1347-02)<\/a><\/li>\n<li>Applied Biosystems\u2122 <a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/jp\/ja\/4398881?CID=gsd_cap_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_og_s00_pcr_fragment_workshop_gsd_ts_1_Social_LAB\" target=\"_blank\" rel=\"noopener\">AmpliTaq Gold\u2122 360 Master Mix<\/a><\/li>\n<li>Applied Biosystems\u2122 <a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/4464268?SID=srch-srp-4464268?CID=gsd_cap_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_og_s00_pcr_fragment_workshop_gsd_ts_1_Social_LAB\" target=\"_blank\" rel=\"noopener\">Platinum\u2122 Multiplex PCR Master Mix<\/a><\/li>\n<li>PCR\u30d7\u30e9\u30a4\u30de\u30fc\u30da\u30a2 4\u7a2e\uff08\u30d5\u30a9\u30ef\u30fc\u30c9\u30d7\u30e9\u30a4\u30de\u30fc\u306e5&#8217;\u672b\u7aef\u306b\u86cd\u5149\u6a19\u8b58\u3001\u30ea\u30d0\u30fc\u30b9\u30d7\u30e9\u30a4\u30de\u30fc\u306e5&#8217;\u672b\u7aef\u306bTail\u914d\u5217\u4ed8\u52a0\uff09<\/li>\n<li>Applied Biosystems\u2122 GeneScan\u2122 600 LIZ\u2122 dye Size Standard v2.0<\/li>\n<li>Applied Biosystems\u2122 Hi-Di\u2122 \u30db\u30eb\u30e0\u30a2\u30df\u30c9<\/li>\n<\/ul>\n<h3>\u4f7f\u7528\u6a5f\u5668<\/h3>\n<ul>\n<li>\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\uff1aApplied Biosystems\u2122 <a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/A36958?SID=srch-srp-A36958?CID=gsd_cap_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_og_s00_pcr_fragment_workshop_gsd_ts_1_Social_LAB\" target=\"_blank\" rel=\"noopener\">ProFlex\u2122 PCR System<\/a>\u3001Veriti\u2122 \u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc<\/li>\n<li>\u30b8\u30a7\u30cd\u30c6\u30a3\u30c3\u30af\u30a2\u30ca\u30e9\u30a4\u30b6\uff1aApplied Biosystems\u2122 SeqStudio\u2122 \u30b8\u30a7\u30cd\u30c6\u30a3\u30c3\u30af\u30a2\u30ca\u30e9\u30a4\u30b6<\/li>\n<\/ul>\n<h3>\u5b9f\u9a13<\/h3>\n<p>Control DNA CEPH 1347-02\u3092\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8DNA\u3068\u3057\u30014\u3064\u306e\u30de\u30a4\u30af\u30ed\u30b5\u30c6\u30e9\u30a4\u30c8\u30de\u30fc\u30ab\u30fc\uff08\u88681\uff09\u3092\u7528\u3044\u3066\u691c\u8a3c\u3092\u884c\u3044\u307e\u3057\u305f\u3002<\/p>\n<table>\n<caption><b>\u88681\uff1a\u30de\u30fc\u30ab\u30fc\u540d\u3068\u6a19\u8b58\u86cd\u5149\u8272\u7d20<\/b><\/caption>\n<tbody>\n<tr bgcolor=\"mediumblue\">\n<td style=\"text-align: center\" width=\"83\"><span style=\"color: #ffffff\"><b>\u30de\u30fc\u30ab\u30fc<\/b><\/span><\/td>\n<td style=\"text-align: center\" width=\"112\"><span style=\"color: #ffffff\"><b>\u86cd\u5149<\/b><\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"83\">A<\/td>\n<td style=\"text-align: center\" width=\"112\">6-FAM\u2122 \u8272\u7d20<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"83\">B<\/td>\n<td