{"id":22896,"date":"2025-09-05T08:33:56","date_gmt":"2025-09-04T23:33:56","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/?p=22896"},"modified":"2025-09-17T14:18:00","modified_gmt":"2025-09-17T05:18:00","slug":"sanger-seq-ce-gsd-ts-ce-25054","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/sanger-seq-ce-gsd-ts-ce-25054\/","title":{"rendered":"\u65b0\u3057\u3044\u30d9\u30fc\u30b9\u30b3\u30fc\u30e9\u30fc\u3092\u6d3b\u7528\u3057\u305f\u3001Indel\u3092\u542b\u3080\u30b7\u30fc\u30b1\u30f3\u30b9\u89e3\u6790\u306e\u52b9\u7387\u5316"},"content":{"rendered":"\n<p>\u30c0\u30a4\u30ec\u30af\u30c8\u30b7\u30fc\u30b1\u30f3\u30b9\u6cd5\u3068\u306f\u3001\u5927\u8178\u83cc\u3092\u7528\u3044\u305f\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u306a\u3069\u306e\u30b9\u30c6\u30c3\u30d7\u3092\u4ecb\u3055\u305a\u3001PCR\u7523\u7269\u3092\u76f4\u63a5\u92f3\u578b\u3068\u3057\u3066\u5869\u57fa\u914d\u5217\u3092\u6c7a\u5b9a\u3059\u308b\u65b9\u6cd5\u3067\u3059\u3002\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3059\u308b\u65b9\u6cd5\u306b\u6bd4\u3079\u3001\u77ed\u6642\u9593\u3067\u7c21\u4fbf\u306b\u30b7\u30fc\u30b1\u30f3\u30b9\u89e3\u6790\u7d50\u679c\u3092\u53d6\u5f97\u3067\u304d\u308b\u305f\u3081\u3001\u5e83\u304f\u5229\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u4e00\u65b9\u3001\u30d8\u30c6\u30ed\u63a5\u5408\u3067\u633f\u5165\u3084\u6b20\u640d\uff08Indel\uff09\u3092\u542b\u3080\u30b5\u30f3\u30d7\u30eb\u3092\u30c0\u30a4\u30ec\u30af\u30c8\u30b7\u30fc\u30b1\u30f3\u30b9\u6cd5\u3067\u89e3\u6790\u3057\u305f\u5834\u5408\u3001Indel\u4ee5\u964d\u304c\u4e8c\u91cd\u6ce2\u5f62\u306b\u306a\u308a\u3001\u5869\u57fa\u914d\u5217\u306e\u89e3\u8aad\u304c\u56f0\u96e3\u306b\u306a\u308b\u8ab2\u984c\u304c\u3042\u308a\u307e\u3059\u3002\u672c\u30d6\u30ed\u30b0\u3067\u306f\u3001\u3053\u306e\u8ab2\u984c\u3092\u89e3\u6d88\u3059\u308b\u305f\u3081\u306e\u30a2\u30d7\u30ed\u30fc\u30c1\u3092\u3054\u7d39\u4ecb\u3044\u305f\u3057\u307e\u3059\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">\u65b0\u3057\u3044\u30d9\u30fc\u30b9\u30b3\u30fc\u30e9\u30fc\u201cSDB\u201d<\/h2>\n\n\n\n<p>\u30b8\u30a7\u30cd\u30c6\u30a3\u30c3\u30af\u30a2\u30ca\u30e9\u30a4\u30b6\u3067\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u7523\u7269\u3092\u6cf3\u52d5\u3057\u3001\u53d6\u5f97\u3055\u308c\u305f\u86cd\u5149\u30b7\u30b0\u30ca\u30eb\u306e\u60c5\u5831\u306f\u3001\u30d9\u30fc\u30b9\u30b3\u30fc\u30e9\u30fc\u3068\u547c\u3070\u308c\u308b\u30a2\u30eb\u30b4\u30ea\u30ba\u30e0\u3067\u89e3\u6790\u3059\u308b\u3053\u3068\u3067\u3001\u5869\u57fa\u914d\u5217\u306e\u60c5\u5831\u306b\u5909\u63db\u3055\u308c\u307e\u3059\u3002\u5f53\u793e\u304c\u63d0\u4f9b\u3059\u308b\u30b7\u30fc\u30b1\u30f3\u30b9\u89e3\u6790\u30bd\u30d5\u30c8\u30a6\u30a8\u30a2\u306e\u591a\u304f\u306f\u3001Applied Biosystems\u2122 KB\u2122 Basecaller\u3092\u63a1\u7528\u3057\u3066\u3044\u307e\u3059\u304c\u3001\u65b0\u3057\u3044\u30d9\u30fc\u30b9\u30b3\u30fc\u30e9\u30fc\u3068\u3057\u3066\u3001<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/sequencing\/sanger-sequencing\/sanger-dna-sequencing\/sanger-sequencing-data-analysis\/smart-deep-basecaller.html?CID=gsd_pcr_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_sanger_seq_ce_gsd_ts_ce_25054_Social_LAB\" target=\"_blank\" rel=\"noopener\">Applied