{"id":201,"date":"2015-12-01T00:00:00","date_gmt":"2015-12-01T00:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic16\/"},"modified":"2025-04-17T15:10:24","modified_gmt":"2025-04-17T06:10:24","slug":"qpcr-basic16","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic16\/","title":{"rendered":"\u691c\u91cf\u7dda\u3092\u3072\u304b\u305a\u306b\u5b9a\u91cf\u3059\u308b\u6bd4\u8f03Ct\u6cd5\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u8a2d\u8a08\uff5c\u4eca\u3053\u305d\u672c\u6c17\u3067\u5fb9\u5e95\u7406\u89e3! \u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u8b1b\u5ea7 \u7b2c16\u56de"},"content":{"rendered":"

\u6bd4\u8f03Ct\u6cd5\u306e\u5b9f\u8df5\u7de8\u3082\u3001\u3044\u3088\u3044\u3088\u6700\u7d42\u56de\u3002\u4eca\u56de\u306f\u3001\u6bd4\u8f03Ct\u6cd5\u3092\u3064\u304b\u3044\u3053\u306a\u3059\u305f\u3081\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u8a2d\u8a08\u306a\u3069\u3001\u91cd\u8981\u306a\u30dd\u30a4\u30f3\u30c8\u3092\u6574\u7406\u3057\u3066\u3044\u304d\u307e\u3059\u3002<\/p>\n

PCR\u52b9\u7387\u306e\u7b49\u3057\u3044\u30d7\u30e9\u30a4\u30de\u30fc\u304c\u5fc5\u9808\u3068\u306a\u308b\u6bd4\u8f03Ct\u6cd5\u3002\u3064\u307e\u308a\u6bd4\u8f03Ct\u6cd5\u3067\u306f\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u8a2d\u8a08\u304c\u91cd\u8981\u306a\u30dd\u30a4\u30f3\u30c8\u3068\u306a\u308b<\/strong>\u3053\u3068\u304c\u308f\u304b\u308a\u307e\u3059\u3002\u305d\u3053\u3067\u3054\u7d39\u4ecb\u3057\u305f\u3044\u306e\u304c\u3001\u5f0a\u793e\u306e\u88fd\u54c1\u7fa4\u3067\u3059\u3002Primer Express\u00ae \u30bd\u30d5\u30c8\u30a6\u30a7\u30a2<\/a>\u3001TaqMan\u00ae Gene Expression Assays<\/a>\u306a\u3069\u306e\u907a\u4f1d\u5b50\u767a\u73fe\u89e3\u6790\u8a66\u85ac\u985e\u306f\u3001\u826f\u597d\u306aPCR\u5897\u5e45\u52b9\u7387\u304c\u5f97\u3089\u308c\u308b\u30d7\u30e9\u30a4\u30de\u30fc\u8a2d\u8a08\u3092\u76ee\u7684\u306b\u3001\u53b3\u5bc6\u306b\u30c7\u30b6\u30a4\u30f3\u3055\u308c\u3066\u3044\u307e\u3059\u3002<\/strong>\u307e\u305fRQ(Relative Quantification)\u306f\u3001\u5b9f\u9a13\u30c7\u30fc\u30bf\u3092\u4ed6\u306e\u8a08\u7b97\u7528\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u306b\u30a8\u30af\u30b9\u30dd\u30fc\u30c8\u3059\u308b\u3053\u3068\u306a\u304f\u6bd4\u8f03Ct\u6cd5\u304c\u5b9f\u884c\u3067\u304d\u308b\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2<\/strong>\u3067\u3059\u30021\u679a\u306e\u30d7\u30ec\u30fc\u30c8\u5185\u306e\u30b5\u30f3\u30d7\u30eb\u30c7\u30fc\u30bf\u3060\u3051\u3067\u306a\u304f\u3001\u540c\u4e00\u306e\u6761\u4ef6\u3067\u6e2c\u5b9a\u3057\u305f\u7570\u306a\u308b\u30d7\u30ec\u30fc\u30c8\u9593\u306e\u30c7\u30fc\u30bf\u3082\u89e3\u6790\u3067\u304d\u307e\u3059\uff08\u56f3\uff09\u3002<\/p>\n

\"qpcr-basic16-fig\"<\/a>

\u56f3 Relative Quantification\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u306b\u3088\u308b\u6bd4\u8f03Ct\u6cd5\u306e\u89e3\u6790\u7d50\u679c\u753b\u9762<\/p><\/div>\n

