{"id":20088,"date":"2025-03-26T12:23:59","date_gmt":"2025-03-26T03:23:59","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/?p=20088"},"modified":"2026-02-18T17:38:51","modified_gmt":"2026-02-18T08:38:51","slug":"pipetting-cell-culture-bid-ts-mbr-25014","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/pipetting-cell-culture-bid-ts-mbr-25014\/","title":{"rendered":"\u3010\u3084\u3063\u3066\u307f\u305f\u3011\u7d30\u80de\u3092100\u56de\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u3057\u3066\u307f\u305f"},"content":{"rendered":"<p>\u7d30\u80de\u61f8\u6fc1\u6db2\u306e\u6df7\u5408\u306b\u306f\u30aa\u30fc\u30c8\uff08\u96fb\u52d5\uff09\u30d4\u30da\u30c3\u30bf\u30fc\u3084\u30de\u30a4\u30af\u30ed\u30d4\u30da\u30c3\u30c8\u3067\u306e\u6eb6\u6db2\u306e\u51fa\u3057\u5165\u308c\u306b\u3088\u308b\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u304c\u884c\u308f\u308c\u307e\u3059\u3002\u61f8\u6fc1\u6db2\u3092\u5747\u4e00\u306b\u3059\u308b\u305f\u3081\u306b\u5fc5\u9808\u306e\u64cd\u4f5c\u3067\u3059\u304c\u3001\u5fc5\u8981\u4ee5\u4e0a\u306e\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u3084\u6ce1\u7acb\u3064\u307b\u3069\u306e\u6fc0\u3057\u3044\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u306f\u7d30\u80de\u306b\u30c0\u30e1\u30fc\u30b8\u3092\u4e0e\u3048\u308b\u6050\u308c\u304c\u3042\u308a\u307e\u3059\u3002\u305d\u3053\u3067\u4eca\u56de\u306f\u3001HeLa\u7d30\u80de\u306e\u61f8\u6fc1\u6db2\u3092\u6700\u5927100\u56de\u6fc0\u3057\u304f\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u3057\u3001\u512a\u3057\u304f\u6700\u5c0f\u9650\u306e\u56de\u6570\u3060\u3051\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u3057\u305fHeLa\u7d30\u80de\u3068\u6bd4\u3079\u3066\u3001\u751f\u5b58\u7387\u3084\u305d\u306e\u5f8c\u306e\u5897\u6b96\u306b\u5f71\u97ff\u304c\u3042\u308b\u304b\u3069\u3046\u304b\u3092\u78ba\u8a8d\u3057\u307e\u3057\u305f\u3002<\/p>\n<h2>\u5b9f\u9a13\u65b9\u6cd5<\/h2>\n<p>\u25a0\u7d30\u80de<br \/>\nHeLa\u7d30\u80de\u306e\u61f8\u6fc1\u6db2\uff081 mL\uff09\u3092\u5165\u308c\u305f15 mL\u30c1\u30e5\u30fc\u30d62\u672c<\/p>\n<p>\u25a0\u624b\u9806<\/p>\n<ol>\n<li>1000 \u00b5L\u8a2d\u5b9a\u306e\u30de\u30a4\u30af\u30ed\u30d4\u30da\u30c3\u30c8\u3092\u7528\u3044\u3066\u3001\u7d30\u80de\u61f8\u6fc1\u6db2\u309210\u56de\u300120\u56de\u300150\u56de\u3001100\u56de\u3068\u6fc0\u3057\u304f\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\uff08\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u69d8\u5b50\u306f\u52d5\u753b1\u3092\u53c2\u7167\uff09<\/li>\n<li>Gibco\u2122 Trypan Blue\u6eb6\u6db2\uff08\u88fd\u54c1\u756a\u53f7 <a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/15250061?CID=bid_clb_r04_jp_cp0000_pjt0000_bid00000_0so_blg_pipetting_cell_culture_bid_ts_mbr_25014_Social_LAB\" target=\"_blank\">15250061<\/a>\uff09\u3068\u7d30\u80de\u61f8\u6fc1\u6db2\u30921:1\u3067\u6df7\u5408\u3057\u3066Invitrogen\u2122 