Biosystems\u2122 Smart Deep\u2122 Basecaller<\/a>\uff08SDB\uff09\u3092\u3054\u5229\u7528\u3044\u305f\u3060\u3051\u308b\u3088\u3046\u306b\u306a\u308a\u307e\u3057\u305f\u3002SDB\u306f\u3001<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/A38938?SID=srch-srp-A38938&amp;CID=gsd_pcr_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_sanger_seq_ce_gsd_ts_ce_25054_Social_LAB\" target=\"_blank\" rel=\"noreferrer noopener\">Applied Biosystems\u2122 Sequencing Analysis\u30bd\u30d5\u30c8\u30a6\u30a8\u30a2 v8<\/a>\u3067\u4f7f\u7528\u3067\u304d\u307e\u3059<sup>\u203b1<\/sup>\u3002<br>\u203b1\u3000SDB\u306e\u4f7f\u7528\u306b\u306f\u6709\u6599\u306e\u5e74\u9593\u30e9\u30a4\u30bb\u30f3\u30b9\u306e\u53d6\u5f97\u304c\u5fc5\u8981\u3067\u3059\u3002<\/p>\n\n\n\n<p>KB Basecaller\u3092\u7528\u3044\u305f\u89e3\u6790\u3067\u306f\u3001\u30d8\u30c6\u30ed\u63a5\u5408\u6027Indel\u3092\u542b\u3080\u5869\u57fa\u914d\u5217\u3092\u6b63\u3057\u304f\u89e3\u6790\u3067\u304d\u306a\u3044\u8ab2\u984c\u304c\u3042\u308a\u307e\u3057\u305f\u304c\u3001SDB\u3067\u306f\u3001\u3088\u308a\u9ad8\u7cbe\u5ea6\u306e\u30d9\u30fc\u30b9\u30b3\u30fc\u30eb\u304c\u884c\u3048\u307e\u3059\u3002Sequencing Analysis\u30bd\u30d5\u30c8\u30a6\u30a8\u30a2 v8\u3092\u7528\u3044\u3001\u30d8\u30c6\u30ed\u63a5\u5408\u6027Indel\u306e\u30b5\u30f3\u30d7\u30eb\u3092\u3001KB Basecaller\u3068SDB\u3067\u89e3\u6790\u3057\u305f\u7d50\u679c\u3092\u56f31\u306b\u793a\u3057\u307e\u3059\u3002\u8d64\u77e2\u5370\u3067\u793a\u3059\u7b87\u6240\u304b\u3089\u6ce2\u5f62\u304c\u4e8c\u91cd\u306b\u306a\u3063\u3066\u304a\u308a\u3001\u3053\u306e\u7b87\u6240\u306bIndel\u304c\u5b58\u5728\u3059\u308b\u3053\u3068\u304c\u308f\u304b\u308a\u307e\u3059\u3002\u30a8\u30ec\u30af\u30c8\u30ed\u30d5\u30a7\u30ed\u30b0\u30e9\u30e0\u306e\u5404\u30d4\u30fc\u30af\u306e\u4e0a\u90e8\u306b\u306f\u3001\u30d4\u30fc\u30af\u60c5\u5831\u306b\u57fa\u3065\u3044\u3066\u89e3\u6790\uff08\u30d9\u30fc\u30b9\u30b3\u30fc\u30eb\uff09\u3055\u308c\u305f\u5869\u57fa\u304c\u8868\u793a\u3055\u308c\u307e\u3059\u3002\u56f31\u306f\u3001\u540c\u4e00\u7b87\u6240\u306b2\u3064\u306e\u30d4\u30fc\u30af\u304c\u691c\u51fa\u3055\u308c\u305f\u5834\u5408\u3001\u6df7\u5408\u5869\u57fa\uff08A\u3068G\u306fR\u3001C\u3068T\u306fY\u3001\u306a\u3069\uff09\u3068\u8868\u793a\u3059\u308b\u8a2d\u5b9a\u3067\u89e3\u6790\u3057\u305f\u7d50\u679c\u3067\u3059\u3002Sequencing Analysis\u30bd\u30d5\u30c8\u30a6\u30a8\u30a2\u3067\u306f\u3001\u5869\u57fa\u60c5\u5831\u306fIUPAC\/IUB\u30b3\u30fc\u30c9\u306b\u306e\u3063\u3068\u3063\u3066\u8868\u793a\u3055\u308c\u307e\u3059\u3002KB Basecaller\u3067\u89e3\u6790\u3057\u305f\u7d50\u679c\u3067\u306f\u3001\u30d4\u30fc\u30af\u304c\u91cd\u306a\u3063\u3066\u3044\u308b\u306b\u3082\u95a2\u308f\u3089\u305a\u3001\u5358\u4e00\u5869\u57fa\u8868\u793a\uff08A\u3001T\u3001G\u3001\u307e\u305f\u306fC\uff09\u3055\u308c\u3066\u3044\u308b\u30a8\u30e9\u30fc\u7b87\u6240\u304c\u8907\u6570\u898b\u3089\u308c\u307e\u3059\uff08\u56f31\u3000\u8d64\u67a0\u90e8\uff09\u3002\u3053\u308c\u306b\u5bfe\u3057\u3001SDB\u3067\u89e3\u6790\u3057\u305f\u7d50\u679c\u306f\u3001\u30d4\u30fc\u30af\u304c\u91cd\u306a\u3063\u3066\u3044\u308b\u7b87\u6240\u304c\u6df7\u5408\u5869\u57fa\u3068\u3057\u3066\u6b63\u3057\u304f\u30d9\u30fc\u30b9\u30b3\u30fc\u30eb\u3055\u308c\u307e\u3057\u305f\u3002<\/p>\n\n\n\n<figure