{"id":332,"date":"2024-03-08T00:00:00","date_gmt":"2024-03-07T15:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/digitalpcr1\/"},"modified":"2025-07-01T16:42:43","modified_gmt":"2025-07-01T07:42:43","slug":"digitalpcr1","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/digitalpcr1\/","title":{"rendered":"\u6b21\u4e16\u4ee3\u306e\u5b9a\u91cf\u6280\u8853\uff01\u30c7\u30b8\u30bf\u30ebPCR\u306e\u539f\u7406\u3068\u306f\uff1f"},"content":{"rendered":"<h2>\u30c7\u30b8\u30bf\u30ebPCR\u3068\u306f\uff1f<\/h2>\n<p>\u5f93\u6765\u306e\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306f\u3001\u30d1\u30ef\u30d5\u30eb\u3067\u611f\u5ea6\u306e\u9ad8\u3044\u907a\u4f1d\u5b50\u89e3\u6790\u6280\u8853\u3068\u3057\u3066\u3001\u5e45\u5e83\u3044\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u3067\u4f7f\u7528\u3055\u308c\u3066\u304d\u307e\u3057\u305f\u3002\u5897\u5e45\u53cd\u5fdc\u304c\u5b8c\u4e86\u3057\u3066\u304b\u3089\u30c7\u30fc\u30bf\u3092\u53ce\u96c6\u3059\u308b\u5f93\u6765\u306e\u30a8\u30f3\u30c9\u30dd\u30a4\u30f3\u30c8PCR\u6cd5\u3067\u306f\u3001\u6838\u9178\u306e\u958b\u59cb\u6fc3\u5ea6\u3092\u6e2c\u5b9a\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u305b\u3093\u3002\u4e00\u65b9\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306f\u305d\u306e\u540d\u304c\u793a\u3059\u3068\u304a\u308a\u3001PCR\u5897\u5e45\u3092\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0\u3067\u6e2c\u5b9a\u3057\u307e\u3059\u3002\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u767b\u5834\u306f\u3001PCR\u30d9\u30fc\u30b9\u306eDNA\u304a\u3088\u3073RNA\u5b9a\u91cf\u3092\u4e00\u5909\u3055\u305b\u307e\u3057\u305f\u3002<\/p>\n<p>\u30c7\u30b8\u30bf\u30ebPCR\u306f\u3001\u3053\u308c\u307e\u3067\u306b\u306a\u3044\u30a2\u30d7\u30ed\u30fc\u30c1\u3067\u6838\u9178\u306e\u691c\u51fa\u30fb\u5b9a\u91cf\u3092\u884c\u3044\u3001\u7d71\u8a08\u7684\u624b\u6cd5\u3092\u3068\u304a\u3057\u3066\u5206\u5b50\u306e\u7d76\u5bfe\u6570\u3092\u63a8\u91cf\u3057\u307e\u3059\u3002\u3053\u306e\u6280\u8853\u306f\u3001<strong>\u5404\u30b5\u30f3\u30d7\u30eb\u4e2d\u306e\u6838\u9178\u306e\u521d\u671f\u91cf\u3092\u6e2c\u5b9a\u3059\u308b\u969b\u306b\u3001\u5897\u5e45\u30b5\u30a4\u30af\u30eb\u6570\u306b\u4f9d\u5b58\u3059\u308b\u3053\u3068\u306a\u304f\u7d76\u5bfe\u5b9a\u91cf\u3084\u30ec\u30a2\u306a\u5bfe\u7acb\u907a\u4f1d\u5b50\u306e\u691c\u51fa\u3092\u884c\u3044\u3001\u5f93\u6765\u306e\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3068\u306f\u7570\u306a\u308b\u30a2\u30d7\u30ed\u30fc\u30c1\u3092\u63d0\u4f9b\u3057\u307e\u3059<\/strong>\u3002<\/p>\n<table>\n<caption>\u8868 \u30c7\u30b8\u30bf\u30ebPCR vs. \u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR vs. \u30a8\u30f3\u30c9\u30dd\u30a4\u30f3\u30c8PCR<\/caption>\n<tbody>\n<tr>\n<td><\/td>\n<td><strong>\u30c7\u30b8\u30bf\u30ebPCR<\/strong><\/td>\n<td><strong>\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR<\/strong><\/td>\n<td><strong>\u5f93\u6765\u306ePCR<\/strong><\/td>\n<\/tr>\n<tr>\n<td><strong>\u6982\u8981<\/strong><\/td>\n<td>\u5fae\u7d30\u306a\u30a6\u30a7\u30eb\u306b\u5206\u914d\u3057PCR\u3092\u884c\u3046\u3053\u3068\u3067\u30cd\u30ac\u30c6\u30a3\u30d6\u30a6\u30a7\u30eb\u306e\u53cd\u5fdc\u306e\u5272\u5408\u3092\u6e2c\u5b9a\u3057\u7d76\u5bfe\u6570\u3092\u5b9a\u91cf<\/td>\n<td>PCR\u306b\u3088\u308b\u6838\u9178\u306e\u5897\u5e45\u91cf\u3092\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0\u306b\u6e2c\u5b9a\u3057\u3001\u5897\u5e45\u7387\u306b\u57fa\u3065\u3044\u3066\u5b9a\u91cf<\/td>\n<td>PCR\u306b\u3088\u308b\u5897\u5e45\u7d42\u4e86\u6642\u306ePCR\u7523\u7269\u306e\u91cf\u3092\u6e2c\u5b9a<\/td>\n<\/tr>\n<tr>\n<td><strong>\u5b9a\u91cf\u7684\uff1f<\/strong><\/td>\n<td>\u306f\u3044 &#8211; \u30cd\u30ac\u30c6\u30a3\u30d6\u53cd\u5fdc\u306e\u5272\u5408\u3092\u30dd\u30a2\u30bd\u30f3\u7d71\u8a08\u30a2\u30eb\u30b4\u30ea\u30ba\u30e0\u306b\u5408\u308f\u305b\u3066\u5b9a\u91cf<\/td>\n<td>\u306f\u3044 &#8211; PCR\u7523\u7269\u306e\u91cf\u304c\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u6838\u9178\u306e\u91cf\u306b\u6b63\u6bd4\u4f8b\u3059\u308b\u3001PCR\u306e\u6307\u6570\u5897\u6b96\u30d5\u30a7\u30fc\u30ba\u4e2d\u306b\u30c7\u30fc\u30bf\u3092\u53ce\u96c6<\/td>\n<td>\u3044\u3044\u3048 &#8211; \u3057\u304b\u3057\u3001\u5897\u5e45\u3055\u308c\u305fPCR\u7523\u7269\u3068\u65e2\u77e5\u6fc3\u5ea6\u306e\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\u3092\u30b2\u30eb\u4e0a\u3067\u6bd4\u8f03\u3059\u308b\u3053\u3068\u3067\u3001\u534a\u5b9a\u91cf\u7684\u306a\u6e2c\u5b9a\u304c\u53ef\u80fd<\/td>\n<\/tr>\n<tr>\n<td><strong>\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3<\/strong><\/td>\n<td>\n<ul>\n<li>\u30a6\u30a4\u30eb\u30b9\u306e\u7d76\u5bfe\u5b9a\u91cf<\/li>\n<li>\u6838\u9178\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\u306e\u7d76\u5bfe\u5b9a\u91cf<\/li>\n<li>\u6b21\u4e16\u4ee3\u30b7\u30fc\u30b1\u30f3\u30b5\u306e\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u306e\u7d76\u5bfe\u5b9a\u91cf<\/li>\n<li>\u30ec\u30a2\u306a\u5909\u7570\u306e\u691c\u51fa<\/li>\n<li>\u907a\u4f1d\u5b50\u767a\u73fe\u5b9a\u91cf<\/li>\n<li>\u6df7\u5408\u7269\u306e\u6fc3\u7e2e\u3068\u5206\u96e2<\/li>\n<\/ul>\n<\/td>\n<td>\n<ul>\n<li>\u907a\u4f1d\u5b50\u767a\u73fe\u5b9a\u91cf<\/li>\n<li>\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u306e\u691c\u8a3c<\/li>\n<li>\u54c1\u8cea\u7ba1\u7406\u304a\u3088\u3073\u30a2\u30c3\u30bb\u30a4\u306e\u30d0\u30ea\u30c7\u30fc\u30b7\u30e7\u30f3<\/li>\n<li>\u75c5\u539f\u4f53\u306e\u691c\u51fa<\/li>\n<li>SNP\u30b8\u30a7\u30ce\u30bf\u30a4\u30d4\u30f3\u30b0<\/li>\n<li>\u30b3\u30d4\u30fc\u6570\u591a\u578b(CNV)<\/li>\n<li>\u30de\u30a4\u30af\u30edRNA\u89e3\u6790<\/li>\n<li>\u30a6\u30a4\u30eb\u30b9\u5b9a\u91cf<\/li>\n<li>siRNA\/RNAi\u306e\u89e3\u6790<\/li>\n<\/ul>\n<\/td>\n<td>\n<ul>\n<li>\u4e0b\u4f4d\u306e\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306e\u305f\u3081\u306eDNA\u306e\u5897\u5e45<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<tr>\n<td>\u30e1\u30ea\u30c3\u30c8<\/td>\n<td>\n<ul>\n<li>\u30ea\u30d5\u30a1\u30ec\u30f3\u30b9\u30b5\u30f3\u30d7\u30eb\u3084\u6a19\u6e96\u66f2\u7dda\u304c\u4e0d\u8981<\/li>\n<li>PCR\u306e\u5897\u5e45\u7523\u7269\u3092\u5897\u3084\u3059\u3053\u3068\u3067\u5fc5\u8981\u306a\u7cbe\u5ea6\u3092\u78ba\u4fdd\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd<\/li>\n<li>PCR\u53cd\u5fdc\u306b\u304a\u3051\u308b\u963b\u5bb3\u7269\u8cea\u306e\u5f71\u97ff\u3092\u53d7\u3051\u306b\u304f\u3044<\/li>\n<li>\u30b3\u30d4\u30fc\u6570\u306b\u5bfe\u3057\u3066\u30ea\u30cb\u30a2\u306a\u6e2c\u5b9a\u5024\u304c\u671f\u5f85\u3067\u304d\u3001\u6975\u308f\u305a\u304b\u306a\u500d\u7387\u5909\u5316\u306e\u9055\u3044\u3092\u691c\u51fa<\/li>\n<\/ul>\n<\/td>\n<td>\n<ul>\n<li>\u5e83\u3044\u30c0\u30a4\u30ca\u30df\u30c3\u30af\u30ec\u30f3\u30b8<\/li>\n<li>PCR\u5f8c\u306e\u51e6\u7406\u304c\u4e0d\u8981<\/li>\n<li>\u6fc3\u5ea6\u5dee1.5~2\u500d\u7a0b\u5ea6\u307e\u3067\u8b58\u5225\u53ef\u80fd<\/li>\n<li>PCR\u5897\u5e45\u4e2d\u306b\u30c7\u30fc\u30bf\u3092\u53ce\u96c6<\/li>\n<li>\u86cd\u5149\u30b7\u30b0\u30ca\u30eb\u306e\u5897\u52a0\u304c\u5897\u5e45\u3055\u308c\u305f\u30a2\u30f3\u30d7\u30ea\u30b3\u30f3\u6570\u306b\u6b63\u6bd4\u4f8b<\/li>\n<li>\u86cd\u5149\u30d7\u30ed\u30fc\u30d6\u306e\u5207\u65ad\u306b\u3088\u3063\u3066\u30a2\u30f3\u30d7\u30ea\u30b3\u30f3\u306e\u5897\u5e45\u91cf\u3092\u5b89\u5b9a\u7684\u306b\u691c\u51fa<\/li>\n<\/ul>\n<\/td>\n<td>\n<ul>\n<li>\u8a2d\u8a08\u304c\u30b7\u30f3\u30d7\u30eb<\/li>\n<li>\u4f7f\u7528\u304c\u6bd4\u8f03\u7684\u7c21\u5358<\/li>\n<li>\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u2015\u3068\u975e\u6a19\u8b58\u8a66\u85ac\u3067\u5229\u7528\u304c\u53ef\u80fd<\/li>\n<li>\u7279\u5225\u306a\u30c8\u30ec\u30fc\u30cb\u30f3\u30b0\u4e0d\u8981<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h2>\u30c7\u30b8\u30bf\u30ebPCR\u306e\u539f\u7406<\/h2>\n<p>\u5f93\u6765\u306e\u30a8\u30f3\u30c9\u30dd\u30a4\u30f3\u30c8PCR\u306f\u30d7\u30e9\u30c8\u30fc\u306a\u72b6\u614b\u3067\u6e2c\u5b9a\u3057\u307e\u3059\u304c\u3001\u53cd\u5fdc\u901f\u5ea6\u306e\u3070\u3089\u3064\u304d\u306b\u3088\u3063\u3066\u7d50\u679c\u306b\u3082\u3070\u3089\u3064\u304d\u304c\u751f\u3058\u307e\u3059\u3002\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306f\u3088\u308a\u6b63\u78ba\u306a\u5b9a\u91cf\u306e\u305f\u3081\u306b\u6307\u6570\u30d5\u30a7\u30fc\u30ba\u3067\u6e2c\u5b9a\u3057\u307e\u3059\u3002\u3053\u308c\u3089\u306b\u5bfe\u3057\u3066\u3001<strong>\u30c7\u30b8\u30bf\u30ebPCR\u306f\u30b5\u30f3\u30d7\u30eb\u306b\u542b\u307e\u308c\u308b\u500