{"id":186,"date":"2015-11-26T00:00:00","date_gmt":"2015-11-26T00:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/selection-antibiotics\/"},"modified":"2025-02-21T11:19:03","modified_gmt":"2025-02-21T02:19:03","slug":"selection-antibiotics","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/selection-antibiotics\/","title":{"rendered":"\u5b89\u5b9a\u306a\u5f62\u8cea\u8ee2\u63db\u4f53\u3092\u9078\u629e\u3059\u308b\u305f\u3081\u306e\u771f\u6838\u751f\u7269\u7528\u6297\u751f\u7269\u8cea\u307e\u3068\u3081"},"content":{"rendered":"

\u5b89\u5b9a\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u3092\u6210\u529f\u3055\u305b\u308b\u305f\u3081\u306b\u306f\u3001\u52b9\u7387\u7684\u306aDNA\u5c0e\u5165\u3068\u305d\u306eDNA\u3092\u53d6\u308a\u8fbc\u3093\u3060\u7d30\u80de\u3092\u9078\u629e\u3059\u308b\u65b9\u6cd5\u304c\u5fc5\u8981\u3068\u3055\u308c\u307e\u3059\u3002<\/strong>DNA\u304c\u5b89\u5b9a\u7684\u306b\u7d44\u307f\u8fbc\u307e\u308c\u308b\u306e\u306f104<\/sup>\u500b\u306e\u5c0e\u5165\u7d30\u80de\u306e\u3046\u3061\u7d041\u500b\u3067\u3059\u304c\u3001\u305d\u306e\u52b9\u7387\u306f\u7d30\u80de\u30bf\u30a4\u30d7\u3084\u7528\u3044\u3089\u308c\u308bDNA\u304c\u76f4\u9396\u72b6\u304b\u74b0\u72b6\u304b\u306b\u3088\u3063\u3066\u5909\u5316\u3057\u307e\u3059\u3002\u76f4\u9396\u72b6DNA\u304c\u7528\u3044\u3089\u308c\u305f\u6642\u306b\u3001\u7d44\u307f\u8fbc\u307f\u306f\u6700\u3082\u52b9\u7387\u7684\u3067\u3059\u3002<\/p>\n

\u5c0e\u5165\u3055\u308c\u305fDNA\u304c\u5b89\u5b9a\u7684\u306b\u767a\u73fe\u3055\u308c\u3066\u3044\u308b\u7d30\u80de\u3092\u9078\u629e\u3059\u308b\u305f\u3081\u306e\u6700\u3082\u4fe1\u983c\u3067\u304d\u308b\u65b9\u6cd5<\/strong>\u306e1\u3064\u306f\u3001\u5c0e\u5165\u306b\u7528\u3044\u3089\u308c\u308bDNA\u30b3\u30f3\u30b9\u30c8\u30e9\u30af\u30c8\u4e0a\u307e\u305f\u306f\u7d30\u80de\u306b\u5171\u5c0e\u5165\u3055\u308c\u308b\u5225\u500b\u306e\u30d9\u30af\u30bf\u30fc\u4e0a\u306b\u9078\u629e\u30de\u30fc\u30ab\u30fc\u3092\u542b\u307e\u305b\u3066\u3001\u77ed\u3044\u56de\u5fa9\u671f\u9593\u5f8c\u306b\u7d30\u80de\u306b\u9069\u5207\u306a\u9078\u629e\u5727\u3092\u304b\u3051\u308b\u3082\u306e\u3067\u3059\u3002\u5171\u5c0e\u5165\u3055\u308c\u305f\u30d9\u30af\u30bf\u30fc\u304b\u3089\u9078\u629e\u30de\u30fc\u30ab\u30fc\u304c\u767a\u73fe\u3055\u308c\u308b\u5834\u5408\u3001\u76ee\u7684\u306e\u907a\u4f1d\u5b50\u3092\u6301\u3064\u30d9\u30af\u30bf\u30fc\u3068\u9078\u629e\u30de\u30fc\u30ab\u30fc\u3092\u6301\u3064\u30d9\u30af\u30bf\u30fc\u306e\u30e2\u30eb\u6bd4\u306f\u30015\uff1a1\u304b\u308910\uff1a1\u306e\u7bc4\u56f2\u3068\u3057\u3001\u5168\u3066\u306e\u7d30\u80de\u306b\u9078\u629e\u30de\u30fc\u30ab\u30fc\u306b\u52a0\u3048\u3066\u76ee\u7684\u907a\u4f1d\u5b50\u3082\u78ba\u5b9f\u306b\u542b\u307e\u308c\u308b\u3088\u3046\u306b\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n

