{"id":211,"date":"2015-12-10T00:00:00","date_gmt":"2015-12-09T15:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/pcr-basic2\/"},"modified":"2024-08-27T11:35:20","modified_gmt":"2024-08-27T02:35:20","slug":"pcr-basic2","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/pcr-basic2\/","title":{"rendered":"PCR\u6761\u4ef6\u306e\u6700\u9069\u5316\u3063\u3066\u3069\u3046\u3084\u308b\u306e\uff1f PCR\u304c\u521d\u3081\u3066\u306e\u4eba\u306b\u6559\u3048\u3066\u3042\u3052\u305f\u3044\u3061\u3087\u3063\u3068\u3057\u305f\u30b3\u30c4\u305d\u306e2"},"content":{"rendered":"<p>\u524d\u56de\u306fPCR\u306b\u304a\u3051\u308b\u53ce\u91cf\u3001\u7279\u7570\u6027\u3001\u30d5\u30a3\u30c7\u30ea\u30c6\u30a3\u3068\u9175\u7d20\u306e\u95a2\u4fc2\u306b\u3064\u3044\u3066\u3054\u7d39\u4ecb\u3057\u307e\u3057\u305f\u3002\u4eca\u56de\u306f\u3001\u305d\u308c\u3089\u306e\u7279\u9577\u3092\u3075\u307e\u3048\u3066PCR\u306e\u6700\u9069\u5316\u306b\u3064\u3044\u3066\u8a18\u3057\u307e\u3059\u3002<\/p>\n<div class=\"box box-blue\"><strong>PCR\u304c\u3046\u307e\u304f\u5897\u5e45\u3057\u306a\u3044\u3068\u304d\u3001\u5fc5\u8981\u306a\u3044\u7523\u7269\u304c\u5897\u3048\u3066\u3057\u307e\u3063\u305f\u3068\u304d\u3001\u307e\u305f\u306f\u5168\u304f\u4f55\u3082\u5897\u3048\u306a\u3044\u3068\u304d\u3001\u3053\u306e\u3088\u3046\u306a\u3068\u304d\u306b\u3001\u3069\u306e\u3088\u3046\u306a\u4f5c\u696d\u3092\u884c\u3048\u3070\u3088\u3044\u306e\u3067\u3057\u3087\u3046\u304b\uff1f<\/strong><\/div>\n<p>\u307e\u305a\u306f\u3001\u305d\u308c\u3089\u306b\u95a2\u308f\u308b\u30d1\u30e9\u30e1\u30fc\u30bf\u3092\u7406\u89e3\u3059\u308b\u3068\u3001\u3088\u308a\u30b9\u30e0\u30fc\u30ba\u306b\u6700\u9069\u5316\u304c\u3067\u304d\u308b\u3068\u601d\u3044\u307e\u3059\u3002<\/p>\n<h2>\u5869\u6fc3\u5ea6\u306e\u91cd\u8981\u6027<\/h2>\n<p>\u307e\u305a\u3001\u9175\u7d20\u306f2\u4fa1\u306e\u967d\u30a4\u30aa\u30f3\u3092\u8981\u6c42\u3057\u307e\u3059\u3002PCR\u6eb6\u6db2\u306b\u30de\u30b0\u30cd\u30b7\u30a6\u30e0\u6eb6\u6db2\u304c\u5165\u3063\u3066\u3044\u308b\u3053\u3068\u304b\u3089\u3082\u5206\u304b\u308b\u3068\u601d\u3044\u307e\u3059\u3002\u3067\u306f\u3001\u30de\u30b0\u30cd\u30b7\u30a6\u30e0\u6fc3\u5ea6\u304c\u9ad8\u304f\u306a\u308b\u3068\u3069\u3046\u306a\u308b\u304b\u3068\u3044\u3046\u3068\u3001\u9175\u7d20\u306e\u6d3b\u6027\u306f\u5f53\u7136\u9ad8\u304f\u306a\u308a\u3001\u53ce\u91cf\u3082\u5897\u3048\u307e\u3059\u3002\u3057\u304b\u3057\u904e\u5270\u306a\u6d3b\u6027\u306f\u7279\u7570\u6027\u3092\u5931\u3046\u3060\u3051\u3067\u306a\u304f\u3001