{"id":20930,"date":"2025-05-21T14:29:59","date_gmt":"2025-05-21T05:29:59","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/?p=20930"},"modified":"2025-06-12T15:18:54","modified_gmt":"2025-06-12T06:18:54","slug":"protein-expression-microorganism-culture-bid-ts-mbr-25030","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-expression-microorganism-culture-bid-ts-mbr-25030\/","title":{"rendered":"\u3010\u3053\u3093\u306a\u88fd\u54c1\u3042\u3063\u305f\u306e\u304b\uff01\u3011MagicMedia <i>E. coli<\/i> Expression Medium\u3092\u7528\u3044\u305f\u7c21\u5358\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe"},"content":{"rendered":"<p>\u300c\u3053\u3093\u306a\u88fd\u54c1\u3042\u3063\u305f\u306e\u304b\uff01\u300d\u30b7\u30ea\u30fc\u30ba\u3067\u306f\u3001\u3042\u307e\u308a\u77e5\u3089\u308c\u3066\u3044\u306a\u3044\u3051\u3069\u305c\u3072\u4f7f\u3063\u3066\u3044\u305f\u3060\u304d\u305f\u3044\u3068\u3063\u3066\u304a\u304d\u306e\u88fd\u54c1\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002\u4eca\u56de\u3054\u7d39\u4ecb\u3059\u308b\u306e\u306fInvitrogen\u2122 MagicMedia\u2122 <i>E. coli<\/i> Expression Medium\uff08\u4ee5\u4e0b<br \/>\nMagicMedia Medium\uff09\u3068\u3044\u3046\u5927\u8178\u83cc\u767a\u73fe\u7528\u57f9\u5730\u3067\u3059\u3002\u5927\u8178\u83cc\u306e\u5897\u6b96\u306b\u3068\u3082\u306a\u3044\u81ea\u52d5\u7684\u306b\u767a\u73fe\u8a98\u5c0e\u304c\u304b\u304b\u308b\u305f\u3081\u9762\u5012\u306aOD\u6e2c\u5b9a\u3092\u7701\u7565\u3067\u304d\u3001\u304b\u3064\u6700\u9069\u306a\u57f9\u5730\u7d44\u6210\u306b\u3088\u308a\u9ad8\u3044\u767a\u73fe\u91cf\u304c\u671f\u5f85\u3067\u304d\u307e\u3059\u3002\u672c\u30d6\u30ed\u30b0\u3067\u306f\u3001MagicMedia Medium\u3092\u4f7f\u7528\u3057\u305f\u4ee5\u4e0b\u306e\u691c\u8a0e\u7d50\u679c\u306b\u3064\u3044\u3066\u3054\u5831\u544a\u3044\u305f\u3057\u307e\u3059\u3002<\/p>\n<ul>\n<li>\u691c\u8a0e1\uff1aLB\u57f9\u5730\uff08+IPTG\uff09\u3068\u6bd4\u8f03\u3057\u3066\u307f\u305f\uff085 mL\u300137 \u2103\uff09<\/li>\n<li>\u691c\u8a0e2\uff1a\u4f4e\u6e29\u3067\u306e\u767a\u73fe\u3092\u8a66\u3057\u3066\u307f\u305f\uff085 mL\u300120 \u2103\uff09<\/li>\n<li>\u691c\u8a0e3\uff1a\u30b9\u30b1\u30fc\u30eb\u30a2\u30c3\u30d7\u3057\u3066\u307f\u305f\uff08200 mL\u300120 \u2103\uff09<\/li>\n<\/ul>\n<h2>MagicMedia <i>E. coli<\/i> Expression Medium\u3068\u306f\uff1f<\/h2>\n<p>\u691c\u8a0e\u7d50\u679c\u3092\u3054\u5831\u544a\u3059\u308b\u524d\u306bMagicMedia Medium\u306b\u3064\u3044\u3066\u7c21\u5358\u306b\u3054\u8aac\u660e\u3044\u305f\u3057\u307e\u3059\u3002<\/p>\n<ul>\n<li>BL21\uff08DE3\uff09\u306a\u3069\u306e\u5927\u8178\u83cc\u3068T7\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u3092\u6301\u3064\u5927\u8178\u83cc\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u3092\u7d44\u307f\u5408\u308f\u305b\u3066\u3001IPTG\u3092\u6dfb\u52a0\u305b\u305a\u306b\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u3092\u884c\u3048\u308b\u3002\u305f\u3060\u3057\u3001DH5\u03b1\u3001TOP10\u306a\u3069\u306e\u5927\u8178\u83cc\u682a\u306b\u306f\u4f7f\u7528\u3067\u304d\u306a\u3044<\/li>\n<li>OD\u6e2c\u5b9a\u304c\u4e0d\u8981\u3067\u5c0f\u30b9\u30b1\u30fc\u30eb\u767a\u73fe\u306a\u3089\u524d\u57f9\u990a\u3082\u4e0d\u8981\uff08\u56f32)<\/li>\n<li>LB\u57f9\u5730\u3068\u6bd4\u8f03\u3057\u3066\u9ad8\u5bc6\u5ea6\u57f9\u990a\u304c\u53ef\u80fd\u306a\u306e\u3067\u9ad8\u767a\u73fe\u304c\u671f\u5f85\u3067\u304d\u308b<\/li>\n<li>\u305d\u306e\u307e\u307e\u4f7f\u3048\u308b\u6db2\u4f53\u30bf\u30a4\u30d7\u3068\u7c89\u672b\u30bf\u30a4\u30d7\u304c\u3042\u308b\uff08\u56f31\uff09<\/li>\n<li>\u8abf\u88fd\u304c\u7c21\u5358\uff08\u6db2\u4f53\u30bf\u30a4\u30d7\u306f2\u6db2\u3092\u6df7\u5408\u3001\u7c89\u672b\u30bf\u30a4\u30d7\u306f\u6c34\u306b\u6eb6\u89e3\u30fb\u30aa\u30fc\u30c8\u30af\u30ec\u30fc\u30d6\u5f8c\u6dfb\u52a0\u5264\u3092\u52a0\u3048\u308b\u3060\u3051\uff09<\/li>\n<\/ul>\n<div id=\"attachment_20932\" style=\"width: 650px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-20932\" class=\"size-full wp-image-20932\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/05\/57fdac973c9cf8f873d58402fda74f73.png\" alt=\"\u56f31. MagicMedia E. coli Expression Medium\u306e\u5199\u771f\u3002A\uff1a\u7c89\u672b\u30bf\u30a4\u30d7\u3001B\uff1a\u6db2\u4f53\u30bf\u30a4\u30d7\" width=\"640\" height=\"361\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/05\/57fdac973c9cf8f873d58402fda74f73.png 640w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/05\/57fdac973c9cf8f873d58402fda74f73-300x169.png 300w\" sizes=\"auto, (max-width: 640px) 100vw, 640px\" \/><p id=\"caption-attachment-20932\" class=\"wp-caption-text\">\u56f31. MagicMedia <i>E. coli<\/i> Expression Medium\u306e\u5199\u771f\u3002A\uff1a\u7c89\u672b\u30bf\u30a4\u30d7\u3001B\uff1a\u6db2\u4f53\u30bf\u30a4\u30d7<\/p><\/div>\n<div