{"id":226,"date":"2020-09-16T00:00:00","date_gmt":"2020-09-15T15:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic19\/"},"modified":"2025-10-16T18:22:53","modified_gmt":"2025-10-16T09:22:53","slug":"qpcr-basic19","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic19\/","title":{"rendered":"\u3082\u3063\u3068\u30d7\u30e9\u30a4\u30de\u30fc\u8a2d\u8a08\u306e\u624b\u9593\u3092\u7701\u304d\u305f\u3044! \u301cTaqMan\u00ae Gene Expression Assays\uff5c\u4eca\u3053\u305d\u672c\u6c17\u3067\u5fb9\u5e95\u7406\u89e3! \u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u8b1b\u5ea7 \u7b2c19\u56de"},"content":{"rendered":"\n

\u4eca\u56de\u3001\u767b\u5834\u3059\u308b\u306e\u306f\u3001\u300cTaqMan\u00ae Gene Expression Assays<\/a>\u300d\u3067\u3059\u3002\u3082\u306f\u3084\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u8a2d\u8a08\u3084\u6761\u4ef6\u691c\u8a0e\u306f\u5fc5\u8981\u306a\u3044<\/strong>\u3001\u3068\u3044\u3046\u306e\u304c\u3053\u306e\u88fd\u54c1\u306e\u7279\u5fb4\u3067\u3059\u3002\u524d\u56de\u306b\u3054\u7d39\u4ecb\u3057\u305f\u300cPrimer Express\u00ae\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2<\/a>\u300d\u3082\u975e\u5e38\u306b\u7c21\u4fbf\u306a\u30c4\u30fc\u30eb\u3067\u3059\u304c\u3001\u5f0a\u793e\u306f\u3055\u3089\u306b\u30d7\u30e9\u30a4\u30de\u30fc\u8a2d\u8a08\u306e\u624b\u9593\u3092\u7701\u304f\u3053\u3068\u306e\u3067\u304d\u308b\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u3092\u958b\u767a\u3057\u307e\u3057\u305f\u3002<\/p>\n\n\n\n

\u524d\u56de\u306e\u30d6\u30ed\u30b0\uff1aTaqMan\u00ae\u304bSYBR\u00ae Green\u304b\u3067\u8ff7\u308f\u306a\u3044\uff5c\u4eca\u3053\u305d\u672c\u6c17\u3067\u5fb9\u5e95\u7406\u89e3! \u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u8b1b\u5ea7 \u7b2c18\u56de<\/a><\/p>\n\n\n\n

TaqMan\u00ae Gene Expression Assays\u3001\u4f55\u304c\u3067\u304d\u308b\u306e\uff1f<\/h2>\n\n\n\n

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TaqMan\u00ae Gene Expression Assays\u3001\u5b88\u5099\u7bc4\u56f2\u306f\uff1f<\/h2>\n\n\n\n

\u30d2\u30c8\u3001\u30de\u30a6\u30b9\u3001\u30e9\u30c3\u30c8\u306b\u52a0\u3048\u3066\u30b7\u30ed\u30a4\u30cc\u30ca\u30ba\u30ca\u3068\u30b7\u30e7\u30a6\u30b8\u30e7\u30a6\u30d0\u30a8\u3082\u52a0\u308f\u308a\u3001\u591a\u304f\u306e\u30e2\u30c7\u30eb\u751f\u7269\u3092\u5bfe\u8c61\u3068\u3057\u3066\u7dcf\u6570280\u4e07\u7a2e\u4ee5\u4e0a<\/strong>\u3082\u306eTaqMan\u00ae\u30a2\u30c3\u30bb\u30a4\u3092\u3054\u7528\u610f\u3057\u3066\u3044\u307e\u3059\u3002\u3082\u3061\u308d\u3093\u5168\u3066\u306e\u30a2\u30c3\u30bb\u30a4\u306f\u3001\u540c\u4e00\u306ePCR\u6761\u4ef6\u3067\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002<\/strong>\u516c\u7684\u306a\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9\u306e\u60c5\u5831\u306f\u3082\u3061\u308d\u3093\u3001Celera\u793e\u306e\u30aa\u30ea\u30b8\u30ca\u30eb\u30c7\u30fc\u30bf\u3092\u5229\u7528\u3057\u3066\u8a2d\u8a08\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u3055\u3089\u306b\u907a\u4f1d\u5b50\u767a\u73fe\u6291\u5236\u5b9f\u9a13\u306b\u7528\u3044\u308bsiRNA\u8a66\u85ac\u3068\u3082\u30ea\u30f3\u30af\u3057\u3066\u304a\u308a\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u907a\u4f1d\u5b50\u3092\u30ce\u30c3\u30af\u30c0\u30a6\u30f3\u3057\u305f\u3044\u5834\u5408\u306b\u3082\u3001\u76ee\u7684\u306esiRNA\u3068\u767a\u73fe\u5b9a\u91cf\u304c\u53ef\u80fd\u306a\u30a2\u30c3\u30bb\u30a4\u3092\u7c21\u5358\u306b\u691c\u7d22\u3067\u304d\u308b\u306e\u3067\u3059\u3002<\/p>\n\n\n\n

