{"id":23665,"date":"2025-10-28T10:01:37","date_gmt":"2025-10-28T01:01:37","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/?p=23665"},"modified":"2025-10-28T10:01:40","modified_gmt":"2025-10-28T01:01:40","slug":"pcr-dna-gsd-ts-tr-25064","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/pcr-dna-gsd-ts-tr-25064\/","title":{"rendered":"\u3010\u3084\u3063\u3066\u307f\u305f\u3011PCR\u306e\u30a2\u30cb\u30fc\u30ea\u30f3\u30b0\u6e29\u5ea6\u3092\u601d\u3044\u5207\u308a\u632f\u3063\u3066\u307f\u305f"},"content":{"rendered":"\n<p>\u65b0\u305f\u306aPCR\u691c\u51fa\u7cfb\u3092\u69cb\u7bc9\u3059\u308b\u5834\u5408\u3001\u4f55\u3088\u308a\u3082\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u914d\u5217\u304c\u91cd\u8981\u3068\u3044\u3046\u3053\u3068\u306f\u591a\u304f\u306e\u65b9\u304c\u8a8d\u8b58\u3057\u3066\u3044\u308b\u306e\u3067\u306f\u306a\u3044\u3067\u3057\u3087\u3046\u304b\u3002\u9ad8\u6027\u80fd\u306a\u30d7\u30e9\u30a4\u30de\u30fc\u304c\u3067\u304d\u308c\u3070\u3001PCR\u6761\u4ef6\u304c\u3042\u307e\u308a\u6700\u9069\u5316\u3055\u308c\u3066\u3044\u306a\u304f\u3066\u3082\u9ad8\u54c1\u8cea\u306a\u7d50\u679c\u3092\u5f97\u308b\u3053\u3068\u306f\u96e3\u3057\u304f\u3042\u308a\u307e\u305b\u3093\u3002\u3082\u3057PCR\u304c\u3046\u307e\u304f\u3044\u304b\u306a\u3044\u5834\u5408\u306f\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u914d\u5217\u3092\u5909\u66f4\u3059\u308b\u3053\u3068\u3067\u5287\u7684\u306b\u3046\u307e\u304f\u3044\u304f\u3053\u3068\u3082\u3042\u308a\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u3057\u304b\u3057\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u914d\u5217\u3092\u5909\u3048\u308b\u3053\u3068\u304c\u3067\u304d\u306a\u3044\u5834\u5408\u306f\u3069\u3046\u3067\u3057\u3087\u3046\u304b\u3002\u305d\u306e\u5834\u5408\u3001PCR\u53cd\u5fdc\u306b\u95a2\u308f\u308b\u3055\u307e\u3056\u307e\u306a\u8981\u7d20\u3092\u691c\u8a0e\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u4f8b\u3048\u3070\u3001\u4f7f\u7528\u3059\u308bDNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u3001\u92f3\u578bDNA\u306e\u6295\u5165\u91cf\u3001\u4f38\u9577\u53cd\u5fdc\u306e\u6642\u9593\u306a\u3069\u306a\u3069\u3002\u7d30\u304b\u304f\u6319\u3052\u308c\u3070\u304d\u308a\u304c\u306a\u3044\u3067\u3059\u304c\u3001\u4e2d\u3067\u3082\u5f71\u97ff\u304c\u5927\u304d\u3044\u306e\u306f\u30a2\u30cb\u30fc\u30ea\u30f3\u30b0\u6e29\u5ea6\u3067\u306f\u306a\u3044\u3067\u3057\u3087\u3046\u304b\u3002\u30a2\u30cb\u30fc\u30ea\u30f3\u30b0\u6e29\u5ea6\u306f\u30d7\u30e9\u30a4\u30de\u30fc\u914d\u5217\u304b\u3089Nearest Neighbor\u6cd5\u306a\u3069\u306b\u3088\u308a\u7b97\u51fa\u3055\u308c\u308bTm\u5024\u3092\u76ee\u5b89\u306b\u8a2d\u5b9a\u3059\u308b\u3053\u3068\u304c\u4e00\u822c\u7684\u3067\u3059\u3002\u3057\u304b\u3057\u3053\u306eTm\u5024\u306f\u3042\u304f\u307e\u3067\u3082\u8a08\u7b97\u4e0a\u5f97\u3089\u308c\u308b\u5024\u306a\u306e\u3067\u3001\u5b9f\u969b\u306ePCR\u53cd\u5fdc\u3067\u306f\u30a2\u30cb\u30fc\u30ea\u30f3\u30b0\u6e29\u5ea6\u306e\u6700\u9069\u5316\u304c\u5fc5\u8981\u306b\u306a\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u672c\u8a18\u4e8b\u3067\u306f\u3001\u30a2\u30cb\u30fc\u30ea\u30f3\u30b0\u6e29\u5ea6\u3092\u5927\u304d\u304