data-wp-context=\"{&quot;imageId&quot;:&quot;6adea9e6a45b1&quot;}\" data-wp-interactive=\"core\/image\" data-wp-key=\"6adea9e6a45b1\" class=\"wp-block-image aligncenter size-large wp-lightbox-container\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"720\" data-wp-class--hide=\"state.isContentHidden\" data-wp-class--show=\"state.isContentVisible\" data-wp-init=\"callbacks.setButtonStyles\" data-wp-on--click=\"actions.showLightbox\" data-wp-on--load=\"callbacks.setButtonStyles\" data-wp-on-window--resize=\"callbacks.setButtonStyles\" src=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/08\/sanger-seq-ce-gsd-ts-ce-25054_image_01-1024x720.jpg\" alt=\"\u30d8\u30c6\u30ed\u63a5\u5408\u6027Indel\u30b5\u30f3\u30d7\u30eb\u306e\u30b7\u30fc\u30b1\u30f3\u30b9\u89e3\u6790\u7d50\u679c\" class=\"wp-image-22898\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/08\/sanger-seq-ce-gsd-ts-ce-25054_image_01-1024x720.jpg 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/08\/sanger-seq-ce-gsd-ts-ce-25054_image_01-300x211.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/08\/sanger-seq-ce-gsd-ts-ce-25054_image_01-768x540.jpg 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/08\/sanger-seq-ce-gsd-ts-ce-25054_image_01-1536x1080.jpg 1536w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/08\/sanger-seq-ce-gsd-ts-ce-25054_image_01-2048x1439.jpg 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><button\n\t\t\tclass=\"lightbox-trigger\"\n\t\t\ttype=\"button\"\n\t\t\taria-haspopup=\"dialog\"\n\t\t\taria-label=\"Enlarge\"\n\t\t\tdata-wp-init=\"callbacks.initTriggerButton\"\n\t\t\tdata-wp-on--click=\"actions.showLightbox\"\n\t\t\tdata-wp-style--right=\"state.imageButtonRight\"\n\t\t\tdata-wp-style--top=\"state.imageButtonTop\"\n\t\t>\n\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"12\" height=\"12\" fill=\"none\" viewBox=\"0 0 12 12\">\n\t\t\t\t<path fill=\"#fff\" d=\"M2 0a2 2 0 0 0-2 2v2h1.5V2a.5.5 0 0 1 .5-.5h2V0H2Zm2 10.5H2a.5.5 0 0 1-.5-.5V8H0v2a2 2 0 0 0 2 2h2v-1.5ZM8 12v-1.5h2a.5.5 0 0 0 .5-.5V8H12v2a2 2 0 0 1-2 2H8Zm2-12a2 2 0 0 1 2 2v2h-1.5V2a.5.5 0 0 0-.5-.5H8V0h2Z\" \/>\n\t\t\t<\/svg>\n\t\t<\/button><\/figure>\n\n\n\n<p class=\"has-text-align-center\"><strong>\u56f31. \u30d8\u30c6\u30ed\u63a5\u5408\u6027Indel\u30b5\u30f3\u30d7\u30eb\u306e\u30b7\u30fc\u30b1\u30f3\u30b9\u89e3\u6790\u7d50\u679c<\/strong><br>a) KB Basecaller\u3001b) SDB\u3092\u7528\u3044\u30d9\u30fc\u30b9\u30b3\u30fc\u30eb\u3092\u5b9f\u884c\u3002\u6df7\u5408\u5869\u57fa\u3068\u3057\u3066\u30d9\u30fc\u30b9\u30b3\u30fc\u30eb\u3055\u308c\u305f\u7b87\u6240\u3092\u3001\u9ec4\u8272\u3067\u30cf\u30a4\u30e9\u30a4\u30c8\u8868\u793a\u3002\u3000\u8d64\u77e2\u5370\uff1aIndel\u304c\u5b58\u5728\u3059\u308b\u3068\u63a8\u5b9a\u3055\u308c\u308b\u7b87\u6240\u3002 \u8d64\u67a0\uff1a\u30d9\u30fc\u30b9\u30b3\u30fc\u30eb\u30a8\u30e9\u30fc\u7b87\u6240\u3002<\/p>\n\n\n\n<p>\u3053\u306e\u7d50\u679c\u304b\u3089\u3001SDB\u304c\u3088\u308a\u7cbe\u5ea6\u306e\u9ad8\u3044\u30d9\u30fc\u30b9\u30b3\u30fc\u30e9\u30fc\u3067\u3042\u308b\u3053\u3068\u3092\u3054\u7406\u89e3\u3044\u305f\u3060\u3051\u308b\u3068\u601d\u3044\u307e\u3059\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u6df7\u5408\u5869\u57fa\u8868\u793a\u3055\u308c\u305f\u5869\u57fa\u914d\u5217\u304b\u3089\u306eindel\u306e\u5224\u5b9a\">\u6df7\u5408\u5869\u57fa\u8868\u793a\u3055\u308c\u305f\u5869\u57fa\u914d\u5217\u304b\u3089\u306eIndel\u306e\u5224\u5b9a<\/h2>\n\n\n\n<p>\u56f31\u306b\u793a\u3059\u3088\u3046\u306b\u3001\u30d8\u30c6\u30ed\u63a5\u5408\u6027Indel\u30b5\u30f3\u30d7\u30eb\u306f\u3001Indel\u4ee5\u964d\u306e\u6ce2\u5f62\u304c\u4e8c\u91cd\u306b\u306a\u308b\u305f\u3081\u3001\u5869\u57fa\u914d\u5217\u306e\u89e3\u8aad\u304c\u56f0\u96e3\u3067\u3059\u3002SDB\u3067\u30d9\u30fc\u30b9\u30b3\u30fc\u30eb\u3055\u308c\u305f\u5869\u57fa\u914d\u5217\u3092\u6d3b\u7528\u3059\u308b\u3053\u3068\u3067\u3001\u3053\u306e\u8ab2\u984c\u3092\u89e3\u6c7a\u3067\u304d\u308b\u306e\u3067\u306f\u306a\u3044\u304b\u3068\u8003\u3048\u307e\u3057\u305f\u3002<br>\u89e3\u6790\u65b9\u6cd5\u306e\u6982\u5ff5\u56f3\u3092\u56f32\u306b\u793a\u3057\u307e\u3059\u3002SDB\u3067\u306f\u3001Indel\u4ee5\u964d\u306e\u4e8c\u91cd\u6ce2\u5f62\u306e\u9818\u57df\u3092\u3001\u6b63\u78ba\u306b\u6df7\u5408\u5869\u57fa\u3068\u3057\u3066\u30d9\u30fc\u30b9\u30b3\u30fc\u30eb\u3067\u304d\u307e\u3059\u3002\u3053\u306e\u89e3\u6790\u65b9\u6cd5\u3067\u306f\u307e\u305a\u3001SDB\u3067\u30d9\u30fc\u30b9\u30b3\u30fc\u30eb\u3055\u308c\u305f\u5869\u57fa\u914d\u5217\u4e2d\u306e\u6df7\u5408\u5869\u57fa\u3092\u3001\u5358\u4e00\u5869\u57fa\u306b\u5909\u63db\u3057\u307e\u3059\u3002\u6b21\u306b\u3001\u5358\u4e00\u5869\u57fa\u306b\u5909\u63db\u3055\u308c\u305f\u5869\u57fa\u306e\u60c5\u5831\u3092\u3001\u30ea\u30d5\u30a1\u30ec\u30f3\u30b9\u914d\u5217\u3068\u6bd4\u8f03\u3057\u3001\u30ea\u30d5\u30a1\u30ec\u30f3\u30b9\u3068\u7570\u306a\u308b\u5869\u57fa\u3092\u62bd\u51fa\u3057\u305f\u914d\u5217\u60c5\u5831\u3092\u53d6\u5f97\u3057\u307e\u3059\uff08\u56f32\u3000\u62bd\u51fa\u914d\u5217\uff09\u3002\u30ea\u30d5\u30a1\u30ec\u30f3\u30b9\u914d\u5217\u3068\u62bd\u51fa\u914d\u5217\u3092\u6bd4\u8f03\u3059\u308b\u3053\u3068\u3067\u3001Indel\u3092\u5224\u5b9a\u3067\u304d\u307e\u3059\u3002\u56f32\u306e\u5834\u5408\u3001\u30ea\u30d5\u30a1\u30ec\u30f3\u30b9\u914d\u5217\u306b\u306f\u5b58\u5728\u3059\u308bC\uff08\u8d64\u67a0\u90e8\uff09\u304c\u3001\u62bd\u51fa\u914d\u5217\u3067\u306f\u6b20\u640d\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u308f\u304b\u308a\u307e\u3059\u3002<\/p>\n\n\n\n<figure data-wp-context=\"{&quot;imageId&quot;:&quot;6adea9e6a4afe&quot;}\" data-wp-interactive=\"core\/image\" data-wp-key=\"6adea9e6a4afe\" class=\"wp-block-image aligncenter size-large wp-lightbox-container\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"357\" data-wp-class--hide=\"state.isContentHidden\" data-wp-class--show=\"state.isContentVisible\" data-wp-init=\"callbacks.setButtonStyles\" data-wp-on--click=\"actions.showLightbox\" data-wp-on--load=\"callbacks.setButtonStyles\" data-wp-on-window--resize=\"callbacks.setButtonStyles\" src=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/08\/sanger-seq-ce-gsd-ts-ce-25054_image_02-1024x357.jpg\" alt=\"Indel\u89e3\u6790\u65b9\u6cd5\u306e\u6982\u5ff5\u56f3\" class=\"wp-image-22900\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/08\/sanger-seq-ce-gsd-ts-ce-25054_image_02-1024x357.jpg 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/08\/sanger-seq-ce-gsd-ts-ce-25054_image_02-300x104.