{"id":17222,"date":"2024-05-28T13:39:59","date_gmt":"2024-05-28T04:39:59","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/?p=17222"},"modified":"2024-09-18T15:58:17","modified_gmt":"2024-09-18T06:58:17","slug":"sanger-seq-mutational-analysis-gsd-ts-ce-24029","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/sanger-seq-mutational-analysis-gsd-ts-ce-24029\/","title":{"rendered":"\u5168\u8eab\u6027\u30a8\u30ea\u30c6\u30de\u30c8\u30fc\u30c7\u30b9\u60a3\u8005\u306e\u7a81\u7136\u5909\u7570\u306e\u767a\u898b\u3068\u3001\u65b0\u305f\u306a\u6cbb\u7642\u6cd5\u306e\u958b\u62d3"},"content":{"rendered":"

\u30b9\u30da\u30a4\u30f3\u306e7\u6b73\u5973\u5150\u304c\u767a\u75c7\u3057\u305f\u3001\u7570\u5e38\u306a\u5168\u8eab\u6027\u30a8\u30ea\u30c6\u30de\u30c8\u30fc\u30c7\u30b9\u3092\u8abf\u67fb\u3059\u308b\u305f\u3081\u306b\u3001\u4e16\u754c\u5404\u5730\u304b\u3089\u7814\u7a76\u8005\u30c1\u30fc\u30e0\u304c\u96c6\u7d50\u3057\u307e\u3057\u305f\u3002\u5168\u30b2\u30ce\u30e0\u30b7\u30fc\u30b1\u30f3\u30b9\u3067\u3001\u75c5\u6c17\u306e\u63a8\u5b9a\u539f\u56e0\u3068\u3057\u3066\u4e00\u3064\u306e\u907a\u4f1d\u5b50\u7570\u5e38\uff08TLR7\u907a\u4f1d\u5b50\u306e\u70b9\u5909\u7570\uff09\u304c\u5224\u660e\u3057\u307e\u3057\u305f\u3002\u3053\u306e\u5909\u7570\u3092\u30de\u30a6\u30b9\u306b\u5c0e\u5165\u3057\u305f\u3068\u3053\u308d\u3001\u5909\u7570\u30de\u30a6\u30b9\u306b\u5168\u8eab\u6027\u30a8\u30ea\u30c6\u30de\u30c8\u30fc\u30c7\u30b9\u306e\u3088\u3046\u306a\u75c7\u72b6\u304c\u73fe\u308c\u3001\u5f7c\u3089\u304c\u767a\u898b\u3057\u305f\u70b9\u5909\u7570\u304c\u5b9f\u969b\u306e\u539f\u56e0\u3067\u3042\u308b\u3053\u3068\u304c\u78ba\u8a8d\u3055\u308c\u307e\u3057\u305f1<\/sup>\u3002\u3053\u306e\u75be\u60a3\u306b\u82e6\u3057\u3080\u5c0f\u3055\u306a\u30b9\u30da\u30a4\u30f3\u306e\u5973\u5150\u306e\u3088\u3046\u306a\u60a3\u8005\u306b\u3068\u3063\u3066\u3001\u3053\u308c\u306f\u3069\u306e\u3088\u3046\u306a\u610f\u5473\u3092\u6301\u3064\u306e\u3067\u3057\u3087\u3046\u304b\uff1f\u307e\u305f\u3001\u65b0\u305f\u306a\u6a19\u7684\u6cbb\u7642\u6cd5\u306f\u767a\u898b\u3055\u308c\u308b\u306e\u3067\u3057\u3087\u3046\u304b\uff1f<\/p>\n

\u5168\u8eab\u6027\u30a8\u30ea\u30c6\u30de\u30c8\u30fc\u30c7\u30b9\u75ab\u5b66\u3001\u75c7\u72b6\u3001\u73fe\u5728\u306e\u6cbb\u7642\u6cd5<\/h2>\n

\u5168\u8eab\u30a8\u30ea\u30c6\u30de\u30c8\u30fc\u30c7\u30b9\uff08Systemic lupus erythematosus\uff1aSLE\uff09\u306f\u3001\u58ca\u6ec5\u7684\u306a\u81ea\u5df1\u514d\u75ab\u75be\u60a3\u3067\u3042\u308a\u3001\u5168\u4e16\u754c\u3067\u7d04341\u4e07\u4eba\u304c\u7f79\u60a3\u3057\u3066\u3044\u307e\u3059\u3002\u5973\u6027\u306e\u7f79\u60a3\u7387\u304c\u9ad8\u304f\u3001\u7537\u6027\u304c36\u4e07\u4eba\u3067\u3042\u308b\u306e\u306b\u5bfe\u3057\u3066\u3001\u5973\u6027\u306f\u7d04304\u4e07\u4eba\u304c\u7f79\u60a3\u3057\u3066\u3044\u307e\u30592<\/sup>\u3002\u907a\u4f1d\u7684\u8981\u56e0\u3068\u3001\u9069\u5fdc\u514d\u75ab\u7cfb\u304a\u3088\u3073\u81ea\u7136\u514d\u75ab\u7cfb\u306e\u8abf\u7bc0\u969c\u5bb3\u3068\u306e\u9593\u3067\u8d77\u3053\u308b\u8907\u96d1\u306a\u76f8\u4e92\u4f5c\u7528\u304c\u3001SLE\u60a3\u8005\u306b\u304a\u3044\u3066\u306f\u708e\u75c7\u3068\u7d44\u7e54\u969c\u5bb3\u3092\u5f15\u304d\u8d77\u3053\u3057\u307e\u30593<\/sup>\u3002\u75c7\u72b6\u306b\u306f\u500b\u4eba\u5dee\u304c\u3042\u308a\u3001\u300c\u8776\u5f62\u7d05\u6591\u300d\u306b\u4ee3\u8868\u3055\u308c\u308b\u76ae\u819a\u306e\u767a\u75b9\u3001\u7b4b\u8089\u75db\u3001\u95a2\u7bc0\u75db\u3001\u8ca7\u8840\u3001\u75b2\u52b4\u3001\u8131\u6bdb\u3001\u814e\u81d3\u969c\u5bb3\u306a\u3069\u304c\u3042\u308a\u307e\u30594<\/sup>\u3002<\/p>\n

