{"id":19023,"date":"2025-02-05T17:35:39","date_gmt":"2025-02-05T08:35:39","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/?p=19023"},"modified":"2025-04-04T18:12:18","modified_gmt":"2025-04-04T09:12:18","slug":"qpcr-dna-workshop-gsd-ts-tr-25006","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-dna-workshop-gsd-ts-tr-25006\/","title":{"rendered":"\u3010\u3084\u3063\u3066\u307f\u305f\u3011\u71b1\u5909\u6027\u306e\u6e29\u5ea6\u3092\u4e0b\u3052\u305f\u5f71\u97ff\u3092\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3067\u78ba\u304b\u3081\u3066\u307f\u305f"},"content":{"rendered":"<p>PCR\u53cd\u5fdc\u306b\u304a\u3051\u308b\u71b1\u5909\u6027\uff08denature\uff09\u306f\u3001\u30a2\u30cb\u30fc\u30ea\u30f3\u30b0\uff08annealing\uff09\u3084\u4f38\u9577\u53cd\u5fdc\uff08extension\uff09\u3068\u4e26\u3076\u6b20\u304b\u3059\u3053\u3068\u306e\u3067\u304d\u306a\u3044\u30b9\u30c6\u30c3\u30d7\u306e1\u3064\u3067\u3059\uff08\u56f31\uff09\u3002\u71b1\u5909\u6027\u3067\u306f\u30012\u672c\u9396\u306e\u92f3\u578bDNA\u30921\u672c\u9396\u306b\u878d\u89e3\u3059\u308b\u3053\u3068\u3067\u3001\u6a19\u7684\u3068\u306a\u308b\u76f8\u88dc\u7684\u306aDNA\u9818\u57df\u306b\u30d7\u30e9\u30a4\u30de\u30fc\u304c\u7d50\u5408\u3059\u308b\u305f\u3081\u306e\u6e96\u5099\u3092\u3057\u3066\u3044\u307e\u3059\u3002\u3053\u306e\u71b1\u5909\u6027\u306e\u6e29\u5ea6\u306f94\uff5e98\u2103\u304c\u901a\u5e38\u3067\u3059\u304c\u3001\u6e29\u5ea6\u304c\u4f4e\u3059\u304e\u308b\u30682\u672c\u9396DNA\u304c1\u672c\u9396\u306b\u878d\u89e3\u305b\u305a\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u3082\u7d50\u5408\u3067\u304d\u306a\u304f\u306a\u308b\u306e\u3067PCR\u304c\u6210\u7acb\u3057\u306a\u304f\u306a\u3063\u3066\u3057\u307e\u3046\u3053\u3068\u304c\u8003\u3048\u3089\u308c\u307e\u3059\u3002\u3067\u306f\u3001\u3069\u306e\u304f\u3089\u3044\u307e\u3067\u6e29\u5ea6\u3092\u4e0b\u3052\u308b\u3068\u5f71\u97ff\u304c\u898b\u3089\u308c\u308b\u3088\u3046\u306b\u306a\u308b\u306e\u3067\u3057\u3087\u3046\u304b\u3002\u3084\u3063\u3066\u307f\u307e\u3057\u305f\u3002<\/p>\n<div id=\"attachment_19026\" style=\"width: 542px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-19026\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/02\/6558f305ff462605c985de99253a5889.jpg\" alt=\"\u56f31. PCR\u306e3\u3064\u306e\u4e3b\u8981\u30b9\u30c6\u30c3\u30d7\" width=\"532\" height=\"417\" class=\"size-full wp-image-19026\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/02\/6558f305ff462605c985de99253a5889.jpg 1520w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/02\/6558f305ff462605c985de99253a5889-300x235.