f\u632f\u3063\u3066\u307f\u3066PCR\u5897\u5e45\u7523\u7269\u304c\u3069\u306e\u3088\u3046\u306b\u5909\u5316\u3059\u308b\u304b\u3001\u691c\u8a3c\u3057\u3066\u307f\u307e\u3057\u305f\u3002\u3053\u306e\u7d50\u679c\u3092\u898b\u308c\u3070\u3001PCR\u53cd\u5fdc\u304c\u3046\u307e\u304f\u3044\u304b\u306a\u3044\u3068\u304d\u306b\u30a2\u30cb\u30fc\u30ea\u30f3\u30b0\u6e29\u5ea6\u3092\u3069\u3046\u6700\u9069\u5316\u3059\u308c\u3070\u3088\u3044\u304b\u304c\u5206\u304b\u308b\u3088\u3046\u306b\u306a\u308a\u307e\u3059\u3002<\/p>\n\n\n\n<div class=\"wp-block-group is-vertical is-layout-flex wp-container-core-group-is-layout-8cf370e7 wp-block-group-is-layout-flex\">\n<p><strong>\u25bc\u3053\u3093\u306a\u65b9\u306b\u304a\u3059\u3059\u3081\u3067\u3059\uff01<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>PCR\u6761\u4ef6\u306e\u6700\u9069\u5316\u306b\u3064\u3044\u3066\u77e5\u308a\u305f\u3044\u65b9<\/strong><\/li>\n<\/ul>\n<\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u6750\u6599\u3068\u65b9\u6cd5\">\u6750\u6599\u3068\u65b9\u6cd5<\/h2>\n\n\n\n<p>\u4ee5\u4e0b\u306e\u30ec\u30b7\u30d4\u306b\u5f93\u3044\u3001\u30a2\u30cb\u30fc\u30ea\u30f3\u30b0\u6e29\u5ea6\u309224\uff5e72 \u2103\u307e\u30673 \u2103\u523b\u307f\u3067\u5909\u5316\u3055\u305b\u3066PCR\u53cd\u5fdc\u5f8c\u3001\u30a2\u30ac\u30ed\u30fc\u30b9\u30b2\u30eb\u96fb\u6c17\u6cf3\u52d5\u3067\u30d0\u30f3\u30c9\u30d1\u30bf\u30fc\u30f3\u3092\u78ba\u8a8d\u3057\u307e\u3057\u305f\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-1-pcr\u53cd\u5fdc\u6db2\u306e\u7d44\u6210\">1\uff0ePCR\u53cd\u5fdc\u6db2\u306e\u7d44\u6210<\/h3>\n\n\n\n<div class=\"wp-block-group is-vertical is-layout-flex wp-container-core-group-is-layout-8cf370e7 wp-block-group-is-layout-flex\">\n<figure class=\"wp-block-table\">\n  <table class=\"has-fixed-layout\">\n    <thead>\n      <tr>\n        <th class=\"has-text-align-left\" data-align=\"left\"><strong>Component<\/strong><\/th>\n        <th class=\"has-text-align-left\" data-align=\"left\" style=\"width: 150px\"><strong>1\u53cd\u5fdc\u3042\u305f\u308a\uff08\u00b5L\uff09<\/strong><\/th>\n      <\/tr>\n    <\/thead>\n    <tbody>\n      <tr>\n        <td class=\"has-text-align-left\" data-align=\"left\">Applied Biosystems\u2122 AmpliTaq Gold\u2122 360 Master Mix \uff08\u88fd\u54c1\u756a\u53f7 4398876\uff09<\/td>\n        <td class=\"has-text-align-left\" data-align=\"left\" style=\"width: 150px\">25<\/td>\n      <\/tr>\n      <tr>\n        <td class=\"has-text-align-left\" data-align=\"left\">Forward &amp; Reverse Primer Mix 10 \u00b5M(\u7d42\u6fc3\u5ea6200 nM) <sup>\u203b1<\/sup><\/td>\n        <td class=\"has-text-align-left\" data-align=\"left\" style=\"width: 150px\">1<\/td>\n      <\/tr>\n      <tr>\n        <td class=\"has-text-align-left\" data-align=\"left\">Human genomic DNA(1 ng\/\u00b5L)<\/td>\n        <td class=\"has-text-align-left\" data-align=\"left\" style=\"width: 150px\">1<\/td>\n      <\/tr>\n      <tr>\n        <td class=\"has-text-align-left\" data-align=\"left\">Nuclease Free Water<\/td>\n        <td class=\"has-text-align-left\" data-align=\"left\" style=\"width: 150px\">23<\/td>\n      <\/tr>\n    <\/tbody>\n    <tfoot>\n      <tr>\n        <td