{"id":204,"date":"2015-12-03T00:00:00","date_gmt":"2015-12-02T15:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/pcr-basic1\/"},"modified":"2024-08-27T11:36:42","modified_gmt":"2024-08-27T02:36:42","slug":"pcr-basic1","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/pcr-basic1\/","title":{"rendered":"PCR\u7523\u7269\u306b\u6c42\u3081\u308b\u3082\u306e\u306f\u4f55\u3067\u3059\u304b\uff1f PCR\u304c\u521d\u3081\u3066\u306e\u4eba\u306b\u6559\u3048\u3066\u3042\u3052\u305f\u3044\u3061\u3087\u3063\u3068\u3057\u305f\u30b3\u30c4\u305d\u306e1"},"content":{"rendered":"<p>PCR\u306f\u3001\u975e\u5e38\u306b\u7c21\u4fbf\u306a\u4f5c\u696d\u3067DNA\u65ad\u7247\u3092\u5927\u91cf\u306b\u5f97\u3089\u308c\u308b\u57fa\u790e\u6280\u8853\u3067\u3059\u3002\u305d\u3057\u3066PCR\u306e\u5b9f\u9a13\u306f\u3001\u673a\u4e0a\u3067\u30c7\u30b6\u30a4\u30f3\u3092\u3059\u308b\u3053\u3068\u306780\uff05\u4ee5\u4e0a\u306e\u6210\u529f\u3092\u5c0e\u3051\u308b\u6570\u5c11\u306a\u3044\u6280\u8853\u3067\u3059\u3002\u4eca\u56de\u306f\u3001PCR\u306e\u6280\u8853\u7684\u306a\u30dd\u30a4\u30f3\u30c8\u3084\u3001\u5931\u6557\u3057\u306a\u3044PCR\u30c7\u30b6\u30a4\u30f3\u306b\u3064\u3044\u3066\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n<h2>PCR\u7523\u7269\u306b\u6c42\u3081\u308b\u3082\u306e\u306f\u4f55\u3067\u3059\u304b\uff1f<\/h2>\n<h3>\u53ce\u91cf\u3068\u7279\u7570\u6027<\/h3>\n<p><strong>\u53ce\u91cf\u304c\u9ad8\u3044\u3068\u3044\u3046\u306e\u306f\u3001PCR\u52b9\u7387\u304c\u9ad8\u3044\u3068\u63db\u8a00\u3067\u304d\u307e\u3059\u3002<\/strong>\u305d\u3082\u305d\u3082PCR\u306f\u5fae\u91cf\u306e\u6838\u9178\u3092\u73fe\u5728\u306e\u5206\u6790\u6280\u8853\u3067\u89e3\u6790\u3067\u304d\u308b\u3088\u3046\u306b\u5897\u5e45\u3055\u305b\u308b\u6280\u8853\u3067\u3042\u308b\u305f\u3081\u3001\u305f\u304f\u3055\u3093\u306ePCR\u7523\u7269\u3092\u5f97\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u306e\u306f\u3001\u89e3\u6790\u306b\u304a\u3044\u3066\u5927\u304d\u306a\u30a2\u30c9\u30d0\u30f3\u30c6\u30fc\u30b8\u3067\u3059\u3002<br \/>\n\u3082\u3061\u308d\u3093\u9175\u7d20\u6d3b\u6027\u3092\u4e0a\u3052\u308c\u3070\u3001\u53ce\u91cf\u3082\u4e0a\u304c\u308b\u306e\u3067\u3059\u304c\u3001\u76ee\u7684\u306ePCR\u7523\u7269\u4ee5\u5916\u306e\u3082\u306e\u3082\u5897\u5e45\u3055\u308c\u3001\u7279\u7570\u6027\u304c\u4f4e\u4e0b\u3059\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002<br \/>\n\u3064\u307e\u308a\u3001\u9175\u7d20\u6d3b\u6027\u3092\u30d1\u30e9\u30e1\u30fc\u30bf\u306b\u8003\u3048\u308b\u3068\u3001<strong>\u53ce\u91cf\u3068\u7279\u7570\u6027\u306f\u8868\u88cf\u4e00\u4f53\u306e\u95a2\u4fc2\u306b\u3042\u308b<\/strong>\u3068\u3044\u3048\u307e\u3059\u3002<\/p>\n<h3>\u9396\u9577\u306e\u9577\u3055<\/h3>\n<p>90\u5e74\u4ee3\u4e2d\u76e4\u304b\u3089\u3001\u9577\u9396\u306ePCR\u7523\u7269\u3092\u5f97\u308b\u3053\u3068\u304c\u3067\u304d\u308bLong PCR\u3068\u3044\u3046\u6280\u8853\u304c\u666e\u53ca\u3057\u59cb\u3081\u307e\u3057\u305f\u3002\u3053\u306e\u9769\u65b0\u7684\u6280\u8853\u306f<strong>2\u3064\u306e\u5927\u304d\u306a\u5de5\u592b<\/strong>\u304c\u306a\u3055\u308c\u305f\u7d50\u679c\u3067\u3059\u30021\u3064\u76ee\u306f<strong>PCR\u9175\u7d20\u30922\u7a2e\u985e\u6df7\u5408\u3055\u305b\u3066\u3001\u9175\u7d20\u306e\u5408\u6210\u9593\u9055\u3044\u3092\u4f4e\u6e1b\u3055\u305b\u305f\u3053\u3068<\/strong>\u3067\u3059\u30022\u3064\u76ee\u306f\u3001<strong>\u30d0\u30c3\u30d5\u30a1\u30fc\u306e\u958b\u767a<\/strong>\u3067\u3059\u30022\u7a2e\u985e\u306e\u9175\u7d20\u306b\u5bfe\u5fdc\u3055\u305b\u308b\u4ee5\u5916\u306b\u3001\u6e29\u5ea6\u304c\u4e0a\u4e0b\u3059\u308b\u30d0\u30c3\u30d5\u30a1\u30fc\u306f\u3001\u9178\u89e3\u96e2\u5b9a\u6570\u306b\u3088\u308a\u6e29\u5ea6\u306b\u3088\u3063\u3066pH\u304c\u5909\u308f\u308a\u307e\u3059\u3002\u9175\u7d20\u6d3b\u6027\u3068pH\u306e\u95a2\u4fc2\u306f\u7533\u3057\u3042\u3052\u308b\u307e\u3067\u3082\u3042\u308a\u307e\u305b\u3093\u304c\u3001\u305d\u308c\u4ee5\u5916\u306b\u6838\u9178\u306e\u30d7\u30ea\u30f3\u74b0\u304c\u958b\u88c2\u3059\u308b\u640d\u50b7\u304c\u8d77\u3053\u308a\u307e\u3059\u3002\u3053\u308c\u304c\u8d77\u3053\u308b\u3068\u9175\u7d20\u306f\u3082\u306f\u3084\u5869\u57fa\u3092\u8a8d\u8b58\u3067\u304d\u305a\u306b\u3001\u4f38\u9577\u53cd\u5fdc\u306f\u5b8c\u5168\u306b\u6b62\u307e\u308a\u307e\u3059\u3002Long PCR\u306e\u30d0\u30c3\u30d5\u30a1\u30fc\u306f\u3001\u3053\u306e\u3088\u3046\u306apH\u306e\u5909\u52d5\u3092\u6291\u3048\u308b\u3088\u3046\u306a\u5de5\u592b\u304c\u306a\u3055\u308c\u3066\u3044\u307e\u3059\u3002<br \/>\n\u3057\u304b\u3057\u3001PCR\u306e\u539f\u7406\u304b\u3089\u8003\u3048\u308c\u3070\u3001\u3053\u306e\u6280\u8853\u9769\u65b0\u306fPCR\u3092\u7406\u60f3\u7684\u306a\u6761\u4ef6\u306b\u8fd1\u4ed8\u3051\u305f\u3068\u8a00\u3048\u3001\u9175\u7d20\u306e\u8aad\u307f\u9593\u9055\u3044\u3068\u6838\u9178\u306e\u640d\u50b7\u304cPCR\u306e\u5897\u5e45\u9396\u9577\u3092\u5236\u9650\u3057\u3066\u3044\u305f\u3068\u8a00\u3044\u63db\u3048\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002 \u4e00\u822c\u306b<strong>PCR\u9175\u7d20\u3092\u6df7\u5408\u3057\u306a\u3044PCR\u8a66\u85ac\u306e\u5834\u5408\u306f\u3001PCR\u7523\u7269\u306e\u9396\u9577\u304c2kb\u7a0b\u5ea6\u307e\u3067\u3001\u305d\u308c\u4ee5\u4e0a\u306e\u9577\u3055\u3092\u6c42\u3081\u308b\u5834\u5408\u306f\u3001\u6df7\u5408\u9175\u7d20\u306ePCR\u8a66\u85ac\u306e\u9078\u629e\u304c\u671b\u307e\u3057\u3044<\/strong>\u3067\u3059\u3002<\/p>\n<h3>PCR\u7523\u7269\u306e\u6b63\u78ba\u3055\uff08\u30d5\u30a3\u30c7\u30ea\u30c6\u30a3\uff09<\/h3>\n<p>\u6b63\u78ba\u3055\u306f\u30d5\u30a3\u30c7\u30ea\u30c6\u30a3\u3068\u3044\u3046\u30d1\u30e9\u30e1\u30fc\u30bf\u3067\u8868\u73fe\u3055\u308c\u307e\u3059\u3002PCR\u9175\u7d20\u306f1\u5206\u9593\u306b2\uff5e4kb\u3092\u5408\u6210\u3057\u307e\u3059\u3002\u975e\u5e38\u306b\u9ad8\u901f\u306b\u5408\u6210\u3092\u304a\u3053\u306a\u3063\u3066\u3044\u308b\u3053\u3068\u304c\u5206\u304b\u308a\u307e\u3059\u3002\u3057\u304b\u3057\u305d\u306e\u53cd\u9762\u3001\u8aa4\u3063\u305f\u5869\u57fa\u3067\u5408\u6210\u3057\u3066\u3057\u307e\u3046\u3053\u3068\u3082\u3042\u308a\u307e\u3059\u3002<strong>\u6b63\u3057\u304f\u5408\u6210\u3055\u308c\u308b\u983b\u5ea6\u3092\u30d5\u30a3\u30c7\u30ea\u30c6\u30a3\u3068\u8a00\u3044\u307e\u3059\u3002<\/strong>\u3053\u306e\u5024\u306fPCR\u6761\u4ef6\u306b\u3088\u3063\u3066\u7570\u306a\u308b\u306e\u3067\u3001\u4e00\u6982\u306b\u8a00\u3048\u306a\u3044\u306e\u3067\u3059\u304c\u3001\u4e00\u822c\u7684\u306b\u306f\u3001<strong>\u5869\u3084\u6dfb\u52a0\u5264\u306a\u3069\u3067\u9175\u7d20\u6d3b\u6027\u3092\u4e0a\u3052\u305f\u308a\u3001\u57fa\u8cea\u3067\u3042\u308bdNTP\u6fc3\u5ea6\u3092\u9ad8\u304f\u3057\u305f\u308a\u3001\u4f38\u9577\u53cd\u5fdc\u306e\u6e29\u5ea6\u3092\u4e0a\u3052\u305f\u308a\u3059\u308b\u3068\u30d5\u30a3\u30c7\u30ea\u30c6\u30a3\u306f\u4e0b\u304c\u308a\u307e\u3059\u3002<\/strong>\u9006\u306b\u3044\u3048\u3070\u3001\u53ce\u91cf\u306f\u843d\u3061\u307e\u3059\u304c<strong>\u5869\u3084\u57fa\u8cea\u3092\u6700\u5c0f\u9650\u5ea6\u306b\u3059\u308b\u3053\u3068\u3067\u5408\u6210\u9593\u9055\u3044\u306e\u5c11\u