{"id":19938,"date":"2025-03-12T14:00:50","date_gmt":"2025-03-12T05:00:50","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/?p=19938"},"modified":"2025-03-12T16:55:31","modified_gmt":"2025-03-12T07:55:31","slug":"cell-culture-cell-count-bid-ts-mbr-25012","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/cell-culture-cell-count-bid-ts-mbr-25012\/","title":{"rendered":"\u3010\u3084\u3063\u3066\u307f\u305f\u3011\u201d\u3084\u3055\u3057\u3044\u201d\u7d30\u80de\u89e3\u96e2\u8a66\u85ac\u300cTrypLE\u300d\u3067\u7d30\u80de\u3092\u304d\u3073\u3057\u304f\u51e6\u7406\u3057\u3066\u307f\u305f"},"content":{"rendered":"<p>Trypsin-EDTA\u306f\u63a5\u7740\u7d30\u80de\u306e\u89e3\u96e2\u64cd\u4f5c\u306b\u6c4e\u7528\u3055\u308c\u3066\u3044\u308b\u8a66\u85ac\u3067\u3059\u3002\u30bf\u30f3\u30d1\u30af\u8cea\u5206\u89e3\u9175\u7d20\u3067\u3042\u308bTrypsin\u304c\u7d30\u80de\u63a5\u7740\u5206\u5b50\u3092\u6d88\u5316\u3057\u3001EDTA\u304c\u7d30\u80de\u63a5\u7740\u306b\u5fc5\u8981\u306aCa2<sup>+<\/sup>\u3068Mg2<sup>+<\/sup>\u3092\u30ad\u30ec\u30fc\u30c8\u3057\u3066\u7d30\u80de\u306e\u89e3\u96e2\u3092\u4fc3\u9032\u3057\u307e\u3059\u3002\u4e00\u822c\u306bTrypsin\u306f\u30d6\u30bf\u306a\u3069\u306e\u81b5\u81d3\u304b\u3089\u7cbe\u88fd\u3057\u305f\u3082\u306e\u3092\u539f\u6750\u6599\u3068\u3059\u308b\u305f\u3081\u3001\u81b5\u81d3\u306b\u5b58\u5728\u3059\u308bTrypsin\u4ee5\u5916\u306e\u6d88\u5316\u9175\u7d20\u306e\u6b8b\u5b58\u304c\u907f\u3051\u3089\u308c\u305a\u3001\u3053\u308c\u304c\u7d30\u80de\u6bd2\u6027\u306b\u3064\u306a\u304c\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u3053\u306e\u554f\u984c\u306e\u89e3\u6c7a\u7b56\u3068\u3057\u3066\u3001\u5f53\u793e\u306f\u72ec\u81ea\u306e\u7d30\u80de\u89e3\u96e2\u8a66\u85ac\u300c<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/cell-culture\/mammalian-cell-culture\/reagents\/trypsin\/tryple-express.html?CID=bid_clb_r04_jp_cp0000_pjt0000_bid00000_0so_blg_cell_culture_cell_count_bid_ts_mbr_25012_Social_LAB\" target=\"_blank\">TrypLE\u8a66\u85ac\u30b7\u30ea\u30fc\u30ba<\/a>\u300d\u3092\u63d0\u4f9b\u3057\u3066\u3044\u307e\u3059\u3002\u3053\u306e\u8a66\u85ac\u306f\u3001Trypsin\u3068\u540c\u3058\u304f\u30ea\u30b8\u30f3\u3068\u30a2\u30eb\u30ae\u30cb\u30f3\u6b8b\u57fa\u306eC\u672b\u5074\u3092\u5207\u65ad\u3059\u308b\u9175\u7d20\u3068EDTA\u3092\u542b\u3093\u3067\u304a\u308a\u3001Trypsin-EDTA\u306e\u4ee3\u308f\u308a\u306b\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u7d30\u83cc\u306b\u7523\u751f\u3055\u305b\u305f\u7d44\u307f\u63db\u3048\u9175\u7d20\u3092\u539f\u6599\u3068\u3057\u3066\u3044\u308b\u305f\u3081\u3001Trypsin-EDTA\u3067\u61f8\u5ff5\u3055\u308c\u308b\u593e\u96d1\u9175\u7d20\u304c\u542b\u307e\u308c\u3066\u3044\u306a\u3044\u3053\u3068\u304b\u3089\u3001Trypsin-EDTA\u3068\u6bd4\u3079\u3066\u7d30\u80de\u306b\u201d\u3084\u3055\u3057\u3044\u201d\u9175\u7d20\u3060\u3068\u8a00\u308f\u308c\u3066