\u56f3\u306f\u3001\u809d\u81d3\uff08\u8d64\uff09\u3001\u8180\u80f1\uff08\u7dd1\uff09\u3001\u814e\u81d3\uff08\u9752\uff09\u3092\u30b5\u30f3\u30d7\u30eb\u3068\u3057\u3066\u300110\u7a2e\u985e\u306e\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u3092\u6bd4\u8f03Ct\u6cd5\u3067\u76f8\u5bfe\u5b9a\u91cf\u3057\u305f\u7d50\u679c\u3092\u793a\u3057\u3066\u3044\u307e\u3059\u3002\u30b0\u30e9\u30d5\u306e\u6a2a\u8ef8\u306f\u5404\u907a\u4f1d\u5b50\u306e\u76f8\u5bfe\u7684\u767a\u73fe\u91cf\u3067\u3001\u809d\u81d3\u3092\u30ad\u30e3\u30ea\u30d6\u30ec\u30fc\u30bf\u30fc\u3068\u3057\u3066Log\u8868\u793a\u3057\u3066\u3044\u307e\u3059\u3002\u4f8b\u3048\u3070\u3001RARG\u907a\u4f1d\u5b50\u306f\u809d\u81d3\u306b\u5bfe\u3057\u3066\u8180\u80f1\u3067\u306f101.32<\/sup> =20.1\u500d\u3001\u814e\u81d3\u3067\u306f10-0.71<\/sup> =0.19\u500d\u767a\u73fe\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u308f\u304b\u308a\u307e\u3059\u3002\u7d50\u679c\u306e\u8a73\u7d30\u306f\u5de6\u306e\u30ab\u30e9\u30e0\u306b\u307e\u3068\u3081\u3066\u8868\u793a\u3055\u308c\u307e\u3059\u3002\u8a73\u3057\u304f\u306f\u53c2\u8003\u8cc7\u6599\u3092\u3054\u53c2\u8003\u304f\u3060\u3055\u3044\uff08\u53c2\u8003\u8cc7\u65992\uff09\u3002
\n\n\t

\n\t\t\u3068\u3066\u3082\u4fbf\u5229\u306a\u6bd4\u8f03Ct\u6cd5\u3067\u3059\u304c\u3001\u3042\u304f\u307e\u3067\u3082\u6700\u3082\u6b63\u78ba\u306a\u5b9a\u91cf\u65b9\u6cd5\u306f\u691c\u91cf\u7dda\u6cd5\u3067\u3042\u308b\u3053\u3068\u3092\u5fd8\u308c\u306a\u3044\u3067\u304f\u3060\u3055\u3044\u3002\u307f\u306a\u3055\u307e\u306b\u306f\u5b9f\u9a13\u30c7\u30fc\u30bf\u306b\u4f55\u3092\u6c42\u3081\u308b\u304b\u306b\u3088\u3063\u3066\u300c\u6bd4\u8f03Ct\u6cd5\u300d\u300c\u691c\u91cf\u7dda\u6cd5\u300d\u306e2\u3064\u306e\u65b9\u6cd5\u3092\u52b9\u7387\u826f\u304f\u4f7f\u3044\u5206\u3051\u3001\u6b63\u3057\u3044\u7814\u7a76\u7d50\u679c\u3092\u5c0e\u3044\u3066\u3044\u305f\u3060\u304d\u305f\u3044\u3068\u601d\u3044\u307e\u3059\u3002\u8ad6\u6587\u57f7\u7b46\u306e\u969b\u306f\u3001Monney\u3089\uff08\u53c2\u8003\u8cc7\u65992\uff09\u3001Mullen\u3089\uff08\u53c2\u8003\u8cc7\u65993\uff09\u306b\u3088\u308b\u6bd4\u8f03\u5b9a\u91cf\u6cd5\u306b\u3064\u3044\u3066\u306e\u8003\u5bdf\u3092\u3001\u305c\u3072\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002\t<\/blockquote>\n\n\t

<\/p>\n\t\n\t
\n\u306a\u308b\u307b\u3069\u30012\u3064\u306e\u65b9\u6cd5\u306e\u30e1\u30ea\u30c3\u30c8\u30fb\u30c7\u30e1\u30ea\u30c3\u30c8\u3092\u3057\u3063\u304b\u308a\u898b\u6975\u3081\u3001\u5b9f\u9a13\u8a08\u753b\u3092\u7acb\u3066\u3066\u3044\u304d\u305f\u3044\u3067\u3059\u306d\u3002<\/p>\n

\u53c2\u8003\u8cc7\u6599<\/h2>\n
    \n
  1. Comparative Ct\u6cd5\u306b\u3088\u308b\u76f8\u5bfe\u767a\u73fe\u5b9a\u91cf\u8cc7\u6599<\/a>\uff08ABI PRISM 7000\u30b7\u30b9\u30c6\u30e0\u7528\uff09<\/li>\n
  2. Th1-specific cell surface protein Tim-3 regulates macrophage activation and severity of an autoimmune disease.\u3000Monney L, Sabatos CA, Gaglia JL, Ryu A, Waldner H, Chernova T, Manning S, Greenfield EA, Coyle AJ, Sobel RA, Freeman GJ, Kuchroo VK.\u3000Nature. 2002 Jan 31;415(6871):536-41.\u3010Medline<\/a>\u3011<\/li>\n
  3. Hlx is induced by and genetically interacts with T-bet to promote heritable T(H)1 gene induction.\u3000Mullen AC, Hutchins AS, High FA, Lee HW, Sykes KJ, Chodosh LA, Reiner SL.\u3000 Nat Immunol. 2002 Jul;3(7):652-8. Epub 2002 Jun 10.\u3000 \u3010Medline<\/a>\u3011<\/li>\n<\/ol>\n

