{"id":165,"date":"2020-03-04T00:00:00","date_gmt":"2020-03-03T15:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic13\/"},"modified":"2026-02-20T13:13:20","modified_gmt":"2026-02-20T04:13:20","slug":"protein-basic13","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic13\/","title":{"rendered":"\u3055\u307e\u3056\u307e\u306a\u6297\u4f53\u7cbe\u88fd\u6cd5\u3092\u307e\u3068\u3081\u3066\u307f\u305f \u305d\u306e2\uff5c\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\uff01\u30bf\u30f3\u30d1\u30af\u8cea\u5b9f\u9a13\u3042\u308c\u3053\u308c \u7b2c13\u56de"},"content":{"rendered":"<h2>\u306f\u3058\u3081\u306b<\/h2>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic12\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa44_Social_LAB\" target=\"_blank\">\u524d\u56de<\/a>\u306f\u7c97\u7cbe\u88fd\u7a0b\u5ea6\u306e\u65b9\u6cd5\uff08Crude\u30ec\u30d9\u30eb\u306e\u7cbe\u88fd\uff09\u3068\u6297\u4f53\u30af\u30e9\u30b9\uff08\u307e\u305f\u306f\u30a2\u30a4\u30bd\u30bf\u30a4\u30d7\uff09\u306e\u30ec\u30d9\u30eb\u3067\u7cbe\u88fd\u3059\u308b\u65b9\u6cd5\uff08\u6297\u4f53\u30af\u30e9\u30b9\u30ec\u30d9\u30eb\u306e\u7cbe\u88fd\uff09\u306b\u3064\u3044\u3066\u7d39\u4ecb\u3057\u307e\u3057\u305f\u3002\u4eca\u56de\u306f\u3001\u76ee\u7684\u6297\u539f\u306b\u7279\u7570\u7684\u306a\u6297\u4f53\u7cbe\u88fd\u3092\u884c\u3046\u65b9\u6cd5\uff08Specific\u306a\u7cbe\u88fd\uff09\u306b\u3064\u3044\u3066\u7d39\u4ecb\u3057\u307e\u3059\u3002\n[ss url=&#8221;https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic12\/&#8221; width=&#8221;180&#8243; class=&#8221;alignleft&#8221; alt=&#8221;\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\uff01\u30bf\u30f3\u30d1\u30af\u8cea\u5b9f\u9a13\u3042\u308c\u3053\u308c \u7b2c12\u56de&#8221; rel=&#8221;nofollow&#8221; ext=0 title=&#8221;\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\uff01\u30bf\u30f3\u30d1\u30af\u8cea\u5b9f\u9a13\u3042\u308c\u3053\u308c \u7b2c12\u56de \u3055\u307e\u3056\u307e\u306a\u6297\u4f53\u7cbe\u88fd\u6cd5\u3092\u307e\u3068\u3081\u3066\u307f\u305f \u305d\u306e1&#8243; caption=&#8221;\u6297\u4f53\u306e\u7cbe\u88fd\u65b9\u6cd5\u306b\u306f\u3001\u5927\u304d\u304f\u5206\u3051\u3066\u3001Crude\u30ec\u30d9\u30eb\u306e\u7cbe\u88fd\u3001\u6297\u4f53\u30af\u30e9\u30b9\u30ec\u30d9\u30eb\u306e\u7cbe\u88fd\u3001Specific\u306a\u7cbe\u88fd\u306e3\u3064\u304c\u3042\u308a\u307e\u3059\u3002\u4eca\u56de\u306f\u305d\u308c\u305e\u308c\u306e\u30ec\u30d9\u30eb\u3054\u3068\u306b\u7cbe\u88fd\u65b9\u6cd5\u3092\u6319\u3052\u3066\u305d\u306e\u6982\u8981\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\uff01&#8221;]\n<h2>Specific\u306a\u7cbe\u88fd<\/h2>\n\u76ee\u7684\u6297\u539f\u306b\u5bfe\u3059\u308b\u6297\u4f53\u4ee5\u5916\u306e\u514d\u75ab\u30b0\u30ed\u30d6\u30ea\u30f3\u3092\u9664\u53bb\u3057\u3066\u3001\u3067\u304d\u308