{"id":34,"date":"2020-03-04T00:00:00","date_gmt":"2020-03-03T15:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic2\/"},"modified":"2025-05-13T18:50:44","modified_gmt":"2025-05-13T09:50:44","slug":"protein-basic2","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic2\/","title":{"rendered":"\u30bf\u30f3\u30d1\u30af\u8cea\u5b9f\u9a13\u306b\u4f7f\u7528\u3059\u308b\u754c\u9762\u6d3b\u6027\u5264\u306e\u7279\u6027\u3068\u7a2e\u985e\uff5c\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\uff01\u30bf\u30f3\u30d1\u30af\u8cea\u5b9f\u9a13\u3042\u308c\u3053\u308c \u7b2c2\u56de"},"content":{"rendered":"<p>\u4eca\u56de\u306e\u30c6\u30fc\u30de\u306f\u300c\u754c\u9762\u6d3b\u6027\u5264\u306e\u7279\u6027\u3068\u7a2e\u985e\u300d\u3068\u984c\u3057\u3066\u3001\u754c\u9762\u6d3b\u6027\u5264\u306e\u6982\u8ad6\u3068\u30bf\u30f3\u30d1\u30af\u8cea\u5b9f\u9a13\u3067\u4f7f\u7528\u3059\u308b\u969b\u306b\u7559\u610f\u3059\u3079\u304d\u70b9\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\uff01<\/p>\n<h2>\u754c\u9762\u6d3b\u6027\u5264\u306e\u7279\u6027\u3068\u7a2e\u985e<\/h2>\n<p>\u754c\u9762\u6d3b\u6027\u5264\u306f\u8102\u80aa\u65cf\u3084\u82b3\u9999\u65cf\u304b\u3089\u306a\u308b\u975e\u6975\u6027\u90e8\u5206\uff08\u30c6\u30fc\u30eb\uff09\u3068\u6975\u6027\u90e8\u5206\uff08\u30d8\u30c3\u30c9\uff09\u306e\u4e21\u65b9\u3092\u6709\u3059\u308b\u4e21\u89aa\u5a92\u6027\u306e\u5206\u5b50\u3067\u3059\uff08\u56f31\uff09\u3002\u754c\u9762\u6d3b\u6027\u5264\u306f<strong>\u30d8\u30c3\u30c9\u30b0\u30eb\u30fc\u30d7\u306e\u6027\u8cea\u306b\u3088\u3063\u3066\u30a4\u30aa\u30f3\u6027\uff08\u6709\u3059\u308b\u96fb\u8377\u306b\u3088\u3063\u3066\u967d\u30a4\u30aa\u30f3\u6027\u307e\u305f\u306f\u9670\u30a4\u30aa\u30f3\u6027\u304c\u3042\u308b\uff09\u3001\u975e\u30a4\u30aa\u30f3\u6027\uff08\u96fb\u8377\u3092\u3082\u305f\u306a\u3044\uff09\u3001\u307e\u305f\u306f\u4e21\u30a4\u30aa\u30f3\u6027\uff08\u6b63\u8ca0\u4e21\u65b9\u306e\u96fb\u8377\u3092\u3082\u3064\u304c\u5206\u5b50\u5168\u4f53\u306e\u96fb\u8377\u306f\u30bc\u30ed\uff09\u306b\u5927\u304d\u304f\u5206\u985e\u3055\u308c\u307e\u3059\u3002<\/strong>\u5404\u754c\u9762\u6d3b\u6027\u5264\u306e\u6027\u8cea\u306f\u3001\u305d\u306e\u30d8\u30c3\u30c9\u30b0\u30eb\u30fc\u30d7\u3068\u30c6\u30fc\u30eb\u30b0\u30eb\u30fc\u30d7\u306e\u7279\u6027\uff08\u89aa\u6c34\u758e\u6c34\u6027\u306e\u30d0\u30e9\u30f3\u30b9, \u9396\u9577, \u304b\u3055\u306e\u9ad8\u3055\uff09\u306b\u3088\u3063\u3066\u6c7a\u307e\u308a\u307e\u3059\u3002\u754c\u9762\u6d3b\u6027\u5264\u306f\u6c34\u6eb6\u6027\u306e\u5206\u5b50\u3067\u3042\u308a\u3001\u975e\u6975\u6027\u306e\u30c6\u30fc\u30eb\u30b0\u30eb\u30fc\u30d7\u304c\u758e\u6c34\u76f8\u4e92\u4f5c\u7528\u306b\