{"id":15898,"date":"2023-12-19T10:19:24","date_gmt":"2023-12-19T01:19:24","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/?p=15898"},"modified":"2025-07-02T16:53:45","modified_gmt":"2025-07-02T07:53:45","slug":"basic-workshop-gsd-ts-3","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/basic-workshop-gsd-ts-3\/","title":{"rendered":"\u3010\u3084\u3063\u3066\u307f\u305f\u3011\u5236\u9650\u9175\u7d20\u3067DNA\u3092\u5207\u65ad\u3059\u308b\u6642\u306b\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u3067\u3057\u3063\u304b\u308a\u6df7\u5408\u3057\u306a\u304b\u3063\u305f\u3089"},"content":{"rendered":"

\u30d0\u30a4\u30aa\u5b9f\u9a13\u3067\u306f\u3001\u30de\u30a4\u30af\u30ed\u30c1\u30e5\u30fc\u30d6\u306e\u4e2d\u3067\u6db2\u4f53\u8a66\u85ac\u3092\u6df7\u5408\u3059\u308b\u3053\u3068\u3067\u3001\u3055\u307e\u3056\u307e\u306a\u53cd\u5fdc\u3092\u9032\u3081\u307e\u3059\u3002\u6df7\u5408\u65b9\u6cd5\u306b\u306f\u3001\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u4ed6\u3001\u30dc\u30eb\u30c6\u30c3\u30af\u30b9\u3084\u30bf\u30c3\u30d4\u30f3\u30b0\u3001\u8ee2\u5012\u6df7\u548c\u306a\u3069\u304c\u6319\u3052\u3089\u308c\u307e\u3059\u3002\u9175\u7d20\u6d3b\u6027\u3078\u306e\u5f71\u97ff\u304c\u6c17\u306b\u306a\u308b\u5834\u9762\u3067\u306f\u3001\u6bd4\u8f03\u7684\u7a4f\u3084\u304b\u306b\u6df7\u5408\u3067\u304d\u308b\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u3092\u9078\u629e\u3055\u308c\u308b\u65b9\u3082\u591a\u3044\u306e\u3067\u306f\u306a\u3044\u3067\u3057\u3087\u3046\u304b\u3002\u904e\u53bb\u306e\u30d6\u30ed\u30b0<\/a>\u3067\u306f\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u30b3\u30c4\u3092\u52d5\u753b\u306b\u3057\u3066\u3054\u7d39\u4ecb\u3057\u307e\u3057\u305f\u304c\u3001\u5b9f\u969b\u306e\u5b9f\u9a13\u7d50\u679c\u306b\u306f\u3069\u306e\u3088\u3046\u306a\u5f71\u97ff\u304c\u3042\u308b\u306e\u3067\u3057\u3087\u3046\u304b\uff1f\u4eca\u56de\u306f\u5236\u9650\u9175\u7d20\u51e6\u7406\u306b\u3064\u3044\u3066\u3084\u3063\u3066\u307f\u307e\u3057\u305f\u3002\u904e\u53bb\u30d6\u30ed\u30b0<\/a>\u306e\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u52d5\u753b\u3082\u5408\u308f\u305b\u3066\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/p>\n

\u6750\u6599\u3068\u65b9\u6cd5<\/h2>\n

\u5236\u9650\u9175\u7d20\u51e6\u7406\u3059\u308b\u30bf\u30fc\u30b2\u30c3\u30c8\u3068\u3057\u3066\u30011042 bp\u306ePCR\u7523\u7269\u3092\u7528\u610f\u3057\u307e\u3057\u305f\u3002\u3053\u306ePCR\u7523\u7269\u306b\u306f\u3001\u5236\u9650\u9175\u7d20Hind III\u306e\u8a8d\u8b58\u30b5\u30a4\u30c8\u304c1\u30ab\u6240\u3060\u3051\u3042\u308a\u3001\u6b63\u3057\u304f\u51e6\u7406\u3055\u308c\u308c\u3070651 bp\u3068397 bp\u306e2\u3064\u306e\u65ad\u7247\u306b\u5207\u65ad\u3055\u308c\u308b\u306f\u305a\u3067\u3059\uff08\u56f31\uff09\u3002<\/p>\n

\"\u56f31.