b\u3060\u3051<strong>\u7279\u7570\u7684\u306a\u6297\u4f53\u306e\u307f\u3092\u7cbe\u88fd\u3057\u305f\u3044\u5834\u5408\u306b\u306f\u3001\u6297\u539f\u3092\u56fa\u5b9a\u5316\u3057\u305f\u62c5\u4f53\uff08\u652f\u6301\u4f53\uff09\u3092\u7528\u3044\u308b\u65b9\u6cd5\u304c\u4e00\u822c\u7684\u3067\u3059\u3002<\/strong>\u3053\u306e\u65b9\u6cd5\u3067\u306e\u7cbe\u88fd\u3092\u52b9\u7387\u3088\u304f\u884c\u3046\u305f\u3081\u306b\u306f\u3001\u76ee\u7684\u6297\u539f\u306e\u30a8\u30d4\u30c8\u30fc\u30d7\u3068\u6297\u4f53\u3068\u306e\u7d50\u5408\u304c\u963b\u5bb3\u3055\u308c\u306a\u3044\u3088\u3046\u306b\u6297\u539f\u3092\u62c5\u4f53\u306b\u56fa\u5b9a\u5316\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u7279\u306b\u6297\u539f\u306e\u5206\u5b50\u91cf\u304c\u5c0f\u3055\u3044\u5834\u5408\u306f\u3001\u6297\u539f\u3092\u62c5\u4f53\u306b\u76f4\u63a5\u5316\u5b66\u7684\u306b\u7d50\u5408\u3055\u305b\u308b\u3068\u30a8\u30d4\u30c8\u30fc\u30d7\u304c\u30d6\u30ed\u30c3\u30af\u3055\u308c\u3066\u3057\u307e\u3046\u3053\u3068\u3084\u3001\u7acb\u4f53\u969c\u5bb3\u306b\u3088\u308a\u6297\u4f53\u304c\u30a8\u30d4\u30c8\u30fc\u30d7\u306b\u30a2\u30af\u30bb\u30b9\u3067\u304d\u306a\u304f\u306a\u308b\u3053\u3068\u304c\u3042\u308b\u305f\u3081\u3001\u7279\u5b9a\u306e\u5b98\u80fd\u57fa\uff08\u30a2\u30df\u30ce\u57fa\u3084\u30b9\u30eb\u30d5\u30d2\u30c9\u30ea\u30eb\u57fa\u306a\u3069\uff09\u3068\u53cd\u5fdc\u3059\u308b\u53cd\u5fdc\u57fa\uff08NHS\u30a8\u30b9\u30c6\u30eb\u3084\u30e8\u30fc\u30c9\u30a2\u30bb\u30c1\u30eb\u57fa\u306a\u3069\uff09\u304c\u3001\u3042\u308b\u7a0b\u5ea6\u306e\u30b9\u30da\u30fc\u30b5\u30fc\u30a2\u30fc\u30e0\u3092\u6301\u3063\u305f\u5f62\u3067\u56fa\u5b9a\u5316\u3057\u3066\u3044\u308b\u62c5\u4f53\u3092\u5229\u7528\u3057\u307e\u3059\u3002\u6297\u539f\u3092\u30ad\u30e3\u30ea\u30a2\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u67b6\u6a4b\u3057\u3066\u6297\u4f53\u7523\u751f\u3092\u884c\u3063\u305f\u5834\u5408\u306f\u3001\u901a\u5e38\u3001\u305d\u306e\u67b6\u6a4b\u53cd\u5fdc\u3068\u540c\u3058\u5b98\u80fd\u57fa\u3068\u53cd\u5fdc\u57fa\u3092\u5229\u7528\u3059\u308b\u3053\u3068\u3067\u3001\u30a8\u30d4\u30c8\u30fc\u30d7\u306e\u30d6\u30ed\u30c3\u30af\u3092\u9632\u3050\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\n\n\u6297\u539f\u3068\u6297\u4f53\u3068\u306e\u30a2\u30d5\u30a3\u30cb\u30c6\u30a3\u30fc\u306f\u3001\u751f\u7406\u7684\u6761\u4ef6\u4e0b\u306b\u304a\u3044\u3066\u9ad8\u3044\u305f\u3081\u3001\u901a\u5e38\u3001\u7d50\u5408\u30d0\u30c3\u30d5\u30a1\u30fc\u306b\u306f0.15M NaCl\u3092\u542b\u3080pH 