\u4e0d\u8981\u306aPCR\u7523\u7269\u3082\u5897\u5e45\u3059\u308b\u3053\u3068\u306b\u306a\u308a\u307e\u3059\u3002\u307e\u305f\u3001\u9ad8\u3044\u9175\u7d20\u6d3b\u6027\u306f\u3001\u30d5\u30a3\u30c7\u30ea\u30c6\u30a3\u306e\u4f4e\u4e0b\u306b\u3082\u3064\u306a\u304c\u308a\u307e\u3059\u3002\u3064\u307e\u308a<strong>\u5fc5\u8981\u306a\u3044\u7523\u7269\u304c\u5897\u3048\u3066\u3057\u307e\u3063\u305f\u3068\u304d\u306f\u3001\u30de\u30b0\u30cd\u30b7\u30a6\u30e0\u6fc3\u5ea6\u3092\u4f4e\u304f\u8a2d\u5b9a\u3059\u308b\u3068\u3001\u76ee\u7684\u306e\u7523\u7269\u306e\u307f\u304c\u6b8b\u308b\u3088\u3046\u306b\u6700\u9069\u5316\u3092\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002<\/strong>\u9006\u306b\u3001<strong>\u53ce\u91cf\u304c\u5c11\u306a\u3044\u5834\u5408\u306f\u3001\u5897\u3084\u3059\u65b9\u5411\u3067\u306e\u8abf\u6574\u304c\u5fc5\u8981\u306b\u306a\u308a\u307e\u3059\u3002<\/strong>\u4e00\u822c\u7684\u306a\u81f3\u9069\u6700\u7d42\u6fc3\u5ea6\u306f1.5\uff5e2.5 mM\u3068\u306f\u8a00\u308f\u308c\u3066\u3044\u307e\u3059\u304c\u3001PCR\u7523\u7269\u306e\u9396\u9577\u304c\u9577\u304f\u306a\u308c\u3070\u306a\u308b\u307b\u3069\u3001\u305d\u306e\u81f3\u9069\u5869\u6fc3\u5ea6\u7bc4\u56f2\u306f\u72ed\u304f\u306a\u308a\u307e\u3059\u3002<\/p>\n<h2>\u6e29\u5ea6\u30d7\u30ed\u30d5\u30a1\u30a4\u30eb\u306e\u5de5\u592b<\/h2>\n<h3>2\u70b9\uff1f\u305d\u308c\u3068\u30823\u70b9PCR\uff1f<\/h3>\n<p>PCR\u306e\u57fa\u790e\u3067\u306f3\u3064\u306e\u6e29\u5ea6\uff08Denature-Annealing-Extension\uff09\u3092\u8a2d\u5b9a\u3059\u308b\u3053\u3068\u304b\u3089\u306f\u3058\u307e\u308a\u307e\u3059\u3002\u3057\u304b\u3057\u7279\u6b8a\u306a\u6761\u4ef6\u3067\u306a\u3051\u308c\u3070\u3001\u3053\u306e3\u3064\u306e\u6e29\u5ea6\u3092\u8a2d\u5b9a\u3059\u308b\u306e\u306f\u904e\u5270\u3067\u3042\u308b\u3068\u8a00\u3048\u307e\u3059\u3002\u3064\u307e\u308a\u3001<strong>\u30d7\u30e9\u30a4\u30de\u30fc\u3092Annealing\u3055\u305b\u308b\u30b9\u30c6\u30c3\u30d7\u3067\u3082\u9175\u7d20\u306e\u6d3b\u6027\uff0850\u2103\u30671\uff5e2 kb\/min\uff09\u306f\u5341\u5206\u306b\u3042\u308b\u3068\u3044\u3046\u3053\u3068<\/strong>\u3067\u3059\u3002\u3053\u306e\u6d3b\u6027\u3092\u307f\u308c\u3070\u3001Annealing\u306730\u79d2\u7a0b\u5ea6\u6e29\u5ea6\u4fdd\u6301\u3092\u884c\u3046\u3060\u3051\u3067\u3001\u6570100bp\u306e\u4f38\u9577\u53cd\u5fdc\u306f\u884c\u308f\u308c\u3066\u3044\u308b\u3068\u3044\u3046\u3053\u3068\u3067\u3059\u3002\u305d\u306e\u5f8c\u306bExtension\u30b9\u30c6\u30c3\u30d7\u3068\u3057\u3066\u3001\u3055\u3089\u306b72\u2103\u3067\u657010