{"id":213,"date":"2020-03-04T00:00:00","date_gmt":"2020-03-03T15:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic18\/"},"modified":"2025-07-24T19:32:27","modified_gmt":"2025-07-24T10:32:27","slug":"protein-basic18","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic18\/","title":{"rendered":"\u7d44\u7e54\u67d3\u8272\u306b\u304a\u3051\u308b\u56fa\u5b9a\u5316\u307e\u3068\u3081\uff5c\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\uff01\u30bf\u30f3\u30d1\u30af\u8cea\u5b9f\u9a13\u3042\u308c\u3053\u308c \u7b2c18\u56de"},"content":{"rendered":"<p><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic17\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa14_Social_LAB\" target=\"_blank\">\u524d\u56de<\/a>\u306f\u3001\u514d\u75ab\u7d44\u7e54\u5316\u5b66\uff08Immunohistochemistry; IHC\uff09\u306e\u6982\u8981\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3057\u305f\u3002\u4eca\u56de\u306f\u514d\u75ab\u7d44\u7e54\u5316\u5b66\u306b\u304a\u3044\u3066\u91cd\u8981\u306a\u30b9\u30c6\u30c3\u30d7\u3067\u3042\u308b\u56fa\u5b9a\u5316\u306b\u3064\u3044\u3066\u3082\u3046\u5c11\u3057\u8a73\u3057\u3044\u5185\u5bb9\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\uff01<\/p>\n<h2>\u56fa\u5b9a\u5316\u306e\u76ee\u7684<\/h2>\n<p>\u514d\u75ab\u7d44\u7e54\u5316\u5b66\u306b\u304a\u3051\u308b\u56fa\u5b9a\u5316\u306b\u306f\u3001\u6b21\u306e4\u3064\u306e\u76ee\u7684\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<ul>\n<li>\u7d30\u80de\u306e\u5f62\u614b\u3084\u7d44\u7e54\u306e\u69cb\u9020\u3092\u5b89\u5b9a\u5316<\/li>\n<li>\u30bf\u30f3\u30d1\u30af\u8cea\u5206\u89e3\u9175\u7d20\u306e\u4e0d\u6d3b\u5316<\/li>\n<li>\u52a0\u5de5\u3084\u67d3\u8272\u51e6\u7406\u306b\u5bfe\u3059\u308b\u30b5\u30f3\u30d7\u30eb\u306e\u5f37\u5316<\/li>\n<li>\u5fae\u751f\u7269\u306e\u30b3\u30f3\u30bf\u30df\u30cd\u30fc\u30b7\u30e7\u30f3\u3084\u8150\u8755\u3092\u6291\u5236<\/li>\n<\/ul>\n<h2>\u56fa\u5b9a\u5316\u306e\u65b9\u6cd5<\/h2>\n<p>\u514d\u75ab\u7d44\u7e54\u5316\u5b66\u306b\u304a\u3051\u308b\u56fa\u5b9a\u5316\u306f\u3001\u5206\u5b50\u67b6\u6a4b\u3084\u30bf\u30f3\u30d1\u30af\u8cea\u4e0d\u6eb6\u5316\u306b\u3088\u308b\u5316\u5b66\u7684\u306a\u65b9\u6cd5\u3067\u884c\u3044\u307e\u3059\u3002\u307e\u305f\u3001\u30b5\u30f3\u30d7\u30eb\u3084\u6a19\u7684\u6297\u539f\u306b\u3088\u3063\u3066\u306f\u7269\u7406\u7684\u306a\u65b9\u6cd5\u3067\u884c\u3046\u3053\u3068\u3082\u3042\u308a\u307e\u3059\u3002\u4f8b\u3048\u3070\u8840\u6db2\u5857\u62b9\u6a19\u672c\u306e\u5834\u5408\u3001\u4e7e\u71e5\u6cd5\u306b\u3088\u3063\u3066\u56fa\u5b9a\u5316\u3057\u307e\u3059\u3002\u5316\u5b66\u7684\u306a\u56fa\u5b9a\u5316\u3084\u6709\u6a5f\u6eb6\u5a92\uff08\u8131\u