style=\"text-align: center\" width=\"112\">PET\u2122 \u8272\u7d20<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"83\">C<\/td>\n<td style=\"text-align: center\" width=\"112\">NED\u2122 \u8272\u7d20<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"83\">D<\/td>\n<td style=\"text-align: center\" width=\"112\">VIC\u2122 \u8272\u7d20<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>\uff1cPCR\uff1e<br \/>\nPCR\u8a66\u85ac\u306f\u3001AmpliTaq Gold 360 Master Mix\uff08\u4ee5\u4e0b\u3001AmpliTaq Gold 360\uff09\u3068\u3001Platinum Multiplex PCR Master Mix\uff08\u4ee5\u4e0b\u3001Platinum Multiplex\uff09\u3092\u7528\u3044\u3001\u305d\u308c\u305e\u308c\u3001\u88682\u3001\u88683\u306e\u7d44\u6210\u3067\u53cd\u5fdc\u6db2\u3092\u8abf\u88fd\u3057\u307e\u3057\u305f\u3002Platinum Multiplex\u3067\u306f\u30011\u3064\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u30da\u30a2\u306e\u307f\u3092\u6dfb\u52a0\u3057\u305f\u53cd\u5fdc\u6db2\u3068\u30014\u3064\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u30da\u30a2\u5168\u3066\u3092\u6df7\u5408\u3057\u305f\u53cd\u5fdc\u6db2\u306e\u30012\u7a2e\u306e\u53cd\u5fdc\u6db2\u3092\u7528\u610f\u3057\u307e\u3057\u305f\u3002PCR\u306e\u6e29\u5ea6\u6761\u4ef6\u306f\u3001\u88684 \uff08AmpliTaq Gold 360\uff09\u3001\u88685\uff08Platinum Multiplex\uff09\u306b\u793a\u3057\u305f\u901a\u308a\u3067\u3059\u3002\u306a\u304a\u3001\u53cd\u5fdc\u6db2\u306e\u7d44\u6210\u3001PCR\u6761\u4ef6\u306f\u3001AmpliTaq Gold 360\u304a\u3088\u3073Platinum Multiplex\u306e\u63a8\u5968\u6761\u4ef6<sup>[1, 2]<\/sup>\u3092\u53c2\u8003\u306b\u6c7a\u5b9a\u3057\u307e\u3057\u305f\u3002<\/p>\n<table>\n<caption><b>\u88682 AmpliTaq Gold 360\u3092\u7528\u3044\u305f\u53cd\u5fdc\u6db2\u7d44\u6210<\/b><\/caption>\n<tbody>\n<tr bgcolor=\"mediumblue\">\n<td style=\"text-align: center\" width=\"223\"><span style=\"color: #ffffff\"><b>\u7d44\u6210<\/b><\/span><\/td>\n<td style=\"text-align: center\" width=\"113\"><span style=\"color: #ffffff\"><b>\u5bb9\u91cf\/\u53cd\u5fdc<\/b><\/span><\/td>\n<td style=\"text-align: center\" width=\"151\"><span style=\"color: #ffffff\"><b>\u7d42\u6fc3\u5ea6<\/b><\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"223\">PCR Master Mix (X2)<\/td>\n<td style=\"text-align: center\" width=\"113\">12.5 \u00b5L<\/td>\n<td style=\"text-align: center\" width=\"151\">X1<\/td>\n<\/tr>\n<tr>\n<td width=\"223\">Template DNA (10 ng\/\u00b5L)<\/td>\n<td style=\"text-align: center\" width=\"113\">1 \u00b5L<\/td>\n<td style=\"text-align: center\" width=\"151\">0.4 ng\/\u00b5L<\/td>\n<\/tr>\n<tr>\n<td width=\"223\">Primer pair (10 \u00b5M each) <sup>\u203b1<\/sup><\/td>\n<td style=\"text-align: center\" width=\"113\">1 \u00b5L<\/td>\n<td style=\"text-align: center\" width=\"151\">400 nM each<\/td>\n<\/tr>\n<tr>\n<td