class=\"has-text-align-left\" data-align=\"left\"><strong>Total<\/strong><\/td>\n        <td class=\"has-text-align-left\" data-align=\"left\" style=\"width: 150px\"><strong>50<\/strong><\/td>\n      <\/tr>\n    <\/tfoot>\n  <\/table>\n<\/figure>\n\n\n\n<p class=\"has-small-font-size\">\u203b1 \u30d7\u30e9\u30a4\u30de\u30fc\u306fCD44\u3001 EGFR\u3001 HPRT1\u3001 AAVS1\u306e4\u7a2e\u985e\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u30bb\u30c3\u30c8\u3092\u4f7f\u7528\u3002<\/p>\n<\/div>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-2-pcr\u6761\u4ef6\">2\uff0ePCR\u6761\u4ef6<\/h3>\n\n\n\n<figure class=\"wp-block-image aligncenter size-large is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"651\" src=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/10\/c6e2a542af7cd516e822b048e6650f1f-1024x651.jpg\" alt=\"\u30a2\u30cb\u30fc\u30ea\u30f3\u30b0\u6e29\u5ea6\u306e\u5909\u5316\u3092\u793a\u3059\u30b0\u30e9\u30d5\" class=\"wp-image-23670\" style=\"width:648px;height:auto\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/10\/c6e2a542af7cd516e822b048e6650f1f-1024x651.jpg 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/10\/c6e2a542af7cd516e822b048e6650f1f-300x191.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/10\/c6e2a542af7cd516e822b048e6650f1f-768x488.jpg 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/10\/c6e2a542af7cd516e822b048e6650f1f.jpg 1053w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>\u30a2\u30cb\u30fc\u30ea\u30f3\u30b0\u6e29\u5ea6\u306e\u5909\u5316\uff0824\uff5e72 \u2103\u30023 \u2103\u523b\u307f\u3067\u8a2d\u5b9a\uff09\u306f Applied Biosystems\u2122 VeritiPro\u2122 \u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306eVeriFlex\u2122\u6a5f\u80fd\u306b\u3088\u308a\u884c\u3044\u307e\u3057\u305f\u3002\u5168\u3066\u306e\u30a2\u30cb\u30fc\u30ea\u30f3\u30b0\u6e29\u5ea6\u306e\u6761\u4ef6\u3092\u8a66\u9a13\u3059\u308b\u305f\u3081\u3001PCR\u53cd\u5fdc\u306f3\u56de\u306b\u5206\u3051\u3066\u5b9f\u65bd\u3057\u307e\u3057\u305f\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-3-\u4f7f\u7528\u3057\u305f\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u914d\u5217\u3068tm\u5024\">3\uff0e\u4f7f\u7528\u3057\u305f\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u914d\u5217\u3068Tm\u5024<\/h3>\n\n\n\n<div class=\"wp-block-group is-vertical is-layout-flex wp-container-core-group-is-layout-8cf370e7 wp-block-group-is-layout-flex\">\n<div class=\"wp-block-group is-layout-constrained wp-block-group-is-layout-constrained\">\n<div class=\"wp-block-group is-layout-constrained wp-block-group-is-layout-constrained\">\n<div class=\"wp-block-group is-vertical is-layout-flex wp-container-core-group-is-layout-8cf370e7 wp-block-group-is-layout-flex\">\n<figure style=\"font-size:13px;width: 100%\" class=\"wp-block-table\">\n  <table class=\"has-fixed-layout\" style=\"width: 100%\">\n    <colgroup>\n      <col style=\"width: 60px\">\n      <col style=\"width: 200px\">\n      <col style=\"width: 80px\"> <!-- \u5de6\u304b\u30893\u5217\u76ee\u306e\u5e45\u3092\u72ed\u304f\u8a2d\u5b9a -->\n      <col style=\"width: 200px\">\n      <col style=\"width: 80px\"> <!