{"id":24647,"date":"2026-02-09T18:08:40","date_gmt":"2026-02-09T09:08:40","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/?p=24647"},"modified":"2026-02-09T18:08:43","modified_gmt":"2026-02-09T09:08:43","slug":"sanger-seq-mutational-analysis-gsd-ts-ce-26007","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/sanger-seq-mutational-analysis-gsd-ts-ce-26007\/","title":{"rendered":"\u30de\u30a4\u30ca\u30fc\u5909\u7570\u3092\u691c\u51fa\uff01Minor Variant Finder\u30bd\u30d5\u30c8\u30a6\u30a8\u30a2"},"content":{"rendered":"\n

\u30ad\u30e3\u30d4\u30e9\u30ea\u30fc\u30b7\u30fc\u30b1\u30f3\u30b5\u3067\u306f\u300125%\u7a0b\u5ea6\u306e\u5272\u5408\u306e\u5909\u7570\u3092\u691c\u51fa\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u304c\u3001\u3053\u308c\u3088\u308a\u3082\u4f4e\u3044\u5272\u5408\u306e\u5909\u7570\u691c\u51fa\u306f\u6975\u3081\u3066\u56f0\u96e3\u3067\u3059\u3002\u3057\u304b\u3057\u306a\u304c\u3089\u3001\u816b\u760d\u5b66\u3084\u611f\u67d3\u75c7\u5b66\u306a\u3069\u306e\u7814\u7a76\u5206\u91ce\u306b\u304a\u3044\u3066\u3001\u30de\u30a4\u30ca\u30fc\u5909\u7570\u3092\u691c\u51fa\u3059\u308b\u3053\u3068\u306f\u975e\u5e38\u306b\u91cd\u8981\u3067\u3059\u3002
\u305d\u3053\u3067\u4eca\u56de\u306f\u3001\u30ad\u30e3\u30d4\u30e9\u30ea\u30fc\u30b7\u30fc\u30b1\u30f3\u30b5\u30c7\u30fc\u30bf\u3067\u30de\u30a4\u30ca\u30fc\u5909\u7570\u3092\u691c\u51fa\u3059\u308b\u305f\u3081\u306b\u958b\u767a\u3055\u308c\u305fApplied Biosystems\u2122 Minor Variant Finder\u30bd\u30d5\u30c8\u30a6\u30a8\u30a2<\/a>\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n\n\n\n

\u25bc\u3053\u3093\u306a\u65b9\u306b\u304a\u3059\u3059\u3081\u3067\u3059\uff01
\u30fb\u30ad\u30e3\u30d4\u30e9\u30ea\u30fc\u30b7\u30fc\u30b1\u30f3\u30b5\u3067\u30de\u30a4\u30ca\u30fc\u5909\u7570\u3092\u89e3\u6790\u3057\u305f\u3044\u65b9
\u30fb\u6b21\u4e16\u4ee3\u30b7\u30fc\u30b1\u30f3\u30b5\uff08NGS\uff09\u3067\u691c\u51fa\u3057\u305f\u30de\u30a4\u30ca\u30fc\u5909\u7570\u3092\u30ad\u30e3\u30d4\u30e9\u30ea\u30fc\u30b7\u30fc\u30b1\u30f3\u30b5\u3067\u78ba\u8a8d\u3057\u305f\u3044\u65b9<\/p>\n\n\n\n

\u30de\u30a4\u30ca\u30fc\u5909\u7570\u3092\u691c\u51fa\u3059\u308bMinor Variant Finder\u30bd\u30d5\u30c8\u30a6\u30a8\u30a2\u3068\u306f\uff1f<\/h2>\n\n\n\n