7.2\u7a0b\u5ea6\u306ePBS\u3084TBS\u304c\u4f7f\u7528\u3055\u308c\u307e\u3059\u3002\u307e\u305f\u3053\u306e\u30d0\u30c3\u30d5\u30a1\u30fc\u306b\u66f4\u306b\u5869\u3084\u754c\u9762\u6d3b\u6027\u5264\u3092\u6dfb\u52a0\u3057\u3001\u975e\u7279\u7570\u7684\u306a\u7d50\u5408\u3092\u9664\u53bb\u3059\u308b\u305f\u3081\u306e\u6d17\u6d44\u30d0\u30c3\u30d5\u30a1\u30fc\u3068\u3057\u3066\u5229\u7528\u3055\u308c\u307e\u3059\u3002\u3053\u306e\u9ad8\u3044\u30a2\u30d5\u30a3\u30cb\u30c6\u30a3\u30fc\u7d50\u5408\u3092\u89e3\u96e2\u3055\u305b\u3066\u76ee\u7684\u6297\u4f53\u3092\u7cbe\u88fd\u3059\u308b\u305f\u3081\u306b\u306f\u3001\u751f\u7406\u7684\u6761\u4ef6\u304b\u3089\u5916\u308c\u305f\u6761\u4ef6\u3067\u51e6\u7406\uff08\u6eb6\u51fa\uff09\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u3001\u901a\u5e38\u3001pH \u304c2.5-3.0\u306e\u30b0\u30ea\u30b7\u30f3\u304c\u5229\u7528\u3055\u308c\u307e\u3059\u3002\u4e00\u822c\u7684\u306b\u6297\u4f53\u306fpH\u304c2.5\uff5e11.5\u306e\u7bc4\u56f2\u3067\u306f\u5931\u6d3b\u304c\u8d77\u3053\u308a\u306b\u304f\u3044\u3068\u3055\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u5909\u6027\u3084\u51dd\u96c6\u3092\u907f\u3051\u308b\u305f\u3081\u306b\u6eb6\u51fa\u76f4\u5f8c\u306b\u9069\u5f53\u306a\u30d0\u30c3\u30d5\u30a1\u30fc\u306b\u3088\u308a\u4e2d\u548c\u3057\u3066pH\u3092\u751f\u7406\u7684\u6761\u4ef6\u4e0b\u306b\u8abf\u6574\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u4ee5\u4e0b\u3001\u6297\u539f\u56fa\u5b9a\u5316\u7528\u62c5\u4f53\u306e\u4ee3\u8868\u4f8b\u3092\u7d39\u4ecb\u3057\u307e\u3059\u3002\n<h2>\u6297\u539f\u306e\u30a2\u30df\u30ce\u57fa\uff08-NH2\u57fa\uff09\u3092\u5229\u7528\u3057\u305f\u56fa\u5b9a<\/h2>\n<h3>NHS\u30a8\u30b9\u30c6\u30eb\u6d3b\u6027\u5316\u30a2\u30ac\u30ed\u30fc\u30b9<\/h3>\n<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/26200?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa44_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Pierce\u2122 NHS-Activated Agarose Slurry<\/a>\u306f\u3001N-hydro xysuccinimide (NHS) \u30a8\u30b9\u30c6\u30eb\u57fa\u3092\u672b\u7aef\u306b\u6301\u30646%\u67b6\u6a4b\u30a2\u30ac\u30ed\u30fc\u30b9\u62c5\u4f53\u3067\u3059\u3002<strong>NHS\u30a8\u30b9\u30c6\u30eb\u57fa\u306f\u30bf\u30f3\u30d1\u30af\u8cea\u3084\u305d\u306e\u4ed6\u306e\u30ea\u30ac\u30f3\u30c9\u5206\u5b50\u306b\u5b58\u5728\u3059\u308b\u30a2\u30df\u30ce\u57fa\u3068\u53cd\u5fdc\u3057\u3066\u5b89\u5b9a\u306a\u30a2\u30df\u30c9\u7d50\u5408\u3092\u5f62\u6210\u3057\u307e\u3059\u3002<\/strong>\n\n<div id=\"attachment_519\" style=\"width: 452px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-content\/uploads\/2015\/11\/protein-basic13-fig1.jpg\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-519\" class=\"wp-image-519 size-full\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/protein-basic13-fig1-e1446448175228.jpg\" alt=\"protein-basic13-fig1\" width=\"442\" height=\"122\" \/><\/a><p id=\"caption-attachment-519\" class=\"wp-caption-text\">\u56f31 Pierce\u2122 NHS-Activated Agarose\u3078\u306e\u6297\u4f53\u30ab\u30c3\u30d7\u30ea\u30f3\u30b0\u53cd\u5fdc\u306e\u6982\u7565\u56f3<\/p><\/div>\n<h3>\u30a2\u30eb\u30c7\u30d2\u30c9\u6d3b\u6027\u5316\u30a2\u30ac\u30ed\u30fc\u30b9<\/h3>\n<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/20381?