{"id":338,"date":"2020-04-01T00:00:00","date_gmt":"2020-03-31T15:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/immunohistochemistry1\/"},"modified":"2025-08-12T17:35:41","modified_gmt":"2025-08-12T08:35:41","slug":"immunohistochemistry1","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/immunohistochemistry1\/","title":{"rendered":"\u306f\u3058\u3081\u3066\u306e\u514d\u75ab\u7d44\u7e54\u5316\u5b66(Immunohistochemistry; IHC)\u307e\u3068\u3081 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9\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u56fa\u5b9a\u6cd5\u306e\u6c7a\u5b9a\u306b\u306f\u3001\u88681\uff08\u6587\u732e2-5\uff09\u306e\u3088\u3046\u306a\u30ac\u30a4\u30c9\u30e9\u30a4\u30f3\u3082\u6709\u7528\u3067\u3059\u304c\u3001\u3042\u308b\u56fa\u5b9a\u6cd5\u3067\u540c\u4e00\u6297\u539f\u4e0a\u306e\u30a8\u30d4\u30c8\u30fc\u30d7\u304c\u7dad\u6301\u3055\u308c\u3066\u3044\u305f\u3068\u3057\u3066\u3082\u3001\u4ed6\u306e\u30a8\u30d4\u30c8\u30fc\u30d7\u306f\u7834\u58ca\u3055\u308c\u3066\u3044\u308b\u3053\u3068\u3082\u3042\u308a\u307e\u3059\u3002<strong>\u56fa\u5b9a\u6cd5\u306e\u9078\u629e\u306b\u306f\u7d30\u5fc3\u306e\u6ce8\u610f\u304c\u5fc5\u8981\u3067\u3042\u308a\u3001\u56fa\u5b9a\u6db2\u306e\u7a2e\u985e\u3068\u6fc3\u5ea6\u3001\u56fa\u5b9a\u6642\u9593\u3001\u6e29\u5ea6\u3001\u56fa\u5b9a\u5f8c\u51e6\u7406\u3092\u8003\u616e\u3059\u308b\u5fc5\u8981<\/strong>\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<h2>\u4e3b\u306a\u56fa\u5b9a\u6db2<\/h2>\n<h3>\u30db\u30eb\u30e0\u30a2\u30eb\u30c7\u30d2\u30c9<\/h3>\n<p>\u30db\u30eb\u30e0\u30a2\u30eb\u30c7\u30d2\u30c9\u306f\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u4e3b\u306b\u5869\u57fa\u6027\u30a2\u30df\u30ce\u9178\u3068\u53cd\u5fdc\u3057\u3066\u30e1\u30c1\u30ec\u30f3\u67b6\u6a4b\u3092\u5f62\u6210\u3001\u7d44\u7e54\u3092\u5b89\u5b9a\u5316\u3055\u305b\u307e\u3059\u3002\u30e1\u30c1\u30ec\u30f3\u67b6\u6a4b\u306f\u30db\u30eb\u30e0\u30a2\u30eb\u30c7\u30d2\u30c9\u6fc3\u5ea6\u3001\u6e29\u5ea6\u3001pH\u3001\u6642\u9593\u306b\u4f9d\u5b58\u3057\u307e\u3059\u3002<strong>\u30db\u30eb\u30e0\u30a2\u30eb\u30c7\u30d2\u30c9\u306b\u3088\u308b\u56fa\u5b9a\u306f\u6bd4\u8f03\u7684\u304a\u3060\u3084\u304b\u3067\u3001(\u5b8c\u5168\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u304c)\u53ef\u9006\u7684\u3067\u3001\u5206\u5b50\u30b5\u30a4\u30ba\u3082\u5c0f\u3055\u3044\u305f\u3081\u7d44\u7e54\u3078\u306e\u6d78\u900f\u3082\u826f\u597d\u3067\u3059\u3002\u6b20\u70b9\u3068\u3057\u3066\u306f\u56fa\u5b9a\u3057\u305f\u7d44\u7e54\u304c\u7e2e\u3093\u3060\u308a\u3001\u6b6a\u3080\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002<\/strong>\u307e\u305f\u3001\u30db\u30eb\u30e0\u30a2\u30eb\u30c7\u30d2\u30c9\u306f\u7a7a\u6c17\u4e2d\u306e\u9178\u7d20\u306b\u3088\u3063\u3066\u9178\u5316\u3055\u308c\u3066\u30ae\u9178\u3092\u751f\u3058\u3001\u6700\u7d42\u7684\u306b\u30d1\u30e9 \u30db\u30eb\u30e0\u30a2\u30eb\u30c7\u30d2\u30c9\u306b\u518d\u91cd\u5408\u3059\u308b\u305f\u3081\u3001\u4f7f\u7528\u76f4\u524d\u306b\u30d1\u30e9\u30db\u30eb\u30e0\u30a2\u30eb\u30c7\u30d2\u30c9\u304b\u3089\u30d5\u30ec\u30c3\u30b7\u30e5\u306a\u30db\u30eb\u30e0\u30a2\u30eb\u30c7\u30d2\u30c9\u6eb6\u6db2\u3092\u8abf\u88fd\u3059\u308b\u304b\u3001\u5b89\u5b9a\u3055\u305b\u308b\u305f\u308110% \u30e1\u30bf\u30ce\u30fc\u30eb\u3092\u6dfb\u52a0\u3057\u307e\u3059\u3002\u5f0a\u793e\u3067\u306f\u3001\u4e0d\u6d3b\u6027\u30ac\u30b9\u3092\u5145\u586b\u3057\u305f\u30a2\u30f3\u30d7\u30eb\u5c01\u5165\uff08\u30e1\u30bf\u30ce\u30fc\u30eb\u4e0d\u542b\uff09\u306e<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/28906?