    \u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u30cf\u30f3\u30c9\u30d6\u30c3\u30af \u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9<\/h2>\n

    \u3053\u306e\u30cf\u30f3\u30c9\u30d6\u30c3\u30af\u3067\u306f\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u7406\u8ad6\u3084\u5b9f\u9a13\u30c7\u30b6\u30a4\u30f3\u306e\u8a2d\u8a08\u306a\u3069\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u57fa\u790e\u77e5\u8b58\u304c\u63b2\u8f09\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3092\u59cb\u3081\u305f\u3070\u304b\u308a\u306e\u65b9\u3084\u3053\u308c\u304b\u3089\u5b9f\u9a13\u3092\u8003\u3048\u3066\u3044\u308b\u65b9\u306b\u3046\u3063\u3066\u3064\u3051\u306e\u30cf\u30f3\u30c9\u30d6\u30c3\u30af\u3067\u3059\u3002PDF\u30d5\u30a1\u30a4\u30eb\u306e\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3092\u3054\u5e0c\u671b\u306e\u65b9\u306f\u3001\u4e0b\u8a18\u30dc\u30bf\u30f3\u3088\u308a\u304a\u7533\u3057\u8fbc\u307f\u304f\u3060\u3055\u3044\u3002
    \n    \u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/p>\n

    \u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u30c8\u30e9\u30d6\u30eb\u30b7\u30e5\u30fc\u30c6\u30a3\u30f3\u30b0 \u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9<\/h2>\n

    \u7570\u5e38\u306aS\u5b57\u72b6\u306e\u5897\u5e45\u66f2\u7dda\u3001NTC\u3067\u306e\u5897\u5e45\u691c\u51fa\u3001\u5897\u5e45\u304c\u898b\u3089\u308c\u306a\u3044\u306a\u3069\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u5b9f\u9a13\u4e2d\u306b\u56f0\u3063\u305f\u969b\u306e\u624b\u52a9\u3051\u306b\u306a\u308b\u60c5\u5831\u3092\u7d39\u4ecb\u3057\u3066\u3044\u307e\u3059\u3002PDF\u30d5\u30a1\u30a4\u30eb\u306e\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3092\u3054\u5e0c\u671b\u306e\u65b9\u306f\u3001\u4e0b\u8a18\u30dc\u30bf\u30f3\u3088\u308a\u304a\u7533\u3057\u8fbc\u307f\u304f\u3060\u3055\u3044\u3002<\/p>\n

    \u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/p>\n

     <\/p>\n

    \u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u7528\u306b\u306f\u4f7f\u7528\u3044\u305f\u3060\u3051\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