\u3044\u307e\u3059\u3002\u4ee5\u524d\u306e\u8a18\u4e8b\u3067\u3001\u904e\u5270\u306aTrypsin\u51e6\u7406\u304c\u7d30\u80de\u306e\u751f\u5b58\u7387\u3084\u751f\u7d30\u80de\u6570\u306b\u53ca\u307c\u3059\u5f71\u97ff\u3092\u78ba\u8a8d\u3057\u307e\u3057\u305f\uff08\u95a2\u9023\u8a18\u4e8b\uff1a<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/cell-culture-cell-count-workshop-bid-ts-mbr-24080\/?CID=bid_clb_r04_jp_cp0000_pjt0000_bid00000_0so_blg_cell_culture_cell_count_bid_ts_mbr_25012_Social_LAB\" target=\"_blank\">\u3010\u3084\u3063\u3066\u307f\u305f\u3011\u7d30\u80de\u3092Trypsin\u3067\u51e6\u7406\u3057\u3059\u304e\u3066\u307f\u305f<\/a>\uff09\u3002\u305d\u3053\u3067\u4eca\u56de\u306f\u7d30\u80de\u3092TrypLE\u8a66\u85ac\u3067\u904e\u5270\u306b\u51e6\u7406\u3057\u3066\u3001\u751f\u5b58\u7387\u3084\u7d30\u80de\u6570\u3078\u306e\u5f71\u97ff\u3092\u691c\u8a3c\u3057\u307e\u3057\u305f\u3002<\/p>\n<h2>\u5b9f\u9a13\u65b9\u6cd5<\/h2>\n<p>\u25a0\u7d30\u80de<br \/>\nHeLa\u7d30\u80de\u306e\u61f8\u6fc1\u6db2<\/p>\n<p>\u25a0\u624b\u9806<\/p>\n<ol>\n<li>\u7d30\u80de\u61f8\u6fc1\u6db2\u3092\u9060\u5fc3\u5206\u96e2<\/li>\n<li>\u4e0a\u6f84\u307f\u3092\u30a2\u30b9\u30d4\u30ec\u30fc\u30c8\u3057\u3066Gibco\u2122 DPBS\uff08\u88fd\u54c1\u756a\u53f7 <a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/14190144?CID=bid_clb_r04_jp_cp0000_pjt0000_bid00000_0so_blg_cell_culture_cell_count_bid_ts_mbr_25012_Social_LAB\" target=\"_blank\">14190144<\/a>\uff09\u3067\u518d\u61f8\u6fc1\n<li>\u7d30\u80de\u61f8\u6fc1\u6db2\u3092\u9060\u5fc3\u5206\u96e2<\/li>\n<li>Gibco\u2122 CTS\u2122 TrypLE\u2122 Select Enzyme\uff08\u88fd\u54c1\u756a\u53f7 <a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/A1285901?CID=bid_clb_r04_jp_cp0000_pjt0000_bid00000_0so_blg_cell_culture_cell_count_bid_ts_mbr_25012_Social_LAB\" target=\"_blank\">A1285901<\/a>\uff09\u3067\u518d\u61f8\u6fc1<\/li>\n<li>5\u672c\u306e\u30c1\u30e5\u30fc\u30d6\u306b\u5c0f\u5206\u3051<\/li>\n<li>37\u2103\u306e\u6e29\u6d74\u306710\u300120\u300130\u300160\u5206\u9593\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30c8<\/li>\n<li>Gibco\u2122 Trypan Blue\u6eb6\u6db2\uff08\u88fd\u54c1\u756a\u53f7 <a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/15250061?CID=bid_clb_r04_jp_cp0000_pjt0000_bid00000_0so_blg_cell_culture_cell_count_bid_ts_mbr_25012_Social_LAB\" target=\"_blank\">15250061<\/a>\uff09\u3068\u7d30\u80de\u61f8\u6fc1\u6db2\u30921:1\u3067\u6df7\u5408\u3057\u3066Invitrogen\u2122 Countess\u2122 Chamber Slide\uff08\u88fd\u54c1\u756a\u53f7 <a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/C10283?CID=bid_clb_r04_jp_cp0000_pjt0000_bid00000_0so_blg_cell_culture_cell_count_bid_ts_mbr_25012_Social_LAB\" target=\"_blank\">C10283<\/a>\uff09\u306b\u6ce8\u5165<\/li>\n<li>Invitrogen\u2122 <a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/cell-analysis\/cell-analysis-instruments\/automated-cell-counters\/models\/countess-3-fl.html?CID=bid_clb_r04_jp_cp0000_pjt0000_bid00000_0so_blg_cell_culture_cell_count_bid_ts_mbr_25012_Social_LAB\" target=\"_blank\">Countess 3 FL\u81ea\u52d5\u30bb\u30eb\u30ab\u30a6\u30f3\u30bf\u30fc<\/a>\u306e\u660e\u8996\u91ce\u30e2\u30fc\u30c9\u3067\u81ea\u52d5\u30bb\u30eb\u30ab\u30a6\u30f3\u30c8<\/li>\n<p>\u203b\u7d30\u80de\u306e\u7834\u7247\u306f\u30d1\u30e9\u30e1\u30fc\u30bf\u30fc\u8abf\u7bc0\u6a5f\u80fd\u3067\u9664\u5916\n<\/li>\n<\/ol>\n<h2>\u904e\u5270\u306aTrypLE\u51e6\u7406\u304c\u7d30\u80de\u306e\u751f\u5b58\u7387\u306b\u53ca\u307c\u3059\u5f71\u97ff<\/h2>\n<p>\u3055\u3063\u305d\u304f\u7d50\u679c\u3092\u898b\u3066\u3044\u304d\u307e\u3057\u3087\u3046\u3002HeLa\u7d30\u80de\u3092TrypLE\u8a66\u85ac\u3067\u61f8\u6fc1\u3057\u305f\u6eb6\u6db2\u309237\u2103\u30670\u300110\u300120\u300130\u300160\u5206\u9593\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30c8\u3057\u305f\u3068\u304d\u306e\u751f\u5b58\u7387\u3092\u56f3\uff11\u306b\u307e\u3068\u3081\u307e\u3057\u305f\u3002<\/p>\n<div id=\"attachment_19949\" style=\"width: 410px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-19949\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/03\/19950885a096aeb0999a96f321a04f87-300x276.jpg\" alt=\"\u56f31. \u904e\u5270\u306aTrypLE\u51e6\u7406\u306e\u751f\u5b58\u7387\u3078\u306e\u5f71\u97ff\" width=\"400\" height=\"368\" class=\"size-medium wp-image-19949\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/03\/19950885a096aeb0999a96f321a04f87-300x276.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/03\/19950885a096aeb0999a96f321a04f87-768x706.jpg 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/03\/19950885a096aeb0999a96f321a04f87.jpg 996w\" sizes=\"auto, (max-width: 400px) 100vw, 400px\" \/><p id=\"caption-attachment-19949\" class=\"wp-caption-text\">\u56f31. \u904e\u5270\u306aTrypLE\u51e6\u7406\u306e\u751f\u5b58\u7387\u3078\u306e\u5f71\u97ff<\/p><\/div>\n<p>Trypsin\u3067\u904e\u5270\u306b\u51e6\u7406\u3057\u305f\u691c\u8a3c\u3067\u306f\u51e6\u740630\u5206\u5f8c\u306b\u306f\u751f\u5b58\u7387\u304c\u5927\u304d\u304f\u4f4e\u4e0b\u3057\u307e\u3057\u305f\uff08\u95a2\u9023\u8a18\u4e8b\uff1a<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/cell-culture-cell-count-workshop-bid-ts-mbr-24080\/?CID=bid_clb_r04_jp_cp0000_pjt0000_bid00000_0so_blg_cell_culture_cell_count_bid_ts_mbr_25012_Social_LAB\" target=\"_blank\">\u3010\u3084\u3063\u3066\u307f\u305f\u3011\u7d30\u80de\u3092Trypsin\u3067\u51e6\u7406\u3057\u3059\u304e\u3066\u307f\u305f<\/a>\uff09\u3002\u4e00\u65b9\u4eca\u56de\u306eTrypLE\u3067\u306f\u300130\u5206\u5f8c\u306860\u5206\u5f8c\u306e\u751f\u5b58\u7387\u304c\u308f\u305a\u304b\u306b\u4f4e\u4e0b\u50be\u5411\u3067\u306f\u3042\u308b\u3082\u306e\u306e\u300190%\u4ee5\u4e0a\u306e\u751f\u5b58\u7387\u3092\u7dad\u6301\u3057\u3066\u3044\u307e\u3057\u305f\u3002\u3053\u308c\u306f\u3001TrypLE\u304cTrypsin\u3088\u308a\u3082\u201c\u3084\u3055\u3057\u3044\u201d\u3053\u3068\u306e\u3042\u3089\u308f\u308c\u306a\u306e\u3067\u3057\u3087\u3046\u304b\uff1f\u305d\u308c\u3092\u78ba\u304b\u3081\u308b\u305f\u3081\u306b\u3001\u751f\u7d30\u80de\u306e\u6fc3\u5ea6\u306e\u7d99\u6642\u5909\u5316\u3092\u56f32\u306b\u307e\u3068\u3081\u307e\u3057\u305f\u3002<\/p>\n<div