-- \u53f3\u304b\u30892\u5217\u76ee\u306e\u5e45\u3092\u72ed\u304f\u8a2d\u5b9a -->\n      <col style=\"width: 50px\"> <!-- \u4e00\u756a\u53f3\u306e\u5217\u306e\u5e45\u3092\u72ed\u304f\u8a2d\u5b9a -->\n    <\/colgroup>\n    <thead>\n      <tr>\n        <th class=\"has-text-align-left\" data-align=\"left\"><strong>Gene Name<\/strong><\/th>\n        <th class=\"has-text-align-left\" data-align=\"left\"><strong>Forward Sequence<\/strong><\/th>\n        <th class=\"has-text-align-center\" data-align=\"center\"><strong>Forward<br>Tm\uff08\u2103\uff09<sup>\u203b2<\/sup><\/strong><\/th>\n        <th class=\"has-text-align-left\" data-align=\"left\"><strong>Reverse Sequence<\/strong><\/th>\n        <th class=\"has-text-align-center\" data-align=\"center\"><strong>Reverse<br>Tm\uff08\u2103\uff09<sup>\u203b2<\/sup><\/strong><\/th>\n        <th class=\"has-text-align-center\" data-align=\"center\"><strong>\u0394Tm\uff08\u2103\uff09<\/strong><\/th>\n      <\/tr>\n    <\/thead>\n    <tbody>\n      <tr>\n        <td class=\"has-text-align-left\" data-align=\"left\"><em>EGFR<\/em><\/td>\n        <td class=\"has-text-align-left\" data-align=\"left\">ATGGGTGAGTCTCTGTGTGG<\/td>\n        <td class=\"has-text-align-center\" data-align=\"center\">58.6<\/td>\n        <td class=\"has-text-align-left\" data-align=\"left\">CCCAGGCCTTTCTCCACTTA<\/td>\n        <td class=\"has-text-align-center\" data-align=\"center\">59.2<\/td>\n        <td class=\"has-text-align-center\" data-align=\"center\">0.6<\/td>\n      <\/tr>\n      <tr>\n        <td class=\"has-text-align-left\" data-align=\"left\"><em>CD44<\/em><\/td>\n        <td class=\"has-text-align-left\" data-align=\"left\">GTGCTGGGATTGTAGGCATG<\/td>\n        <td class=\"has-text-align-center\" data-align=\"center\">57.7<\/td>\n        <td class=\"has-text-align-left\" data-align=\"left\">ACGTGGGGTTGACTCCTTAG<\/td>\n        <td class=\"has-text-align-center\" data-align=\"center\">58.9<\/td>\n        <td class=\"has-text-align-center\" data-align=\"center\">1.2<\/td>\n      <\/tr>\n      <tr>\n        <td class=\"has-text-align-left\" data-align=\"left\"><em>HPRT1<\/em><\/td>\n        <td class=\"has-text-align-left\" data-align=\"left\">GGCGTCGTGATTAGTGATGAT<\/td>\n        <td class=\"has-text-align-center\" data-align=\"center\">59.1<\/td>\n        <td class=\"has-text-align-left\" data-align=\"left\">GCCCAAAGGGAACTGATAGTC<\/td>\n        <td class=\"has-text-align-center\" data-align=\"center\">60.5<\/td>\n        <td class=\"has-text-align-center\" data-align=\"center\">1.4<\/td>\n      <\/tr>\n      <tr>\n        <td class=\"has-text-align-left\" data-align=\"left\"><em>AAVS1<\/em><\/td>\n        <td class=\"has-text-align-left\" data-align=\"left\">GAATATGTCCCAGATAGCAC<\/td>\n        <td class=\"has-text-align-center\" data-align=\"center\">51.6<\/td>\n        <td class=\"has-text-align-left\" data-align=\"left\">GTTCTCAGTGGCCACCCTGC<\/td>\n        <td class=\"has-text-align-center\" data-align=\"center\">63.2<\/td>\n        <td class=\"has-text-align-center\" data-align=\"center\">11.6<\/td>\n      <\/tr>\n    <\/tbody>\n  <\/table>\n<\/figure>\n\n\n\n<p style=\"font-size:15px\">\u203b2 