    \u300c\u6bd4\u8f03Ct\u6cd5\u300d\u3092\u6210\u529f\u3055\u305b\u308b\u30dd\u30a4\u30f3\u30c8\u304c\u3001\u6700\u521d\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u8a2d\u8a08\u3002\u3067\u306f\u3001\u6bd4\u8f03Ct\u6cd5\u306b\u9069\u3057\u305f\u30d7\u30e9\u30a4\u30de\u30fc\u8a2d\u8a08\u306b\u5f79\u7acb\u3064\u8a66\u85ac\u30fb\u30bd\u30d5\u30c8\u30a6\u30a8\u30a2\u306b\u306f\u3001\u3069\u3093\u306a\u3082\u306e\u304c\u3042\u308b\u306e\u3067\u3057\u3087\u3046\u3002\u304a\u3059\u3059\u3081\u88fd\u54c1\u7fa4\u3092\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n","protected":false},"author":988,"featured_media":2321,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[156],"tags":[7],"division":[],"class_list":{"0":"post-201","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-mol-bio","8":"tag-qpcr","9":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"\n\u691c\u91cf\u7dda\u3092\u3072\u304b\u305a\u306b\u5b9a\u91cf\u3059\u308b\u6bd4\u8f03Ct\u6cd5\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u8a2d\u8a08\uff5c\u4eca\u3053\u305d\u672c\u6c17\u3067\u5fb9\u5e95\u7406\u89e3! \u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u8b1b\u5ea7 \u7b2c16\u56de - Learning at the Bench<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic16\/\" \/>\n<meta property=\"og:locale\" content=\"ja_JP\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"\u691c\u91cf\u7dda\u3092\u3072\u304b\u305a\u306b\u5b9a\u91cf\u3059\u308b\u6bd4\u8f03Ct\u6cd5\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u8a2d\u8a08\uff5c\u4eca\u3053\u305d\u672c\u6c17\u3067\u5fb9\u5e95\u7406\u89e3! \u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u8b1b\u5ea7 \u7b2c16\u56de\" \/>\n<meta property=\"og:description\" content=\"\u300c\u6bd4\u8f03Ct\u6cd5\u300d\u3092\u6210\u529f\u3055\u305b\u308b\u30dd\u30a4\u30f3\u30c8\u304c\u3001\u6700\u521d\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u8a2d\u8a08\u3002\u3067\u306f\u3001\u6bd4\u8f03Ct\u6cd5\u306b\u9069\u3057\u305f\u30d7\u30e9\u30a4\u30de\u30fc\u8a2d\u8a08\u306b\u5f79\u7acb\u3064\u8a66\u85ac\u30fb\u30bd\u30d5\u30c8\u30a6\u30a8\u30a2\u306b\u306f\u3001\u3069\u3093\u306a\u3082\u306e\u304c\u3042\u308b\u306e\u3067\u3057\u3087\u3046\u3002\u304a\u3059\u3059\u3081\u88fd\u54c1\u7fa4\u3092\u7d39\u4ecb\u3057\u307e\u3059\u3002\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic16\/\" \/>\n<meta property=\"og:site_name\" content=\"Learning at the Bench\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/ThermoFisherJapan\" \/>\n<meta property=\"article:published_time\" content=\"2015-12-01T00:00:00+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2025-04-17T06:10:24+00:00\" \/>\n<meta property=\"og:image\" content=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic-title-15.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1200\" \/>\n\t<meta property=\"og:image:height\" content=\"630\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"LATB Staff\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@ThermoFisherJP\" \/>\n<meta name=\"twitter:site\" content=\"@ThermoFisherJP\" \/>\n<meta name=\"twitter:label1\" content=\"\u57f7\u7b46\u8005\" \/>\n\t<meta name=\"twitter:data1\" content=\"LATB Staff\" \/>\n\t<meta name=\"twitter:label2\" content=\"\u63a8\u5b9a\u8aad\u307f\u53d6\u308a\u6642\u9593\" \/>\n\t<meta name=\"twitter:data2\" content=\"6\u5206\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/qpcr-basic16\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/qpcr-basic16\\\/\"},\"author\":{\"name\":\"LATB Staff\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/#\\\/schema\\\/person\\\/00a095a01d54afcdf9457465feb04f7d\"},\"headline\":\"\u691c\u91cf\u7dda\u3092\u3072\u304b\u305a\u306b\u5b9a\u91cf\u3059\u308b\u6bd4\u8f03Ct\u6cd5\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u8a2d\u8a08\uff5c\u4eca\u3053\u305d\u672c\u6c17\u3067\u5fb9\u5e95\u7406\u89e3! \u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u8b1b\u5ea7 \u7b2c16\u56de\",\"datePublished\":\"2015-12-01T00:00:00+00:00\",\"dateModified\":\"2025-04-17T06:10:24+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/qpcr-basic16\\\/\"},\"wordCount\":140,\"commentCount\":0,\"image\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/qpcr-basic16\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/wp-content\\\/uploads\\\/sites\\\/13\\\/2019\\\/07\\\/qpcr-basic-title-15.jpg\",\"keywords\":[\"\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\"],\"articleSection\":[\"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023\"],\"inLanguage\":\"ja\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/qpcr-basic16\\\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/qpcr-basic16\\\/\",\"url\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/qpcr-basic16\\\/\",\"name\":\"\u691c\u91cf\u7dda\u3092\u3072\u304b\u305a\u306b\u5b9a\u91cf\u3059\u308b\u6bd4\u8f03Ct\u6cd5\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u8a2d\u8a08\uff5c\u4eca\u3053\u305d\u672c\u6c17\u3067\u5fb9\u5e95\u7406\u89e3! \u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u8b1b\u5ea7 \u7b2c16\u56de - Learning at the Bench\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/qpcr-basic16\\\/#primaryimage\"},\"image\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/qpcr-basic16\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/wp-content\\\/uploads\\\/sites\\\/13\\\/2019\\\/07\\\/qpcr-basic-title-15.jpg\",\"datePublished\":\"2015-12-01T00:00:00+00:00\",\"dateModified\":\"2025-04-17T06:10:24+00:00\",\"author\":{\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/#\\\/schema\\\/person\\\/00a095a01d54afcdf9457465feb04f7d\"},\"breadcrumb\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/qpcr-basic16\\\/#breadcrumb\"},\"inLanguage\":\"ja\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/qpcr-basic16\\\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"ja\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/qpcr-basic16\\\/#primaryimage\",\"url\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/wp-content\\\/uploads\\\/sites\\\/13\\\/2019\\\/07\\\/qpcr-basic-title-15.jpg\",\"contentUrl\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/wp-content\\\/uploads\\\/sites\\\/13\\\/2019\\\/07\\\/qpcr-basic-title-15.jpg\",\"width\":1200,\"height\":630,\"caption\":\"qpcr-basic-title\"},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/qpcr-basic16\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"\u691c\u91cf\u7dda\u3092\u3072\u304b\u305a\u306b\u5b9a\u91cf\u3059\u308b\u6bd4\u8f03Ct\u6cd5\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u8a2d\u8a08\uff5c\u4eca\u3053\u305d\u672c\u6c17\u3067\u5fb9\u5e95\u7406\u89e3! \u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u8b1b\u5ea7 \u7b2c16\u56de\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/#website\",\"url\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/\",\"name\":\"Learning at the Bench\",\"description\":\"\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"ja\"},{\"@type\":\"Person\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/#\\\/schema\\\/person\\\/00a095a01d54afcdf9457465feb04f7d\",\"name\":\"LATB Staff\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"ja\",\"@id\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/8803277856c8b753feb4217dff0f9953327452df1a615c3c88b2f9864efb2ddd?s=96&d=mm&r=g\",\"url\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/8803277856c8b753feb4217dff0f9953327452df1a615c3c88b2f9864efb2ddd?s=96&d=mm&r=g\",\"contentUrl\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/8803277856c8b753feb4217dff0f9953327452df1a615c3c88b2f9864efb2ddd?s=96&d=mm&r=g\",\"caption\":\"LATB Staff\"},\"url\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/author\\\/latbstaff\\\/\"}]}<\/script>\n<!-- \/ Yoast SEO Premium plugin. -->","yoast_head_json":{"title":"\u691c\u91cf\u7dda\u3092\u3072\u304b\u305a\u306b\u5b9a\u91cf\u3059\u308b\u6bd4\u8f03Ct\u6cd5\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u8a2d\u8a08\uff5c\u4eca\u3053\u305d\u672c\u6c17\u3067\u5fb9\u5e95\u7406\u89e3! \u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u8b1b\u5ea7 \u7b2c16\u56de - Learning at the Bench","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic16\/","og_locale":"ja_JP","og_type":"article","og_title":"\u691c\u91cf\u7dda\u3092\u3072\u304b\u305a\u306b\u5b9a\u91cf\u3059\u308b\u6bd4\u8f03Ct\u6cd5\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u8a2d\u8a08\uff5c\u4eca\u3053\u305d\u672c\u6c17\u3067\u5fb9\u5e95\u7406\u89e3! \u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u8b1b\u5ea7 \u7b2c16\u56de","og_description":"\u300c\u6bd4\u8f03Ct\u6cd5\u300d\u3092\u6210\u529f\u3055\u305b\u308b\u30dd\u30a4\u30f3\u30c8\u304c\u3001\u6700\u521d\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u8a2d\u8a08\u3002\u3067\u306f\u3001\u6bd4\u8f03Ct\u6cd5\u306b\u9069\u3057\u305f\u30d7\u30e9\u30a4\u30de\u30fc\u8a2d\u8a08\u306b\u5f79\u7acb\u3064\u8a66\u85ac\u30fb\u30bd\u30d5\u30c8\u30a6\u30a8\u30a2\u306b\u306f\u3001\u3069\u3093\u306a\u3082\u306e\u304c\u3042\u308b\u306e\u3067\u3057\u3087\u3046\u3002\u304a\u3059\u3059\u3081\u88fd\u54c1\u7fa4\u3092\u7d39\u4ecb\u3057\u307e\u3059\u3002","og_url":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic16\/","og_site_name":"Learning at the Bench","article_publisher":"https:\/\/www.facebook.com\/ThermoFisherJapan","article_published_time":"2015-12-01T00:00:00+00:00","article_modified_time":"2025-04-17T06:10:24+00:00","og_image":[{"width":1200,"height":630,"url":"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic-title-15.jpg","type":"image\/jpeg"}],"author":"LATB Staff","twitter_card":"summary_large_image","twitter_creator":"@ThermoFisherJP","twitter_site":"@ThermoFisherJP","twitter_misc":{"\u57f7\u7b46\u8005":"LATB Staff","\u63a8\u5b9a\u8aad\u307f\u53d6\u308a\u6642\u9593":"6\u5206"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic16\/#article","isPartOf":{"@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic16\/"},"author":{"name":"LATB