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa44_Social_LAB\" target=\"_blank\" rel=\"noopener\">AminoLink\u2122 Coupling Resin<\/a>\u306f\u3001\u30a2\u30eb\u30c7\u30d2\u30c9\u57fa\u3092\u6301\u30644%\u67b6\u6a4b\u30a2\u30ac\u30ed\u30fc\u30b9\u62c5\u4f53\u3067\u3059\u3002\u30bf\u30f3\u30d1\u30af\u8cea\u3084\u305d\u306e\u4ed6\u306e\u30ea\u30ac\u30f3\u30c9\u5206\u5b50\u306b\u5b58\u5728\u3059\u308b\u30a2\u30df\u30ce\u57fa\u3068\u53cd\u5fdc\u3057\u307e\u3059\u3002<strong>\u30a2\u30eb\u30c7\u30d2\u30c9\u57fa\u3068\u30a2\u30df\u30ce\u57fa\u3068\u306e\u7d50\u5408\u306f\u4e0d\u5b89\u5b9a\u306a\u305f\u3081\u3001\u6e29\u548c\u306a\u9084\u5143\u5264\u3067\u51e6\u7406\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u5b89\u5b9a\u306a\u4e8c\u7d1a\u30a2\u30df\u30f3\u7d50\u5408\u3092\u751f\u6210\u3055\u305b\u307e\u3059\u3002<\/strong>\n\n<div id=\"attachment_520\" style=\"width: 416px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-content\/uploads\/2015\/11\/protein-basic13-fig2.jpg\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-520\" class=\"wp-image-520 size-full\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/protein-basic13-fig2-e1446448459255.jpg\" alt=\"protein-basic13-fig2\" width=\"406\" height=\"416\" \/><\/a><p id=\"caption-attachment-520\" class=\"wp-caption-text\">\u56f32 AminoLink\u2122 Support \u56fa\u5b9a\u5316\u53cd\u5fdc<\/p><\/div>\n<h2>\u6297\u539f\u306e\u30b9\u30eb\u30d5\u30d2\u30c9\u30ea\u30eb\u57fa\uff08-SH\u57fa\uff09\u3092\u5229\u7528\u3057\u305f\u56fa\u5b9a<\/h2>\n<h3>\u30e8\u30fc\u30c9\u30a2\u30bb\u30c1\u30eb\u57fa\u6d3b\u6027\u5316\u30a2\u30ac\u30ed\u30fc\u30b9<\/h3>\nSulfoLink\u2122 Coupling Resin\u306f\u3001\u30e8\u30fc\u30c9\u30a2\u30bb\u30c1\u30eb\u57fa\u3092\u672b\u7aef\u306b\u6301\u30644%\u67b6\u6a4b\u30a2\u30ac\u30ed\u30fc\u30b9\u62c5\u4f53\u3067\u3059\u3002<strong>\u9084\u5143\u30b7\u30b9\u30c6\u30a4\u30f3\u6b8b\u57fa\u3092\u542b\u3080\u30bf\u30f3\u30d1\u30af\u8cea\u3084\u305d\u306e\u4ed6\u306e\u30ea\u30ac\u30f3\u30c9\u5206\u5b50\u3068\u53cd\u5fdc\u3057\u3001\u975e\u53ef\u9006\u7684\u306a\u30c1\u30aa\u30a8\u30fc\u30c6\u30eb\u7d50\u5408\u3092\u5f62\u6210\u3057\u307e\u3059\u3002<\/strong>\n\n<div id=\"attachment_521\" style=\"width: 409px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-content\/uploads\/2015\/11\/protein-basic13-fig3.jpg\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-521\" class=\"wp-image-521 size-full\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/protein-basic13-fig3-e1446448681178.jpg\" alt=\"protein-basic13-fig3\" width=\"399\" height=\"365\" \/><\/a><p id=\"caption-attachment-521\" class=\"wp-caption-text\">\u56f33 Thermo Scientific SulfoLink Support \u56fa\u5b9a\u5316\u53cd\u5fdc\u306e\u6982\u7565\u56f3<\/p><\/div>\n<h2>\u6297\u539f\u306e\u30ab\u30eb\u30dc\u30ad\u30b7\u30eb\u57fa\uff08-COOH\u57fa\uff09\u3092\u5229\u7528\u3057\u305f\u56fa\u5b9a<\/h2>\n<h3>DADPA\u6d3b\u6027\u5316\u30a2\u30ac\u30ed\u30fc\u30b9<\/h3>\n<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/20266B?