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(3-aminopropyltriethoxysilane)\u3084\u30dd\u30ea\u30ea\u30b8\u30f3\u3067\u51e6\u7406\u3057\u305f\u30ac\u30e9\u30b9\u30b9\u30e9\u30a4\u30c9\u3084\u3001\u30bc\u30e9\u30c1\u30f3\u3084\u30a2\u30eb\u30d6\u30df\u30f3\u306a\u3069\u3092\u542b\u3080\u63a5\u7740\u5264\u3092\u30b3\u30fc\u30c8\u3057\u305f\u30b9\u30e9\u30a4\u30c9\u30ac\u30e9\u30b9\u4e0a\u306b\u30de\u30a6\u30f3\u30c8\u3057\u307e\u3059\u3002\u305d\u306e\u5f8c\u3001\u7d44\u7e54\u5207\u7247\u3092\u30aa\u30fc\u30d6\u30f3\u3084\u96fb\u5b50\u30ec\u30f3\u30b8\u3067\u4e7e\u71e5\u3057\u307e\u3059\u3002<\/p>\n<p>\u51cd\u7d50\u7d44\u7e54\u306e\u5834\u5408\u3001\u30af\u30e9\u30a4\u30aa\u30b9\u30bf\u30c3\u30c8\uff08\u30df\u30af\u30ed\u30c8\u30fc\u30e0\u304c\u30bb\u30c3\u30c8\u3055\u308c\u305f\u4f4e\u6e29\u88c5\u7f6e\uff09\u3067\u5207\u7247\u5316\u3057\u3066\u30ac\u30e9\u30b9\u30b9\u30e9\u30a4\u30c9\u306b\u30de\u30a6\u30f3\u30c8\u3057\u307e\u3059\u3002\u3053\u306e\u5207\u7247\u306f\u901a\u5e38\u5ba4\u6e29\u3067\u4e00\u6669\u653e\u7f6e\u3057\u3066\u4e7e\u71e5\u3055\u305b\u305f\u5f8c\u3001-20\u2103\u306e\u30a2\u30bb\u30c8\u30f3\u306b\u6d78\u3057\u3066\u56fa\u5b9a\u5316\u3057\u307e\u3059\uff08\u4e7e\u71e5\u30b9\u30c6\u30c3\u30d7\u306f\u7d44\u7e54\u3084\u6297\u539f\u306b\u5fdc\u3058\u3066\u7701\u7565\u3059\u308b\u3053\u3068\u3082\u3042\u308a\u307e\u3059\uff09\u3002<\/p>\n<h3>\u8131\u30d1\u30e9\u30d5\u30a3\u30f3\u3068\u6297\u539f\u8ce6\u6d3b\u5316<\/h3>\n<p><strong>\u6a19\u7684\u6297\u539f\u306b\u6297\u4f53\u304c\u30a2\u30af\u30bb\u30b9\u3067\u304d\u308b\u3088\u3046\u306b\u3001\u30db\u30eb\u30de\u30ea\u30f3\u56fa\u5b9a\u30d1\u30e9\u30d5\u30a3\u30f3\u5305\u57cb\u3092\u884c\u3063\u305f\u7d44\u7e54\u5207\u7247\u304b\u3089\u3001\u30d1\u30e9\u30d5\u30a3\u30f3\u3092\u9664\u53bb\uff08\u8131\u30d1\u30e9\u30d5\u30a3\u30f3\uff09\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002<\/strong>\u8131\u30d1\u30e9\u30d5\u30a3\u30f3\u306b\u306f\u3001\u30ad\u30b7\u30ec\u30f3\u3084\u63ee\u767a\u6027\u306e\u6709\u6a5f\u6eb6\u5264\u3092\u5229\u7528\u3057\u307e\u3059\u3002\u307e\u305f\u3001\u30db\u30eb\u30e0\u30a2\u30eb\u30c7\u30d2\u30c9\u56fa\u5b9a\u3092\u884c\u3063\u305f\u30b5\u30f3\u30d7\u30eb\u3067\u306f\u3001\u30e1\u30c1\u30ec\u30f3\u67b6\u6a4b\u304c\u751f\u3058\u308b\u305f\u3081\u3001\u6297\u539f\u30a8\u30d4\u30c8\u30fc\u30d7\u306e\u30de\u30b9\u30af\u3084\u7acb\u