{"id":18441,"date":"2024-11-15T15:28:20","date_gmt":"2024-11-15T06:28:20","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/?p=18441"},"modified":"2024-11-15T15:28:20","modified_gmt":"2024-11-15T06:28:20","slug":"mycoplasma-qpcr-3-bpg-24074","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/mycoplasma-qpcr-3-bpg-24074\/","title":{"rendered":"\u3010\u521d\u5fc3\u8005\u3067\u3082\u308f\u304b\u308b\uff01\u3011\u30de\u30a4\u30b3\u30d7\u30e9\u30ba\u30de\u5426\u5b9a\u8a66\u9a13\u30d0\u30ea\u30c7\u30fc\u30b7\u30e7\u30f3\u306e\u9032\u3081\u65b9"},"content":{"rendered":"

\u54c1\u8cea\u8a66\u9a13\u306b\u95a2\u3059\u308b\u5c02\u9580\u7528\u8a9e\u306f\u96e3\u3057\u304f\u3066\u3001\u3044\u307e\u3055\u3089\u805e\u3051\u306a\u3044\u3068\u56f0\u3063\u305f\u3053\u3068\u306f\u3042\u308a\u307e\u305b\u3093\u304b\uff1f\u3053\u306e\u30d6\u30ed\u30b0\u3067\u306f\u3001\u57fa\u790e\u7528\u8a9e\u3092\u5b66\u3076\u3068\u3068\u3082\u306b\u3001\u77e5\u3063\u3066\u304a\u304f\u3068\u4fbf\u5229\u306a\u60c5\u5831\u3092\u304a\u5c4a\u3051\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n

\u3055\u3066\u3001\u30de\u30a4\u30b3\u30d7\u30e9\u30ba\u30de\u5426\u5b9a\u8a66\u9a13\u306e\u30d0\u30ea\u30c7\u30fc\u30b7\u30e7\u30f3\u306b\u304a\u3044\u3066\u306f\u3001\u7279\u7570\u6027\u3001\u691c\u51fa\u611f\u5ea6\u3001\u9811\u5065\u6027\u304c\u975e\u5e38\u306b\u91cd\u8981\u306b\u306a\u308a\u307e\u3059\u3002\u3057\u304b\u3057\u3001\u306f\u3058\u3081\u3066\u306e\u5834\u5408\u3001\u3069\u306e\u3088\u3046\u306a\u6d41\u308c\u3067\u30d0\u30ea\u30c7\u30fc\u30b7\u30e7\u30f3\u3092\u5b9f\u65bd\u3057\u3066\u3044\u3051\u3070\u826f\u3044\u306e\u304b\u3001\u30a4\u30e1\u30fc\u30b8\u304c\u308f\u304b\u306a\u3044\u65b9\u3082\u591a\u3044\u306e\u3067\u306f\u306a\u3044\u3067\u3057\u3087\u3046\u304b\u3002\u305d\u3053\u3067\u4eca\u56de\u306f\u3001\u7121\u4e8b\u306b\u898f\u5236\u5f53\u5c40\u306b\u627f\u8a8d\u3055\u308c\u308b\u307e\u3067\u3001\u3069\u306e\u3088\u3046\u306b\u9032\u3081\u3066\u3044\u304f\u65b9\u6cd5\u304c\u3042\u308b\u306e\u304b\u7d39\u4ecb\u3057\u305f\u3044\u3068\u601d\u3044\u307e\u3059\u3002
\n\u627f\u8a8d\u307e\u3067\u306e\u6d41\u308c\u3092\u7406\u89e3\u3059\u308b\u3053\u3068\u3067\u3001\u5f53\u793e\u306e\u30d0\u30ea\u30c7\u30fc\u30b7\u30e7\u30f3\u6e08\u307f\u306e\u30ad\u30c3\u30c8\u304c\u3001\u3044\u304b\u306b\u52b9\u7387\u7684\u306a\u30d0\u30ea\u30c7\u30fc\u30b7\u30e7\u30f3\u306b\u5f79\u7acb\u3064\u304b\u3092\u77e5\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n