id=\"attachment_20933\" style=\"width: 650px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-20933\" class=\"size-full wp-image-20933\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/05\/03dc6e1929e3d7a5169229739176c0e4.png\" alt=\"\u56f32. \u30d7\u30ed\u30c8\u30b3\u30eb\u306e\u6bd4\u8f03\" width=\"640\" height=\"519\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/05\/03dc6e1929e3d7a5169229739176c0e4.png 640w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/05\/03dc6e1929e3d7a5169229739176c0e4-300x243.png 300w\" sizes=\"auto, (max-width: 640px) 100vw, 640px\" \/><p id=\"caption-attachment-20933\" class=\"wp-caption-text\">\u56f32. \u30d7\u30ed\u30c8\u30b3\u30eb\u306e\u6bd4\u8f03\u3002<\/p><\/div>\n<h2>\u6750\u6599\uff1a\u4f7f\u7528\u3057\u305f\u767a\u73fe\u30d9\u30af\u30bf\u30fc<\/h2>\n<ul>\n<li>pEXP14-GFP\uff08Invitrogen\u2122 Gateway\u2122 pDEST\u212214 Vector\u306bGFP\u907a\u4f1d\u5b50\u3092\u7d44\u307f\u8fbc\u3093\u3060\u3082\u306e\uff09<\/li>\n<li>pET300\/NT-GW\/Rac Kinase\uff08Invitrogen\u2122 Champion\u2122 pET300\/NT-DEST\u306bRac kinase\u907a\u4f1d\u5b50\u3092\u7d44\u307f\u8fbc\u3093\u3060\u3082\u306e\u3001\u88fd\u54c1\u756a\u53f7K630001\u306b\u6dfb\u4ed8\u3055\u308c\u3066\u3044\u308b\uff09<\/li>\n<\/ul>\n<p>\u305d\u306e\u4ed6\u6750\u6599\u306b\u3064\u3044\u3066\u306f\u672c\u30d6\u30ed\u30b0\u306e\u6700\u5f8c\u306b\u307e\u3068\u3081\u3066\u8a18\u8f09\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<div id=\"attachment_20934\" style=\"width: 770px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-20934\" class=\"size-large wp-image-20934\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/05\/c39795dddde4fe1c98a6cf92dbc0d770-1024x394.png\" alt=\"\u56f33. \u4f7f\u7528\u3057\u305f\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u306e\u30de\u30c3\u30d7\" width=\"760\" height=\"292\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/05\/c39795dddde4fe1c98a6cf92dbc0d770-1024x394.png 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/05\/c39795dddde4fe1c98a6cf92dbc0d770-300x116.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/05\/c39795dddde4fe1c98a6cf92dbc0d770-768x296.png 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/05\/c39795dddde4fe1c98a6cf92dbc0d770.png 1387w\" sizes=\"auto, (max-width: 760px) 100vw, 760px\" \/><p id=\"caption-attachment-20934\" class=\"wp-caption-text\">\u56f33. \u4f7f\u7528\u3057\u305f\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u306e\u30de\u30c3\u30d7\uff08\u30de\u30c3\u30d7\u306e\u8aad\u307f\u65b9\u306b\u3064\u3044\u3066\u306f\u65e2\u5b58\u30d6\u30ed\u30b0\u3092\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\uff09\u3002<\/p><\/div>\n<h2>\u691c\u8a0e1\uff1aLB\u57f9\u5730\uff08+IPTG\uff09\u3068\u6bd4\u8f03\u3057\u3066\u307f\u305f\uff085 mL\u300137 \u2103\uff09<\/h2>\n<h3>\u65b9\u6cd5<\/h3>\n<ul>\n<li>LB\u57f9\u5730\uff1aLB\u57f9\u57305 mL\uff0850 mL\u5bb9\u30c1\u30e5\u30fc\u30d6\uff09\u306b\u524d\u57f9\u990a\u6db250 \u00b5L\u3092\u6dfb\u52a0\u219237 \u2103\u3067\u632f\u3068\u3046\u57f9\u990a\u958b\u59cb\u2192\u6642\u3005OD\uff08600 nm\uff09\u3092\u6e2c\u5b9a\u2192OD\uff08600 nm\uff09\u304c0.8\u7a0b\u5ea6\u306b\u306a\u3063\u305f\u30bf\u30a4\u30df\u30f3\u30b0\u3067IPTG\u6dfb\u52a0\uff08\u7d42\u6fc3\u5ea61 mM\uff09<\/li>\n<li>MagicMedia Medium\uff1a MagicMedia Medium 5 mL\uff0850 mL\u5bb9\u30c1\u30e5\u30fc\u30d6\uff09\u306b\u690d\u83cc\uff08\u30b3\u30ed\u30cb\u30fc\u304b\u3089\uff09\u219237 \u2103\u3067\u632f\u3068\u3046\u57f9\u990a\u958b\u59cb<\/li>\n<li>\u5171\u306b\u7d4c\u6642\u7684\u306b\u30b5\u30f3\u30d7\u30ea\u30f3\u30b0\u2192OD\uff08600 nm\uff09\u6e2c\u5b9a\u304a\u3088\u3073SDS-PAGE\uff08CBB\u67d3\u8272\uff09\u3067\u89e3\u6790<\/li>\n<\/ul>\n<div id=\"attachment_20935\" style=\"width: 929px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-20935\" class=\"size-full wp-image-20935\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/05\/1cceeadb37944a6d5337692806a37d9c.png\" alt=\"\u56f34. 600 nm\u306e\u5438\u5149\u5ea6\u3092\u5143\u306b\u4f5c\u6210\u3057\u305f\u5897\u6b96\u66f2\u7dda\u3002\u57f9\u990a\u6642\u9593\uff1a\u5171\u306b\u57f9\u990a\u958b\u59cb\u304b\u3089\u306e\u7d4c\u904e\u6642\u9593\u3002LB\u57f9\u5730\u306bIPTG\u3092\u6dfb\u52a0\u3057\u305f\u30bf\u30a4\u30df\u30f3\u30b0\u3092\u77e2\u5370\u3067\u793a\u3057\u305f\u3002\u691c\u8a0e2\uff08MagicMedia Medium\u300120 \u2103\uff09\u306e\u7d50\u679c\u3082\u542b\u3080\u3002\" width=\"919\" height=\"461\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/05\/1cceeadb37944a6d5337692806a37d9c.png 919w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/05\/1cceeadb37944a6d5337692806a37d9c-300x150.