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/08\/sanger-seq-ce-gsd-ts-ce-25054_image_02-768x267.jpg 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/08\/sanger-seq-ce-gsd-ts-ce-25054_image_02-1536x535.jpg 1536w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/08\/sanger-seq-ce-gsd-ts-ce-25054_image_02-2048x713.jpg 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><button\n\t\t\tclass=\"lightbox-trigger\"\n\t\t\ttype=\"button\"\n\t\t\taria-haspopup=\"dialog\"\n\t\t\taria-label=\"Enlarge\"\n\t\t\tdata-wp-init=\"callbacks.initTriggerButton\"\n\t\t\tdata-wp-on--click=\"actions.showLightbox\"\n\t\t\tdata-wp-style--right=\"state.imageButtonRight\"\n\t\t\tdata-wp-style--top=\"state.imageButtonTop\"\n\t\t>\n\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"12\" height=\"12\" fill=\"none\" viewBox=\"0 0 12 12\">\n\t\t\t\t<path fill=\"#fff\" d=\"M2 0a2 2 0 0 0-2 2v2h1.5V2a.5.5 0 0 1 .5-.5h2V0H2Zm2 10.5H2a.5.5 0 0 1-.5-.5V8H0v2a2 2 0 0 0 2 2h2v-1.5ZM8 12v-1.5h2a.5.5 0 0 0 .5-.5V8H12v2a2 2 0 0 1-2 2H8Zm2-12a2 2 0 0 1 2 2v2h-1.5V2a.5.5 0 0 0-.5-.5H8V0h2Z\" \/>\n\t\t\t<\/svg>\n\t\t<\/button><\/figure>\n\n\n\n<p class=\"has-text-align-center\"><strong>\u56f32. Indel\u89e3\u6790\u65b9\u6cd5\u306e\u6982\u5ff5\u56f3<\/strong><\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u5c02\u7528\u30c4\u30fc\u30eb\u3092\u7528\u3044\u305findel\u89e3\u6790\u306e\u52b9\u7387\u5316\">\u5c02\u7528\u30c4\u30fc\u30eb\u3092\u7528\u3044\u305fIndel\u89e3\u6790\u306e\u52b9\u7387\u5316<\/h2>\n\n\n\n<p>\u4e0a\u8ff0\u3057\u305f\u65b9\u6cd5\u3067\u3001\u5b9f\u969b\u306b\u30d8\u30c6\u30ed\u63a5\u5408\u6027Indel\u30b5\u30f3\u30d7\u30eb\u3092\u89e3\u6790\u3067\u304d\u308b\u304b\u691c\u8a3c\u3059\u308b\u305f\u3081\u306b\u3001Applied Biosystems\u2122 3730xl DNA\u30a2\u30ca\u30e9\u30a4\u30b6\u3067\u53d6\u5f97\u3055\u308c\u305f\u30e9\u30f3\u30c7\u30fc\u30bf\u3092\u89e3\u6790\u3057\u307e\u3057\u305f\u3002<br>\u307e\u305a\u3001Sequencing Analysis\u30bd\u30d5\u30c8\u30a6\u30a8\u30a2 v8\u3092\u4f7f\u7528\u3057\u3001\u30e9\u30f3\u30c7\u30fc\u30bf\u3092SDB\u3067\u518d\u89e3\u6790\u3057\u307e\u3057\u305f\u3002\u518d\u89e3\u6790\u3057\u305f\u30a8\u30ec\u30af\u30c8\u30ed\u30d5\u30a7\u30ed\u30b0\u30e9\u30e0\u306e\u4e00\u90e8\u3092\u56f33\u306b\u793a\u3057\u307e\u3059\u3002a)\u306f\u30d8\u30c6\u30ed\u63a5\u5408\u6027Indel\u30b5\u30f3\u30d7\u30eb\u3001b)\u306fIndel\u3092\u542b\u307e\u306a\u3044\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u30b5\u30f3\u30d7\u30eb\u3067\u3059\u3002SDB\u3067\u518d\u89e3\u6790\u3057\u3066\u5f97\u3089\u308c\u305f\u5869\u57fa\u914d\u5217\u60c5\u5831\uff08\u88681\uff09\u306f\u3001\u30c6\u30ad\u30b9\u30c8\u30d5\u30a1\u30a4\u30eb\uff08.seq\u30d5\u30a1\u30a4\u30eb\uff09\u3068\u3057\u3066\u51fa\u529b\u3057\u307e\u3057\u305f\u3002<\/p>\n\n\n\n<figure data-wp-context=\"{&quot;imageId&quot;:&quot;6adea9e6a4f5b&quot;}\" data-wp-interactive=\"core\/image\" data-wp-key=\"6adea9e6a4f5b\" class=\"wp-block-image aligncenter size-large