\u79d2\u884c\u3046\u3053\u3068\u306f\u3001\u4e00\u822c\u7684\u306a500bp\u7a0b\u5ea6\u306ePCR\u306b\u304a\u3044\u3066\u306f\u904e\u5270\u3067\u3042\u308b\u3068\u8a00\u308f\u3056\u308b\u3092\u5f97\u307e\u305b\u3093\u3002<\/p>\n<p>\u307e\u305f\u3001\u5404\u30b9\u30c6\u30c3\u30d7\u9593\u3067\u306e\u79fb\u52d5\u3001\u305f\u3068\u3048\u3070Annealing\u304b\u3089Extension\u3078\u3001\u79fb\u884c\u3059\u308b\u6642\u9593\u3082\u4f38\u9577\u53cd\u5fdc\u306f\u884c\u308f\u308c\u307e\u3059\u3002\u305d\u306e\u305f\u3081<strong>\u6e29\u5ea6\u79fb\u884c\u901f\u5ea6\u306e\u7570\u306a\u308b\u6a5f\u7a2e\u9593\u3067\u306f\u3001\u6e29\u5ea6\u30d7\u30ed\u30d5\u30a1\u30a4\u30eb\u304c\u540c\u3058\u3067\u3082\u7d50\u679c\u304c\u7570\u306a\u308b\u306e\u306f\u3001\u3053\u3046\u3044\u3063\u305f\u3053\u3068\u304c\u539f\u56e0\u3067\u3042\u308b\u3053\u3068\u304c\u591a\u304f\u3042\u308a\u307e\u3059\u3002<\/strong>\u3057\u305f\u304c\u3063\u3066\u3001\u3053\u308c\u304b\u3089\u4e00\u822c\u7684\u306aPCR\u3092\u306f\u3058\u3081\u3088\u3046\u3068\u3044\u3046\u65b9\u306f\u3001<strong>Annealing\u3068Extension\u3092\u4e00\u7dd2\u306b\u3057\u305fDenature-Annealing\/ Extension\u306e2\u70b9PCR\u3092\u63a1\u7528\u3059\u308b\u3053\u3068\u3092\u304a\u52e7\u3081\u3057\u307e\u3059\u3002<\/strong><\/p>\n<h3>\u30a2\u30cb\u30fc\u30ea\u30f3\u30b0\u6e29\u5ea6\u306e\u8a2d\u5b9a<\/h3>\n<p>PCR\u306e\u6e29\u5ea6\u8a2d\u5b9a\u3092\u884c\u3046\u5834\u5408\u30012\u70b9\u3067\u30823\u70b9\u3067\u3082Denature\u306e\u6e29\u5ea6\u306f\u307b\u307c95\u2103\u524d\u5f8c\u3068\u306a\u308a\u307e\u3059\u3002\u307e\u305f3\u70b9PCR\u3067\u306eExtension\u306f72\u2103\u3068\u3057\u307e\u3059\u3002\u3057\u305f\u304c\u3063\u3066\u3001<strong>\u8a2d\u5b9a\u3059\u308b\u5fc5\u8981\u304c\u3042\u308b\u306e\u306f\u3001Annealing\u6e29\u5ea6\u306e\u307f\u3067\u3059\u3002<\/strong>\u4e00\u822c\u306b\u30d7\u30e9\u30a4\u30de\u30fc\u306eTm\u5024\u304b\u30892\uff5e3\u2103\u7a0b\u5ea6\u4f4e\u3044\u6e29\u5ea6\u3068\u3055\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u307e\u305a\u306fTm\u5024\u306b\u95a2\u3057\u3066\u7c21\u5358\u306b\u89e3\u8aac\u3057\u307e\u3059\u3002Tm\u5024\u306f\u878d\u89e3\u6e29\u5ea6\u3068\u8a33\u3055\u308c\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u304c\u30bf\u30fc\u30b2\u30c3\u30c8\u914d\u5217\u306850\uff05\u306e\u5272\u5408\u30672\u672c\u9396\u3092\u5f62\u6210\u3059\u308b\u6e29\u5ea6\u3067\u3059\u3002\u305d\u306e\u305f\u3081\u6e29\u5ea6\u304c\u4f4e\u3044\u65b9\u304c2\u672c\u9396\u3092\u5f62\u6210\u3057\u3066\u3044\u308b\u6bd4\u7387\u304c\u9ad8\u304f\u306a\u308b\u3068\u8a00\u3048\u307e\u3059\u3002\u307e\u305fTm\u5024\u3088\u308a\u3082\u9ad8\u3044\u6e29\u5ea6\u3067\u3042\u3063\u305f\u3068\u3057\u3066\u3082\u30012\u672c\u9396\u306e\u5272\u5408\u304c\u3059\u304f\u306a\u304f\u306a\u308b\u3060\u3051\u3067\u3001Annealing\u306f\u304a\u3053\u3063\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>\u3055\u3066\u3001Annealing\u6e29\u5ea6\u306e\u8a2d\u5b9a\u3067\u3059\u304c\u3001PCR\u30d7\u30e9\u30a4\u30de\u30fc\u306f2\u672c\u4f7f\u7528\u3057\u307e\u3059\u306e\u3067\u30012\u3064\u306eTm\u5024\u304cPCR\u6761\u4ef6\u306b\u306f\u5b58\u5728\u3059\u308b\u3053\u3068\u306b\u306a\u308a\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u3001<strong>\u305d\u308c\u3089\u6e29\u5ea6\u306e\u4e2d\u70b9\u306b\u8a2d\u5b9a\u3059\u308b\u3068\u3053\u308d\u304b\u3089\u30b9\u30bf\u30fc\u30c8\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u305d\u306e\u7406\u7531\u3068\u3057\u3066\u306f\u3001 2\u7a2e\u985e\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u306eAnnealing\u983b\u5ea6\u3092\u8fd1\u4f3c\u3055\u305b\u308b\u3053\u3068\u3067\u3059\u3002<\/strong>\u305d\u3057\u3066<strong>\u975e\u7279\u7570\u7684\u306aPCR\u7523\u7269\u304c\u51fa\u308b\u3088\u3046\u3067\u3057\u305f\u3089\u3001Tm\u5024\u4ee5\u4e0a\u3067\u3082\u69cb\u3044\u307e\u305b\u3093\u306e\u3067\u3001\u8a2d\u5b9a\u6e29\u5ea6\u3092\u4e0a\u3052\u3066\u304f\u3060\u3055\u3044\u3002<\/strong>\u3053\u308c\u306b\u3088\u308a\u30d7\u30e9\u30a4\u30de\u30fc\u306eAnnealing\u52b9\u7387\u306f\u4e0b\u304c\u308a\u307e\u3059\u304c\u3001\u6e29\u5ea6\u4e0a\u6607\u306b\u3057\u305f\u304c\u3063\u3066\u9175\u7d20\u3082\u6d3b\u6027\u304c\u4e0a\u304c\u308a\u307e\u3059\u3002 \u7279\u7570\u7684PCR\u5897\u5e45\u304c\u884c\u308f\u308c\u308bAnnealing\u6e29\u5ea6\u304c\u8a2d\u5b9a\u3067\u304d\u305f\u5f8c\u306b\u3001\u3055\u3089\u306a\u308b\u53ce\u91cf\u304c\u5fc5\u8981\u3067\u3042\u308c\u3070\u3001\u5869\u6fc3\u5ea6\u3092\u4e0a\u3052\u3066\u8abf\u6574\u3059\u308c\u3070\u3088\u3044\u304b\u3068\u601d\u308f\u308c\u307e\u3059\u3002<\/p>\n<h2>\u6642\u9593\u306e\u8a2d\u5b9a<\/h2>\n<p>\u5404\u30b9\u30c6\u30c3\u30d7\u3067\u306e\u4fdd\u6301\u6642\u9593\u3067\u3059\u304c\u3001<strong>\u307e\u305aDenature\u30b9\u30c6\u30c3\u30d7\u3067\u3059\u3002\u3053\u3053\u3067\u306f30\u79d2\u7a0b\u5ea6\u3067\u5341\u5206\u3067\u3059\u3002<\/strong>\u9577\u9396\u306e\u30b2\u30ce\u30e0DNA\u3067\u3055\u3048\u300120\u79d2\u7a0b\u5ea6\u30671\u672c\u9396\u306b\u89e3\u96e2\u3059\u308b\u3068\u8a00\u308f\u308c\u3066\u