Staff","@id":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/#\/schema\/person\/00a095a01d54afcdf9457465feb04f7d"},"headline":"\u691c\u91cf\u7dda\u3092\u3072\u304b\u305a\u306b\u5b9a\u91cf\u3059\u308b\u6bd4\u8f03Ct\u6cd5\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u8a2d\u8a08\uff5c\u4eca\u3053\u305d\u672c\u6c17\u3067\u5fb9\u5e95\u7406\u89e3! \u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u8b1b\u5ea7 \u7b2c16\u56de","datePublished":"2015-12-01T00:00:00+00:00","dateModified":"2025-04-17T06:10:24+00:00","mainEntityOfPage":{"@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic16\/"},"wordCount":140,"commentCount":0,"image":{"@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic16\/#primaryimage"},"thumbnailUrl":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic-title-15.jpg","keywords":["\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR"],"articleSection":["\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023"],"inLanguage":"ja","potentialAction":[{"@type":"CommentAction","name":"Comment","target":["https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic16\/#respond"]}]},{"@type":"WebPage","@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic16\/","url":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic16\/","name":"\u691c\u91cf\u7dda\u3092\u3072\u304b\u305a\u306b\u5b9a\u91cf\u3059\u308b\u6bd4\u8f03Ct\u6cd5\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u8a2d\u8a08\uff5c\u4eca\u3053\u305d\u672c\u6c17\u3067\u5fb9\u5e95\u7406\u89e3! \u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u8b1b\u5ea7 \u7b2c16\u56de - Learning at the Bench","isPartOf":{"@id":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/#website"},"primaryImageOfPage":{"@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic16\/#primaryimage"},"image":{"@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic16\/#primaryimage"},"thumbnailUrl":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic-title-15.jpg","datePublished":"2015-12-01T00:00:00+00:00","dateModified":"2025-04-17T06:10:24+00:00","author":{"@id":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/#\/schema\/person\/00a095a01d54afcdf9457465feb04f7d"},"breadcrumb":{"@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic16\/#breadcrumb"},"inLanguage":"ja","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic16\/"]}]},{"@type":"ImageObject","inLanguage":"ja","@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic16\/#primaryimage","url":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic-title-15.jpg","contentUrl":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic-title-15.jpg","width":1200,"height":630,"caption":"qpcr-basic-title"},{"@type":"BreadcrumbList","@id":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic16\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/"},{"@type":"ListItem","position":2,"name":"\u691c\u91cf\u7dda\u3092\u3072\u304b\u305a\u306b\u5b9a\u91cf\u3059\u308b\u6bd4\u8f03Ct\u6cd5\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u8a2d\u8a08\uff5c\u4eca\u3053\u305d\u672c\u6c17\u3067\u5fb9\u5e95\u7406\u89e3! \u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u8b1b\u5ea7 \u7b2c16\u56de"}]},{"@type":"WebSite","@id":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/#website","url":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/","name":"Learning at the Bench","description":"","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"ja"},{"@type":"Person","@id":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/#\/schema\/person\/00a095a01d54afcdf9457465feb04f7d","name":"LATB Staff","image":{"@type":"ImageObject","inLanguage":"ja","@id":"https:\/\/secure.gravatar.com\/avatar\/8803277856c8b753feb4217dff0f9953327452df1a615c3c88b2f9864efb2ddd?s=96&d=mm&r=g","url":"https:\/\/secure.gravatar.com\/avatar\/8803277856c8b753feb4217dff0f9953327452df1a615c3c88b2f9864efb2ddd?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/8803277856c8b753feb4217dff0f9953327452df1a615c3c88b2f9864efb2ddd?s=96&d=mm&r=g","caption":"LATB Staff"},"url":"https:\/\/admin.acceleratingscience.com\/author\/latbstaff\/"}]}},"taxonomy_info":{"category":[{"value":156,"label":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023"}],"post_tag":[{"value":7,"label":"\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR"}]},"featured_image_src_large":["https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic-title-15-1024x538.jpg",760,399,true],"author_info":{"display_name":"LATB