30d1\u30e9\u30d5\u30a3\u30f3\u51e6\u7406\u3067\u4f7f\u7528\uff09\u306b\u3088\u308a\u30b5\u30f3\u30d7\u30eb\u3084\u6297\u539f\u304c\u30c0\u30e1\u30fc\u30b8\u3092\u53d7\u3051\u308b\u5834\u5408\u306b\u306f\u51cd\u7d50\u6cd5\u306b\u3088\u3063\u3066\u56fa\u5b9a\u5316\u3057\u307e\u3059\u3002\u56fa\u5b9a\u5316\u306b\u306f\u6b21\u306e4\u3064\u306e\u65b9\u6cd5\u304c\u5229\u7528\u3055\u308c\u307e\u3059\u3002<\/p>\n<ul>\n<li>\u6d78\u6f2c\u6cd5\uff1a \u30b5\u30f3\u30d7\u30eb\u3092\u56fa\u5b9a\u6db2\u306b\u6d78\u3051\u3066\u56fa\u5b9a\u5316\u3059\u308b\u65b9\u6cd5<\/li>\n<li>\u704c\u6d41\u6cd5\uff1a \u63a1\u8840\u3057\u3066\u751f\u7406\u98df\u5869\u6c34\u3092\u704c\u6d41\u3057\u305f\u5f8c\u3001\u56fa\u5b9a\u6db2\u3092\u704c\u6d41\u3059\u308b\u3053\u3068\u3067\u6df1\u90e8\u7d44\u7e54\u3092\u8fc5\u901f\u306b\u56fa\u5b9a\u5316\u3059\u308b\u65b9\u6cd5<\/li>\n<li>\u51cd\u7d50\u6cd5\uff1a \u4f4e\u6e29\u51cd\u7d50\u7528\u5305\u57cb\u5264\uff08OCT\u30b3\u30f3\u30d1\u30a6\u30f3\u30c9\u306a\u3069\uff09\u306b\u6d78\u3057\u3066\u304b\u3089\u51cd\u7d50\u3057\u6db2\u4f53\u7a92\u7d20\u4e2d\u3067\u4fdd\u5b58\u3059\u308b\u65b9\u6cd5<\/li>\n<li>\u4e7e\u71e5\u6cd5\uff1a \u30b5\u30f3\u30d7\u30eb\u3092\u98a8\u4e7e\u3057\u3066\u304b\u3089\u708e\u306a\u3069\u306b\u3088\u308a\u30b9\u30e9\u30a4\u30c9\u4e0a\u306b\u52a0\u71b1\u56fa\u5b9a\u3059\u308b\u65b9\u6cd5<\/li>\n<\/ul>\n<p><strong>\u56fa\u5b9a\u5316\u306b\u3088\u308a\u6a19\u7684\u6297\u539f\u304c\u30de\u30b9\u30af\u3055\u308c\u3066\u3057\u307e\u3063\u3066\u306f\u6297\u4f53\u304c\u30a2\u30af\u30bb\u30b9\u3067\u304d\u305a\u691c\u51fa\u3067\u304d\u306a\u304f\u306a\u3063\u3066\u3057\u307e\u3044\u307e\u3059\u3002\u3057\u305f\u304c\u3063\u3066\u3001\u6297\u539f\u691c\u51fa\uff08\u67d3\u8272\uff09\u304c\u884c\u3048\u308b\u3088\u3046\u306b\u9069\u5207\u306a\u56fa\u5b9a\u5316\u3092\u884c\u3046\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002<\/strong><\/p>\n<h2>\u56fa\u5b9a\u5316\u306e\u6761\u4ef6<\/h2>\n<p>\u7279\u5b9a\u306e\u56fa\u5b9a\u5316\u6761\u4ef6\u304c\u3001\u3042\u308b\u6297\u539f\u30a8\u30d4\u30c8\u30fc\u30d7\u306e\u6297\u539f\u6027\u4fdd\u6301\u306b\u9069\u3057\u3066\u3044\u3066\u3082\u3001\uff08\u305f\u3068\u3048\u540c\u3058\u6297\u539f\u3067\u3042\u3063\u3066\u3082\uff09\u5225\u306e\u30a8\u30d4\u30c8\u30fc\u30d7\u306b\u306f\u9069\u3055\u306a\u3044\u5834\u5408\u304c\u3042\u308a\u3001<strong>\u6297\u539f\u3084\u305d\u306e\u30a8\u30d4\u30c8\u30fc\u30d7\u306b\u3088\u3063\u3066\u56fa\u5b9a\u5316\u306e\u81f3\u9069\u6761\u4ef6\u306f\u3055\u307e\u3056\u307e\u3067\u3059\u3002<\/strong>\u56fa\u5b9a\u6db2\u3092\u9078\u629e\u3059\u308b\u969b\u306b\u306f\u6b21\u306e\u6761\u4ef6\u3092\u691c\u