4f53\u969c\u5bb3\u306b\u3088\u308a\u3001\u6297\u4f53\u306e\u30a2\u30af\u30bb\u30b9\u304c\u963b\u5bb3\u3055\u308c\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u3001\u30db\u30eb\u30de\u30ea\u30f3\u56fa\u5b9a\u3092\u884c\u3063\u305f\u7d44\u7e54\u5207\u7247\u306f\u3001\u6297\u4f53\u304c\u6297\u539f\u30a8\u30d4\u30c8\u30fc\u30d7\u3078\u30a2\u30af\u30bb\u30b9\u3057\u3084\u3059\u304f\u306a\u308b\u3088\u3046\u306b\u3001\u71b1\u51e6\u7406\u3084\u9175\u7d20\u6d88\u5316\u3068\u3044\u3063\u305f\u6297\u539f\u8ce6\u6d3b\u5316\u51e6\u7406\u3092\u884c\u3044\u307e\u3059\u3002<\/p>\n<h3>\u975e\u7279\u7570\u7684\u7d50\u5408\u306e\u30d6\u30ed\u30c3\u30af<\/h3>\n<p>\u6297\u4f53\u306f\u7279\u7570\u6027\u304c\u9ad8\u3044\u3068\u306f\u3044\u3048\u3001\u6297\u539f\u3068\u306f\u5225\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u306a\u3069\u306b\u5f31\u304f\u7d50\u5408\u3057\u3066\u3057\u307e\u3046\u3053\u3068\u304c\u3042\u308a\u3001\u3053\u308c\u304c\u6a19\u7684\u6297\u539f\u306e\u691c\u51fa\u306b\u304a\u3051\u308b\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9\u3068\u306a\u3063\u3066\u3057\u307e\u3044\u307e\u3059\u3002\u305d\u3053\u3067\u3001<strong>\u6297\u4f53\u306e\u975e\u7279\u7570\u7684\u7d50\u5408\u306b\u3088\u308b\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9\u3092\u4f4e\u4e0b\u3055\u305b\u308b\u305f\u3081\u306b\u3001\u6297\u4f53\u3092\u53cd\u5fdc\u3055\u305b\u308b\u524d\u306b\u3001\u7d44\u7e54\u5207\u7247\u3092\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u5264\u3067\u51e6\u7406\u3057\u307e\u3059\u3002<\/strong>\u514d\u75ab\u7d44\u7e54\u5316\u5b66\u5b9f\u9a13\u3067\u306f\u3001\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u5264\u3068\u3057\u3066\u3001\u4e8c\u6b21\u6297\u4f53\u30db\u30b9\u30c8\u3068\u540c\u7a2e\u306e\u975e\u514d\u75ab\u8840\u6e05\uff08\u6b63\u5e38\u8840\u6e05\uff09\u304c\u4e00\u822c\u7684\u306b\u5229\u7528\u3055\u308c\u307e\u3059\u3002\u305d\u306e\u307b\u304b\u3001\u30ce\u30f3\u30d5\u30a1\u30c3\u30c8\u30df\u30eb\u30af\u3001BSA\u3001\u30bc\u30e9\u30c1\u30f3\u306a\u3069\u3092\u542b\u3080\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u30d0\u30c3\u30d5\u30a1\u30fc\u3082\u5229\u7528\u3055\u308c\u307e\u3059\u3002<\/p>\n<h2>\u30b5\u30f3\u30d7\u30eb\u306e\u514d\u75ab\u67d3\u8272<\/h2>\n<h3>\u6297\u4f53\u53cd\u5fdc<\/h3>\n<p><strong>\u4e00\u822c\u7684\u306b\u306f\u3001\u6a19\u7684\u6297\u539f\