{"id":1575,"date":"2019-03-14T00:00:00","date_gmt":"2019-03-13T15:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/user_voice_48_3_quantstudio\/"},"modified":"2023-12-27T16:49:17","modified_gmt":"2023-12-27T07:49:17","slug":"user_voice_48_3_quantstudio","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/user_voice_48_3_quantstudio\/","title":{"rendered":"\u30c7\u30b8\u30bf\u30ebPCR\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u3092\u7528\u3044\u305f\u8840\u4e2d\u5909\u7570TP53 \u306e\u9ad8\u611f\u5ea6\u691c\u51fa\u6cd5\u306e\u958b\u767a\uff08\u5ca9\u624b\u533b\u79d1\u5927\u5b66\u533b\u6b6f\u85ac\u7dcf\u5408\u7814\u7a76\u6240 \u897f\u585a\u54f2 \u6c0f\uff09-\u300cNEXT\u300d2018\u5e7412\u6708\u53f7\u63b2\u8f09"},"content":{"rendered":"<h2 style=\"text-align: left\"><span style=\"font-size: 12pt\"><strong>QuantStudio 3D\u30c7\u30b8\u30bf\u30ebPCR\u30b7\u30b9\u30c6\u30e0\u304c\u9ad8\u611f\u5ea6\u6e2c\u5b9a\u3092\u30b5\u30dd\u30fc\u30c8<\/strong><\/span><\/h2>\n<p><strong>\u897f\u585a\u54f2 \u6c0f\uff08 \u5ca9\u624b\u533b\u79d1\u5927\u5b66 \u533b\u6b6f\u85ac\u7dcf\u5408\u7814\u7a76\u6240\u533b\u7642\u958b\u767a\u7814\u7a76\u90e8\u9580 \u7279\u4efb\u6559\u6388\u30fb\u5199\u771f\u53f3\uff09<\/strong><\/p>\n<p><strong><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/DSC09460_1.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-5845 size-full alignleft\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/DSC09460_1.jpg\" alt=\"\" width=\"250\" height=\"165\" \/><\/a><\/strong>\u304c\u3093\u6cbb\u7642\u5f8c\u306e\u518d\u767a\u3092\u65e9\u671f\u306b\u691c\u77e5\u3059\u308b\u816b\u760d\u30de\u30fc\u30ab\u30fc\u306e\u63a2\u7d22\u7814\u7a76\u306b\u53d6\u308a\u7d44\u3080\u3001\u5ca9\u624b\u533b\u79d1\u5927\u5b66\u306e\u897f\u585a\u54f2\u6c0f\u3089\u306e\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u3002\u300c\u9032\u884c\u304c\u3093\u3092\u624b\u8853\u3067\u5207\u9664\u5f8c\u3001\u8840\u4e2d\u3092\u5faa\u74b0\u3059\u308b\u816b\u760d\u7531\u6765DNA\uff08ctDNA ; circulatingtumor DNA\uff09\u304b\u3089\u7279\u5b9a\u306e\u907a\u4f1d\u5b50\u5909\u7570\u3092\u9ad8\u611f\u5ea6\u306b\u691c\u51fa\u3057\u3001\u518d\u767a\u3092\u691c\u77e5\u3059\u308b\u7cfb\u3092\u958b\u767a\u4e2d\u3067\u3059\u3002\u30ea\u30ad\u30c3\u30c9\u30d0\u30a4\u30aa\u30d7\u30b7\u30fc\u306a\u306e\u3067\u3001\u60a3\u8005\u3055\u3093\u3078\u306e\u8ca0\u62c5\u3082\u8efd\u304f\u3066\u6e08\u307f\u307e\u3059\u300d\u3068\u8a71\u3059\u897f\u585a\u6c0f\u306b\u3001\u691c\u51fa\u7528\u30a2\u30c3\u30bb\u30a4\u306e\u958b\u767a\u3068QuantStudio 