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/05\/1cceeadb37944a6d5337692806a37d9c-768x385.png 768w\" sizes=\"auto, (max-width: 919px) 100vw, 919px\" \/><p id=\"caption-attachment-20935\" class=\"wp-caption-text\">\u56f34. 600 nm\u306e\u5438\u5149\u5ea6\u3092\u5143\u306b\u4f5c\u6210\u3057\u305f\u5897\u6b96\u66f2\u7dda\u3002\u57f9\u990a\u6642\u9593\uff1a\u5171\u306b\u57f9\u990a\u958b\u59cb\u304b\u3089\u306e\u7d4c\u904e\u6642\u9593\u3002LB\u57f9\u5730\u306bIPTG\u3092\u6dfb\u52a0\u3057\u305f\u30bf\u30a4\u30df\u30f3\u30b0\u3092\u77e2\u5370\u3067\u793a\u3057\u305f\u3002\u691c\u8a0e2\uff08MagicMedia Medium\u300120 \u2103\uff09\u306e\u7d50\u679c\u3082\u542b\u3080\u3002<\/p><\/div>\n<div id=\"attachment_20936\" style=\"width: 747px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-20936\" class=\"size-full wp-image-20936\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/05\/eef60842b9bb8a724f4f3a7074a91c96.png\" alt=\"\u56f35. SDS-PAGE\u306e\u7d50\u679c\u3002A: \u57f9\u990a\u6db2\u54045 \u00b5L\u3092SDS\u5316\u3057\u305f\u3082\u306e\u3092\u30a2\u30d7\u30e9\u30a4\u3057\u305f\u3002B\uff1aOD\u6e2c\u5b9a\u7d50\u679c\u3092\u6307\u6a19\u306b\u83cc\u4f53\u6570\u3092\u305d\u308d\u3048\u305f\u30b5\u30f3\u30d7\u30eb\u3092\u30a2\u30d7\u30e9\u30a4\u3057\u305f\u3002\u57f9\u990a\u6642\u9593\uff1aLB\u57f9\u5730\u306e\u7d50\u679c\u306fIPTG\u6dfb\u52a0\u5f8c\u306e\u7d4c\u904e\u6642\u9593\u3002MagicMedia Medium\u306e\u7d50\u679c\u306f\u57f9\u990a\u958b\u59cb\u5f8c\u306e\u7d4c\u904e\u6642\u9593\u3002M: Invitrogen\u2122 Novex\u2122 Sharp Unstained Protein Standard\u3002\" width=\"737\" height=\"352\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/05\/eef60842b9bb8a724f4f3a7074a91c96.png 737w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/05\/eef60842b9bb8a724f4f3a7074a91c96-300x143.png 300w\" sizes=\"auto, (max-width: 737px) 100vw, 737px\" \/><p id=\"caption-attachment-20936\" class=\"wp-caption-text\">\u56f35. SDS-PAGE\u306e\u7d50\u679c\u3002A: \u57f9\u990a\u6db2\u54045 \u00b5L\u3092SDS\u5316\u3057\u305f\u3082\u306e\u3092\u30a2\u30d7\u30e9\u30a4\u3057\u305f\u3002B\uff1aOD\u6e2c\u5b9a\u7d50\u679c\u3092\u6307\u6a19\u306b\u83cc\u4f53\u6570\u3092\u305d\u308d\u3048\u305f\u30b5\u30f3\u30d7\u30eb\u3092\u30a2\u30d7\u30e9\u30a4\u3057\u305f\u3002\u57f9\u990a\u6642\u9593\uff1aLB\u57f9\u5730\u306e\u7d50\u679c\u306fIPTG\u6dfb\u52a0\u5f8c\u306e\u7d4c\u904e\u6642\u9593\u3002MagicMedia Medium\u306e\u7d50\u679c\u306f\u57f9\u990a\u958b\u59cb\u5f8c\u306e\u7d4c\u904e\u6642\u9593\u3002M: Invitrogen\u2122 Novex\u2122 Sharp Unstained Protein Standard\u3002<\/p><\/div>\n<h3>\u7d50\u679c\u3068\u8003\u5bdf<\/h3>\n<p>\u5438\u5149\u5ea6\u6e2c\u5b9a\u306e\u7d50\u679c\u304b\u3089\u3001MagicMedia Medium\u306e\u65b9\u304cLB\u57f9\u5730\u3088\u308a\u30824\uff5e5\u500d\u9ad8\u5bc6\u5ea6\u57f9\u990a\u304c\u53ef\u80fd\u306a\u3053\u3068\u304c\u793a\u5506\u3055\u308c\u307e\u3057\u305f\uff08\u56f34\uff09\u3002\u307e\u305f\u3001SDS-PAGE\u306e\u7d50\u679c\u304b\u3089\u3001\u57f9\u990a\u6db2\u91cf\u3042\u305f\u308a\u306e\u767a\u73fe\u91cf\u306fMagicMedia Medium\u306e\u65b9\u304c\u9ad8\u304f\uff08\u56f35A\uff09\u3001\u83cc\u4f53\u3042\u305f\u308a\u306e\u767a\u73fe\u91cf\u306f\u540c\u7a0b\u5ea6\u3067\u3042\u308b\u3053\u3068\u304c\u793a\u5506\u3055\u308c\u307e\u3057\u305f\uff08\u56f35B\uff09\u3002<\/p>\n<h2>\u691c\u8a0e2\uff1a\u4f4e\u6e29\u3067\u306e\u767a\u73fe\u3092\u8a66\u3057\u3066\u307f\u305f\uff085 mL\u300120 \u2103\uff09<\/h2>\n<h3>\u65b9\u6cd5<\/h3>\n<ul>\n<li>MagicMedia Medium 5 mL\uff0850 mL\u5bb9\u30c1\u30e5\u30fc\u30d6\uff09\u306b\u690d\u83cc\uff08\u30b3\u30ed\u30cb\u30fc\u304b\u3089\uff09\u219220 \u2103\u3067\u632f\u3068\u3046\u57f9\u990a\u958b\u59cb<\/li>\n<li>\u7d4c\u6642\u7684\u306b\u30b5\u30f3\u30d7\u30ea\u30f3\u30b0\u2192OD\uff08600 nm\uff09\u6e2c\u5b9a\u304a\u3088\u3073SDS-PAGE\uff08CBB\u67d3\u8272\uff09\u3067\u89e3\u6790<\/li>\n<\/ul>\n<div id=\"attachment_20937\" style=\"width: 455px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-20937\" class=\"size-full wp-image-20937\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/05\/4631d1d1d048f93df7cd8d3277009571.png\" alt=\"\u56f36 SDS-PAGE\u306e\u7d50\u679c\u3002OD\u6e2c\u5b9a\u7d50\u679c\u3092\u6307\u6a19\u306b\u83cc\u4f53\u6570\u3092\u305d\u308d\u3048\u305f\u30b5\u30f3\u30d7\u30eb\u3092\u30a2\u30d7\u30e9\u30a4\u3057\u305f\u3002\u57f9\u990a\u6642\u9593\uff1a\u57f9\u990a\u958b\u59cb\u5f8c\u306e\u7d4c\u904e\u6642\u9593\u3002M: Novex Sharp Unstained Protein Standard\u3002\" width=\"445\" height=\"314\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/05\/4631d1d1d048f93df7cd8d3277009571.png 445w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/05\/4631d1d1d048f93df7cd8d3277009571-300x212.png 300w\" sizes=\"auto, (max-width: 445px) 100vw, 445px\" \/><p id=\"caption-attachment-20937\" class=\"wp-caption-text\">\u56f36 