Tm\u5024\u306f<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/brands\/thermo-scientific\/molecular-biology\/molecular-biology-learning-center\/molecular-biology-resource-library\/thermo-scientific-web-tools\/tm-calculator.html?CID=gsd_pcr_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_pcr_dna_gsd_ts_tr_25064_Social_LAB\">Tm Calculat<\/a><a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/brands\/thermo-scientific\/molecular-biology\/molecular-biology-learning-center\/molecular-biology-resource-library\/thermo-scientific-web-tools\/tm-calculator.html?CID=gsd_pcr_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_pcr_dna_gsd_ts_tr_25064_Social_LAB\" target=\"_blank\" rel=\"noreferrer noopener\">o<\/a><a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/brands\/thermo-scientific\/molecular-biology\/molecular-biology-learning-center\/molecular-biology-resource-library\/thermo-scientific-web-tools\/tm-calculator.html?CID=gsd_pcr_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_pcr_dna_gsd_ts_tr_25064_Social_LAB\">r<\/a>\u3067\u7b97\u51fa\u3002<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u7d50\u679c\">\u7d50\u679c<\/h2>\n\n\n\n<p>\u305d\u308c\u3067\u306f\u6cf3\u52d5\u7d50\u679c\u3092\u898b\u306a\u304c\u3089\u8003\u5bdf\u3057\u3066\u3044\u304d\u307e\u3057\u3087\u3046\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image aligncenter size-large is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"396\" src=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/10\/527100c471feac20bee653299b088602-1024x396.jpg\" alt=\"HPRT1\u7d50\u679c\" class=\"wp-image-23676\" style=\"width:720px\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/10\/527100c471feac20bee653299b088602-1024x396.jpg 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/10\/527100c471feac20bee653299b088602-300x116.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/10\/527100c471feac20bee653299b088602-768x297.jpg 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/10\/527100c471feac20bee653299b088602.jpg 1252w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<figure class=\"wp-block-image aligncenter size-large is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"394\" src=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/10\/037b86758e93889ebf4779f648e8b434-1024x394.jpg\" alt=\"CD44\u7d50\u679c\" class=\"wp-image-23677\" style=\"width:720px\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/10\/037b86758e93889ebf4779f648e8b434-1024x394.jpg 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/10\/037b86758e93889ebf4779f648e8b434-300x115.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/10\/037b86758e93889ebf4779f648e8b434-768x295.jpg 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/10\/037b86758e93889ebf4779f648e8b434.jpg 1249w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<figure class=\"wp-block-image aligncenter size-large is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"386\" src=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/10\/d3e01b45f2e0e5c6692db34c50822899-1024x386.jpg\" alt=\"EGFR\u7d50\u679c\" class=\"wp-image-23679\" style=\"width:720px\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/10\/d3e01b45f2e0e5c6692db34c50822899-1024x386.jpg 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/10\/d3e01b45f2e0e5c6692db34c50822899-300x113.