Countess\u2122 Chamber Slide\uff08\u88fd\u54c1\u756a\u53f7 <a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/C10283?CID=bid_clb_r04_jp_cp0000_pjt0000_bid00000_0so_blg_pipetting_cell_culture_bid_ts_mbr_25014_Social_LAB\" target=\"_blank\">C10283<\/a>\uff09\u306b\u6ce8\u5165<\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/cell-analysis\/cell-analysis-instruments\/automated-cell-counters\/models\/countess-3-fl.html?CID=bid_clb_r04_jp_cp0000_pjt0000_bid00000_0so_blg_pipetting_cell_culture_bid_ts_mbr_25014_Social_LAB\" target=\"_blank\">Invitrogen\u2122 Countess\u2122 3 FL\u81ea\u52d5\u30bb\u30eb\u30ab\u30a6\u30f3\u30bf\u30fc<\/a>\u306e\u660e\u8996\u91ce\u30e2\u30fc\u30c9\u3067\u81ea\u52d5\u30bb\u30eb\u30ab\u30a6\u30f3\u30c8\u3057\u3066\u751f\u5b58\u7387\u3068\u7d30\u80de\u6fc3\u5ea6\u3092\u8a08\u6e2c<\/li>\n<li>\u3082\u30461\u672c\u306e\u7d30\u80de\u61f8\u6fc1\u6db2\u3092\u512a\u3057\u304f5\u56de\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\uff08\u52d5\u753b2\uff09<\/li>\n<li>\u624b\u98062\uff5e3\u3068\u540c\u69d8\u306b\u81ea\u52d5\u30bb\u30eb\u30ab\u30a6\u30f3\u30c8<\/li>\n<li>\u4e21\u65b9\u306e\u6761\u4ef6\u306e\u7d30\u80de1.5 x 10<sup>5<\/sup> cells\u30926\u30a6\u30a7\u30eb\u30d7\u30ec\u30fc\u30c8\uff08Thermo Scientific\u2122 Nunc\u2122\u7d30\u80de\u57f9\u990a\u7528\u51e6\u7406\u6e08\u307f\u30de\u30eb\u30c1\u30a6\u30a7\u30eb\u30d7\u30ec\u30fc\u30c8\u3001\u88fd\u54c1\u756a\u53f7 <a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/jp\/ja\/140675?CID=bid_clb_r04_jp_cp0000_pjt0000_bid00000_0so_blg_pipetting_cell_culture_bid_ts_mbr_25014_Social_LAB\" target=\"_blank\">140675<\/a>\uff09\u306b\u64ad\u7a2e\u3057\u30012\u65e5\u9593\u57f9\u990a<\/li>\n<li>Gibco\u2122 CTS\u2122 TrypLE\u2122 Select Enzyme\uff08\u88fd\u54c1\u756a\u53f7 <a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/jp\/ja\/A1285901?CID=bid_clb_r04_jp_cp0000_pjt0000_bid00000_0so_blg_pipetting_cell_culture_bid_ts_mbr_25014_Social_LAB\" target=\"_blank\">A1285901<\/a>\uff09\u3067\u7d30\u80de\u3092\u56de\u53ce<\/li>\n<li>\u624b\u98062\uff5e3\u3068\u540c\u69d8\u306b\u81ea\u52d5\u30bb\u30eb\u30ab\u30a6\u30f3\u30c8<\/li>\n<p><\/p>\n<div style=\"text-align: center\">\n<div style=\"margin-bottom: 5px\">\u25bc \u52d5\u753b1. \u6fc0\u3057\u3044\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u69d8\u5b50<\/div>\n<figure class=\"wp-block-video\"><video src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/03\/01_strong.mp4\" controls=\"controls\" width=\"640\"><\/video><\/figure>\n<\/div>\n<p><\/p>\n<div style=\"text-align: center\">\n<div style=\"margin-bottom: 5px\">\u25bc \u52d5\u753b2. \u512a\u3057\u3044\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u69d8\u5b50<\/div>\n<figure class=\"wp-block-video\"><video