\u30ad\u30e3\u30d4\u30e9\u30ea\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u306e\u30c7\u30fc\u30bf\u306b\u306f\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9\u306b\u30ce\u30a4\u30ba\u304c\u751f\u3058\u307e\u3059\u3002\u5909\u7570\u306e\u5272\u5408\u304c\u4f4e\u3044\u5834\u5408\u306f\u5909\u7570\u30d4\u30fc\u30af\u304c\u5c0f\u3055\u304f\u3001\u30ce\u30a4\u30ba\u306b\u57cb\u3082\u308c\u3066\u3057\u307e\u3044\u3001\u30ce\u30a4\u30ba\u3068\u5909\u7570\u30d4\u30fc\u30af\u3092\u898b\u5206\u3051\u308b\u3053\u3068\u304c\u56f0\u96e3\u306b\u306a\u308a\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u3001\u5f93\u6765\u306e\u30bd\u30d5\u30c8\u30a6\u30a8\u30a2\u3067\u306f\u3001\u30ad\u30e3\u30d4\u30e9\u30ea\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u3067\u5909\u7570\u3092\u691c\u51fa\u3059\u308b\u306b\u306f\u300125\uff05\u7a0b\u5ea6\u4ee5\u4e0a\u306e\u5272\u5408\u3067\u5909\u7570\u30d4\u30fc\u30af\u304c\u5b58\u5728\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3057\u305f\u3002
Minor Variant Finder\u30bd\u30d5\u30c8\u30a6\u30a8\u30a2\u306f\u3001\u5f53\u793e\u306e\u9577\u5e74\u306b\u308f\u305f\u308b\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\u6280\u8853\u306e\u84c4\u7a4d\u3092\u57fa\u306b\u958b\u767a\u3057\u305f\u9769\u65b0\u7684\u306a\u30a2\u30eb\u30b4\u30ea\u30ba\u30e0\u3092\u642d\u8f09\u3057\u3066\u3044\u307e\u3059\u3002\u3053\u306e\u30a2\u30eb\u30b4\u30ea\u30ba\u30e0\u306b\u3088\u308a\u3001\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u30b5\u30f3\u30d7\u30eb\u304b\u3089\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9\u306e\u30ce\u30a4\u30ba\u304c\u8b58\u5225\u3055\u308c\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u30b5\u30f3\u30d7\u30eb\u306e\u30ce\u30a4\u30ba\u3092\u6700\u5c0f\u5316\u3059\u308b\u30d7\u30ed\u30bb\u30b9\u304c\u884c\u308f\u308c\u307e\u3059\u3002\u3053\u306e\u7d50\u679c\u3001\u8b58\u5225\u304c\u56f0\u96e3\u306a\u30de\u30a4\u30ca\u30fc\u5909\u7570\u306e\u30d4\u30fc\u30af\u3092\u8996\u899a\u7684\u306b\u6349\u3048\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u3088\u3046\u306b\u306a\u308a\u307e\u3059\u3002\u3055\u3089\u306b\u30d5\u30a9\u30ef\u30fc\u30c9\u304a\u3088\u3073\u30ea\u30d0\u30fc\u30b9\u914d\u5217\u306e\u4e21\u65b9\u306e\u30c7\u30fc\u30bf\u304b\u3089\u5909\u7570\u306e\u691c\u51fa\u3092\u884c\u3046\u3053\u3068\u3067\u3001\u30de\u30a4\u30ca\u30fc\u5909\u7570\uff085%\u7a0b\u5ea6\uff09\u306e\u691c\u51fa\u3092\u53ef\u80fd\u306b\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n\n\n\n

\n
\n
\n
\n
\"\u56f31.<\/figure>\n\n\n\n

\u56f31. \u30aa\u30ea\u30b8\u30ca\u30eb\u30c7\u30fc\u30bf\uff08\u4e0a\u6bb5\uff09\u3068Minor Variant Finder\u30bd\u30d5\u30c8\u30a6\u30a8\u30a2\u3067\u89e3\u6790\u3057\u305f\u30c7\u30fc\u30bf\uff08\u4e0b\u6bb5\uff09<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n

<\/div>\n\n\n\n

\u30b7\u30f3\u30d7\u30eb\u306a\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u3067\u5909\u7570\u3092\u691c\u51fa\uff01<\/h2>\n\n\n\n

Minor Variant Finder\u30bd\u30d5\u30c8\u30a6\u30a8\u30a2\u3067\u89e3\u6790\u3059\u308b\u305f\u3081\u306b\u3001\u30ad\u30e3\u30d4\u30e9\u30ea\u30fc\u30b7\u30fc\u30b1\u30f3\u30b5\u306e\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u3092\u5909\u66f4\u3059\u308b\u5fc5\u8981\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u7279\u5225\u306a\u8a66\u85ac\u3092\u4f7f\u7528\u3057\u305f\u308a\u3001\u30d7\u30ed\u30c8\u30b3\u30eb\u3092\u5909\u66f4\u3057\u305f\u308a\u3059\u308b\u3053\u3068\u306a\u304f\u3001\u5f53\u793e\u306e\u30b8\u30a7\u30cd\u30c6\u30a3\u30c3\u30af\u30a2\u30ca\u30e9\u30a4\u30b6\u3067\u53d6\u5f97\u3057\u305f\u30b7\u30fc\u30b1\u30f3\u30b9\u30c7\u30fc\u30bf\u30d5\u30a1\u30a4\u30eb\u3092\u3053\u306e\u30bd\u30d5\u30c8\u30a6\u30a8\u30a2\u3067\u89e3\u6790\u3044\u305f\u3060\u3051\u307e\u3059\u3002
\u6ce8\u610f\u4e8b\u9805\uff1a \u89e3\u6790\u3067\u304d\u306a\u3044\u6a5f\u7a2e\u304c\u3054\u3056\u3044\u307e\u3059\u306e\u3067\u3001\u89e3\u6790\u306e\u524d\u306b\u3054\u78ba\u8a8d\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n