\u983b\u7e41\u306b\u4f7f\u7528\u3055\u308c\u308b\u9078\u629e\u30de\u30fc\u30ab\u30fc\u306f\u3001\u69d8\u3005\u306a\u9078\u629e\u8a66\u85ac\u306b\u5bfe\u3059\u308b\u8010\u6027\u3092\u4e0e\u3048\u308b\u907a\u4f1d\u5b50\u3001\u3042\u308b\u3044\u306f\u5c0e\u5165\u3055\u308c\u308b\u7d30\u80de\u7cfb\u306b\u304a\u3044\u3066\u6b20\u640d\u3057\u3066\u3044\u308b\u5fc5\u9808\u907a\u4f1d\u5b50\u3092\u88dc\u3046\u907a\u4f1d\u5b50\u3067\u3059\u3002\u9078\u629e\u57f9\u5730\u3067\u57f9\u990a\u3059\u308b\u3068\u3001\u5c0e\u5165\u3055\u308c\u3066\u3044\u306a\u3044\u7d30\u80de\u3042\u308b\u3044\u306f\u4e00\u904e\u6027\u5c0e\u5165\u3055\u308c\u305f\u7d30\u80de\u306f\u6700\u7d42\u7684\u306b\u6b7b\u6ec5\u3057\u3001\u6297\u751f\u7269\u8cea\u8010\u6027\u907a\u4f1d\u5b50\u3092\u5341\u5206\u306a\u30ec\u30d9\u30eb\u3067\u767a\u73fe\u3057\u3066\u3044\u308b\u7d30\u80de\u3042\u308b\u3044\u306f\u5fc5\u9808\u907a\u4f1d\u5b50\u306e\u6b20\u5931\u304c\u4ee3\u511f\u3055\u308c\u3066\u3044\u308b\u7d30\u80de\u306f\u751f\u5b58\u3057\u307e\u3059\u3002<\/p>\n

\u771f\u6838\u751f\u7269\u306e\u305f\u3081\u306e\u9078\u629e\u7528\u6297\u751f\u7269\u8cea<\/h2>\n

\u5f0a\u793e\u3067\u306f\u3001\u5e45\u5e83\u3044\u7a2e\u985e\u306e\u9078\u629e\u53ef\u80fd\u306a\u771f\u6838\u751f\u7269\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u306b\u5bfe\u5fdc\u3059\u308b\u9ad8\u54c1\u8cea\u306e\u9078\u629e\u7528\u8a66\u85ac\u3092\u63d0\u4f9b\u3057\u3066\u3044\u307e\u3059\u3002Gibco\u2122 Geneticin\u2122\uff08G418\u786b\u9178\u5869\uff09<\/a>\u3001Gibco\u2122 Zeocin\u2122<\/a>\u3001\u30cf\u30a4\u30b0\u30ed\u30de\u30a4\u30b7\u30f3B<\/a>\u3001\u30d4\u30e5\u30fc\u30ed\u30de\u30a4\u30b7\u30f3<\/a>\u3001\u304a\u3088\u3073\u30d6\u30e9\u30b9\u30c8\u30b5\u30a4\u30b8\u30f3<\/a>\u306e\u6297\u751f\u7269\u8cea\u304c\u5b89\u5b9a\u7d30\u80de\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306e\u305f\u3081\u306e\u9078\u629e\u7528\u6297\u751f\u7269\u8cea\u3068\u3057\u3066\u6700\u3082\u4e00\u822c\u7684\u306b\u7528\u3044\u3089\u308c\u3066\u3044\u307e\u3059\u3002\u3053\u308c\u3089\u306e\u6297\u751f\u7269\u8cea\u306f\u3001\u4e8c\u91cd\u9078\u629e\u304a\u3088\u3073\u8fc5\u901f\u306a\u5b89\u5b9a\u7d30\u80de\u7cfb\u6a39\u7acb\u306a\u3069\u306e\u7814\u7a76\u30cb\u30fc\u30ba\u306e\u305f\u3081\u306e\u30e6\u30cb\u30fc\u30af\u306a\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u3068\u3057\u3066\u63d0\u4f9b\u3055\u308c\u307e\u3059\u3002<\/p>\n