src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/03\/02_gentle.mp4\" controls=\"controls\" width=\"640\"><\/video><\/figure>\n<\/div>\n<\/ol>\n<h2>\u6fc0\u3057\u3044\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u304c\u7d30\u80de\u306b\u4e0e\u3048\u308b\u5f71\u97ff<\/h2>\n<p>\u3055\u3063\u305d\u304f\u7d50\u679c\u3092\u898b\u3066\u3044\u304d\u307e\u3057\u3087\u3046\u3002HeLa\u7d30\u80de\u306e\u61f8\u6fc1\u6db2\u3092\u7d2f\u8a0810\u56de\u300120\u56de\u300150\u56de\u3001100\u56de\u3068\u6fc0\u3057\u304f\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u3057\u305f\u5f8c\u306e\u751f\u5b58\u7387\u304c\u56f31\u3067\u3059\u3002\u3053\u306e\u7d50\u679c\u3092\u898b\u308b\u9650\u308a\u3001100\u56de\u6fc0\u3057\u304f\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u3057\u3066\u3082HeLa\u7d30\u80de\u306e\u751f\u5b58\u7387\u306b\u306f\u5f71\u97ff\u3057\u306a\u3044\u3053\u3068\u304c\u308f\u304b\u308a\u307e\u3057\u305f\u3002<\/p>\n<div id=\"attachment_20101\" style=\"width: 410px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-20101\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/03\/13d978127778a535806fb207260c4eba.jpg\" alt=\"\" width=\"400\" height=\"362\" class=\"size-full wp-image-20101\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/03\/13d978127778a535806fb207260c4eba.jpg 997w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/03\/13d978127778a535806fb207260c4eba-300x271.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/03\/13d978127778a535806fb207260c4eba-768x695.jpg 768w\" sizes=\"auto, (max-width: 400px) 100vw, 400px\" \/><p id=\"caption-attachment-20101\" class=\"wp-caption-text\">\u56f31.  \u6fc0\u3057\u3044\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u306b\u3088\u308b\u7d30\u80de\u306e\u751f\u5b58\u7387\u3078\u306e\u5f71\u97ff<\/p><\/div>\n<p>\u4ee5\u524d\u306e\u691c\u8a3c\u3067\u3001\u4f55\u3089\u304b\u306e\u64cd\u4f5c\u306b\u3088\u308b\u7d30\u80de\u306e\u751f\u5b58\u3078\u306e\u5f71\u97ff\u3092\u6e2c\u5b9a\u3059\u308b\u969b\u306b\u306f\u3001Trypan Blue\u306b\u3088\u308b\u751f\u6b7b\u5224\u5b9a\u3060\u3051\u3067\u306a\u304f\u3001\u751f\u7d30\u80de\u306e\u6fc3\u5ea6\uff08\u6570\uff09\u306e\u6e2c\u5b9a\u3082\u91cd\u8981\u3067\u3042\u308b\u3053\u3068\u304c\u308f\u304b\u308a\u307e\u3057\u305f\uff08\u95a2\u9023\u8a18\u4e8b\uff1a<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/cell-culture-cell-count-bid-ts-mbr-25012\/?CID=bid_clb_r04_jp_cp0000_pjt0000_bid00000_0so_blg_pipetting_cell_culture_bid_ts_mbr_25014_Social_LAB\" target=\"_blank\">\u3010\u3084\u3063\u3066\u307f\u305f\u3011\u201d\u3084\u3055\u3057\u3044\u201d\u7d30\u80de\u89e3\u96e2\u8a66\u85ac\u300cTrypLE\u300d\u3067\u7d30\u80de\u3092\u304d\u3073\u3057\u304f\u51e6\u7406\u3057\u3066\u307f\u305f<\/a>\uff09\u3002\u305d\u3053\u3067\u3001\u751f\u7d30\u80de\u306e\u6fc3\u5ea6\u3092\u7b97\u51fa\u3