u3068\u7279\u7570\u7684\u306a\u4e00\u6b21\u6297\u4f53\u3092\u53cd\u5fdc\u3055\u305b\u305f\u5f8c\u3001\u4e00\u6b21\u6297\u4f53\u3068\u7d50\u5408\u3059\u308b\u6a19\u8b58\u4e8c\u6b21\u6297\u4f53\u3092\u53cd\u5fdc\u3055\u305b\u307e\u3059\u3002<\/strong>\u6297\u4f53\u3092\u53cd\u5fdc\u3055\u305b\u305f\u5f8c\u306f\u3001\u975e\u7279\u7570\u7684\u306b\u5f31\u304f\u7d50\u5408\u3057\u305f\u6297\u4f53\u3092\u9664\u53bb\u3059\u308b\u305f\u3081\u306b\u3001\u9069\u5f53\u306a\u30d0\u30c3\u30d5\u30a1\u30fc\u3092\u7528\u3044\u3066\u30ea\u30f3\u30b9\u3057\u307e\u3059\u3002\u86cd\u5149\u6297\u4f53\u6cd5\u306e\u5834\u5408\u3001\u86cd\u5149\u6a19\u8b58\u6297\u4f53\u3092\u7528\u3044\u307e\u3059\u3002\u9175\u7d20\u6297\u4f53\u6cd5\u306e\u5834\u5408\u3001\u9175\u7d20\u6a19\u8b58\u6297\u4f53\u3068\u9069\u5f53\u306a\u767a\u8272\u57fa\u8cea\u3092\u7528\u3044\u307e\u3059\u3002\u9175\u7d20\u306f\u4e00\u822c\u7684\u306bHRP\uff08\u897f\u6d0b\u308f\u3055\u3073\u30da\u30eb\u30aa\u30ad\u30b7\u30c0\u30fc\u30bc\uff09\u304b\u30a2\u30eb\u30ab\u30ea\u30d5\u30a9\u30b9\u30d5\u30a1\u30bf\u30fc\u30bc\u3092\u5229\u7528\u3057\u3001\u305d\u308c\u305e\u308cDAB\uff08HRP\u7528\u306e\u57fa\u8cea\uff09\u3084NBT\/BCIP\uff08\u30a2\u30eb\u30ab\u30ea\u30d5\u30a9\u30b9\u30d5\u30a1\u30bf\u30fc\u30bc\u7528\u306e\u57fa\u8cea\uff09\u3092\u5229\u7528\u3057\u307e\u3059\u3002\u767a\u8272\u57fa\u8cea\u306f\u9175\u7d20\u3068\u53cd\u5fdc\u3059\u308b\u3068\u4e0d\u6eb6\u6027\u306e\u767a\u8272\u6c88\u6bbf\u3092\u751f\u3058\u307e\u3059\u3002<\/p>\n<h3>\u30ab\u30a6\u30f3\u30bf\u30fc\u67d3\u8272<\/h3>\n<p><strong>\u6297\u4f53\u306b\u3088\u308b\u691c\u51fa\u3068\u306e\u5bfe\u6bd4\u3084\u7d30\u80de\u69cb\u9020\u306e\u53ef\u8996\u5316\u3092\u884c\u3046\u305f\u3081\u306b\u30ab\u30a6\u30f3\u30bf\u30fc\u67d3\u8272\u3092\u884c\u3044\u307e\u3059\u3002<\/strong>\u30ab\u30a6\u30f3\u30bf\u30fc\u67d3\u8272\u306f\u901a\u5e38\u3001\u6297\u4f53\u53cd\u5fdc\u5f8c\u306b\u884c\u3044\u307e\u3059\u3002<\/p>\n<table>\n<tbody>\n<tr>\n<td style=\"text-align: center\"><strong>\u8272\u7d20\u306e\u30bf\u30a4\u30d7<\/strong><\/td>\n<td style=\"text-align: center\"><strong>\u8272\u7d20<\/strong><\/td>\n<td style=\"text-align: center\"><strong>\u30bf\u30fc\u30b2\u30c3\u30c8<\/strong><\/td>\n<td style=\"text-align: center\"><strong>\u8272<\/strong><\/td>\n<\/tr>\n<tr>\n<td>\u767a\u8272<\/td>\n<td>Hematoxylin<\/td>\n<td>\u6838<\/td>\n<td>\u9752\uff5e\u7d2b<\/td>\n<\/tr>\n<tr>\n<td>\u767a\u8272<\/td>\n<td>Nuclear