Geneticin \u9078\u629e\u7528\u6297\u751f\u7269\u8cea<\/a><\/h3>\n

Geneticin\u8a66\u85ac<\/strong>\u306f\u3001G418\u786b\u9178\u5869\u3068\u3057\u3066\u77e5\u3089\u308c\u3001\u54fa\u4e73\u985e\u3001\u690d\u7269\u3001\u307e\u305f\u306f\u9175\u6bcd\u7d30\u80de\u306e\u9078\u629e\u306b\u7528\u3044\u3089\u308c\u307e\u3059\u3002\u5f0a\u793e\u306eGeneticin\u8a66\u85ac\u306f\u9ad8\u7d14\u5ea6\u3067\u3042\u308b\u3053\u3068\u304b\u3089\u3001\u4ed6\u793e\u306eG418\u88fd\u54c1\u3088\u308a15\uff5e30%\u4f4e\u3044\u6fc3\u5ea6\u3067\u540c\u7b49\u306e\u9078\u629e\u80fd\u3092\u767a\u63ee\u3059\u308b\u305f\u3081\u3001\u751f\u5b58\u30af\u30ed\u30fc\u30f3\u30b3\u30ed\u30cb\u30fc\u304c\u3088\u308a\u65e9\u304f\u751f\u3058\u3001\u5916\u89b3\u304c\u3088\u308a\u5065\u5eb7\u306a\u7d30\u80de\u304c\u5f97\u3089\u308c\u308b\u3053\u3068\u304c\u671f\u5f85\u3067\u304d\u307e\u3059\u3002<\/p>\n

Zeocin \u9078\u629e\u7528\u6297\u751f\u7269\u8cea<\/a><\/h3>\n

Zeocin\u8a66\u85ac<\/strong>\u306f\u3001\u54fa\u4e73\u985e\u7d30\u80de\u7cfb\u3001\u9175\u6bcd\u3001\u6606\u866b\u7d30\u80de\u3001\u304a\u3088\u3073\u7d30\u83cc\u306b\u5bfe\u3059\u308b\u9078\u629e\u80fd\u304c\u3042\u308a\u307e\u3059\u3002Zeocin\u8a66\u85ac\u8010\u6027\u306f\u3001She ble<\/i>\u907a\u4f1d\u5b50\u306b\u3088\u3063\u3066\u4ed8\u4e0e\u3055\u308c\u307e\u3059\u3002\u7d30\u80de\u5185\u3067She ble<\/i>\u907a\u4f1d\u5b50\u306b\u3088\u3063\u3066\u767a\u73fe\u3055\u308c\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u306f\u3001Zeocin\u8a66\u85ac\u306b\u7d50\u5408\u3057\u3066\u305d\u306eDNA\u3078\u306e\u7d50\u5408\u3092\u963b\u5bb3\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u7d30\u80de\u5185DNA\u306e\u5207\u65ad\u3092\u59a8\u3052\u3001\u7d30\u80de\u6b7b\u3092\u963b\u6b62\u3057\u307e\u3059\u3002\u7d30\u80de\u306e\u9078\u629e\u306b\u5fc5\u8981\u3068\u3055\u308c\u308b\u6fc3\u5ea6\u7bc4\u56f2\u306f\u300150\uff5e2,000 \u03bcg\/mL\uff08\u4e00\u822c\u7684\u306b\u306f300\u03bcg\/mL\uff09\u3067\u3001\u7d30\u80de\u7a2e\u306b\u3088\u3063\u3066\u7570\u306a\u308a\u307e\u3059\u3002<\/p>\n

