{"id":16245,"date":"2024-01-10T18:34:35","date_gmt":"2024-01-10T09:34:35","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/?p=16245"},"modified":"2026-02-18T18:14:57","modified_gmt":"2026-02-18T09:14:57","slug":"cell-culture-cell-separation-bid-ts-mbr-24001","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/cell-culture-cell-separation-bid-ts-mbr-24001\/","title":{"rendered":"\u30b7\u30f3\u30b0\u30eb\u30bb\u30eb\u89e3\u6790\u3084\u521d\u4ee3\u57f9\u990a\u306b\u4f7f\u308f\u308c\u308b\u7d30\u80de\u89e3\u96e2\u8a66\u85ac\u306e\u9078\u629e\u30ac\u30a4\u30c9"},"content":{"rendered":"<p>\u8fd1\u5e74\u306e\u6025\u6fc0\u306a\u6280\u8853\u9032\u6b69\u306b\u3088\u308b\u6b21\u4e16\u4ee3\u30b7\u30fc\u30b1\u30f3\u31f2\u306e\u30c7\u30fc\u30bf\u53d6\u5f97\u30b3\u30b9\u30c8\u306e\u4f4e\u4e0b\u306b\u3088\u308a\u3001\u30b7\u30f3\u30b0\u30eb\u30bb\u30ebRNA\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\uff08single-cell RNA-seq\u3001scRNA-seq\uff09\u306b\u4ee3\u8868\u3055\u308c\u308b\u30b7\u30f3\u30b0\u30eb\u30bb\u30eb\u89e3\u6790\u306f\u307e\u3059\u307e\u3059\u8eab\u8fd1\u306a\u5b9f\u9a13\u624b\u6cd5\u3068\u306a\u308a\u3064\u3064\u3042\u308a\u307e\u3059\u3002Google Scholar\u306b\u3088\u308b\u3068\u3001\u201dsingle-cell RNA-seq\u201d\u306e\u30ad\u30fc\u30ef\u30fc\u30c9\u3067\u30d2\u30c3\u30c8\u3059\u308b\u7d043\u4e07\u4ef6\u306e\u6587\u732e\u306e\u3046\u30612\u4e07\u4ef6\u306f2022\u5e74\u4ee5\u964d\u306b\u767a\u8868\u3055\u308c\u307e\u3057\u305f\u3002\u540c\u3058\u304f2022\u5e74\u4ee5\u964d\u306b\u767a\u8868\u3055\u308c\u305f\u6587\u732e\u306e\u691c\u7d22\u3067\u3001\u201dreal-time PCR\u201d\u304c3\u4e077000\u4ef6\u3001\u201dgenome editing\u201d\u304c2\u4e073000\u4ef6\u3001\u201dwestern blot\u201d\u304c2\u4e072000\u4ef6\u306e\u30d2\u30c3\u30c8\u3067\u3042\u308b\u3053\u3068\u304b\u3089\u3001scRNA-seq\u306e\u666e\u53ca\u304c\u3053\u308c\u3089\u306e\u4ee3\u8868\u7684\u306a\u5b9f\u9a13\u624b\u6cd5\u306b\u8feb\u308b\u52e2\u3044\u3067\u3042\u308b\u3053\u3068\u304c\u4f3a\u3048\u307e\u3059\uff082023\u5e7411\u6708\u6642\u70b9\uff09\u3002<\/p>\n<p>\u6d6e\u904a\u6027\u306e\u7d30\u80de\u3084\u63a5\u7740\u6027\u306e\u57f9\u990a\u7d30\u80de\u3092\u89e3\u6790\u5bfe\u8c61\u3068\u3059\u308b\u5834\u5408\u306f\u3001scRNA-seq\u306e\u305f\u3081\u306e\u5358\u96e2\u7d30\u80de\u306e\u53d6\u5f97\u306f\u6bd4\u8f03\u7684\u5bb9\u6613\u3067\u3059\u3002\u4e00\u65b9\u3001\u751f\u4f53\u306e\u81d3\u5668\u3084\u7d44\u7e54\u3092\u89e3\u6790\u5bfe\u8c61\u3068\u3059\u308b\u5834\u5408\u306f\u3001\u9175\u7d20\u51e6\u7406\u306a\u3069\u306b\u3088\u3063\u3066\u7d30\u80de\u3092\u5358\u96e2\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u7d30\u80de\u3078\u306e\u30b9\u30c8\u30ec\u30b9\u3092\u6700\u5c0f\u9650\u306b\u6291\u3048\u306a\u304c\u3089\u81d3\u5668\u30fb\u7d44\u7e54\u3092\u5206\u6563\u3055\u305b\u308b\u5b9f\u9a13\u6761\u4ef6\u306f\u3001\u5bfe\u8c61\u306e\u52d5\u7269\u7a2e\u3001\u500b\u4f53\u306e\u6210\u719f\u5ea6\uff08\u751f\u80b2\u671f\u9593\u3084\u767a\u751f\u6bb5\u968e\uff09\u3001\u7d30\u80de\u306e\u7a2e\u985e\u306a\u3069\u306b\u3088\u3063\u3066\u6700\u9069\u5316\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u3001\u5e45\u5e83\u3044\u7d30\u80de\u7a2e\u306b\u5bfe\u5fdc\u3067\u304d\u308b\u7d30\u80de\u5358\u96e2\u30ad\u30c3\u30c8\u306e\u958b\u767a\u3084\u6a5f\u68b0\u306b\u3088\u308b\u81ea\u52d5\u5316\u306f\u5bb9\u6613\u3067\u306f\u306a\u3044\u3068\u8003\u3048\u3089\u308c\u3001\u73fe\u5728\u3067\u3082\u591a\u304f\u306e\u7814\u7a76\u8005\u304c\u3055\u307e\u3056\u307e\u306a\u8a66\u85ac\u3092\u99c6\u4f7f\u3057\u3066\u624b\u4f5c\u696d\u3067\u5358\u96e2\u7d30\u80de\u3092\u8abf\u88fd\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>\u305d\u3053\u3067\u5f53\u8a18\u4e8b\u3067\u306f\u3001RNA-seq\u306e\u305f\u3081\u306e\u5358\u96e2\u7d30\u80de\u8abf\u88fd\u3078\u306e\u5b9f\u7e3e\u306e\u3042\u308b\u88fd\u54c1\u3092\u4e2d\u5fc3\u306b\u3001\u5f53\u793e\u306e\u7d30\u80de\u89e3\u96e2\u8a66\u85ac\u3092\u307e\u3068\u3081\u307e\u3057\u305f\u3002\u5f53\u8a18\u4e8b\u3067\u6271\u3063\u3066\u3044\u308b\u8a66\u85ac\u306e\u6982\u8981\u3092\u88681\u306b\u6574\u7406\u3057\u307e\u3057\u305f\u3002\u5404\u8a66\u85ac\u3054\u3068\u306b\u3054\u7d39\u4ecb\u3057\u3066\u3044\u307e\u3059\u306e\u3067\u3001\u7279\u5b9a\u306e\u8a66\u85ac\u306b\u3054\u8208\u5473\u306e\u3042\u308b\u65b9\u306f\u3001\u5f53\u8a18\u4e8b\u306e\u8a72\u5f53\u90e8\u5206\u3078\u304a\u9032\u307f\u304f\u3060\u3055\u3044\u3002<\/p>\n<table width=\"633\">\n<caption>\u88681. \u7d30\u80de\u89e3\u96e2\u8a66\u85ac\u306e\u9078\u629e\u30ac\u30a4\u30c9<\/caption>\n<tbody>\n<tr bgcolor=\"#696969\">\n<td style=\"text-align: center\" width=\"137\"><span style=\"color: #ffffff\">\u8a66\u85ac<\/span><\/td>\n<td style=\"text-align: center\" width=\"220\"><span style=\"color: #ffffff\">\u6982\u8981<\/span><\/td>\n<td style=\"text-align: center\" width=\"303\"><span style=\"color: #ffffff\">\u5099\u8003<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"137\"><a href=\"#Trypsin\">Trypsin<\/a><\/td>\n<td width=\"220\">\u4ee3\u8868\u7684\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u5206\u89e3\u9175\u7d20<\/td>\n<td width=\"303\">\u4e3b\u306b\u30d6\u30bf\u3084\u30a6\u30b7\u306e\u81b5\u81d3\u304b\u3089\u62bd\u51fa\u3055\u308c\u308b\u3002<br \/>\n\u4e0d\u7d14\u7269\u3068\u3057\u3066Trypsin\u4ee5\u5916\u306e\u9175\u7d20\u3082\u542b\u307e\u308c\u308b\u3002<\/td>\n<\/tr>\n<tr>\n<td width=\"137\"><a href=\"#Trypsin-EDTA\">Trypsin-EDTA<\/a><\/td>\n<td width=\"220\">Trypsin\u3068EDTA\u306e\u6df7\u5408\u7269<\/td>\n<td width=\"303\">\u57f9\u990a\u7d30\u80de\u306e\u7d99\u4ee3\u3067\u6c4e\u7528\u3055\u308c\u3066\u3044\u308b\u3002<br \/>\n\u7d30\u80de\u306e\u7a2e\u985e\u306b\u3088\u3063\u3066\u306f\u3001\u81d3\u5668\u30fb\u7d44\u7e54\u304b\u3089\u306e<br \/>\n\u7d30\u80de\u5358\u96e2\u306b\u9069\u3055\u306a\u3044\u5834\u5408\u304c\u3042\u308b\u3002<\/td>\n<\/tr>\n<tr>\n<td width=\"137\"><a