\u56f31. \u5236\u9650\u9175\u7d20\u51e6\u7406\u5b9f\u9a13\u306b\u7528\u3044\u305fDNA<\/p><\/div>\n

\u5236\u9650\u9175\u7d20\u306e\u53cd\u5fdc\u6db2\u7d44\u6210\u306f\u88681\u306e\u901a\u308a\u3067\u3059\u3002\u5236\u9650\u9175\u7d20\u4ee5\u5916\u306e\u5171\u901a\u90e8\u5206\u306f\u307e\u3068\u3081\u3066\u8abf\u88fd\u3057\u3001\u6700\u5f8c\u306bHind III \u3082\u3057\u304f\u306fNuclease-Free Water\u3092\u6dfb\u52a0\u3057\u3066\u6df7\u5408\u3057\u307e\u3057\u305f\uff08\u88681\uff09\u3002<\/p>\n\n\n\n\n\n\n\n
\u88681\u3000\u5236\u9650\u9175\u7d20\u306e\u53cd\u5fdc\u6db2\u306e\u7d44\u6210<\/b><\/caption>\n
Nuclease-Free Water<\/td>\n15 \u00b5L<\/td>\n<\/tr>\n
Anza 10\u00d7 Buffer<\/td>\n2 \u00b5L<\/td>\n<\/tr>\n
PCR \u7523\u7269<\/td>\n2 \u00b5L<\/td>\n<\/tr>\n
Hind III or Nuclease-Free Water<\/td>\n1 \u00b5L<\/td>\n<\/tr>\n
Total<\/td>\n20 \u00b5L<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5236\u9650\u9175\u7d20\u306b\u3088\u308bDNA\u5207\u65ad\u306b\u3064\u3044\u3066\u3001\u6df7\u5408\u7a0b\u5ea6\u306b\u3088\u308b\u5f71\u97ff\u3092\u8a55\u4fa1\u3059\u308b\u305f\u3081\u30014\u3064\u306e\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u6761\u4ef6\uff08\u88682\uff09\u3067\u6df7\u5408\u3057\u3066\u304b\u3089\u5236\u9650\u9175\u7d20\u51e6\u7406\u3057\u3066\u307f\u307e\u3057\u305f\u3002\u88682\u306e\u300c\u30d4\u30da\u30c3\u30c8\u5bb9\u91cf\u300d\u306b\u8abf\u6574\u3057\u305f\u30d4\u30da\u30c3\u30c8\u3092\u6e96\u5099\u3057\u3001\u6761\u4ef61\u306fHind III\u306e\u4ee3\u308f\u308a\u306bNuclease-Free Water\u3092\u4f7f\u7528\u3057\u305f\u30cd\u30ac\u30c6\u30a3\u30d6\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u3001\u6761\u4ef62\u306f\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u3067\u3057\u3063\u304b\u308a\u6df7\u305c\u308b\u901a\u5e38\u6761\u4ef6\u3001\u6761\u4ef63\u306f\u5c11\u306a\u3059\u304e\u308b\u5bb9\u91cf\u3067\u6df7\u305c\u308b\u6761\u4ef6\u3001\u305d\u3057\u3066\u6761\u4ef64\u306f\u5168\u304f