{"id":258,"date":"2020-03-04T00:00:00","date_gmt":"2020-03-03T15:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic23\/"},"modified":"2025-07-01T17:42:46","modified_gmt":"2025-07-01T08:42:46","slug":"protein-basic23","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic23\/","title":{"rendered":"\u5171\u514d\u75ab\u6c88\u964d\u5b9f\u9a13\u306b\u304a\u3051\u308b\u30d0\u30c3\u30d5\u30a1\u30fc\u306b\u3064\u3044\u3066\uff5c\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\uff01\u30bf\u30f3\u30d1\u30af\u8cea\u5b9f\u9a13\u3042\u308c\u3053\u308c \u7b2c23\u56de"},"content":{"rendered":"<p>\u4eca\u56de\u3082\u3001<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic21\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa29_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u524d\u3005\u56de<\/a>\u3068<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic22\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa29_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u524d\u56de<\/a>\u306b\u5f15\u304d\u7d9a\u304d\u3001\uff08\u5171\uff09\u514d\u75ab\u6c88\u964d\u6cd5\u306b\u95a2\u3059\u308b\u57fa\u672c\u60c5\u5831\u3084\u30dd\u30a4\u30f3\u30c8\u306b\u3064\u3044\u3066\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n<h2>\uff08\u5171\uff09\u514d\u75ab\u6c88\u964d\u306b\u95a2\u4fc2\u3059\u308b\u30d5\u30a1\u30af\u30bf\u30fc<\/h2>\n<p>\u524d\u56de\u3082\u3054\u8aac\u660e\u3057\u307e\u3057\u305f\u304c\u3001\uff08\u5171\uff09\u514d\u75ab\u6c88\u964d\u6cd5\u306f\u6bd4\u8f03\u7684\u30b7\u30f3\u30d7\u30eb\u306a\u624b\u6cd5\u3067\u3059\u304c\u3001\u305d\u308c\u3067\u3082\u4e0b\u306e\u8868\u306b\u793a\u3059\u3088\u3046\u306a\u8907\u6570\u306e\u30d5\u30a1\u30af\u30bf\u30fc\u304c\u95a2\u4fc2\u3057\u3066\u3044\u307e\u3059\u3002\u5b9f\u9a13\u3092\u6210\u529f\u3055\u305b\u308b\u305f\u3081\u306b\u3082\u5404\u30d5\u30a1\u30af\u30bf\u30fc\u306b\u3064\u3044\u3066\u7406\u89e3\u3057\u3001\u30de\u30c6\u30ea\u30a2\u30eb\u306e\u9078\u629e\u3001\u6297\u4f53\u56fa\u5b9a\u5316\u3084\u30d0\u30c3\u30d5\u30a1\u30fc\u306e\u8abf\u88fd\u3092\u3001\u305d\u308c\u305e\u308c\u76ee\u7684\u306b\u5408\u308f\u305b\u3066\u884c\u3046\u3053\u3068\u304c\u671b\u307e\u308c\u307e\u3059\u3002\u4ee5\u4e0b\u3067\u306f\u3001\u305d\u308c\u305e\u308c\u306e\u30d5\u30a1\u30af\u30bf\u30fc\u306b\u3064\u3044\u3066\u500b\u5225\u306b\u3054\u7d39\u4ecb\u3057\u307e\u3059\uff08<strong>\u4eca\u56de\u306f\u300e\u7d50\u5408\u30d0\u30c3\u30d5\u30a1\u30fc\u300f\u3001\u300e\u6d17\u6d44\u30d0\u30c3\u30d5\u30a1\u30fc\u300f\u3001\u300e\u6eb6\u51fa\u30d0\u30c3\u30d5\u30a1\u30fc\u300f\u306e\u5404\u7a2e\u30d0\u30c3\u30d5\u30a1\u30fc\u306b\u3064\u3044\u3066\u8aac\u660e\u3057\u307e\u3059\u3002<\/strong>\u300e\u5b9f\u9a13\u30d5\u30a9\u30fc\u30de\u30c3\u30c8\u300f\u301c\u300e\u30e9\u30a4\u30bb\u30fc\u30c8\u306e\u524d\u51e6\u7406\u300f\u306b\u3064\u3044\u3066\u306f<