SDS-PAGE\u306e\u7d50\u679c\u3002OD\u6e2c\u5b9a\u7d50\u679c\u3092\u6307\u6a19\u306b\u83cc\u4f53\u6570\u3092\u305d\u308d\u3048\u305f\u30b5\u30f3\u30d7\u30eb\u3092\u30a2\u30d7\u30e9\u30a4\u3057\u305f\u3002\u57f9\u990a\u6642\u9593\uff1a\u57f9\u990a\u958b\u59cb\u5f8c\u306e\u7d4c\u904e\u6642\u9593\u3002M: Novex Sharp Unstained Protein Standard\u3002<\/p><\/div>\n<h3>\u7d50\u679c\u3068\u8003\u5bdf<\/h3>\n<p>37 \u2103\u3068\u6bd4\u8f03\u3057\u3066\u7acb\u3061\u4e0a\u304c\u308a\u306b\u6642\u9593\u304c\u304b\u304b\u308a\u307e\u3057\u305f\u304c\u3001\u6700\u7d42\u7684\u306b\u306f\uff08\u672c\u5b9f\u9a13\u3067\u306f40\u6642\u9593\u5f8c\u4ed8\u8fd137 \u2103\u3068\u540c\u7a0b\u5ea6\u306e\u7d30\u80de\u5bc6\u5ea6\u307e\u3067\u5897\u6b96\u3057\uff08\u56f34\uff09\u3001\u304b\u3064\u83cc\u4f53\u3042\u305f\u308a\u306e\u767a\u73fe\u91cf\u3082\u540c\u7a0b\u5ea6\u3067\u3057\u305f\uff08\u56f36\uff09\u3002\u4ee5\u4e0a\u304b\u3089\u4f4e\u6e29\u3067\u3082\u554f\u984c\u306a\u304f\u767a\u73fe\u3067\u304d\u308b\u3053\u3068\u304c\u308f\u304b\u308a\u307e\u3057\u305f\u3002\u306a\u304a\u3001\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u306eSDS-PAGE\u306b\u304a\u3044\u3066CBB\u67d3\u8272\u30ec\u30d9\u30eb\u3067\u767a\u73fe\u3092\u78ba\u8a8d\u3067\u304d\u305f\u306e\u306f\u57f9\u990a\u958b\u59cb\u5f8c24\u6642\u9593\u4ee5\u964d\u3067\u3057\u305f\u3002\u5897\u6b96\u66f2\u7dda\u3068\u898b\u6bd4\u3079\u308b\u3068\u3001\u5bfe\u6570\u5897\u6b96\u671f\u306b\u5165\u3063\u305f\u3042\u305f\u308a\u306b\u767a\u73fe\u304c\u8a98\u5c0e\u3055\u308c\u305f\u3053\u3068\u304c\u4e88\u6e2c\u3055\u308c\u307e\u3059\u3002<\/p>\n<h2>\u691c\u8a0e3\uff1a\u30b9\u30b1\u30fc\u30eb\u30a2\u30c3\u30d7\u3057\u3066\u307f\u305f\uff08200 mL\u300120 \u2103\uff09<\/h2>\n<h3>\u65b9\u6cd5<\/h3>\n<p>pEXP14-GFP\u3092\u4f7f\u3063\u3066\u691c\u8a0e\u3092\u884c\u3044\u307e\u3057\u305f\u3002<\/p>\n<ul>\n<li>\u524d\u57f9\u990a\u3092\u884c\u3046\u5834\u5408\uff1aMagicMedia Medium 200 mL\uff08500 mL\u5bb9\u30d5\u30e9\u30b9\u30b3\uff09\u306b\u524d\u57f9\u990a\u6db210 mL\u3092\u6dfb\u52a0\u219220 \u2103\u3067\u632f\u3068\u3046\u57f9\u990a\u958b\u59cb<\/li>\n<li>\u524d\u57f9\u990a\u3092\u884c\u308f\u306a\u3044\u5834\u5408\uff1a MagicMedia Medium 200 mL\uff08500 mL\u5bb9\u30d5\u30e9\u30b9\u30b3\uff09\u306b\u690d\u83cc\uff08\u30b3\u30ed\u30cb\u30fc\u304b\u3089\uff09\u219220 \u2103\u3067\u632f\u3068\u3046\u57f9\u990a\u958b\u59cb<\/li>\n<li>\u5171\u306b\u7d4c\u6642\u7684\u306b\u30b5\u30f3\u30d7\u30ea\u30f3\u30b0\u2192OD\uff08600 nm\uff09\u6e2c\u5b9a\u304a\u3088\u3073SDS-PAGE\uff08CBB\u67d3\u8272\uff09\u3067\u89e3\u6790<\/li>\n<\/ul>\n<div id=\"attachment_20938\" style=\"width: 975px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-20938\" class=\"size-full wp-image-20938\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/05\/fe12dcb1a8ba9c768a8854509d75fa17.png\" alt=\"\" width=\"965\" height=\"425\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/05\/fe12dcb1a8ba9c768a8854509d75fa17.png 965w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/05\/fe12dcb1a8ba9c768a8854509d75fa17-300x132.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/05\/fe12dcb1a8ba9c768a8854509d75fa17-768x338.png 768w\" sizes=\"auto, (max-width: 965px) 100vw, 965px\" \/><p id=\"caption-attachment-20938\" class=\"wp-caption-text\">\u56f37. A\uff1a600 nm\u306e\u5438\u5149\u5ea6\u3092\u5143\u306b\u4f5c\u6210\u3057\u305f\u5897\u6b96\u66f2\u7dda\u3002B\uff1aSDS-PAGE\u306e\u7d50\u679c\u3002OD\u6e2c\u5b9a\u7d50\u679c\u3092\u7528\u3044\u3066\u83cc\u4f53\u6570\u3092\u305d\u308d\u3048\u305f\u30b5\u30f3\u30d7\u30eb\u3092\u30a2\u30d7\u30e9\u30a4\u3057\u305f\u3002M: Novex Sharp Unstained Protein Standard\u3002<\/p><\/div>\n<h3>\u7d50\u679c\u3068\u8003\u5bdf<\/h3>\n<p>\u4eca\u56de\u306e\u57f9\u990a\u6761\u4ef6\u3067\u306f\u6700\u7d42\u7684\u306a\u83cc\u4f53\u5bc6\u5ea6\u306f\u5c11\u30b9\u30b1\u30fc\u30eb\u57f9\u990a\u3088\u308a\u3082\u4f4e\u304f\u306a\u308a\u307e\u3057\u305f\u304c\u3001\u83cc\u4f53\u6570\u3042\u305f\u308a\u306e\u767a\u73fe\u30bf\u30f3\u30d1\u30af\u8cea\u91cf\u306f\u540c\u7a0b\u5ea6\u3067\u3057\u305f\uff08\u56f37B\uff09\u3002200 