\u25bc\u3053\u3093\u306a\u65b9\u306b\u304a\u3059\u3059\u3081\u3067\u3059\uff01
\n\u30fb\u533b\u85ac\u54c1\u306e\u54c1\u8cea\u8a66\u9a13\u3067\u30de\u30a4\u30b3\u30d7\u30e9\u30ba\u30de\u5426\u5b9a\u8a66\u9a13\u3092\u5b9f\u65bd\u3059\u308b\u4e88\u5b9a\u306e\u65b9
\n\u30fb\u30de\u30a4\u30b3\u30d7\u30e9\u30ba\u30de\u5426\u5b9a\u8a66\u9a13\u306e\u30d0\u30ea\u30c7\u30fc\u30b7\u30e7\u30f3\u306b\u8208\u5473\u306e\u3042\u308b\u65b9<\/p>\n

\u30d0\u30ea\u30c7\u30fc\u30b7\u30e7\u30f3\u304c\u5fc5\u8981\u306a\u7406\u7531<\/h2>\n

\u533b\u85ac\u54c1\u306e\u81e8\u5e8a\u8a66\u9a13\u304a\u3088\u3073\u88fd\u9020\u8ca9\u58f2\u627f\u8a8d\u7533\u8acb\u306e\u305f\u3081\u306e\u30de\u30a4\u30b3\u30d7\u30e9\u30ba\u30de\u5426\u5b9a\u8a66\u9a13\u306e\u5834\u5408\u3001\u65e5\u672c\u85ac\u5c40\u65b9\u3067\u306f\u5f93\u6765\u6cd5\uff08A\u6cd5:\u57f9\u990a\u6cd5\u3001B\u6cd5:\u6307\u6a19\u7d30\u80de\u3092\u7528\u3044\u305fDNA\u67d3\u8272\u6cd5\uff09\u3068\u3001\u65b0\u305f\u306a\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u3068\u3057\u3066\u52a0\u308f\u3063\u305fC\u6cd5:\u6838\u9178\u5897\u5e45\u6cd5\u306e3\u3064\u306e\u624b\u6cd5\u304c\u3042\u308a\u307e\u3059\u3002
\n\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3092\u7528\u3044\u305f\u624b\u6cd5\u306fC\u6cd5:\u6838\u9178\u5897\u5e45\u6cd5\u306b\u306a\u308a\u307e\u3059\u304c\u3001\u5f93\u6765\u6cd5\u3068\u540c\u7b49\u306e\u7cbe\u5ea6\u3092\u793a\u3059\u305f\u3081\u306b\u30d0\u30ea\u30c7\u30fc\u30b7\u30e7\u30f3\u3092\u5b9f\u65bd\u3059\u308b\u3053\u3068\u304c\u8981\u6c42\u3055\u308c\u307e\u3059\u3002<\/p>\n\n\n\n\n\n\n\n
\u88681. \u65e5\u672c\u85ac\u5c40\u65b9\u306b\u304a\u3051\u308bC\u6cd5\u306e\u4f4d\u7f6e\u3065\u3051<\/b><\/caption>\n
\u7b2c18\u6539\u6b63\u65e5\u672c\u85ac\u5c40\u65b9<\/b><\/font><\/td>\n<\/tr>\n
\u8a66\u9a13\u6cd5<\/b><\/td>\n\u30fb\u57f9\u990a\u6cd5\uff08A\u6cd5\uff09
\u30fb\u6307\u6a19\u7d30\u80de\u3092\u7528\u3044\u305fDNA\u67d3\u8272\u6cd5\uff08B\u6cd5\uff09
\u30fb\u6838\u9178\u5897\u5e45\u6cd5\uff08C\u6cd5\uff09<\/td>\n<\/tr>\n
\u8a66\u9a13\u6cd5\u306e\u9078\u629e<\/b><\/td>\n\u30fbA\u6cd5\u3001B\u6cd5\u3092\u5b9f\u65bd\u3059\u308b\u3002
\u30fb\u9069\u5207\u306a\u30d0\u30ea\u30c7\u30fc\u30b7\u30e7\u30f3\u3092\u5b9f\u65bd\u3059\u308b\u3053\u3068\u3067\u3001
\u3000C\u6cd5\u3092A\u6cd5\u3001B\u6cd5\u306e\u4ee3\u66ff\u6cd5\u3068\u3057\u3066\u4f7f\u7528\u3059\u308b\u3002<\/b><\/font><\/td>\n<\/tr>\n
\u30d0\u30ea\u30c7\u30fc\u30b7\u30e7\u30f3\u306e
\u8a55\u4fa1\u9805\u76ee<\/b><\/td>\n		\u30fb\u7279\u7570\u6027
\u30fb\u691c\u51fa\u9650\u754c
  \u30fbA\u6cd5\u306e\u4ee3\u66ff\u6761\u4ef6\uff1a\u611f\u5ea610CFU\/mL
  \u30fbB\u6cd5\u306e\u4ee3\u66ff\u6761\u4ef6\uff1a\u611f\u5ea6100CFU\/mL
\u30fb\u9811\u5065\u6027<\/td>\n<\/tr>\n
\u691c\u51fa\u5bfe\u8c61<\/b><\/td>\n7\u83cc\u7a2e<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u8a66\u9a13\u65b9\u6cd5\u306e\u6700\u9069\u5316\u3068\u30d7\u30ec\u30d0\u30ea\u30c7\u30fc\u30b7\u30e7\u30f3<\/h2>\n