057\u3066\u56f32\u306b\u307e\u3068\u3081\u307e\u3057\u305f\u3002\u305d\u306e\u7d50\u679c\u3001\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b050\u56de\u3001100\u56de\u306e\u751f\u7d30\u80de\u6fc3\u5ea6\u304c\u6570\u5b57\u4e0a\u306f\u308f\u305a\u304b\u306b\u4f4e\u4e0b\u3057\u305f\u3082\u306e\u306e\u3001\u6709\u610f\u306a\u5dee\u306f\u898b\u3089\u308c\u306a\u3044\u304c\u308f\u304b\u308a\u307e\u3057\u305f\u3002<\/p>\n<div id=\"attachment_20108\" style=\"width: 410px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-20108\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/03\/9b66e46de3f32e1889ce11b28e7a5f49.jpg\" alt=\"\" width=\"400\" height=\"362\" class=\"size-full wp-image-20108\" \/><p id=\"caption-attachment-20108\" class=\"wp-caption-text\">\u56f32. 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id=\"attachment_20110\" style=\"width: 410px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-20110\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/03\/12988bd312ade347182bd1d2bf918549.jpg\" alt=\"\" width=\"400\" height=\"362\" class=\"size-full wp-image-20110\" \/><p id=\"caption-attachment-20110\" class=\"wp-caption-text\">\u56f33. 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href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/technical-resources\/trainings\/saibo_baiyo.html?CID=bid_clb_r04_jp_cp0000_pjt0000_bid00000_0so_blg_pipetting_cell_culture_bid_ts_mbr_25014_Social_LAB\" target=\"_blank\">1\u65e5\u30b3\u30fc\u30b9<\/a>\u3001<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/technical-resources\/trainings\/saibo_baiyo.html?CID=bid_clb_r04_jp_cp0000_pjt0000_bid00000_0so_blg_pipetting_cell_culture_bid_ts_mbr_25014_Social_LAB\" target=\"_blank\">2\u65e5\u9593\u30b3\u30fc\u30b9<\/a>\uff09\u306e\u53d7\u8b1b\u3092\u3054\u691c\u8a0e\u304f\u3060\u3055\u3044\u3002\u3053\u308c\u3089\u306e\u30c8\u30ec\u30fc\u30cb\u30f3\u30b0\u306e\u5185\u5bb9\u306e\u9055\u3044\u306b\u3064\u3044\u3066\u306f<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/cell-culture-stem-cell-bid-ts-mbr-24038\/?CID=bid_clb_r04_jp_cp0000_pjt0000_bid00000_0so_blg_pipetting_cell_culture_bid_ts_mbr_25014_Social_LAB\" target=\"_blank\">\u95a2\u9023\u8a18\u4e8b<\/a>\u3092\u53c2\u8003\u306b\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u307e\u305f\u3001\u30d0\u30a4\u30aa\u5b9f\u9a13\u3092\u3053\u308c\u304b\u3089\u59cb\u3081\u308b\u65b9\u306b\u306f\u30cf\u30f3\u30ba\u30aa\u30f3\u30c8\u30ec\u30fc\u30cb\u30f3\u30b0\u300c<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/technical-resources\/trainings\/bio-experiment.html?CID=bid_clb_r04_jp_cp0000_pjt0000_bid00000_0so_blg_pipetting_cell_culture_bid_ts_mbr_25014_Social_LAB\" target=\"_blank\">\u3053\u308c\u304b\u3089\u59cb\u3081\u308b\u30d0\u30a4\u30aa\u5b9f\u9a13 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