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/02\/6558f305ff462605c985de99253a5889-1024x802.jpg 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/02\/6558f305ff462605c985de99253a5889-768x602.jpg 768w\" sizes=\"auto, (max-width: 532px) 100vw, 532px\" \/><p id=\"caption-attachment-19026\" class=\"wp-caption-text\">\u56f31. PCR\u306e3\u3064\u306e\u4e3b\u8981\u30b9\u30c6\u30c3\u30d7<\/p><\/div>\n<h2>\u6750\u6599\u3068\u65b9\u6cd5<\/h2>\n<p>\u92f3\u578b\u306eDNA\u306fHeLa cDNA\uff081 ng\/\u03bcL\uff09\u3092\u7528\u610f\u3057\u307e\u3057\u305f\u3002\u3053\u306ecDNA\u306f\u3001Invitrogen\u2122 <a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/12183020?CID=gsd_pcr_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_qpcr_dna_workshop_gsd_ts_tr_25006_Social_LAB\" target=\"_blank\">PureLink\u2122 RNA Mini Kit<\/a>\u3092\u4f7f\u7528\u3057\u3066HeLa\u7d30\u80de\u304b\u3089Total RNA\u3092\u62bd\u51fa\u3057\u305f\u5f8c\u3001Invitrogen\u2122 <a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/11756050?CID=gsd_pcr_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_qpcr_dna_workshop_gsd_ts_tr_25006_Social_LAB\" target=\"_blank\">SuperScript\u2122 IV VILO\u2122 Master Mix<\/a>\u3067\u9006\u8ee2\u5199\u53cd\u5fdc\u3092\u5b9f\u65bd\u3059\u308b\u3053\u3068\u3067\u5408\u6210\u3057\u307e\u3057\u305f\u3002\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u30de\u30b9\u30bf\u30fc\u30df\u30c3\u30af\u30b9\u306f\u3001Applied Biosystems\u2122 TaqMan\u2122 <a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/jp\/ja\/4444556?CID=gsd_pcr_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_qpcr_dna_workshop_gsd_ts_tr_25006_Social_LAB\" target=\"_blank\">Fast Advanced Master Mix<\/a> \u3092\u4f7f\u7528\u3057\u307e\u3057\u305f\u3002\u307e\u305f\u3001Applied Biosystems\u2122 TaqMan\u2122 <a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/pcr\/real-time-pcr\/real-time-pcr-assays.html?CID=gsd_pcr_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_qpcr_dna_workshop_gsd_ts_tr_25006_Social_LAB\" target=\"_blank\">Gene Expression Assay<\/a> (20x) GAPDH\u3092\u30bf\u30fc\u30b2\u30c3\u30c8\u3068\u3057\u307e\u3057\u305f\u3002\u92f3\u578b\u306eDNA\u306f2 \u03bcL\uff082 ng\/well\uff09\u4f7f\u7528\u3057\u3001\u88fd\u54c1\u306e\u53d6\u308a\u6271\u3044\u8aac\u660e\u66f8\u306b\u5f93\u304420 \u00b5L\u5bb9\u91cf\u306e\u53cd\u5fdc\u6db2\u3092\u8abf\u88fd\u3057\u307e\u3057\u305f\u3002\u5b9f\u9a13\u306b\u306fApplied Biosystems\u2122 <a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/pcr\/real-time-pcr\/real-time-pcr-instruments\/quantstudio-3-5-real-time-pcr-system\/quantstudio-5.html?CID=gsd_pcr_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_qpcr_dna_workshop_gsd_ts_tr_25006_Social_LAB\" target=\"_blank\">QuantStudio\u2122 5 Real-Time PCR System<\/a>\u3092\u4f7f\u7528\u3057\u3001\u4e0b\u8a18\u6761\u4ef6\u306e\u3088\u3046\u306b\u71b1\u5909\u6027\u6e29\u5ea6\u3092\u632f\u3063\u3066\u53cd\u5fdc\u3055\u305b\u3001C<sub>T<\/sub>\u5024\u306b\u3088\u3063\u3066\u71b1\u5909\u6027\u6e29\u5ea6\u306e\u5f71\u97ff\u3092\u8a55\u4fa1\u3057\u307e\u3057\u305f\uff08n = 