id=\"attachment_19954\" style=\"width: 410px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-19954\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/03\/e44fe122baf9d37995caf9ac11e4a570-300x284.jpg\" alt=\"\u56f32. \u904e\u5270\u306aTrypLE\u51e6\u7406\u306e\u751f\u7d30\u80de\u6fc3\u5ea6\u3078\u306e\u5f71\u97ff\" width=\"400\" height=\"368\" class=\"size-medium wp-image-19954\" \/><p id=\"caption-attachment-19954\" class=\"wp-caption-text\">\u56f32. \u904e\u5270\u306aTrypLE\u51e6\u7406\u306e\u751f\u7d30\u80de\u6fc3\u5ea6\u3078\u306e\u5f71\u97ff<\/p><\/div>\n<p>\u751f\u7d30\u80de\u306e\u6fc3\u5ea6\u306f\u6700\u521d\u306e10\u5206\u9593\u306e\u51e6\u7406\u30673\u5272\u4ee5\u4e0a\u4f4e\u4e0b\u3057\u3001\u305d\u306e\u5f8c\u3082\u5f90\u3005\u306b\u4f4e\u4e0b\u3059\u308b\u50be\u5411\u304c\u898b\u3089\u308c\u307e\u3057\u305f\u3002\u3064\u307e\u308a\u3001\u7d30\u80de\u306b\u201d\u3084\u3055\u3057\u3044\u201d\u3068\u3055\u308c\u308bTrypLE\u3067\u3042\u3063\u3066\u3082\u3001\u904e\u5270\u306a\u51e6\u7406\u306f\u7d30\u80de\u306b\u60aa\u5f71\u97ff\u304c\u3042\u308b\u3053\u3068\u304c\u78ba\u304b\u3081\u3089\u308c\u307e\u3057\u305f\u3002\u751f\u5b58\u7387\u3092\u6307\u6a19\u306b\u3059\u308b\u3068\u5f71\u97ff\u304c\u8efd\u5fae\u306b\u898b\u3048\u308b\u306b\u3082\u304b\u304b\u308f\u3089\u305a\uff08\u56f31\uff09\u3001\u751f\u7d30\u80de\u306e\u6fc3\u5ea6\u3068\u3057\u3066\u306f\u9855\u8457\u306b\u5f71\u97ff\u3092\u53d7\u3051\u3066\u3044\u308b\uff08\u56f32\uff09\u306e\u306f\u8208\u5473\u6df1\u3044\u3067\u3059\u3002\u3053\u308c\u306fCountess\u306b\u3088\u308b\u81ea\u52d5\u30bb\u30eb\u30ab\u30a6\u30f3\u30c8\u3068Trypan Blue\u306b\u3088\u308b\u751f\u6b7b\u5224\u5b9a\u306e\u539f\u7406\u304c\u95a2\u4fc2\u3057\u3066\u3044\u308b\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002\u904e\u5270\u306aTrypLE\u51e6\u7406\u306b\u3088\u3063\u3066\u640d\u50b7\u3057\u305f\u7d30\u80de\u304c\u3059\u307f\u3084\u304b\u306b\u7834\u7247\u306b\u306a\u3063\u3066\u3044\u308b\u3068\u4eee\u5b9a\u3059\u308b\u3068\u3001Countess\u81ea\u52d5\u30bb\u30eb\u30ab\u30a6\u30f3\u30bf\u30fc\u306b\u3088\u308b\u30ab\u30a6\u30f3\u30c8\u306e\u5bfe\u8c61\u5916\u3068\u306a\u3063\u3066\u6b7b\u7d30\u80de\u7387\u3092\u4e0a\u6607\u3055\u305b\u305a\u306b\u751f\u7d30\u80de\u306e\u6fc3\u5ea6\u304c\u4f4e\u4e0b\u3059\u308b\u3053\u3068\u306b\u306a\u308a\u307e\u3059\u3002\u4eca\u56de\u306e\u7d50\u679c\u306f\u3001\u4f55\u3089\u304b\u306e\u51e6\u7406\u304c\u7d30\u80de\u306e\u751f\u6b7b\u306b\u4e0e\u3048\u308b\u5f71\u97ff\u3092\u5224\u65ad\u3059\u308b\u969b\u306b\u306f\u3001Trypan