width=\"223\">Nuclease Free Water<\/td>\n<td style=\"text-align: center\" width=\"113\">10.5 \u00b5L<\/td>\n<td style=\"text-align: center\" width=\"151\">&#8211;<\/td>\n<\/tr>\n<tr>\n<td width=\"223\">Total<\/td>\n<td style=\"text-align: center\" width=\"113\">25 \u00b5L<\/td>\n<td style=\"text-align: center\" width=\"151\">&#8211;<\/td>\n<\/tr>\n<\/tbody>\n<tfoot>\n<tr>\n<td colspan=\"3\"><span style=\"font-size: 12px\">\u203b1 : 1\u30d7\u30e9\u30a4\u30de\u30fc\u30da\u30a2\/\u53cd\u5fdc\u6db2<\/span><\/td>\n<\/tr>\n<\/tfoot>\n<\/table>\n<table>\n<caption><b>\u88683 Platinum Multiplex\u3092\u7528\u3044\u305f\u53cd\u5fdc\u6db2\u7d44\u6210<\/b><\/caption>\n<tbody>\n<tr bgcolor=\"mediumblue\">\n<td style=\"text-align: center\" width=\"223\"><span style=\"color: #ffffff\"><b>\u7d44\u6210<\/b><\/span><\/td>\n<td style=\"text-align: center\" width=\"113\"><span style=\"color: #ffffff\"><b>\u5bb9\u91cf\/\u53cd\u5fdc<\/b><\/span><\/td>\n<td style=\"text-align: center\" width=\"151\"><span style=\"color: #ffffff\"><b>\u7d42\u6fc3\u5ea6<\/b><\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"223\">PCR Master Mix (X2)<\/td>\n<td style=\"text-align: center\" width=\"113\">12.5 \u00b5L<\/td>\n<td style=\"text-align: center\" width=\"151\">X1<\/td>\n<\/tr>\n<tr>\n<td width=\"223\">Template DNA (50 ng\/\u00b5L)<\/td>\n<td style=\"text-align: center\" width=\"113\">1 \u00b5L<\/td>\n<td style=\"text-align: center\" width=\"151\">2 ng\/\u00b5L<\/td>\n<\/tr>\n<tr>\n<td width=\"223\">Primer pair ((0.5~1 \u00b5M each) <sup>\u203b2<\/sup><\/td>\n<td style=\"text-align: center\" width=\"113\">2.5 \u00b5L<\/td>\n<td style=\"text-align: center\" width=\"151\">50~100 nM each<\/td>\n<\/tr>\n<tr>\n<td width=\"223\">Nuclease Free Water<\/td>\n<td style=\"text-align: center\" width=\"113\">9 \u00b5L<\/td>\n<td style=\"text-align: center\" width=\"151\">&#8211;<\/td>\n<\/tr>\n<tr>\n<td width=\"223\">Total<\/td>\n<td style=\"text-align: center\" width=\"113\">25 \u00b5L<\/td>\n<td style=\"text-align: center\" width=\"151\">&#8211;<\/td>\n<\/tr>\n<\/tbody>\n<tfoot>\n<tr>\n<td colspan=\"3\"><span style=\"font-size: 12px\">\u203b2 : 1\u30d7\u30e9\u30a4\u30de\u30fc\u30da\u30a2\/\u53cd\u5fdc\u6db2\u3001\u307e\u305f\u306f\u30014\u30d7\u30e9\u30a4\u30de\u30fc\u30da\u30a2\/\u53cd\u5fdc\u6db2<\/span><\/td>\n<\/tr>\n<\/tfoot>\n<\/table>\n<div><\/div>\n<table>\n<caption><b>\u88684 AmpliTaq Gold 360\u3092\u7528\u3044\u305fPCR\u6761\u4ef6<\/b><\/caption>\n<tbody>\n<tr bgcolor=\"mediumblue\">\n<td rowspan=\"2\" width=\"51\"><\/td>\n<td style=\"text-align: center\" colspan=\"6\" width=\"447\"><span style=\"color: #ffffff\"><b>Step<\/b><\/span><\/td>\n<\/tr>\n<tr bgcolor=\"mediumblue\">\n<td style=\"text-align: center\" width=\"75\"><span