\u6df7\u305c\u306a\u304b\u3063\u305f\u5834\u5408\u3067\u3059\u3002\u9175\u7d20\u53cd\u5fdc\u3092\u9032\u3081\u308b\u305f\u308137\u2103\u306715\u5206\u9593\u51e6\u7406\u3057\u3001\u7d9a\u3051\u306695\u2103\u30675\u5206\u9593\u51e6\u7406\u3057\u3066\u5236\u9650\u9175\u7d20\u3092\u5931\u6d3b\u3055\u305b\u307e\u3057\u305f\u3002\u3053\u308c\u306f\u3001\u30a2\u30ac\u30ed\u30fc\u30b9\u30b2\u30eb\u96fb\u6c17\u6cf3\u52d5\u306e\u6e96\u5099\u4e2d\u306a\u3069\u306b\u53cd\u5fdc\u304c\u9032\u307e\u306a\u3044\u3088\u3046\u306b\u3059\u308b\u305f\u3081\u3067\u3059\u3002<\/p>\n\n\n\n\n\n\n\n
\u88682\u3000\u5236\u9650\u9175\u7d20\u306e\u53cd\u5fdc\u6db2\uff0820 \u00b5L\uff09\u306e\u6df7\u5408\u6761\u4ef6<\/b><\/caption>\n
\u6761\u4ef6<\/b><\/td>\n\u9175\u7d20<\/b><\/td>\n\u30d4\u30da\u30c3\u30c8\u5bb9\u91cf<\/b><\/td>\n\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u56de\u6570<\/b><\/td>\n<\/tr>\n
1<\/td>\nNuclease-Free Water<\/td>\n15 \u00b5L<\/td>\n5\u56de<\/td>\n<\/tr>\n
2<\/td>\nHind III<\/td>\n15 \u00b5L<\/td>\n5\u56de<\/td>\n<\/tr>\n
3<\/td>\nHind III<\/td>\n1 \u00b5L<\/td>\n5\u56de<\/td>\n<\/tr>\n
4<\/td>\nHind III<\/td>\nNA<\/td>\n0\u56de<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5236\u9650\u9175\u7d20\u3067DNA\u304c\u5207\u65ad\u3055\u308c\u305f\u304b\u3069\u3046\u304b\u306e\u78ba\u8a8d\u306f\u3001\u30a2\u30ac\u30ed\u30fc\u30b9\u30b2\u30eb\u96fb\u6c17\u6cf3\u52d5\u3067DNA\u306e\u30d0\u30f3\u30c9\u3092\u53ef\u8996\u5316\u3057\u3066\u65ad\u7247\u306e\u30b5\u30a4\u30ba\u304b\u3089\u5224\u65ad\u3057\u307e\u3057\u305f\u3002\u96fb\u6c17\u6cf3\u52d5\u306b\u306fInvitrogen\u2122 E-Gel Power Snap\u96fb\u6c17\u6cf3\u52d5\u30b7\u30b9\u30c6\u30e0<\/a>\u3068Invitrogen\u2122 E-Gel\u2122 EX \u30a2\u30ac\u30ed\u30fc\u30b9\u30b2\u30eb<\/a>\uff081%\uff09\u3092\u4f7f\u7528\u3057\u3001\u30b5\u30a4\u30ba\u30de\u30fc\u30ab\u30fc\u306b\u306fInvitrogen\u21221 Kb Plus DNA Ladder<\/a> \u3092\u4f7f\u7528\u3057\u307e\u3057\u305f\u3002<\/p>\n