b\u3005\u306e\u5206\u5b50\u306e\u6570\u3092\u30ab\u30a6\u30f3\u30c8\u3057\u3066\u7d76\u5bfe\u5b9a\u91cf\u3092\u884c\u3044\u307e\u3059<\/strong>\u3002<\/p>\n<p>\u30c7\u30b8\u30bf\u30ebPCR\u3067\u306f\u3001<strong>\u591a\u6570\u306e\u30a6\u30a7\u30eb\u306bDNA\u3084cDNA\u30b5\u30f3\u30d7\u30eb\u3092\u5206\u914d\u3057\u3066\u3001\u500b\u3005\u306e\u30a6\u30a7\u30eb\u3067\u540c\u6642\u4e26\u884c\u306b\u30a8\u30f3\u30c9\u30dd\u30a4\u30f3\u30c8PCR\u3092\u884c\u3044\u307e\u3059\u30021\u3064\u3042\u308b\u3044\u306f\u8907\u6570\u306e\u30bf\u30fc\u30b2\u30c3\u30c8\u5206\u5b50\u3092\u542b\u3080\u30a6\u30a7\u30eb\u3082\u3042\u308c\u3070\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u5206\u5b50\u3092\u5168\u304f\u542b\u307e\u306a\u3044\u30a6\u30a7\u30eb\u304c\u5b58\u5728\u3057\u307e\u3059\u3002PCR\u7d42\u4e86\u5f8c\u306b\u30a6\u30a7\u30eb\u6bce\u306bPCR\u5897\u5e45\u306e\u6709\u7121\u3092\u89e3\u6790\u3057\u3001\u5897\u5e45\u304c\u3042\u3063\u305f\u30a6\u30a7\u30eb\u306f\u30bf\u30fc\u30b2\u30c3\u30c8\u5206\u5b50\u304c\u5165\u3063\u3066\u3044\u305f\u30dd\u30b8\u30c6\u30a3\u30d6\u53cd\u5fdc\u3068\u3057\u3066\u3001\u5897\u5e45\u304c\u306a\u304b\u3063\u305f\u30a6\u30a7\u30eb\u306f\u30bf\u30fc\u30b2\u30c3\u30c8\u3092\u542b\u307e\u306a\u3044\u30cd\u30ac\u30c6\u30a3\u30d6\u53cd\u5fdc\u3068\u3057\u3066\u6570\u3092\u30ab\u30a6\u30f3\u30c8\u3057\u307e\u3059<\/strong>\u3002<\/p>\n<p>\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u6d41\u4f53\u30d7\u30ec\u30fc\u30c8\u306f\u7c21\u4fbf\u3067\u30b7\u30f3\u30d7\u30eb\u306a\u4ed5\u7d44\u307f\u3067\u4f55\u5343\u3082\u306ePCR\u53cd\u5fdc\u3092\u540c\u6642\u4e26\u884c\u3067\u884c\u3046\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u5404\u30a6\u30a7\u30eb\u306b\u30b5\u30f3\u30d7\u30eb\u6df7\u5408\u6db2\u3084\u30de\u30b9\u30bf\u30fc\u30df\u30c3\u30af\u30b9\u304c\u5206\u914d\u3055\u308c\u3001\u500b\u3005\u306b\u30a8\u30f3\u30c9\u30dd\u30a4\u30f3\u30c8PCR\u304c\u884c\u308f\u308c\u307e\u3059\u3002<\/p>\n<p>PCR\u5f8c\u306b\u5404\u30a6\u30a7\u30eb\u306e\u30b7\u30b0\u30ca\u30eb\u306e\u6709\u7121\u3092\u691c\u51fa\u3057\u3001\u30cd\u30ac\u30c6\u30a3\u30d6\u53cd\u5fdc\u306e\u5272\u5408\u3092\u57fa\u306b\u3001\u30b5\u30f3\u30d7\u30eb\u4e2d\u306e\u30bf\u30fc\u30b2\u30c3\u30c8\u5206\u5b50\u306e\u7d76\u5bfe\u5b9a\u91cf\u3092\u884c\u3044\u307e\u3059\u3002<strong>\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\u3084\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u30b5\u30f3\u30d7\u30eb\u3068\u306e\u6bd4\u8f03\u306f\u5fc5\u8981\u3042\u308a\u307e\u305b\u3093<\/strong>\u3002<\/p>\n<p>\u30b5\u30f3\u30d7\u30eb\u4e2d\u306e\u30bf\u30fc\u30b2\u30c3\u30c8\u5206\u5b50\u306f\u30e9\u30f3\u30c0\u30e0\u306b\u5206\u914d\u3055\u308c\u308b\u305f\u3081\u3001\u30dd\u30b8\u30c6\u30a3\u30d6\u53cd\u5fdc\u3068\u3055\u308c\u305f\u30a6\u30a7\u30eb\u306b\u3001\uff11\u5206\u5b50\u306e\u307f\u5206\u914d\u3055\u308c\u305f\u3068\u3044\u3046\u78ba\u8a3c\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u305d\u3053\u3067\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u914d\u5217\u306e\u5206\u5b50\u304c\u8907\u6570\u5206\u914d\u3055\u308c\u305f\u53ef\u80fd\u6027\u306e\u3042\u308b\u30a6\u30a7\u30eb\u3092\u7b97\u51fa\u3059\u308b\u305f\u3081\u3001\u30dd\u30a2\u30bd\u30f3\u30e2\u30c7\u30eb\u3092\u7528\u3044\u3066\u3001\u88dc\u6b63\u4fc2\u6570\u3092\u9069\u7528\u3057\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u306e\u7d76\u5bfe\u6570\u3092\u7b97\u51fa\u3057\u307e\u3059\u3002<\/p>\n<div id=\"attachment_1036\" style=\"width: 927px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/dPCR1-fig1.png\" rel=\"attachment wp-att-1036\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-1036\" class=\"size-full wp-image-1036\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/dPCR1-fig1.png\" alt=\"dPCR1-fig1\" width=\"917\" height=\"286\" \/><\/a><p id=\"caption-attachment-1036\" class=\"wp-caption-text\">\u56f31 \u30c7\u30b8\u30bf\u30ebPCR\u306e\u539f\u7406\u3002\u30c7\u30b8\u30bf\u30ebPCR\u3092\u5b9f\u65bd\u3059\u308b\u969b\u306b\u306f\u3001\u6838\u9178\u306e\u6df7\u5408\u7269\u3092\u3001\u3042\u308b\u30a6\u30a7\u30eb\u306f1\u500b\u306e\u30bf\u30fc\u30b2\u30c3\u30c8\u5206\u5b50\u3092\u542b\u3080\u4e00\u65b9\u3001\u4ed6\u306e\u30a6\u30a7\u30eb\u306b\u306f\u30bf\u30fc\u30b2\u30c3\u30c8\u3092\u542b\u307e\u306a\u3044\u7a0b\u5ea6\u306b\u591a\u6570\u306e\u53cd\u5fdc\u30a6\u30a7\u30eb\u306b\u5206\u914d\u3057\u307e\u3059\u3002\u5404\u53cd\u5fdc\u6db2\u3067\u901a\u5e38\u306ePCR\u3092\u5b9f\u65bd\u3057\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u5206\u5b50\u3092\u542b\u307e\u306a\u3044\u30a6\u30a7\u30eb\u3092\u540c\u5b9a\u3057\u307e\u3059\u3002\u6a19\u6e96\u7684\u306a\u7d71\u8a08\u30e2\u30c7\u30eb\u3067\u88dc\u6b63\u8a08\u7b97\u3092\u884c\u3044\u6700\u7d42\u7684\u306a\u6fc3\u5ea6\u306e\u5024\u3092\u5f97\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u30c7\u30b8\u30bf\u30ebPCR\u3067\u306f\u30b3\u30d4\u30fc\u6570\u306e\u5b9a\u91cf\u306bCt\u5024\u3092\u4f7f\u7528\u3057\u306a\u3044\u306e\u3067\u3001\u7d76\u5bfe\u5b9a\u91cf\u306b\u304a\u3044\u3066\u65e2\u77e5\u306e\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\u3068\u306e\u6bd4\u8f03\u304c\u4e0d\u8981\u3068\u306a\u308a\u307e\u3059\u3002<\/p><\/div>\n<p>\u30dd\u30a2\u30bd\u30f3\u30e2\u30c7\u30eb\u3092\u9069\u7528\u3059\u308b\u306b\u306f\u3001\u5c11\u306a\u304f\u3068\u30821\u3064\u306e\u30cd\u30ac\u30c6\u30a3\u30d6\u53cd\u5fdc\uff08\u30bf\u30fc\u30b2\u30c3\u30c8\u5206\u5b50\u3092\u542b\u307e\u306a\u3044\u30a6\u30a7\u30eb\uff09\u304c\u5fc5\u8981\u3067\u3059\u3002\u30dd\u30a2\u30bd\u30f3\u30e2\u30c7\u30eb\u306f\u3001\u30b3\u30d4\u30fc\u6570\u304c1\u3064\u306e\u30a6\u30a7\u30eb\u306b\u30bc\u30ed\u30011\u30012\u3042\u308b\u3044\u306f3\u30b3\u30d4\u30fc\u5165\u308b\u5834\u5408\u306e\u78ba\u7387\u3092\u8868\u3057\u307e\u3059\u3002<strong>\u30dd\u30ef\u30bd\u30f3\u30e2\u30c7\u30eb\u3088\u308a\u8907\u6570\u306e\u30bf\u30fc\u30b2\u30c3\u30c8\u5206\u5b50\u3092\u542b\u3080\u53cd\u5fdc\u3092\u88dc\u6b63\u3057\u3001\u30b5\u30f3\u30d7\u30eb\u4e2d\u306b\u542b\u307e\u308c\u308b\u6b63\u3057\u3044\u30bf\u30fc\u30b2\u30c3\u30c8\u5206\u5b50\u306e\u6570\u3092\u7b97\u51fa\u3057\u307e\u3059<\/strong>\u3002\u30b5\u30f3\u30d7\u30eb\u306e\u5206\u914d\u3068\u30dd\u30a2\u30bd\u30f3\u7d71\u8a08\u30c7\u30fc\u30bf\u89e3\u6790\u306e\u7d44\u307f\u5408\u308f\u305b\u306b\u3088\u3063\u3066\u3001<strong>\u5f93\u6765\u306e\u30a8\u30f3\u30c9\u30dd\u30a4\u30f3\u30c8PCR\u3084\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u6cd5\u3088\u308a\u3082\u9ad8\u3044\u5b9a\u91cf\u7cbe\u5ea6\u3092\u5b9f\u73fe\u3057\u307e\u3059<\/strong>\u3002<br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/dPCR1-fig2.png\" rel=\"attachment wp-att-1037\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-1037\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/dPCR1-fig2.png\" alt=\"dPCR1-fig2\" width=\"469\" height=\"271\" \/><\/a><br \/>\n\u30dd\u30a2\u30bd\u30f3\u30e2\u30c7\u30eb\u3092\u4f7f\u7528\u3059\u308b\u3068\u304d\u306b\u3001\u6700\u3082\u91cd\u8981\u306a\u306e\u306f\u30cd\u30ac\u30c6\u30a3\u30d6\u53cd\u5fdc\u6570\u3067\u3059\uff08\u4e0a\u56f3\u306e96\u30a6\u30a7\u30eb\u30d7\u30ec\u30fc\u30c8\u306e\u7d50\u679c\u53c2\u7167\uff09\u3002<strong>\u30cd\u30ac\u30c6\u30a3\u30d6\u53cd\u5fdc\u6570\u306b\u57fa\u3065\u3044\u3066\u3001\u30aa\u30ea\u30b8\u30ca\u30eb\u306e\u30b5\u30f3\u30d7\u30eb\u4e2d\u306eDNA\u5206\u5b50\u306e\u6570\u3092\u5b9a\u91cf\u3057\u3001PCR\u89e3\u6790\u5f8c\u306e\u30dd\u30b8\u30c6\u30a3\u30d6\u53cd\u5fdc\u3068\u30cd\u30ac\u30c6\u30a3\u30d6\u53cd\u5fdc\u306e\u6bd4\u7387\u3092\u5b9a\u3081\u307e\u3059<\/strong>\u3002<\/p>\n<p>\u30c7\u30b8\u30bf\u30ebPCR\u306f\u3001<strong>\u30ec\u30a2\u306a\u5909\u7570\u306e\u691c\u51fa\u3001\u30b3\u30d4\u30fc\u6570\u591a\u578b\uff08CNV\uff09\u89e3\u6790\u3001\u30ec\u30a2\u306a\u907a\u4f1d\u5b50\u767a\u73fe\u89e3\u6790\u306a\u3069\u3001\u521d\u671f\u306e\u6838\u9178\u91cf\u304c\u5c11\u306a\u3044\u5834\u5408\u306e\u691c\u51fa\u3084\u3001\u3088\u308a\u9ad8\u3044\u7cbe\u5ea6\u3067\u30b5\u30f3\u30d7\u30eb\u9593\u306e\u30bf\u30fc\u30b2\u30c3\u30c8\u91cf\u306e\u6bd4\u8f03\u304c\u5fc5\u8981\u3068\u3055\u308c\u308b\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306b\u9069\u3057\u3066\u3044\u307e\u3059<\/strong>\u3002<\/p>\n<div id=\"attachment_1038\" style=\"width: 1232px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/dPCR1-fig3.png\" rel=\"attachment wp-att-1038\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-1038\" class=\"size-full