href=\"#TrypLE\">TrypLE<\/a><\/td>\n<td width=\"220\">Trypsin\u69d8\u7d44\u307f\u63db\u3048\u9175\u7d20\u3068EDTA\u306e\u6df7\u5408\u7269<\/td>\n<td width=\"303\">Trypsin-EDTA\u306e\u4ee3\u66ff\u3068\u3057\u3066\u4f7f\u7528\u3067\u304d\u308b\u3002<br \/>\n\u9ad8\u7d14\u5ea6\u30fb\u9ad8\u5b89\u5b9a\u6027\u3067\u5ba4\u6e29\u4fdd\u7ba1\u3067\u304d\u308b\u3002<br \/>\nAnimal Origin Free\u88fd\u54c1<\/td>\n<\/tr>\n<tr>\n<td width=\"137\"><a href=\"#Collagenase\">Collagenase<\/a><\/td>\n<td width=\"220\">\u7d30\u83cc\u7531\u6765\u306e\u30b3\u30e9\u30fc\u30b2\u30f3\u5206\u89e3\u9175\u7d20<\/td>\n<td width=\"303\">\u8907\u6570\u306e\u30d7\u30ed\u30c6\u30a2\u30fc\u30bc\u304c\u542b\u307e\u308c\u3066\u3044\u308b\u3002<br \/>\n\u9175\u7d20\u6d3b\u6027\u306e\u30d0\u30e9\u30f3\u30b9\u306b\u3088\u3063\u3066Type\u5206\u985e\u3055\u308c\u308b\u3002<\/td>\n<\/tr>\n<tr>\n<td width=\"137\"><a href=\"#Dispase\">Dispase<\/a><\/td>\n<td width=\"220\">\u7d30\u83cc\u7531\u6765\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u5206\u89e3\u9175\u7d20<\/td>\n<td width=\"303\">\u57fa\u5e95\u819c\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5206\u89e3\u306b\u512a\u308c\u3066\u3044\u308b\u3002<br \/>\n\u4f5c\u7528\u304c\u7a4f\u3084\u304b\u3067\u9577\u6642\u9593\u51e6\u7406\u3059\u308b\u3053\u3068\u3082\u3067\u304d\u308b\u3002<br \/>\nAnimal Origin Free\u88fd\u54c1<\/td>\n<\/tr>\n<tr>\n<td width=\"137\"><a href=\"#Enzyme-free\">Enzyme-free<\/a><\/td>\n<td width=\"220\">EDTA\u3092\u542b\u6709\u3059\u308b\u751f\u7406\u7684\u5869\u985e\u6eb6\u6db2<\/td>\n<td width=\"303\">\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u5207\u65ad\u3057\u306a\u3044\u305f\u3081\u89e3\u96e2\u4f5c\u7528\u306f\u5f31\u3044\u3002<br \/>\nAnimal Origin Free\u88fd\u54c1<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>\u25bc\u3053\u3093\u306a\u65b9\u306b\u304a\u3059\u3059\u3081\u3067\u3059\uff01<\/p>\n<ul>\n<li>\u30b7\u30f3\u30b0\u30eb\u30bb\u30eb\u89e3\u6790\u306e\u7d30\u80de\u5358\u96e2\u3092\u884c\u3046\u65b9<\/li>\n<li>\u521d\u4ee3\u57f9\u990a\u5b9f\u9a13\u306e\u7d30\u80de\u5358\u96e2\u3092\u884c\u3046\u65b9<\/li>\n<li>\u7d30\u80de\u57f9\u990a\u3092\u884c\u3046\u65b9<\/li>\n<\/ul>\n<h2>Trypsin<\/h2>\n<p>Trypsin\u306f100\u5e74\u4ee5\u4e0a\u524d\u304b\u3089\u7d30\u80de\u89e3\u96e2\u306b\u5229\u7528\u3055\u308c\u3066\u3044\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u5206\u89e3\u9175\u7d20\u3067\u3059\uff08\u95a2\u9023\u8a18\u4e8b\u300c<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/cell_culture_history_1_bid_ts_1\/?CID=bid_clb_r04_jp_cp0000_pjt0000_bid00000_0so_blg_cell_culture_cell_separation_bid_ts_mbr_24001_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u7d30\u80de\u306e\u57f9\u990a\u3068\u7d99\u4ee3\u306e\u30eb\u30fc\u30c4\u3092\u63a2\u3063\u3066\u307f\u305f\u4ef6\uff08\u524d\u7de8\uff09<\/a>\u300d\uff09\u3002\u30a2\u30eb\u30ae\u30cb\u30f3\u3068\u30ea\u30b7\u30f3\u306e\u30ab\u30eb\u30dc\u30cb\u30eb\u5074\u306e\u30da\u30d7\u30c1\u30c9\u7d50\u5408\u3092\u9078\u629e\u7684\u306b\u5207\u65ad\u3059\u308b\u30a8\u30f3\u30c9\u30da\u30d7\u30c1\u30c0\u30fc\u30bc\u3067\u3001\u7d30\u80de\u9593\u3084\u7d30\u80de\u3068\u57fa\u8cea\u9593\u306e\u7d50\u5408\u3092\u62c5\u3046\u7d30\u80de\u8868\u9762\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u5207\u65ad\u3059\u308b\u3053\u3068\u306b\u3088\u3063\u3066\u7d30\u80de\u3092\u89e3\u96e2\u3055\u305b\u307e\u3059\u3002\u5f53\u793e\u306eGibco\u2122 Trypsin\u88fd\u54c1\u306b\u306f\u3001\u4efb\u610f\u306e\u6fc3\u5ea6\u306b\u6eb6\u89e3\u3057\u3066\u4f7f\u7528\u3059\u308b\u7c89\u672b\u30bf\u30a4\u30d7\u30680.25%\u307e\u305f\u306f2.5%\u306b\u8abf\u88fd\u3055\u308c\u305f\u6eb6\u6db2\u30bf\u30a4\u30d7\u304c\u3042\u308a\u307e\u3059\u30020.25%\u6eb6\u6db2\u306e\u6eb6\u5a92\u306fCa<sup>2+<\/sup>\/Mg<sup>2+<\/sup>\u30d5\u30ea\u30fc\u306eHBSS\u3067\u3001pH\u6307\u793a\u85ac\u306ephenol red\u304c\u542b\u307e\u308c\u3066\u3044\u307e\u3059\u304c\u30012.5%\u6eb6\u6db2\u306e\u6eb6\u5a92\u306f0.85%\u306eNaCl\u6c34\u6eb6\u6db2\uff08\u751f\u7406\u98df\u5869\u6c34\uff09\u3067\u3059\u3002\u3088\u304f\u77e5\u3089\u308c\u3066\u3044\u308b\u3088\u3046\u306b\u30bf\u30f3\u30d1\u30af\u8cea\u5206\u89e3\u9175\u7d20\u3067\u3042\u308bTrypsin\u81ea\u4f53\u304c\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u305f\u3081\u3001\u4fdd\u7ba1\u72b6\u6cc1\u306b\u3088\u3063\u3066\u306f\u81ea\u5df1\u6d88\u5316\u3057\u3066\u5f90\u3005\u306b\u6d3b\u6027\u304c\u4f4e\u4e0b\u3057\u307e\u3059\u3002\u5b9f\u9a13\u6761\u4ef6\u304c\u56fa\u307e\u308a\u6b21\u7b2c\u30011\u56de\u4f7f\u7528\u5206\u306e\u6db2\u91cf\u3067\u5c0f\u5206\u3051\u3057\u3066\u51b7\u51cd\u3057\u3001\u51cd\u7d50\u878d\u89e3\u3092\u7e70\u308a\u8fd4\u3055\u306a\u3044\u3088\u3046\u306b\u7ba1\u7406\u3059\u308b\u3053\u3068\u304c\u63a8\u5968\u3055\u308c\u307e\u3059\u3002\u3053\u308c\u306fTrypsin\u306b\u9650\u3089\u305a\u3001\u51b7\u51cd\u4fdd\u5b58\u304c\u53ef\u80fd\u306a\u8a66\u85ac\u5168\u822c\u306b\u9069\u7528\u3067\u304d\u308b\u7ba1\u7406\u65b9\u6cd5\u3067\u3059\u3002\u306a\u304a\u3001\u5f53\u793e\u88fd\u54c1\u306eTrypsin\u306f\u30d6\u30bf\u81b5\u81d3\u7531\u6765\u3067\u3059\u3002\u88682\u306b\u5f53\u793e\u306eTrypsin\u88fd\u54c1\u3092\u307e\u3068\u3081\u307e\u3057\u305f\u3002<\/p>\n<table width=\"537\">\n<caption>\u88682. Trypsin\u88fd\u54c1\u306e\u9078\u629e\u30ac\u30a4\u30c9<\/caption>\n<tbody>\n<tr bgcolor=\"#696969\">\n<td style=\"text-align: center\" width=\"81\"><span style=\"color: #ffffff\">\u88fd\u54c1\u756a\u53f7<\/span><\/td>\n<td style=\"text-align: center\" width=\"96\"><span style=\"color: #ffffff\">Volume<\/span><\/td>\n<td style=\"text-align: center\" width=\"87\"><span style=\"color: #ffffff\">Trypsin<\/span><\/td>\n<td style=\"text-align: center\" width=\"83\"><span style=\"color: #ffffff\">Phenol<\/span><br \/>\n<span style=\"color: #ffffff\">Red<\/span><\/td>\n<td style=\"text-align: center\" width=\"189\"><span style=\"color: #ffffff\">Buffer<\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"81\">27250018<\/td>\n<td style=\"text-align: center\" width=\"96\">100 g<\/td>\n<td style=\"text-align: center\" width=\"87\">Powder<\/td>\n<td style=\"text-align: center\" width=\"83\">X<\/td>\n<td style=\"text-align: center\" width=\"189\">X<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"81\">15050065<\/td>\n<td style=\"text-align: center\" width=\"96\">100 mL<\/td>\n<td style=\"text-align: center\" rowspan=\"2\" width=\"87\">0.25\uff05<\/td>\n<td style=\"text-align: center\" rowspan=\"2\" width=\"83\">\u3007<\/td>\n<td style=\"text-align: center\" rowspan=\"2\" width=\"189\">HBSS w\/o Ca<sup>2+<\/sup> &amp; Mg<sup>2+<\/sup><\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"81\">15050057<\/td>\n<td style=\"text-align: center\" width=\"96\">500 mL<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"81\">15090046<\/td>\n<td style=\"text-align: center\" width=\"96\">100 mL<\/td>\n<td style=\"text-align: center\" width=\"87\">2.5\uff05<\/td>\n<td style=\"text-align: center\" width=\"83\">X<\/td>\n<td style=\"text-align: center\" width=\"189\">0.85% NaCl<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h2>Trypsin-EDTA<\/h2>\n<p>Trypsin-EDTA\u306f\u30bf\u30f3\u30d1\u30af\u8cea\u5206\u89e3\u9175\u7d20\u306eTrypsin\u3068Ca<sup>2+<\/sup>\u3084Mg<sup>2+<\/sup>\u306a\u3069\u306e2\u4fa1\u306e\u967d\u30a4\u30aa\u30f3\u3092\u30ad\u30ec\u30fc\u30c8\u3059\u308b\u30a8\u30c1\u30ec\u30f3\u30b8\u30a2\u30df\u30f3\u56db\u9162\u9178\uff08ethylenediaminetetraacetic acid\uff09\u306e\u6df7\u5408\u6eb6\u6db2\u3067\u3059\u3002Ca<sup>2+<\/sup>\u3068Mg<sup>2+<\/sup>\u306f\u7d30\u80de\u9593\u3084\u7d30\u80de-\u57fa\u8cea\u9593\u306e\u63a5\u7740\u306b\u5fc5\u8981\u3067\u3042\u308b\u3053\u3068\u304b\u3089\u3001\u3053\u308c\u3089\u306e\u30a4\u30aa\u30f3\u3092\u30ad\u30ec\u30fc\u30c8\u3059\u308b\u3053\u3068\u306b\u3088\u3063\u3066\u7d30\u80de\u89e3\u96e2\u3092\u4fc3\u9032\u3057\u307e\u3059\u3002Trypsin-EDTA\u306f1950\u5e74\u4ee3\u306b\u57f9\u990a\u7d30\u80de\u306e\u89e3\u96e2\u306b\u6709\u7528\u3067\u3042\u308b\u3053\u3068\u304c\u5831\u544a\u3055\u308c\u307e\u3057\u305f\uff08\u95a2\u9023\u8a18\u4e8b\u300c<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/cell_culture_history_2_bid_ts_2\/?CID=bid_clb_r04_jp_cp0000_pjt0000_bid00000_0so_blg_cell_culture_cell_separation_bid_ts_mbr_24001_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u7d30\u80de\u306e\u57f9\u990a\u3068\u7d99\u4ee3\u306e\u30eb\u30fc\u30c4\u3092\u63a2\u3063\u3066\u307f\u305f\u4ef6\uff08\u5f8c\u7de8\uff09<\/a>\u300d\uff09\u3002\u305d\u308c\u4ee5\u6765\u3001\u4e3b\u306b\u63a5\u7740\u57f9\u990a\u7d30\u80de\u306e\u7d99\u4ee3\u6642\u306b\u4f7f\u7528\u3055\u308c\u308b\u8a66\u85ac\u3067\u3059\u304c\u3001\u81d3\u5668\u30fb\u7d44\u7e54\u306e\u7d30\u80de\u5206\u6563\u306b\u4f7f\u7528\u3055\u308c\u308b\u3053\u3068\u3082\u3042\u308a\u307e\u3059\u3002\u305f\u3060\u3001\u7d30\u80de\u306e\u7a2e\u985e\u306b\u3088\u3063\u3066\u306fEDTA\u304c\u7d30\u80de\u306b\u30c0\u30e1\u30fc\u30b8\u3092\u4e0e\u3048\u308b\u30b1\u30fc\u30b9\u304c\u3042\u308b\u3088\u3046\u3067\u3059\u3002EDTA\u4e0d\u542b\u306eTrypsin\u6eb6\u6db2\u3067\u78ba\u7acb\u3055\u308c\u305f\u7d30\u80de\u89e3\u96e2\u64cd\u4f5c\u3067\u306fTrypsin-EDTA\u3067\u7f6e\u304d\u63db\u3048\u3067\u304d\u306a\u3044\u3053\u3068\u304c\u3042\u308b\u305f\u3081\u6ce8\u610f\u304c\u5fc5\u8981\u3067\u3059\u3002\u3053\u306e\u3053\u3068\u306f\u3001\u5f8c\u8ff0\u3059\u308bTrypLE\u306b\u3082\u5f53\u3066\u306f\u307e\u308a\u307e\u3059\u3002\u5f53\u793e\u306eGibco\u2122 Trypsin-EDTA\u88fd\u54c1\u3092\u88683\u306b\u307e\u3068\u3081\u307e\u3057\u305f\u3002<\/p>\n<table width=\"633\">\n<caption>\u88683. Trypsin-EDTA\u88fd\u54c1\u306e\u9078\u629e\u30ac\u30a4\u30c9<\/caption>\n<tbody>\n<tr bgcolor=\"#696969\">\n<td style=\"text-align: center\" width=\"85\"><span style=\"color: #ffffff\">\u88fd\u54c1\u756a\u53f7<\/span><\/td>\n<td style=\"text-align: center\" width=\"123\"><span style=\"color: #ffffff\">Volume<\/span><\/td>\n<td style=\"text-align: center\" width=\"85\"><span style=\"color: #ffffff\">Trypsin<\/span><\/td>\n<td style=\"text-align: center\" width=\"79\"><span style=\"color: #ffffff\">EDTA<\/span><\/td>\n<td style=\"text-align: center\" width=\"83\"><span style=\"color: #ffffff\">Phenol<\/span><br \/>\n<span style=\"color: #ffffff\">Red<\/span><\/td>\n<td style=\"text-align: center\" width=\"179\"><span style=\"color: #ffffff\">Buffer<\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"85\">25300054<\/td>\n<td style=\"text-align: center\" width=\"123\">100 mL<\/td>\n<td style=\"text-align: center\" rowspan=\"3\" width=\"85\">0.05\uff05<\/td>\n<td style=\"text-align: center\" rowspan=\"3\" width=\"79\">0.48 mM<\/td>\n<td style=\"text-align: center\" rowspan=\"3\" width=\"83\">\u25cb<\/td>\n<td style=\"text-align: center\" rowspan=\"3\" width=\"179\">HBSS w\/o Ca<sup>2+<\/sup> &amp; Mg<sup>2+<\/sup><\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"85\">25300062<\/td>\n<td style=\"text-align: center\" width=\"123\">500 mL<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"85\">25300120<\/td>\n<td style=\"text-align: center\" width=\"123\">20 x 100 mL<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"85\">25200056<\/td>\n<td style=\"text-align: center\" width=\"123\">100 mL<\/td>\n<td style=\"text-align: center\" rowspan=\"3\" width=\"85\">0.25\uff05<\/td>\n<td style=\"text-align: center\" rowspan=\"3\" width=\"79\">0.91 mM<\/td>\n<td style=\"text-align: center\" rowspan=\"3\" width=\"83\">\u25cb<\/td>\n<td style=\"text-align: center\" rowspan=\"3\" width=\"179\">HBSS w\/o Ca<sup>2+<\/sup> &amp; Mg<sup>2+<\/sup><\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"85\">25200072<\/td>\n<td style=\"text-align: center\" width=\"123\">500 mL<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"85\">25200114<\/td>\n<td style=\"text-align: center\" width=\"123\">20 x 100 mL<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"85\">15400054<\/td>\n<td style=\"text-align: center\" width=\"123\">100 mL<\/td>\n<td style=\"text-align: center\" width=\"85\">0.5\uff05<\/td>\n<td style=\"text-align: center\" width=\"79\">4.8 mM<\/td>\n<td style=\"text-align: center\" width=\"83\">X<\/td>\n<td style=\"text-align: center\" width=\"179\">0.85% NaCl<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"85\">R001100<\/td>\n<td style=\"text-align: center\" width=\"123\">100 mL<\/td>\n<td style=\"text-align: center\" width=\"85\">0.025\uff05<\/td>\n<td style=\"text-align: center\" width=\"79\">0.3 mM<\/td>\n<td style=\"text-align: center\" width=\"83\">\u25cb<\/td>\n<td style=\"text-align: center\" width=\"179\">\u203b<\/td>\n<\/tr>\n<\/tbody>\n<tfoot>\n<tr>\n<td style=\"border-bottom: none\" colspan=\"6\" width=\"633\">\u203b\u7d44\u6210\u306f<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/technical-resources\/media-formulation.338.html?CID=bid_clb_r04_jp_cp0000_pjt0000_bid00000_0so_blg_cell_culture_cell_separation_bid_ts_mbr_24001_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3053\u3061\u3089<\/a>\u3092\u3054\u78ba\u8a8d\u304f\u3060\u3055\u3044\u3002<\/td>\n<\/tr>\n<\/tfoot>\n<\/table>\n<h2>TrypLE\u30b7\u30ea\u30fc\u30ba<\/h2>\n<p>Gibco\u2122 <a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/cell-culture\/mammalian-cell-culture\/reagents\/trypsin\/tryple-express.html?CID=bid_clb_r04_jp_cp0000_pjt0000_bid00000_0so_blg_cell_culture_cell_separation_bid_ts_mbr_24001_Social_LAB\" target=\"_blank\" rel=\"noopener\">TrypLE\u2122\u30b7\u30ea\u30fc\u30ba<\/a>\u306fTrypsin-EDTA\u6eb6\u6db2\u306e\u4ee3\u66ff\u54c1\u3068\u3057\u3066\u4f7f\u7528\u3067\u304d\u308b\u7d30\u80de\u89e3\u96e2\u9175\u7d20\u8a66\u85ac\u3067\u3059\u3002\u5fae\u751f\u7269\u306b\u7523\u751f\u3055\u305b\u305fTrypsin\u69d8\u306e\u7d44\u63db\u3048\u9175\u7d20\u3092\u9ad8\u7d14\u5ea6\u3067\u7cbe\u88fd\u3057\u3066\u88fd\u9020\u3057\u3066\u3044\u308b\u305f\u3081\u3001\u81b5\u81d3\u7531\u6765\u306e\u4e0d\u7d14\u7269\u306e\u9175\u7d20\u304c\u6b8b\u5b58\u3057\u3066\u3044\u308bTrypsin\u3068\u6bd4\u3079\u3066\u7d30\u80de\u306b\u3084\u3055\u3057\u3044\u3068\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u52d5\u7269\u7531\u6765\u6210\u5206\u3092\u542b\u307e\u306a\u3044\u88fd\u54c1\uff08Animal Origin Free\u3001AOF\uff09\u3067\u3042\u308a\u3001TrypLE\u6eb6\u6db2\u3068\u540c\u91cf\u4ee5\u4e0a\u306e\u3001\u7121\u8840\u6e05\u57f9\u5730\uff08\u8840\u6e05\u5165\u308a\u57f9\u5730\u3067\u3082\u53ef\uff09\u3084\u751f\u7406\u7684\u5869\u985e\u6eb6\u6db2\u3067\u5e0c\u91c8\u3059\u308b\u3053\u3068\u3067\u53cd\u5fdc\u3092\u6b62\u3081\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u305f\u3081\u3001\u7121\u8840\u6e05\u57f9\u5730\u3092\u7528\u3044\u305f\u7d30\u80de\u57f9\u990a\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306b\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u307e\u305f\u3001TrypLE\u306f\u5e38\u6e29\u3067\u4fdd\u7ba1\u3067\u304d\u308b\u305f\u3081\u53d6\u6271\u3044\u306e\u9762\u3067\u3082\u4fbf\u5229\u3067\u3059\uff08\u51b7\u8535\u30fb\u51b7\u51cd\u4fdd\u7ba1\u3082\u53ef\uff09\u3002<\/p>\n<p>TrypLE\u30b7\u30ea\u30fc\u30ba\u306b\u306f\u9175\u7d20\u6fc3\u5ea6\u306e\u9055\u3044\uff081X\u307e\u305f\u306f10X\uff09\u3084Phenol Red\u306e\u6709\u7121\u306b\u52a0\u3048\u3066\u300cExpress\u300d\u300cSelect\u300d\u300cCTS Select\u300d\u306e3\u7a2e\u985e\u306e\u5206\u985e\u304c\u3042\u308a\u307e\u3059\u3002\u3053\u308c\u3089\u306e3\u7a2e\u985e\u306b\u542b\u307e\u308c\u308bTrypLE\u306e\u9175\u7d20\u3068\u3057\u3066\u306e\u6027\u80fd\u306f\u540c\u7b49\u3067\u3059\u304c\u3001\u76ee\u7684\u306b\u3088\u3063\u3066\u4f7f\u3044\u5206\u3051\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<ul>\n<li>Gibco\u2122 TrypLE\u2122 Express Enzyme\uff1a\u7814\u7a76\u7528\uff08Research Use Only\u3001RUO\uff09\u306e\u6a19\u6e96\u30b0\u30ec\u30fc\u30c9<\/li>\n<li>Gibco\u2122 TrypLE\u2122 Select Enzyme\uff1a\u7814\u7a76\u7528\u30fb\u5de5\u696d\u539f\u6599\u7528\u306e\u30b0\u30ec\u30fc\u30c9<\/li>\n<li>Gibco\u2122 CTS\u2122 TrypLE Select Enzyme\uff1a\u518d\u751f\u533b\u7642\u7814\u7a76\u30fb\u81e8\u5e8a\u7814\u7a76\u306a\u3069\u306e\u305f\u3081\u306e\u30b0\u30ec\u30fc\u30c9<\/li>\n<\/ul>\n<p>\u4e00\u822c\u7684\u306a\u7814\u7a76\u7528\u9014\u306e\u5834\u5408\u306fTrypLE Express Enzyme\u3092\u304a\u4f7f\u3044\u304f\u3060\u3055\u3044\u3002\u88fd\u9020\u306a\u3069\u3092\u8996\u91ce\u306b\u5165\u308c\u3066\u3044\u308b\u5834\u5408\u306f\u3001\u6700\u5148\u7aef\u306e\u65bd\u8a2d\u306b\u304a\u3044\u3066\u5c02\u7528\u306e\u88c5\u7f6e\u3067\u88fd\u9020\u3055\u308c\u3066\u3044\u308bTrypLE Select Enzyme\u304c\u63a8\u5968\u3057\u307e\u3059\u3002CTS TrypLE Select Enzyme\u306e\u300cCTS\u300d\u306fCell Therapy Systems\u306e\u7565\u8a9e\u3067\u3001\u57fa\u790e\u7814\u7a76\u304b\u3089\u81e8\u5e8a\u7814\u7a76\u307e\u3067\u3092\u30b5\u30dd\u30fc\u30c8\u3059\u308b\u305f\u3081\u306e\u88fd\u54c1\u30b7\u30ea\u30fc\u30ba\u3067\u3059\u3002CTS\u30b7\u30ea\u30fc\u30ba\u306e\u7279\u5fb4\u3084\u88fd\u54c1\u306b\u3064\u3044\u3066\u306f<a href=\"https:\/\/assets.thermofisher.com\/TFS-Assets\/BID\/brochures\/gibco-cts-brochure-ja.pdf?CID=bid_clb_r04_jp_cp0000_pjt0000_bid00000_0so_blg_cell_culture_cell_separation_bid_ts_mbr_24001_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3053\u3061\u3089<\/a>\u306e\u8cc7\u6599\u3092\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002\u306a\u304aCTS\u30b7\u30ea\u30fc\u30ba\u306e\u88fd\u54c1\u306f\u300c\u518d\u751f\u533b\u7642\u7b49\u88fd\u54c1\u6750\u6599\u9069\u683c\u6027\u78ba\u8a8d\u66f8\u300d\u304c\u53d6\u5f97\u3055\u308c\u3066\u3044\u307e\u3059\uff08\u8a72\u5f53\u88fd\u54c1\u4e00\u89a7\u3068\u554f\u3044\u5408\u308f\u305b\u30d5\u30a9\u30fc\u30e0\u306f<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/sbi-cert-inquiry.html?CID=bid_clb_r04_jp_cp0000_pjt0000_bid00000_0so_blg_cell_culture_cell_separation_bid_ts_mbr_24001_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3053\u3061\u3089<\/a>\uff09\u3002\u88684\u3067TrypLE\u88fd\u54c1\u3092\u6574\u7406\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<table