Blue\u306e\u751f\u6b7b\u5224\u5b9a\u3060\u3051\u3067\u306a\u304f\u7d30\u80de\u306e\u6fc3\u5ea6\uff08\u7d30\u80de\u6570\uff09\u3082\u78ba\u8a8d\u3057\u305f\u307b\u3046\u304c\u3088\u3044\u3053\u3068\u3092\u793a\u5506\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<h2>\u904e\u5270\u306aTrypLE\u51e6\u7406\u306e\u7d30\u80de\u306e\u5897\u6b96\u3078\u306e\u5f71\u97ff<\/h2>\n<p>\u6700\u5f8c\u306b\u3001\u5404\u51e6\u7406\u6761\u4ef6\u304c\u7d30\u80de\u306e\u305d\u306e\u5f8c\u306e\u5897\u6b96\u306b\u5f71\u97ff\u3092\u4e0e\u3048\u308b\u304b\u3069\u3046\u304b\u3092\u78ba\u8a8d\u3059\u308b\u305f\u3081\u3001\u7d30\u80de\u57f9\u990a\u30d7\u30ec\u30fc\u30c8\u306b\u64ad\u7a2e\u3057\u3066\u57f9\u990a3\u65e5\u9593\u57f9\u990a\u3057\u307e\u3057\u305f\u3002\u305d\u308c\u305e\u308c\u306e\u751f\u7d30\u80de\u306e\u6570\u3092\u6e2c\u5b9a\u3057\u305f\u7d50\u679c\u3092\u56f33\u306b\u307e\u3068\u3081\u307e\u3057\u305f\u3002<\/p>\n<div id=\"attachment_19957\" style=\"width: 410px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-19957\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/03\/c747ead17a89d82d3c02a84b85346af5-298x300.jpg\" alt=\"\u56f33\uff0e\u904e\u5270\u306aTrypLE\u51e6\u7406\u306b\u3088\u308b\u7d30\u80de\u5897\u6b96\u3078\u306e\u5f71\u97ff\" width=\"400\" height=\"368\" class=\"size-medium wp-image-19957\" \/><p id=\"caption-attachment-19957\" class=\"wp-caption-text\">\u56f33\uff0e\u904e\u5270\u306aTrypLE\u51e6\u7406\u306b\u3088\u308b\u7d30\u80de\u5897\u6b96\u3078\u306e\u5f71\u97ff<\/p><\/div>\n<p>TrypLE\u51e6\u7406\u306e\u6642\u9593\u304c\u9577\u304f\u306a\u308b\u306b\u3064\u308c\u3066\u751f\u7d30\u80de\u6570\u304c\u6e1b\u3063\u3066\u3044\u304f\u50be\u5411\u304c\u307f\u3089\u308c\u307e\u3057\u305f\u304c\u3001\u51e6\u74060\u5206\u304b\u3089\u51e6\u740630\u5206\u306e\u6e1b\u5c11\u7387\u3068\u6bd4\u8f03\u3057\u3066\u3001\u51e6\u740630\u5206\u304b\u3089\u51e6\u740660\u5206\u306e\u65b9\u304c\u5927\u304d\u304f\u6e1b\u5c11\u3057\u307e\u3057\u305f\u3002\u56f32\u306e\u7d50\u679c\u304b\u3089\u3001\u51e6\u740630\u5206\u306860\u5206\u306e\u30b5\u30f3\u30d7\u30eb\u306e\u30d7\u30ec\u30fc\u30c8\u64ad\u7a2e\u6642\u306e\u751f\u7d30\u80de\u6570\u306f\u307b\u307c\u540c\u7b49\u3060\u3063\u305f\u3053\u3068\u304b\u3089\u3001Trypan Blue\u306b\u3088\u308b\u751f\u6b7b\u5224\u5b9a\u3067\u306f\u691c\u51fa\u3067\u304d\u306a\u3044\u30c0\u30e1\u30fc\u30b8\u3092\u51e6\u740660\u5206\u306e\u7d30\u80de\u306f30\u5206\u306e\u7d30\u80de\u3088\u308a\u3082\u5f37\u304f\u53d7\u3051\u3066\u3044\u305f\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002\u7d30\u80de\u306e\u8868\u9762\u306b\u306f\u7d30\u80de\u63a5\u7740\u306b\u95a2\u308f\u308b\u5206\u5b50\u4ee5\u5916\u306b\u3001\u5916\u90e8\u304b\u3089\u306e\u5897\u6b96\u56e0\u5b50\u3092\u53d7\u3051\u53d6\u308b\u53d7\u5bb9\u4f53\u5206\u5b50\u306a\u3069\u3001\u3055\u307e\u3056\u