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/10\/d3e01b45f2e0e5c6692db34c50822899-768x290.jpg 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/10\/d3e01b45f2e0e5c6692db34c50822899.jpg 1252w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<div class=\"wp-block-group is-vertical is-content-justification-center is-layout-flex wp-container-core-group-is-layout-4b2eccd6 wp-block-group-is-layout-flex\">\n<div class=\"wp-block-group is-vertical is-content-justification-center is-layout-flex wp-container-core-group-is-layout-4b2eccd6 wp-block-group-is-layout-flex\">\n<figure class=\"wp-block-image aligncenter size-large is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"387\" src=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/10\/96b7d559c843467f97f93e592d9da339-1024x387.jpg\" alt=\"AAVS1\u7d50\u679c\" class=\"wp-image-23681\" style=\"width:720px\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/10\/96b7d559c843467f97f93e592d9da339-1024x387.jpg 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/10\/96b7d559c843467f97f93e592d9da339-300x113.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/10\/96b7d559c843467f97f93e592d9da339-768x290.jpg 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/10\/96b7d559c843467f97f93e592d9da339.jpg 1249w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<p class=\"has-text-align-center\"><strong>\u56f3. \u5404PCR\u6761\u4ef6\u306e\u30a2\u30ac\u30ed\u30fc\u30b9\u30b2\u30eb\u96fb\u6c17\u6cf3\u52d5\u50cf<\/strong><\/p>\n<\/div>\n<\/div>\n\n\n\n<p style=\"font-size:15px\">\u8d64\u77e2\u982d\uff1a\u7279\u7570\u7684\u306a\u30d0\u30f3\u30c9<br>*\uff1a\u8a08\u7b97\u4e0a\u306eTm\u5024\u306e\u8fd1\u4f3c\u5024\uff08<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/brands\/thermo-scientific\/molecular-biology\/molecular-biology-learning-center\/molecular-biology-resource-library\/thermo-scientific-web-tools\/tm-calculator.html?CID=gsd_pcr_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_pcr_dna_gsd_ts_tr_25064_Social_LAB\" target=\"_blank\" rel=\"noreferrer noopener\">Tm Calculator<\/a>\u3067\u7b97\u51fa\u3057\u305fForward\u3068Reverse\u306e\u30d7\u30e9\u30a4\u30de\u30fcTm\u5024\u306e\u5e73\u5747\uff09<br>\u6cf3\u52d5\u50cf\u4e0a\u90e8\u306e\u6570\u5024\uff1a\u30a2\u30cb\u30fc\u30ea\u30f3\u30b0\u6e29\u5ea6\uff08\u2103\uff09<br>\u6cf3\u52d5\u50cf\u4e0a\u90e8\u306e\u9752\u7dda\uff1a\u660e\u77ad\u306a\u30b7\u30f3\u30b0\u30eb\u30d0\u30f3\u30c9\u3068\u3057\u3066\u691c\u51fa\u3055\u308c\u305f\u30ec\u30fc\u30f3<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-1-\u30a2\u30cb\u30fc\u30ea\u30f3\u30b0\u6e29\u5ea6\u3068\u7279\u7570\u7684\u30d0\u30f3\u30c9\u306e\u95a2\u4fc2\">1\uff0e\u30a2\u30cb\u30fc\u30ea\u30f3\u30b0\u6e29\u5ea6\u3068\u7279\u7570\u7684\u30d0\u30f3\u30c9\u306e\u95a2\u4fc2<\/h3>\n\n\n\n<p>\u307e\u305a\u3001\u5404\u6cf3\u52d5\u50cf\u306b\u304a\u3044\u3066\u60f3\u5b9a\u3055\u308c\u308b\u7279\u7570\u7684\u306a\u30d0\u30f3\u30c9\u306f\u8d64\u77e2\u982d\u3067\u793a\u3057\u3066\u3044\u3057\u307e\u3059\u3002\u30a2\u30cb\u30fc\u30ea\u30f3\u30b0\u6e29\u5ea6\u304c\u9ad