\u307e\u305a\u3001\u30d0\u30ea\u30c7\u30fc\u30b7\u30e7\u30f3\u306e\u524d\u6bb5\u968e\u3068\u3057\u3066\u3001\u8a66\u9a13\u65b9\u6cd5\u306e\u6700\u9069\u5316\u3068\u30d7\u30ec\u30d0\u30ea\u30c7\u30fc\u30b7\u30e7\u30f3\u30fb\u9069\u683c\u6027\u8a55\u4fa1\u3092\u5b9f\u65bd\u3057\u307e\u3059\u3002<\/p>\n

\u3010\u8a66\u9a13\u65b9\u6cd5\u306e\u6700\u9069\u5316\u3011
\nStep 1
\n\u304a\u5ba2\u3055\u307e\u306e\u30b5\u30f3\u30d7\u30eb\u3092\u4f7f\u3063\u3066\u3001\u63a8\u5968\u306e\u30d7\u30ed\u30c8\u30b3\u30eb\u3067\u554f\u984c\u306a\u304f\u8a66\u9a13\u304c\u3067\u304d\u308b\u304b\u78ba\u8a8d\u3057\u307e\u3059\u3002\u3053\u306e\u6642\u3001\u65e5\u672c\u85ac\u5c40\u65b9\u306b\u8a18\u8f09\u3055\u308c\u3066\u3044\u308b\u611f\u5ea6\u3092\u6e80\u305f\u305b\u308b\u304b\u3001\u30b5\u30f3\u30d7\u30eb\uff0bDPC\uff08\u30ad\u30c3\u30c8\u306b\u4ed8\u5c5e\u306e\u30dd\u30b8\u30c6\u30a3\u30d6\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\uff09\u30b9\u30d1\u30a4\u30af\u8a66\u9a13\u3067\u300110 GC\/mL\u306e\u611f\u5ea6\u3092\u9054\u6210\u3067\u304d\u308b\u304b\u3092\u78ba\u8a8d\u3057\u307e\u3059\u3002<\/p>\n

Step 2
\n\u30b5\u30f3\u30d7\u30ea\u30f3\u30b0\u30dd\u30a4\u30f3\u30c8\u3092\u6c7a\u5b9a\u3057\u307e\u3059\u3002\u7d30\u80de\u306e\u30cf\u30fc\u30d9\u30b9\u30c8\u6bb5\u968e\u3060\u3051\u3067\u8a66\u9a13\u3059\u308b\u306e\u3067\u306f\u306a\u304f\u3001\u57f9\u990a\u9014\u4e2d\u306e\u4e0a\u6e05\u306a\u3069\u3092\u4f7f\u3063\u3066\u5de5\u7a0b\u5185\u8a66\u9a13\u3092\u884c\u3046\u3068\u3001\u30de\u30a4\u30b3\u30d7\u30e9\u30ba\u30de\u6c5a\u67d3\u306e\u30ea\u30b9\u30af\u3092\u3088\u308a\u8efd\u6e1b\u3067\u304d\u307e\u3059\u3002<\/p>\n