8\u306e\u30c6\u30af\u30cb\u30ab\u30eb\u30ec\u30d7\u30ea\u30b1\u30fc\u30c8\uff09\u3002<\/p>\n<ol>\n<li>95\u2103\u300020 sec<\/li>\n<li>95\u2103 or 92\u2103 or 89\u2103 or 86\u2103 or 83\u2103 or 80\u2103\u30001 sec<\/li>\n<li>60\u2103\u300020 sec<\/li>\n<\/ol>\n<p>\u203b2\uff5e3\u309240 cycle\u53cd\u5fa9<\/p>\n<p>\u71b1\u5909\u6027\u306e\u6e29\u5ea6\u306f\u3001\u533a\u753b\u3054\u3068\u306b\u72ec\u7acb\u3057\u3066\u6e29\u5ea6\u5236\u5fa1\u3067\u304d\u308b\u6a5f\u80fd\uff08VeriFlex\uff09\u3092\u5229\u7528\u3059\u308b\u3053\u3068\u3067\u30011\u5ea6\u306e\u30e9\u30f3\u30676\u6e29\u5ea6\u6761\u4ef6\u3092\u540c\u6642\u306b\u6bd4\u8f03\u3057\u307e\u3057\u305f\uff08\u56f32\uff09\u3002<\/p>\n<div id=\"attachment_19028\" style=\"width: 580px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-19028\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/02\/99912360c677d369b317f5985bed2152.jpg\" alt=\"\u56f32. VeriFlex\u6a5f\u80fd\u306b\u3088\u308b\u71b1\u5909\u6027\u6e29\u5ea6\u6761\u4ef6\u306e\u8a2d\u5b9a\" width=\"570\" height=\"570\" class=\"size-full wp-image-19028\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/02\/99912360c677d369b317f5985bed2152.jpg 1728w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/02\/99912360c677d369b317f5985bed2152-300x300.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/02\/99912360c677d369b317f5985bed2152-1024x1024.jpg 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/02\/99912360c677d369b317f5985bed2152-150x150.jpg 150w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/02\/99912360c677d369b317f5985bed2152-768x768.jpg 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/02\/99912360c677d369b317f5985bed2152-1536x1536.jpg 1536w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/02\/99912360c677d369b317f5985bed2152-80x80.jpg 80w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/02\/99912360c677d369b317f5985bed2152-400x400.jpg 400w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/02\/99912360c677d369b317f5985bed2152-600x600.jpg 600w\" sizes=\"auto, (max-width: 570px) 100vw, 570px\" \/><p id=\"caption-attachment-19028\" class=\"wp-caption-text\">\u56f32. VeriFlex\u6a5f\u80fd\u306b\u3088\u308b\u71b1\u5909\u6027\u6e29\u5ea6\u6761\u4ef6\u306e\u8a2d\u5b9a<\/p><\/div>\n<h2>\u7d50\u679c\u3068\u8003\u5bdf<\/h2>\n<p>\u305d\u308c\u3067\u306f\u7d50\u679c\u3092\u78ba\u8a8d\u3057\u307e\u3057\u3087\u3046\u3002<br \/>\n\u307e\u305a\u306f\u3001\u71b1\u5909\u6027\u6e29\u5ea6\u309295\u2103\u304b\u308980\u2103\u307e\u3067\u632f\u3063\u305f\u5b9f\u9a13\u306e\u5897\u5e45\u66f2\u7dda\u3092\u78ba\u8a8d\u3057\u307e\u3059\uff08\u56f33\uff09\u3002<br \/>\n95\u2103\u300192\u2103\u300189\u2103\u306f\u5897\u5e45\u66f2\u7dda\u306b\u307b\u3068\u3093\u3069\u5dee\u304c\u7121\u3044\u3088\u3046\u306b\u898b\u3048\u307e\u3057\u305f\u3002\u71b1\u5909\u6027\u6e29\u5ea6\u309286\u2103\u307e\u3067\u4f4e\u4e0b\u3055\u305b\u308b\u3068\u660e\u3089\u304b\u306b\u5897\u5e45\u66f2\u7dda\u306e\u7acb\u3061\u4e0a\u304c\u308a\u304c\u9045\u304f\u306a\u308a\u300183\u2103\u307e\u3067\u4f4e\u4e0b\u3055\u305b\u308b\u3068\u3055\u3089\u306b\u7acb\u3061\u4e0a\u304c\u308a\u304c\u9045\u308c\u3070\u3089\u3064\u304d\u3082\u975e\u5e38\u306b\u5927\u304d\u304f\u306a\u308a\u307e\u3057\u305f\uff08\u56f33\u5de6\uff09\u3002\u71b1\u5909\u6027\u6e29\u5ea6\u306b\u3088\u308b\u5897\u5e45\u66f2\u7dda\u306e\u5dee\u3092\u8a73\u7d30\u306b\u78ba\u8a8d\u3057\u3066\u307f\u308b\u3068\u300195\u2103\u306892\u2103\u3067\u306f\u307b\u3068\u3093\u3069\u5dee\u304c\u3042\u308a\u307e\u305b\u3093\u3067\u3057\u305f\u304c\u300189\u2103\u3067\u306f\u5897\u5e45\u66f2\u7dda\u306e\u7acb\u3061\u4e0a\u304c\u308a\u304c\u3054\u304f\u308f\u305a\u304b\u306b\u9045\u308c\u305f\u3088\u3046\u306b\u898b\u3048\u307e\u3057\u305f\u3002\u307e\u305f\u300186\u2103\u3067\u306f\u5897\u5e45\u66f2\u7dda\u304c\u3070\u3089\u3064\u3044\u3066\u3044\u305f\u3053\u3068\u304c\u78ba\u8a8d\u3067\u304d\u307e\u3057\u305f\uff08\u56f33\u53f3\uff09\u3002\u3055\u3089\u306b\u300180\u2103\u307e\u3067\u71b1\u5909\u6027\u6e29\u5ea6\u3092\u4f4e\u4e0b\u3055\u305b\u308b\u3068\u5897\u5e45\u304c\u898b\u3089\u308c\u306a\u304f\u306a\u308a\u307e\u3057\u305f\u3002\u3053\u308c\u306f\u6e29\u5ea6\u304c\u4f4e\u3059\u304e\u30662\u672c\u9396DNA\u30921\u672c\u9396\u3078\u878d\u89e3\u3055\u305b\u308b\u3053\u3068\u304c\u3067\u304d\u305a\u3001PCR\u53cd\u5fdc\u304c\u5168\u304f\u9032\u307e\u306a\u304b\u3063\u305f\u3053\u3068\u3092\u793a\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<div id=\"attachment_19029\" style=\"width: 2570px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-19029\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/02\/697049510d184a62615c1c61a3b60ecb-scaled.jpg\" alt=\"\u56f33. \u71b1\u5909\u6027\u6e29\u5ea6\u306e\u9055\u3044\u306b\u3088\u308bPCR\u5897\u5e45\u3078\u306e\u5f71\u97ff\n\u5de6\uff1a\u901a\u5e38\u30b9\u30b1\u30fc\u30eb\u3001\u53f3\uff1a\u5de6\u30d1\u30cd\u30eb\u306e\u56db\u89d2\u90e8\u5206\u3092\u62e1\u5927\" width=\"2560\" height=\"1187\" class=\"size-full wp-image-19029\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/02\/697049510d184a62615c1c61a3b60ecb-scaled.jpg 2560w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/02\/697049510d184a62615c1c61a3b60ecb-300x139.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/02\/697049510d184a62615c1c61a3b60ecb-1024x475.jpg 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/02\/697049510d184a62615c1c61a3b60ecb-768x356.jpg 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/02\/697049510d184a62615c1c61a3b60ecb-1536x712.jpg 1536w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/02\/697049510d184a62615c1c61a3b60ecb-2048x949.jpg 2048w\" sizes=\"auto, (max-width: 2560px) 100vw, 2560px\" \/><p