mL\u306b\u30b9\u30b1\u30fc\u30eb\u30a2\u30c3\u30d7\u3057\u3066\u3082\u767a\u73fe\u3092\u884c\u3048\u308b\u3053\u3068\u306f\u308f\u304b\u308a\u307e\u3057\u305f\u304c\u3001\u6700\u9069\u306a\u767a\u73fe\u6761\u4ef6\u3092\u898b\u3064\u3051\u308b\u305f\u3081\u306b\u3001\u6db2\u91cf\u3001\u57f9\u990a\u5bb9\u5668\u3001\u632f\u3068\u3046\u901f\u5ea6\u3092\u691c\u8a0e\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u305d\u3046\u3067\u3059\u3002\u306a\u304a\u3001\u30b3\u30ed\u30cb\u30fc\u304b\u3089\u57f9\u990a\u3092\u958b\u59cb\u3057\u305f\u5834\u5408\u3001\u5bfe\u6570\u5897\u6b96\u671f\u306b\u5165\u308b\u307e\u3067\u306b\u4e00\u65e5\u7a0b\u5ea6\u304b\u304b\u308a\u3001\u304b\u3064\u30d7\u30e9\u30c8\u30fc\u306b\u306a\u308b\u83cc\u4f53\u6fc3\u5ea6\u304c\u4f4e\u304f\u306a\u308a\u305d\u3046\u3067\u3059\u3002\u30b3\u30ed\u30cb\u30fc\u304b\u3089\u3067\u3082\u767a\u73fe\u306f\u884c\u3048\u307e\u3059\u304c\u3001\u30b9\u30b1\u30fc\u30eb\u30a2\u30c3\u30d7\u3092\u884c\u3063\u305f\u5834\u5408\u306f\u524d\u57f9\u990a\u3092\u884c\u3063\u305f\u65b9\u304c\u3088\u3055\u305d\u3046\u3067\u3059\u3002<\/p>\n<h2>\u307e\u3068\u3081<\/h2>\n<p>2\u907a\u4f1d\u5b50\u306b\u95a2\u3057\u3066\u3067\u3059\u304c\u3001\u4ee5\u4e0b\u306e\u3053\u3068\u3092\u78ba\u8a8d\u3067\u304d\u307e\u3057\u305f\u3002<\/p>\n<ul>\n<li>MagicMedia Medium\u3092\u7528\u3044\u3066LB\u57f9\u5730\uff0bIPTG\u3068\u540c\u7b49\u306b\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u3092\u884c\u3048\u308b<\/li>\n<li>\u7d30\u80de\u3042\u305f\u308a\u306e\u767a\u73fe\u30ec\u30d9\u30eb\u306fLB\u57f9\u5730\uff0bIPTG\u3068\u540c\u7a0b\u5ea6<\/li>\n<li>\u9ad8\u5bc6\u5ea6\u57f9\u990a\u304c\u53ef\u80fd\u306a\u305f\u3081\u3001\u57f9\u5730\u91cf\u3042\u305f\u308a\u306e\u767a\u73fe\u91cf\u306fLB\u57f9\u5730\uff0bIPTG\u3088\u308a\u3082\u9ad8\u3044<\/li>\n<li>MagicMedia Medium\u3092\u7528\u3044\u308b\u5834\u5408\u306fIPTG\u6dfb\u52a0\u304c\u4e0d\u8981\u306a\u305f\u3081\u5438\u5149\u5ea6\u6e2c\u5b9a\u304c\u4e0d\u8981\u3067\u5927\u5909\u697d<\/li>\n<li>\u5c11\u306a\u304f\u3068\u3082\u5c0f\u30b9\u30b1\u30fc\u30eb\u57f9\u990a\u3067\u3042\u308c\u3070\u524d\u57f9\u990a\u3082\u4e0d\u8981<\/li>\n<li>\u4f4e\u6e29\u3067\u3082\u905c\u8272\u306a\u304f\u767a\u73fe\u3092\u884c\u3048\u305f<\/li>\n<li>200 mL\u306b\u30b9\u30b1\u30fc\u30eb\u30a2\u30c3\u30d7\u3057\u3066\u3082\u767a\u73fe\u3092\u884c\u3048\u305f<\/li>\n<\/ul>\n<p>MagicMedia Medium\u306f\u3001LB\u57f9\u5730\uff0bIPTG\u3088\u308a\u3082\u304b\u306a\u308a\u64cd\u4f5c\u304c\u697d\u306a\u3053\u3068\u304c\u308f\u304b\u308a\u307e\u3057\u305f\u3002\u7279\u306b\u30b5\u30f3\u30d7\u30eb\u6570\u304c\u591a\u3044\u5834\u5408\u306b\u529b\u3092\u767a\u63ee\u3057\u305d\u3046\u3067\u3059\u3002\u661496\u30a6\u30a7\u30eb\u30c7\u30a3\u30fc\u30d7\u30a6\u30a7\u30eb\u30d7\u30ec\u30fc\u30c8\u3067\u8907\u6570\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u89e3\u6790\u3092\u884c\u3063\u3066\u3044\u307e\u3057\u305f\u304c\u3001\u305d\u306e\u6642\u306bMagicMedia Medium\u304c\u3042\u308c\u3070\u3088\u304b\u3063\u305f\u3068\u601d\u3044\u307e\u3057\u305f\u3002\u540c\u3058\u3088\u3046\u306a\u60a9\u307f\u3092\u304a\u6301\u3061\u306e\u65b9\u306f\u305c\u3072\u8a66\u3057\u3066\u307f\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n<p>\u307e\u305f\u5b9f\u969b\u304a\u8a66\u3057\u3044\u305f\u3060\u304f\u969b\u306b\u3001\u3053\u306e\u30d9\u30af\u30bf\u30fc\u306f\u4f7f\u3048\u308b\u306e\u304b\uff1f\u3053\u306e\u5927\u8178\u83cc\u3067\u3082\u5927\u4e08\u592b\u306a\u306e\u304b\uff1f\u306a\u3069\u3001\u3054\u4e0d\u660e\u306a\u70b9\u306a\u3069\u3042\u308a\u307e\u3057\u305f\u3089\u305c\u3072\u30c6\u30af\u30cb\u30ab\u30eb\u30b5\u30dd\u30fc\u30c8\uff08<a href=\"mailto:jptech@thermofisher.com?CID=ts_mol_r04_jp_cp0000_pjt0000_ts00000_0so_blg_protein_expression_microorganism_culture_ts_ts_mbr_25030_Social_LAB\" target=\"_blank\" rel=\"noopener\">jptech@thermofisher.com<\/a>\uff09\u306b\u304a\u554f\u3044\u5408\u308f\u305b\u304f\u3060\u3055\u3044\u3002<\/p>\n<h3>\u4f7f\u7528\u3057\u305f\u88fd\u54c1\u307e\u3068\u3081<\/h3>\n<table style=\"border-collapse: collapse;border: 1px solid black;width: 847px;height: 560px\" border=\"1\" cellspacing=\"0\">\n<tbody>\n<tr style=\"background-color: #f0f0f0\">\n<td style=\"padding: 10px;border: 1px solid black;text-align: left;width: 70px\"><b>\u00a0<\/b><\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left;width: 303px\"><b>\u88fd\u54c1\u540d<\/b><\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left;width: 100px\"><b>\u30ab\u30bf\u30ed\u30b0\u756a\u53f7<\/b><\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left;width: 89px\"><b>\u30b5\u30a4\u30ba<\/b><\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left;width: 147px\"><b>\u5099\u8003<\/b><\/td>\n<\/tr>\n<tr>\n<td style=\"padding: 10px;border: 1px solid black;text-align: center;vertical-align: middle;width: 70px\" rowspan=\"3\">\u4f7f\u7528<\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left\">Invitrogen\u2122 MagicMedia\u2122 <i>E. coli<\/i> Expression Medium, Powder<\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left;width: 100px\"><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/K6810?CID=ts_mol_r04_jp_cp0000_pjt0000_ts00000_0so_blg_protein_expression_microorganism_culture_ts_ts_mbr_25030_Social_LAB\" target=\"_blank\" rel=\"noopener\">K6810<\/a><\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left\">1 