\u3010\u30d7\u30ec\u30d0\u30ea\u30c7\u30fc\u30b7\u30e7\u30f3\u3011
\n\u30de\u30a4\u30b3\u30d7\u30e9\u30ba\u30de\u30b2\u30ce\u30e0DNA\u3092\u7528\u3044\u3066\u4e88\u5099\u5b9f\u9a13\u3092\u884c\u3044\u307e\u3059\u3002
\nStep 1\u3068\u540c\u69d8\u306b\u3001\u65e5\u672c\u85ac\u5c40\u6cd5\u306b\u8a18\u8f09\u3055\u308c\u3066\u3044\u308b\u611f\u5ea6\u3092\u6e80\u305f\u3059\u3053\u3068\u304c\u3067\u304d\u308b\u304b\u3001\u30b5\u30f3\u30d7\u30eb\uff0b\u30de\u30a4\u30b3\u30d7\u30e9\u30ba\u30de\u30b2\u30ce\u30e0DNA\u30b9\u30d1\u30a4\u30af\u8a66\u9a13\u3067\u300110 GC\/mL\u306e\u611f\u5ea6\u3092\u9054\u6210\u3067\u304d\u308b\u304b\u3092\u78ba\u8a8d\u3057\u307e\u3059\u3002
\n

\"\u56f31.

\u56f31. \u4e00\u822c\u7684\u306a\u30d0\u30ea\u30c7\u30fc\u30b7\u30e7\u30f3\u30d5\u30ed\u30fc<\/p><\/div><\/p>\n

\u30d0\u30ea\u30c7\u30fc\u30b7\u30e7\u30f3\u5b9f\u65bd\u3068\u627f\u8a8d\u7533\u8acb<\/h2>\n

\u3010\u30d0\u30ea\u30c7\u30fc\u30b7\u30e7\u30f3\u5b9f\u65bd\u3011
\nStep 1
\n\u30d7\u30ec\u30d0\u30ea\u30c7\u30fc\u30b7\u30e7\u30f3\u306e\u7d50\u679c\u3092\u3082\u3068\u306b\u3001\u898f\u5236\u5f53\u5c40\u3068\u4e8b\u524d\u9762\u8ac7\u3057\u3001\u5b9f\u969b\u306e\u30d0\u30ea\u30c7\u30fc\u30b7\u30e7\u30f3\u5185\u5bb9\u3092\u76f8\u8ac7\u3057\u307e\u3059\u3002\u304a\u5ba2\u3055\u307e\u306e\u30b5\u30f3\u30d7\u30eb\u3092\u7528\u3044\u3066\u30d0\u30ea\u30c7\u30fc\u30b7\u30e7\u30f3\u3092\u5b9f\u65bd\u3059\u308b\u5fc5\u8981\u306f\u3042\u308a\u307e\u3059\u304c\u3001\u5f53\u793e\u306e\u30ad\u30c3\u30c8\u3092\u3054\u4f7f\u7528\u306e\u5834\u5408\u3001\u53c2\u8003\u8cc7\u6599\u3068\u3057\u3066\u5f53\u793e\u304c\u53d6\u5f97\u3057\u305f\u30d0\u30ea\u30c7\u30fc\u30b7\u30e7\u30f3\u30ec\u30dd\u30fc\u30c8\u3092\u63d0\u4f9b\u3067\u304d\u307e\u3059\u306e\u3067\u3001\u304a\u5ba2\u3055\u307e\u81ea\u8eab\u304c1\u304b\u3089\u30d0\u30ea\u30c7\u30fc\u30b7\u30e7\u30f3\u3092\u5b9f\u65bd\u3059\u308b\u5fc5\u8981\u306f\u3042\u308a\u307e\u305b\u3093\u3002<\/p>\n

Step.2
\n\u898f\u5236\u5f53\u5c40\u3068\u306e\u9762\u8ac7\u7d50\u679c\u3092\u8e0f\u307e\u3048\u305f\u30d7\u30e9\u30f3\u306b\u5f93\u3044\u3001\u30d0\u30ea\u30c7\u30fc\u30b7\u30e7\u30f3\u3092\u5b9f\u65bd\u3057\u307e\u3059\u3002<\/p>\n

\u3010\u898f\u5236\u5f53\u5c40\u3078\u306e\u627f\u8a8d\u7533\u8acb\u3011
\nStep.1
\n\u30d0\u30ea\u30c7\u30fc\u30b7\u30e7\u30f3\u7d50\u679c\u3092\u3082\u3068\u306b\u898f\u5236\u5f53\u5c40\u306b\u533b\u85ac\u54c1\u306e\u627f\u8a8d\u7533\u8acb\u3092\u884c\u3044\u307e\u3059\u3002<\/p>\n