3D\u30c7\u30b8\u30bf\u30ebPCR\u30b7\u30b9\u30c6\u30e0\u306e\u6d3b\u7528\u306b\u3064\u3044\u3066\u4f3a\u3044\u307e\u3057\u305f\u3002<\/p>\n<h2><span style=\"font-size: 12pt\">0.1%\u306b\u6e80\u305f\u306a\u3044\u5909\u7570\u3092\u611f\u5ea6\u3088\u304f\u691c\u51fa\u3059\u308b\u306b\u306f?<\/span><\/h2>\n<p>\u7e70\u308a\u8fd4\u3057\u5b9f\u65bd\u3067\u304d\u3001\u5c11\u3057\u3067\u3082\u65e9\u304f\u518d\u767a\u3092\u691c\u77e5\u3059\u308b\u65b9\u6cd5\u3092\u7814\u7a76\u3057\u3066\u304d\u305f\u897f\u585a\u6c0f\u306f\u3001ctDNA\u306b\u7740\u76ee\u3057\u305f\u7406\u7531\u3092\u6b21\u306e\u3088\u3046\u306b\u8aac\u660e\u3057\u307e\u3059\u3002\u300c\u60a3\u8005\u3055\u3093\u3054\u3068\u306b\u5909\u7570\u3059\u308b\u907a\u4f1d\u5b50\u3084\u5909\u7570\u90e8\u4f4d\u306f\u7570\u306a\u308a\u307e\u3059\u3002\u6458\u51fa\u3057\u305f\u304c\u3093\u7d44\u7e54\u306e\u907a\u4f1d\u5b50\u5909\u7570\u3092\u6b21\u4e16\u4ee3\u30b7\u30fc\u30b1\u30f3\u30b5\u3067\u540c\u5b9a\u3057\u3001\u8853\u5f8c\u306f\u60a3\u8005\u3055\u3093\u7279\u6709\u306e\u5909\u7570\u3092\u8840\u4e2d\u306ectDNA\u3067\u691c\u51fa\u3067\u304d\u308c\u3070\u3001\u518d\u767a\u306e\u5146\u5019\u3092\u3044\u3061\u65e9\u304f\u691c\u77e5\u3067\u304d\u308b\u306f\u305a\u3002\u305f\u3060\u3057\u3001\u60a3\u8005\u3055\u3093\u306e\u8eab\u4f53\u7684\u306a\u8ca0\u62c5\u306f\u5c11\u306a\u304f\u3066\u3082\u3001\u9577\u671f\u9593\u884c\u3046\u5834\u5408\u304c\u3042\u308b\u306e\u3067\u3001\u691c\u51fa\u611f\u5ea6\u3068\u7d4c\u6e08\u6027\u306e\u4e21\u7acb\u306f\u91cd\u8981\u3067\u3059\u3002\u6b21\u4e16\u4ee3\u30b7\u30fc\u30b1\u30f3\u30b5\u306f\u5168\u3066\u306e\u5909\u7570\u3092\u4e00\u5ea6\u3067\u89e3\u6790\u3057\u307e\u3059\u304c\u3001\u5909\u7570\u30a2\u30ea\u30eb\u306e\u78ba\u5b9f\u306a\u691c\u51fa\u611f\u5ea6\u306f\u4e00\u822c\u7684\u306b\u6570%\u30aa\u30fc\u30c0\u30fc\u307e\u3067\u3002\u3053\u308c\u306b\u5bfe\u3057\u3066\u30c7\u30b8\u30bf\u30ebPCR\u3092\u4f7f\u3048\u3070\u30010.1%\u306b\u6e80\u305f\u306a\u3044\u30a2\u30ea\u30eb\u983b\u5ea6\u3082\u691c\u51fa\u53ef\u80fd\u3067\u3059\u3002\u98df\u9053\u304c\u3093\u3067\u5b9f\u9a13\u7684\u306b\u8abf\u3079\u305f\u3068\u3053\u308d\u3001CT\u306b\u3088\u308b\u753b\u50cf\u8a3a\u65ad\u3067\u30ea\u30f3\u30d1\u7bc0\u8ee2\u79