8a0e\u9805\u76ee\u306b\u304a\u304f\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<ul>\n<li>\u56fa\u5b9a\u6db2\u306e\u7a2e\u985e\uff08\u30db\u30eb\u30e0\u30a2\u30eb\u30c7\u30d2\u30c9\u3001\u30b0\u30eb\u30bf\u30eb\u30a2\u30eb\u30c7\u30d2\u30c9\u306a\u3069\uff09<\/li>\n<li>\u6d78\u6f2c\u3084\u56fa\u5b9a\u5316\u306e\u6642\u9593<\/li>\n<li>\u56fa\u5b9a\u6db2\u306e\u6fc3\u5ea6<\/li>\n<li>pH<\/li>\n<li>\u6e29\u5ea6<\/li>\n<li>\u56fa\u5b9a\u5316\u5f8c\u306e\u51e6\u7406\u65b9\u6cd5<\/li>\n<\/ul>\n<h2>\u5404\u7a2e\u56fa\u5b9a\u5316\u8a66\u85ac\u306b\u3064\u3044\u3066<\/h2>\n<h3>\u30db\u30eb\u30e0\u30a2\u30eb\u30c7\u30d2\u30c9<\/h3>\n<p>\u6700\u3082\u5e83\u304f\u5229\u7528\u3055\u308c\u308b\u67b6\u6a4b\u5264\u304c\u30db\u30eb\u30e0\u30a2\u30eb\u30c7\u30d2\u30c9\u3067\u3059\u3002\u30db\u30eb\u30e0\u30a2\u30eb\u30c7\u30d2\u30c9\u306f\u30bf\u30f3\u30d1\u30af\u8cea\u3084\u6838\u9178\u306e\u30a2\u30df\u30ce\u57fa\uff081\u7d1a\u30a2\u30df\u30f3\u3001-NH2\uff09\u3068\u53cd\u5fdc\u3057\u3066\u90e8\u5206\u7684\u306b\u53ef\u9006\u7684\u306a\u30e1\u30c1\u30ec\u30f3\u7d50\u5408\u3092\u5f62\u6210\u3057\u307e\u3059\u3002\u901a\u5e38\u3001\u30db\u30eb\u30e0\u30a2\u30eb\u30c7\u30d2\u30c9\u306e\u5e02\u8ca9\u54c1\u306f\u3001\u30d1\u30e9\u30db\u30eb\u30e0\u30a2\u30eb\u30c7\u30d2\u30c9\uff08\u30db\u30eb\u30e0\u30a2\u30eb\u30c7\u30d2\u30c9\u306e\u30dd\u30ea\u30de\u30fc\uff09\u304b\u3089\u8abf\u88fd\u3057\u307e\u3059\u304c\u3001\u9178\u5316\u3057\u3066\u30ae\u9178\u3092\u751f\u3058\u308b\u3053\u3068\u3084\u518d\u91cd\u5408\u3059\u308b\u306e\u3092\u9632\u3050\u305f\u3081\u306b\u5b89\u5b9a\u5316\u5264\u3068\u3057\u3066\u30e1\u30bf\u30ce\u30fc\u30eb\uff0810%\u7a0b\u5ea6\uff09\u304c\u6dfb\u52a0\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u30d7\u30ed\u30c8\u30b3\u30eb\u306b\u3088\u3063\u3066\u306f\u3001<strong>\u30db\u30eb\u30e0\u30a2\u30eb\u30c7\u30d2\u30c9\u3092\u7528\u3044\u305f\u56fa\u5b9a\u6db2\u3078\u306e\u30e1\u30bf\u30ce\u30fc\u30eb\u306e\u6301\u3061\u8fbc\u307f\u3092\u907f\u3051\u308b\u305f\u3081\u306b\u30d1\u30e9\u30db\u30eb\u30e0\u30a2\u30eb\u30c7\u30d2\u30c9\u304b\u3089\u7528\u4e8b\u8abf\u88fd\u3057\u305f\u30d5\u30ec\u30c3\u30b7\u30e5\u306a\u30db\u30eb\u30e0\u30a2\u30eb\u30c7\u30d2\u30c9\u306e\u4f7f\u7528\u304c\u63a8\u5968\u3055\u308c\u307e\u3059\u3002<\/strong>\u5f0a\u793e\u3067\u306f\u30e1\u30bf\u30ce\u30fc\u30eb\u30d5\u30ea\u30fc\u306e<a href=\"http:\/\/www.thermofisher.com\/order\/catalog\/product\/28906?