306a\u3044PCR\u7523\u7269\u304c\u5f97\u3089\u308c\u307e\u3059\u3002<\/strong><\/p>\n<h3>\u53ce\u91cf\u3001\u6b63\u78ba\u3055\uff08\u30d5\u30a3\u30c7\u30ea\u30c6\u30a3\uff09\u305d\u3057\u3066\u7279\u7570\u6027<\/h3>\n<p>PCR\u306e\u6027\u80fd\u3068\u3057\u3066\u3001\u53ce\u91cf\u3068\u6b63\u78ba\u3055\uff08\u30d5\u30a3\u30c7\u30ea\u30c6\u30a3\uff09\u306f\u91cd\u8981\u3067\u3059\u304c\u3001\u3053\u308c\u307e\u3067\u306b\u3082\u8a18\u3057\u307e\u3057\u305f\u304c\u3001\u4e00\u822c\u306b\u306f<strong>\u53ce\u91cf\u3068\u30d5\u30a3\u30c7\u30ea\u30c6\u30a3\u30fb\u7279\u7570\u6027\u306f\u76f8\u53cd\u3059\u308b\u8981\u6c42<\/strong>\u3067\u3059\u3002\u3057\u304b\u3057<strong>\u53ce\u91cf\u3068\u30d5\u30a3\u30c7\u30ea\u30c6\u30a3\u3092\u4e21\u7acb\u3059\u308b\u89e3\u6c7a\u7b56\u304c\u3001Long PCR\u306e\u6280\u8853\u3067\u3042\u308b\u9175\u7d20\u306e\u30d6\u30ec\u30f3\u30c9<\/strong>\u3067\u3059\u3002\u30d6\u30ec\u30f3\u30c9\u9175\u7d20\u306f\u9ad8\u6027\u80fd\u3067\u3042\u308b\u305f\u3081\u306b\u6bd4\u8f03\u7684\u306b\u9ad8\u4fa1\u3067\u306f\u3042\u308a\u307e\u3059\u304c\u3001\u53ce\u91cf\u3068\u30d5\u30a3\u30c7\u30ea\u30c6\u30a3\u306e\u4e21\u7acb\u3092\u6c42\u3081\u308b\u5b9f\u9a13\u306b\u306f\u6700\u9069\u3067\u3059\u3002<br \/>\n\u3055\u3066\u3001\u30d5\u30a3\u30c7\u30ea\u30c6\u30a3\u3068\u7279\u7570\u6027\u306f\u4f3c\u3066\u7570\u306a\u308a\u307e\u3059\u3002\u30d5\u30a3\u30c7\u30ea\u30c6\u30a3\u306f\u3001\u5408\u6210\u306e\u6b63\u78ba\u3055\u3067\u3001\u7279\u7570\u6027\u306fPCR\u7523\u7269\u304c\u5358\u4e00\u306e\u3082\u306e\u3067\u3042\u308b\u304b\u3068\u3044\u3046\u3053\u3068\u3067\u3059\u3002\u53ce\u91cf\u3068\u7279\u7570\u6027\u306e\u95a2\u4fc2\u306f\u3001\u9175\u7d20\u81ea\u8eab\u306e\u6d3b\u6027\u304c\u5f71\u97ff\u3057\u307e\u3059\u304c\u3001\u305d\u308c\u4ee5\u4e0a\u306b\u95a2\u4fc2\u3059\u308b\u306e\u304c\u30d7\u30e9\u30a4\u30de\u30fc\u81ea\u8eab\u306e\u30c7\u30b6\u30a4\u30f3\u3067\u3059\u3002\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u30c7\u30b6\u30a4\u30f3\u306b\u95a2\u3057\u3066\u306f\u6b21\u56de\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n<h2>\u3053\u308c\u3060\u3051\u306f\u77e5\u3063\u3066\u304a\u304d\u305f\u3044PCR\u306e\u57fa\u790e\u77e5\u8b58\u3001\u30b3\u30b3\u306b\u3042\u308a\u307e\u3059\uff01<\/h2>\n<p>Invitrogen \u5206\u5b50\u751f\u7269\u5b66\u6559\u5ba4\u3067\u306f\u3001\u5206\u5b50\u751f\u7269\u5b66\u306e\u6559\u80b2\u306e\u30e1\u30a4\u30f3\u3068\u3057\u3066\u3001\u521d\u5fc3\u8005\u3060\u3051\u3067\u306a\u304f\u7d4c\u9a13\u306e\u3042\u308b\u5206\u5b50\u751f\u7269\u5b66\u8005\u306e\u305f\u3081\u306b\u3001\u8c4a\u5bcc\u3067\u4fe1\u983c\u3067\u304d\u308b\u6280\u8853\u7684\u306a\u5185\u5bb9\u3092\u3054\u63d0\u4f9b\u3057\u3066\u3044\u307e\u3059\u3002\u57fa\u790e\u77e5\u8b58\u3092\u5b66\u3076\u6642\u306b\u306f\u3044\u3064\u3067\u3082\u3001Invitrogen \u5206\u5b50\u751f\u7269\u5b66\u6559\u5ba4\u3092\u6d3b\u7528\u3057\u3066\u304f\u3060\u3055\u3044\uff01<br \/>\n<a class=\"btn btn-red\" href=\"http:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/cloning\/cloning-learning-center\/invitrogen-school-of-molecular-biology\/pcr-education.html?CID=bid_mol_pch_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pcr3_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">\u57fa\u790e\u77e5\u8b58\u3092\u5b66\u3076<\/a><\/p>\n<p>\u4ed6\u306b\u3082\u3001PCR\u306e\u57fa\u790e\u306e\u5fa9\u7fd2\u3084\u3001\u3088\u308a\u6df1\u3044\u77e5\u8b58\u306e\u7372\u5f97\u3092\u76ee\u7684\u3068\u3057\u305f\u7121\u6599PDF\u3082\u63d0\u4f9b\u3057\u3066\u304a\u308a\u307e\u3059\uff01<\/p>\n<p>\u25bc\u4e3b\u306a\u5185\u5bb9<\/p>\n<ul>\n<li>PCR\u306e\u57fa\u790e\u77e5\u8b58<\/li>\n<li>PCR\u306e\u30bb\u30c3\u30c8\u30a2\u30c3\u30d7\u3067\u8003\u616e\u3059\u3079\u304d6\u3064\u306e\u8981\u7d20<\/li>\n<li>PCR\u53cd\u5fdc\u6761\u4ef6\u306b\u304a\u3051\u308b6 \u3064\u306e\u6ce8\u610f\u70b9<\/li>\n<li>DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306e4\u3064\u306e\u4e3b\u306a\u7279\u6027<\/li>\n<li>\u4e00\u822c\u7684\u306a10\u306ePCR\u6cd5<\/li>\n<li>PCR\u3092\u7528\u3044\u305f7\u3064\u306e\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3<\/li>\n<li>PCR\u306b\u95a2\u3059\u308b\u30c8\u30e9\u30d6\u30eb\u30b7\u30e5\u30fc\u30c6\u30a3\u30f3\u30b0\u30ac\u30a4\u30c9<\/li>\n<li>\u7cbe\u88fd\u30b0\u30ec\u30fc\u30c9\u9078\u629e\u306e\u30ac\u30a4\u30c0\u30f3\u30b9<\/li>\n<\/ul>\n<p>\u304a\u6c17\u8efd\u306b\u3054\u5229\u7528\u304f\u3060\u3055\u3044\uff3e\uff3e<\/p>\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/mol-bio-school.html?CID=bid_mol_pch_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pcr3_Social_LAB\" target=\"_blank\" rel=\"noopener 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