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic21\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa29_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u524d\u3005\u56de<\/a>\u3001\u300e\u6297\u4f53\u306e\u56fa\u5b9a\u5316\u300f\u306b\u3064\u3044\u3066\u306f<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic22\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa29_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u524d\u56de<\/a>\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002\uff09\u3002<\/p>\n<table>\n<tbody>\n<tr>\n<td>\u30d5\u30a1\u30af\u30bf\u30fc<\/td>\n<td>\u95a2\u9023\u3059\u308b\u5185\u5bb9<\/td>\n<\/tr>\n<tr>\n<td>\u5b9f\u9a13\u30d5\u30a9\u30fc\u30de\u30c3\u30c8<\/td>\n<td>\u30ab\u30e9\u30e0\u6cd5\uff08\u30b9\u30d4\u30f3\u30ab\u30e9\u30e0\u3001\u30aa\u30fc\u30d7\u30f3\u30ab\u30e9\u30e0\uff09\u3001\u30d0\u30c3\u30c1\u6cd5<\/td>\n<\/tr>\n<tr>\n<td>\u62c5\u4f53\u306e\u7a2e\u985e<\/td>\n<td>\u30a2\u30ac\u30ed\u30fc\u30b9\u62c5\u4f53\u3001\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c9\u62c5\u4f53\u3001\u78c1\u6027\u62c5\u4f53<\/td>\n<\/tr>\n<tr>\n<td>\u53cd\u5fdc\u624b\u9806<\/td>\n<td>\u6297\u539f\u6297\u4f53\u53cd\u5fdc\u3001\u6297\u4f53\u306e\u62c5\u4f53\u3078\u306e\u7d50\u5408<\/td>\n<\/tr>\n<tr>\n<td>\u30e9\u30a4\u30bb\u30fc\u30c8\u306e\u524d\u51e6\u7406<\/td>\n<td>\u975e\u7279\u7570\u7d50\u5408<\/td>\n<\/tr>\n<tr>\n<td>\u6297\u4f53\u306e\u56fa\u5b9a\u5316<\/td>\n<td>\u56fa\u5b9a\u5316\u65b9\u6cd5\u3001\u5411\u304d\u3001\u91cf<\/td>\n<\/tr>\n<tr>\n<td>\u7d50\u5408\u30d0\u30c3\u30d5\u30a1\u30fc<\/td>\n<td>\u6297\u539f\u6297\u4f53\u53cd\u5fdc\u6761\u4ef6\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u8907\u5408\u4f53\u5f62\u6210\u6761\u4ef6<\/td>\n<\/tr>\n<tr>\n<td>\u6d17\u6d44\u30d0\u30c3\u30d5\u30a1\u30fc<\/td>\n<td>\u754c\u9762\u6d3b\u6027\u5264\u3001\u5869\u3001\u9084\u5143\u5264<\/td>\n<\/tr>\n<tr>\n<td>\u6eb6\u51fa\u30d0\u30c3\u30d5\u30a1\u30fc<\/td>\n<td>pH\u3001\u5869\u3001\u5909\u6027\u5264<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h3>\u7d50\u5408\u30d0\u30c3\u30d5\u30a1\u30fc<\/h3>\n<p>\u514d\u75ab\u6c88\u964d\u306b\u304a\u3051\u308b\u7d50\u5408\u30d0\u30c3\u30d5\u30a1\u30fc\u306e\u6761\u4ef6\u3068\u3057\u3066\u3001<strong>\u6297\u539f\u3068\u6297\u4f53\u3068\u306e\u30a2\u30d5\u30a3\u30cb\u30c6\u30a3\u30fc\u76f8\u4e92\u4f5c\u7528\u3092\u963b\u5bb3\u3057\u306a\u3044\u3053\u3068<\/strong>\u304c\u6c42\u3081\u3089\u308c\u307e\u3059\u3002\u3082\u3061\u308d\u3093\u3001\u5171\u514d\u75ab\u6c88\u964d\u306e\u5834\u5408\u306f\u3001IP\u6297\u4f53\u3068\u53cd\u5fdc\u3059\u308b\u6297\u539f\uff08bait\u30bf\u30f3\u30d1\u30af\u8cea\uff09\u304c\u76f8\u4e92\u4f5c\u7528\u3059\u308b\u5225\u306e\u30bf\u30f3\u30d1\u30af\u8cea\uff08prey\u30bf\u30f3\u30d1\u30af\u8cea\uff09\u3068\u306e\u53cd\u5fdc\u3092\u963b\u5bb3\u3057\u306a\u3044\u6761\u4ef6\u304c\u5fc5\