3044\u307e\u3059\u3002\u307e\u305f\u3001PCR\u3067\u306e\u672c\u5f53\u306e\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u306b\u306a\u308b\u306e\u306f\u3001\u524d\u30b5\u30a4\u30af\u30eb\u3067\u5897\u5e45\u3057\u305fPCR\u7523\u7269\u305d\u306e\u3082\u306e\u3067\u3001\u305d\u306e\u9396\u9577\u306f\u30b2\u30ce\u30e0DNA\u306b\u6bd4\u3079\u308c\u3070\u306f\u308b\u304b\u306b\u77ed\u304f\u3001\u7c21\u5358\u306b\u89e3\u96e2\u3057\u307e\u3059\u3002<strong>Annealing\u306b\u304a\u3044\u3066\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u5206\u5b50\u6570\u306f\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u306b\u306a\u308bDNA\u3088\u308a\u306f\u308b\u304b\u306b\u591a\u304f\u5e38\u306b\u4f1a\u5408-\u89e3\u96e2\u3092\u7e70\u308a\u8fd4\u3057\u3066\u3044\u307e\u3059\u306e\u3067\u300130\u79d2\u3082\u3042\u308c\u30702\u672c\u9396\u3092\u5f62\u6210\u3057\u3001\u5c11\u3057\u306e\u4f38\u9577\u307e\u3067\u304a\u304d\u307e\u3059\u30022\u70b9PCR\u306b\u304a\u3044\u3066\u306f\u3001\u3053\u3053\u3067\u4f38\u9577\u53cd\u5fdc\u3082\u884c\u3044\u307e\u3059\u306e\u3067\u3001\u304a\u304a\u3080\u306d\u3067\u3059\u304c40\u79d2\/kb\u3068\u3057\u3066\u8a2d\u5b9a\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/strong>\u8a08\u7b97\u4e0a30\u79d2\u4ee5\u4e0b\u306b\u306a\u308b\u5834\u5408\u306f30\u79d2\u3068\u3057\u3066\u8a2d\u5b9a\u3092\u3059\u308b\u3053\u3068\u3092\u304a\u52e7\u3081\u3057\u307e\u3059\u3002<\/p>\n<h2>\u305d\u308c\u3067\u3082\u3046\u307e\u304f\u5897\u5e45\u304c\u3067\u304d\u306a\u3044\u2026\u2026<\/h2>\n<p>\u3053\u308c\u306f\u304a\u305d\u3089\u304f\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u81ea\u8eab\u306e\u554f\u984c\u3067\u3059\u3002\u3053\u308c\u306f\u6b21\u56de\u306b\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n<h2>\u3053\u308c\u3060\u3051\u306f\u77e5\u3063\u3066\u304a\u304d\u305f\u3044PCR\u306e\u57fa\u790e\u77e5\u8b58\u3001\u30b3\u30b3\u306b\u3042\u308a\u307e\u3059\uff01<\/h2>\n<p>Invitrogen \u5206\u5b50\u751f\u7269\u5b66\u6559\u5ba4\u3067\u306f\u3001\u5206\u5b50\u751f\u7269\u5b66\u306e\u6559\u80b2\u306e\u30e1\u30a4\u30f3\u3068\u3057\u3066\u3001\u521d\u5fc3\u8005\u3060\u3051\u3067\u306a\u304f\u7d4c\u9a13\u306e\u3042\u308b\u5206\u5b50\u751f\u7269\u5b66\u8005\u306e\u305f\u3081\u306b\u3001\u8c4a\u5bcc\u3067\u4fe1\u983c\u3067\u304d\u308b\u6280\u8853\u7684\u306a\u5185\u5bb9\u3092\u3054\u63d0\u4f9b\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>PCR\u306e\u57fa\u790e\u306e\u5fa9\u7fd2\u3084\u3001\u3088\u308a\u6df1\u3044\u77e5\u8b58\u306e\u7372\u5