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa48_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">16% Formaldehyde (w\/v), Methanol-free<\/a>\u3092\u30e9\u30f3\u30ca\u30c3\u30d7\u3057\u3066\u3044\u307e\u3059\u3002\u30db\u30eb\u30e0\u30a2\u30eb\u30c7\u30d2\u30c9\u3092\u542b\u3080\u56fa\u5b9a\u6db2\u3068\u3057\u3066\u306f\u30014% \u30d1\u30e9\u30db\u30eb\u30e0\u30a2\u30eb\u30c7\u30d2\u30c9\u3001\u30d6\u30a2\u30f3\u56fa\u5b9a\u6db2\uff08\u98fd\u548c\u30d4\u30af\u30ea\u30f3\u9178\uff0b\u30db\u30eb\u30de\u30ea\u30f3\uff0b\u6c37\u9162\u9178\uff09\u306810% \u30ea\u30f3\u9178\u7de9\u885d\u30db\u30eb\u30de\u30ea\u30f3\u306a\u3069\u304c\u4e00\u822c\u7684\u3067\u3059\u3002<\/p>\n<h3>\u30b0\u30eb\u30bf\u30eb\u30a2\u30eb\u30c7\u30d2\u30c9<\/h3>\n<p>\u30b0\u30eb\u30bf\u30eb\u30a2\u30eb\u30c7\u30d2\u30c9\u306f\u30a2\u30df\u30ce\u57fa\u3001\u30b9\u30eb\u30d5\u30d2\u30c9\u30ea\u30eb\u57fa(\u304a\u3088\u3073\u82b3\u9999\u74b0)\u3068\u53cd\u5fdc\u3057\u307e\u3059\u3002<strong>\u30db\u30eb\u30e0\u30a2\u30eb\u30c7\u30d2\u30c9\u3088\u308a\u3082\u5f37\u529b\u306a\u30bf\u30f3\u30d1\u30af\u67b6\u6a4b\u5264\u3067\u3059\u304c\u3001\u7d44\u7e54\u3078\u306e\u6d78\u900f\u306f\u30db\u30eb\u30e0\u30a2\u30eb\u30c7\u30d2\u30c9\u3088\u308a\u3082\u9045\u304f\u3001\u6c34\u6eb6\u6027\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u69cb\u9020\u304c\u58ca\u308c\u308b\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u3002<\/strong>\u30b0\u30eb\u30bf\u30eb\u30a2\u30eb\u30c7\u30d2\u30c9\u3092\u7528\u3044\u3066\u56fa\u5b9a\u3055\u308c\u305f\u7d44\u7e54\u306f\u691c\u51fa\u524d\u306b\u30a2\u30df\u30f3\u3092\u542b\u6709\u3059\u308b\u30d0\u30c3\u30d5\u30a1\u30fc\u3067\u524d\u51e6\u7406\u3092\u304a\u3053\u306a\u3044\u3001(\u6297\u4f53\u306e\u30a2\u30df\u30f3\u3068\u5171\u6709\u7d50\u5408\u3059\u308b\u53ef\u80fd\u6027\u306e\u3042\u308b)\u672a\u53cd\u5fdc\u306e\u30a2\u30eb\u30c7\u30d2\u30c9\u57fa\u3092\u30d6\u30ed\u30c3\u30af\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u306b\u306f\u30a8\u30bf\u30ce\u30fc\u30eb\u30a2\u30df\u30f3\u3084\u30ea\u30b7\u30f3\u304c\u7528\u3044\u3089\u308c\u307e\u3059\uff08\u6587\u732e1\uff09\u30024% \u30db\u30eb\u30e0\u30a2\u30eb\u30c7\u30d2\u30c9\u30680.1% \u30b0\u30eb\u30bf\u30eb\u30a2\u30eb\u30c7\u30d2\u30c9\u306e0.1M \u30ea\u30f3\u9178\u7de9\u885d\u6eb6\u6db2\u30015% \u30b0\u30eb\u30bf\u30eb\u30a2\u30eb\u30c7\u30d2\u30c9\u306e0.1M \u30ab\u30b3\u30b8\u30eb\u9178\/1% \u30d4\u30ed\u4e9c\u786b\u9178\u30ca\u30c8\u30ea\u30a6\u30e0\u6eb6\u6db2, pH7.5-7.8\u304c\u4e00\u822c\u7684\u3067\u3059\u3002<\/p>\n<h3>DMS<\/h3>\n<p>\u30b8\u30a4\u30df\u30c9\u30a8\u30b9\u30c6\u30eb\u3067\u3042\u308b<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/20700?