u8981\u306b\u306a\u308a\u307e\u3059\u3002\u901a\u5e38\u3001\u6297\u539f\u3068\u6297\u4f53\u306e\u30a2\u30d5\u30a3\u30cb\u30c6\u30a3\u30fc\u306f\u9ad8\u3044\u305f\u3081\u3001\u4e2d\u6027\u4ed8\u8fd1\u306e\u4e00\u822c\u7684\u306a\u30d0\u30c3\u30d5\u30a1\u30fc\uff08PBS\u3084TBS\uff09\u304c\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u3057\u304b\u3057bait\u3068prey\u9593\u306e\u30a2\u30d5\u30a3\u30cb\u30c6\u30a3\u30fc\u306f\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u3088\u3063\u3066\u5927\u304d\u304f\u7570\u306a\u308b\u305f\u3081\u3001pH\u3084\u5869\u6fc3\u5ea6\u306e\u81f3\u9069\u6761\u4ef6\u3092\u691c\u8a0e\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u307e\u305f\u76f8\u4e92\u4f5c\u7528\u306b\u88dc\u56e0\u5b50\u304c\u5fc5\u8981\u306a\u5834\u5408\u306f\u7d50\u5408\u30d0\u30c3\u30d5\u30a1\u30fc\u306b\u88dc\u56e0\u5b50\u3092\u6dfb\u52a0\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u754c\u9762\u6d3b\u6027\u5264\u3084\u9084\u5143\u5264\u306e\u5b58\u5728\u306b\u3088\u308a\u53cd\u5fdc\u304c\u963b\u5bb3\u3055\u308c\u308b\u5834\u5408\u306b\u306f\u305d\u308c\u3089\u306e\u9664\u53bb\u3082\u5fc5\u8981\u306b\u306a\u308a\u307e\u3059\u3002<\/p>\n<h3>\u6d17\u6d44\u30d0\u30c3\u30d5\u30a1\u30fc<\/h3>\n<p>\u6d17\u6d44\u30d0\u30c3\u30d5\u30a1\u30fc\u306f\u3001<strong>\u6297\u539f\u6297\u4f53\u53cd\u5fdc\u3084\u30bf\u30f3\u30d1\u30af\u8cea\u9593\u76f8\u4e92\u4f5c\u7528\u3092\u7dad\u6301\u3057\u3064\u3064\u3001\u975e\u7279\u7570\u7684\u306b\u7d50\u5408\u3057\u3066\u3044\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u9664\u53bb\u3067\u304d\u308b\u30d0\u30c3\u30d5\u30a1\u30fc\u304c\u7406\u60f3\u7684<\/strong>\u3067\u3059\u3002\u901a\u5e38\u3001PBS\u3084TBS\u306e\u3088\u3046\u306a\u751f\u7406\u5b66\u7684\u6761\u4ef6\u306b\u8fd1\u3044\u30d0\u30c3\u30d5\u30a1\u30fc\u3092\u30d9\u30fc\u30b9\u3068\u3057\u3066\u3001\u5fc5\u8981\u306b\u5fdc\u3058\u3066\u754c\u9762\u6d3b\u6027\u5264\uff08\u4f8b\uff1a0.5-1% NP-40, Triton-X100, CHAPS\uff09\u3092\u6dfb\u52a0\u3057\u307e\u3059\u3002\u3082\u3057\u30bf\u30f3\u30d1\u30af\u8cea\u9593\u76f8\u4e92\u4f5c\u7528\u306e\u30a2\u30d5\u30a3\u30cb\u30c6\u30a3\u30fc\u304c\u6bd4\u8f03\u7684\u9ad8\u3051\u308c\u3070\u3001\u5869\u6fc3\u5ea6\u3092\u9ad8\u3081\u3066\u30a4\u30aa\u30f3\u7d50\u5408\u3084\u9759\u96fb\u6c17\u7684\u7d50\u5408\u3092\u89e3\u96e2\u3055\u305b\u308b\u3053\u3068\u3067\u3001\u975e\u7279\u7570\u7684\u306a\u7d50\u5408\u3092\u3057\u3066\u3044\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u9664\u53bb\u3092\u884c\u3046\u3053\u3068\u3082\u53ef\u80fd\u3067\u3059\uff08\u4f8b\uff1a0.5-1 M