f97\u3092\u76ee\u7684\u3068\u3057\u305f\u7121\u6599PDF\u3082\u63d0\u4f9b\u3057\u3066\u304a\u308a\u307e\u3059\u306e\u3067\u304a\u6c17\u8efd\u306b\u3054\u89a7\u304f\u3060\u3055\u3044\uff01<\/p>\n<p>\u25bc\u4e3b\u306a\u5185\u5bb9<\/p>\n<ul>\n<li>PCR\u306e\u57fa\u790e\u77e5\u8b58<\/li>\n<li>PCR\u306e\u30bb\u30c3\u30c8\u30a2\u30c3\u30d7\u3067\u8003\u616e\u3059\u3079\u304d6\u3064\u306e\u8981\u7d20<\/li>\n<li>PCR\u53cd\u5fdc\u6761\u4ef6\u306b\u304a\u3051\u308b6 \u3064\u306e\u6ce8\u610f\u70b9<\/li>\n<li>DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306e4\u3064\u306e\u4e3b\u306a\u7279\u6027<\/li>\n<li>\u4e00\u822c\u7684\u306a10\u306ePCR\u6cd5<\/li>\n<li>PCR\u3092\u7528\u3044\u305f7\u3064\u306e\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3<\/li>\n<li>PCR\u306b\u95a2\u3059\u308b\u30c8\u30e9\u30d6\u30eb\u30b7\u30e5\u30fc\u30c6\u30a3\u30f3\u30b0\u30ac\u30a4\u30c9<\/li>\n<li>\u7cbe\u88fd\u30b0\u30ec\u30fc\u30c9\u9078\u629e\u306e\u30ac\u30a4\u30c0\u30f3\u30b9<\/li>\n<\/ul>\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/mol-bio-school.html?CID=bid_mol_pch_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pcr4_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u7528\u306b\u306f\u4f7f\u7528\u3044\u305f\u3060\u3051\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>PCR\u304c\u3046\u307e\u304f\u5897\u5e45\u3057\u306a\u3044\u3068\u304d\u3001\u5fc5\u8981\u306a\u3044\u7523\u7269\u304c\u5897\u3048\u3066\u3057\u307e\u3063\u305f\u3068\u304d\u3001\u307e\u305f\u306f\u5168\u304f\u4f55\u3082\u5897\u3048\u306a\u3044\u3068\u304d\u3001\u3053\u306e\u3088\u3046\u306a\u3068\u304d\u306b\u3001\u3069\u306e\u3088\u3046\u306a\u4f5c\u696d\u3092\u884c\u3048\u3070\u3088\u3044\u306e\u3067\u3057\u3087\u3046\u304b\uff1f\u4eca\u56de\u306f\u3001PCR\u306b\u304a\u3051\u308b\u53ce\u91cf\u3001\u7279\u7570\u6027\u3001\u30d5\u30a3\u30c7\u30ea\u30c6\u30a3\u3068\u9175\u7d20\u306e\u95a2\u4fc2\u3092\u3075\u307e\u3048\u3066PCR\u306e\u6700\u9069\u5316\u306b\u3064\u3044\u3066\u3054\u7d39\u4ecb\u3057\u307e\u3059\uff01<\/p>\n","protected":false},"author":988,"featured_media":2330,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[156],"tags":[9],"division":[],"class_list":{"0":"post-211","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-mol-bio","8":"tag-pcr","9":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.8 (Yoast SEO v27.8) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>PCR\u6761\u4ef6\u306e\u6700\u9069\u5316\u3063\u3066\u3069\u3046\u3084\u308b\u306e\uff1f