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa14_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">16% Formaldehyde<\/a>\u3092\u3054\u7528\u610f\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<h3>\u30b0\u30eb\u30bf\u30eb\u30a2\u30eb\u30c7\u30d2\u30c9<\/h3>\n<p>\u30a2\u30eb\u30c7\u30d2\u30c9\u57fa\u30922\u3064\u3082\u3064\u30b0\u30eb\u30bf\u30eb\u30a2\u30eb\u30c7\u30d2\u30c9\u306f\u3001\u30a2\u30df\u30ce\u57fa\uff08-NH2\uff09\u3084\u30b9\u30eb\u30d5\u30d2\u30c9\u30ea\u30eb\u57fa(-SH)\uff08\u304a\u3088\u3073\u82b3\u9999\u74b0\uff09\u3068\u53cd\u5fdc\u3059\u308b\u305f\u3081\u3001\u30db\u30eb\u30e0\u30a2\u30eb\u30c7\u30d2\u30c9\u3088\u308a\u5f37\u529b\u306a\u67b6\u6a4b\u5264\u3068\u3044\u3048\u307e\u3059\u304c\u3001<strong>\u7d44\u7e54\u3078\u306e\u6d78\u900f\u304c\u9045\u304f\u6c34\u6eb6\u6027\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u904a\u96e2\u3084\u30b5\u30f3\u30d7\u30eb\u7d44\u7e54\u306e\u69cb\u9020\u5909\u5316\u3092\u3082\u305f\u3089\u3059\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002<\/strong>\u30b0\u30eb\u30bf\u30eb\u30a2\u30eb\u30c7\u30d2\u30c9\u3092\u7528\u3044\u3066\u56fa\u5b9a\u5316\u3092\u884c\u3063\u305f\u5834\u5408\u3001\u56fa\u5b9a\u5316\u5f8c\u306b\u30a2\u30df\u30ce\u57fa\u3092\u6301\u3064\u5206\u5b50\u3067\u51e6\u7406\u3057\u3066\u904a\u96e2\u306e\u30a2\u30eb\u30c7\u30d2\u30c9\u57fa\u3092\u30d6\u30ed\u30c3\u30af\u3057\u307e\u3059\u3002\u3053\u306e\u51e6\u7406\u3092\u3057\u306a\u3044\u3068\u3001\u672a\u53cd\u5fdc\u306e\u30a2\u30eb\u30c7\u30d2\u30c9\u57fa\u304c\u691c\u51fa\u306e\u969b\u306b\u6dfb\u52a0\u3057\u305f\u6297\u4f53\u306a\u3069\u306e\u30a2\u30df\u30ce\u57fa\u3068\u53cd\u5fdc\u3057\u3066\u3057\u307e\u3044\u307e\u3059\u3002\u4e00\u822c\u7684\u306b\u3001\u30a2\u30eb\u30c7\u30d2\u30c9\u57fa\u306e\u30d6\u30ed\u30c3\u30af\u306b\u306f\u30a8\u30bf\u30ce\u30fc\u30eb\u30a2\u30df\u30f3\u3084\u30ea\u30b8\u30f3\u3092\u4f7f\u7528\u3057\u307e\u3059\u3002<\/p>\n<h3>\u30a2\u30eb\u30b3\u30fc\u30eb<\/h3>\n<p>\u30a8\u30bf\u30ce\u30fc\u30eb\u3001\u30e1\u30bf\u30ce\u30fc\u30eb\u3001\u30a2\u30bb\u30c8\u30f3\u306a\u3069\u306f\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u6c88\u6bbf\u3055\u305b\u308b\u4f5c\u7528\u304c\u3042\u308a\u3001\u7d30\u80de\u306e\u4fdd\u5b58\u306b\u6709\u7528\u3067\u3059\u304c\u3001<strong>\u7d44\u7e54\u3092\u53ce\u7e2e\u3055\u305b\u308b\u4f5c\u7528\u304c\u3042\u308b\u305f\u3081\u3001\u96fb\u5b50\u9855\u5fae\u93e1\u306e\u56fa\u5b9a\u306b\u306f\u4e0d\u5411\u304d\u3067\u3059\u3002<\/strong><\/p>\n<h3>\u30b9\u30d9\u30eb\u30a4\u30df\u30ce\u9178\u30b8\u30e1\u30c1\u30eb<\/h3>\n<p>DMS (dimethyl