NaCl\uff09\u3002<\/p>\n<h3>\u6eb6\u51fa\u30d0\u30c3\u30d5\u30a1\u30fc<\/h3>\n<p>\u514d\u75ab\u6c88\u964d\u306b\u304a\u3051\u308b\u6eb6\u51fa\u3067\u306f\u3001\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306b\u3088\u308b\u691c\u51fa\u3092\u524d\u63d0\u3068\u3057\u3066\u3001<strong>SDS\u30b5\u30f3\u30d7\u30eb\u30d0\u30c3\u30d5\u30a1\u30fc\u3092\u5229\u7528\u3059\u308b\u3053\u3068\u3082\u53ef\u80fd<\/strong>\u3067\u3059\u3002SDS\u30b5\u30f3\u30d7\u30eb\u30d0\u30c3\u30d5\u30a1\u30fc\u3092\u5229\u7528\u3059\u308b\u3068\u3001\u5909\u6027\u3068\u9084\u5143\u4f5c\u7528\u306b\u3088\u308a\u52b9\u7387\u3088\u304f\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u56de\u53ce\u3067\u304d\u307e\u3059\u3002\u305f\u3060\u3057\u5909\u6027\u4f5c\u7528\u304c\u5927\u304d\u3044\u305f\u3081IP\u6297\u4f53\u306e\u518d\u5229\u7528\u304c\u3067\u304d\u306a\u3044\u70b9\u3084\u4e0b\u6d41\u306e\u5b9f\u9a13\u306b\u304a\u3051\u308b\u56de\u53ce\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5229\u7528\u65b9\u6cd5\u306b\u5236\u9650\u304c\u751f\u3058\u308b\u70b9\u304c\u30c7\u30e1\u30ea\u30c3\u30c8\u3067\u3059\u3002<\/p>\n<p><strong>\u5909\u6027\u4f5c\u7528\u306e\u5c11\u306a\u3044\u6eb6\u51fa\u30d0\u30c3\u30d5\u30a1\u30fc\u3068\u3057\u3066\u4e00\u822c\u7684\u306b\u5e83\u304f\u5229\u7528\u3055\u308c\u308b\u30d0\u30c3\u30d5\u30a1\u30fc\u304c0.1 M\u30b0\u30ea\u30b7\u30f3 pH2.5-3\u306e\u3088\u3046\u306a\u9178\u6027\u306e\u30d0\u30c3\u30d5\u30a1\u30fc<\/strong>\u3067\u3059\u3002\u3053\u306e\u30d0\u30c3\u30d5\u30a1\u30fc\u3067\u306f\u591a\u304f\u306e\u5834\u5408\u3001\u6297\u539f\u3068\u6297\u4f53\u9593\u306e\u7d50\u5408\u3092\u89e3\u96e2\u3055\u305b\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\uff08\u3053\u306e\u6761\u4ef6\u3067\u3082\u89e3\u96e2\u3057\u306a\u3044\u6297 \u539f\u6297\u4f53\u53cd\u5fdc\u3082\u3042\u308a\u307e\u3059\uff09\u3002\u6eb6\u51fa\u6db2\u306f\u3059\u3050\u306b\u4e2d\u548c\u30d0\u30c3\u30d5\u30a1\u30fc\uff081M Tris\u2022HCl, pH 8.5\u306a\u3069\uff09\u3068\u6df7\u5408\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u30bf\u30f3\u30d1\u30af\u8cea\u3078\u306e\u5f71\u97ff\u3092\u6291\u3048\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u3057\u304b\u3057\u306a\u304c\u3089\u3053\u306e\u6761\u4ef6\u3067\u3082\u77ed\u6642\u9593\u9178\u6027\u6761\u4ef6\u306b\u3055\u3089\u3055\u308c\u308b\u3053\u3068\u3067\u6eb6\u51fa\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u30c0\u30e1\u30fc\u30b8\u3092\u53d7\u3051\u308b\u3053\u3068\u3082\u3042\u308a\u307e\u3059\u3002<\/p>\n<p><strong>\u5909\u6027\u4f5c\u7528\u306e\u6700\u3082\u5c11\u306a\u3044\u6eb6\u51fa\u30d0\u30c3\u30d5\u30a1\u30fc\u3068\u3057\u3066\u3001\u5869\u6fc3\u5ea6\u306e\u9ad8\u3044\u30d0\u30c3\u30d5\u30a1\u30fc\u3082\u5229\u7528\u3067\u304d\u307e\u3059\u3002<\/strong>\u5f0a\u793e\u3067\u306f\u3001pH 6.6\u306e<a href=\"http:\/\/www.thermofisher.com\/order\/catalog\/product\/21027?