\n
\n
\n
\"\u56f32.<\/figure>\n\n\n\n

\u56f32. \u89e3\u6790\u30d5\u30ed\u30fc<\/p>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n

<\/div>\n\n\n\n

Step 1\uff1a\u30b5\u30f3\u30d7\u30eb\u3092\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0<\/strong>
\u30bf\u30fc\u30b2\u30c3\u30c8\u30b5\u30f3\u30d7\u30eb\u3068\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u30b5\u30f3\u30d7\u30eb\u3092\u3001\u30d5\u30a9\u30ef\u30fc\u30c9\u30d7\u30e9\u30a4\u30de\u30fc\u3068\u30ea\u30d0\u30fc\u30b9\u30d7\u30e9\u30a4\u30de\u30fc\u3067\u305d\u308c\u305e\u308c\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\u3092\u884c\u3044\u307e\u3059\u3002<\/p>\n\n\n\n

\u6ce8\u610f\u4e8b\u9805\uff1a\u3053\u308c\u3089\u306e\u30b5\u30f3\u30d7\u30eb\u306f\u5168\u3066\u540c\u3058\u30a4\u30f3\u30b8\u30a7\u30af\u30b7\u30e7\u30f3\u306b\u3066\u30e9\u30f3\u3092\u884c\u3046\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u7570\u306a\u308b\u30a4\u30f3\u30b8\u30a7\u30af\u30b7\u30e7\u30f3\u306e\u5834\u5408\u3001\u30ce\u30a4\u30ba\u306e\u6700\u5c0f\u5316\u30d7\u30ed\u30bb\u30b9\u306b\u5f71\u97ff\u3057\u3001\u9069\u5207\u306b\u89e3\u6790\u3067\u304d\u307e\u305b\u3093\u3002<\/p>\n\n\n\n

Step 2\uff1aMinor Variant Finder\u30bd\u30d5\u30c8\u30a6\u30a8\u30a2\u3067\u7c21\u5358\u306b\u89e3\u6790<\/strong>
Minor Variant Finder\u30bd\u30d5\u30c8\u30a6\u30a8\u30a2\u3067\u30d7\u30ed\u30b8\u30a7\u30af\u30c8\u3092\u4f5c\u6210\u3057\u305f\u5f8c\u3001\u30b7\u30fc\u30b1\u30f3\u30b9\u30c7\u30fc\u30bf\u3092\u6307\u5b9a\u3057\u3066\u89e3\u6790\u3092\u5b9f\u884c\u3057\u307e\u3059\u3002
\u89e3\u6790\u5f8c\u306f\u30ce\u30a4\u30ba\u3092\u6700\u5c0f\u5316\u3057\u305f\u30bf\u30fc\u30b2\u30c3\u30c8\u30b5\u30f3\u30d7\u30eb\u306e\u6ce2\u5f62\u3084\u3001\u691c\u51fa\u3055\u308c\u305f\u5909\u7570\u3092\u78ba\u8a8d\u3067\u304d\u307e\u3059\u3002<\/p>\n\n\n\n

\n
\n
\n
\n
\n
\"\u56f33.<\/figure>\n\n\n\n

\u56f33. Minor Variant Finder\u30bd\u30d5\u30c8\u30a6\u30a8\u30a2\u3067\u306e\u89e3\u6790\u7d50\u679c<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n

<\/div>\n<\/div>\n\n\n\n
<\/div>\n\n\n\n
\n
\n
\n
\"\u56f34.<\/figure>\n\n\n\n

\u56f34. Minor Variant Finder\u30bd\u30d5\u30c8\u30a6\u30a8\u30a2\u3067\u306e\u89e3\u6790\u7d50\u679c\uff1a\u6ce2\u5f62\u30c7\u30fc\u30bf\u306e\u78ba\u8a8d<\/p>\n<\/div>\n\n\n\n