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa48_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Dimethyl Suberimidate (DMS)<\/a>\u3082\u67b6\u6a4b\u5264\u3068\u3057\u3066\u30b0\u30eb\u30bf\u30eb\u30a2\u30eb\u30c7\u30d2\u30c9\u306e\u4ee3\u308f\u308a\u306b\u7d44\u7e54\u306e\u56fa\u5b9a\u3067\u4f7f\u7528\u3055\u308c\u307e\u3059\uff08\u6587\u732e6\uff09\u3002DMS\u306f\u30bf\u30f3\u30d1\u30af\u8cea\u306eN\u672b\u7aef\u30a2\u30df\u30f3\u304a\u3088\u3073\u30ea\u30b8\u30f3\u5074\u9396\u30a2\u30df\u30f3\u3092\u67b6\u6a4b\u3059\u308b\u4e8c\u4fa1\u306e\u30db\u30e2\u67b6\u6a4b\u5264\u3067\u3059\u3002<strong>DMS\u306f\u5149\u5b66\u9855\u5fae\u93e1\u3084\u96fb\u5b50\u9855\u5fae\u93e1\u306e\u56fa\u5b9a\u306b\u4f7f\u7528\u3055\u308c\u3001\u6297\u539f\u6027\u304c\u7dad\u6301\u3055\u308c\u3084\u3059\u3044\u3053\u3068\u3001\u30a2\u30eb\u30c7\u30d2\u30c9\u57fa\u306e\u30d6\u30ed\u30c3\u30af\u304c\u5fc5\u8981\u306a\u3044\u3053\u3068\u304c\u5229\u70b9\u3068\u3057\u3066\u6319\u3052\u3089\u308c\u307e\u3059\u3002<\/strong><\/p>\n<h3>\u30a2\u30eb\u30b3\u30fc\u30eb<\/h3>\n<p>\u30a8\u30bf\u30ce\u30fc\u30eb\u3001\u30e1\u30bf\u30ce\u30fc\u30eb\u3001\u30a2\u30bb\u30c8\u30f3\u306a\u3069\u306e\u30bf\u30f3\u30d1\u30af\u6c88\u6bbf\u5264\u3092\u7528\u3044\u308b\u6790\u51fa\u6cd5\u306f\u3001<strong>\u9ad8\u5206\u5b50\u91cf\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3082\u6c88\u6bbf\u3055\u305b\u3001\u7d30\u80de\u8a66\u6599\u306e\u4fdd\u5b58\u306b\u6709\u52b9<\/strong>\u3067\u3059\u3002\u305f\u3060\u3057\u3001\u3053\u308c\u3089\u306e\u6c88\u6bbf\u5264\u306f<strong>\u7d44\u7e54\u3078\u306e\u900f\u904e\u6027\u304c\u4f4e\u304f\u3001\u56fa\u5b9a\u304c\u4e0d\u5b8c\u5168\u306b\u306a\u308b\u3053\u3068\u3082\u3042\u308a\u3001\u307e\u305f\u7d44\u7e54\u306e\u53ce\u7e2e\u304c\u751f\u3058\u308b\u305f\u3081\u96fb\u5b50\u9855\u5fae\u93e1\u306e\u56fa\u5b9a\u306b\u306f\u4e0d\u5411\u304d<\/strong>\u3067\u3059\u3002<\/p>\n<table>\n<caption>\n<p class=\"al-c\">\u88681 \u56fa\u5b9a\u6cd5\u4e00\u89a7<\/p>\n<\/caption>\n<tbody>\n<tr>\n<td>\n<p class=\"al-c\"><strong>\u6297\u539f<\/strong><\/p>\n<\/td>\n<td>\n<p class=\"al-c\"><strong>\u63a8\u5968\u3055\u308c\u308b\u56fa\u5b9a\u6cd5<\/strong><\/p>\n<\/td>\n<\/tr>\n<tr>\n<td>\u4f4e\u5206\u5b50\u91cf\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3001\u30da\u30d7\u30c1\u30c9\u3001\u9175\u7d20<\/td>\n<td>\u30db\u30eb\u30e0\u30a2\u30eb\u30c7\u30d2\u30c9\u3001\u30d1\u30e9\u30db\u30eb\u30e0\u30a2\u30eb\u30c7\u30d2\u30c9\u3001\u30d1\u30e9\u30db\u30eb\u30e0\u30a2\u30eb\u30c7\u30d2\u30c9-\u30d4\u30af\u30ea\u30f3\u9178<\/td>\n<\/tr>\n<tr>\n<td>\u9175\u7d20\u3068\u30da\u30d7\u30c1\u30c9<\/td>\n<td>\u30d1\u30e9\u30db\u30eb\u30e0\u30a2\u30eb\u30c7\u30d2\u30c9-\u30d4\u30af\u30ea\u30f3\u9178\u3001\u30b0\u30eb\u30bf\u30eb\u30a2\u30eb\u30c7\u30d2\u30c9<\/td>\n<\/tr>\n<tr>\n<td>\u30a2\u30df\u30ce\u9178\u306a\u3069\u306e\u4f4e\u5206\u5b50<\/td>\n<td>\u30b0\u30eb\u30bf\u30eb\u30a2\u30eb\u30c7\u30d2\u30c9<\/td>\n<\/tr>\n<tr>\n<td>\u7d30\u80de\u8cea\u5185\u6297\u539f\uff1b\u30ea\u30f3\u30d1\u7bc0\u306e\u751f\u4f53\u7d44\u7e54\u691c\u67fb<\/td>\n<td>\u5869\u5316\u6c34\u