\u6cbb\u7642\u53cd\u5fdc\u6027\u306f\u3055\u307e\u3056\u307e\u3067\u3001\u4e88\u6e2c\u4e0d\u53ef\u80fd\u306a\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u3057\u304b\u3057\u306a\u304c\u3089\u3001\u65e9\u671f\u6cbb\u7642\u3068\u3001\u75be\u75c5\u7ba1\u7406\u306e\u304a\u304b\u3052\u3067\u751f\u5b58\u671f\u9593\u306f\u904e\u53bb50\u5e74\u9593\u3067\u304b\u306a\u308a\u6539\u5584\u3055\u308c\u307e\u3057\u305f3<\/sup>\u3002\u73fe\u5728\u306e\u6cbb\u7642\u6cd5\u306b\u306f\u3001\u30d2\u30c9\u30ed\u30ad\u30b7\u30af\u30ed\u30ed\u30ad\u30f3\u3092\u4ee3\u8868\u3068\u3059\u308b\u6297\u30de\u30e9\u30ea\u30a2\u85ac\u3001\u6301\u7d9a\u7684\u306a\u30b9\u30c6\u30ed\u30a4\u30c9\u85ac\u3001\u9ad8\u7528\u91cf\u6295\u4e0e\u306e\u5316\u5b66\u7642\u6cd5\u3001\u305d\u3057\u3066\u6700\u8fd1\u3067\u306f\u3001\u6a19\u7684\u6307\u5411\u578b\u751f\u7269\u5b66\u7684\u88fd\u5264\u306b\u3088\u308b\u6cbb\u7642\u304c\u3042\u308a\u307e\u30593<\/sup>\u3002<\/p>\n

\u3053\u306e\u3088\u3046\u306a\u9032\u6b69\u306b\u3082\u304b\u304b\u308f\u3089\u305a\u3001SLE\u306e\u6839\u5e95\u306b\u3042\u308b\u591a\u304f\u306e\u30e1\u30ab\u30cb\u30ba\u30e0\u306f\u89e3\u660e\u3055\u308c\u3066\u304a\u3089\u305a\u3001\u3088\u308a\u52b9\u679c\u7684\u306a\u6cbb\u7642\u6cd5\u306e\u958b\u767a\u3084\u3001\u6cbb\u7642\u306e\u53ef\u80fd\u6027\u307e\u3067\u3082\u304c\u963b\u307e\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n

SLE\u306b\u304a\u3051\u308b\u3001Toll\u69d8\u53d7\u5bb9\u4f53\uff08Toll-like receptors\uff1aTLR\uff09<\/h2>\n

Toll\u69d8\u53d7\u5bb9\u4f53\uff08Toll-like receptors\uff1aTLR\uff09\u306fSLE\u306e\u767a\u75c7\u306b\u6975\u3081\u3066\u91cd\u8981\u306a\u5f79\u5272\u3092\u679c\u305f\u3057\u3066\u3044\u307e\u3059\u3002\u75c5\u539f\u4f53\u306e\u30d1\u30bf\u30fc\u30f3\u3092\u8a8d\u8b58\u3059\u308b\u3053\u3068\u3067\u77e5\u3089\u308c\u308bTLR\u306f\u3001\u81ea\u7136\u514d\u75ab\u53cd\u5fdc\u3068\u9069\u5fdc\u514d\u75ab\u53cd\u5fdc\u306e\u53cc\u65b9\u306b\u304a\u3044\u3066\u91cd\u8981\u306a\u69cb\u6210\u8981\u7d20\u3067\u3059\u3002\u4e2d\u3067\u3082\u3001B\u7d30\u80de\u306b\u304a\u3051\u308bTLR7\u3068TLR9\u306e\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u7570\u5e38\u304cSLE\u306e\u767a\u75c7\u306b\u95a2\u4e0e\u3057\u3066\u3044\u307e\u30595<\/sup>\u3002\u30b2\u30ce\u30e0\u30ef\u30a4\u30c9\u95a2\u9023\u89e3\u6790\u3067\u306f\u3001\u3044\u304f\u3064\u304b\u306eTLR7\u591a\u578b\u304cSLE\u3068\u95a2\u9023\u3057\u3066\u3044\u307e\u30596<\/sup>\u3002\u30de\u30a6\u30b9\u30e2\u30c7\u30eb\u3067\u306f\u3001TLR7\u306e\u904e\u5270\u767a\u73fe\u306f\u91cd\u75c7\u306eSLE\u3092\u5f15\u304d\u8d77\u3053\u3057\u307e\u3059\u304c\u3001TLR7\u306e\u6b20\u5931\u306f\u75be\u60a3\u3092\u4e88\u9632\u307e\u305f\u306f\u8efd\u6e1b\u3059\u308b\u3053\u3068\u304c\u5206\u304b\u308a\u307e\u3057\u305f1,7,8<\/sup>\u3002<\/p>\n

\u91cd\u75c7SLE\u3092\u767a\u75c7\u3057\u305f\u3001\u30b9\u30da\u30a4\u30f3\u306e7\u6b73\u5973\u5150\u306e\u30b1\u30fc\u30b9<\/h2>\n