\u7d50\u679c<\/h2>\n

\u305d\u308c\u3067\u306f\u7d50\u679c\u3092\u78ba\u8a8d\u3057\u3066\u307f\u307e\u3057\u3087\u3046\u3002
\n\u30ec\u30fc\u30f31\u306f\u5236\u9650\u9175\u7d20Hind III\u3092\u52a0\u3048\u306a\u3044\u30cd\u30ac\u30c6\u30a3\u30d6\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u306e\u305f\u3081\u3001PCR\u7523\u7269\u304c\u5207\u65ad\u3055\u308c\u3066\u304a\u3089\u305a1042 bp\u306eDNA\u306e\u30d0\u30f3\u30c9\u3092\u78ba\u8a8d\u3067\u304d\u307e\u3057\u305f\u3002\u30ec\u30fc\u30f32\u306fHind III\u3092\u52a0\u3048\u3066\u3057\u3063\u304b\u308a\u6df7\u5408\u3057\u305f\u6761\u4ef6\u3067\u3059\u306e\u3067\u3001\u4e88\u60f3\u3055\u308c\u305f\u901a\u308a651 bp\u3068397 bp\u7a0b\u5ea6\u306eDNA\u65ad\u7247\u3092\u78ba\u8a8d\u3067\u304d\u307e\u3057\u305f\u3002\u30ec\u30fc\u30f33\u306f1 \u00b5L\u5bb9\u91cf\u306e\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u3001\u30ec\u30fc\u30f34\u306f\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u3092\u5168\u304f\u3057\u306a\u3044\u6761\u4ef6\u3067\u3059\u3002\u3069\u3061\u3089\u306e\u30ec\u30fc\u30f3\u3082\u540c\u69d8\u306e\u7d50\u679c\u3067\u3001\u4e0a\u304b\u30891042 bp\u3001651 bp\u3001397 bp\u306e3\u672c\u306e\u30d0\u30f3\u30c9\u304c\u78ba\u8a8d\u3067\u304d\u307e\u3059\u3002\u610f\u5916\u306a\u3053\u3068\u306b\u3001\u5168\u304f\u53cd\u5fdc\u6db2\u3092\u6df7\u305c\u3066\u3044\u306a\u304f\u3066\u3082\u3001Hind III\u306b\u3088\u3063\u3066\u3042\u308b\u7a0b\u5ea6\u306f651 bp\u3068397 bp\u306eDNA\u65ad\u7247\u3078\u3068\u5207\u65ad\u3067\u304d\u308b\u3053\u3068\u304c\u5206\u304b\u308a\u307e\u3057\u305f\u3002\u3053\u308c\u306f\u3001Hind III\u3092\u52a0\u3048\u3066\u9175\u7d20\u304c\u6c88\u3093\u3067\u3044\u304f\u969b\u306bDNA\u3092\u542b\u3080\u53cd\u5fdc\u6db2\u3068\u591a\u5c11\u306f\u6df7\u3056\u3063\u305f\u308a\u3001\u6c88\u3093\u3060\u9175\u7d20\u3068\u63a5\u3059\u308b\u3068\u3053\u308d\u3067DNA\u3068\u53cd\u5fdc\u3057\u305f\u3053\u3068\u306a\u3069\u304c\u8003\u3048\u3089\u308c\u307e\u3057\u305f\u3002\u3057\u304b\u3057\u3001\u4e00\u756a\u4e0a\u306e1042 bp\u306e\u30d0\u30f3\u30c9\u306f\u5143\u306ePCR\u7523\u7269\u3067\u3059\u306e\u3067\u3001\u304b\u306a\u308a\u5207\u308c\u6b8b\u3063\u3066\u3044\u308b\u3068\u3044\u3046\u3053\u3068\u304c\u5206\u304b\u308a\u307e\u3059\u3002\u3084\u306f\u308a\u3001\u5236\u9650\u9175\u7d20\u51e6\u7406\u306e\u5834\u5408\u3067\u3082\u3001\u8a66\u85ac\u306e\u7dcf\u6db2\u91cf\u306e\u5c11\u306a\u304f\u3068\u3082\u534a\u91cf\u4ee5\u4e0a\u306e\u5bb9\u91cf\u3067\u3086\u3063\u304f\u308a\u4e01\u5be7\u306b\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u3059\u308b\u3053\u3068\u304c\u91cd\u8981\u3067\u3042\u308b\u3053\u3068\u3092\u304a\u793a\u3057\u3067\u304d\u307e\u3057\u305f\u3002<\/p>\n

\"\u56f32.