9280<\/td>\n<\/tr>\n<tr>\n<td>\u819c\u30bf\u30f3\u30d1\u30af\u8cea<\/td>\n<td>\u9162\u9178-\u5869\u5316\u4e9c\u925b<\/td>\n<\/tr>\n<tr>\n<td>\u7cd6\u30bf\u30f3\u30d1\u30af\u8cea<\/td>\n<td>\u904e\u30e8\u30a6\u7d20\u9178-\u30ea\u30b7\u30f3-\u30d1\u30e9\u30db\u30eb\u30e0\u30a2\u30eb\u30c7\u30d2\u30c9<\/td>\n<\/tr>\n<tr>\n<td>\u9ad8\u5206\u5b50\u91cf\u30bf\u30f3\u30d1\u30af\u8cea\u6297\u539f(\u30a4\u30e0\u30ce\u30b0\u30ed\u30d6\u30ea\u30f3)<\/td>\n<td>\u6c88\u6fb1\u5264(\u30a8\u30bf\u30ce\u30fc\u30eb\u3001\u30e1\u30bf\u30ce\u30fc\u30eb\u3001\u30a2\u30bb\u30c8\u30f3)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h2>\u5207\u7247\u306e\u4f5c\u6210<\/h2>\n<p><strong>\u56fa\u5b9a\u3057\u305f\u7d44\u7e54\u304b\u3089\u306e\u5207\u7247\u4f5c\u6210\u306b\u306f\u3001\u51cd\u7d50\u5207\u7247\u3068\u5305\u57cb\u5207\u7247\u304c\u3042\u308a\u307e\u3059\u3002\u51cd\u7d50\u5207\u7247\u306f\u71b1\u3084\u6709\u6a5f\u6eb6\u5a92\u306b\u3088\u308b\u5909\u6027\u3092\u3046\u3051\u306a\u3044\u305f\u3081\u3001\u6297\u539f\u6027\u304c\u7dad\u6301\u3055\u308c\u3084\u3059\u3044\u3068\u3044\u3046\u5229\u70b9\u304c\u3042\u308a\u307e\u3059\u304c\u3001\u7d44\u7e54\u5f62\u614b\u306e\u7dad\u6301\u304c\u96e3\u3057\u3044\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002<\/strong><\/p>\n<p>\u51cd\u7d50\u5207\u7247\u306e\u4f5c\u6210\u306f\u30b7\u30e7\u7cd6\u6fc3\u5ea6\u3092\u4e0a\u3052\u306a\u304c\u3089\u7d44\u7e54\u3092\u30b7\u30e7\u7cd6\u6eb6\u6db2\u306b\u7f6e\u63db\u3001\u7d9a\u3044\u3066\u6025\u51b7\u3059\u308b\u3053\u3068\u3067\u307e\u305a\u51cd\u7d50\u30d6\u30ed\u30c3\u30af\u3092\u4f5c\u6210\u3057\u3001\u51cd\u7d50\u30d6\u30ed\u30c3\u30af\u306f\u30af\u30e9\u30a4\u30aa\u30b9\u30bf\u30c3\u30c8\u306b\u3088\u308a\u5207\u7247\u3092\u4f5c\u6210\u3057\u307e\u3059\u3002<\/p>\n<p><strong>\u5305\u57cb\u5207\u7247\u306e\u4f5c\u6210\u306f\u30d1\u30e9\u30d5\u30a3\u30f3\u5305\u57cb\u304c\u6700\u3082\u4e00\u822c\u7684<\/strong>\u3067\u3059\u3002\u7d44\u7e54\u306e\u8131\u6c34\u3068\u8131\u8102\u3092\u884c\u3046\u305f\u3081\u3001\u307e\u305a\u6fc3\u5ea6\u3092\u4e0a\u6607\u3055\u305b\u306a\u304c\u3089\u30a8\u30bf\u30ce\u30fc\u30eb\u306b\u7f6e\u63db\u3001\u3064\u3065\u3044\u3066\u30ad\u30b7\u30ec\u30f3\u306a\u3069\u306e\u6709\u6a5f\u6eb6\u5a92(\u4e2d\u9593\u6eb6\u5a92)\u306b\u7f6e\u63db\u3057\u307e\u3059\u3002\u878d\u89e3\u3055\u305b\u305f\u30d1\u30e9\u30d5\u30a3\u30f3\u5185\u3067\u30d1\u30e9\u30d5\u30a3\u30f3\u6d74\u3092\u304a\u3053\u306a\u3044\u3001\u7d44\u7e54\u306b\u6d78\u900f\u3055\u305b\u305f\u3042\u3068\u51b7\u5374\u3057\u3066\u30d1\u30e9\u30d5\u30a3\u30f3\u30d6\u30ed\u30c3\u30af\u3092\u4f5c\u6210\u3057\u307e\u3059\u3002\u30df\u30af\u30ed\u30c8\u30fc\u30e0\u3067\u4f5c\u6210\u3057\u305f\u5207\u7247\uff08\u6570\u03bcm\uff09\u3092\u30b9\u30e9\u30a4\u30c9\u30ac\u30e9\u30b9\u306b\u56fa\u5b9a\u3057\u305f\u3042\u3068\u3001\u8131\u6c34\u30fb\u8131\u8102\u3068\u306f\u9006\u306e\u624b\u9806\u3067\u30a8\u30bf\u30ce\u30fc\u30eb\u30fb\u7d14\u6c34\u3078\u306e\u7f6e\u63db\u3092\u9806\u6b21\u304a\u3053\u306a\u3044\u8131\u30d1\u30e9\u30d5\u30a3\u30f3\u30fb\u89aa\u6c34\u5316\u3057\u307e\u3059\u3002<\/p>\n<h2>\u5185\u5728\u6027