Staff","author_link":"https:\/\/admin.acceleratingscience.com\/author\/latbstaff\/"},"comment_info":0,"category_info":[{"term_id":156,"name":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","slug":"mol-bio","term_group":0,"term_taxonomy_id":156,"taxonomy":"category","description":"","parent":0,"count":536,"filter":"raw","meta":[],"cat_ID":156,"category_count":536,"category_description":"","cat_name":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","category_nicename":"mol-bio","category_parent":0}],"tag_info":[{"term_id":7,"name":"\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR","slug":"qpcr","term_group":0,"term_taxonomy_id":7,"taxonomy":"post_tag","description":"","parent":0,"count":91,"filter":"raw","meta":[]}],"jetpack-related-posts":[{"id":25247,"url":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/exosome-gene-expression-ts-r-25018\/","url_meta":{"origin":201,"position":0},"title":"\u3010\u7b2c4\u56de\u3011”RNA cargo”\u306e\u7cbe\u88fd\u3068\u5b9a\u91cf\u89e3\u6790\uff5emiRNA\u306e\u5b9a\u91cf\u89e3\u6790\uff5e","author":"LatB Staff","date":"2026\u5e745\u670829\u65e5","format":false,"excerpt":"\u524d\u56de\uff08\u3010\u7b2c3\u56de\u3011\u201cRNA cargo\u201d\u306e\u7cbe\u88fd\u3068\u5b9a\u91cf\u89e3\u6790\uff09\u3067\u306f\u3001\u30a8\u30af\u30bd\u30bd\u30fc\u30e0 \uff08\u30a8\u30ad\u30bd\u30bd\u30fc\u30e0\uff09 \u304b\u3089\u306e\u2026","rel":"","context":"\u5206\u6790\u30fb\u6e2c\u5b9a\u95a2\u9023","block_context":{"text":"\u5206\u6790\u30fb\u6e2c\u5b9a\u95a2\u9023","link":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/analysis\/"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/05\/exosome-gene-expression-ts-r-25018_eyecatch-1.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/05\/exosome-gene-expression-ts-r-25018_eyecatch-1.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/05\/exosome-gene-expression-ts-r-25018_eyecatch-1.jpg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/05\/exosome-gene-expression-ts-r-25018_eyecatch-1.jpg?resize=700%2C400&ssl=1 2x"},"classes":[]},{"id":25325,"url":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/exosome-gene-expression-ts-r-25019\/","url_meta":{"origin":201,"position":1},"title":"\u3010\u7b2c5\u56de\u3011 “RNA cargo”\u306e\u7cbe\u88fd\u3068\u5b9a\u91cf\u89e3\u6790\u300d\uff5emRNA\u306e\u5b9a\u91cf\u89e3\u6790\uff5e","author":"LatB Staff","date":"2026\u5e746\u670811\u65e5","format":false,"excerpt":"\u4eca\u56de\u306f\u3001\u30a8\u30af\u30bd\u30bd\u30fc\u30e0\uff08\u30a8\u30ad\u30bd\u30bd\u30fc\u30e0\uff09\u4e2d\u306emRNA\u306e\u5b9a\u91cf\u89e3\u6790\u306b\u3064\u3044\u3066\u8aac\u660e\u3057\u307e\u3059\u3002\u7b2c3\u56de\u3067\u30a8\u30af\u30bd\u30bd\u30fc\u30e0\u304b\u2026","rel":"","context":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","block_context":{"text":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","link":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/mol-bio\/"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/05\/exosome-gene-expression-ts-r-25018_eyecatch-1.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/05\/exosome-gene-expression-ts-r-25018_eyecatch-1.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/05\/exosome-gene-expression-ts-r-25018_eyecatch-1.jpg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/05\/exosome-gene-expression-ts-r-25018_eyecatch-1.jpg?resize=700%2C400&ssl=1 2x"},"classes":[]},{"id":25076,"url":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/experiment-qpcr-gsd-ts-tr-26015\/","url_meta":{"origin":201,"position":2},"title":"\u3010\u3084\u3063\u3066\u307f\u305f\u3011cDNA\u3092\u300c\u6df7\u305c\u308bvs.\u6df7\u305c\u306a\u3044\u300d\u3067Ct\u5024\u306f\u3069\u3046\u5909\u308f\u308b\uff1f","author":"LATB Staff","date":"2026\u5e747\u670827\u65e5","format":false,"excerpt":"\u2015 \u5b9f\u9a13\u73fe\u5834\u3067\u3088\u304f\u3042\u308b\"\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u5f8c\u5165\u308c\"\u64cd\u4f5c\u306e\u518d\u73fe\u6027\u3092\u5fb9\u5e95\u691c\u8a3c \u2015 \u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\uff08qPCR\u2026","rel":"","context":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","block_context":{"text":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","link":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/mol-bio\/"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/03\/experiment-qpcr-gsd-ts-tr-26015_eyecatch-scaled.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/03\/experiment-qpcr-gsd-ts-tr-26015_eyecatch-scaled.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/03\/experiment-qpcr-gsd-ts-tr-26015_eyecatch-scaled.jpg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/03\/experiment-qpcr-gsd-ts-tr-26015_eyecatch-scaled.jpg?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/03\/experiment-qpcr-gsd-ts-tr-26015_eyecatch-scaled.jpg?