fb\u3092\u691c\u51fa\u3059\u308b\u3088\u308a\u3082180\u65e5\u7a0b\u65e9\u304fctDNA\u304b\u3089\u76ee\u7684\u306e\u907a\u4f1d\u5b50\u5909\u7570\u3092\u691c\u51fa\u3067\u304d\u307e\u3057\u305f\u3002\u307e\u305f\u3001\u3053\u306e\u75c7\u4f8b\u3067\u306f\u5f93\u6765\u306e\u8840\u6e05\u816b\u760d\u30de\u30fc\u30ab\u30fc\u3067\u306f\u518d\u767a\u3092\u691c\u51fa\u3067\u304d\u307e\u305b\u3093\u3067\u3057\u305f\u3002\u305f\u3060\u3057ctDNA\u306f\u8840\u6db2\u3092\u5faa\u74b0\u4e2d\u306b\u65ad\u7247\u5316\u3055\u308c\u3066100bp\u4ee5\u4e0b\u306b\u306a\u3063\u3066\u3044\u308b\u3053\u3068\u304c\u591a\u3044\u306e\u3067\u3001\u65ad\u7247\u5316\u3057\u3066\u3082\u6e2c\u5b9a\u3067\u304d\u308b\u30a2\u30c3\u30bb\u30a4\u7cfb\u304c\u5fc5\u8981\u3068\u306a\u308a\u307e\u3059\u3002\u79c1\u305f\u3061\u306f70bp\u7a0b\u5ea6\u306e\u77ed\u3044DNA\u3067\u30a2\u30c3\u30bb\u30a4\u3067\u304d\u308b\u7cfb\u3092\u69cb\u7bc9\u3057\u3001\u304c\u3093\u7d44\u7e54\u3067\u540c\u5b9a\u3055\u308c\u305f\u907a\u4f1d\u5b50\u5909\u7570\u306b\u5bfe\u3059\u308b70\u7a2e\u985e\u306e\u5909\u7570\u306b\u3064\u3044\u3066\u8a66\u3057\u305f\u3068\u3053\u308d\u300197%\u306f\u76ee\u7684\u306e\u5909\u7570\u3092\u9ad8\u611f\u5ea6\u306b\u691c\u51fa\u3067\u304d\u308b\u3053\u3068\u3092\u78ba\u8a8d\u3057\u307e\u3057\u305f\u300d\u3002<\/p>\n<h2><span style=\"font-size: 12pt\">\u816b\u760d\u30de\u30fc\u30ab\u30fc\u3068\u3057\u3066<span class=\"i\">TP53<\/span> \u907a\u4f1d\u5b50\u306e\u6709\u7528\u6027<\/span><\/h2>\n<p>\u300c<span class=\"i\">TP53<\/span> \u306e\u75c5\u7684\u5909\u7570\u306f\u5e45\u5e83\u3044\u304c\u3093\u3067\u89b3\u5bdf\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u7279\u306b\u79c1\u305f\u3061\u306e\u30b0\u30eb\u30fc\u30d7\u304c\u53d6\u308a\u7d44\u3080\u6d88\u5316\u5668\u304c\u3093\u3067\u306f\u3001\u98df\u9053\u304c\u3093\u3067\u307b\u307c100%\u3001\u5927\u8178\u304c\u3093\u3084\u80c3\u304c\u3093\u306e\u60a3\u8005\u3055\u3093\u3067\u306f\u7d0470%\u306e\u65b9\u306b<span class=\"i\">TP53<\/span> \u306e\u5909\u7570\u304c\u898b\u3089\u308c\u307e\u3059\u3002\u4ed6\u306b\u3082\u80ba\u304c\u3093\u3084\u5375\u5de3\u304c\u3093\u306a\u3069\u591a\u304f\u306e\u304c\u3093\u3067<span