2022\u5e74\u3001Brown\u6c0f\u3089\u306f\u3001\u91cd\u75c7\u60a3\u8005\u3067\u3042\u308b7\u6b73\u306e\u30b9\u30da\u30a4\u30f3\u4eba\u5973\u5150\u306b\u304a\u3044\u3066\u3001SLE\u306e\u6839\u672c\u7684\u306a\u539f\u56e0\u3068\u3057\u3066TLR7\u907a\u4f1d\u5b50\u306e\u6a5f\u80fd\u7372\u5f97\u6027\u70b9\u5909\u7570\u3092\u540c\u5b9a\u3057\u307e\u3057\u305f\u3002\u60a3\u8005\u306e\u5168\u30b2\u30ce\u30e0\u30b7\u30fc\u30b1\u30f3\u30b9\u6c7a\u5b9a\u3068\u30d0\u30a4\u30aa\u30a4\u30f3\u30d5\u30a9\u30de\u30c6\u30a3\u30af\u30b9\u89e3\u6790\u306b\u3088\u308a\u3001de novo\u3067\u3042\u308bTLR7 p.Tyr264His\uff08Y264H\uff09\u306e\u30df\u30b9\u30bb\u30f3\u30b9\u5909\u7570\u304c\u767a\u898b\u3055\u308c\u307e\u3057\u305f\u3002
\n\u56f31 A-B\u306f\u3001Applied Biosystems\u2122 BigDye\u2122 Terminator Cycle Sequencing Kit v3.1\u3092\u7528\u3044\u305f\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u6cd5\u3067\u30013730 DNA \u30a2\u30ca\u30e9\u30a4\u30b6\u30b7\u30b9\u30c6\u30e0\u306b\u3088\u308a\u540c\u5b9a\u3055\u308c\u305f\u60a3\u8005\u5bb6\u65cf\u306eTLR7\u30d0\u30ea\u30a2\u30f3\u30c8\u3092\u793a\u3057\u3066\u3044\u307e\u30591<\/sup>\u3002<\/p>\n

\u540c\u5b9a\u3055\u308c\u305f\u907a\u4f1d\u5b50\u5909\u7570\uff08TLR7Y264H<\/sup>\uff09\u304cSLE\u3092\u5f15\u304d\u8d77\u3053\u3059\u304b\u3069\u3046\u304b\u3092\u8abf\u3079\u308b\u305f\u3081\u3001\u7814\u7a76\u8005\u3089\u306fCRISPR-Cas9\u30b2\u30ce\u30e0\u7de8\u96c6 \u306b\u3088\u3063\u3066\u30de\u30a6\u30b9\u306b\u5909\u7570\u3092\u5c0e\u5165\u3057\u307e\u3057\u305f\u3002
\n\u5909\u7570\u30de\u30a6\u30b9\u306f\u81ea\u5df1\u514d\u75ab\u75c7\u72b6\u3092\u767a\u75c7\u3057\u3001\u672b\u7aef\u81d3\u5668\u306b\u640d\u50b7\u3092\u53d7\u3051\u307e\u3057\u305f\u3002\u3053\u308c\u304c\u3001TLR7\u907a\u4f1d\u5b50\u5909\u7570\u304c\u30d2\u30c8\u306eSLE\u306e\u539f\u56e0\u3068\u3057\u3066\u540c\u5b9a\u3055\u308c\u305f\u6700\u521d\u306e\u4f8b\u3067\u30591<\/sup>\u3002<\/p>\n

\"\u56f31\uff1a

\u56f31\uff1a 7\u6b73\u3067\u91cd\u75c7SLE\u3068\u8a3a\u65ad\u3055\u308c\u305f\u30b9\u30da\u30a4\u30f3\u306e\u5973\u5150\u5bb6\u65cf\u306b\u304a\u3051\u308bTLR7\u5909\u7570\u4f53\uff08A\uff09
Big Dye Terminator Cycle Sequencing Kit v3.1\u3067\u53cd\u5fdc\u30013730 DNA \u30a2\u30ca\u30e9\u30a4\u30b6\u30b7\u30b9\u30c6\u30e0\u3092\u4f7f\u7528\u3057\u3001\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u306b\u3088\u308a\u540c\u5b9a\u3055\u308c\u305f\u5909\u7570\u7b87\u6240\uff08B\uff09
\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u306e\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\uff08C\uff09<\/p><\/div>\n

\u767a\u898b\u304b\u3089\u81e8\u5e8a\u3078 – \u65b0\u305f\u306a\u6cbb\u7642\u6cd5\u3068\u3057\u3066\u306eTLR\u963b\u5bb3<\/h2>\n

2023\u5e74Hawtin\u6c0f\u3089\u306f\u3001\u7d4c\u53e3\u6295\u4e0e\u53ef\u80fd\u306aTLR7\/8\u62ee\u6297\u85ac\u3067\u3042\u308b\u3001MHV370\u306eSLE\u306b\u5bfe\u3059\u308b\u975e\u81e8\u5e8a\u8a66\u9a13\u3092\u767a\u8868\u3057\u307e\u3057\u305f\u3002\u5f7c\u3089\u306f\u3001\u30d2\u30c8\u304a\u3088\u3073\u30de\u30a6\u30b9\u306e\u7d30\u80de\u306b\u304a\u3044\u3066\u3001MHV370\u304cTLR7\/8\u4f9d\u5b58\u6027\u306e\u30b5\u30a4\u30c8\u30ab\u30a4\u30f3\u3001\u7279\u306b\u81ea\u5df1\u514d\u75ab\u75be\u60a3\u306e\u539f\u56e0\u3068\u3057\u3066\u77e5\u3089\u308c\u308b\u3001\u30a4\u30f3\u30bf\u30fc\u30d5\u30a7\u30ed\u30f3\u03b1\u306e\u7523\u751f\u3092\u963b\u5bb3\u3059\u308b\u3053\u3068\u3092\u793a\u3057\u307e\u3057\u305f9<\/sup>\u3002<\/p>\n