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa29_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Gentle Ag\/Ab Elution Buffer<\/a>\u3092\u3054\u7528\u610f\u3057\u3066\u3044\u307e\u3059\u3002\u305f\u3060\u3057\u6eb6\u51fa\u52b9\u7387\u306fSDS\u30b5\u30f3\u30d7\u30eb\u30d0\u30c3\u30d5\u30a1\u30fc\u30840.1 M\u30b0\u30ea\u30b7\u30f3 pH2.5-3\u3088\u308a\u843d\u3061\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<p>\u305d\u306e\u4ed6\u3001\u3055\u307e\u3056\u307e\u306a\u6eb6\u51fa\u30d0\u30c3\u30d5\u30a1\u30fc\u304c\u77e5\u3089\u308c\u3066\u3044\u307e\u3059\uff08\u88682\uff09\u3002\u53cd\u5fdc\u7cfb\u3084\u4e0b\u6d41\u306e\u5b9f\u9a13\u306b\u5408\u308f\u305b\u3066\u3054\u691c\u8a0e\u304f\u3060\u3055\u3044\u3002<\/p>\n<table>\n<tbody>\n<tr>\n<td>\u5229\u7528\u3059\u308b\u6761\u4ef6<\/td>\n<td>\u4f8b<\/td>\n<\/tr>\n<tr>\n<td>\u4f4epH<\/td>\n<td><a href=\"http:\/\/www.thermofisher.com\/order\/catalog\/product\/21004?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa29_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">IgG Elution Buffer<\/a><br \/>\n100 mM Glycine\u2022HCl, pH 2.5-3.0<br \/>\n100 mM Citric acid, pH 3.0<\/td>\n<\/tr>\n<tr>\n<td>\u00a0\u9ad8pH<\/td>\n<td>50-100 mM Triethylamine \u307e\u305f\u306f Triethanolamine, pH 11.5<br \/>\n150 mM Ammonium hydroxide, pH 10.5<br \/>\n0.1 M Glycine\u2022NaOH, pH 10.0<\/td>\n<\/tr>\n<tr>\n<td>\u00a0\u30a4\u30aa\u30f3\u5f37\u5ea6<\/td>\n<td><a href=\"http:\/\/www.thermofisher.com\/order\/catalog\/product\/21027?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa29_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Gentle Ag\/Ab Elution Buffer<\/a><br \/>\n5 M Lithium chloride<br \/>\n3.5 M Magnesium \u307e\u305f\u306f Potassium chloride<br \/>\n3.0 M Potassium chloride<br \/>\n2.5 M Sodium \u307e\u305f\u306f Potassium iodide<br \/>\n0.2-3.0 M Sodium thiocyanate<br \/>\n0.1 M Tris-acetate with 2.0 M NaCl, pH 7.7<\/td>\n<\/tr>\n<tr>\n<td>\u00a0\u5909\u6027<\/td>\n<td>2-6 M Guanidine\u2022HCl<br \/>\n2-8 M Urea<br \/>\n1.0 M Ammonium thiocyanate<br \/>\n1% Sodium deoxycholate<br \/>\n1% SDS<\/td>\n<\/tr>\n<tr>\n<td>\u00a0\u6709\u6a5f\u6eb6\u5a92<\/td>\n<td>10% Dioxane<br \/>\n50% Ethylene glycol, pH 8-11.5<\/td>\n<\/tr>\n<tr>\n<td>\u00a0\u7af6\u5408\u5264<\/td>\n<td>&gt;0.1 M\u306e\u30ea\u30ac\u30f3\u30c9\u3084\u30a2\u30ca\u30ed\u30b0<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><span style=\"font-size: 8pt\">\u203bGentle Ag\/Ab Elution Buffer \u306f\u30ea\u30f3\u9178\u30d0\u30c3\u30d5\u30a1\u30fc\uff08\u7279\u306b\u30ea\u30f3\u9178\u30ab\u30ea\u30a6\u30e0\u3092\u542b\u3080\u30d0\u30c3\u30d5\u30a1\u30fc\uff09\u3068\u6df7\u5408\u3059\u308b\u3068\u4e0d\u6eb6\u6027\u306e\u6c88\u6bbf\u304c\u751f\u3058\u3066\u3057\u307e\u3044\u307e\u3059\u3002\u3057\u305f\u304c\u3063\u3066\uff08\u5171\uff09\u514d\u75ab\u6c88\u964d\uff08\u307e\u305f\u306f\u6297\u4f53\u7cbe\u88fd\uff09\u306b\u304a\u3044\u3066Gentle Ag\/Ab Elution Buffer\u3092\u4f7f\u7528\u3059\u308b\u5834\u5408\u306f\u3001\u7d50\u5408\u30d0\u30c3\u30d5\u30a1\u30fc\u3068\u3057\u3066\u975e\u30ea\u30f3\u9178\u30d0\u30c3\u30d5\u30a1\u30fc\u7cfb\u306e\u30d0\u30c3\u30d5\u30a1\u30fc\u307e\u305f\u306f\u5f0a\u793e\u306e<a href=\"http:\/\/www.thermofisher.com\/order\/catalog\/product\/21020?