\u6700\u8fd1\u3067\u306f\u5404\u30e1\u30fc\u30ab\u30fc\u304b\u3089\u8a2d\u8a08\u6e08\u307f\u30ad\u30c3\u30c8\u304c\u8ca9\u58f2\u3055\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u30e9\u30a4\u30f3\u30ca\u30c3\u30d7\u306b\u5236\u9650\u304c\u3042\u308b\u30b1\u30fc\u30b9\u3084\u3001\u6a5f\u7a2e\u3054\u3068\u306bPCR\u6761\u4ef6\u304c\u7570\u306a\u308b\u306a\u3069\u3001\u305d\u306e\u4f7f\u3044\u3084\u3059\u3055\u3068\u4fe1\u983c\u6027\u306b\u306f\u82e5\u5e72\u306e\u4e0d\u5b89\u304c\u6b8b\u308a\u307e\u3059\u3002\u307e\u305f\u3001\u30b9\u30d7\u30e9\u30a4\u30b9\u30d0\u30ea\u30a2\u30f3\u30c8\u304c\u8003\u616e\u3055\u308c\u3066\u3044\u306a\u3044\u5834\u5408\u3084\u3001PCR\u30d7\u30e9\u30a4\u30de\u30fc\u306f\u81ea\u5206\u3067\u5225\u306b\u5408\u6210\u3057\u306a\u304f\u3066\u306f\u306a\u3089\u306a\u3044\u88fd\u54c1\u3082\u898b\u53d7\u3051\u3089\u308c\u307e\u3059\u3002\u305d\u306e\u70b9\u3001\u5f0a\u793e\u306e\u907a\u4f1d\u5b50\u767a\u73fe\u89e3\u6790\u7528\u306e\u30a2\u30c3\u30bb\u30a4\u306f\u3001\u958b\u767a\u5f53\u521d\u304b\u3089\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u30b7\u30b9\u30c6\u30e0\u3068\u306e\u6574\u5408\u6027\u3092\u8003\u616e\u3057\u3066\u30c7\u30b6\u30a4\u30f3\u3055\u308c\u3066\u3044\u307e\u3059\u306e\u3067\u3001\u5b89\u5fc3\u3057\u3066\u304a\u4f7f\u3044\u3044\u305f\u3060\u3051\u307e\u3059\u3002<\/p>\n\n\n\n

\u3053\u308c\u3089\u306e\u88fd\u54c1\u7fa4\u3092\u3046\u307e\u304f\u7d44\u307f\u5408\u308f\u305b\u308c\u3070\u3001\u307e\u3055\u306b\u30b7\u30fc\u30e0\u30ec\u30b9\u306a\u5b9f\u9a13\u74b0\u5883\u304c\u69cb\u7bc9\u3067\u304d\u308b\u306e\u3067\u3059\u306d\u3002\u6b21\u56de\u306f\u3001\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306b\u3064\u3044\u3066\u8003\u3048\u307e\u3059\u3002<\/p>\n\n\n\n