style=\"color: #ffffff\"><b>Hold<\/b><\/span><\/td>\n<td style=\"text-align: center\" colspan=\"3\" width=\"224\"><span style=\"color: #ffffff\"><b>30 Cycle<\/b><\/span><\/td>\n<td style=\"text-align: center\" width=\"75\"><span style=\"color: #ffffff\"><b>Hold<\/b><\/span><\/td>\n<td style=\"text-align: center\" width=\"75\"><span style=\"color: #ffffff\"><b>Hold<\/b><\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"51\">Temp.<\/td>\n<td style=\"text-align: center\" width=\"75\">95 \u2103<\/td>\n<td style=\"text-align: center\" width=\"75\">95 \u2103<\/td>\n<td style=\"text-align: center\" width=\"75\">60 \u2103<\/td>\n<td style=\"text-align: center\" width=\"75\">72 \u2103<\/td>\n<td style=\"text-align: center\" width=\"75\">72 \u2103<\/td>\n<td style=\"text-align: center\" width=\"75\">4 \u2103<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"51\">Time<\/td>\n<td style=\"text-align: center\" width=\"75\">10 min<\/td>\n<td style=\"text-align: center\" width=\"75\">30 sec<\/td>\n<td style=\"text-align: center\" width=\"75\">30 sec<\/td>\n<td style=\"text-align: center\" width=\"75\">1 min<\/td>\n<td style=\"text-align: center\" width=\"75\">7 min<\/td>\n<td style=\"text-align: center\" width=\"75\">\u221e<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<table>\n<caption><b>\u88685 Platinum Multiplex\u3092\u7528\u3044\u305fPCR\u6761\u4ef6<\/b><\/caption>\n<tbody>\n<tr bgcolor=\"mediumblue\">\n<td rowspan=\"2\" width=\"51\"><\/td>\n<td style=\"text-align: center\" colspan=\"6\" width=\"447\"><span style=\"color: #ffffff\"><b>Step<\/b><\/span><\/td>\n<\/tr>\n<tr bgcolor=\"mediumblue\">\n<td style=\"text-align: center\" width=\"75\"><span style=\"color: #ffffff\"><b>Hold<\/b><\/span><\/td>\n<td style=\"text-align: center\" colspan=\"3\" width=\"224\"><span style=\"color: #ffffff\"><b>30 Cycle<\/b><\/span><\/td>\n<td style=\"text-align: center\" width=\"75\"><span style=\"color: #ffffff\"><b>Hold<\/b><\/span><\/td>\n<td style=\"text-align: center\" width=\"75\"><span style=\"color: #ffffff\"><b>Hold<\/b><\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"51\">Temp.<\/td>\n<td style=\"text-align: center\" width=\"75\">95 \u2103<\/td>\n<td style=\"text-align: center\" width=\"75\">95 \u2103<\/td>\n<td style=\"text-align: center\" width=\"75\">60 \u2103<\/td>\n<td style=\"text-align: center\" width=\"75\">72 \u2103<\/td>\n<td style=\"text-align: center\" width=\"75\">60 \u2103<\/td>\n<td style=\"text-align: center\" width=\"75\">4 \u2103<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"51\">Time<\/td>\n<td style=\"text-align: center\" width=\"75\">2 min<\/td>\n<td style=\"text-align: center\" width=\"75\">30 sec<\/td>\n<td style=\"text-align: center\" width=\"75\">1min 30 sec<\/td>\n<td