width=\"633\">\n<caption>\u88684. TrypLE\u88fd\u54c1\u306e\u9078\u629e\u30ac\u30a4\u30c9<\/caption>\n<tbody>\n<tr bgcolor=\"#696969\">\n<td style=\"text-align: center\" width=\"85\"><span style=\"color: #ffffff\">\u88fd\u54c1\u756a\u53f7<\/span><\/td>\n<td style=\"text-align: center\" width=\"102\"><span style=\"color: #ffffff\">Volume<\/span><\/td>\n<td style=\"text-align: center\" width=\"138\"><span style=\"color: #ffffff\">\u88fd\u54c1\u540d<\/span><\/td>\n<td style=\"text-align: center\" width=\"113\"><span style=\"color: #ffffff\">TrypLE<br \/>\nConcentration<\/span><\/td>\n<td style=\"text-align: center\" width=\"72\"><span style=\"color: #ffffff\">EDTA<\/span><\/td>\n<td style=\"text-align: center\" width=\"74\"><span style=\"color: #ffffff\">Phenol<br \/>\nRed<\/span><\/td>\n<td style=\"text-align: center\" width=\"49\"><span style=\"color: #ffffff\">Buffer<\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"85\">12604013<\/td>\n<td style=\"text-align: center\" width=\"102\">100 mL<\/td>\n<td style=\"text-align: center\" rowspan=\"4\" width=\"138\">TrypLE Express Enzyme<\/td>\n<td style=\"text-align: center\" rowspan=\"4\" width=\"113\">1X<\/td>\n<td style=\"text-align: center\" rowspan=\"4\" width=\"72\">1.1 mM<\/td>\n<td style=\"text-align: center\" rowspan=\"4\" width=\"74\">X<\/td>\n<td style=\"text-align: center\" rowspan=\"4\" width=\"49\">DPBS<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"85\">12604021<\/td>\n<td style=\"text-align: center\" width=\"102\">500 mL<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"85\">12604039<\/td>\n<td style=\"text-align: center\" width=\"102\">20 x 100 mL<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"85\">12604054<\/td>\n<td style=\"text-align: center\" width=\"102\">5000 mL<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"85\">12605010<\/td>\n<td style=\"text-align: center\" width=\"102\">100 mL<\/td>\n<td style=\"text-align: center\" rowspan=\"4\" width=\"138\">TrypLE Express Enzyme<\/td>\n<td style=\"text-align: center\" rowspan=\"4\" width=\"113\">1X<\/td>\n<td style=\"text-align: center\" rowspan=\"4\" width=\"72\">1.1 mM<\/td>\n<td style=\"text-align: center\" rowspan=\"4\" width=\"74\">\u25cb<\/td>\n<td style=\"text-align: center\" rowspan=\"4\" width=\"49\">DPBS<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"85\">12605028<\/td>\n<td style=\"text-align: center\" width=\"102\">500 mL<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"85\">12605036<\/td>\n<td style=\"text-align: center\" width=\"102\">20 x 100 mL<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"85\">12605093<\/td>\n<td style=\"text-align: center\" width=\"102\">5000<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"85\">12563011<\/td>\n<td style=\"text-align: center\" width=\"102\">100<\/td>\n<td style=\"text-align: center\" rowspan=\"2\" width=\"138\">TrypLE Select Enzyme<\/td>\n<td style=\"text-align: center\" rowspan=\"2\" width=\"113\">1X<\/td>\n<td style=\"text-align: center\" rowspan=\"2\" width=\"72\">1.1 mM<\/td>\n<td style=\"text-align: center\" rowspan=\"2\" width=\"74\">X<\/td>\n<td style=\"text-align: center\" rowspan=\"2\" width=\"49\">DPBS<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"85\">12563029<\/td>\n<td style=\"text-align: center\" width=\"102\">500<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"85\">A1217701<\/td>\n<td style=\"text-align: center\" width=\"102\">100<\/td>\n<td style=\"text-align: center\" rowspan=\"3\" width=\"138\">TrypLE Select Enzyme<\/td>\n<td style=\"text-align: center\" rowspan=\"3\" width=\"113\"><span style=\"color: #ff0000\">10X<\/span><\/td>\n<td style=\"text-align: center\" rowspan=\"3\" width=\"72\">1.1 mM<\/td>\n<td style=\"text-align: center\" rowspan=\"3\" width=\"74\">X<\/td>\n<td style=\"text-align: center\" rowspan=\"3\" width=\"49\">DPBS<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"85\">A1217702<\/td>\n<td style=\"text-align: center\" width=\"102\">500<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"85\">A1217703<\/td>\n<td style=\"text-align: center\" width=\"102\">20 x 100 mL<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"85\">A1285901<\/td>\n<td style=\"text-align: center\" width=\"102\">100<\/td>\n<td style=\"text-align: center\" rowspan=\"2\" width=\"138\"><span style=\"color: #ff0000\">CTS<\/span> TrypLE Select Enzyme<\/td>\n<td style=\"text-align: center\" rowspan=\"2\" width=\"113\">1X<\/td>\n<td style=\"text-align: center\" rowspan=\"2\" width=\"72\">1.1 mM<\/td>\n<td style=\"text-align: center\" rowspan=\"2\" width=\"74\">X<\/td>\n<td style=\"text-align: center\" rowspan=\"2\" width=\"49\">DPBS<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"85\">A4738001<\/td>\n<td style=\"text-align: center\" width=\"102\">1000<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>\u201c10X\u201d\u306eTrypLE Select Enzyme\u306f\u63a5\u7740\u304c\u5f37\u56fa\u306a\u7d30\u80de\u306e\u5206\u6563\u306b\u305d\u306e\u307e\u307e\u4f7f\u7528\u3067\u304d\u308b\u3088\u3046\u306b\u3001EDTA\u306e\u6fc3\u5ea6\u3084DPBS\u306e\u7d44\u6210\u306f\u201d1X\u201d\u3068\u540c\u7b49\u3067\u3059\u3002\u3057\u305f\u304c\u3063\u3066\u3001\u4efb\u610f\u306e\u6fc3\u5ea6\u306b\u5e0c\u91c8\u3057\u3066\u4f7f\u7528\u3059\u308b\u5834\u5408\u306f\u3001\u9069\u5207\u306a\u6fc3\u5ea6\u306eEDTA\u3092\u542b\u3080DPBS\u3092\u7528\u3044\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u8a73\u3057\u3044\u624b\u9806\u306f<a