307e\u306a\u6a5f\u80fd\u306e\u5206\u5b50\u304c\u5b58\u5728\u3057\u3066\u3044\u307e\u3059\u3002\u3053\u306e\u7d50\u679c\u306f\u3001\u6d88\u5316\u3055\u308c\u305f\u7d30\u80de\u8868\u9762\u5206\u5b50\u306e\u6570\u306e\u5dee\u306e\u53cd\u6620\u306a\u306e\u304b\u3082\u3057\u308c\u307e\u305b\u3093\u3002\u57f9\u990a3\u65e5\u5f8c\u306e\u7d30\u80de\u306e\u72b6\u614b\u3092\u89b3\u5bdf\u3059\u308b\u3068\u3001\u51e6\u740660\u5206\u306e\u7d30\u80de\u3067\u306f\u30d7\u30ec\u30fc\u30c8\u306e\u5e95\u9762\u306b\u63a5\u7740\u3057\u304d\u308c\u3066\u3044\u306a\u3044\u7d30\u80de\u304c\u6563\u898b\u3055\u308c\u3001\u51e6\u74060\u5206\u308430\u5206\u3068\u6bd4\u3079\u3066\u3082\u30c0\u30e1\u30fc\u30b8\u304c\u6b8b\u3063\u3066\u3044\u308b\u3088\u3046\u306b\u898b\u3048\u307e\u3057\u305f\u3002<\/p>\n<div id=\"attachment_19959\" style=\"width: 510px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-19959\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/03\/db4d9ffec556f6b07e9b55d8b430e611-300x149.png\" alt=\"\" width=\"500\" height=\"249\" class=\"size-medium wp-image-19959\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/03\/db4d9ffec556f6b07e9b55d8b430e611-300x149.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/03\/db4d9ffec556f6b07e9b55d8b430e611-1024x507.png 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/03\/db4d9ffec556f6b07e9b55d8b430e611.png 1610w\" sizes=\"auto, (max-width: 500px) 100vw, 500px\" \/><p id=\"caption-attachment-19959\" class=\"wp-caption-text\">\u56f34. \u904e\u5270\u306aTrypLE\u51e6\u7406\u306b\u3088\u308b\u7d30\u80de\u306e\u5897\u6b96\u3084\u63a5\u7740\u3078\u306e\u5f71\u97ff<br \/><\/p><\/div>\n<h2>\u307e\u3068\u3081<\/h2>\n<p>\u4eca\u56de\u306f\u3001Trypsin-EDTA\u306e\u304b\u308f\u308a\u3068\u306a\u308a\u3046\u308b\u7d30\u80de\u89e3\u96e2\u8a66\u85ac\u300cTrypLE Select Enzyme\u300d\u3067\u7d30\u80de\u3092\u904e\u5270\u306b\u51e6\u7406\u3057\u3001\u7d30\u80de\u306e\u751f\u5b58\u3084\u305d\u306e\u5f8c\u306e\u57f9\u990a\u3078\u306e\u5f71\u97ff\u3092\u691c\u8a3c\u3057\u307e\u3057\u305f\u3002\u593e\u96d1\u9175\u7d20\u304c\u7121\u3044\u305f\u3081Trypsin\u3088\u308a\u3082\u201d\u3084\u3055\u3057\u3044\u201d\u3068\u3055\u308c\u308bTrypLE Select Enzyme\u3067\u3059\u304c\u3001\u904e\u5270\u306a\u51e6\u7406\u306f\u3084\u306f\u308a\u7d30\u80de\u306b\u30c0\u30e1\u30fc\u30b8\u3092\u4e0e\u3048\u308b\u3053\u3068\u304c\u78ba\u8a8d\u3067\u304d\u307e\u3057\u305f\u3002\u3054\u4f7f\u7528\u306e\u969b\u306b\u306f\u3001Trypsin-EDTA\u306e\u5834\u5408\u3068\u540c\u69d8\u306b\u3001\u7d30\u80de\u306e\u89e3\u96e2\u306b\u5fc5\u8981\u306a\u6700\u5c0f\u9650\u306e\u51e6\u7406\u306e\u51e6\u7406\u3068\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n<p>TrypLE Select