style=\"text-align: center\" width=\"75\">1 min<\/td>\n<td style=\"text-align: center\" width=\"75\">30 min<\/td>\n<td style=\"text-align: center\" width=\"75\">\u221e<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>\uff1c\u6cf3\u52d5\u30b5\u30f3\u30d7\u30eb\u306e\u8abf\u88fd\uff1e<br \/>\n\u5e0c\u91c8\u3057\u305fPCR\u7523\u7269\u3092\u3001\u4e0b\u8868\u306e\u7d44\u6210\u3067\u3001\u30b5\u30a4\u30ba\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\u3001Hi-Di\u30db\u30eb\u30e0\u30a2\u30df\u30c9\u3068\u6df7\u5408\u3057\u3001\u6cf3\u52d5\u30b5\u30f3\u30d7\u30eb\u3068\u3057\u307e\u3057\u305f\u3002<\/p>\n<table>\n<tbody>\n<tr bgcolor=\"mediumblue\">\n<td style=\"text-align: center\" width=\"300\"><span style=\"color: #ffffff\"><b>Component<\/b><\/span><\/td>\n<td style=\"text-align: center\" width=\"100\"><span style=\"color: #ffffff\"><b>Volume<\/b><\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"300\">PCR sample<\/td>\n<td style=\"text-align: center\" width=\"100\">1 \u00b5L<\/td>\n<\/tr>\n<tr>\n<td width=\"300\">GeneScan 600LIZ dye Size Standard v2.0<\/td>\n<td style=\"text-align: center\" width=\"100\">0.2 \u00b5L<\/td>\n<\/tr>\n<tr>\n<td width=\"300\">Hi-Di Formamide<\/td>\n<td style=\"text-align: center\" width=\"100\">9.8 \u00b5L<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>\uff1c\u96fb\u6c17\u6cf3\u52d5\uff1e<br \/>\n\u30e9\u30f3\u30e2\u30b8\u30e5\u30fc\u30eb\u306fFragAnalysis\u3001Dye Set\u306fG5\u306e\u8a2d\u5b9a\u3067\u3001\u96fb\u6c17\u6cf3\u52d5\u3092\u884c\u3044\u307e\u3057\u305f\u3002<\/p>\n<h2>\u7d50\u679c<\/h2>\n<p>\u5404\u30de\u30a4\u30af\u30ed\u30b5\u30c6\u30e9\u30a4\u30c8\u30de\u30fc\u30ab\u30fc\u306e\u30a8\u30ec\u30af\u30c8\u30ed\u30d5\u30a7\u30ed\u30b0\u30e9\u30e0\u3092\u56f32\u306b\u793a\u3057\u307e\u3059\u3002AmpliTaq Glod 360\u3092\u7528\u3044\u305f\u5834\u5408\uff08\u56f32 \u4e0a\u6bb5\uff09\u3001\u3044\u305a\u308c\u306e\u30de\u30fc\u30ab\u30fc\u3067\u3082\u3001\u30e1\u30a4\u30f3\u306e\u30a2\u30ec\u30eb\u30d4\u30fc\u30af\u306b\u5bfe\u3057\u30012 bp\u305a\u3064\u5c0f\u3055\u3044\u30b5\u30a4\u30ba\u5024\u306b\u30d4\u30fc\u30af\u304c\u691c\u51fa\u3055\u308c\u307e\u3057\u305f\uff08\u56f32 \uff0a\u3067\u8868\u793a\uff09\u3002\u30de\u30fc\u30ab\u30fcA\uff5eD\u306f\u3044\u305a\u308c\u3082\u30012\u5869\u57fa\u5358\u4f4d\u306e\u7e70\u308a\u8fd4\u3057\u914d\u5217\u3092\u542b\u307f\u307e\u3059\u3002\u3053\u306e\u3088\u3046\u306a\u30de\u30fc\u30ab\u30fc\u306ePCR\u3092\u884c\u3046\u3068\u3001\u30e1\u30a4\u30f3\u306e\u30a2\u30ec\u30eb\u306ePCR\u7523\u7269\u306b\u6bd4\u3079\u30662\u5869\u57fa\u5358\u4f4d\uff082\u30014\u3001\u2026\u3001\u5869\u57fa\uff09\u5206\u77ed\u3044\u65ad\u7247\u304c\u526f\u7523\u7269\u3068\u3057\u3066\u751f\u3058\u3001\u30d4\u30fc\u30af\u3068\u3057\u3066\u691c