suberimidate)\u306f\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u03b1\u30a2\u30df\u30ce\u57fa\uff08N\u672b\u7aef\uff09\u3084\u03b5\u30a2\u30df\u30ce\u57fa\uff08\u30ea\u30b8\u30f3\u5074\u9396\uff09\u3068\u7d50\u5408\u3059\u308b\u30db\u30e2\u30d0\u30a4\u30d5\u30a1\u30f3\u30af\u30b7\u30e7\u30ca\u30eb\u306a\u67b6\u6a4b\u5264\u3067\u3059\u3002DMS\u306f\u3001\u67b6\u6a4b\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u69cb\u9020\u5909\u5316\u3092\u8d77\u3053\u3057\u306b\u304f\u3044\u305f\u3081<strong>\u6297\u539f\u306e\u514d\u75ab\u6d3b\u6027\u3092\u4fdd\u6301\u3057\u3084\u3059\u304f\u3001\u307e\u305f\u30a2\u30eb\u30c7\u30d2\u30c9\u57fa\u3092\u6301\u305f\u306a\u3044\u305f\u3081\u67b6\u6a4b\u53cd\u5fdc\u5f8c\u306e\u30d6\u30ed\u30c3\u30af\u51e6\u7406\u304c\u4e0d\u8981\u3068\u3044\u3046\u5229\u70b9\u304c\u3042\u308a\u307e\u3059\u3002<\/strong><\/p>\n<h2>\u6a19\u7684\u6297\u539f\u3068\u56fa\u5b9a\u6db2<\/h2>\n<table>\n<tbody>\n<tr>\n<td style=\"text-align: center\"><strong>\u6297\u539f<\/strong><\/td>\n<td style=\"text-align: center\"><strong>\u56fa\u5b9a\u6db2<\/strong><\/td>\n<\/tr>\n<tr>\n<td rowspan=\"3\">\u3055\u307e\u3056\u307e\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u3001\u30da\u30d7\u30c1\u30c9\u3001\u4f4e\u5206\u5b50\u91cf\u306e\u9175\u7d20<\/td>\n<td>4% Paraformaldehyde<\/td>\n<\/tr>\n<tr>\n<td>4% Paraformaldehyde-1% Glutaraldehyde<\/td>\n<\/tr>\n<tr>\n<td>10% Neutral-Buffered Formalin (NBF)<\/td>\n<\/tr>\n<tr>\n<td>\u8edf\u7d44\u7e54<\/td>\n<td>Bouin\u56fa\u5b9a\u6db2<\/td>\n<\/tr>\n<tr>\n<td>\u30a2\u30df\u30ce\u9178<\/td>\n<td>4% Paraformaldehyde-1% Glutaraldehyde<\/td>\n<\/tr>\n<tr>\n<td>\u6838\u9178<\/td>\n<td>Carnoy\u56fa\u5b9a\u6db2<\/td>\n<\/tr>\n<tr>\n<td>\u9ad8\u5206\u5b50\u91cf\u30bf\u30f3\u30d1\u30af\u8cea\uff08\u514d\u75ab\u30b0\u30ed\u30d6\u30ea\u30f3\uff09<\/td>\n<td>\u6c37\u51b7\u30a2\u30bb\u30c8\u30f3\u3001\u30e1\u30bf\u30ce\u30fc\u30eb (100%)<\/td>\n<\/tr>\n<tr>\n<td>\u305d\u306e\u4ed6\uff08\u96fb\u5b50\u9855\u5fae\u93e1\uff09<\/td>\n<td>4% Paraformaldehyde-1% Glutaraldehyde<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<table>\n<tbody>\n<tr>\n<td>\u56fa\u5b9a\u6db2<\/td>\n<td>\u7d44\u6210<\/td>\n<td>\u30b3\u30e1\u30f3\u30c8<\/td>\n<\/tr>\n<tr>\n<td>4% Paraformaldehyde in 0.1M Phosphate Buffer<\/td>\n<td>\u00a0\u4e0b\u8a18\u3092\u6df7\u5408<\/p>\n<ul>\n<li style=\"list-style-type: none\">\n<ul>\n<li style=\"list-style-type: none\">\n<ul>\n<li>NaH<sub>2<\/sub>PO<sub>4<\/sub>, 3.2g<\/li>\n<li>Na<sub>2<\/sub>HPO<sub>4<\/sub>, 10.9g<\/li>\n<li>Distilled water, 1000mL<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>pH\u30927.4\u306b\u8abf\u88fd\u5f8c\u4e0b\u8a18\u3092\u6dfb\u52a0<\/p>\n<ul>\n<li style=\"list-style-type: none\">\n<ul>\n<li>Paraformaldehyde, 40g<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/td>\n<td>\u00a060\u2103\u3067\u64b9\u62cc\u3057\u306a\u304c\u30891N