class=\"i\">TP53<\/span> \u306e\u30a2\u30c3\u30bb\u30a4\u304c\u4f7f\u3048\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u307e\u305f\u304c\u3093\u7d44\u7e54\u306f\u30d8\u30c6\u30ed\u4e0d\u5747\u4e00\u6027\u304c\u3042\u308a\u3001\u8907\u6570\u306e\u30b5\u30d6\u30af\u30ed\u30fc\u30f3\u3067\u69cb\u6210\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u304c\u3093\u767a\u751f\u306e\u521d\u671f\u306b\u8d77\u304d\u305f\u907a\u4f1d\u5b50\u5909\u7570\u306f\u3001\u30b5\u30d6\u30af\u30ed\u30fc\u30f3\u9593\u306b\u5171\u901a\u3057\u3066\u9ad8\u3044\u983b\u5ea6\u3092\u6709\u3059\u308b\u306e\u3067\u3001\u30de\u30fc\u30ab\u30fc\u3068\u3057\u3066\u6709\u7528\u3067\u3059\u3002\u79c1\u305f\u3061\u304c\u8abf\u3079\u305f\u691c\u4f53\u3067\u306f\u3001<span class=\"i\">TP53<\/span> \u306e\u5909\u7570\u306f\u30b5\u30d6\u30af\u30ed\u30fc\u30f3\u9593\u3067\u5171\u901a\u3057\u3066\u3044\u308b\u78ba\u7387\u304c\u9ad8\u304f\u3001\u3053\u306e\u70b9\u304b\u3089\u3082\u30de\u30fc\u30ab\u30fc\u306b\u9069\u3057\u3066\u3044\u308b\u3068\u8003\u3048\u307e\u3057\u305f\u300d\u3068\u897f\u585a\u6c0f\u3002\u3055\u3089\u306b\u300cCOSMIC\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9\u3067\u304c\u3093\u306b\u95a2\u9023\u3059\u308b\u4f53\u7d30\u80de\u5909\u7570\u3092\u8abf\u3079\u305f\u3068\u3053\u308d\u3001<span class=\"i\">TP53<\/span> \u306eDNA\u7d50\u5408\u30c9\u30e1\u30a4\u30f3\u3092\u30b3\u30fc\u30c9\u3059\u308b\u90e8\u4f4d\u306b\u306f1284\u7a2e\u985e\u306e\u5909\u7570\u304c\u5831\u544a\u3055\u308c\u3066\u3044\u307e\u3057\u305f\u3002\u591a\u304f\u306f\u30ec\u30a2\u30d0\u30ea\u30a2\u30f3\u30c8\u306a\u306e\u3067\u3001\u5909\u7570\u983b\u5ea6\u304c\u9ad8\u3044\u9806\u306b42\u7a2e\u985e\u306e\u30a2\u30c3\u30bb\u30a4\u7cfb\u3092\u6e96\u5099\u3059\u308c\u3070<span class=\"i\">TP53<\/span> \u5909\u7570\u306e\u7d0450%\u3092\u691c\u51fa\u3067\u304d\u3001132\u7a2e\u985e\u3067\u306f70%\u3001426\u7a2e\u985e\u3067\u306f90%\u307e\u3067\u3092\u691c\u51fa\u3067\u304d\u308b\u3053\u3068\u304c\u308f\u304b\u308a\u307e\u3057\u305f\u3002<span class=\"i\">TP53<\/span> \u306b\u5bfe\u3059\u308b\u9650\u3089\u308c\u305f\u6570\u306e\u30c7\u30b8\u30bf\u30ebPCR\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u3092\u6574\u5099\u3059\u308b\u3053\u3068\u3067\u3001\u5e45\u5e83\u3044\u304c\u3093\u306b\u5bfe\u5fdc\u3059\u308b\u7d4c\u6e08\u7684\u3067\u92ed\u654f\u306a\u691c\u51fa\u6cd5\u304c\u5b9f\u73fe\u3057\u307e\u3059\u300d\u3068\u7d9a\u3051\u307e\u3059\u3002<\/p>\n<h2><span style=\"font-size: 