{"id":18677,"date":"2024-12-17T16:42:23","date_gmt":"2024-12-17T07:42:23","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/?p=18677"},"modified":"2024-12-17T16:42:23","modified_gmt":"2024-12-17T07:42:23","slug":"sanger-seq-ce-4-gsd-ts-ce-24086","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/sanger-seq-ce-4-gsd-ts-ce-24086\/","title":{"rendered":"\u30b7\u30fc\u30b1\u30f3\u30b9\u89e3\u6790\u306e\u30c8\u30e9\u30d6\u30eb\u30b7\u30e5\u30fc\u30c8\uff1a\u30b7\u30b0\u30ca\u30eb\u306e\u30c9\u30ed\u30c3\u30d7\u3068\u30c8\u30c3\u30d7\u30d8\u30d3\u30fc\u30c7\u30fc\u30bf"},"content":{"rendered":"

\u30b7\u30fc\u30b1\u30f3\u30b9\u89e3\u6790\u306b\u3088\u308a\u89e3\u8aad\u3067\u304d\u305f\u9577\u3055\u304c\u4e88\u60f3\u3088\u308a\u77ed\u3044\u5834\u5408\u3001\u3055\u307e\u3056\u307e\u306a\u539f\u56e0\u304c\u8003\u3048\u3089\u308c\u307e\u3059\u3002\u3088\u308a\u9577\u304f\u89e3\u8aad\u3059\u308b\u305f\u3081\u306e\u5bfe\u51e6\u6cd5\u306f\u3001\u539f\u56e0\u306b\u3088\u308a\u7570\u306a\u308b\u305f\u3081\u3001\u307e\u305aRaw Data\u3092\u78ba\u8a8d\u3057\u3001\u539f\u56e0\u3092\u8003\u5bdf\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002Applied Biosystems\u2122 Sequencing Analysis\u30bd\u30d5\u30c8\u30a6\u30a8\u30a2\u3067\u306eRaw Data\u306e\u78ba\u8a8d\u624b\u9806\u306f\u3001\u3053\u3061\u3089<\/a>\u3092\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002
\n\u672c\u30d6\u30ed\u30b0\u3067\u306f\u3001Raw Data\u306e\u30b7\u30b0\u30ca\u30eb\u304c\u4e88\u60f3\u3088\u308a\u65e9\u3044\u6bb5\u968e\u3067\u30c9\u30ed\u30c3\u30d7\u3057\u305f\u5834\u5408\u3068\u3001\u6ce2\u5f62\u5e8f\u76e4\u306e\u30b7\u30b0\u30ca\u30eb\u5f37\u5ea6\u304c\u9ad8\u304f\u30c8\u30c3\u30d7\u30d8\u30d3\u30fc\u306a\u72b6\u614b\u3068\u306a\u3063\u305f\u5834\u5408\u306b\u3064\u3044\u3066\u3001\u8003\u3048\u3089\u308c\u308b\u539f\u56e0\u3068\u5bfe\u51e6\u6cd5\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n

\u6ce2\u5f62\u306e\u9014\u4e2d\u3067\u30b7\u30b0\u30ca\u30eb\u304c\u30c9\u30ed\u30c3\u30d7\u3057\u3066\u3044\u308b<\/h2>\n

Raw Data\u306e\u30b7\u30b0\u30ca\u30eb\u304c\u4e88\u60f3\u3088\u308a\u65e9\u3044\u6bb5\u968e\u3067\u30c9\u30ed\u30c3\u30d7\u3057\u305f\u5834\u5408\uff08\u56f31\uff09\u3001\u89e3\u6790\u9818\u57df\u306e\u5869\u57fa\u914d\u5217\u304c\u539f\u56e0\u3067DNA\u306e\u4f38\u9577\u304c\u505c\u6b62\u3057\u305f\u53ef\u80fd\u6027\u304c\u8003\u3048\u3089\u308c\u307e\u3059\u3002\u3053\u306e\u5834\u5408\u3001\u71b1\u51e6\u7406\u3084\u5909\u6027\u5264\u306e\u6dfb\u52a0\u306a\u3069\u304c\u5bfe\u51e6\u6cd5\u3068\u306a\u308a\u307e\u3059\u3002\u4e0b\u8868\u306b\u3001\u4e88\u6e2c\u3055\u308c\u308b\u539f\u56e0\u3068\u5bfe\u51e6\u6cd5\u3092\u307e\u3068\u3081\u307e\u3059\u3002
\n

\"\u56f31.