\u4f8b\u3048\u3070\u3001\u7814\u7a76\u30c1\u30fc\u30e0\u306fMHV370\u304cin vivo\u30de\u30a6\u30b9\u30e2\u30c7\u30eb\u306b\u304a\u3044\u3066\u3001\u6162\u6027\u7684\u306aTLR7\u4f9d\u5b58\u6027\u514d\u75ab\u6d3b\u6027\u3092\u6291\u5236\u3059\u308b\u3053\u3068\u3092\u793a\u3057\u307e\u3057\u305f9<\/sup>\u3002\u30de\u30a6\u30b93\u7fa4\uff3b\uff081\uff09\u672a\u51e6\u7f6e\u3001\uff082\uff0914\u65e5\u9593R848\u51e6\u7f6e\uff08TLR\u306e\u4f5c\u52d5\u85ac\uff09\u3001\uff083\uff0914\u65e5\u9593R848\u51e6\u7f6e\uff0b7\u65e5\u76ee\u4ee5\u964dMHV370\u51e6\u7f6e\uff3d\u304b\u3089\u5f97\u305f\u8840\u6db2\u3092\u7528\u3044\u305f\u30a2\u30f3\u30d7\u30ea\u30b3\u30f3\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\uff08Ion AmpliSeq\u2122 Transcriptome Mouse Gene Expression Kit\u306b\u3088\u308b\u30c8\u30e9\u30f3\u30b9\u30af\u30ea\u30d7\u30c8\u30fc\u30e0\u30d7\u30ed\u30d5\u30a1\u30a4\u30ea\u30f3\u30b0\u3067\u306f\u3001MHV370\u304c\u30de\u30a6\u30b9\u306e\u6162\u6027\u7684\u306aTLR7\u4f9d\u5b58\u6027\u514d\u75ab\u6d3b\u6027\u3092\u6291\u5236\u3059\u308b\u3053\u3068\u304c\u793a\u3055\u308c\u307e\u3057\u305f\u3002R848\u306e\u307f\u306e\u6295\u4e0e\u7fa4\u3067\u306f\u3001\u3044\u304f\u3064\u304b\u306e\u708e\u75c7\u7d4c\u8def\u304c\u6d3b\u6027\u5316\u3055\u308c\u3066\u3044\u308b\u3053\u3068\u304c\u793a\u3055\u308c\u307e\u3057\u305f\u3002R848\u51e6\u7f6e\u306b\u52a0\u30487\u65e5\u76ee\u4ee5\u964dMHV370\u3067\u51e6\u7f6e\u3057\u305f\u7fa4\u3067\u306f\u3001\u6d3b\u6027\u5316\u3055\u308c\u305f\u708e\u75c7\u7d4c\u8def\u304c\u4e0b\u65b9\u5236\u5fa1\u3055\u308c\u3001MHV370\u304c\u6162\u6027\u7684\u306aTLR7\u306e\u6d3b\u6027\u5316\u72b6\u614b\u306b\u304a\u3044\u3066\u3001\u708e\u75c7\u53cd\u5fdc\u3092\u4fc3\u9032\u3055\u305b\u308b\u30d1\u30e9\u30e1\u30fc\u30bf\u30fc\u3092\u6b63\u5e38\u5316\u3059\u308b\u3053\u3068\u304c\u793a\u3055\u308c\u307e\u3057\u305f\u3002(\u30c7\u30fc\u30bf\u306f\u3053\u3061\u3089\u3092\u3054\u89a7\u304f\u3060\u3055\u3044: https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC10213863\/figure\/fig3\/<\/a>)<\/p>\n

\u3055\u3089\u306b\u7814\u7a76\u30c1\u30fc\u30e0\u306f\u3001MHV370\u304cSLE\u30e2\u30c7\u30eb\u30de\u30a6\u30b9\u306b\u304a\u3044\u3066\u3001\u75be\u60a3\u306e\u767a\u75c7\u3092\u4e88\u9632\u3057\u3001\u505c\u6b62\u3055\u305b\u308b\u3053\u3068\u3092\u793a\u3057\u307e\u3057\u305f\u3002\u3053\u306e\u975e\u81e8\u5e8a\u8a66\u9a13\u306e\u7d50\u679c\u306f\u3001TLR7\/8\u963b\u5bb3\u304cSLE\u306e\u6709\u671b\u306a\u6cbb\u7642\u6cd5\u3067\u3042\u308b\u3053\u3068\u3092\u7acb\u8a3c\u3057\u307e\u3057\u305f9<\/sup>\u3002\u73fe\u5728\u3001\u4ed6\u306e2\u3064\u306e\u81ea\u5df1\u514d\u75ab\u75be\u60a3\u306b\u304a\u3051\u308bMHV370\u306e\u5b89\u5168\u6027\u3001\u5fcd\u5bb9\u6027\u3001\u6709\u52b9\u6027\u3092\u8a55\u4fa1\u3059\u308b\u7b2c\u2161\u76f8\u8a66\u9a13\u304c\u9032\u884c\u4e2d\u3067\u305910<\/sup>\u3002<\/p>\n

Applied Biosystems\u2122 \u30d6\u30e9\u30f3\u30c9\u306e\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u306b\u3088\u3063\u3066\u3001\u5e0c\u5c11\u75be\u60a3\u3084\u907a\u4f1d\u6027\u75be\u60a3\u306e\u30c8\u30e9\u30f3\u30b9\u30ec\u30fc\u30b7\u30e7\u30ca\u30eb\u30ea\u30b5\u30fc\u30c1\u304c\u3069\u306e\u3088\u3046\u306b\u5b9f\u73fe\u3055\u308c\u3066\u3044\u308b\u304b\u306b\u3064\u3044\u3066\u306f\u3001\u3053\u3061\u3089\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002
\nthermofisher.com\/abraredisease<\/a>.<\/p>\n

\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u306e\u57fa\u790e\u3000\u30cf\u30f3\u30c9\u30d6\u30c3\u30af<\/h3>\n

\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u304b\u3089\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u307e\u3067\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u306e\u57fa\u672c\u539f\u5247\u3092\u5b66\u3079\u307e\u3059\u3002<\/p>\n