wp-lightbox-container\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"675\" data-wp-class--hide=\"state.isContentHidden\" data-wp-class--show=\"state.isContentVisible\" data-wp-init=\"callbacks.setButtonStyles\" data-wp-on--click=\"actions.showLightbox\" data-wp-on--load=\"callbacks.setButtonStyles\" data-wp-on-window--resize=\"callbacks.setButtonStyles\" src=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/08\/sanger-seq-ce-gsd-ts-ce-25054_image_03-1024x675.jpg\" alt=\"\u30b7\u30fc\u30b1\u30f3\u30b9\u89e3\u6790\u7d50\u679c\" class=\"wp-image-22901\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/08\/sanger-seq-ce-gsd-ts-ce-25054_image_03-1024x675.jpg 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/08\/sanger-seq-ce-gsd-ts-ce-25054_image_03-300x198.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/08\/sanger-seq-ce-gsd-ts-ce-25054_image_03-768x506.jpg 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/08\/sanger-seq-ce-gsd-ts-ce-25054_image_03-1536x1013.jpg 1536w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/08\/sanger-seq-ce-gsd-ts-ce-25054_image_03-2048x1350.jpg 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><button\n\t\t\tclass=\"lightbox-trigger\"\n\t\t\ttype=\"button\"\n\t\t\taria-haspopup=\"dialog\"\n\t\t\taria-label=\"Enlarge\"\n\t\t\tdata-wp-init=\"callbacks.initTriggerButton\"\n\t\t\tdata-wp-on--click=\"actions.showLightbox\"\n\t\t\tdata-wp-style--right=\"state.imageButtonRight\"\n\t\t\tdata-wp-style--top=\"state.imageButtonTop\"\n\t\t>\n\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"12\" height=\"12\" fill=\"none\" viewBox=\"0 0 12 12\">\n\t\t\t\t<path fill=\"#fff\" d=\"M2 0a2 2 0 0 0-2 2v2h1.5V2a.5.5 0 0 1 .5-.5h2V0H2Zm2 10.5H2a.5.5 0 0 1-.5-.5V8H0v2a2 2 0 0 0 2 2h2v-1.5ZM8 12v-1.5h2a.5.5 0 0 0 .5-.5V8H12v2a2 2 0 0 1-2 2H8Zm2-12a2 2 0 0 1 2 2v2h-1.5V2a.5.5 0 0 0-.5-.5H8V0h2Z\" \/>\n\t\t\t<\/svg>\n\t\t<\/button><\/figure>\n\n\n\n<p class=\"has-text-align-center\"><strong>\u56f33. \u30b7\u30fc\u30b1\u30f3\u30b9\u89e3\u6790\u7d50\u679c<\/strong><br>a)\u30d8\u30c6\u30ed\u63a5\u5408\u6027Indel\u30b5\u30f3\u30d7\u30eb\u3001b)\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u30b5\u30f3\u30d7\u30eb\u3002<br>\u6df7\u5408\u5869\u57fa\u3068\u3057\u3066\u30d9\u30fc\u30b9\u30b3\u30fc\u30eb\u3055\u308c\u305f\u7b87\u6240\u3092\u3001\u9ec4\u8272\u3067\u30cf\u30a4\u30e9\u30a4\u30c8\u8868\u793a\u3002<\/p>\n\n\n\n<p class=\"has-text-align-center\"><\/p>\n\n\n\n<div class=\"wp-block-group is-vertical is-layout-flex wp-container-core-group-is-layout-51fb755c wp-block-group-is-layout-flex\">\n<p><strong>\u88681. SDB\u3067\u30d9\u30fc\u30b9\u30b3\u30fc\u30eb\u3055\u308c\u305f\u5869\u57fa\u914d\u5217<\/strong><\/p>\n\n\n\n<figure data-wp-context=\"{&quot;imageId&quot;:&quot;6adea9e6a536c&quot;}\" data-wp-interactive=\"core\/image\" data-wp-key=\"6adea9e6a536c\" class=\"wp-block-image aligncenter size-large wp-lightbox-container\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"410\" data-wp-class--hide=\"state.isContentHidden\" data-wp-class--show=\"state.isContentVisible\" data-wp-init=\"callbacks.setButtonStyles\" data-wp-on--click=\"actions.showLightbox\" data-wp-on--load=\"callbacks.setButtonStyles\" data-wp-on-window--resize=\"callbacks.setButtonStyles\" src=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/08\/sanger-seq-ce-gsd-ts-ce-25054_table_01-1024x410.jpg\" alt=\"SDB\u3067\u30d9\u30fc\u30b9\u30b3\u30fc\u30eb\u3055\u308c\u305f\u5869\u57fa\u914d\u5217\" class=\"wp-image-22902\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/08\/sanger-seq-ce-gsd-ts-ce-25054_table_01-1024x410.jpg 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/08\/sanger-seq-ce-gsd-ts-ce-25054_table_01-300x120.