wp-image-1038\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/dPCR1-fig3.png\" alt=\"dPCR1-fig3\" width=\"1222\" height=\"417\" \/><\/a><p id=\"caption-attachment-1038\" class=\"wp-caption-text\">\u56f33 \u30c7\u30b8\u30bf\u30ebPCR\u306f1\u30a6\u30a7\u30eb\u3042\u305f\u308a1.6\u30b3\u30d4\u30fc\u306e\u3068\u304d\u306b\u6700\u5927\u9650\u306e\u7cbe\u5ea6\u304c\u5f97\u3089\u308c\u307e\u3059\u3002\u672c\u30b0\u30e9\u30d5\u306f\u300120,000\uff08\u7dd1\uff09\u300112.000\uff08\u9752\uff09\u304a\u3088\u30733,000\uff08\u8d64\uff09\u30a6\u30a7\u30eb\u3092\u7528\u3044\u3066\u30c7\u30b8\u30bf\u30ebPCR\u3092\u884c\u3063\u305f\u5834\u5408\u306e\u5230\u9054\u3059\u308b\u7cbe\u5ea6\u3092\u793a\u3057\u307e\u3059\u3002X\u8ef8\u306f\u30cd\u30ac\u30c6\u30a3\u30d6\u53cd\u5fdc\u306e\u5272\u5408\u3092\u793a\u3057\uff08\u30b0\u30e9\u30d5\u4e2d\u306e\u7e26\u7dda\u306f20.32%\u3092\u793a\u3059\uff09\u3001Y\u8ef8\u306f\u7cbe\u5ea6\uff08\u4fe1\u983c\u533a\u959395%\uff09\u3092\u793a\u3057\u307e\u3059\u3002\u6700\u9ad8\u306e\u7cbe\u5ea6\u306f\u3001\u30cd\u30ac\u30c6\u30a3\u30d6\u53cd\u5fdc\u304c20%\u3001\u30dd\u30b8\u30c6\u30a3\u30d6\u53cd\u5fdc\u304c80%\u3067\u751f\u3058\u307e\u3059\u3002\u9752\u304a\u3088\u3073\u7dd1\u306e\u30e9\u30a4\u30f3\u3092\u898b\u308b\u3068\u300120,000\u30a6\u30a7\u30eb\u3001\u304a\u3088\u307312,000\u30a6\u30a7\u30eb\u306b\u304a\u3044\u3066\u3001\u30cd\u30ac\u30c6\u30a3\u30d6\u53cd\u5fdc\u306f5\u304b\u308990%\u306e\u9593\u3067\u7cbe\u5ea6\u306f\u4e00\u5b9a\u3057\u3066\u3044\u307e\u3059\u3002\u3053\u308c\u304c\u30c7\u30b8\u30bf\u30ebPCR\u3067\u89e3\u6790\u3059\u308b\u7406\u60f3\u7684\u306a\u30b5\u30f3\u30d7\u30eb\u6fc3\u5ea6\u306e\u7bc4\u56f2\u3067\u3059\uff08\u4f8b\u3048\u3070\u300115\u03bcL\u306e\u53cd\u5fdc\u6db2\u309220,000\u30a6\u30a7\u30eb\u306b\u5206\u914d\u3057\u3066\u30011\u30a6\u30a7\u30eb\u3042\u305f\u308a1\u30b3\u30d4\u30fc\u306b\u306a\u308b\u6fc3\u5ea6\uff09\u3002\u4e00\u65b9\u4e21\u6975\u306b\u304a\u3044\u3066\u306f\u3001\u7cbe\u5ea6\u306f\u6025\u6fc0\u306b\u843d\u3061\u307e\u3059\u3002<\/p><\/div>\n<h2>\u9ad8\u3044\u7cbe\u5ea6\u3068\u518d\u73fe\u6027\u3092\u5b9f\u73fe\u3059\u308b\u30c7\u30b8\u30bf\u30ebPCR<\/h2>\n<p>\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306e\u8a71\u306b\u5165\u308b\u524d\u306b\u3001\u3053\u3053\u3067<strong>\u30c7\u30b8\u30bf\u30ebPCR\u306e\u7cbe\u5ea6\u3068\u518d\u73fe\u6027\u3092\u793a\u3059\u30c7\u30fc\u30bf<\/strong>\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n<p>\u5de6\u306e\u30b0\u30e9\u30d5\u306f\u3001\u7d76\u5bfe\u5b9a\u91cf\u306b\u304a\u3044\u3066\u3001\u4ed6\u306e\u624b\u6cd5\u3068\u6bd4\u8f03\u3057\u305f\u30c7\u30b8\u30bf\u30ebPCR\u306e\u7cbe\u5ea6\u3092\u793a\u3057\u3066\u3044\u307e\u3059\u3002\u53f3\u306e\u30b0\u30e9\u30d5\u306f\u30014\u4eba\u306e\u5225\u3005\u306e\u30e6\u30fc\u30b6\u30fc\u304c1\u4eba10\u500b\u3001\u8a0840\u500b\u306e\u30c1\u30c3\u30d7\u306b\u540c\u3058\u30b5\u30f3\u30d7\u30eb\u3092\u30ed\u30fc\u30c7\u30a3\u30f3\u30b0\u3057\u305f\u6e2c\u5b9a\u7d50\u679c\u3092\u793a\u3057\u3066\u3044\u307e\u3059\u3002\u30b0\u30e9\u30d5\u304b\u3089\u308f\u304b\u308b\u3088\u3046\u306b\u3001<strong>4\u4eba\u5225\u3005\u306e\u30e6\u30fc\u30b6\u30fc\u3067\u3042\u3063\u3066\u3082\u57fa\u672c\u7684\u306b\u540c\u3058\u7d50\u679c\u3092\u5f97\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3057\u305f(\u56f34)<\/strong>\u3002<\/p>\n<div id=\"attachment_1039\" style=\"width: 721px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/dPCR1-fig4.png\" rel=\"attachment wp-att-1039\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-1039\" class=\"size-full wp-image-1039\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/dPCR1-fig4.png\" alt=\"dPCR1-fig4\" width=\"711\" height=\"356\" \/><\/a><p id=\"caption-attachment-1039\" class=\"wp-caption-text\">\u56f34 \u6e2c\u5b9a\u7d50\u679c\u306f\u4ed6\u306e\u30c6\u30af\u30ce\u30ed\u30b8\u30fc\u3068\u4e00\u81f4(\u5de6\u56f3)\u3002\u6e2c\u5b9a\u7d50\u679c\u306f\u30ec\u30d7\u30ea\u30b1\u30fc\u30c8\u9593\u3001\u30e6\u30fc\u30b6\u30fc\u9593\u3067\u4e00\u81f4(\u53f3\u56f3)\u3002<\/p><\/div>\n<h2>\u5e45\u5e83\u3044\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306b\u5bfe\u5fdc\u3059\u308b\u30c7\u30b8\u30bf\u30ebPCR<\/h2>\n<p>\u30c7\u30b8\u30bf\u30ebPCR\u306f\u3001<strong>\u30ec\u30a2\u306a\u5909\u7570\u306e\u691c\u51fa\u3001\u907a\u4f1d\u5b50\u767a\u73fe\u306e\u7d76\u5bfe\u5b9a\u91cf\u3001\u30a6\u30a4\u30eb\u30b9\u306e\u529b\u4fa1\u6e2c\u5b9a\u3001\u6b21\u4e16\u4ee3\u30b7\u30fc\u30b1\u30f3\u30b5\u306e\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u5b9a\u91cf\u3001\u6838\u9178\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\u306e\u4f5c\u88fd\u3001\u6df7\u5408\u7269\u306e\u6fc3\u7e2e\u3068\u5206\u96e2\u306b\u9069\u3057\u3066\u3044\u307e\u3059<\/strong>\u3002<\/p>\n<h3>\u5177\u4f53\u7684\u306a\u7d76\u5bfe\u5b9a\u91cf\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3<\/h3>\n<ul>\n<li><a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/pcr\/digital-pcr\/cell-gene-therapy-manufacturing.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_oc_s00_qpcr61_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u907a\u4f1d\u5b50\u6cbb\u7642\u306b\u304a\u3051\u308b\u30a6\u30a4\u30eb\u30b9\u529b\u4fa1\u306e\u6b63\u78ba\u306a\u6e2c\u5b9a<\/a> \u2013 AAV\u30d9\u30af\u30bf\u30fc\u3001\u7d30\u83cc\u6c5a\u67d3\u3001\u304a\u3088\u3073\u5bbf\u4e3b\u7d30\u80de\u306e\u6b8b\u7559DNA\u306e\u6b63\u78ba\u306a\u5b9a\u91cf\u304c\u53ef\u80fd<\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/pcr\/digital-pcr\/wastewater-surveillance.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_oc_s00_qpcr61_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30c7\u30b8\u30bf\u30ebPCR\u3092\u4f7f\u7528\u3057\u305f\u4e0b\u6c34\u30b5\u30fc\u30d9\u30a4\u30e9\u30f3\u30b9<\/a> \u2013 \u4e0b\u6c34\u30b5\u30f3\u30d7\u30eb\u304b\u3089\u306e\u30a6\u30a4\u30eb\u30b9\u7d76\u5bfe\u5b9a\u91cf<\/li>\n<li>\u30b3\u30d4\u30fc\u6570\u591a\u578b(CNV) \u2013 \u30b3\u30d4\u30fc\u6570\u306e\u308f\u305a\u304b\u306a\u9055\u3044\u3092\u9ad8\u7cbe\u5ea6\u3067\u691c\u51fa\u30fb\u5b9a\u91cf<\/li>\n<li>\u6b21\u4e16\u4ee3\u30b7\u30fc\u30b1\u30f3\u30b5\uff08NGS\uff09\u306e\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u5b9a\u91cf \u2013 \u30ea\u30d5\u30a1\u30ec\u30f3\u30b9\u30b5\u30f3\u30d7\u30eb\u304c\u306a\u304f\u3066\u3082NGS\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u306e\u7d76\u5bfe\u5b9a\u91cf\u3084\u30b7\u30fc\u30b1\u30f3\u30b9\u5f8c\u306e\u7d50\u679c\u3092\u691c\u8a3c<\/li>\n<li>\u30a6\u30a4\u30eb\u30b9\u91cf\u306e\u5b9a\u91cf \u2013 \u30d0\u30af\u30c6\u30ea\u30a2\u3084\u30a6\u30a4\u30eb\u30b9\u91cf\u306e\u7d76\u5bfe\u5b9a\u91cf\u3001\u75c5\u539f\u83cc\u306e\u7d76\u5bfe\u6570\u30ab\u30a6\u30f3\u30c8<\/li>\n<\/ul>\n<h3>\u5177\u4f53\u7684\u306a\u5bfe\u7acb\u907a\u4f1d\u5b50\u691c\u51fa\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3<\/h3>\n<ul>\n<li><a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/pcr\/digital-pcr\/mutation-detection.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_oc_s00_qpcr61_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30c7\u30b8\u30bf\u30ebPCR\u3092\u7528\u3044\u305f\u5909\u7570\u691c\u51fa<\/a> \u2013 \u304c\u3093\u7814\u7a76\u3084\u81e8\u5e8a\u30b5\u30f3\u30d7\u30eb\u306a\u3069\u306e\u5c11\u91cf\u306e\u30b5\u30f3\u30d7\u30eb\u3092\u7528\u3044\u305f\u30ec\u30a2\u306a\u5909\u7570\u306e\u691c\u51fa\u30fb\u5b9a\u91cf<\/li>\n<li>\u9ad8\u611f\u5ea6\u306aGMO\u691c\u51fa \u2013 \u690d\u7269\u306e\u5909\u7570\u3084\u907a\u4f1d\u5b50\u7d44\u307f\u63db\u3048\u751f\u7269\u306e\u9ad8\u611f\u5ea6\u306a\u691c\u51fa\u30fb\u7d76\u5bfe\u5b9a\u91cf<\/li>\n<\/ul>\n<h2>\u307e\u3068\u3081<\/h2>\n<p>\u30c7\u30b8\u30bf\u30ebPCR\u306e\u30e1\u30ea\u30c3\u30c8\u3092\u7c21\u5358\u306b\u307e\u3068\u3081\u3066\u307f\u307e\u3057\u3087\u3046\u3002\u307e\u305a\u3001<strong>\u30c7\u30b8\u30bf\u30ebPCR\u306f\u7d76\u5bfe\u5b9a\u91cf\u3092\u884c\u3046\u4ed5\u7d44\u307f<\/strong>\u3092\u63d0\u4f9b\u3057\u307e\u3059\u3002\u3053\u308c\u306b\u3088\u308a\u3001<strong>\u6a19\u6e96\u66f2\u7dda\u3092\u5fc5\u8981\u3068\u305b\u305a\u3001\u30b5\u30f3\u30d7\u30eb\u4e2d\u306e\u5206\u5b50\u306e\u6570\u3092\u5224\u65ad<\/strong>\u3067\u304d\u307e\u3059\u3002\u3053\u308c\u306f\u3001<strong>\u30ea\u30d5\u30a1\u30ec\u30f3\u30b9\u30b5\u30f3\u30d7\u30eb\u3092\u7528\u610f\u3067\u304d\u306a\u3044\u30b5\u30f3\u30d7\u30eb\u306e\u907a\u4f1d\u5b50\u767a\u73fe\u89e3\u6790\u306b\u304a\u3044\u3066\u7279