\u56f31. \u30b7\u30b0\u30ca\u30eb\u5f37\u5ea6\u304c\u6025\u6fc0\u306b\u4f4e\u4e0b\u3057\u305f\u30c7\u30fc\u30bf\u4f8b \uff08Raw Data\uff09<\/p><\/div><\/p>\n\n\n\n\n\n\n
\u4e88\u6e2c\u3055\u308c\u308b\u539f\u56e0<\/b><\/td>\n\u5bfe\u51e6\u6cd5<\/b><\/td>\n<\/tr>\n
\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u306b\u304a\u3044\u3066\u3001\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u306e\u7279\u5b9a\u9818\u57df\u3067\u3001DNA \u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306b\u3088\u308bDNA\u306e\u4f38\u9577\u304c\u505c\u6b62\u3057\u305f<\/td>\n\n
    \n
  • \u30d8\u30a2\u30d4\u30f3\u306a\u3069\u306e\u4e8c\u6b21\u69cb\u9020\u304c\u539f\u56e0\u306e\u5834\u5408\u3001\u554f\u984c\u306e\u9818\u57df\u5468\u8fba\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u518d\u8a2d\u8a08\u3059\u308b\u3002\u6dfb\u52a0\u5264\uff085\uff5e10% DMSO\u3084\u30d9\u30bf\u30a4\u30f3\u3001\u30b0\u30ea\u30bb\u30ed\u30fc\u30eb\u306a\u3069\uff09\u306e\u4f7f\u7528\u3084\u3001\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u306e\u6700\u521d\u306e\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30b7\u30e7\u30f3\u30921\u5206\u304b\u308910\u5206\u306b\u5ef6\u9577\u3059\u308b\u3053\u3068\u3067\u3001\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u306e\u5909\u6027\u304c\u4fc3\u9032\u3055\u308c\u308b\u3068\u306e\u5831\u544a\u3082\u3042\u308b\u203b1<\/sup><\/li>\n
  • G\u30ea\u30c3\u30c1\u306a\u5869\u57fa\u914d\u5217\u306e\u5834\u5408\u3001Applied Biosystems\u2122 dGTP BigDye\u2122 Terminator Ready Reaction Cycle Sequencing Kit<\/a>\u306e\u4f7f\u7528\u3082\u9078\u629e\u80a2\u306e\u3072\u3068\u3064<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u306b\u4f7f\u7528\u3057\u305fApplied Biosystems\u2122 BigDye\u2122 Terminator Ready Reaction Mix\u306e\u91cf\u304c\u5c11\u306a\u3044<\/td>\nBigDye Terminator Ready Reaction Mix\u3092\u5e0c\u91c8\u3057\u3066\u4f7f\u7528\u3057\u3066\u3044\u308b\u5834\u5408\u3001\u6a19\u6e96\u30d7\u30ed\u30c8\u30b3\u30eb\u3078\u623b\u3059<\/td>\n<\/tr>\n
\u203b1 \u5f53\u793e\u3067\u306e\u30c6\u30b9\u30c8\u306f\u5b9f\u65bd\u3057\u3066\u304a\u308a\u307e\u305b\u3093\u3002<\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u30c8\u30c3\u30d7\u30d8\u30d3\u30fc\u30c7\u30fc\u30bf<\/h2>\n

Raw Data\u306e\u6700\u521d\u304b\u3089\u5f8c\u534a\u306b\u5411\u304b\u3044\u5f90\u3005\u306b\u30d4\u30fc\u30af\u9ad8\u3055\u304c\u4f4e\u4e0b\u3057\u3066\u3044\u308b\u5834\u5408\u3001\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u6db2\u306e\u7d44\u6210\u304c\u9069\u5207\u3067\u306a\u3044\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002
\n

\"\u56f32.