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa29_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Gentle Ag\/Ab Binding Buffer, pH 8.0<\/a>\u306e\u3054\u4f7f\u7528\u3092\u304a\u3059\u3059\u3081\u3057\u307e\u3059\u3002<\/span><\/p>\n<h2>\u3010\u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3011\u30bf\u30f3\u30d1\u30af\u8cea\u89e3\u6790\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u30cf\u30f3\u30c9\u30d6\u30c3\u30af<\/h2>\n<p>\u52b9\u7387\u7684\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u62bd\u51fa\u304b\u3089\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u89e3\u6790\u30c4\u30fc\u30eb\u307e\u3067\u3001\u5305\u62ec\u7684\u306b\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u3092\u7d39\u4ecb\u3057\u3066\u304a\u308a\u307e\u3059\u3002PDF\u30d5\u30a1\u30a4\u30eb\u306e\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3092\u3054\u5e0c\u671b\u306e\u65b9\u306f\u3001\u4e0b\u8a18\u30dc\u30bf\u30f3\u3088\u308a\u304a\u7533\u8fbc\u307f\u304f\u3060\u3055\u3044\u3002<\/p>\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/catalogs-protein-form.html?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa29_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9<\/a><\/p>\n<h2>\u5171\u514d\u75ab\u6c88\u964d\u6cd5\u95a2\u9023\u3001\u305d\u306e\u4ed6\u306e\u8a18\u4e8b\u306f\u3053\u3061\u3089<\/h2>\n<table style=\"border-color: lightgray;border-collapse: collapse;padding: 20px\" border=\"1\">\n<tbody>\n<tr>\n<td style=\"border-color: lightgray;padding: 20px\">\n<ul>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic8\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa29_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u7b2c8\u56de\u3011in vitro \u30bf\u30f3\u30d1\u30af\u8cea\u76f8\u4e92\u4f5c\u7528\u3092\u89e3\u6790\u3059\u308b\u65b9\u6cd5\u307e\u3068\u3081<br \/>\n<\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic9\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa29_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u7b2c9\u56de\u3011\u5171\u514d\u75ab\u6c88\u964d\u6cd5\u306e\u300c\u554f\u984c\u70b9\u300d\u3068\u300c\u6539\u5584\u7b56\u300d\u307e\u3068\u3081<br \/>\n<\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic12\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa29_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u7b2c12\u56de\u3011\u3055\u307e\u3056\u307e\u306a\u6297\u4f53\u7cbe\u88fd\u6cd5\u3092\u307e\u3068\u3081\u3066\u307f\u305f \u305d\u306e1<br \/>\n<\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic13\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa29_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u7b2c13\u56de\u3011\u3055\u307e\u3056\u307e\u306a\u6297\u4f53\u7cbe\u88fd\u6cd5\u3092\u307e\u3068\u3081\u3066\u307f\u305f \u305d\u306e2<br \/>\n<\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic14\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa29_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u7b2c14\u56de\u3011\u307f\u3093\u306a\u304c\u77e5\u308a\u305f\u3044\u30d7\u30ed\u30c6\u30a4\u30f3A\/G\/L\u306e\u6027\u8cea<br \/>\n<\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic21\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa29_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u7b2c21\u56de\u3011\u5171\u514d\u75ab\u6c88\u964d\u5b9f\u9a13\u306e\u57fa\u672c\u7684\u306a\u5b9f\u9a13\u624b\u9806<br \/>\n<\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic22\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa29_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u7b2c22\u56de\u3011\u5171\u514d\u75ab\u6c88\u964d\u5b9f\u9a13\u306b\u304a\u3051\u308b\u6297\u4f53\u306e\u56fa\u5b9a\u5316<\/a><\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u7528\u306b\u306f\u4f7f\u7528\u3044\u305f\u3060\u3051\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\uff08\u5171\uff09\u514d\u75ab\u6c88\u964d\u6cd5\u306f\u30bf\u30f3\u30d1\u30af\u8cea\u7cbe\u88fd\u3084\u30bf\u30f3\u30d1\u30af\u8cea\u30bf\u30f3\u30d1\u30af\u8cea\u76f8\u4e92\u4f5c\u7528\u89e3\u6790\u3092\u884c\u3046\u53e4\u5178\u7684\u306a\u624b\u6cd5\u3067\u3059\u304c\u3001\u73fe\u5728\u3067\u3082\u5e45\u5e83\u304f\u5229\u7528\u3055\u308c\u3066\u3044\u308b\u91cd\u8981\u306a\u6280\u8853\u3067\u3059\u3002\u4eca\u56de\u306f\u3001\u7d50\u5408\u30d0\u30c3\u30d5\u30a1\u30fc\u3001\u6d17\u6d44\u30d0\u30c3\u30d5\u30a1\u30fc\u3001\u6eb6\u51fa\u30d0\u30c3\u30d5\u30a1\u30fc\u306e\u5404\u7a2e\u30d0\u30c3\u30d5\u30a1\u30fc\u306b\u3064\u3044\u3066\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n","protected":false},"author":975,"featured_media":2401,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[156],"tags":[237,21],"division":[],"class_list":{"0":"post-258","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-mol-bio","8":"tag-protein-experiments","9":"tag-protein-analysis","10":"entry"},"_selected_authors":[],"_selected_reviewers":[],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.8 (Yoast SEO v27.8) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>\u5171\u514d\u75ab\u6c88\u964d\u5b9f\u9a13\u306b\u304a\u3051\u308b\u30d0\u30c3\u30d5\u30a1\u30fc\u306b\u3064\u3044\u3066\uff5c\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\uff01\u30bf\u30f3\u30d1\u30af\u8cea\u5b9f\u9a13\u3042\u308c\u3053\u308c \u7b2c23\u56de - Learning at the Bench<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic23\/\" \/>\n<meta property=\"og:locale\" content=\"ja_JP\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" 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