\u56f32. \u5236\u9650\u9175\u7d20\u51e6\u7406\u5f8c\u306e\u30a2\u30ac\u30ed\u30fc\u30b9\u30b2\u30eb\u96fb\u6c17\u6cf3\u52d5\u306e\u7d50\u679c
M. \u30b5\u30a4\u30ba\u30de\u30fc\u30ab\u30fc(1 kb Plus DNA Ladder)
1. Hind III\u306a\u3057\uff1a15 \u03bcL\u5bb9\u91cf\u3067\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0 5\u56de
2. Hind III\u3042\u308a\uff1a15 \u03bcL\u5bb9\u91cf\u3067\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0 5\u56de
3. Hind III\u3042\u308a\uff1a1 \u03bcL\u5bb9\u91cf\u3067\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0 5\u56de
4. Hind III\u3042\u308a\uff1a\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u3057\u306a\u3044<\/p><\/div>\n

\u307e\u3068\u3081<\/h2>\n

\u4eca\u56de\u306f\u3001\u5b9f\u969b\u306b\u5236\u9650\u9175\u7d20\u51e6\u7406\u3092\u5b9f\u65bd\u3057\u3001\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u3067\u306e\u6df7\u5408\u304c\u4e0d\u5341\u5206\u3060\u3068\u5b9f\u9a13\u7d50\u679c\u306b\u3069\u306e\u3088\u3046\u306a\u5f71\u97ff\u304c\u3042\u308b\u306e\u304b\u3092\u3084\u3063\u3066\u307f\u307e\u3057\u305f\u3002\u8a66\u85ac\u306a\u3069\u3092\u6df7\u5408\u3059\u308b\u969b\u306e\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u5bb9\u91cf\u306f\u3001\u5c11\u306a\u304f\u3068\u3082\u7dcf\u6db2\u91cf\u306e\u534a\u91cf\u4ee5\u4e0a\u3068\u3044\u3046\u3053\u3068\u3092\u610f\u8b58\u3057\u3066\u5b9f\u9a13\u306b\u53d6\u308a\u7d44\u3093\u3067\u3044\u305f\u3060\u304f\u3068\u3001\u3088\u308a\u6b63\u78ba\u306a\u5b9f\u9a13\u30c7\u30fc\u30bf\u3092\u5f97\u3066\u3044\u305f\u3060\u3051\u308b\u306e\u3067\u306f\u306a\u3044\u304b\u3068\u601d\u3044\u307e\u3059\u3002<\/p>\n

\u5f53\u793e\u306e\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u30c4\u30fc\u30eb\u306e\u9078\u629e\u3084\u5404\u30b5\u30f3\u30d7\u30eb\u306b\u9069\u3057\u305f\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u30c6\u30af\u30cb\u30c3\u30af\u3092\u3054\u89a7\u3044\u305f\u3060\u3051\u308b\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u30ac\u30a4\u30c9<\/b><\/p>\n

\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u306f\u3053\u3061\u3089<\/a><\/p>\n

\u30d4\u30da\u30c3\u30c8\u30b5\u30fc\u30d3\u30b9 \u304a\u554f\u3044\u5408\u308f\u305b\u30fb\u30ab\u30bf\u30ed\u30b0<\/b><\/p>\n

\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u306f\u3053\u3061\u3089<\/a><\/p>\n

\u95a2\u9023\u3059\u308b\u8a18\u4e8b
\n\u3010\u3084\u3063\u3066\u307f\u305f\u3011\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u3067\u8a66\u85ac\u3092\u3057\u3063\u304b\u308a\u6df7\u5408\u3059\u308b\u305f\u3063\u305f1\u3064\u306e\u30b3\u30c4\u3092\u52d5\u753b\u306b\u3057\u3066\u307f\u305f
\nhttps:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/tr-workshop-gsd-ts-1\/<\/a><\/p>\n

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