\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/p>\n

References:
\n1. Brown GJ, Ca\u00f1ete PF, Wang H, Medhavy A, Bones J, Roco JA, He Y, Qin Y, Cappello J, Ellyard JI, Bassett K, Shen Q, Burgio G, Zhang Y, Turnbull C, Meng X, Wu P, Cho E, Miosge LA, Andrews TD, Field MA, Tvorogov D, Lopez AF, Babon JJ, L\u00f3pez CA, G\u00f3nzalez-Murillo \u00c1, Garulo DC, Pascual V, Levy T, Mallack EJ, Calame DG, Lotze T, Lupski JR, Ding H, Ullah TR, Walters GD, Koina ME, Cook MC, Shen N, de Lucas Collantes C, Corry B, Gantier MP, Athanasopoulos V, Vinuesa CG. TLR7 gain-of-function genetic variation causes human lupus. Nature. 2022 May;605(7909):349-356. doi: 10.1038\/s41586-022-04642-z. Epub 2022 Apr 27. PMID: 35477763; PMCID: PMC9095492.
\n2. Tian J, Zhang D, Yao X, Huang Y, Lu Q. Global epidemiology of systemic lupus erythematosus: a comprehensive systematic analysis and modelling study. Ann Rheum Dis. 2023 Mar;82(3):351-356. doi: 10.1136\/ard-2022-223035. Epub 2022 Oct 14. PMID: 36241363; PMCID: PMC9933169.
\n3. Lazar S, Kahlenberg JM. Systemic Lupus Erythematosus: New Diagnostic and Therapeutic Approaches. Annu Rev Med. 2023 Jan 27;74:339-352. doi: 10.1146\/annurev-med-043021-032611. Epub 2022 Jul 8. PMID: 35804480.
\n4. Lupus symptoms:https:\/\/www.cdc.gov\/lupus\/basics\/symptoms.htm<\/a>
\n5. Wen L, Zhang B, Wu X, Liu R, Fan H, Han L, Zhang Z, Ma X, Chu CQ, Shi X. Toll-like receptors 7 and 9 regulate the proliferation and differentiation of B cells in systemic lupus erythematosus. Front Immunol. 2023 Feb 15;14:1093208. doi: 10.3389\/fimmu.2023.1093208. PMID: 36875095; PMCID: PMC9975558.
\n6. Celhar T, Fairhurst AM. Toll-like receptors in systemic lupus erythematosus: potential for personalized treatment. Front Pharmacol. 2014 Dec 8;5:265. doi: 10.3389\/fphar.2014.00265. PMID: 25538618; PMCID: PMC4258990.
\n7. Pisitkun P, Deane JA, Difilippantonio MJ, Tarasenko T, Satterthwaite AB, Bolland S. Autoreactive B cell responses to RNA-related antigens due to TLR7 gene duplication. Science. 2006 Jun 16;312(5780):1669-72. doi: 10.1126\/science.1124978. Epub 2006 May 18. PMID: 16709748.
\n8. Berland R, Fernandez L, Kari E, Han JH, Lomakin I, Akira S, Wortis HH, Kearney JF, Ucci AA, Imanishi-Kari T. Toll-like receptor 7-dependent loss of B cell tolerance in pathogenic autoantibody knockin mice. Immunity. 2006 Sep;25(3):429-40. doi: 10.1016\/j.immuni.2006.07.014. PMID: 16973388.
\n9. Hawtin S, Andr\u00e9 C, Collignon-Zipfel G, Appenzeller S, Bannert B, Baumgartner L, Beck D, Betschart C, Boulay T, Brunner HI, Ceci M, Deane J, Feifel R, Ferrero E, Kyburz D, Lafossas F, Loetscher P, Merz-Stoeckle C, Michellys P, Nuesslein-Hildesheim B, Raulf F, Rush JS, Ruzzante G, Stein T, Zaharevitz S, Wieczorek G, Siegel R, Gergely P, Shisha T, Junt T. Preclinical characterization of the Toll-like receptor 7\/8 antagonist MHV370 for lupus therapy. Cell Rep Med. 2023 May 16;4(5):101036. doi: 10.1016\/j.xcrm.2023.101036. PMID: 37196635; PMCID: PMC10213863.
\n10. A study to evaluate the safety, tolerability and efficacy of MHV370 in participants with Sjogren\u2019s Syndrome (SjS) or Mixed Connective Tissue Disease (MCTD):https:\/\/classic.clinicaltrials.gov\/ct2\/show\/NCT04988087<\/a><\/p>\n

 <\/p>\n

\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u7528\u306b\u306f\u4f7f\u7528\u3044\u305f\u3060\u3051\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