\u7269\u8cea\u306b\u3088\u308b\u963b\u5bb3<\/h2>\n<p><strong>\u7d44\u7e54\u306b\u306f\u514d\u75ab\u7d44\u7e54\u5316\u5b66\u3067\u4f7f\u7528\u3055\u308c\u308b\u8a66\u85ac\u3068\u53cd\u5fdc\u3059\u308b\u9175\u7d20\u3001\u30d3\u30bf\u30df\u30f3\u3001\u30d3\u30aa\u30c1\u30f3\u306a\u3069\u306e\u7269\u8cea\u304c\u5185\u5728\u3057\u3066\u3044\u307e\u3059\u3002<\/strong>\u7d30\u80de\u3084\u7d44\u7e54\u306e\u514d\u75ab\u67d3\u8272\u3067\u4e00\u822c\u7684\u306b\u7528\u3044\u3089\u308c\u308b\u514d\u75ab\u30d1\u30fc\u30aa\u30ad\u30b7\u30c0\u30fc\u30bc\u6cd5\u3067\u306f\u3001\u7d44\u7e54\u306b\u5185\u5728\u3059\u308b\u30d1\u30fc\u30aa\u30ad\u30b7\u30c0\u30fc\u30bc(\u30d7\u30bd\u30a4\u30c9\u30da\u30eb\u30aa\u30ad\u30b7\u30c0\u30fc\u30bc)\u304c\u904e\u9178\u5316\u6c34\u7d20\u3068\u53cd\u5fdc\u3057\u3066DAB\u306a\u3069\u306e\u30d1\u30fc\u30aa\u30ad\u30b7\u30c0\u30fc\u30bc\u57fa\u8cea\u3092\u767a\u8272\u3055\u305b\u308b\u305f\u3081\u3001\u6297\u539f\u6297\u4f53\u53cd\u5fdc\u306b\u3088\u3063\u3066\u6297\u4f53\u5206\u5b50\u304c\u5b58\u5728\u3059\u308b\u5834\u6240\u3068\u306f\u95a2\u4fc2\u306a\u304f\u3001\u5185\u5728\u6027\u30d1\u30fc\u30aa\u30ad\u30b7\u30c0\u30fc\u30bc\u306e\u5c40\u5728\u3059\u308b\u5834\u6240\u3067\u30b7\u30b0\u30ca\u30eb\u304c\u751f\u3058\u307e\u3059\u3002\u3053\u306e\u3088\u3046\u306a\u5185\u5728\u6027\u30d1\u30fc\u30aa\u30ad\u30b7\u30c0\u30fc\u30bc\u306f\u3001\u56fa\u5b9a\u3057\u305f\u7d44\u7e54\u3092\u6297\u4f53\u7121\u3057\u306e\u6761\u4ef6\u3067\uff08\u4ed6\u306e\u5b9f\u9a13\u64cd\u4f5c\u306f\u672c\u5b9f\u9a13\u3068\u5168\u304f\u540c\u4e00\u306b\uff09\u3001DAB\u767a\u8272\u3059\u308b\u3053\u3068\u3067\u78ba\u8a8d\u3067\u304d\u307e\u3059\u3002\u305d\u3057\u3066\u3001\u5185\u5728\u3059\u308b\u5834\u5408\u306b\u306f\u3001\u6a19\u8b58\u306b\u7528\u3044\u308b\u9175\u7d20\u3092\u5909\u66f4\u3059\u308b\u304b\u3001\u5185\u5728\u6027\u30d1\u30fc\u30aa\u30ad\u30b7\u30c0\u30fc\u30bc\u3092\u4e0d\u6d3b\u6027\u5316\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u4e0d\u6d3b\u6027\u5316\u3067\u306f\u30013% \u904e\u9178\u5316\u6c34\u7d20\u6c34\u6eb6\u6db2\uff08\u6587\u732e7\uff09\u307e\u305f\u306f\u904e\u9178\u5316\u6c34\u7d20\u30e1\u30bf\u30ce\u30fc\u30eb\u6eb6\u6db2\uff08\u6587\u732e8\uff09\u306b\u3088\u308a\u51e6\u7406\u3057\u307e\u3059\u3002<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/35000?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa48_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Peroxidase Suppresor<\/a>\u3067\u306f\u3001\u904e\u9178\u5316\u6c34\u7d20\u6c34\u6eb6\u6db2\u307e\u305f\u306f\u30e1\u30bf\u30ce\u30fc\u30eb\u6eb6\u6db2\u306b\u6bd4\u3079\u3066\u3001\u3088\u308a\u52b9\u679c\u7684\u306b\u5185\u5728\u3059\u308b\u30d1\u30fc\u30aa\u30ad\u30b7\u30c0\u30fc\u30bc\u6d3b\u6027\u3092\u963b\u5bb3\u3067\u304d\u307e\u3059\uff08\u6587\u732e9\uff09\u3002<\/p>\n<p><strong>\u30a2\u30d3\u30b8\u30f3\u3092\u7528\u3044\u3066\u6297\u539f\u306e\u691c\u51fa\u3092\u5897\u5e45\u3059\u308bABC\u67d3\u8272\u3067\u306f\u3001\u809d\u81d3\u3001\u4e73\u817a\u3001\u8102\u80aa\u7d44\u7e54\u3001\u814e\u81d3\u306a\u3069\u306e\u5185\u56e0\u6027\u30d3\u30aa\u30c1\u30f3\u306b\