\u306b\u6709\u7528<\/strong>\u3067\u3059\u3002<\/p>\n<p>2\u3064\u76ee\u3068\u3057\u3066\u3001\u30c7\u30b8\u30bf\u30ebPCR\u306f\u3001<strong>\u6b63\u5e38\u30b5\u30f3\u30d7\u30eb\u4e2d\u306b\u308f\u305a\u304b\u306b\u542b\u307e\u308c\u308b\u5909\u7570\u907a\u4f1d\u5b50\u306e\u5e0c\u5c11\u306a\u5206\u5b50\u3092\u540c\u5b9a<\/strong>\u3067\u304d\u307e\u3059\u3002\u4f8b\u3048\u3070\u3001FFPE\u5207\u7247\u306e\u816b\u760d\u30b5\u30f3\u30d7\u30eb\u306b\u304a\u3051\u308b\u30d7\u30ed\u30c8\u30aa\u30f3\u30b3\u30b8\u30fc\u30f3\u306e\u5909\u7570\u5bfe\u7acb\u907a\u4f1d\u5b50\u3001\u9aa8\u9ac4\u79fb\u690d\u30b5\u30f3\u30d7\u30eb\u306b\u304a\u3051\u308b\u30ad\u30e1\u30e9\u3001\u5faa\u74b0\u8840\u4e2d\u904a\u96e2DNA\uff08cfDNA\uff09\u306b\u304a\u3051\u308b\u767a\u304c\u3093\u6027\u5909\u7570\u306e\u691c\u51fa\u306a\u3069\u304c\u6319\u3052\u3089\u308c\u307e\u3059\u3002<\/p>\n<p>\u6700\u5f8c\u306b\u3001\u74b0\u5883\u4e0b\u306b\u5b58\u5728\u3059\u308bSDS\u3084\u30d8\u30d1\u30ea\u30f3\u3068\u3044\u3063\u305fPCR\u963b\u5bb3\u7269\u8cea\u306fPCR\u52b9\u7387\u306b\u5f71\u97ff\u3092\u4e0e\u3048\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u304c\u3001<strong>\u30c7\u30b8\u30bf\u30ebPCR\u306f\u30a8\u30f3\u30c9\u30dd\u30a4\u30f3\u30c8PCR\u53cd\u5fdc\u306e\u305f\u3081\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3088\u308a\u3082\u963b\u5bb3\u7269\u8cea\u306b\u5bfe\u3057\u3066\u8010\u6027\u304c\u3042\u308a\u307e\u3059<\/strong>\u3002\u3053\u308c\u306f\u3001\u74b0\u5883\u30b5\u30f3\u30d7\u30eb\u3084\u8cb4\u91cd\u306a\u304c\u3093\u30b5\u30f3\u30d7\u30eb\u3092\u6271\u3046\u7814\u7a76\u5ba4\u306b\u3068\u3063\u3066\u306f\u3001\u7279\u306b\u91cd\u8981\u306a\u30dd\u30a4\u30f3\u30c8\u3067\u3059\u3002<\/p>\n<h2>\u30c7\u30b8\u30bf\u30ebPCR\u306b\u3064\u3044\u3066\u3055\u3089\u306b\u8a73\u3057\u304f<\/h2>\n<p><a href=\"http:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/pcr\/real-time-pcr\/qpcr-education\/qpcr-vs-digital-pcr-vs-traditional-pcr.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_oc_s00_qpcr61_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3068\u30c7\u30b8\u30bf\u30ebPCR\u304a\u3088\u3073PCR\u306e\u6bd4\u8f03<\/a>\u3067\u7b2c3\u4e16\u4ee3\u306ePCR\u6280\u8853\u3092\u6bd4\u8f03\u3057\u3066\u307f\u3066\u304f\u3060\u3055\u3044\u3002\u65b0\u3057\u3044<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/pcr\/real-time-pcr\/real-time-pcr-instruments\/quantstudio-systems\/models\/quantstudio-absolute-q.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_oc_s00_qpcr61_Social_LAB\" target=\"_blank\" rel=\"noopener\">Applied Biosystems\u2122 QuantStudio\u2122 Absolute Q\u2122\u30c7\u30b8\u30bf\u30ebPCR\u30b7\u30b9\u30c6\u30e0<\/a>\u3092\u3088\u308a\u8a73\u3057\u304f\u77e5\u3063\u3066\u3044\u305f\u3060\u304f\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u672c\u30b7\u30b9\u30c6\u30e0\u306f\u3001\u5c02\u7528\u30d7\u30ec\u30fc\u30c8\u306b\u53cd\u5fdc\u6db2\u3092\u30a2\u30d7\u30ed\u30a4\u3059\u308b\u3060\u3051\u3067\u3001\u7c21\u5358\u306b\u30b9\u30bf\u30fc\u30c8\u3067\u304d\u307e\u3059\u3002\u30b5\u30f3\u30d7\u30eb\u306e\u79fb\u3057\u66ff\u3048\u3084\u8907\u96d1\u306a\u8a2d\u5b9a\u304c\u4e0d\u8981\u306a\u306e\u3067\u3001\u8ab0\u306b\u3067\u3082\u7c21\u5358\u306b\u64cd\u4f5c\u3057\u3066\u3044\u305f\u3060\u3051\u307e\u3059\u3002\u5b9f\u9a13\u3092\u59cb\u3081\u308b\u3068\u304d\u306b\u5f79\u7acb\u3064\u5c02\u9580\u7684\u60c5\u5831\u3001\u30b3\u30c4\u3084\u30d2\u30f3\u30c8\u3001\u3088\u304f\u3042\u308b\u6a5f\u5668\u30fb\u30bd\u30d5\u30c8\u30a6\u30a8\u30a2\u30a8\u30e9\u30fc\u306b\u5bfe\u3059\u308b\u89e3\u6c7a\u7b56\u306a\u3069\u3092\u3001\u30aa\u30f3\u30e9\u30a4\u30f3\u4e0a\u306e\u3053\u3061\u3089\u306e\u30b5\u30a4\u30c8\u3001<a href=\"http:\/\/www.thermofisher.com\/jp\/ja\/home\/technical-resources\/technical-reference-library\/real-time-digital-PCR-instruments-support-center.