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa44_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">CarboxyLink\u2122 Coupling Resin<\/a>\u306f\u3001\u30b8\u30a2\u30df\u30ce\u30b8\u30d7\u30ed\u30d4\u30eb\u30a2\u30df\u30f3\uff08DADPA\uff09\u3067\u6d3b\u6027\u304b\u3055\u308c\u305f4%\u67b6\u6a4b\u30a2\u30ac\u30ed\u30fc\u30b9\u62c5\u4f53\u3067\u3059\u3002\u9577\u3044\u30b9\u30da\u30fc\u30b5\u30fc\u30a2\u30fc\u30e0\u306e\u672b\u7aef\u306b\u30a2\u30df\u30ce\u57fa\u3092\u6301\u3061\u30011-Ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC\u3001EDAC\u3068\u3082\u8a00\u3046)\u3068\u5171\u306b\u3001<strong>\u30ab\u30eb\u30dc\u30ad\u30b7\u30eb\u57fa\u3092\u542b\u3080\u5206\u5b50\u3092\u53cd\u5fdc\u3055\u305b\u308b\u3068\u5b89\u5b9a\u306a\u30a2\u30df\u30c9\u7d50\u5408\u3092\u5f62\u6210\u3057\u307e\u3059\u3002<\/strong>\n\n<div id=\"attachment_522\" style=\"width: 410px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-content\/uploads\/2015\/11\/protein-basic13-fig4.jpg\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-522\" class=\"wp-image-522 size-full\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/protein-basic13-fig4-e1446448807806.jpg\" alt=\"protein-basic13-fig4\" width=\"400\" height=\"322\" \/><\/a><p id=\"caption-attachment-522\" class=\"wp-caption-text\">\u56f34 CarboxyLink Support \u3078\u306e\u6297\u4f53\u56fa\u5b9a\u5316\u53cd\u5fdc<\/p><\/div>\n<h3>\u30d2\u30c9\u30e9\u30b8\u30c9\u6d3b\u6027\u5316\u30a2\u30ac\u30ed\u30fc\u30b9<\/h3>\n<a href=\"https:\/\/www.lifetechnologies.com\/order\/catalog\/product\/20266B?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa44_Social_LAB\" target=\"_blank\" rel=\"noopener\">CarboxyLink\u2122 Coupling Resin<\/a>\u306f\u3001\u30d2\u30c9\u30e9\u30b8\u30c9\u3067\u6d3b\u6027\u5316\u3055\u308c\u305f6%\u67b6\u6a4b\u30a2\u30ac\u30ed\u30fc\u30b9\u62c5\u4f53\u3067\u3059\u3002\u7cd6\u30bf\u30f3\u30d1\u30af\u8cea\u3084\u6297\u4f53\u306e\u7cd6\u9396\u306b\u5b58\u5728\u3059\u308b\u30b7\u30b9-\u30b8\u30aa\u30fc\u30eb\u57fa\uff08cis-diol\u57fa\uff09\u3092\u9178\u5316\u3057\u3066\u751f\u3058\u308b\u30a2\u30eb\u30c7\u30d2\u30c9\u57fa\uff08-CHO\u57fa\uff09\u3068\u30d2\u30c9\u30e9\u30b8\u30c9\u57fa\u3068\u306e\u53cd\u5fdc\u306b\u3088\u308a\u3001<strong>\u5b89\u5b9a\u306a\u30d2\u30c9\u30e9\u30be\u30f3\u7d50\u5408\u3092\u5f62\u6210\u3057\u307e\u3059\u3002<\/strong>\n\n<div id=\"attachment_524\" style=\"width: 