u5bcc\u3080\u7d44\u7e54\u3067\u306f\u3001\u975e\u7279\u7570\u7684\u30b7\u30b0\u30ca\u30eb\u304c\u751f\u3058\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059<\/strong>\uff08\u6587\u732e10\uff09\u3002\u3053\u306e\u5185\u5728\u6027\u30d3\u30aa\u30c1\u30f3\u306e\u6d3b\u6027\u306f\u7279\u306b\u51cd\u7d50\u5207\u7247\u3067\u9855\u8457\u306b\u307f\u3089\u308c\u3001\u30a2\u30d3\u30b8\u30f3\u306e\u6dfb\u52a0\u3001\u304a\u3088\u3073\u6dfb\u52a0\u3057\u305f\u30a2\u30d3\u30b8\u30f3\u306e\u7d50\u5408\u30b5\u30a4\u30c8\u3092\u30d6\u30ed\u30c3\u30af\u3059\u308b\u305f\u3081\u306b\u30d3\u30aa\u30c1\u30f3\u3092\u6dfb\u52a0\u3059\u308b\u3053\u3068\u3067\u5185\u5728\u6027\u30d3\u30aa\u30c1\u30f3\u3092\u6291\u5236\u3057\u307e\u3059\uff08\u6587\u732e12\uff09\u3002\u307e\u305f\u3001<strong>\u30a2\u30d3\u30b8\u30f3\u306f\u7cd6\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u3042\u308a\u3001\u7d44\u7e54\u306b\u5185\u5728\u3059\u308b\u30ec\u30af\u30c1\u30f3\u69d8\u5316\u5408\u7269\u3068\u7d50\u5408\u3059\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002<\/strong>\u30a2\u30d3\u30b8\u30f3\u5206\u5b50\u4e0a\u306e\u7cd6\u9396\u306b\u985e\u4f3c\u3057\u305f\u5316\u5408\u7269\u3092\u6dfb\u52a0\u3059\u308b\u3053\u3068\u3067(\u6587\u732e11)\u3001\u3053\u306e\u3088\u3046\u306a\u975e\u7279\u7570\u7684\u306a\u7d50\u5408\u3092\u6291\u3048\u308b\u3053\u3068\u3082\u3067\u304d\u307e\u3059\u304c\u3001\u7cd6\u9396\u3092\u542b\u307e\u306a\u3044<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/434301?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa48_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Streptavidin<\/a>\u3084Neutravidin\u306b\u4ee3\u66ff\u3059\u308b\u307b\u3046\u304c\u4e00\u822c\u7684\u3068\u3044\u3048\u307e\u3059\u3002<\/p>\n<h2>\u6297\u4f53\u306e\u975e\u7279\u7570\u7684\u7d50\u5408\u306e\u6291\u5236\uff08\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\uff09<\/h2>\n<p>\u7d44\u7e54\u30b5\u30f3\u30d7\u30eb\u306b\u5b58\u5728\u3059\u308b\u975e\u7279\u7570\u7684\u30b5\u30a4\u30c8\u306e\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u3082\u91cd\u8981\u3067\u3059\u3002<strong>\u4e00\u822c\u7684\u306a\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u5264\u306f\u901a\u5e38\u8840\u6e05\u3067\u3042\u308a\u3001\u4e8c\u6b21\u6297\u4f53\u3068\u540c\u7a2e\u306e\u30db\u30b9\u30c8\u304b\u3089\u5f97\u3089\u308c\u305f\u901a\u5e38\u8840\u6e05\u3092\u64cd\u4f5c\u306e\u6700\u521d\u306b\u6dfb\u52a0\u3057\u3066\u975e\u7279\u7570\u7684\u5438\u7740\u304a\u3088\u3073\uff08\u597d\u307e\u3057\u304f\u306a\u3044\uff09\u7279\u7570\u7684\u5438\u7740\u3092\u963b\u6b62\u3057\u307e\u3059\u3002<\/strong>\u76f4\u63a5\u6cd5\u3092\u7528\u3044\u308b\u5834\u5408\u3001\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u8840\u6e05\u3068\u3057\u3066\u306f\u4e00\u6b21\u6297\u4f53\u3068\u540c\u7a2e\u306e\u901a\u5e38\u8840\u6e05\u3092\u4f7f\u7528\u3057\u307e\u3059\u3002\u5f0a\u793e\u88fd\u54c1\u306e\u901a\u5e38\u8840\u6e05\u306b\u306f\u3001<a href=\"https:\/\/www.thermofisher.com\/order\/genome-database\/antibody\/Normal-Goat-Serum-Control\/31872?