PCR\u304c\u521d\u3081\u3066\u306e\u4eba\u306b\u6559\u3048\u3066\u3042\u3052\u305f\u3044\u3061\u3087\u3063\u3068\u3057\u305f\u30b3\u30c4\u305d\u306e2 - Learning at the Bench<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/pcr-basic2\/\" \/>\n<meta property=\"og:locale\" content=\"ja_JP\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"PCR\u6761\u4ef6\u306e\u6700\u9069\u5316\u3063\u3066\u3069\u3046\u3084\u308b\u306e\uff1f PCR\u304c\u521d\u3081\u3066\u306e\u4eba\u306b\u6559\u3048\u3066\u3042\u3052\u305f\u3044\u3061\u3087\u3063\u3068\u3057\u305f\u30b3\u30c4\u305d\u306e2\" \/>\n<meta property=\"og:description\" content=\"PCR\u304c\u3046\u307e\u304f\u5897\u5e45\u3057\u306a\u3044\u3068\u304d\u3001\u5fc5\u8981\u306a\u3044\u7523\u7269\u304c\u5897\u3048\u3066\u3057\u307e\u3063\u305f\u3068\u304d\u3001\u307e\u305f\u306f\u5168\u304f\u4f55\u3082\u5897\u3048\u306a\u3044\u3068\u304d\u3001\u3053\u306e\u3088\u3046\u306a\u3068\u304d\u306b\u3001\u3069\u306e\u3088\u3046\u306a\u4f5c\u696d\u3092\u884c\u3048\u3070\u3088\u3044\u306e\u3067\u3057\u3087\u3046\u304b\uff1f\u4eca\u56de\u306f\u3001PCR\u306b\u304a\u3051\u308b\u53ce\u91cf\u3001\u7279\u7570\u6027\u3001\u30d5\u30a3\u30c7\u30ea\u30c6\u30a3\u3068\u9175\u7d20\u306e\u95a2\u4fc2\u3092\u3075\u307e\u3048\u3066PCR\u306e\u6700\u9069\u5316\u306b\u3064\u3044\u3066\u3054\u7d39\u4ecb\u3057\u307e\u3059\uff01\" \/>\n<meta property=\"og:url\" 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PCR\u304c\u521d\u3081\u3066\u306e\u4eba\u306b\u6559\u3048\u3066\u3042\u3052\u305f\u3044\u3061\u3087\u3063\u3068\u3057\u305f\u30b3\u30c4\u305d\u306e2\",\"datePublished\":\"2015-12-09T15:00:00+00:00\",\"dateModified\":\"2024-08-27T02:35:20+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/pcr-basic2\\\/\"},\"wordCount\":71,\"commentCount\":0,\"image\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/pcr-basic2\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/learning-at-the-bench\\\/wp-content\\\/uploads\\\/sites\\\/13\\\/2019\\\/07\\\/pcr-basic-title-1.jpg\",\"keywords\":[\"PCR\"],\"articleSection\":[\"\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u95a2\u9023\"],\"inLanguage\":\"ja\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/pcr-basic2\\\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/pcr-basic2\\\/\",\"url\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/learning-at-the-bench\\\/pcr-basic2\\\/\",\"name\":\"PCR\u6761\u4ef6\u306e\u6700\u9069\u5316\u3063\u3066\u3069\u3046\u3084\u308b\u306e\uff1f 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