\n

\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u30cf\u30f3\u30c9\u30d6\u30c3\u30af \u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9<\/h4>\n\n\n\n

\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u7406\u8ad6\u3084\u5b9f\u9a13\u30c7\u30b6\u30a4\u30f3\u306e\u8a2d\u8a08\u306a\u3069\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u57fa\u790e\u77e5\u8b58\u304c\u63b2\u8f09\u3055\u308c\u305f\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3092\u59cb\u3081\u305f\u3070\u304b\u308a\u306e\u65b9\u3084\u3053\u308c\u304b\u3089\u5b9f\u9a13\u3092\u8003\u3048\u3066\u3044\u308b\u65b9\u306b\u3046\u3063\u3066\u3064\u3051\u306e\u30cf\u30f3\u30c9\u30d6\u30c3\u30af\u3067\u3059\u3002PDF\u30d5\u30a1\u30a4\u30eb\u306e\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3092\u3054\u5e0c\u671b\u306e\u65b9\u306f\u3001\u4e0b\u8a18\u30dc\u30bf\u30f3\u3088\u308a\u304a\u7533\u3057\u8fbc\u307f\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n

\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/p>\n<\/div>\n\n\n\n

<\/div>\n\n\n\n
\n

\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u30c8\u30e9\u30d6\u30eb\u30b7\u30e5\u30fc\u30c6\u30a3\u30f3\u30b0 \u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9<\/h4>\n\n\n\n

\u7570\u5e38\u306aS\u5b57\u72b6\u306e\u5897\u5e45\u66f2\u7dda\u3001NTC\u3067\u306e\u5897\u5e45\u691c\u51fa\u3001\u5897\u5e45\u304c\u898b\u3089\u308c\u306a\u3044\u306a\u3069\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u5b9f\u9a13\u4e2d\u306b\u56f0\u3063\u305f\u969b\u306e\u624b\u52a9\u3051\u306b\u306a\u308b\u60c5\u5831\u3092\u7d39\u4ecb\u3057\u3066\u3044\u307e\u3059\u3002PDF\u30d5\u30a1\u30a4\u30eb\u306e\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3092\u3054\u5e0c\u671b\u306e\u65b9\u306f\u3001\u4e0b\u8a18\u30dc\u30bf\u30f3\u3088\u308a\u304a\u7533\u3057\u8fbc\u307f\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n

\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/p>\n<\/div>\n\n\n\n

<\/div>\n\n\n\n
\n

TaqMan Gene Expression\u30a2\u30c3\u30bb\u30a4\u5c0e\u5165\u30ac\u30a4\u30c9 \u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9<\/h4>\n\n\n\n

TaqMan\u30a2\u30c3\u30bb\u30a4\u3092\u3053\u308c\u304b\u3089\u4f7f\u3046\u65b9\u3001\u4f7f\u3063\u3066\u3044\u308b\u3051\u3069\u3082\u3063\u3068\u3088\u304f\u77e5\u308a\u305f\u3044\u65b9\u306b\u3046\u3063\u3066\u3064\u3051\u306e\u30cf\u30f3\u30c9\u30d6\u30c3\u30af\u3067\u3059\u3002PDF\u30d5\u30a1\u30a4\u30eb\u306e\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3092\u3054\u5e0c\u671b\u306e\u65b9\u306f\u3001\u4e0b\u8a18\u30dc\u30bf\u30f3\u3088\u308a\u304a\u7533\u3057\u8fbc\u307f\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n

\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/p>\n<\/div>\n\n\n\n

<\/div>\n\n\n\n

\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u7528\u306b\u306f\u4f7f\u7528\u3044\u305f\u3060\u3051\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

\u3082\u306f\u3084\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u8a2d\u8a08\u3084\u6761\u4ef6\u691c\u8a0e\u306f\u5fc5\u8981\u306a\u3044!? \u30c7\u30b6\u30a4\u30f3\u6e08\u307f\u306e\u30ec\u30c7\u30a3\u30fc\u30e1\u30a4\u30c9\u30a2\u30c3\u30bb\u30a4\u300cTaqMan\u00ae Gene Expression 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