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa48_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">\u30e4\u30ae<\/a>\u3001<a href=\"https:\/\/www.thermofisher.com\/order\/genome-database\/antibody\/Normal-Horse-Serum-Control\/31874?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa48_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">\u30a6\u30de<\/a>\u3001<a href=\"https:\/\/www.thermofisher.com\/order\/genome-database\/antibody\/Normal-Human-Serum-Control\/31876?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa48_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">\u30d2\u30c8<\/a>\u3001<a href=\"https:\/\/www.thermofisher.com\/order\/genome-database\/antibody\/Normal-Mouse-Serum-Control\/31880?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa48_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">\u30de\u30a6\u30b9<\/a>\u3001<a href=\"https:\/\/www.thermofisher.com\/order\/genome-database\/antibody\/Normal-Rabbit-Serum-Control\/31883?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa48_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">\u30a6\u30b5\u30ae<\/a>\u3001<a href=\"https:\/\/www.thermofisher.com\/order\/genome-database\/antibody\/Normal-Rat-Serum-Control\/31888?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa48_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">\u30e9\u30c3\u30c8<\/a>\u3001<a href=\"https:\/\/www.thermofisher.com\/order\/genome-database\/antibody\/Normal-Porcine-Serum-Control\/31890?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa48_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">\u30d6\u30bf<\/a>\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<p><strong>\u56fa\u5b9a\u3057\u305f\u7d44\u7e54\u30b5\u30f3\u30d7\u30eb\u306e\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u3067\u306f\u3001\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u30d0\u30c3\u30d5\u30a1\u30fc\u3068\u306e\u53cd\u5fdc\u3092\u4e00\u6b21\u6297\u4f53\u306e\u6dfb\u52a0\u524d\u306b10-30\u5206\u307b\u3069\u884c\u3044\u307e\u3059\u3002<\/strong>\u30b5\u30f3\u30d7\u30eb\u306e\u6d17\u6d44\u306b\u3088\u308a\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u5264\u304c\u306e\u52b9\u679c\u4f4e\u6e1b\u3092\u9632\u3050\u305f\u3081\u3001\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u5f8c\u306f\u3001\u6d17\u6d44\u305b\u305a\u306b\u5358\u306b\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u30d0\u30c3\u30d5\u30a1\u30fc\u3092\u9664\u304f\u3060\u3051\u3067\u3059\u3002\u4e00\u6b21\u6297\u4f53\u3068\u53cd\u5fdc\u3055\u305b\u305f\u30b5\u30f3\u30d7\u30eb\u3092\u6d17\u6d44\u3057\u305f\u5f8c\u306f\u3001\u4e8c\u6b21\u6297\u4f53\u306e\u6dfb\u52a0\u524d\u306b\u518d\u5ea6\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u3092\u884c\u3046\u3079\u304d\u3067\u3059\u304c\u3001\u4e8c\u6b21\u6297\u4f53\u304c\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u30d0\u30c3\u30d5\u30a1\u30fc\u3067\u5e0c\u91c8\u3055\u308c\u3066\u3044\u308b\u5834\u5408\u306f\u5fc5\u8981\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u758e\u6c34\u7684\u76f8\u4e92\u4f5c\u7528\u3092\u8efd\u6e1b\u3057\u3066\u30d0\u30c3\u30af\u30b0\u30e9\u30f3\u30c9\u30ce\u30a4\u30ba\u3092\u4f4e\u4e0b\u3055\u305b\u308b\u305f\u3081\u30010.05% Tween-20\u306a\u3069\u306e\u754c\u9762\u6d3b\u6027\u5264\u304c\u6709\u52b9\u306a\u5834\u5408\u3082\u3042\u308a\u307e\u3059\u3002<\/p>\n<h2>\u53c2\u8003\u6587\u732e<\/h2>\n<ol>\n<li>Coons, A.A., et al. (1942). J. Immunol. 