346px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-content\/uploads\/2015\/11\/protein-basic13-fig5.jpg\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-524\" class=\"wp-image-524 size-full\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/protein-basic13-fig5-e1446448994897.jpg\" alt=\"protein-basic13-fig5\" width=\"336\" height=\"371\" \/><\/a><p id=\"caption-attachment-524\" class=\"wp-caption-text\">\u56f35 CarboLink Support\u3078\u306e\u6297\u4f53\u56fa\u5b9a\u5316\u53cd\u5fdc<\/p><\/div>\n<h2>\u6700\u5f8c\u306b<\/h2>\n\u3044\u304b\u304c\u3067\u3057\u3087\u3046\u304b\uff1f\n\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic12\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa44_Social_LAB\" target=\"_blank\">\u524d\u56de<\/a>\u3068\u4eca\u56de\u3067\u6297\u4f53\u7cbe\u88fd\u306e\u3055\u307e\u3056\u307e\u306a\u65b9\u6cd5\u3092\u7d39\u4ecb\u3057\u307e\u3057\u305f\u3002\u5b9f\u969b\u306b\u306f\u3054\u7d39\u4ecb\u3057\u305f\u65b9\u6cd5\u4ee5\u5916\u306b\u3082\u3001\u4e00\u822c\u7684\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u7cbe\u88fd\u306b\u4f7f\u7528\u3055\u308c\u308b\u30b2\u30eb\u308d\u904e\u3084\u30a4\u30aa\u30f3\u4ea4\u63db\u306a\u3069\u3082\u4f7f\u7528\u3055\u308c\u308b\u3053\u3068\u304c\u3042\u308a\u3001\u3055\u307e\u3056\u307e\u306a\u624b\u6cd5\u3092\u7d44\u5408\u305b\u3066\u6bb5\u968e\u7684\u306b\u3001\u3088\u308a\u7d14\u5ea6\u306e\u9ad8\u3044\u6297\u4f53\u7cbe\u88fd\u304c\u884c\u308f\u308c\u307e\u3059\u3002\u6b21\u56de\u306f\u3001\u524d\u56de\u3067\u7d39\u4ecb\u3057\u305f\u5404\u6297\u4f53\u7d50\u5408\u30bf\u30f3\u30d1\u30af\u8cea\uff08Protein A, Protein G, Protein A\/G, Protein L\uff09\u306b\u3064\u3044\u3066\u3054\u7d39\u4ecb\u3057\u307e\u3059\uff01\n\n<h2 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class=\"btn btn-red\" href=\"http:\/\/thermofisher.com\/jp\/ja\/home\/global\/forms\/crosslinker-handbook.html?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa44_Social_LAB\" target=\"_blank\" rel=\"noreferrer noopener\">\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/p>\n\n\n<h2>\u3010\u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3011\u30bf\u30f3\u30d1\u30af\u8cea\u89e3\u6790\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u30cf\u30f3\u30c9\u30d6\u30c3\u30af<\/h2>\n<p>\u52b9\u7387\u7684\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u62bd\u51fa\u304b\u3089\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u89e3\u6790\u30c4\u30fc\u30eb\u307e\u3067\u3001\u5305\u62ec\u7684\u306b\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u3092\u7d39\u4ecb\u3057\u3066\u304a\u308a\u307e\u3059\u3002PDF\u30d5\u30a1\u30a4\u30eb\u306e\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3092\u3054\u5e0c\u671b\u306e\u65b9\u306f\u3001\u4e0b\u8a18\u30dc\u30bf\u30f3\u3088\u308a\u304a\u7533\u8fbc\u307f\u304f\u3060\u3055\u3044\u3002<\/p>\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/catalogs-protein-form.html?