L<\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left\">\u7c89\u672b<\/td>\n<\/tr>\n<tr>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left\">Invitrogen\u2122 MagicMedia\u2122 <i>E. coli<\/i> Expression Medium, Powder<\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left;width: 100px\"><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/K6815?CID=ts_mol_r04_jp_cp0000_pjt0000_ts00000_0so_blg_protein_expression_microorganism_culture_ts_ts_mbr_25030_Social_LAB\" target=\"_blank\" rel=\"noopener\">K6815<\/a><\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left\">5 X 1 L<\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left\">\u7c89\u672b<\/td>\n<\/tr>\n<tr>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left\">Invitrogen\u2122 MagicMedia\u2122 <i>E. coli<\/i> Expression Medium<\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left;width: 100px\"><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/K6803?CID=ts_mol_r04_jp_cp0000_pjt0000_ts00000_0so_blg_protein_expression_microorganism_culture_ts_ts_mbr_25030_Social_LAB\" target=\"_blank\" rel=\"noopener\">K6803<\/a><\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left\">1 L<\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left\">\u6db2\u4f53<\/td>\n<\/tr>\n<tr>\n<td style=\"padding: 10px;border: 1px solid black;text-align: center;width: 70px\">\u4f7f\u7528<\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left\">Invitrogen\u2122 Luria Broth Base (Miller&#8217;s LB Broth Base), powder<\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left;width: 100px\"><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/12795027?CID=ts_mol_r04_jp_cp0000_pjt0000_ts00000_0so_blg_protein_expression_microorganism_culture_ts_ts_mbr_25030_Social_LAB\" target=\"_blank\" rel=\"noopener\">12795027<\/a><\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left\">500 g<\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left\"><\/td>\n<\/tr>\n<tr>\n<td style=\"padding: 10px;border: 1px solid black;text-align: center;width: 70px\">\u4f7f\u7528<\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left\">Carbenicillin Disodium Salt<\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left;width: 100px\"><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/10177012?CID=ts_mol_r04_jp_cp0000_pjt0000_ts00000_0so_blg_protein_expression_microorganism_culture_ts_ts_mbr_25030_Social_LAB\" target=\"_blank\" rel=\"noopener\">10177012<\/a><\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left\">5 g<\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left\"><\/td>\n<\/tr>\n<tr>\n<td style=\"padding: 10px;border: 1px solid black;text-align: center;width: 70px\">\u4f7f\u7528<\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left\">Invitrogen\u2122 IPTG<\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left;width: 100px\"><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/15529019?CID=ts_mol_r04_jp_cp0000_pjt0000_ts00000_0so_blg_protein_expression_microorganism_culture_ts_ts_mbr_25030_Social_LAB\" target=\"_blank\" rel=\"noopener\">15529019<\/a><\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left\">1 g<\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left\"><\/td>\n<\/tr>\n<tr>\n<td style=\"padding: 10px;border: 1px solid black;text-align: center;width: 70px\">\u4f7f\u7528<\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left\">Invitrogen\u2122 One Shot\u2122 BL21 Star\u2122 (DE3) Chemically Competent <i>E. coli<\/i><\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left;width: 100px\"><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/C601003?CID=ts_mol_r04_jp_cp0000_pjt0000_ts00000_0so_blg_protein_expression_microorganism_culture_ts_ts_mbr_25030_Social_LAB\" target=\"_blank\" rel=\"noopener\">C601003<\/a><\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left\">21\u53cd\u5fdc<\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left\"><\/td>\n<\/tr>\n<tr>\n<td style=\"padding: 10px;border: 1px solid black;text-align: center;width: 70px\">\u4f7f\u7528<\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left\">Invitrogen\u2122 Champion\u2122 pET300\/NT-DEST and pET301\/CT-DEST Gateway\u2122 Vector Kit<\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left;width: 100px\"><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/K630001?CID=ts_mol_r04_jp_cp0000_pjt0000_ts00000_0so_blg_protein_expression_microorganism_culture_ts_ts_mbr_25030_Social_LAB\" target=\"_blank\" rel=\"noopener\">K630001<\/a><\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left\"><\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left\">\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u30d9\u30af\u30bf\u30fc\u306e\u307f\u4f7f\u7528<\/td>\n<\/tr>\n<tr>\n<td