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/08\/sanger-seq-ce-gsd-ts-ce-25054_table_01-768x308.jpg 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/08\/sanger-seq-ce-gsd-ts-ce-25054_table_01-1536x615.jpg 1536w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/08\/sanger-seq-ce-gsd-ts-ce-25054_table_01-2048x821.jpg 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><button\n\t\t\tclass=\"lightbox-trigger\"\n\t\t\ttype=\"button\"\n\t\t\taria-haspopup=\"dialog\"\n\t\t\taria-label=\"Enlarge\"\n\t\t\tdata-wp-init=\"callbacks.initTriggerButton\"\n\t\t\tdata-wp-on--click=\"actions.showLightbox\"\n\t\t\tdata-wp-style--right=\"state.imageButtonRight\"\n\t\t\tdata-wp-style--top=\"state.imageButtonTop\"\n\t\t>\n\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"12\" height=\"12\" fill=\"none\" viewBox=\"0 0 12 12\">\n\t\t\t\t<path fill=\"#fff\" d=\"M2 0a2 2 0 0 0-2 2v2h1.5V2a.5.5 0 0 1 .5-.5h2V0H2Zm2 10.5H2a.5.5 0 0 1-.5-.5V8H0v2a2 2 0 0 0 2 2h2v-1.5ZM8 12v-1.5h2a.5.5 0 0 0 .5-.5V8H12v2a2 2 0 0 1-2 2H8Zm2-12a2 2 0 0 1 2 2v2h-1.5V2a.5.5 0 0 0-.5-.5H8V0h2Z\" \/>\n\t\t\t<\/svg>\n\t\t<\/button><\/figure>\n<\/div>\n\n\n\n<p><\/p>\n\n\n\n<p>\u56f32\u306e\u300c\u6df7\u5408\u5869\u57fa\u304b\u3089\u5358\u4e00\u5869\u57fa\u3078\u306e\u5909\u63db\u300d\u3001\u300c\u30ea\u30d5\u30a1\u30ec\u30f3\u30b9\u914d\u5217\u3068\u6bd4\u8f03\u3057\u3001\u7570\u306a\u308b\u5869\u57fa\u3092\u62bd\u51fa\u300d\u306e\u5de5\u7a0b\u3092\u52b9\u7387\u5316\u3059\u308b\u305f\u3081\u306b\u3001Microsoft\u2122 Excel\u2122\u306e\u30de\u30af\u30ed\u3092\u4f7f\u7528\u3057\u3066\u30c4\u30fc\u30eb\u3092\u4f5c\u6210\u3057\u307e\u3057\u305f\u3002<br>\u4f5c\u6210\u3057\u305f\u30c4\u30fc\u30eb\u306b\u3001\u30d8\u30c6\u30ed\u63a5\u5408\u6027Indel\u30b5\u30f3\u30d7\u30eb\u306e.seq\u30d5\u30a1\u30a4\u30eb\u3092\u8aad\u307f\u8fbc\u3080\u3068\u3001\u81ea\u52d5\u7684\u306b\u3001\u6df7\u5408\u5869\u57fa\u304c\u5358\u4e00\u5869\u57fa\u306b\u5909\u63db\u3055\u308c\u307e\u3059\u3002\u6b21\u306b\u30ea\u30d5\u30a1\u30ec\u30f3\u30b9\u3068\u3057\u3066\u4f7f\u7528\u3059\u308b\u30d5\u30a1\u30a4\u30eb\u3092\u8aad\u307f\u8fbc\u307f\u307e\u3059\u3002\u4eca\u56de\u306f\u3001\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u30b5\u30f3\u30d7\u30eb\u3092\u30ea\u30d5\u30a1\u30ec\u30f3\u30b9\u3068\u3057\u3066\u7528\u3044\u307e\u3057\u305f\u3002\u30ea\u30d5\u30a1\u30ec\u30f3\u30b9\u30d5\u30a1\u30a4\u30eb\u3092\u8aad\u307f\u8fbc\u3080\u3068\u3001\u30d8\u30c6\u30ed\u63a5\u5408\u6027Indel\u30b5\u30f3\u30d7\u30eb\u3068\u30ea\u30d5\u30a1\u30ec\u30f3\u30b9\u306e\u5869\u57fa\u914d\u5217\u3092\u6bd4\u8f03\u3057\u3001\u62bd\u51fa\u914d\u5217\u304c\u81ea\u52d5\u7684\u306b\u4f5c\u6210\u3055\u308c\u307e\u3059\u3002\u30c4\u30fc\u30eb\u3067\u306e\u89e3\u6790\u7d50\u679c\u306e\u4e00\u90e8\u3092\u56f34\u306b\u793a\u3057\u307e\u3059\u3002\u30ea\u30d5\u30a1\u30ec\u30f3\u30b9\u3068\u62bd\u51fa\u914d\u5217\u3092\u6bd4\u8f03\u3059\u308b\u3053\u3068\u3067\u3001C\u306e\u6b20\u640d\u3068\u5224\u5b9a\u3067\u304d\u307e\u3057\u305f\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image