href=\"https:\/\/tools.thermofisher.com\/content\/sfs\/manuals\/TrypLE_man_JP.pdf?CID=bid_clb_r04_jp_cp0000_pjt0000_bid00000_0so_blg_cell_culture_cell_separation_bid_ts_mbr_24001_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u88fd\u54c1\u30de\u30cb\u30e5\u30a2\u30eb<\/a>\u306b\u8a18\u8f09\u3055\u308c\u3066\u3044\u307e\u3059\u306e\u3067\u3054\u4f7f\u7528\u306e\u969b\u306f\u3054\u78ba\u8a8d\u304f\u3060\u3055\u3044\u3002<\/p>\n<h2>Collagenase<\/h2>\n<p>\u591a\u7d30\u80de\u751f\u7269\u306e\u7d44\u7e54\u306f\u30b3\u30e9\u30fc\u30b2\u30f3\u3001\u30d5\u30a3\u30d6\u30ed\u30cd\u30af\u30c1\u30f3\u3001\u30e9\u30df\u30cb\u30f3\u306a\u3069\u306e\u7d30\u80de\u5916\u57fa\u8cea\uff08ECM\uff09\u3092\u8c4a\u5bcc\u306b\u542b\u3093\u3067\u3044\u307e\u3059\u3002Collagenase\u306f\u7d44\u7e54\u4e2d\u306b\u6700\u3082\u591a\u304f\u5b58\u5728\u3059\u308bECM\u3067\u3042\u308b\u30b3\u30e9\u30fc\u30b2\u30f3\u3092\u5206\u89e3\u3059\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u5206\u89e3\u9175\u7d20\u306e\u7dcf\u79f0\u3067\u3059\u3002\u5f53\u793e\u304c\u8ca9\u58f2\u3057\u3066\u3044\u308bGibco\u2122 Collagenase\u306f\u30d2\u30b9\u30c8\u30ea\u30c1\u30af\u30e0\u83cc\uff08Clostridium histolyticum\uff09\u304b\u3089\u5206\u96e2\u7cbe\u88fd\u3055\u308c\u305f\u7d30\u83cc\u6027\u30b3\u30e9\u30b2\u30ca\u30fc\u30bc\u306e\u7c89\u672b\u88fd\u54c1\u3067\u3059\u3002\u3053\u306eCollagenase\u306f\u3001\u30b3\u30e9\u30fc\u30b2\u30f3\u5206\u5b50\u306b\u7e70\u308a\u8fd4\u3057\u51fa\u73fe\u3059\u308b\u300cProline-X\uff08\u4e2d\u6027\u30a2\u30df\u30ce\u9178\uff09-Glycine-Proline\u300d\u306e\u914d\u5217\u306eX\u3068Glycine\u306e\u9593\u3092\u5207\u65ad\u3057\u307e\u3059\u3002\u4e00\u822c\u306b\u4e09\u91cd\u3089\u305b\u3093\u69cb\u9020\u3092\u3082\u3064\u5929\u7136\u30b3\u30e9\u30fc\u30b2\u30f3\u7dda\u7dad\u306f\u751f\u7406\u7684\u6761\u4ef6\u4e0b\u306b\u304a\u3044\u3066Trypsin\u306a\u3069\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u5206\u89e3\u9175\u7d20\u306b\u8010\u6027\u3092\u793a\u3057\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u3001\u30b3\u30e9\u30fc\u30b2\u30f3\u3092\u8c4a\u5bcc\u306b\u542b\u3080\u7d44\u7e54\u304b\u3089\u306e\u7d30\u80de\u306e\u5358\u96e2\u306bCollagenase\u304c\u4f7f\u7528\u3055\u308c\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u88685\u306b\u5f53\u793e\u306eCollagenase\u88fd\u54c1\u3092\u307e\u3068\u3081\u307e\u3057\u305f\u3002<\/p>\n<table width=\"783\">\n<caption>\u88685. Collagenase\u88fd\u54c1\u306e\u9078\u629e\u30ac\u30a4\u30c9<\/caption>\n<tbody>\n<tr bgcolor=\"#696969\">\n<td style=\"text-align: center\" width=\"81\"><span style=\"color: #ffffff\">\u88fd\u54c1\u756a\u53f7<\/span><\/td>\n<td style=\"text-align: center\" width=\"173\"><span style=\"color: #ffffff\">\u88fd\u54c1\u540d<\/span><\/td>\n<td style=\"text-align: center\" width=\"87\"><span style=\"color: #ffffff\">Size<\/span><\/td>\n<td style=\"text-align: center\" width=\"181\"><span style=\"color: #ffffff\">Tissue\/Cell type<\/span><\/td>\n<td style=\"text-align: center\" width=\"261\"><span style=\"color: #ffffff\">Memo<\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"81\">17100017<\/td>\n<td style=\"text-align: center\" width=\"173\">Collagenase, Type I<\/td>\n<td style=\"text-align: center\" width=\"87\">1 g<\/td>\n<td style=\"text-align: center\" rowspan=\"2\" width=\"181\">\u4e0a\u76ae\u3001\u526f\u814e\u3001\u80ba\u3001<br \/>\n\u8102\u80aa\u3001\u809d\u81d3<\/td>\n<td style=\"text-align: center\" rowspan=\"2\" width=\"261\">collagenase\u3001caseinase\u3001<br \/>\nclostripain\u3001trypsin\u306e<br \/>\n\u6d3b\u6027\u304c\u30d0\u30e9\u30f3\u30b9\u3088\u304f\u542b\u307e\u308c\u308b<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"81\">17018029<\/td>\n<td style=\"text-align: center\" width=\"173\">Collagenase, Type I<\/td>\n<td style=\"text-align: center\" width=\"87\">500 mg<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"81\">17101015<\/td>\n<td style=\"text-align: center\" width=\"173\">Collagenase, Type II<\/td>\n<td style=\"text-align: center\" width=\"87\">1 g<\/td>\n<td style=\"text-align: center\" width=\"181\">\u5fc3\u81d3\u3001\u7532\u72b6\u817a\u3001\u553e\u6db2\u817a\u3001\u809d\u81d3\u3001\u9aa8\u3001\u8edf\u9aa8<\/td>\n<td style=\"text-align: center\" width=\"261\">clostripain\u306e\u6d3b\u6027\u304c\u6bd4\u8f03\u7684\u9ad8\u3044<br \/>\nclostripain\u306fArg\u6b8b\u57fa\u306eC\u672b\u5074\u3092<br \/>\n\u5207\u65ad\u3059\u308b\u30a8\u30f3\u30c9\u30da\u30d7\u30c1\u30c0\u30fc\u30bc<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"81\">17104019<\/td>\n<td style=\"text-align: center\" width=\"173\">Collagenase, Type IV<\/td>\n<td style=\"text-align: center\" width=\"87\">1 g<\/td>\n<td style=\"text-align: center\" width=\"181\">\u81b5\u5cf6<\/td>\n<td style=\"text-align: center\" width=\"261\">trypsin\u6d3b\u6027\u304c\u6bd4\u8f03\u7684\u4f4e\u3044<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>\u88685\u306eMemo\u6b04\u306b\u3042\u308b\u3088\u3046\u306b\u3001Collagenase\u88fd\u54c1\u306b\u306fcollagenase\u4ee5\u5916\u306bcaseinase\u3001clostripain\u3001trypsin\u306a\u3069\u306e\u6d3b\u6027\u304c\u542b\u307e\u308c\u3066\u3044\u307e\u3059\u3002\u3053\u308c\u3089\u306e\u6d3b\u6027\u306e\u30d0\u30e9\u30f3\u30b9\u306b\u3088\u3063\u3066Type I\u3001II\u3001IV\u306b\u5206\u985e\u3055\u308c\u3066\u304a\u308a\u3001\u305d\u308c\u305e\u308c\u306b\u9069\u3057\u305f\u81d3\u5668\u30fb\u7d44\u7e54\u306e\u7a2e\u985e\u304c\u77e5\u3089\u308c\u3066\u3044\u307e\u3059\u3002\u8868\u4e2d\u306e\u8a18\u8f09\u306f\u4ee3\u8868\u4f8b\u3067\u3059\u306e\u3067\u3001\u8a18\u8f09\u4ee5\u5916\u306e\u3055\u307e\u3056\u307e\u306a\u7d44\u7e54\u3078\u306e\u4f7f\u7528\u4e8b\u4f8b\u304c\u3042\u308a\u307e\u3059\u3002Collagenase\u88fd\u54c1\u306b\u542b\u307e\u308c\u308b\u8907\u6570\u306e\u9175\u7d20\u6d3b\u6027\u306f\u3001\u52b9\u7387\u7684\u306a\u7d30\u80de\u89e3\u96e2\u306b\u5bc4\u4e0e\u3059\u308b\u3068\u3068\u3082\u306b\u7d30\u80de\u3078\u306e\u30c0\u30e1\u30fc\u30b8\u306e\u539f\u56e0\u306b\u3082\u306a\u308a\u3048\u307e\u3059\u3002\u91cd\u91cf\u5f53\u305f\u308a\u306e\u9175\u7d20\u6d3b\u6027\u306f\u30ed\u30c3\u30c8\u3054\u3068\u306b\u7570\u306a\u308b\u305f\u3081\u3001\u5206\u6790\u8a3c\u660e\u66f8\u306b\u8a18\u8f09\u3055\u308c\u3066\u3044\u308b\u9175\u7d20\u6d3b\u6027\u3092\u53c2\u8003\u306b\u30e6\u30cb\u30c3\u30c8\u6fc3\u5ea6\uff08U\/mL\uff09\u3092\u8a2d\u5b9a\u3057\u3066\u6eb6\u89e3\u3059\u308b\u3068\u3068\u3082\u306b\u3001\u30ed\u30c3\u30c8\u5909\u66f4\u6642\u306b\u306f\u30ed\u30c3\u30c8\u30c1\u30a7\u30c3\u30af\u3092\u884c\u3046\u3053\u3068\u304c\u63a8\u5968\u3055\u308c\u307e\u3059\u3002\u306a\u304a\u3001\u5206\u6790\u8a3c\u660e\u66f8\u306e\u5165\u624b\u65b9\u6cd5\u306b\u3064\u3044\u3066\u306f\u8ffd\u3063\u3066\u89e6\u308c\u307e\u3059\u3002<\/p>\n<h2>Dispase<\/h2>\n<p>Gibco\u2122 <a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/17105041?CID=bid_clb_r04_jp_cp0000_pjt0000_bid00000_0so_blg_cell_culture_cell_separation_bid_ts_mbr_24001_Social_LAB\" target=\"_blank\" rel=\"noopener\">Dispase II<\/a>\u306f\u30b0\u30e9\u30e0\u967d\u6027\u7d30\u83cc\u306e1\u7a2e\u3067\u3042\u308bPaenibacillus polymyxa\u304c\u7523\u751f\u3059\u308b\u30a2\u30df\u30ce\u30a8\u30f3\u30c9\u30da\u30d7\u30c1\u30c0\u30fc\u30bc\u3067\u3059\u3002\u975e\u6975\u6027\u30a2\u30df\u30ce\u9178\u6b8b\u57fa\u306eN\u672b\u5074\u306e\u30da\u30d7\u30c1\u30c9\u7d50\u5408\u3092\u5207\u65ad\u3057\u307e\u3059\u3002\u9175\u7d20\u6d3b\u6027\u304c\u6bd4\u8f03\u7684\u7a4f\u3084\u304b\u3067\u7d30\u80de\u50b7\u5bb3\u304c\u5c11\u306a\u3044\u3001\u8840\u6e05\u5165\u308a\u57f9\u5730\u4e2d\u3067\u9175\u7d20\u6d3b\u6027\u3092\u767a\u63ee\u3059\u308b\u3001\u81ea\u8eab\u3092\u5206\u89e3\u3057\u306a\u3044\u305f\u3081\u9577\u6642\u9593\u306e\u53cd\u5fdc\u304c\u53ef\u80fd\u306a\u3069\u306e\u6709\u7528\u306a\u7279\u5fb4\u304c\u3042\u308a\u307e\u3059\u3002\u57fa\u5e95\u819c\u3092\u69cb\u6210\u3059\u308bECM\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u3088\u304f\u5206\u89e3\u3059\u308b\u305f\u3081\u3001\u9069\u5207\u306b\u51e6\u7406\u3059\u308b\u3068\u4e0a\u76ae\u7d44\u7e54\u3068\u9593\u8cea\u7d44\u7e54\u3092\u5206\u96e2\u3055\u305b\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\uff08\u52d5\u753b\uff09\u3002Collagenase\u3068\u540c\u69d8\u306b\u30ed\u30c3\u30c8\u3054\u3068\u306b\u9175\u7d20\u6d3b\u6027\u304c\u7570\u306a\u308b\u305f\u3081\u3001\u5206\u6790\u8a3c\u660e\u66f8\u3092\u78ba\u8a8d\u3057\u3066\u7c89\u672b\u3092\u6eb6\u89e3\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n<figure class=\"wp-block-video\">\n  <video controls=\"\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2024\/01\/cell-culture-cell-separation-bid-ts-mbr-24001-01.mp4\" playsinline width=\"400\" poster=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2024\/01\/cell-culture-cell-separation-bid-ts-mbr-24001-image.png\"><\/video><br \/>\n<\/figure>\n<h2 id=\"Enzyme-free\">\u9175\u7d20\u30d5\u30ea\u30fc\u306e\u7d30\u80de\u89e3\u96e2\u8a66\u85ac<\/h2>\n<p>\u4ee5\u4e0a\u306f\u3001\u3044\u305a\u308c\u3082\u30bf\u30f3\u30d1\u30af\u8cea\u5206\u89e3\u9175\u7d20\u306e\u6d3b\u6027\u306b\u3088\u3063\u3066\u7d30\u80de\u63a5\u7740\u3092\u5207\u65ad\u3057\u3066\u7d30\u80de\u3092\u89e3\u96e2\u3055\u305b\u308b\u88fd\u54c1\u3067\u3057\u305f\u3002\u6700\u5f8c\u306b\u3001EDTA\u306b\u3088\u308b\u30ad\u30ec\u30fc\u30c8\u4f5c\u7528\u306b\u3088\u3063\u3066\u7d30\u80de\u3092\u89e3\u96e2\u3055\u305b\u308b\u9175\u7d20\u30d5\u30ea\u30fc\u306e\u88fd\u54c1\u3092\u3054\u6848\u5185\u3057\u307e\u3059\u3002\u3053\u308c\u307e\u3067\u306e\u88fd\u54c1\u3068\u6bd4\u3079\u3066\u7d30\u80de\u89e3\u96e2\u4f5c\u7528\u304c\u5f31\u3044\u305f\u3081\u3001\u81d3\u5668\u30fb\u7d44\u7e54\u306e\u89e3\u96e2\u3084\u3001\u63a5\u7740\u306e\u5f37\u3044\u57f9\u990a\u7d30\u80de\u306e\u89e3\u96e2\u306b\u306f\u9069\u3057\u3066\u3044\u307e\u305b\u3093\u3002\u9006\u306b\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u5206\u89e3\u9175\u7d20\u3092\u542b\u307e\u306a\u3044\u305f\u3081\u3001\u7d30\u80de\u8868\u9762\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u50b7\u3064\u3051\u305a\u306b\u89e3\u6790\u3059\u308b\u5fc5\u8981\u304c\u3042\u308b\u5834\u5408\u306a\u3069\u306b\u6709\u7528\u3067\u3059\u3002\u5f53\u793e\u306e\u88fd\u54c1\u3092\u88686\u306b\u307e\u3068\u3081\u307e\u3057\u305f\u3002<\/p>\n<table width=\"723\">\n<caption>\u88686.\u9175\u7d20\u30d5\u30ea\u30fc\u306e\u7d30\u80de\u89e3\u96e2\u8a66\u85ac\u306e\u9078\u629e\u30ac\u30a4\u30c9<\/caption>\n<tbody>\n<tr bgcolor=\"#696969\">\n<td style=\"text-align: center\" width=\"89\"><span style=\"color: #ffffff\">\u88fd\u54c1\u756a\u53f7<\/span><\/td>\n<td style=\"text-align: center\" width=\"297\"><span style=\"color: #ffffff\">\u88fd\u54c1\u540d<\/span><\/td>\n<td style=\"text-align: center\" width=\"87\"><span style=\"color: #ffffff\">Volume<\/span><\/td>\n<td style=\"text-align: center\" width=\"91\"><span style=\"color: #ffffff\">EDTA<\/span><\/td>\n<td style=\"text-align: center\" width=\"159\"><span style=\"color: #ffffff\">Buffer<\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"89\">15040066<\/td>\n<td style=\"text-align: center\" width=\"297\">Gibco\u2122 Versene Solution<\/td>\n<td style=\"text-align: center\" width=\"87\">100 mL<\/td>\n<td style=\"text-align: center\" width=\"91\">0.48 mM<\/td>\n<td style=\"text-align: center\" width=\"159\">PBS<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"89\">A4239101<\/td>\n<td style=\"text-align: center\" width=\"297\">Gibco\u2122 CTS\u2122 Versene Solution<\/td>\n<td style=\"text-align: center\" width=\"87\">100 mL<\/td>\n<td style=\"text-align: center\" width=\"91\">0.48 mM<\/td>\n<td style=\"text-align: center\" width=\"159\">PBS<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"89\">13150016<\/td>\n<td style=\"text-align: center\" width=\"297\">Gibco\u2122 Cell Dissociation Buffer, enzyme-free, Hanks&#8217; Balanced Salt Solution<\/td>\n<td style=\"text-align: center\" width=\"87\">100 mL<\/td>\n<td style=\"text-align: center\" width=\"91\">\u975e\u516c\u958b<\/td>\n<td