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_oc_s00_qpcr61_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">dPCR\u304a\u3088\u3073qPCR\u6a5f\u5668\u30b5\u30dd\u30fc\u30c8\u30bb\u30f3\u30bf\u30fc<\/a>\u3067\u4e00\u5ea6\u306b\u3054\u89a7\u3044\u305f\u3060\u304f\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n<h3>\u3010\u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3011\u30c7\u30b8\u30bf\u30ebPCR\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u30ac\u30a4\u30c9<\/h3>\n<p>\u30c7\u30b8\u30bf\u30ebPCR\u306b\u3088\u308b\u5b9a\u91cf\u304c\u6709\u7528\u306a\u8907\u6570\u306e\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306b\u3064\u3044\u3066\u7d39\u4ecb\u3057\u3066\u3044\u307e\u3059\u3002\u3053\u308c\u304b\u3089\u30c7\u30b8\u30bf\u30ebPCR\u5b9f\u9a13\u3092\u8003\u3048\u3066\u3044\u308b\u65b9\u306b\u6700\u9069\u3067\u3059\u3002<\/p>\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/life-science\/dpcr-applications-ebook-jp.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_oc_s00_qpcr61_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u306f\u3053\u3061\u3089<\/a><\/p>\n<h3>AbsoluteQ\u3092\u30d0\u30fc\u30c1\u30e3\u30eb\u30c7\u30e2\u3067\u4f53\u9a13\u3057\u3066\u307f\u307e\u305b\u3093\u304b<\/h3>\n<p>3D\u6280\u8853\u3092\u4f7f\u3063\u3066\u30b5\u30f3\u30d7\u30eb\u30ed\u30fc\u30c9\u3084\u30d7\u30ec\u30fc\u30c8\u30bb\u30c3\u30c8\u306a\u3069\u306e\u3001QuantStudio Absolute Q\u30c7\u30b8\u30bf\u30ebPCR\u30b7\u30b9\u30c6\u30e0\u306e\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u3092\u7591\u4f3c\u7684\u306b\u4f53\u9a13\u3067\u304d\u307e\u3059\u3002<\/p>\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/absolute-q-demo-form.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_oc_s00_qpcr61_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u304a\u7533\u3057\u8fbc\u307f\u306f\u3053\u3061\u3089<\/a><\/p>\n<p>\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u7528\u306b\u306f\u4f7f\u7528\u3044\u305f\u3060\u3051\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u6b21\u4e16\u4ee3\u306e\u5b9a\u91cf\u6280\u8853\u3068\u3057\u3066\u30016\u56de\u306e\u30b7\u30ea\u30fc\u30ba\u3067\u30c7\u30b8\u30bf\u30ebPCR\u306e\u6280\u8853\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002\u307e\u305a\u3001\u30c7\u30b8\u30bf\u30ebPCR (dPCR) \u306b\u3064\u3044\u3066\u6982\u8981\u3092\u3054\u7d39\u4ecb\u3057\u3001\u30c7\u30b8\u30bf\u30ebPCR\u306e\u3069\u3053\u304c\u6b21\u4e16\u4ee3\u306e\u6280\u8853\u306a\u306e\u304b\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR (qPCR)\u306a\u3069\u306e\u5f93\u6765\u306ePCR\u3068\u4f55\u304c\u9055\u3046\u306e\u304b\u306b\u3064\u3044\u3066\u3054\u8aac\u660e\u3057\u307e\u3059\u3002<\/p>\n","protected":false},"author":988,"featured_media":16536,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[156],"tags":[44],"division":[],"class_list":{"0":"post-332","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-mol-bio","8":"tag-digital-pcr","9":"entry"},"_selected_authors":[],"_selected_reviewers":[],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.8 (Yoast SEO v27.8) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>\u6b21\u4e16\u4ee3\u306e\u5b9a\u91cf\u6280\u8853\uff01\u30c7\u30b8\u30bf\u30ebPCR\u306e\u539f\u7406\u3068\u306f\uff1f - Learning at the Bench<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/digitalpcr1\/\" \/>\n<meta property=\"og:locale\" content=\"ja_JP\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"\u6b21\u4e16\u4ee3\u306e\u5b9a\u91cf\u6280\u8853\uff01\u30c7\u30b8\u30bf\u30ebPCR\u306e\u539f\u7406\u3068\u306f\uff1f\" \/>\n<meta property=\"og:description\" content=\"\u6b21\u4e16\u4ee3\u306e\u5b9a\u91cf\u6280\u8853\u3068\u3057\u3066\u30016\u56de\u306e\u30b7\u30ea\u30fc\u30ba\u3067\u30c7\u30b8\u30bf\u30ebPCR\u306e\u6280\u8853\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002\u307e\u305a\u3001\u30c7\u30b8\u30bf\u30ebPCR (dPCR) \u306b\u3064\u3044\u3066\u6982\u8981\u3092\u3054\u7d39\u4ecb\u3057\u3001\u30c7\u30b8\u30bf\u30ebPCR\u306e\u3069\u3053\u304c\u6b21\u4e16\u4ee3\u306e\u6280\u8853\u306a\u306e\u304b\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR (qPCR)\u306a\u3069\u306e\u5f93\u6765\u306ePCR\u3068\u4f55\u304c\u9055\u3046\u306e\u304b\u306b\u3064\u3044\u3066\u3054\u8aac\u660e\u3057\u307e\u3059\u3002\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/digitalpcr1\/\" \/>\n<meta property=\"og:site_name\" content=\"Learning at the Bench\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/ThermoFisherJapan\" \/>\n<meta property=\"article:published_time\" 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