NaOH\u30921-2\u6ef4\u305a\u3064\u6dfb\u52a0\u3057\u3066\u3001\u30d1\u30e9\u30db\u30eb\u30e0\u30a2\u30eb\u30c7\u30d2\u30c9\u3092\u6eb6\u89e3\u3057\u3001\u5ba4\u6e29\u306b\u623b\u3057\u3066\u308d\u904e<\/td>\n<\/tr>\n<tr>\n<td>\u00a04% Paraformaldehyde-1% Glutaraldehyde in 0.1M Phosphate Buffer<\/td>\n<td>\u00a0Prepare 4% paraformaldehyde in 0.1M phosphate buffer\uff08\u4e0a\u8a18\uff09\u306bGlutaraldehyde, 20mL\u3092\u6dfb\u52a0<\/td>\n<td><\/td>\n<\/tr>\n<tr>\n<td>\u00a0Bouin\u56fa\u5b9a\u6db2<\/td>\n<td>\u00a0\u4e0b\u8a18\u3092\u6df7\u5408<\/p>\n<ul>\n<li>Saturated aqueous picric acid, 750mL<\/li>\n<li>40% formaldehyde, 250mL<\/li>\n<li>Glacial acetic acid, 50mL<\/li>\n<\/ul>\n<\/td>\n<td>\u00a0\u5ba4\u6e29\u4fdd\u5b58<\/td>\n<\/tr>\n<tr>\n<td>\u00a010% Neutral-Buffered Formalin<\/td>\n<td>\u00a0\u4e0b\u8a18\u3092\u6df7\u5408<\/p>\n<ul>\n<li style=\"list-style-type: none\">\n<ul>\n<li style=\"list-style-type: none\">\n<ul>\n<li>Na<sub>2<\/sub>HPO<sub>4<\/sub>, anhydrous, 6.5g<\/li>\n<li>NaH<sub>2<\/sub>PO<sub>4<\/sub>\u30fbH<sub>2<\/sub>0, 4g<\/li>\n<li>Distilled water, 900mL<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>pH\u30927.4\u306b\u8abf\u88fd\u5f8c\u4e0b\u8a18\u3092\u6dfb\u52a0<\/p>\n<ul>\n<li style=\"list-style-type: none\">\n<ul>\n<li>40% formaldehyde, 100mL<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/td>\n<td>\u00a04\u2103\u4fdd\u5b58<\/td>\n<\/tr>\n<tr>\n<td>\u00a0Carnoy\u56fa\u5b9a\u6db2<\/td>\n<td>\u00a0\u4e0b\u8a18\u3092\u6df7\u5408<\/p>\n<ul>\n<li style=\"list-style-type: none\">\n<ul>\n<li>Ethanol (absolute), 60mL<\/li>\n<li>Chloroform, 30mL<\/li>\n<li>Acetic acid, glacial, 10mL<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/td>\n<td><\/td>\n<\/tr>\n<tr>\n<td>\u00a0\u6c88\u6bbf\u5264<\/td>\n<td>\u00a0\u6c37\u51b7\u30a2\u30bb\u30c8\u30f3\u3001\u30e1\u30bf\u30ce\u30fc\u30eb (100%)<\/td>\n<td>\u00a0\u5ba4\u6e29\u30675 ~ 10\u5206\u3067\u56fa\u5b9a<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h2>\u3010\u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3011\u30bf\u30f3\u30d1\u30af\u8cea\u89e3\u6790\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u30cf\u30f3\u30c9\u30d6\u30c3\u30af<\/h2>\n<p>\u52b9\u7387\u7684\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u62bd\u51fa\u304b\u3089\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u89e3\u6790\u30c4\u30fc\u30eb\u307e\u3067\u3001\u5305\u62ec\u7684\u306b\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u3092\u7d39\u4ecb\u3057\u3066\u304a\u308a\u307e\u3059\u3002PDF\u30d5\u30a1\u30a4\u30eb\u306e\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3092\u3054\u5e0c\u671b\u306e\u65b9\u306f\u3001\u4e0b\u8a18\u30dc\u30bf\u30f3\u3088\u308a\u304a\u7533\u8fbc\u307f\u304f\u3060\u3055\u3044\u3002<\/p>\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/catalogs-protein-form.html?