\u30b9\u30da\u30a4\u30f3\u306e7\u6b73\u5973\u5150\u304c\u767a\u75c7\u3057\u305f\u3001\u7570\u5e38\u306a\u5168\u8eab\u6027\u30a8\u30ea\u30c6\u30de\u30c8\u30fc\u30c7\u30b9\u3092\u8abf\u67fb\u3059\u308b\u305f\u3081\u306b\u3001\u4e16\u754c\u5404\u5730\u304b\u3089\u7814\u7a76\u8005\u30c1\u30fc\u30e0\u304c\u96c6\u7d50\u3057\u307e\u3057\u305f\u3002\u5168\u30b2\u30ce\u30e0\u30b7\u30fc\u30b1\u30f3\u30b9\u3067\u3001\u75c5\u6c17\u306e\u63a8\u5b9a\u539f\u56e0\u3068\u3057\u3066\u4e00\u3064\u306e\u907a\u4f1d\u5b50\u7570\u5e38\uff08TLR7\u907a\u4f1d\u5b50\u306e\u70b9\u5909\u7570\uff09\u304c\u5224\u660e\u3057\u307e\u3057\u305f\u3002\u3053\u306e<\/p>\n","protected":false},"author":975,"featured_media":17224,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[156],"tags":[183,184],"division":[],"class_list":{"0":"post-17222","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-mol-bio","8":"tag-sanger-seq","9":"tag-mutational-analysis","10":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"\n\u5168\u8eab\u6027\u30a8\u30ea\u30c6\u30de\u30c8\u30fc\u30c7\u30b9\u60a3\u8005\u306e\u7a81\u7136\u5909\u7570\u306e\u767a\u898b\u3068\u3001\u65b0\u305f\u306a\u6cbb\u7642\u6cd5\u306e\u958b\u62d3 - Learning at the Bench<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/sanger-seq-mutational-analysis-gsd-ts-ce-24029\/\" \/>\n<meta property=\"og:locale\" content=\"ja_JP\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"\u5168\u8eab\u6027\u30a8\u30ea\u30c6\u30de\u30c8\u30fc\u30c7\u30b9\u60a3\u8005\u306e\u7a81\u7136\u5909\u7570\u306e\u767a\u898b\u3068\u3001\u65b0\u305f\u306a\u6cbb\u7642\u6cd5\u306e\u958b\u62d3\" \/>\n<meta property=\"og:description\" content=\"\u30b9\u30da\u30a4\u30f3\u306e7\u6b73\u5973\u5150\u304c\u767a\u75c7\u3057\u305f\u3001\u7570\u5e38\u306a\u5168\u8eab\u6027\u30a8\u30ea\u30c6\u30de\u30c8\u30fc\u30c7\u30b9\u3092\u8abf\u67fb\u3059\u308b\u305f\u3081\u306b\u3001\u4e16\u754c\u5404\u5730\u304b\u3089\u7814\u7a76\u8005\u30c1\u30fc\u30e0\u304c\u96c6\u7d50\u3057\u307e\u3057\u305f\u3002\u5168\u30b2\u30ce\u30e0\u30b7\u30fc\u30b1\u30f3\u30b9\u3067\u3001\u75c5\u6c17\u306e\u63a8\u5b9a\u539f\u56e0\u3068\u3057\u3066\u4e00\u3064\u306e\u907a\u4f1d\u5b50\u7570\u5e38\uff08TLR7\u907a\u4f1d\u5b50\u306e\u70b9\u5909\u7570\uff09\u304c\u5224\u660e\u3057\u307e\u3057\u305f\u3002\u3053\u306e\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/sanger-seq-mutational-analysis-gsd-ts-ce-24029\/\" \/>\n<meta property=\"og:site_name\" content=\"Learning at the Bench\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/ThermoFisherJapan\" \/>\n<meta property=\"article:published_time\" content=\"2024-05-28T04:39:59+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2024-09-18T06:58:17+00:00\" \/>\n<meta property=\"og:image\" content=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2024\/05\/609584657b3e5cd663aa329ecd989088.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"2258\" \/>\n\t<meta property=\"og:image:height\" content=\"1262\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"LatB Staff\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@ThermoFisherJP\" \/>\n<meta name=\"twitter:site\" content=\"@ThermoFisherJP\" \/>\n<meta name=\"twitter:label1\" content=\"\u57f7\u7b46\u8005\" \/>\n\t<meta name=\"twitter:data1\" content=\"LatB Staff\" \/>\n\t<meta name=\"twitter:label2\" content=\"\u63a8\u5b9a\u8aad\u307f\u53d6\u308a\u6642\u9593\" \/>\n\t<meta name=\"twitter:data2\" content=\"15\u5206\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/sanger-seq-mutational-analysis-gsd-ts-ce-24029\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/sanger-seq-mutational-analysis-gsd-ts-ce-24029\\\/\"},\"author\":{\"name\":\"LatB Staff\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/#\\\/schema\\\/person\\\/bdbe319a2001f279ddce6ab8eaad1253\"},\"headline\":\"\u5168\u8eab\u6027\u30a8\u30ea\u30c6\u30de\u30c8\u30fc\u30c7\u30b9\u60a3\u8005\u306e\u7a81\u7136\u5909\u7570\u306e\u767a\u898b\u3068\u3001\u65b0\u305f\u306a\u6cbb\u7642\u6cd5\u306e\u958b\u62d3\",\"datePublished\":\"2024-05-28T04:39:59+00:00\",\"dateModified\":\"2024-09-18T06:58:17+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/sanger-seq-mutational-analysis-gsd-ts-ce-24029\\\/\"},\"wordCount\":560,\"commentCount\":0,\"image\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/sanger-seq-mutational-analysis-gsd-ts-ce-24029\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/wp-content\\\/uploads\\\/sites\\\/13\\\/2024\\\/05\\\/609584657b3e5cd663aa329ecd989088.jpg\",\"keywords\":[\"\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\",\"\u5909\u7570\u89e3\u6790\"],\"articleSection\":[\"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023\"],\"inLanguage\":\"ja\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/sanger-seq-mutational-analysis-gsd-ts-ce-24029\\\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/sanger-seq-mutational-analysis-gsd-ts-ce-24029\\\/\",\"url\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/sanger-seq-mutational-analysis-gsd-ts-ce-24029\\\/\",\"name\":\"\u5168\u8eab\u6027\u30a8\u30ea\u30c6\u30de\u30c8\u30fc\u30c7\u30b9\u60a3\u8005\u306e\u7a81\u7136\u5909\u7570\u306e\u767a\u898b\u3068\u3001\u65b0\u305f\u306a\u6cbb\u7642\u6cd5\u306e\u958b\u62d3 - Learning at the