8\u3044\u5834\u5408\u306f\u7279\u7570\u7684\u306a\u30d0\u30f3\u30c9\u3082\u542b\u3081\u3001\u4f55\u3082\u691c\u51fa\u3055\u308c\u3066\u3044\u307e\u305b\u3093\u3002PCR\u3067\u5897\u5e45\u3059\u308b\u305f\u3081\u306b\u306f\u30d7\u30e9\u30a4\u30de\u30fc\u304c\u92f3\u578bDNA\u306b\u7d50\u5408\uff08\u30a2\u30cb\u30fc\u30eb\uff09\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u304c\u3001\u6e29\u5ea6\u304c\u9ad8\u3059\u304e\u308b\u3068\u30d7\u30e9\u30a4\u30de\u30fc\u304c\u7d50\u5408\u3067\u304d\u305a\u3001\u7d50\u679c\u7684\u306b\u5897\u5e45\u3057\u306a\u3044\u305f\u3081\u3067\u3059\u3002<\/p>\n\n\n\n<p>\u4e00\u65b9\u3001\u30a2\u30cb\u30fc\u30ea\u30f3\u30b0\u6e29\u5ea6\u304c\u4f4e\u3044\u5834\u5408\u306f\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u30bb\u30c3\u30c84\u7a2e\u306e\u5168\u3066\u3067\u975e\u7279\u7570\u7684\u306a\u30d0\u30f3\u30c9\u304c\u9855\u8457\u306b\u5897\u52a0\u3057\u307e\u3057\u305f\u3002\u3053\u308c\u306f\u3001\u6e29\u5ea6\u304c\u4f4e\u3044\u3053\u3068\u3067\u30d7\u30e9\u30a4\u30de\u30fc\u304c\u7279\u7570\u7684\u306a\u914d\u5217\u4ee5\u5916\u306b\u3082\u7d50\u5408\u3057\u3001\u672c\u6765\u5897\u3048\u3066\u307b\u3057\u304f\u306a\u3044\u5897\u5e45\u304c\u8d77\u3053\u3063\u305f\u305f\u3081\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u6cf3\u52d5\u7d50\u679c\u3092\u8a73\u3057\u304f\u898b\u308b\u3068\u3001\u3069\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u30bb\u30c3\u30c8\u3067\u3082\u4f4e\u6e29\u5ea6\u5e2f\uff08\u975e\u7279\u7570\u7684\u306b\u5897\u5e45\u3059\u308b\uff09\u3068\u9ad8\u6e29\u5ea6\u5e2f\uff08\u30d0\u30f3\u30c9\u304c\u691c\u51fa\u3055\u308c\u306a\u3044\uff09\u306e\u9593\u306e\u9069\u5207\u306a\u6e29\u5ea6\u5e2f\u306b\u304a\u3044\u3066\u3001\u672c\u6765\u5897\u3048\u308b\u3079\u304d\u30d0\u30f3\u30c9\u306e\u307f\u304c\u691c\u51fa\u3055\u308c\u3066\u3044\u308b\u3053\u3068\u304c\u5206\u304b\u308a\u307e\u3059\u3002<\/p>\n\n\n\n<div class=\"wp-block-group is-vertical is-layout-flex wp-container-core-group-is-layout-8cf370e7 wp-block-group-is-layout-flex\">\n<p>\u3053\u306e\u3053\u3068\u304b\u3089\u3001PCR\u6761\u4ef6\u3092\u6700\u9069\u5316\u3059\u308b\u305f\u3081\u306b\u306f\u4ee5\u4e0b\u306e\u3088\u3046\u306a\u8003\u3048\u65b9\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li>\u975e\u7279\u7570\u7684\u306a\u30d0\u30f3\u30c9\u304c\u3042\u308b\u5834\u5408\u2192\u30a2\u30cb\u30fc\u30ea\u30f3\u30b0\u6e29\u5ea6\u3092\u4e0a\u3052\u308b<\/li>\n\n\n\n<li>\u76ee\u7684\u306e\u30d0\u30f3\u30c9\u304c\u691c\u51fa\u3055\u308c\u306a\u3044\u5834\u5408\u2192\u30a2\u30cb\u30fc\u30ea\u30f3\u30b0\u6e29\u5ea6\u3092\u4e0b\u3052\u308b<\/li>\n<\/ol>\n<\/div>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-2-\u6975\u7aef\u306b\u4f4e\u3044\u30a2\u30cb\u30fc\u30ea\u30f3\u30b0\u6e29\u5ea6\u306e\u5f71\u97ff\">2\uff0e\u6975\u7aef\u306b\u4f4e\u3044\u30a2\u30cb\u30fc\u30ea\u30f3\u30b0\u6e29\u5ea6\u306e\u5f71\u97ff<\/h3>\n\n\n\n<p>\u8208\u5473\u6df1\u3044\u3053\u3068\u306b\u3001\u30a2\u30cb\u30fc\u30ea\u30f3\u30b0\u6e29\u5ea6\u304c\u6975\u7aef\u306b\u4f4e\u3044\uff0824 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class=\"wp-block-heading\" 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