id=\"caption-attachment-19029\" class=\"wp-caption-text\">\u56f33. \u71b1\u5909\u6027\u6e29\u5ea6\u306e\u9055\u3044\u306b\u3088\u308bPCR\u5897\u5e45\u3078\u306e\u5f71\u97ff<br \/>\u5de6\uff1a\u901a\u5e38\u30b9\u30b1\u30fc\u30eb\u3001\u53f3\uff1a\u5de6\u30d1\u30cd\u30eb\u306e\u56db\u89d2\u90e8\u5206\u3092\u62e1\u5927<\/p><\/div>\n<p>\u6b21\u306b\u3001\u71b1\u5909\u6027\u6e29\u5ea6\u306e\u5f71\u97ff\u3092\u8a73\u7d30\u306b\u6bd4\u8f03\u3059\u308b\u305f\u3081\u3001C<sub>T<\/sub>\u5024\u3092\u6bd4\u8f03\u3057\u307e\u3057\u305f\uff08\u56f34\uff09\u3002\u5897\u5e45\u66f2\u7dda\u3067\u78ba\u8a8d\u3057\u305f\u901a\u308a\u300195\u2103\u306892\u2103\u3067\u306f\u307b\u3068\u3093\u3069\u5dee\u304c\u3042\u308a\u307e\u305b\u3093\u3067\u3057\u305f\u3002\u71b1\u5909\u6027\u6e29\u5ea6\u309289\u2103\u306b\u4f4e\u4e0b\u3055\u305b\u3066\u3082C<sub>T<\/sub>\u5024\u306f95\u2103\u308492\u2103\u3068\u307b\u307c\u540c\u3058\u3067\u3057\u305f\u304c\u3001\u308f\u305a\u304b\u306b\u3070\u3089\u3064\u304d\u304c\u5927\u304d\u304f\u306a\u3063\u3066\u3044\u307e\u3057\u305f\u3002\u3053\u306e\u3070\u3089\u3064\u304d\u304c\u539f\u56e0\u3067\u3001\u5897\u5e45\u66f2\u7dda\u306e\u7acb\u3061\u4e0a\u304c\u308a\u304c\u9045\u308c\u305f\u3088\u3046\u306b\u898b\u3048\u305f\uff08\u56f33\uff09\u3068\u8003\u3048\u3089\u308c\u307e\u3057\u305f\u3002\u3055\u3089\u306b\u71b1\u5909\u6027\u6e29\u5ea6\u309286\u2103\u306b\u4f4e\u4e0b\u3055\u305b\u308b\u3068\u300195\u2103\u3068\u6bd4\u8f03\u3057\u3066C<sub>T<\/sub>\u5024\u304c1.2\u307b\u3069\u5927\u304d\u304f\u306a\u308a\u3001\u71b1\u5909\u6027\u6e29\u5ea6\u306e\u5f71\u97ff\u304c\u73fe\u308c\u3066\u3044\u307e\u3057\u305f\u3002\u3055\u3089\u306b83\u2103\u306b\u307e\u3067\u4f4e\u4e0b\u3055\u305b\u308b\u3068\u300195\u2103\u3068\u6bd4\u8f03\u3057\u3066C<sub>T<\/sub>\u5024\u304c12.5\u307b\u3069\u5927\u304d\u304f\u306a\u308a\u3001C<sub>T<\/sub>\u5024\u306e\u3070\u3089\u3064\u304d\u3082\u9855\u8457\u306b\u5927\u304d\u304f\u306a\u3063\u3066\u3057\u307e\u3044\u307e\u3057\u305f\u3002<\/p>\n<div id=\"attachment_19030\" style=\"width: 573px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-19030\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/02\/1b994fc7857555f5fb4c81fc8996dca4.jpg\" alt=\"\u56f34. \u71b1\u5909\u6027\u6e29\u5ea6\u306e\u9055\u3044\u306b\u3088\u308bCT\u5024\u3078\u306e\u5f71\u97ff\" width=\"563\" height=\"564\" class=\"size-full wp-image-19030\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/02\/1b994fc7857555f5fb4c81fc8996dca4.jpg 1708w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/02\/1b994fc7857555f5fb4c81fc8996dca4-300x300.