<\/p>\n<\/div>\n<\/div>\n\n\n\n

<\/div>\n\n\n\n

\u6b21\u4e16\u4ee3\u30b7\u30fc\u30b1\u30f3\u30b5(NGS)\u3068\u306e\u6bd4\u8f03\u3082\u7c21\u5358\u306b\uff01<\/h2>\n\n\n\n

Minor Variant Finder\u30bd\u30d5\u30c8\u30a6\u30a8\u30a2\u306f\u3001NGS\u306e\u30c7\u30fc\u30bf\uff08.vcf\u30d5\u30a1\u30a4\u30eb\uff09\u3092\u30a4\u30f3\u30dd\u30fc\u30c8\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u3001\u30ad\u30e3\u30d4\u30e9\u30ea\u30fc\u30b7\u30fc\u30b1\u30f3\u30b5\u3067\u5f97\u3089\u308c\u305f\u5909\u7570\u306b\u30a2\u30ce\u30c6\u30fc\u30b7\u30e7\u30f3\u4ed8\u3051\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u30a2\u30e9\u30a4\u30e1\u30f3\u30c8\u753b\u9762\u3068\u30d9\u30f3\u30c0\u30a4\u30a2\u30b0\u30e9\u30e0\u3067\u30c7\u30fc\u30bf\u3092\u53ef\u8996\u5316\u3057\u3001NGS\u30c7\u30fc\u30bf\u306e\u8fc5\u901f\u306a\u691c\u8a3c\u3092\u30b5\u30dd\u30fc\u30c8\u3057\u307e\u3059\u3002<\/p>\n\n\n\n

\n
\n
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\"\u56f35.<\/figure>\n\n\n\n

\u56f35. NGS\u3068\u306e\u6bd4\u8f03<\/p>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n

<\/div>\n\n\n\n

\u307e\u3068\u3081<\/h2>\n\n\n\n

\u4eca\u56de\u306f\u30b3\u30b9\u30c8\u52b9\u7387\u306e\u826f\u3044\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u3067\u30de\u30a4\u30ca\u30fc\u5909\u7570\u306e\u691c\u51fa\u3092\u53ef\u80fd\u306b\u3059\u308bMinor Variant Finder\u30bd\u30d5\u30c8\u30a6\u30a8\u30a2\u3092\u3054\u7d39\u4ecb\u3044\u305f\u3057\u307e\u3057\u305f\u3002<\/p>\n\n\n\n

\u30fb\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u30b5\u30f3\u30d7\u30eb\u306e\u30c7\u30fc\u30bf\u3092\u7528\u3044\u3066\u30bf\u30fc\u30b2\u30c3\u30c8\u30b5\u30f3\u30d7\u30eb\u306e\u30ce\u30a4\u30ba\u3092\u6700\u5c0f\u5316
\u30fb5%\u7a0b\u5ea6\u306e\u30de\u30a4\u30ca\u30fc\u5909\u7570\u3092\u691c\u51fa
\u30fb\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u306e\u30d7\u30ed\u30c8\u30b3\u30eb\u306e\u5909\u66f4\u306f\u4e0d\u8981
\u30fbNGS\u3068\u306e\u6bd4\u8f03\u3084\u30a2\u30ce\u30c6\u30fc\u30b7\u30e7\u30f3\u3082\u7c21\u5358\u306b\u884c\u3048\u308b<\/p>\n\n\n\n

\u30ad\u30e3\u30d4\u30e9\u30ea\u30fc\u30b7\u30fc\u30b1\u30f3\u30b5\u3067\u30de\u30a4\u30ca\u30fc\u5909\u7570\u306e\u78ba\u8a8d\u3092\u884c\u3044\u305f\u3044\u65b9\u306f\u3001\u7121\u6599\u306eMinor Variant Finder\u30bd\u30d5\u30c8\u30a6\u30a8\u30a2\u3092\u305c\u3072\u3054\u6d3b\u7528\u304f\u3060\u3055\u3044\u3002<\/h4>\n\n\n\n
<\/div>\n\n\n\n

\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u306f\u3053\u3061\u3089<\/a><\/p>\n\n\n\n

\n
<\/div>\n\n\n\n

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