Step.2
\n\u627f\u8a8d\u5f8c\u3001\u30d0\u30ea\u30c7\u30fc\u30b7\u30e7\u30f3\u3092\u3068\u3063\u305f\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\uff08SOP\uff09\u306b\u5f93\u3063\u3066\u3001\u30de\u30a4\u30b3\u30d7\u30e9\u30ba\u30de\u5426\u5b9a\u8a66\u9a13\u3092\u5b9f\u65bd\u3057\u3066\u3044\u304d\u307e\u3059\u3002
\n\"\"<\/p>\n

\u307e\u3068\u3081<\/h2>\n

\u3044\u304b\u304c\u3067\u3057\u305f\u304b\uff1f
\n\u5b89\u5168\u306a\u533b\u85ac\u54c1\u3092\u63d0\u4f9b\u3059\u308b\u305f\u3081\u306b\u306f\u3001\u304d\u3061\u3093\u3068\u30d0\u30ea\u30c7\u30fc\u30c8\u3055\u308c\u305f\u65b9\u6cd5\u3067\u8a66\u9a13\u3092\u5b9f\u65bd\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u304c\u3001\u30d0\u30ea\u30c7\u30fc\u30b7\u30e7\u30f3\u306f\u624b\u9593\u3068\u6642\u9593\u306e\u304b\u304b\u308b\u5927\u5909\u306a\u4f5c\u696d\u3067\u3059\u3002\u305d\u3053\u3067\u3001\u30d0\u30ea\u30c7\u30fc\u30b7\u30e7\u30f3\u6e08\u307f\u306e\u30ad\u30c3\u30c8\u3092\u4f7f\u7528\u3059\u308c\u3070\u3001\u898f\u5236\u5f53\u5c40\u3068\u306e\u9762\u8ac7\u6642\u306b\u53c2\u8003\u8cc7\u6599\u3068\u3057\u3066\u3001\u5f53\u793e\u304c\u53d6\u5f97\u3057\u305f\u30d0\u30ea\u30c7\u30fc\u30b7\u30e7\u30f3\u30ec\u30dd\u30fc\u30c8\u3092\u63d0\u51fa\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u3001\u304a\u5ba2\u3055\u307e\u81ea\u8eab\u30671\u304b\u3089\u30d0\u30ea\u30c7\u30fc\u30b7\u30e7\u30f3\u8a66\u9a13\u3092\u3059\u308b\u5fc5\u8981\u304c\u306a\u3044\u305f\u3081\u4f5c\u696d\u306b\u304b\u304b\u308b\u8ca0\u62c5\u304c\u8efd\u6e1b\u3055\u308c\u307e\u3059\u3002<\/p>\n

Applied Biosystems\u2122 MycoSEQ\u2122 Plus Mycoplasma Detection Kit\u306f\u3001\u7d30\u80de\u6cbb\u7642\u3084\u8907\u96d1\u306a\u30d0\u30a4\u30aa\u30d7\u30ed\u30c0\u30af\u30b7\u30e7\u30f3\u30b5\u30f3\u30d7\u30eb\u306b\u304a\u3051\u308b\u30de\u30a4\u30b3\u30d7\u30e9\u30ba\u30de\u306e\u691c\u51fa\u306b\u7279\u5316\u3057\u3066\u958b\u767a\u3055\u308c\u305fTaqMan\u2122\u30d9\u30fc\u30b9\u306e\u30ad\u30c3\u30c8\u3067\u3001\u898f\u5236\u3067\u8981\u6c42\u3055\u308c\u308b10 CFU\/mL\u304b\u305d\u308c\u4ee5\u4e0a\u306e\u9ad8\u3044\u611f\u5ea6\u3068\u7279\u7570\u6027\u3092\u6301\u3061\u3001\u88fd\u54c1\u306e\u30ed\u30c3\u30c8\u30ea\u30ea\u30fc\u30b9\u8a66\u9a13\u306b\u5bfe\u5fdc\u3059\u308b\u306e\u306b\u5fc5\u8981\u306a\u6027\u80fd\u3092\u5099\u3048\u3066\u3044\u307e\u3059\u3002\u305c\u3072\u3001\u30dd\u30b9\u30bf\u30fc\u3092\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3057\u3066\u3001\u3053\u306e\u88fd\u54c1\u306e\u8a73\u3057\u3044\u6027\u80fd\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/p>\n

\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u306f\u3053\u3061\u3089<\/a><\/p>\n

 <\/p>\n

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