style=\"padding: 10px;border: 1px solid black;text-align: center;width: 70px\">\u4f7f\u7528<\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left\">Invitrogen\u2122 Gateway\u2122 pDEST\u212214 Vector<\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left;width: 100px\"><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/11801016?CID=ts_mol_r04_jp_cp0000_pjt0000_ts00000_0so_blg_protein_expression_microorganism_culture_ts_ts_mbr_25030_Social_LAB\" target=\"_blank\" rel=\"noopener\">11801016<\/a><\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left\"><\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left\"><\/td>\n<\/tr>\n<tr>\n<td style=\"padding: 10px;border: 1px solid black;text-align: center;width: 70px\">\u4f7f\u7528<\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left\">XCell <i>SureLock<\/i> Mini-Cell<\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left;width: 100px\"><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/EI0001?CID=ts_mol_r04_jp_cp0000_pjt0000_ts00000_0so_blg_protein_expression_microorganism_culture_ts_ts_mbr_25030_Social_LAB\" target=\"_blank\" rel=\"noopener\">EI0001<\/a><\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left\"><\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left\"><\/td>\n<\/tr>\n<tr>\n<td style=\"padding: 10px;border: 1px solid black;text-align: center;width: 70px\">\u4f7f\u7528<\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left\">Invitrogen\u2122 NuPAGE\u2122 Bis-Tris Mini Protein Gels, 4\u201312%, 1.0\u20131.5 mm<\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left;width: 100px\"><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/NP0322BOX?CID=ts_mol_r04_jp_cp0000_pjt0000_ts00000_0so_blg_protein_expression_microorganism_culture_ts_ts_mbr_25030_Social_LAB\" target=\"_blank\" rel=\"noopener\">NP0322BOX<\/a><\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left\">10 gels<\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left\"><\/td>\n<\/tr>\n<tr>\n<td style=\"padding: 10px;border: 1px solid black;text-align: center;width: 70px\">\u4f7f\u7528<\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left\">Invitrogen\u2122 NuPAGE\u2122 MES SDS Running Buffer (20X)<\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left;width: 100px\"><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/NP0002?CID=ts_mol_r04_jp_cp0000_pjt0000_ts00000_0so_blg_protein_expression_microorganism_culture_ts_ts_mbr_25030_Social_LAB\" target=\"_blank\" rel=\"noopener\">NP0002<\/a><\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left\">500 mL<\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left\"><\/td>\n<\/tr>\n<tr>\n<td style=\"padding: 10px;border: 1px solid black;text-align: center;width: 70px\">\u4f7f\u7528<\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left\">Invitrogen\u2122 Novex\u2122 Sharp Unstained Protein Standard<\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left;width: 100px\"><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/LC5801?CID=ts_mol_r04_jp_cp0000_pjt0000_ts00000_0so_blg_protein_expression_microorganism_culture_ts_ts_mbr_25030_Social_LAB\" target=\"_blank\" rel=\"noopener\">LC5801<\/a><\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left\">2 \u00d7 250 \u00b5l<\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left\"><\/td>\n<\/tr>\n<tr>\n<td style=\"padding: 10px;border: 1px solid black;text-align: center;width: 70px\">\u4f7f\u7528<\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left\">Invitrogen\u2122 SimplyBlue\u2122 SafeStain<\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left;width: 100px\"><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/LC6060?CID=ts_mol_r04_jp_cp0000_pjt0000_ts00000_0so_blg_protein_expression_microorganism_culture_ts_ts_mbr_25030_Social_LAB\" target=\"_blank\" rel=\"noopener\">LC6060<\/a><\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left\">1 L<\/td>\n<td style=\"padding: 10px;border: 1px solid black;text-align: left\"><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h4>\u30c6\u30af\u30cb\u30ab\u30eb\u30bb\u30df\u30ca\u30fc\uff08\u7121\u6599\uff09<\/h4>\n<p>\u30d9\u30af\u30bf\u30fc\u30de\u30c3\u30d7\u306e\u8aad\u307f\u65b9\uff5e\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30fb\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u3067\u8ff7\u5b50\u306b\u306a\u3089\u306a\u3044\u305f\u3081\u306b<\/p>\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/technical-resources\/seminars\/vector-maps.html?CID=ts_mol_r04_jp_cp0000_pjt0000_ts00000_0so_blg_protein_expression_microorganism_culture_ts_ts_mbr_25030_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">\u304a\u7533\u8fbc\u307f\u306f\u3053\u3061\u3089<\/a><\/p>\n<h4>\u30cf\u30f3\u30ba\u30aa\u30f3\u30c8\u30ec\u30fc\u30cb\u30f3\u30b0\uff08\u6709\u511f\uff09<\/h4>\n<p>\u521d\u3081\u3066\u306e\u30af\u30ed\u30fc\u30cb\u30f3\u30b0 \u30cf\u30f3\u30ba\u30aa\u30f3\u30c8\u30ec\u30fc\u30cb\u30f3\u30b0<\/p>\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/technical-resources\/trainings\/cloning.html?CID=ts_mol_r04_jp_cp0000_pjt0000_ts00000_0so_blg_protein_expression_microorganism_culture_ts_ts_mbr_25030_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">\u304a\u7533\u8fbc\u307f\u306f\u3053\u3061\u3089<\/a><\/p>\n<h4>\u30d9\u30af\u30bf\u30fc\u30de\u30c3\u30d7\u306e\u8aad\u307f\u65b9\u30ac\u30a4\u30c9 \u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9<\/h4>\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/vector-map-guide.html?CID=ts_mol_r04_jp_cp0000_pjt0000_ts00000_0so_blg_protein_expression_microorganism_culture_ts_ts_mbr_25030_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u306f\u3053\u3061\u3089<\/a><\/p>\n<h4>Protein Expression\u30cf\u30f3\u30c9\u30d6\u30c3\u30af \u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9<\/h4>\n<p>\u30bf\u30f3\u30d1\u30af\u767a\u73fe\u306e\u5b9f\u9a13\u3092\u691c\u8a0e\u3057\u3066\u3044\u308b\u65b9\u3084\u3001\u8a73\u7d30\u60c5\u5831\u3092\u78ba\u8a8d\u3057\u305f\u3044\u65b9\u306b\u3046\u3063\u3066\u3064\u3051\u306e\u30cf\u30f3\u30c9\u30d6\u30c3\u30af\u3067\u3059\u3002<\/p>\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/protein-expression-handbook-download.html?CID=ts_mol_r04_jp_cp0000_pjt0000_ts00000_0so_blg_protein_expression_microorganism_culture_ts_ts_mbr_25030_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u306f\u3053\u3061\u3089<\/a><\/p>\n<h3>\u95a2\u9023\u3059\u308b\u30d6\u30ed\u30b0\u8a18\u4e8b<\/h3>\n<table style=\"border-color: lightgray;border-collapse: collapse;padding: 20px\" border=\"1\">\n<tbody>\n<tr>\n<td style=\"border-color: lightgray;padding: 20px\">\n<ul>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein_expression_mb_bid_ts_1\/?CID=ts_mol_r04_jp_cp0000_pjt0000_ts00000_0so_blg_protein_expression_microorganism_culture_ts_ts_mbr_25030_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3042\u306a\u305f\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u3068\u3063\u3066\u6700\u9069\u306a\u767a\u73fe\u7cfb\u306f\uff1f<\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/cloning-bid-ts-1\/?CID=ts_mol_r04_jp_cp0000_pjt0000_ts00000_0so_blg_protein_expression_microorganism_culture_ts_ts_mbr_25030_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30d9\u30af\u30bf\u30fc\u30de\u30c3\u30d7\u306e\u8aad\u307f\u65b9\uff5e\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30fb\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u3067\u8ff7\u5b50\u306b\u306a\u3089\u306a\u3044\u305f\u3081\u306b \u7b2c1\u56de \u30d7\u30e9\u30b9\u30df\u30c9\u30d9\u30af\u30bf\u30fc\u306e\u69cb\u9020\u3068\u30de\u30c3\u30d7\u304b\u3089\u5206\u304b\u308b\u3053\u3068<\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-expression-cloning-2-bid-ts-mbr-24076\/?CID=ts_mol_r04_jp_cp0000_pjt0000_ts00000_0so_blg_protein_expression_microorganism_culture_ts_ts_mbr_25030_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30d9\u30af\u30bf\u30fc\u30de\u30c3\u30d7\u306e\u8aad\u307f\u65b9\uff5e\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30fb\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u3067\u8ff7\u5b50\u306b\u306a\u3089\u306a\u3044\u305f\u3081\u306b \u7b2c2\u56de \u30d7\u30e9\u30b9\u30df\u30c9\u30d9\u30af\u30bf\u30fc\u306b\u542b\u307e\u308c\u308b\u30a8\u30ec\u30e1\u30f3\u30c8\u30ea\u30b9\u30c8<\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-expression-cloning-3-bid-ts-mbr-25003\/?CID=ts_mol_r04_jp_cp0000_pjt0000_ts00000_0so_blg_protein_expression_microorganism_culture_ts_ts_mbr_25030_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30d9\u30af\u30bf\u30fc\u30de\u30c3\u30d7\u306e\u8aad\u307f\u65b9\uff5e\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30fb\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u3067\u8ff7\u5b50\u306b\u306a\u3089\u306a\u3044\u305f\u3081\u306b \u7b2c3\u56de \u30d9\u30af\u30bf\u30fc\u30de\u30c3\u30d7\u306e\u8aad\u307f\u65b9\u30fb\u5b9f\u4f8b\u96c6\uff11<\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-expression-cloning-bid-ts-mbr-25035\/?CID=ts_mol_r04_jp_cp0000_pjt0000_ts00000_0so_blg_protein_expression_microorganism_culture_ts_ts_mbr_25030_Social_LAB\">\u30d9\u30af\u30bf\u30fc\u30de\u30c3\u30d7\u306e\u8aad\u307f\u65b9\uff5e\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30fb\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u3067\u8ff7\u5b50\u306b\u306a\u3089\u306a\u3044\u305f\u3081\u306b \u7b2c4\u56de 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