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/02\/1b994fc7857555f5fb4c81fc8996dca4-1024x1024.jpg 1024w, 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id=\"caption-attachment-19030\" class=\"wp-caption-text\">\u56f34. \u71b1\u5909\u6027\u6e29\u5ea6\u306e\u9055\u3044\u306b\u3088\u308bC<sub>T<\/sub>\u5024\u3078\u306e\u5f71\u97ff<\/p><\/div>\n<p>\u3053\u308c\u3089\u306e\u7d50\u679c\u304b\u3089\u3001\u4eca\u56de\u306e\u5b9f\u9a13\u6761\u4ef6\uff08\u92f3\u578b\u306e\u7a2e\u985e\u3001\u30de\u30b9\u30bf\u30fc\u30df\u30c3\u30af\u30b9\u306e\u7a2e\u985e\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u907a\u4f1d\u5b50\u306e\u7a2e\u985e\u306a\u3069\uff09\u3067\u306f\u3001\u6a19\u6e96\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\u306e\u71b1\u5909\u6027\u6e29\u5ea6\u3067\u3042\u308b95\u2103\u304b\u30893\u2103\u7a0b\u5ea6\u4f4e\u3055\u305b\u305f92\u2103\u3067\u3082PCR\u5897\u5e45\u306b\u306f\u307b\u3068\u3093\u3069\u5f71\u97ff\u304c\u306a\u3044\u3053\u3068\u304c\u5206\u304b\u308a\u307e\u3057\u305f\u300289\u2103\u307e\u3067\u4f4e\u4e0b\u3059\u308b\u3068\u3070\u3089\u3064\u304d\u304c\u5927\u304d\u304f\u306a\u3063\u305f\u306e\u3067\u71b1\u5909\u6027\u6e29\u5ea6\u306e\u5f71\u97ff\u304c\u3042\u3063\u305f\u3053\u3068\u304c\u793a\u5506\u3055\u308c\u307e\u3059\u3002\u3055\u3089\u306bGC\u30ea\u30c3\u30c1\u306a\u9818\u57df\u3092\u30bf\u30fc\u30b2\u30c3\u30c8\u306b\u3059\u308b\u5834\u5408\u3084\u3001cDNA\u3068\u6bd4\u8f03\u3057\u3066\u8907\u96d1\u6027\u304c\u9ad8\u304f\u30b5\u30a4\u30ba\u304c\u5927\u304d\u3044\u30b2\u30ce\u30e0DNA\u3092\u30b5\u30f3\u30d7\u30eb\u306b\u3059\u308b\u5834\u5408\u3067\u306f\u300192\u2103\u3067\u3082\u5f71\u97ff\u304c\u73fe\u308c\u308b\u53ef\u80fd\u6027\u3082\u8003\u3048\u3089\u308c\u307e\u3059\u3002\u307e\u305f\u3001\u4eca\u56de\u306f\u30d5\u30a9\u30ef\u30fc\u30c9\u30d7\u30e9\u30a4\u30de\u30fc\u3001\u30ea\u30d0\u30fc\u30b9\u30d7\u30e9\u30a4\u30de\u30fc\u306b\u52a0\u3048\u3001\u86cd\u5149\u6a19\u8b58\u3055\u308c\u305f\u30d7\u30ed\u30fc\u30d6\u3082\u7528\u3044\u308bTaqMan\u2122\u6cd5\u3067\u5b9f\u65bd\u3057\u307e\u3057\u305f\u304c\u3001\u30d5\u30a9\u30ef\u30fc\u30c9\u30d7\u30e9\u30a4\u30de\u30fc\u3068\u30ea\u30d0\u30fc\u30b9\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u307f\u4f7f\u7528\u3059\u308bSYBR\u2122 Green\u6cd5\u3067\u306f\u71b1\u5909\u6027\u6e29\u5ea6\u306e\u5f71\u97ff\u304c\u7570\u306a\u308b\u3053\u3068\u304c\u63a8\u5bdf\u3055\u308c\u307e\u3059\u3002<\/p>\n<h2>\u307e\u3068\u3081<\/h2>\n<p>\u4eca\u56de\u306e\u5b9f\u9a13\u3067\u306f\u3001\u71b1\u5909\u6027\u6e29\u5ea6\u304cPCR\u53cd\u5fdc\u306b\u4e0e\u3048\u308b\u5f71\u97ff\u3092\u8a73\u7d30\u306b\u691c\u8a0e\u3057\u307e\u3057\u305f\u3002\u6a19\u6e96\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\u306e95\u2103\u304b\u308992\u2103\u3078\u306e\u6e29\u5ea6\u4f4e\u4e0b\u3067\u306f\u307b\u3068\u3093\u3069\u5f71\u97ff\u304c\u898b\u3089\u308c\u307e\u305b\u3093\u3067\u3057\u305f\u304c\u300186\u2103\u4ee5\u4e0b\u3067\u306fPCR\u5897\u5e45\u306b\u660e\u3089\u304b\u306a\u5f71\u97ff\u304c\u3042\u308a\u300180\u2103\u307e\u3067\u4f4e\u4e0b\u3055\u305b\u308b\u3068DNA\u306e\u5897\u5e45\u304c\u5168\u304f\u898b\u3089\u308c\u307e\u305b\u3093\u3067\u3057\u305f\u3002\u3053\u308c\u3089\u306e\u7d50\u679c\u306f\u3001\u92f3\u578bDNA\u306e\u7a2e\u985e\u3084\u65b9\u6cd5\u306b\u3088\u3063\u3066\u7570\u306a\u308b\u53ef\u80fd\u6027\u304c\u3042\u308b\u305f\u3081\u3001\u8a66\u85ac\u306e\u53d6\u308a\u6271\u3044\u8aac\u660e\u66f8\u306b\u5f93\u3063\u305f\u3001\u9069\u5207\u306a\u5b9f\u9a13\u6761\u4ef6\u306e\u8a2d\u5b9a\u304c\u91cd\u8981\u3067\u3059\u3002<\/p>\n<h3>qPCR \u30d0\u30fc\u30c1\u30e3\u30eb\u6559\u5ba4 \u52d5\u753b\u30b3\u30f3\u30c6\u30f3\u30c4\u8996\u8074\u7533\u3057\u8fbc\u307f\u30d5\u30a9\u30fc\u30e0<\/h3>\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/qpcr-class-room-form.html?CID=gsd_pcr_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_qpcr_dna_workshop_gsd_ts_tr_25006_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">\u8a73\u7d30\u306f\u3053\u3061\u3089<\/a><\/p>\n<p>&nbsp;<\/p>\n<h3>\u5206\u5b50\u751f\u7269\u5b66\u6559\u5ba4PDF\u7248 \u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9<\/h3>\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/mol-bio-school.html?CID=gsd_pcr_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_qpcr_dna_workshop_gsd_ts_tr_25006_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u306f\u3053\u3061\u3089<\/a><\/p>\n<p><b>\u95a2\u9023\u8a18\u4e8b<\/b><br \/>\n<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/cloning\/cloning-learning-center\/invitrogen-school-of-molecular-biology\/pcr-education\/pcr-reagents-enzymes\/pcr-cycling-considerations.html?CID=gsd_pcr_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_qpcr_dna_workshop_gsd_ts_tr_25006_Social_LAB\" target=\"_blank\">PCR\u53cd\u5fdc\u6761\u4ef6\u306b\u304a\u3051\u308b6\u3064\u306e\u6ce8\u610f\u70b9<\/a><br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr_workshop_gsd_ts_1\/?CID=gsd_pcr_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_qpcr_dna_workshop_gsd_ts_tr_25006_Social_LAB\" target=\"_blank\">\u3010\u3084\u3063\u3066\u307f\u305f\u3011\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u30db\u30c3\u30c8\u30b9\u30bf\u30fc\u30c8\u9175\u7d20\u3092\u30db\u30c3\u30c8\u305b\u305a\u306b\u30b9\u30bf\u30fc\u30c8\u3057\u3066\u307f\u305f<\/a><br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr_workshop_gsd_ts_2\/?CID=gsd_pcr_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_qpcr_dna_workshop_gsd_ts_tr_25006_Social_LAB\" target=\"_blank\">\u3010\u3084\u3063\u3066\u307f\u305f\u3011\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u30db\u30c3\u30c8\u30b9\u30bf\u30fc\u30c8\u9175\u7d20\u3092\u30a6\u30a9\u30fc\u30e0\u30b9\u30bf\u30fc\u30c8\u3057\u3066\u307f\u305f<\/a><\/p>\n<p>&nbsp;<\/p>\n<p>\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u7528\u306b\u306f\u4f7f\u7528\u3044\u305f\u3060\u3051\u307e\u305b\u3093\u3002<\/p>\n<p>TaqMan is a trademark of Roche Molecular Systems, Inc., used under permission and license. 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