Enzyme\u306f\u7d30\u80de\u3078\u306e\u4f5c\u7528\u4ee5\u5916\u306b\u3082\u3001\u975e\u5e38\u306b\u5b89\u5b9a\u6027\u304c\u9ad8\u3044\u3053\u3068\u304b\u3089\u5ba4\u6e29\u4fdd\u5b58\u304c\u53ef\u80fd\u306a\u3068\u3053\u308d\u3082Trypsin-EDTA\u3088\u308a\u3082\u4fbf\u5229\u306a\u30dd\u30a4\u30f3\u30c8\u3067\u3059\u3002\u3082\u3061\u308d\u3093\u51b7\u8535\u3001\u51b7\u51cd\u4fdd\u5b58\u3082\u53ef\u80fd\u3067\u3059\u3002\u4eca\u56de\u306e\u5b9f\u9a13\u306f\u300cGibco\u2122 CTS\u2122 TrypLE\u2122 Select Enzyme\u300d\u3092\u4f7f\u7528\u3057\u307e\u3057\u305f\u304c\u3001\u3053\u306e\u307b\u304b\u306b\u3082\u300cGibco\u2122 TrypLE\u2122 Express Enzyme\u300d\u3001\u300cGibco\u2122 TrypLE\u2122 Select\u300d\u3092\u63d0\u4f9b\u3057\u3066\u3044\u307e\u3059\u3002\u8a66\u85ac\u306b\u542b\u307e\u308c\u308b\u9175\u7d20\u306e\u6027\u80fd\u306f\u540c\u7b49\u3067\u3059\u306e\u3067\u3001\u4eca\u56de\u306e\u691c\u8a3c\u7d50\u679c\u306f\u3053\u308c\u3089\u306e\u8a66\u85ac\u3059\u3079\u3066\u306b\u5f53\u3066\u306f\u307e\u308a\u307e\u3059\u3002TrypLE\u30b7\u30ea\u30fc\u30ba\u306e\u7279\u9577\u3068\u30e9\u30a4\u30f3\u30ca\u30c3\u30d7\u306b\u3064\u3044\u3066\u306f\u3001\u95a2\u9023\u8a18\u4e8b\u300c<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/cell-culture-cell-separation-bid-ts-mbr-24001\/?CID=bid_clb_r04_jp_cp0000_pjt0000_bid00000_0so_blg_cell_culture_cell_count_bid_ts_mbr_25012_Social_LAB\" target=\"_blank\">\u30b7\u30f3\u30b0\u30eb\u30bb\u30eb\u89e3\u6790\u3084\u521d\u4ee3\u57f9\u990a\u306b\u4f7f\u308f\u308c\u308b\u7d30\u80de\u89e3\u96e2\u8a66\u85ac\u306e\u9078\u629e\u30ac\u30a4\u30c9<\/a>\u300d\u306b\u307e\u3068\u3081\u3089\u308c\u3066\u3044\u307e\u3059\u306e\u3067\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002<\/p>\n<p>\u7d30\u80de\u306e\u7d99\u4ee3\u3084\u30bb\u30eb\u30ab\u30a6\u30f3\u30c8\u306b\u3064\u3044\u3066\u3082\u3063\u3068\u77e5\u308a\u305f\u3044\u65b9\u306f\u7121\u6599\u30aa\u30f3\u30e9\u30a4\u30f3\u30bb\u30df\u30ca\u30fc\u300c<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/technical-resources\/seminars\/cell-culture-basic.html\" target=\"_blank\">\u3053\u308c\u304b\u3089\u59cb\u3081\u308b\u4eba\u306f\u5fc5\u898b\uff01\u7d30\u80de\u57f9\u990a\u306e\u30ad\u30db\u30f3\u306e\u30ad<\/a>\u300d\u3001\u300c<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/technical-resources\/seminars\/cell-count-basic.html\" target=\"_blank\">\u3044\u307e\u3055\u3089\u805e\u3051\u306a\u3044\uff01\u7d30\u80de\u30ab\u30a6\u30f3\u30c8\u3092\u57fa\u672c\u304b\u3089\u5b66\u307c\u3046<\/a>\u300d\u3084\u8b1b\u7fa9\u3068\u5b9f\u7fd2\u3067\u7d30\u80de\u57f9\u990a\u306e\u57fa\u672c\u3092\u7fd2\u5f97\u3067\u304d\u308b\u7d30\u80de\u57f9\u990a\u30cf\u30f3\u30ba\u30aa\u30f3\u30c8\u30ec\u30fc\u30cb\u30f3\u30b0\uff08<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/technical-resources\/trainings\/saibo_baiyo.html\" target=\"_blank\">1\u65e5\u30b3\u30fc\u30b9<\/a>\u3001<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/technical-resources\/trainings\/saibo-baiyo2.html\" target=\"_blank\">2\u65e5\u9593\