\u51fa\u3055\u308c\u307e\u3059\u3002\u3053\u308c\u3089\u306f\u3001\u30b9\u30bf\u30c3\u30bf\u30fc\u30d4\u30fc\u30af\u3068\u547c\u3070\u308c\u307e\u3059\u3002\u307e\u305f\u3001\u30b5\u30f3\u30d7\u30eb\u304c\u30d8\u30c6\u30ed\u3067\u3001\u4e21\u30a2\u30ec\u30eb\u306e\u7e70\u308a\u8fd4\u3057\u6570\u304c1\u3064\u9055\u3044\u306e\u5834\u5408\u3001\u5c0f\u3055\u3044\u65b9\u306e\u30a2\u30ec\u30eb\u30d4\u30fc\u30af\u306b\u5927\u304d\u3044\u65b9\u306e\u30b9\u30bf\u30c3\u30bf\u30fc\u30d4\u30fc\u30af\u304c\u91cd\u306a\u308a\u307e\u3059\u3002\u30de\u30fc\u30ab\u30fcD\u306e184.5 bp\u306e\u30d4\u30fc\u30af\u304c\u3001\u3053\u308c\u306b\u8a72\u5f53\u3057\u307e\u3059\u3002<\/p>\n<p>Platinum Multiplex\u3092\u7528\u3044\u30011\u53cd\u5fdc\u6db2\u306b1\u30d7\u30e9\u30a4\u30de\u30fc\u30da\u30a2\u3092\u6dfb\u52a0\u3057\u3066PCR\u3092\u884c\u3044\uff081\u30de\u30fc\u30ab\u30fc\/\u53cd\u5fdc\uff09\u5f97\u3089\u308c\u305f\u7d50\u679c\u3092\u3001\u56f32\u4e0b\u6bb5\u306b\u793a\u3057\u307e\u3059\u3002AmpliTaq Glod 360\u3092\u7528\u3044\u305f\u5834\u5408\u306b\u307f\u3089\u308c\u305f\u30d4\u30fc\u30af\u306b\u52a0\u3048\u3001\u3053\u308c\u3089\u306e\u30d4\u30fc\u30af\u3088\u308a1 bp\u5927\u304d\u3044\u30b5\u30a4\u30ba\u5024\u306b\u3082\u3001\u30d4\u30fc\u30af\u304c\u691c\u51fa\u3055\u308c\u307e\u3057\u305f\uff08\u56f32 \u25bd\u3067\u8868\u793a\uff09\u3002\u3053\u308c\u306f\u30013\u00b4\u672b\u7aef\u3078\u306e\u30a2\u30c7\u30ce\u30b7\u30f3\u4ed8\u52a0\u306b\u3088\u308a\u751f\u3058\u305f\u526f\u7523\u7269\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002\u3053\u308c\u306b\u5bfe\u3057\u30011\u53cd\u5fdc\u6db2\u306b4\u30d7\u30e9\u30a4\u30de\u30fc\u30da\u30a2\u3092\u6df7\u5408\u3057\u3066PCR\u3092\u884c\u3063\u305f\uff084\u30de\u30fc\u30ab\u30fc\/\u53cd\u5fdc\uff09\u7d50\u679c\u3001+1 bp\u306e\u30d4\u30fc\u30af\u5f37\u5ea6\u306f\u5927\u5e45\u306b\u4f4e\u6e1b\u3057\u3001AmpliTaq Glod 360\u3092\u7528\u3044\u305f\u5834\u5408\u3068\u307b\u307c\u540c\u69d8\u306e\u30a8\u30ec\u30af\u30c8\u30ed\u30d5\u30a7\u30ed\u30b0\u30e9\u30e0\u304c\u5f97\u3089\u308c\u307e\u3057\u305f\u3002<\/p>\n<div id=\"attachment_15703\" style=\"width: 1028px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-15703\" class=\"size-full wp-image-15703\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/11\/d587a9afb7db6bbfaaa31dea6e995b55.jpg\" alt=\"\u56f32\uff1a\u5404\u30de\u30a4\u30af\u30ed\u30b5\u30c6\u30e9\u30a4\u30c8\u30de\u30fc\u30ab\u30fc\u306e\u30a8\u30ec\u30af\u30c8\u30ed\u30d5\u30a7\u30ed\u30b0\u30e9\u30e0\uff08\u6a2a\u8ef8\uff1abp\uff09\" width=\"1018\" height=\"723\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/11\/d587a9afb7db6bbfaaa31dea6e995b55.jpg 1018w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/11\/d587a9afb7db6bbfaaa31dea6e995b55-300x213.