\u30cf\u30a4\u30b0\u30ed\u30de\u30a4\u30b7\u30f3B \u9078\u629e\u7528\u6297\u751f\u7269\u8cea<\/a><\/h3>\n

\u30cf\u30a4\u30b0\u30ed\u30de\u30a4\u30b7\u30f3B<\/strong>\u306f\u300180S\u30ea\u30dc\u30bd\u30fc\u30e0\u3067\u30c8\u30e9\u30f3\u30b9\u30ed\u30b1\u30fc\u30b7\u30e7\u30f3\u3092\u963b\u5bb3\u3057\u3001\u30df\u30b9\u30c8\u30e9\u30f3\u30b9\u30ec\u30fc\u30b7\u30e7\u30f3\u3092\u4fc3\u9032\u3059\u308b\u3053\u3068\u3067\u30bf\u30f3\u30d1\u30af\u8cea\u5408\u6210\u3092\u963b\u5bb3\u3059\u308b\u30a2\u30df\u30ce\u30b0\u30ea\u30b3\u30b7\u30c9\u7cfb\u6297\u751f\u7269\u8cea\u3067\u3059\u3002\u30cf\u30a4\u30b0\u30ed\u30de\u30a4\u30b7\u30f3B\u306e\u4f5c\u7528\u6a5f\u5e8f\u306f\u3001Geneticin \u8a66\u85ac\u304a\u3088\u3073Zeocin \u8a66\u85ac\u3068\u306f\u7570\u306a\u308b\u305f\u3081\u3001\u4ed6\u306e\u9078\u629e\u8a66\u85ac\u3068\u306e\u4f75\u7528\u30672\u91cd\u9078\u629e\u304c\u53ef\u80fd\u3067\u3059\u3002\u30cf\u30a4\u30b0\u30ed\u30de\u30a4\u30b7\u30f3B\u8010\u6027\u306f\u3001E. coli<\/i>\u7531\u6765\u306e\u30cf\u30a4\u30b0\u30ed\u30de\u30a4\u30b7\u30f3\u8010\u6027\u907a\u4f1d\u5b50\uff08hyg<\/i>\u307e\u305f\u306fhph<\/i>\uff09\u306b\u3088\u3063\u3066\u4ed8\u4e0e\u3055\u308c\u307e\u3059\u3002\u4f7f\u7528\u6fc3\u5ea6\u7bc4\u56f2\u306f\u3001100\uff5e1,000 \u03bcg\/mL\uff08\u4e00\u822c\u7684\u306b\u306f200 \u03bcg\/mL\uff09\u3067\u3001\u5404\u7d30\u80de\u7cfb\u306b\u5408\u308f\u305b\u3066\u6700\u9069\u5316\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n

\u30d4\u30e5\u30fc\u30ed\u30de\u30a4\u30b7\u30f3\u4e8c\u5869\u9178\u5869\u9078\u629e\u7528\u6297\u751f\u7269\u8cea<\/a><\/h3>\n