u30b3\u30fc\u30b9<\/a>\uff09\u306e\u53d7\u8b1b\u3092\u3054\u691c\u8a0e\u304f\u3060\u3055\u3044\u3002\u3053\u308c\u3089\u306e\u30c8\u30ec\u30fc\u30cb\u30f3\u30b0\u306e\u5185\u5bb9\u306e\u9055\u3044\u306b\u3064\u3044\u3066\u306f\u95a2\u9023\u8a18\u4e8b\u300c<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/cell-culture-stem-cell-bid-ts-mbr-24038\/?CID=bid_clb_r04_jp_cp0000_pjt0000_bid00000_0so_blg_cell_culture_cell_count_bid_ts_mbr_25012_Social_LAB\" target=\"_blank\">\u7d30\u80de\u57f9\u990a\u95a2\u9023\u306e\u5b66\u7fd2\u30ea\u30bd\u30fc\u30b9\u307e\u3068\u3081\uff5e\u30c8\u30ec\u30fc\u30cb\u30f3\u30b0\u30fb\u30bb\u30df\u30ca\u30fc\u30fb\u30cf\u30f3\u30c9\u30d6\u30c3\u30af\u306a\u3069\uff5e<\/a>\u300d\u3092\u53c2\u8003\u306b\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u4eca\u56de\u306e\u5185\u5bb9\u306b\u9650\u3089\u305a\u3001\u7d30\u80de\u57f9\u990a\u95a2\u9023\u306e\u88fd\u54c1\u30fb\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306a\u3069\u3067\u3054\u4e0d\u660e\u70b9\u304c\u3042\u308a\u307e\u3057\u305f\u3089\u30c6\u30af\u30cb\u30ab\u30eb\u30b5\u30dd\u30fc\u30c8\uff08<a href=\"mailto:jptech@thermofisher.com\" target=\"_blank\">jptech@thermofisher.com<\/a>\uff09\u3078\u304a\u6c17\u8efd\u306b\u304a\u554f\u3044\u5408\u308f\u305b\u304f\u3060\u3055\u3044\u3002<\/p>\n<h3>Gibco\u7d30\u80de\u57f9\u990a\u30cf\u30f3\u30c9\u30d6\u30c3\u30af\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\uff082021\u5e74\u30ea\u30cb\u30e5\u30fc\u30a2\u30eb\u7248\uff09<\/h3>\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/life-science\/gibco-cell-culture-basics-handbook.html?CID=bid_clb_r04_jp_cp0000_pjt0000_bid00000_0so_blg_cell_culture_cell_count_bid_ts_mbr_25012_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u306f\u3053\u3061\u3089<\/a><\/p>\n<p>&nbsp;<\/p>\n<p>\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u7528\u306b\u306f\u4f7f\u7528\u3044\u305f\u3060\u3051\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Trypsin-EDTA\u306f\u63a5\u7740\u7d30\u80de\u306e\u89e3\u96e2\u64cd\u4f5c\u306b\u6c4e\u7528\u3055\u308c\u3066\u3044\u308b\u8a66\u85ac\u3067\u3059\u3002\u30bf\u30f3\u30d1\u30af\u8cea\u5206\u89e3\u9175\u7d20\u3067\u3042\u308bTrypsin\u304c\u7d30\u80de\u63a5\u7740\u5206\u5b50\u3092\u6d88\u5316\u3057\u3001EDTA\u304c\u7d30\u80de\u63a5\u7740\u306b\u5fc5\u8981\u306aCa2+\u3068Mg2+\u3092\u30ad\u30ec\u30fc\u30c8\u3057\u3066\u7d30\u80de\u306e\u89e3\u96e2\u3092\u4fc3\u9032\u3057\u307e\u3059\u3002\u4e00\u822c\u306b<\/p>\n","protected":false},"author":975,"featured_media":19967,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[159],"tags":[97,23],"division":[],"class_list":{"0":"post-19938","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-cell","8":"tag-cell-count","9":"tag-cell-culture","10":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"<!-- 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