fast red (Kernechtrot)<\/td>\n<td>\u6838\u9178<\/td>\n<td>\u8d64<\/td>\n<\/tr>\n<tr>\n<td>\u767a\u8272<\/td>\n<td>Methyl green<\/td>\n<td>\u6838\u9178<\/td>\n<td>\u7dd1<\/td>\n<\/tr>\n<tr>\n<td>\u86cd\u5149<\/td>\n<td>Hoechst stain<\/td>\n<td>\u6838\u9178<\/td>\n<td>\u9752<\/td>\n<\/tr>\n<tr>\n<td>\u86cd\u5149<\/td>\n<td>4&#8242;, 6-diamidino-2-phenylindole (DAPI)<\/td>\n<td>\u6838\u9178<\/td>\n<td>\u9752<\/td>\n<\/tr>\n<tr>\n<td>\u86cd\u5149<\/td>\n<td>Propidium iodide<\/td>\n<td>\u6838\u9178<\/td>\n<td>\u8d64<\/td>\n<\/tr>\n<tr>\n<td>\u86cd\u5149<\/td>\n<td>Fluorophore-tagged phalloidin<\/td>\n<td>\u30a2\u30af\u30c1\u30f3\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8<\/td>\n<td>\uff08\u86cd\u5149\u8272\u7d20\u306b\u3088\u308b\uff09<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h2>\u30b5\u30f3\u30d7\u30eb\u306e\u53ef\u8996\u5316<\/h2>\n<p><strong>\u9175\u7d20\u6297\u4f53\u6cd5\u306e\u5834\u5408\u3001\u5149\u5b66\u9855\u5fae\u93e1\u3067\u89b3\u5bdf\u3057\u307e\u3059\u3002\u86cd\u5149\u6297\u4f53\u6cd5\u306e\u5834\u5408\u3001\u86cd\u5149\u9855\u5fae\u93e1\u3067\u89b3\u5bdf\u3057\u307e\u3059\u3002<\/strong>\u3088\u308a\u30b3\u30f3\u30c8\u30e9\u30b9\u30c8\u3084\u5206\u89e3\u80fd\u306e\u9ad8\u3044\u753b\u50cf\u3092\u5f97\u308b\u305f\u3081\u306b\u306f\u3001\u5171\u7126\u70b9\u9855\u5fae\u93e1\u3092\u5229\u7528\u3057\u307e\u3059\u3002\u307e\u305f\u3001\u5f0a\u793e<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/cell-analysis\/cellular-imaging\/hcs-hca.html?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa15_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">\u7d30\u80de\u30a4\u30e1\u30fc\u30b8\u89e3\u6790\u30b7\u30b9\u30c6\u30e0<\/a>\u3092\u7528\u3044\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u7279\u5b9a\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3084\u5358\u4e00\u306e\u7d30\u80de\u306b\u3068\u3069\u307e\u3089\u305a\u3001\u8907\u6570\u306e\u7d30\u80de\u306e\u4e00\u3064\u4e00\u3064\u306b\u3064\u3044\u3066\u81ea\u52d5\u7684\u306b\u30c7\u30fc\u30bf\u53d6\u5f97\u3057\u7d71\u8a08\u5b66\u7684\u306b\u5ba2\u89b3\u7684\u306a\u89e3\u6790\u3092\u884c\u3046\u30cf\u30a4\u30b3\u30f3\u30c6\u30f3\u30c8\u30a2\u30ca\u30ea\u30b7\u30b9\u3053\u3068\u3082\u51fa\u6765\u307e\u3059\u3002<br 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