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/11\/d587a9afb7db6bbfaaa31dea6e995b55-768x545.jpg 768w\" sizes=\"auto, (max-width: 1018px) 100vw, 1018px\" \/><p id=\"caption-attachment-15703\" class=\"wp-caption-text\">\u56f32\uff1a\u5404\u30de\u30a4\u30af\u30ed\u30b5\u30c6\u30e9\u30a4\u30c8\u30de\u30fc\u30ab\u30fc\u306e\u30a8\u30ec\u30af\u30c8\u30ed\u30d5\u30a7\u30ed\u30b0\u30e9\u30e0\uff08\u6a2a\u8ef8\uff1abp\uff09<br \/>\uff0a\u3067\u793a\u3057\u305f\u30d4\u30fc\u30af\u9593\u306e\u30b5\u30a4\u30ba\u5024\u306e\u5dee\u7570\u306f2 bp<br \/>\u25bd\u3067\u793a\u3057\u305f\u30d4\u30fc\u30af\u306e\u30b5\u30a4\u30ba\u5024\u306f\u3001\u6ce2\u5f62\u4e2d\u306e\u5de6\u96a3\u306e\u30d4\u30fc\u30af\u30b5\u30a4\u30ba\u5024\u306b\u5bfe\u3057\uff0b1 bp<\/p><\/div>\n<p>\u4eca\u56de\u306e\u5b9f\u9a13\u3067\u306f\u3001Tail\u914d\u5217\u3092\u542b\u3080PCR\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u7528\u3044\u305f\u305f\u3081\u3001-A\u30d4\u30fc\u30af\u306f\u751f\u3058\u306b\u304f\u3044\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002\u3053\u308c\u3088\u308a\u3001AmpliTaq Gold 360\u3092\u7528\u3044\u305f\u7d50\u679c\u3067\u691c\u51fa\u3055\u308c\u305f\u30d4\u30fc\u30af\u306f+A\u30d4\u30fc\u30af\u3067\u3042\u308a\u3001Platinum Multiplex\u3067\u307f\u3089\u308c\u305f\u30011 bp\u5927\u304d\u306a\u30b5\u30a4\u30ba\u5024\u306e\u30d4\u30fc\u30af\uff08\u56f32\u3000\u25bd\uff09\u306f\u3001\u3082\u30461\u3064\u30a2\u30c7\u30ce\u30b7\u30f3\u304c\u4ed8\u52a0\u3055\u308c\u305f\u30d4\u30fc\u30af\uff08\u4ee5\u4e0b\u3001+AA\u30d4\u30fc\u30af\uff09\u3067\u3042\u308b\u3068\u63a8\u6e2c\u3055\u308c\u307e\u3059\u3002Platinum Multiplex\u3092\u7528\u3044\u30011\u30de\u30fc\u30ab\u30fc\u306e\u307f\u306ePCR\u3092\u884c\u3063\u305f\u5834\u5408\u306f\u3001\u53cd\u5fdc\u57fa\u8cea\u304c\u904e\u5270\u3068\u306a\u308a+AA\u30d4\u30fc\u30af\u304c\u9ad8\u3044\u30b7\u30b0\u30ca\u30eb\u5f37\u5ea6\u3067\u691c\u51fa\u3055\u308c\u30014\u30de\u30fc\u30ab\u30fc\/\u53cd\u5fdc\u3067\u306ePCR\u3067\u306f\u3001\u53cd\u5fdc\u6db2\u4e2d\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u7dcf\u91cf\u304c\u5897\u3048\u305f\u3053\u3068\u3067\u57fa\u8cea\u304c\u6d88\u8cbb\u3055\u308c\u305f\u305f\u3081\u3001+AA\u30d4\u30fc\u30af\u304c\u4f4e\u6e1b\u3057\u305f\u3068\u8003\u3048\u3066\u3044\u307e\u3059\u3002<\/p>\n<h2>\u307e\u3068\u3081<\/h2>\n<p>\u5b9f\u9a13\u306e\u7d50\u679c\u3001PCR\u8a66\u85ac\u3084\u30d7\u30e9\u30a4\u30de\u30fc\u7dcf\u91cf\u306e\u9055\u3044\u306b\u3088\u308a\u3001+A\u30d4\u30fc\u30af\u306e\u691c\u51fa\u72b6\u6cc1\u306f\u7570\u306a\u308b\u3053\u3068\u304c\u5206\u304b\u308a\u307e\u3057\u305f\u3002\u3055\u3089\u306b\u3001Tail\u914d\u5217\u3092\u542b\u3080\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u7528\u3044\u3001\