aligncenter size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"186\" src=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/08\/sanger-seq-ce-gsd-ts-ce-25054_image_04-1024x186.jpg\" alt=\"\u30c4\u30fc\u30eb\u3092\u7528\u3044\u305fIndel\u89e3\u6790\u7d50\u679c\" class=\"wp-image-22903\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/08\/sanger-seq-ce-gsd-ts-ce-25054_image_04-1024x186.jpg 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/08\/sanger-seq-ce-gsd-ts-ce-25054_image_04-300x55.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/08\/sanger-seq-ce-gsd-ts-ce-25054_image_04-768x140.jpg 768w, 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class=\"wp-block-heading\" id=\"h-\u307e\u3068\u3081\">\u307e\u3068\u3081<\/h2>\n\n\n\n<p>\u30c0\u30a4\u30ec\u30af\u30c8\u30b7\u30fc\u30b1\u30f3\u30b9\u6cd5\u306f\u3001\u7c21\u4fbf\u306b\u5869\u57fa\u914d\u5217\u3092\u89e3\u6790\u3067\u304d\u308b\u624b\u6cd5\u3068\u3057\u3066\u6c4e\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u30d8\u30c6\u30ed\u63a5\u5408\u6027Indel\u30b5\u30f3\u30d7\u30eb\u306e\u89e3\u6790\u304c\u56f0\u96e3\u3067\u3042\u308b\u8ab2\u984c\u304c\u3042\u308a\u307e\u3057\u305f\u3002\u305d\u3053\u3067\u4eca\u56de\u306f\u3001\u3053\u306e\u8ab2\u984c\u3092\u514b\u670d\u3059\u308b\u30a2\u30d7\u30ed\u30fc\u30c1\u3068\u3057\u3066\u3001\u65b0\u3057\u3044\u30d9\u30fc\u30b9\u30b3\u30fc\u30e9\u30fc\u3067\u3042\u308bSDB\u3068\u3001\u5c02\u7528\u306e\u30c4\u30fc\u30eb\u3092\u7528\u3044\u305f\u89e3\u6790\u65b9\u6cd5\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3057\u305f\u3002Indel\u3092\u306f\u3058\u3081\u3068\u3059\u308b\u5909\u7570\u89e3\u6790\u306b\u304a\u56f0\u308a\u306e\u65b9\u3001\u672c\u89e3\u6790\u65b9\u6cd5\u306b\u3054\u8208\u5473\u3092\u304a\u6301\u3061\u306e\u65b9\u306f\u3001\u305c\u3072\u30c6\u30af\u30cb\u30ab\u30eb\u30b5\u30dd\u30fc\u30c8<a 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href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/technical-resources\/seminars\/mutation-analysis-sequencing.html?CID=gsd_pcr_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_sanger_seq_ce_gsd_ts_ce_25054_Social_LAB\" target=\"_blank\" rel=\"noreferrer noopener\">\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u3092\u7528\u3044\u305f\u5909\u7570\u89e3\u6790 \uff5e\u30bd\u30d5\u30c8\u30a6\u30a8\u30a2\u3092\u5229\u7528\u3057\u305f\u52b9\u7387\u5316\uff5e<\/a><br><a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/technical-resources\/trainings\/capillary-sequencer-3.html?CID=gsd_pcr_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_sanger_seq_ce_gsd_ts_ce_25054_Social_LAB\" target=\"_blank\" rel=\"noreferrer noopener\">\u5b8c\u5168\u30de\u30b9\u30bf\u30fc\uff01\u30cf\u30f3\u30ba\u30aa\u30f3\u30c8\u30ec\u30fc\u30cb\u30f3\u30b0\u30b3\u30fc\u30b9\u3000\u30ad\u30e3\u30d4\u30e9\u30ea\u30fc\u30b7\u30fc\u30b1\u30f3\u30b5\uff083\uff09\u30c7\u30fc\u30bf\u89e3\u6790 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