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2026\/03\/experiment-qpcr-gsd-ts-tr-26015_eyecatch-scaled.jpg?resize=1400%2C800&ssl=1 4x"},"classes":[]},{"id":24545,"url":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/digital-pcr-gene-expression-gsd-ts-sd-25082\/","url_meta":{"origin":201,"position":3},"title":"\u3010Absolute Q\u30c7\u30b8\u30bf\u30ebPCR\u30b7\u30b9\u30c6\u30e0\u306e\u6d3b\u7528\u4f8b\u3011\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2DNA\u306e\u5b9a\u91cf","author":"LatB Staff","date":"2026\u5e741\u670816\u65e5","format":false,"excerpt":"Applied Biosystems\u2122 QuantStudio\u2122 Absolute Q\u2122 \u30c7\u30b8\u30bf\u30ebP\u2026","rel":"","context":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","block_context":{"text":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","link":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/mol-bio\/"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/12\/digital-pcr-gene-expression-gsd-ts-sd-25082_eyecatch.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/12\/digital-pcr-gene-expression-gsd-ts-sd-25082_eyecatch.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/12\/digital-pcr-gene-expression-gsd-ts-sd-25082_eyecatch.jpg?resize=525%2C300&ssl=1 1.5x"},"classes":[]},{"id":23665,"url":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/pcr-dna-gsd-ts-tr-25064\/","url_meta":{"origin":201,"position":4},"title":"\u3010\u3084\u3063\u3066\u307f\u305f\u3011PCR\u306e\u30a2\u30cb\u30fc\u30ea\u30f3\u30b0\u6e29\u5ea6\u3092\u601d\u3044\u5207\u308a\u632f\u3063\u3066\u307f\u305f","author":"LatB Staff","date":"2025\u5e7410\u670828\u65e5","format":false,"excerpt":"\u65b0\u305f\u306aPCR\u691c\u51fa\u7cfb\u3092\u69cb\u7bc9\u3059\u308b\u5834\u5408\u3001\u4f55\u3088\u308a\u3082\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u914d\u5217\u304c\u91cd\u8981\u3068\u3044\u3046\u3053\u3068\u306f\u591a\u304f\u306e\u65b9\u304c\u8a8d\u8b58\u3057\u3066\u3044\u308b\u306e\u2026","rel":"","context":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","block_context":{"text":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","link":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/mol-bio\/"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/10\/pcr-dna-gsd-ts-tr-25064_eyecatch-scaled.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/10\/pcr-dna-gsd-ts-tr-25064_eyecatch-scaled.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/10\/pcr-dna-gsd-ts-tr-25064_eyecatch-scaled.jpg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/10\/pcr-dna-gsd-ts-tr-25064_eyecatch-scaled.jpg?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/10\/pcr-dna-gsd-ts-tr-25064_eyecatch-scaled.jpg?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/10\/pcr-dna-gsd-ts-tr-25064_eyecatch-scaled.jpg?resize=1400%2C800&ssl=1 4x"},"classes":[]},{"id":23052,"url":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/sanger-seq-ce-gsd-ts-ce-25058\/","url_meta":{"origin":201,"position":5},"title":"\u3010\u3084\u3063\u3066\u307f\u305f\u3011\u3000\u65b0\u3057\u3044\u30d9\u30fc\u30b9\u30b3\u30fc\u30e9\u30fc\u3092\u6d3b\u7528\u3057\u305f\u3001\u30c0\u30d6\u30ebIndel\u5909\u7570\u306e\u691c\u51fa","author":"LatB Staff","date":"2025\u5e749\u670825\u65e5","format":false,"excerpt":"\u30c0\u30a4\u30ec\u30af\u30c8\u30b7\u30fc\u30b1\u30f3\u30b9\u6cd5\u306f\u3001\u76ee\u7684\u306e\u5869\u57fa\u914d\u5217\u3092\u53d6\u5f97\u3059\u308b\u624b\u6cd5\u3068\u3057\u3066\u5e83\u304f\u5229\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u30d8\u30c6\u30ed\u63a5\u5408\u6027In\u2026","rel":"","context":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","block_context":{"text":"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023","link":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/mol-bio\/"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/09\/sanger-seq-ce-gsd-ts-ce-25058_eyecatch-scaled.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/09\/sanger-seq-ce-gsd-ts-ce-25058_eyecatch-scaled.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/09\/sanger-seq-ce-gsd-ts-ce-25058_eyecatch-scaled.jpg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/09\/sanger-seq-ce-gsd-ts-ce-25058_eyecatch-scaled.jpg?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/09\/sanger-seq-ce-gsd-ts-ce-25058_eyecatch-scaled.jpg?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/09\/sanger-seq-ce-gsd-ts-ce-25058_eyecatch-scaled.jpg?resize=1400%2C800&ssl=1 4x"},"classes":[]}],"jetpack_sharing_enabled":true,"amp_enabled":true,"jetpack_featured_media_url":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic-title-15.jpg","_links":{"self":[{"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/posts\/201","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/users\/988"}],"replies":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/comments?post=201"}],"version-history":[{"count":0,"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/posts\/201\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/media\/2321"}],"wp:attachment":[{"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/media?parent=201"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/categories?post=201"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/tags?post=201"},{"taxonomy":"division","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-json\/wp\/v2\/division?post=201"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}