Bench\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/sanger-seq-mutational-analysis-gsd-ts-ce-24029\\\/#primaryimage\"},\"image\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/sanger-seq-mutational-analysis-gsd-ts-ce-24029\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/wp-content\\\/uploads\\\/sites\\\/13\\\/2024\\\/05\\\/609584657b3e5cd663aa329ecd989088.jpg\",\"datePublished\":\"2024-05-28T04:39:59+00:00\",\"dateModified\":\"2024-09-18T06:58:17+00:00\",\"author\":{\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/#\\\/schema\\\/person\\\/bdbe319a2001f279ddce6ab8eaad1253\"},\"breadcrumb\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/sanger-seq-mutational-analysis-gsd-ts-ce-24029\\\/#breadcrumb\"},\"inLanguage\":\"ja\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/sanger-seq-mutational-analysis-gsd-ts-ce-24029\\\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"ja\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/sanger-seq-mutational-analysis-gsd-ts-ce-24029\\\/#primaryimage\",\"url\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/wp-content\\\/uploads\\\/sites\\\/13\\\/2024\\\/05\\\/609584657b3e5cd663aa329ecd989088.jpg\",\"contentUrl\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/wp-content\\\/uploads\\\/sites\\\/13\\\/2024\\\/05\\\/609584657b3e5cd663aa329ecd989088.jpg\",\"width\":2258,\"height\":1262},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/sanger-seq-mutational-analysis-gsd-ts-ce-24029\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"\u5168\u8eab\u6027\u30a8\u30ea\u30c6\u30de\u30c8\u30fc\u30c7\u30b9\u60a3\u8005\u306e\u7a81\u7136\u5909\u7570\u306e\u767a\u898b\u3068\u3001\u65b0\u305f\u306a\u6cbb\u7642\u6cd5\u306e\u958b\u62d3\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/#website\",\"url\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/\",\"name\":\"Learning at the Bench\",\"description\":\"\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"ja\"},{\"@type\":\"Person\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/#\\\/schema\\\/person\\\/bdbe319a2001f279ddce6ab8eaad1253\",\"name\":\"LatB Staff\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"ja\",\"@id\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/2b3fdaccc53ad9dae08baccbcf9e8774350bd5682b89e353a9b7a999834e9306?s=96&d=mm&r=g\",\"url\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/2b3fdaccc53ad9dae08baccbcf9e8774350bd5682b89e353a9b7a999834e9306?s=96&d=mm&r=g\",\"contentUrl\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/2b3fdaccc53ad9dae08baccbcf9e8774350bd5682b89e353a9b7a999834e9306?s=96&d=mm&r=g\",\"caption\":\"LatB Staff\"},\"url\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/author\\\/lgbadmin\\\/\"}]}<\/script>\n<!-- \/ Yoast SEO Premium plugin. -->","yoast_head_json":{"title":"\u5168\u8eab\u6027\u30a8\u30ea\u30c6\u30de\u30c8\u30fc\u30c7\u30b9\u60a3\u8005\u306e\u7a81\u7136\u5909\u7570\u306e\u767a\u898b\u3068\u3001\u65b0\u305f\u306a\u6cbb\u7642\u6cd5\u306e\u958b\u62d3 - Learning at the Bench","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/sanger-seq-mutational-analysis-gsd-ts-ce-24029\/","og_locale":"ja_JP","og_type":"article","og_title":"\u5168\u8eab\u6027\u30a8\u30ea\u30c6\u30de\u30c8\u30fc\u30c7\u30b9\u60a3\u8005\u306e\u7a81\u7136\u5909\u7570\u306e\u767a\u898b\u3068\u3001\u65b0\u305f\u306a\u6cbb\u7642\u6cd5\u306e\u958b\u62d3","og_description":"\u30b9\u30da\u30a4\u30f3\u306e7\u6b73\u5973\u5150\u304c\u767a\u75c7\u3057\u305f\u3001\u7570\u5e38\u306a\u5168\u8eab\u6027\u30a8\u30ea\u30c6\u30de\u30c8\u30fc\u30c7\u30b9\u3092\u8abf\u67fb\u3059\u308b\u305f\u3081\u306b\u3001\u4e16\u754c\u5404\u5730\u304b\u3089\u7814\u7a76\u8005\u30c1\u30fc\u30e0\u304c\u96c6\u7d50\u3057\u307e\u3057\u305f\u3002\u5168\u30b2\u30ce\u30e0\u30b7\u30fc\u30b1\u30f3\u30b9\u3067\u3001\u75c5\u6c17\u306e\u63a8\u5b9a\u539f\u56e0\u3068\u3057\u3066\u4e00\u3064\u306e\u907a\u4f1d\u5b50\u7570\u5e38\uff08TLR7\u907a\u4f1d\u5b50\u306e\u70b9\u5909\u7570\uff09\u304c\u5224\u660e\u3057\u307e\u3057\u305f\u3002\u3053\u306e","og_url":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/sanger-seq-mutational-analysis-gsd-ts-ce-24029\/","og_site_name":"Learning at the Bench","article_publisher":"https:\/\/www.facebook.com\/ThermoFisherJapan","article_published_time":"2024-05-28T04:39:59+00:00","article_modified_time":"2024-09-18T06:58:17+00:00","og_image":[{"width":2258,"height":1262,"url":"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2024\/05\/609584657b3e5cd663aa329ecd989088.jpg","type":"image\/jpeg"}],"author":"LatB Staff","twitter_card":"summary_large_image","twitter_creator":"@ThermoFisherJP","twitter_site":"@ThermoFisherJP","twitter_misc":{"\u57f7\u7b46\u8005":"LatB Staff","\u63a8\u5b9a\u8aad\u307f\u53d6\u308a\u6642\u9593":"15\u5206"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/sanger-seq-mutational-analysis-gsd-ts-ce-24029\/#article","isPartOf":{"@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/sanger-seq-mutational-analysis-gsd-ts-ce-24029\/"},"author":{"name":"LatB Staff","@id":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/#\/schema\/person\/bdbe319a2001f279ddce6ab8eaad1253"},"headline":"\u5168\u8eab\u6027\u30a8\u30ea\u30c6\u30de\u30c8\u30fc\u30c7\u30b9\u60a3\u8005\u306e\u7a81\u7136\u5909\u7570\u306e\u767a\u898b\u3068\u3001\u65b0\u305f\u306a\u6cbb\u7642\u6cd5\u306e\u958b\u62d3","datePublished":"2024-05-28T04:39:59+00:00","dateModified":"2024-09-18T06:58:17+00:00","mainEntityOfPage":{"@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/sanger-seq-mutational-analysis-gsd-ts-ce-24029\/"},"wordCount":560,"commentCount":0,"image":{"@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/sanger-seq-mutational-analysis-gsd-ts-ce-24029\/#primaryimage"},"thumbnailUrl":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2024\/05\/609584657b3e5cd663aa329ecd989088.jpg","keywords":["\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9","\u5909\u7570\u89e3\u6790"],"articleSection":["\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023"],"inLanguage":"ja","potentialAction":[{"@type":"CommentAction","name":"Comment","target":["https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/sanger-seq-mutational-analysis-gsd-ts-ce-24029\/#respond"]}]},{"@type":"WebPage","@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/sanger-seq-mutational-analysis-gsd-ts-ce-24029\/","url":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/sanger-seq-mutational-analysis-gsd-ts-ce-24029\/","name":"\u5168\u8eab\u6027\u30a8\u30ea\u30c6\u30de\u30c8\u30fc\u30c7\u30b9\u60a3\u8005\u306e\u7a81\u7136\u5909\u7570\u306e\u767a\u898b\u3068\u3001\u65b0\u305f\u306a\u6cbb\u7642\u6cd5\u306e\u958b\u62d3 - Learning at the Bench","isPartOf":{"@id":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/#website"},"primaryImageOfPage":{"@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/sanger-seq-mutational-analysis-gsd-ts-ce-24029\/#primaryimage"},"image":{"@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/sanger-seq-mutational-analysis-gsd-ts-ce-24029\/#primaryimage"},"thumbnailUrl":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2024\/05\/609584657b3e5cd663aa329ecd989088.jpg","datePublished":"2024-05-28T04:39:59+00:00","dateModified":"2024-09-18T06:58:17+00:00","author":{"@id":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/#\/schema\/person\/bdbe319a2001f279ddce6ab8eaad1253"},"breadcrumb":{"@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/sanger-seq-mutational-analysis-gsd-ts-ce-24029\/#breadcrumb"},"inLanguage":"ja","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/sanger-seq-mutational-analysis-gsd-ts-ce-24029\/"]}]},{"@type":"ImageObject","inLanguage":"ja","@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/sanger-seq-mutational-analysis-gsd-ts-ce-24029\/#primaryimage","url":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2024\/05\/609584657b3e5cd663aa329ecd989088.jpg","contentUrl":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2024\/05\/609584657b3e5cd663aa329ecd989088.jpg","width":2258,"height":1262},{"@type":"BreadcrumbList","@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/sanger-seq-mutational-analysis-gsd-ts-ce-24029\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/"},{"@type":"ListItem","position":2,"name":"\u5168\u8eab\u6027\u30a8\u30ea\u30c6\u30de\u30c8\u30fc\u30c7\u30b9\u60a3\u8005\u306e\u7a81\u7136\u5909\u7570\u306e\u767a\u898b\u3068\u3001\u65b0\u305f\u306a\u6cbb\u7642\u6cd5\u306e\u958b\u62d3"}]},{"@type":"WebSite","@id":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/#website","url":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/","name":"Learning at the Bench","description":"","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"ja"},{"@type":"Person","@id":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/#\/schema\/person\/bdbe319a2001f279ddce6ab8eaad1253","name":"LatB Staff","image":{"@type":"ImageObject","inLanguage":"ja","@id":"https:\/\/secure.gravatar.com\/avatar\/2b3fdaccc53ad9dae08baccbcf9e8774350bd5682b89e353a9b7a999834e9306?s=96&d=mm&r=g","url":"https:\/\/secure.gravatar.com\/avatar\/2b3fdaccc53ad9dae08baccbcf9e8774350bd5682b89e353a9b7a999834e9306?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/2b3fdaccc53ad9dae08baccbcf9e8774350bd5682b89e353a9b7a999834e9306?s=96&d=mm&r=g","caption":"LatB Staff"},"url":"https:\/\/admin.acceleratingscience.com\/author\/lgbadmin\/"}]}},"taxonomy_info":{"category":[{"value":156,"label":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023"}],"post_tag":[{"value":183,"label":"\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9"},{"value":184,"label":"\u5909\u7570\u89e3\u6790"}]},"featured_image_src_large":["https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2024\/05\/609584657b3e5cd663aa329ecd989088-1024x572.jpg",760,425,true],"author_info":{"display_name":"LatB Staff","author_link":"https:\/\/admin.acceleratingscience.com\/author\/lgbadmin\/"},"comment_info":0,"category_info":[{"term_id":156,"name":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","slug":"mol-bio","term_group":0,"term_taxonomy_id":156,"taxonomy":"category","description":"","parent":0,"count":538,"filter":"raw","meta":[],"cat_ID":156,"category_count":538,"category_description":"","cat_name":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","category_nicename":"mol-bio","category_parent":0}],"tag_info":[{"term_id":183,"name":"\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9","slug":"sanger-seq","term_group":0,"term_taxonomy_id":183,"taxonomy":"post_tag","description":"","parent":0,"count":33,"filter":"raw","meta":[]},{"term_id":184,"name":"\u5909\u7570\u89e3\u6790","slug":"mutational-analysis","term_group":0,"term_taxonomy_id":184,"taxonomy":"post_tag","description":"","parent":0,"count":3,"filter":"raw","meta":[]}],"jetpack-related-posts":[],"jetpack_sharing_enabled":true,"amp_enabled":true,"jetpack_featured_media_url":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2024\/05\/609584657b3e5cd663aa329ecd989088.jpg","_links":{"self":[{"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/posts\/17222","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/users\/975"}],"replies":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/comments?post=17222"}],"version-history":[{"count":0,"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/posts\/17222\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/media\/17224"}],"wp:attachment":[{"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/media?parent=17222"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/categories?post=17222"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/tags?post=17222"},{"taxonomy":"division","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/division?post=17222"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}