\u30d4\u30e5\u2015\u30ed\u30de\u30a4\u30b7\u30f3<\/strong>\u306f\u3001\u771f\u6838\u751f\u7269\u304a\u3088\u3073\u539f\u6838\u751f\u7269\u306e\u7ffb\u8a33\u3092\u963b\u5bb3\u3059\u308b\u3001Streptomyces alboniger<\/i>\u7531\u6765\u306e\u30a2\u30df\u30ce\u30cc\u30af\u30ec\u30aa\u30b7\u30c9\u7cfb\u6297\u751f\u7269\u8cea\u3067\u3059\u3002Streptomyces<\/i>\u7531\u6765\u306e\u30d4\u30e5\u2015\u30ed\u30de\u30a4\u30b7\u30f3N-\u30a2\u30bb\u30c1\u30eb\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30e9\u30fc\u30bc\u907a\u4f1d\u5b50\uff08pac<\/i>\uff09\u306b\u3088\u3063\u3066\u8010\u6027\u304c\u4ed8\u4e0e\u3055\u308c\u307e\u3059\u3002\u54fa\u4e73\u985e\u306e\u63a5\u7740\u7d30\u80de\u306f2\uff5e5 \u03bcg\/mL\u306e\u6fc3\u5ea6\u306b\u611f\u53d7\u6027\u3092\u793a\u3059\u4e00\u65b9\u3001\u6d6e\u904a\u7d30\u80de\u306f0.5\uff5e2 \u03bcg\/mL\u306e\u4f4e\u6fc3\u5ea6\u306b\u611f\u53d7\u6027\u3092\u793a\u3057\u307e\u3059\u3002\u4f5c\u7528\u6a5f\u5e8f\u306f\u8fc5\u901f\u3067\u3001\u4f4e\u6fc3\u5ea6\u3067\u901f\u3084\u304b\u306b\u7d30\u80de\u304c\u6b7b\u6ec5\u3059\u308b\u305f\u3081\u3001\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u5f8c1\u9031\u9593\u4ee5\u5185\u306b\u30d4\u30e5\u2015\u30ed\u30de\u30a4\u30b7\u30f3\u8010\u6027\u5b89\u5b9a\u7d30\u80de\u7cfb\u304c\u5f97\u3089\u308c\u307e\u3059\u3002<\/p>\n

\u30d6\u30e9\u30b9\u30c8\u30b5\u30a4\u30b8\u30f3S HCl \u9078\u629e\u7528\u6297\u751f\u7269\u8cea<\/a><\/h3>\n

\u30d6\u30e9\u30b9\u30c8\u30b5\u30a4\u30b8\u30f3<\/strong>\u306f\u3001\u771f\u6838\u751f\u7269\u304a\u3088\u3073\u539f\u6838\u751f\u7269\u306e\u7ffb\u8a33\u3092\u963b\u5bb3\u3059\u308b\u3001Streptomyces griseochromogenes<\/i>\u7531\u6765\u306e\u30cc\u30af\u30ec\u30aa\u30b7\u30c9\u7cfb\u6297\u751f\u7269\u8cea\u3067\u3059\u3002Aspergillus terreus<\/i>\u7531\u6765\u306ebsd<\/i>\u907a\u4f1d\u5b50\u306b\u3088\u3063\u3066\u8010\u6027\u304c\u4ed8\u4e0e\u3055\u308c\u307e\u3059\u3002E. coli<\/i>\u682a\u306f50 \u03bcg\/mL\u306e\u6fc3\u5ea6\u306b\u611f\u53d7\u6027\u3092\u793a\u3059\u4e00\u65b9\u3001\u54fa\u4e73\u985e\u7d30\u80de\u306f2\uff5e10 \u03bcg\/mL\u306e\u4f4e\u6fc3\u5ea6\u306b\u611f\u53d7\u6027\u3092\u793a\u3057\u307e\u3059\u3002\u901f\u3084\u304b\u306b\u7d30\u80de\u304c\u6b7b\u6ec5\u3059\u308b\u305f\u3081\u3001\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u5f8c1\u9031\u9593\u4ee5\u5185\u306b\u30d6\u30e9\u30b9\u30c8\u30b5\u30a4\u30b8\u30f3\u8010\u6027\u5b89\u5b9a\u54fa\u4e73\u985e\u7d30\u80de\u7cfb\u304c\u5f97\u3089\u308c\u307e\u3059\u3002<\/p>\n

\u3010\u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3011Gibco\u7d30\u80de\u57f9\u990a\u57fa\u790e\u30cf\u30f3\u30c9\u30d6\u30c3\u30af<\/h2>\n

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\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9<\/a><\/p>\n

 <\/p>\n

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