12pt\">\u6e2c\u5b9a\u306f\u3001\u4f7f\u3044\u3084\u3059\u3044QuantStudio3D\u30c7\u30b8\u30bf\u30ebPCR\u30b7\u30b9\u30c6\u30e0\u3067<\/span><\/h2>\n<p><em>\u524d\u8ff0\u306e\u98df\u9053\u304c\u3093\u306e\u7814\u7a76\u3092\u62c5\u5f53\u3057\u305f\u5916\u79d1\u5b66\u8b1b\u5e2b\u306e\u5ca9\u8c37\u5cb3\u6c0f\uff08\u5199\u771f\u5de6\uff09\u306f\u3001\u81e8\u5e8a\u30b5\u30f3\u30d7\u30eb\u3092\u542b\u3080\u591a\u304f\u306e\u89e3\u6790\u3092QuantStudio3D\u30c7\u30b8\u30bf\u30ebPCR\u30b7\u30b9\u30c6\u30e0\u3067\u884c\u3063\u3066\u3044\u307e\u3059\u3002\u300c\u3053\u306e\u30b7\u30b9\u30c6\u30e0\u306f\u3001\u64cd\u4f5c\u304c\u5bb9\u6613\u3067\u4f7f\u3044\u3084\u3059\u304f\u6570\u6642\u9593\u3067\u89e3\u6790\u7d50\u679c\u304c\u5f97\u3089\u308c\u308b\u305f\u3081\u3001\u81e8\u5e8a\u7814\u7a76\u306b\u9069\u3057\u3066\u3044\u308b\u3068\u601d\u3044\u307e\u3059\u3002\u6700\u592724\u30b5\u30f3\u30d7\u30eb\u3092\u4e00\u5ea6\u306b\u89e3\u6790\u53ef\u80fd\u3067\u3059\u304c\u3001\u53cd\u5fdc\u306f1\u30b5\u30f3\u30d7\u30eb1\u30c1\u30c3\u30d7\u3054\u3068\u306b\u884c\u3046\u306e\u3067\u30011\u691c\u4f53\u3060\u3051\u89e3\u6790\u3092\u884c\u3044\u305f\u3044\u5834\u5408\u306a\u3069\u3082\u30c1\u30c3\u30d7\u3084\u8a66\u85ac\u306e\u7121\u99c4\u304c\u306a\u304f\u52a9\u304b\u3063\u3066\u3044\u307e\u3059\u300d\u3068\u30b3\u30e1\u30f3\u30c8\u3057\u307e\u3059\u3002\u5ca9\u8c37\u6c0f\u3068\u897f\u585a\u6c0f\u306f\u3001\u300c\u3053\u306e\u30a2\u30c3\u30bb\u30a4\u3092\u3055\u3089\u306b\u5145\u5b9f\u3055\u305b\u3001\u4e00\u523b\u3082\u65e9\u304f\u81e8\u5e8a\u5fdc\u7528\u3078\u3064\u306a\u3052\u305f\u3044\u300d\u3068\u7814\u7a76\u3092\u9032\u3081\u3066\u3044\u307e\u3059\u3002<\/em><\/p>\n<h2><span style=\"font-size: 12pt\">\u30c1\u30c3\u30d7\u30d9\u30fc\u30b9\u306e\u30b3\u30f3\u30d1\u30af\u30c8\u306a\u30c7\u30b8\u30bf\u30ebPCR\u30b7\u30b9\u30c6\u30e0<\/span><br \/>\n<span style=\"font-size: 12pt\">QuantStudio 3D \u30c7\u30b8\u30bf\u30eb PCR \u30b7\u30b9\u30c6\u30e0<\/span><\/h2>\n<p><a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/pcr\/digital-pcr.html?CID=Social_LAB\"><img loading=\"lazy\" decoding=\"async\" class=\"alignleft wp-image-4298 size-medium\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/p9_QuantStudio_k-1-1-176x300.jpg\" alt=\"QuantStudio 3D \u30c7\u30b8\u30bf\u30ebPCR\u30b7\u30b9\u30c6\u30e0\" width=\"176\" height=\"300\" \/><\/a>Biosystems\u2122 QuantStudio\u2122 3D \u30c7\u30b8\u30bf\u30eb PCR \u30b7\u30b9\u30c6\u30e0\u306f\u3001<br 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