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9PCR\u306b\u9069\u3057\u305f\u6761\u4ef6\u3067PCR\u3092\u884c\u3046\u3068\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u7dcf\u91cf\u304c\u5c11\u306a\u3044\u5834\u5408\u306f\u3001+AA\u30d4\u30fc\u30af\u304c\u691c\u51fa\u3055\u308c\u308b\u53ef\u80fd\u6027\u304c\u3042\u308b\u3053\u3068\u304c\u793a\u5506\u3055\u308c\u307e\u3057\u305f\u3002\u3053\u306e\u3088\u3046\u306a\u5834\u5408\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u7dcf\u91cf\u3092\u5897\u3084\u3059\u3053\u3068\u304c\u3001+AA\u30d4\u30fc\u30af\u3092\u4f4e\u6e1b\u3055\u305b\u3001+A\u306e\u5358\u4e00\u30d4\u30fc\u30af\u306b\u8fd1\u3065\u3051\u308b\u5bfe\u51e6\u6cd5\u306e1\u3064\u306b\u306a\u308b\u3053\u3068\u304c\u3001\u4eca\u56de\u306e\u5b9f\u9a13\u7d50\u679c\u304b\u3089\u5206\u304b\u308a\u307e\u3057\u305f\u3002<\/p>\n<h3>\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u89e3\u6790\u30e6\u30fc\u30b6\u30fc\u30ac\u30a4\u30c9<\/h3>\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/fragment-analysis-guide-registration-form.html?CID=gsd_cap_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_og_s00_pcr_fragment_workshop_gsd_ts_1_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/p>\n<p>\u30b5\u30f3\u30ac\u30fcDNA\u30b7\u30fc\u30b1\u30f3\u30b5\u306e\u30c7\u30e2\u3001\u8cc7\u6599\u3001\u898b\u7a4d\u3082\u308a\u306a\u3069\u306b\u3054\u8208\u5473\u304c\u3042\u308a\u307e\u3057\u305f\u3089\u3001\u4e0b\u8a18\u306e\u30ea\u30f3\u30af\u304b\u3089\u304a\u6c17\u8efd\u306b\u304a\u554f\u3044\u5408\u308f\u305b\u304f\u3060\u3055\u3044\u3002<br \/>\n<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/pcr-inquiry.html?CID=gsd_cap_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_og_s00_pcr_fragment_workshop_gsd_ts_1_Social_LAB\" target=\"_blank\" rel=\"noopener\">https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/pcr-inquiry.html<\/a><\/p>\n<h2>\u53c2\u8003\u8cc7\u6599<\/h2>\n<p>1. <a href=\"https:\/\/www.thermofisher.com\/document-connect\/document-connect.html?url=https:\/\/assets.thermofisher.com\/TFS-Assets%2FLSG%2Fmanuals%2Fcms_061445.pdf&amp;CID=gsd_cap_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_og_s00_pcr_fragment_workshop_gsd_ts_1_Social_LAB\" target=\"_blank\" rel=\"noopener\">AmpliTaq Gold\u00ae 360 Master Mix Protocol<\/a><br \/>\n2. <a href=\"https:\/\/www.thermofisher.com\/document-connect\/document-connect.html?url=https:\/\/assets.thermofisher.com\/TFS-Assets%2FLSG%2Fmanuals%2F4463722_PltinmMltiplx_UG.pdf&amp;CID=gsd_cap_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_og_s00_pcr_fragment_workshop_gsd_ts_1_Social_LAB\" target=\"_blank\" rel=\"noopener\">Platinum\u2122 Multiplex PCR Master Mix USER 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