style=\"text-align: center\" width=\"159\">HBSS w\/o Ca<sup>2+<\/sup> &amp; Mg<sup>2+<\/sup><br \/>\n\u8a73\u7d30\u306f\u975e\u516c\u958b<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\" width=\"89\">13151014<\/td>\n<td style=\"text-align: center\" width=\"297\">Gibco\u2122 Cell Dissociation Buffer,<br \/>\nenzyme-free, PBS<\/td>\n<td style=\"text-align: center\" width=\"87\">100 mL<\/td>\n<td style=\"text-align: center\" width=\"91\">\u975e\u516c\u958b<\/td>\n<td style=\"text-align: center\" width=\"159\">PBS w\/o Ca<sup>2+<\/sup> &amp; Mg<sup>2+<\/sup><br \/>\n\u8a73\u7d30\u306f\u975e\u516c\u958b<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>Versene solution\u306f0.48 mM\u306eEDTA\u304c\u542b\u307e\u308c\u308bPBS\u3067\u3059\u3002\u4e00\u65b9\u3001HBSS\u30d9\u30fc\u30b9\u3068PBS\u30d9\u30fc\u30b9\u306e\u30e9\u30a4\u30f3\u30ca\u30c3\u30d7\u304c\u3042\u308bCell Dissociation Buffer\u306f\u3001Buffer\u306e\u7d44\u6210\u3068EDTA\u306e\u6fc3\u5ea6\u304c\u7d30\u80de\u89e3\u96e2\u306e\u305f\u3081\u306b\u6700\u9069\u5316\u3055\u308c\u3066\u304a\u308a\u3001\u8a73\u7d30\u306f\u975e\u516c\u958b\u3068\u306a\u3063\u3066\u3044\u307e\u3059\u3002<\/p>\n<h2>\uff08\u88dc\u8db3\uff09\u5206\u6790\u8a3c\u660e\u66f8\uff08Certificate of Analysis\u3001COA\uff09\u306e\u5165\u624b\u65b9\u6cd5<\/h2>\n<p>Collagenase, Type I, powder\uff08\u88fd\u54c1\u756a\u53f717100017\uff09\u306e\u30ed\u30c3\u30c82789756\u306e\u5206\u6790\u8a3c\u660e\u66f8\u3092\u5165\u624b\u3059\u308b\u5834\u5408\u3092\u4f8b\u306b\u5165\u624b\u65b9\u6cd5\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n<p>\u2460\u5f53\u793e\u306eWeb\u30b5\u30a4\u30c8\uff08https:\/\/www.thermofisher.com\/jp\/ja\/home.html\uff09\u306b\u30a2\u30af\u30bb\u30b9<br \/>\n\u2461\u691c\u7d22\u7a93\u306e\u30d7\u30eb\u30c0\u30a6\u30f3\u30e1\u30cb\u30e5\u30fc\u3092\u300c\u5206\u6790\u8a3c\u660e\u66f8\u300d\u306b\u5909\u66f4\u3059\u308b<br \/>\n\u2462\u691c\u7d22\u7a93\u306b\u30ed\u30c3\u30c8\u756a\u53f7\u300c2789756\u300d\u3092\u5165\u529b\u3057\u3066Enter\u30ad\u30fc\u307e\u305f\u306f\u691c\u7d22\u30dc\u30bf\u30f3\u3092\u62bc\u3059<br \/>\n\u2463\u300cLot # 2789756\u300d\u306e\u30ea\u30f3\u30af\u3092\u30af\u30ea\u30c3\u30af\u3059\u308b<br \/>\n\u2464\u5206\u6790\u8a3c\u660e\u66f8\u3092\u78ba\u8a8d\u3057\u3001\u5fc5\u8981\u306b\u5fdc\u3058\u3066PDF\u3067\u4fdd\u5b58\u3059\u308b<br \/>\n\u203b\u5206\u6790\u8a3c\u660e\u66f8\u304c\u898b\u3064\u304b\u3089\u306a\u3044\u5834\u5408\u306f<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/qa-inquiry-form-jp.html?CID=bid_clb_r04_jp_cp0000_pjt0000_bid00000_0so_blg_cell_culture_cell_separation_bid_ts_mbr_24001_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u554f\u5408\u305b\u30d5\u30a9\u30fc\u30e0<\/a>\u304b\u3089\u3054\u8acb\u6c42\u304f\u3060\u3055\u3044\u3002<br \/>\n<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-large wp-image-16249\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2024\/01\/cell-culture-cell-separation-bid-ts-mbr-24001-02-1024x968.png\" alt=\"\" width=\"760\" height=\"718\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2024\/01\/cell-culture-cell-separation-bid-ts-mbr-24001-02-1024x968.png 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2024\/01\/cell-culture-cell-separation-bid-ts-mbr-24001-02-300x284.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2024\/01\/cell-culture-cell-separation-bid-ts-mbr-24001-02-768x726.png 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2024\/01\/cell-culture-cell-separation-bid-ts-mbr-24001-02.png 1063w\" sizes=\"auto, (max-width: 760px) 100vw, 760px\" \/><\/p>\n<h2>\u307e\u3068\u3081<\/h2>\n<ul>\n<li>\u3055\u307e\u3056\u307e\u306a\u7d30\u80de\u89e3\u96e2\u8a66\u85ac\u304c\u3042\u308a\u307e\u3059\u3002\u81d3\u5668\u3084\u7d44\u7e54\u306e\u7a2e\u985e\u3084\u5b9f\u9a13\u76ee\u7684\u306b\u5fdc\u3058\u3066\u4f7f\u3044\u5206\u3051\u3066\u304f\u3060\u3055\u3044\u3002<\/li>\n<li>\u9175\u7d20\u7cfb\u306e\u8a66\u85ac\u306f\u6eb6\u89e3\u65b9\u6cd5\u3084\u4fdd\u7ba1\u6761\u4ef6\u306b\u6ce8\u610f\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/li>\n<li>\u9175\u7d20\u7cfb\u306e\u8a66\u85ac\u306f\u3001\u88fd\u9020\u30ed\u30c3\u30c8\u3054\u3068\u306b\u767a\u884c\u3055\u308c\u308b\u5206\u6790\u8a3c\u660e\u66f8\u306b\u8a18\u8f09\u3055\u308c\u305f\u9175\u7d20\u6d3b\u6027\u306e\u8a66\u9a13\u7d50\u679c\u3092\u78ba\u8a8d\u3057\u3066\u6eb6\u89e3\u3001\u4f7f\u7528\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/li>\n<\/ul>\n<h3>\u3010\u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u30113D\u7d30\u80de\u57f9\u990a\uff06\u89e3\u6790\u30cf\u30f3\u30c9\u30d6\u30c3\u30af<\/h3>\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/download-3d-cell-culture-analysis-handbook-form.html?CID=bid_clb_r04_jp_cp0000_pjt0000_bid00000_0so_blg_cell_culture_cell_separation_bid_ts_mbr_24001_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u306f\u3053\u3061\u3089<\/a><\/p>\n<h3>\u3010\u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3011Gibco\u591a\u80fd\u6027\u5e79\u7d30\u80de\u30cf\u30f3\u30c9\u30d6\u30c3\u30af<\/h3>\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/stem-cell-handbook-dl.html?CID=bid_clb_r04_jp_cp0000_pjt0000_bid00000_0so_blg_cell_culture_cell_separation_bid_ts_mbr_24001_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u306f\u3053\u3061\u3089<\/a><\/p>\n<p>&nbsp;<\/p>\n<p>\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u7528\u306b\u306f\u4f7f\u7528\u3044\u305f\u3060\u3051\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u8fd1\u5e74\u306e\u6025\u6fc0\u306a\u6280\u8853\u9032\u6b69\u306b\u3088\u308b\u6b21\u4e16\u4ee3\u30b7\u30fc\u30b1\u30f3\u31f2\u306e\u30c7\u30fc\u30bf\u53d6\u5f97\u30b3\u30b9\u30c8\u306e\u4f4e\u4e0b\u306b\u3088\u308a\u3001\u30b7\u30f3\u30b0\u30eb\u30bb\u30ebRNA\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\uff08single-cell 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