\u56f32. \u30b7\u30b0\u30ca\u30eb\u5f37\u5ea6\u304c\u5f90\u3005\u306b\u4f4e\u4e0b\u3057\u305f\u30c7\u30fc\u30bf\u4f8b \uff08Raw Data\uff09<\/p><\/div><\/p>\n\n\n\n\n\n\n\n
\u4e88\u6e2c\u3055\u308c\u308b\u539f\u56e0<\/b><\/td>\n\u3000<\/td>\n\u5bfe\u51e6\u6cd5<\/b><\/td>\n<\/tr>\n
\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u306e\u4f38\u9577\u6642\u9593\u304c\u77ed\u3044\u3001\u307e\u305f\u306f\u4f38\u9577\u6e29\u5ea6\u304c\u9ad8\u3044<\/td>\n\u3000<\/td>\n\u4f38\u9577\u6642\u9593\u3092\u5ef6\u3070\u3059\u304b\u3001\u4f38\u9577\u6e29\u5ea6\u3092\u4e0b\u3052\u308b<\/td>\n<\/tr>\n
\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\u53cd\u5fdc\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u5bfe\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u306e\u6bd4\u7387\u304c\u4e0d\u9069\u5207<\/td>\n\u3000<\/td>\n\u826f\u597d\u306a\u7d50\u679c\u304c\u5f97\u3089\u308c\u308b\u30d7\u30e9\u30a4\u30de\u30fc\u5bfe\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u306e\u6bd4\u7387\u3092\u691c\u8a0e\u3059\u308b<\/td>\n<\/tr>\n
\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u306b\u3001DNA \u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u6d3b\u6027\u3092\u963b\u5bb3\u3059\u308b\u3082\u306e\u304c\u30b3\u30f3\u30bf\u30df\u30cd\u30fc\u30b7\u30e7\u30f3\u3057\u3066\u3044\u308b<\/td>\n\u3000<\/td>\n\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u306e\u8abf\u88fd\u65b9\u6cd5\u3092\u78ba\u8a8d\u3057\u3001\u5225\u306e\u65b9\u6cd5\u3092\u8a66\u3059\u304b\u3001\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u3092\u7cbe\u88fd\u3059\u308b<\/td>\n<\/tr>\n
\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u306b\u4f7f\u7528\u3057\u305fBigDye Terminator Ready Reaction Mix\u306e\u91cf\u304c\u5c11\u306a\u3044<\/td>\n\u3000<\/td>\nBigDye Terminator Ready Reaction Mix\u3092\u5e0c\u91c8\u3057\u3066\u4f7f\u7528\u3057\u3066\u3044\u308b\u5834\u5408\u3001\u6a19\u6e96\u30d7\u30ed\u30c8\u30b3\u30eb\u3078\u623b\u3059<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Raw Data\u304c\u3001\u56f33\u306b\u793a\u3059\u3088\u3046\u306a\u6ce2\u5f62\u3068\u306a\u3063\u3066\u3044\u308b\u5834\u5408\u3001\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u3067\u3001\u77ed\u3044\u30b7\u30fc\u30b1\u30f3\u30b9\u7523\u7269\u304c\u512a\u5148\u7684\u306b\u751f\u6210\u3055\u308c\u3066\u3044\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u77ed\u3044\u30b7\u30fc\u30b1\u30f3\u30b9\u7523\u7269\u306f\u3001\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u3068\u3057\u305fPCR\u7523\u7269\u4e2d\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u30c0\u30a4\u30de\u30fc\u306b\u8d77\u56e0\u3059\u308b\u3053\u3068\u304c\u61f8\u5ff5\u3055\u308c\u308b\u305f\u3081\u3001PCR\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u518d\u8a2d\u8a08\u3057\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u30c0\u30a4\u30de\u30fc\u304c\u5f62\u6210\u3055\u308c\u308b\u30c7\u30b6\u30a4\u30f3\u3092\u9664\u5916\u3059\u308b\u3053\u3068\u304c\u5bfe\u51e6\u6cd5\u3068\u306a\u308a\u307e\u3059\u3002
\n

\"\u56f33.