45, 159-170.<\/li>\n<li>Culling, C.F.A. (1974). Handbook of Histopathological and Histochemical Techniques, 3rd ed. Butterworths, London.<\/li>\n<li>Lillie, R.D. and Fullmer, M. (1976). Histopathologic Technique and Practical Histochemistry, 4th ed. McGraw Hill Book Co., New York.<\/li>\n<li>Pearse, A.G.E. (1980). Histochemistry, Theoretical and Applied, 4th ed., Vol 1. Preparative and Optical Technology. Churchill Livingstone, London.<\/li>\n<li>Larsson, L.I. (1988). Immunocytochemistry: Theory and Practice. CRC Press, Boca Raton, FL.<\/li>\n<li>Hassel, J. and Hand, A.R. (1973). J. Histochem. Cytochem. 22, 229-239.<\/li>\n<li>K\u00f6ller, U., et al. (1986). J. Immunol. Methods 86, 75.<\/li>\n<li>Streefkerk, J.G. (1972). Nature 330, 80.<\/li>\n<li>Fink, B., et al. (1979). J. Histochem. Cytochem. 27, 1299.<\/li>\n<li>Dakshinamurti, K. and Mistry, S.P. (1963). J. Biol. Chem. 238, 294.<\/li>\n<li>Naritoku, W.Y. and Taylor, C.R. (1982). J. Histochem. Cytochem. 30, 253-260.<\/li>\n<li>Wood, G.S. and Warnke, R. (1981). J. Histochem. Cytochem. 29, 1196-1204.<\/li>\n<\/ol>\n<h1>\u3010\u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3011\u30bf\u30f3\u30d1\u30af\u8cea\u89e3\u6790\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u30cf\u30f3\u30c9\u30d6\u30c3\u30af<\/h1>\n<p>\u52b9\u7387\u7684\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u62bd\u51fa\u304b\u3089\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u89e3\u6790\u30c4\u30fc\u30eb\u307e\u3067\u3001\u5305\u62ec\u7684\u306b\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u3092\u7d39\u4ecb\u3057\u3066\u304a\u308a\u307e\u3059\u3002PDF\u30d5\u30a1\u30a4\u30eb\u306e\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3092\u3054\u5e0c\u671b\u306e\u65b9\u306f\u3001\u4e0b\u8a18\u30dc\u30bf\u30f3\u3088\u308a\u304a\u7533\u8fbc\u307f\u304f\u3060\u3055\u3044\u3002<\/p>\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/catalogs-protein-form.html?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa48_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/p>\n<h2>\u7d44\u7e54\u67d3\u8272\u30fb\u514d\u75ab\u7d44\u7e54\u5316\u5b66\u306e\u57fa\u790e\u306b\u95a2\u3059\u308b\u8a18\u4e8b\u306f\u3053\u3061\u3089<\/h2>\n<table style=\"border-color: lightgray;border-collapse: collapse;padding: 20px\" border=\"1\">\n<tbody>\n<tr>\n<td style=\"border-color: lightgray;padding: 20px\">\n<ul>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic17\/?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa48_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u7b2c17\u56de\u3011\u3088\u304f\u308f\u304b\u308b\u7d44\u7e54\u67d3\u8272\u306e\u57fa\u790e\u77e5\u8b58\n<p><\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic18\/?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa48_Social_LAB\" target=\"_blank\" 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