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa44_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/p><h2>\u5171\u514d\u75ab\u6c88\u964d\u6cd5\u95a2\u9023\u3001\u305d\u306e\u4ed6\u306e\u8a18\u4e8b\u306f\u3053\u3061\u3089<\/h2>\n<table style=\"border-color: lightgray;border-collapse: collapse;padding: 20px\" border=\"1\">\n<tbody>\n<tr>\n<td style=\"border-color: lightgray;padding: 20px\">\n<ul>\n \t<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic8\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa44_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u7b2c8\u56de\u3011in vitro \u30bf\u30f3\u30d1\u30af\u8cea\u76f8\u4e92\u4f5c\u7528\u3092\u89e3\u6790\u3059\u308b\u65b9\u6cd5\u307e\u3068\u3081\n<\/a><\/li>\n \t<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic9\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa44_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u7b2c9\u56de\u3011\u5171\u514d\u75ab\u6c88\u964d\u6cd5\u306e\u300c\u554f\u984c\u70b9\u300d\u3068\u300c\u6539\u5584\u7b56\u300d\u307e\u3068\u3081\n<\/a><\/li>\n \t<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic12\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa44_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u7b2c12\u56de\u3011\u3055\u307e\u3056\u307e\u306a\u6297\u4f53\u7cbe\u88fd\u6cd5\u3092\u307e\u3068\u3081\u3066\u307f\u305f \u305d\u306e1\n<\/a><\/li>\n \t<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic14\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa44_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u7b2c14\u56de\u3011\u307f\u3093\u306a\u304c\u77e5\u308a\u305f\u3044\u30d7\u30ed\u30c6\u30a4\u30f3A\/G\/L\u306e\u6027\u8cea\n<\/a><\/li>\n \t<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic21\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa44_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u7b2c21\u56de\u3011\u5171\u514d\u75ab\u6c88\u964d\u5b9f\u9a13\u306e\u57fa\u672c\u7684\u306a\u5b9f\u9a13\u624b\u9806\n<\/a><\/li>\n \t<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic22\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa44_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u7b2c22\u56de\u3011\u5171\u514d\u75ab\u6c88\u964d\u5b9f\u9a13\u306b\u304a\u3051\u308b\u6297\u4f53\u306e\u56fa\u5b9a\u5316\n<\/a><\/li>\n \t<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic23\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa44_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u7b2c23\u56de\u3011\u5171\u514d\u75ab\u6c88\u964d\u5b9f\u9a13\u306b\u304a\u3051\u308b\u30d0\u30c3\u30d5\u30a1\u30fc\u306b\u3064\u3044\u3066 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