\u56f33. \u77ed\u3044\u30b7\u30fc\u30b1\u30f3\u30b9\u7523\u7269\u304c\u512a\u5148\u7684\u306b\u751f\u6210\u3057\u305f\u5834\u5408\u306e\u30c7\u30fc\u30bf\u4f8b \uff08Raw Data\uff09<\/p><\/div><\/p>\n

\u307e\u3068\u3081<\/h2>\n

\u4eca\u56de\u306f\u3001\u30b7\u30b0\u30ca\u30eb\u304c\u4e88\u60f3\u3088\u308a\u65e9\u304f\u30c9\u30ed\u30c3\u30d7\u3057\u305f\u5834\u5408\u3068\u3001\u30c8\u30c3\u30d7\u30d8\u30d3\u30fc\u30c7\u30fc\u30bf\u3068\u306a\u3063\u305f\u5834\u5408\u306b\u3064\u3044\u3066\u3001\u8003\u3048\u3089\u308c\u308b\u539f\u56e0\u3068\u5bfe\u51e6\u6cd5\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3057\u305f\u3002\u30b7\u30fc\u30b1\u30f3\u30b9\u89e3\u6790\u306e\u7d50\u679c\u3001\u89e3\u8aad\u3067\u304d\u305f\u9577\u3055\u304c\u60f3\u5b9a\u3088\u308a\u77ed\u3044\u3068\u304d\u306f\u3001\u672c\u30d6\u30ed\u30b0\u306e\u5185\u5bb9\u3092\u53c2\u8003\u306b\u3057\u3066\u3044\u305f\u3060\u3051\u308c\u3070\u3068\u601d\u3044\u307e\u3059\u3002<\/p>\n

\u30ad\u30e3\u30d4\u30e9\u30ea\u30fc\u96fb\u6c17\u6cf3\u52d5\u306b\u3088\u308b\u30b7\u30fc\u30b1\u30f3\u30b9\u89e3\u6790\u306b\u3064\u3044\u3066\u3001\u3088\u308a\u8a73\u3057\u304f\u77e5\u308a\u305f\u3044\u65b9\u306f\u3001\u4e0b\u8a18\u3088\u308a\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u306e\u57fa\u672c\u539f\u5247\u3092\u5b66\u3079\u308b\u8cc7\u6599\u3092\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3044\u305f\u3060\u3051\u307e\u3059\u3002<\/p>\n

\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\u3000\u30cf\u30f3\u30c9\u30d6\u30c3\u30af<\/h3>\n

\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u306f\u3053\u3061\u3089<\/a><\/p>\n

\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\u30e9\u30fc\u30cb\u30f3\u30b0\u30bb\u30f3\u30bf\u30fc<\/a>\u304b\u3089\u3001\u30b7\u30fc\u30b1\u30f3\u30b9\u89e3\u6790\u306b\u95a2\u3059\u308b\u5404\u7a2e\u6280\u8853\u60c5\u5831\u306b\u30a2\u30af\u30bb\u30b9\u3044\u305f\u3060\u304f\u3053\u3068\u3082\u3067\u304d\u307e\u3059\u3002\u307e\u305f\u3001\u30b7\u30fc\u30b1\u30f3\u30b9\u89e3\u6790\u306e\u57fa\u790e\u30bb\u30df\u30ca\u30fc\u3084\u30cf\u30f3\u30ba\u30aa\u30f3\u30c8\u30ec\u30fc\u30cb\u30f3\u30b0\u3082\u3054\u3056\u3044\u307e\u3059\u306e\u3067\u3001\u305c\u3072\u3054\u6d3b\u7528\u304f\u3060\u3055\u3044\u3002
\n\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u89e3\u6790\u57fa\u790e\u30bb\u30df\u30ca\u30fc\uff081\uff09\u57fa\u672c\u539f\u7406<\/a>
\n\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u89e3\u6790\u57fa\u790e\u30bb\u30df\u30ca\u30fc\uff082\uff09\u30c7\u30fc\u30bf\u78ba\u8a8d\u3068\u30c8\u30e9\u30d6\u30eb\u30b7\u30e5\u30fc\u30c8<\/a>
\n\u5b8c\u5168\u30de\u30b9\u30bf\u30fc\uff01\u30cf\u30f3\u30ba\u30aa\u30f3\u30c8\u30ec\u30fc\u30cb\u30f3\u30b0\u30b3\u30fc\u30b9\u3000\u30ad\u30e3\u30d4\u30e9\u30ea\u30fc\u30b7\u30fc\u30b1\u30f3\u30b5\uff081\uff09 \u30b5\u30f3\u30d7\u30eb\u8abf\u88fd\u304b\u3089\u30c7\u30fc\u30bf\u78ba\u8a8d\u307e\u3067<\/a><\/p>\n

 <\/p>\n

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