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa14_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9<\/a><\/p>\n<h2>\u7d44\u7e54\u67d3\u8272\u95a2\u9023\u3001\u305d\u306e\u4ed6\u306e\u8a18\u4e8b\u306f\u3053\u3061\u3089<\/h2>\n<table style=\"border-color: lightgray;border-collapse: collapse;padding: 20px\" border=\"1\">\n<tbody>\n<tr>\n<td style=\"border-color: lightgray;padding: 20px\">\n<ul>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic17\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa14_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u7b2c17\u56de\u3011\u3088\u304f\u308f\u304b\u308b\u7d44\u7e54\u67d3\u8272\u306e\u57fa\u790e\u77e5\u8b58<br \/>\n<\/a><\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u7528\u306b\u306f\u4f7f\u7528\u3044\u305f\u3060\u3051\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u524d\u56de\u306f\u3001\u514d\u75ab\u7d44\u7e54\u5316\u5b66\uff08Immunohistochemistry; IHC\uff09\u306e\u6982\u8981\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3057\u305f\u3002\u4eca\u56de\u306f\u514d\u75ab\u7d44\u7e54\u5316\u5b66\u306b\u304a\u3044\u3066\u91cd\u8981\u306a\u30b9\u30c6\u30c3\u30d7\u3067\u3042\u308b\u56fa\u5b9a\u5316\u306b\u3064\u3044\u3066\u3082\u3046\u5c11\u3057\u8a73\u3057\u3044\u5185\u5bb9\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\uff01<\/p>\n","protected":false},"author":988,"featured_media":2333,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[159],"tags":[237,191],"division":[],"class_list":{"0":"post-213","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-cell","8":"tag-protein-experiments","9":"tag-immunostaining","10":"entry"},"_selected_authors":[],"_selected_reviewers":[],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.8 (Yoast SEO v27.8) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>\u7d44\u7e54\u67d3\u8272\u306b\u304a\u3051\u308b\u56fa\u5b9a\u5316\u307e\u3068\u3081\uff5c\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\uff01\u30bf\u30f3\u30d1\u30af\u8cea\u5b9f\u9a13\u3042\u308c\u3053\u308c \u7b2c18\u56de - Learning at the Bench<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic18\/\" \/>\n<meta property=\"og:locale\" content=\"ja_JP\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"\u7d44\u7e54\u67d3\u8272\u306b\u304a\u3051\u308b\u56fa\u5b9a\u5316\u307e\u3068\u3081\uff5c\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\uff01\u30bf\u30f3\u30d1\u30af\u8cea\u5b9f\u9a13\u3042\u308c\u3053\u308c 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