u95a2\u4e0e\u3059\u308b\u3053\u3068\u304b\u3089\u751f\u4f53\u819c\u3092\u69cb\u6210\u3059\u308b\u30ea\u30f3\u8102\u8cea\u306b\u985e\u4f3c\u3057\u305f\u6027\u8cea\u3092\u6709\u3057\u3066\u304a\u308a\u3001\u6c34\u306b\u4e0d\u6eb6\u6027\u306e\u758e\u6c34\u6027\u5206\u5b50\u3092\u6c34\u6eb6\u6db2\u4e2d\u306b\u5206\u6563\uff08\u6df7\u548c\uff09\u3055\u305b\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n<p><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/protein-basic2-fig1.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-82\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/protein-basic2-fig1.jpg\" alt=\"\u56f31\u3000\u754c\u9762\u6d3b\u6027\u5264\u5206\u5b50\u306e\u30a4\u30e1\u30fc\u30b8\u56f3\uff08\u4e0a\u56f3\uff09\u3068\u4e21\u30a4\u30aa\u30f3\u6027\u754c\u9762\u6d3b\u6027\u5264\uff08CHAPS\uff09\u306e\u69cb\u9020\u5f0f\uff08\u4e0b\u56f3\uff09\" width=\"500\" height=\"316\" \/><\/a><\/p>\n<p style=\"text-align: left\">\u56f31\u3000\u754c\u9762\u6d3b\u6027\u5264\u5206\u5b50\u306e\u30a4\u30e1\u30fc\u30b8\u56f3\uff08\u4e0a\u56f3\uff09\u3068\u4e21\u30a4\u30aa\u30f3\u6027\u754c\u9762\u6d3b\u6027\u5264\uff08CHAPS\uff09\u306e\u69cb\u9020\u5f0f\uff08\u4e0b\u56f3\uff09<\/p>\n<p>&nbsp;<\/p>\n<p>\u754c\u9762\u6d3b\u6027\u5264\u306f\u6c34\u6eb6\u6027\u306e\u5206\u5b50\u3067\u3059\u304c\u3001\u6c34\u6eb6\u6db2\u4e2d\u3067\u306e\u6fc3\u5ea6\u304c\u4f4e\u3044\u3068\u304d\u306f\u901a\u5e38\u30c6\u30fc\u30eb\u30b0\u30eb\u30fc\u30d7\uff08\u758e\u6c34\u57fa\uff09\u3092\u6c17\u76f8\u5074\u306b\u5411\u3051\u305f\u72b6\u614b\u3067\u3001\u305d\u306e\u5927\u90e8\u5206\u304c\u6c17\u6db2\u754c\u9762\u306b\u96c6\u307e\u308a\u307e\u3059\u3002\u6c34\u6eb6\u6db2\u4e2d\u3067\u306e\u6fc3\u5ea6\u304c\u9ad8\u304f\u306a\u308b\u3068\u3001\u71b1\u529b\u5b66\u7684\u306b\u3088\u308a\u5b89\u5b9a\u5316\u3059\u308b\u305f\u3081\u306b\u4e92\u3044\u306b\u4f1a\u5408\u3057\u3001\u5916\u5074\u306b\u30d8\u30c3\u30c9\u30b0\u30eb\u30fc\u30d7\uff08\u89aa\u6c34\u57fa\uff09\u3001\u5185\u5074\u306b\u30c6\u30fc\u30eb\u30b0\u30eb\u30fc\u30d7\uff08\u758e\u6c34\u57fa\uff09\u3092\u3082\u3064\u30df\u30bb\u30eb\u3092\u5f62\u6210\u3057\u307e\u3059\uff08\u56f32\uff09\u3002<strong>\u754c\u9762\u6d3b\u6027\u5264\u304c\u3053\u306e\u30df\u30bb\u30eb\u3092\u5f62\u6210\u3059\u308b\u6700\u4f4e\u6fc3\u5ea6\u3092\u81e8\u754c\u30df\u30bb\u30eb\u6fc3\u5ea6\uff08Critical Micelle Concentration: CMC\uff09\u3068\u547c\u3073\u307e\u3059\u3002<\/strong>\u307e\u305f\u754c\u9762\u6d3b\u6027\u5264\u304c\u30df\u30bb\u30eb\u3092\u5f62\u6210\u3059\u308b\u3068\u304d\u306e\u6e29\u5ea6\u3092\u81e8\u754c\u30df\u30bb\u30eb\u6e29\u5ea6\uff08Critical Micelle Temperature: CMT\uff09\u3068\u547c\u3073\u307e\u3059\u3002\u754c\u9762\u6d3b\u6027\u5264\u306f\uff08CMT\u4ee5\u4e0a\u306e\u6e29\u5ea6\u3067\uff09CMC\u3088\u308a\u6fc3\u5ea6\u304c\u9ad8\u304f\u306a\u308b\u3068\u30df\u30bb\u30eb\u3092\u5f62\u6210\u3057\u305f\u900f\u660e\u306a\u6db2\u4f53\u3068\u306a\u308a\u307e\u3059\u3002CMT\u3088\u308a\u4f4e\u3044\u6e29\u5ea6\u3067\u306f\u4e0d\u6eb6\u6027\u306e\u7d50\u6676\u72b6\u614b\uff08\u307e\u305f\u306f\u30e2\u30ce\u30de\u30fc\u3068\u306e\u5171\u5b58\u72b6\u614b\uff09\u3067\u5b58\u5728\u3057\u307e\u3059\u3002\u305f\u3060\u3057CMT\u3088\u308a\u9ad8\u3044\u6e29\u5ea6\u3067\u3082\u66c7\u308a\u70b9\uff08Cloud Point\uff09\u3068\u547c\u3070\u308c\u308b\u6e29\u5ea6\u3092\u8d85\u3048\u308b\u3068\u4e0d\u900f\u660e\u306a\u6fc1\u3063\u305f\u6eb6\u6db2\u3068\u306a\u308a\u3001\u9060\u5fc3\u3059\u308b\u3068\u754c\u9762\u6d3b\u6027\u5264\u3092\u542b\u3080\u76f8\u3068\u6c34\u306e\u76f8\u3068\u306b\u5206\u96e2\uff08\u5206\u914d\uff09\u3057\u307e\u3059\u3002\u5f0a\u793e\u88fd\u54c1\u306e<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/89842?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa13_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Mem-PER\u2122 Plus Membrane Protein Extraction Kit<\/a>\u3067\u306f\u3001\u819c\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u62bd\u51fa\u3059\u308b\u305f\u3081\u306bTriton X-114\u306e\u3053\u306e\u5206\u914d\u539f\u7406\u3092\u5229\u7528\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<p><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/protein-basic2-fig2.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-84\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/protein-basic2-fig2.jpg\" alt=\"\u56f32\u3000\u6c34\u6eb6\u6db2\u4e2d\u306e\u754c\u9762\u6d3b\u6027\u5264\u30df\u30bb\u30eb\u306e\u30a4\u30e1\u30fc\u30b8\u56f3\" width=\"345\" height=\"347\" \/><\/a><\/p>\n<p style=\"text-align: left\">\u56f32\u3000\u6c34\u6eb6\u6db2\u4e2d\u306e\u754c\u9762\u6d3b\u6027\u5264\u30df\u30bb\u30eb\u306e\u30a4\u30e1\u30fc\u30b8\u56f3<\/p>\n<p>&nbsp;<\/p>\n<p>\u754c\u9762\u6d3b\u6027\u5264\u306e\u6027\u8cea\u306f\u5b9f\u9a13\u3067\u4f7f\u7528\u3059\u308b\u969b\u306e\u6fc3\u5ea6\u3001\u53cd\u5fdc\u6e29\u5ea6\u3001\u30d0\u30c3\u30d5\u30a1\u30fcpH\u3001\u30a4\u30aa\u30f3\u5f37\u5ea6\u306a\u3069\u306b\u3088\u3063\u3066\u7570\u306a\u308a\u307e\u3059\u3002\u975e\u30a4\u30aa\u30f3\u6027\u754c\u9762\u6d3b\u6027\u5264\u306eCMC\u306f\u6e29\u5ea6\u4e0a\u6607\u3068\u3068\u3082\u306b\u6e1b\u5c11\u3057\u3001\u30a4\u30aa\u30f3\u6027\u306e\u754c\u9762\u6d3b\u6027\u5264\u306f\u5bfe\u30a4\u30aa\u30f3\uff08\u30a4\u30aa\u30f3\u6027\u754c\u9762\u6d3b\u6027\u5264\u3068\u53cd\u5bfe\u306e\u96fb\u8377\u3092\u3082\u3064\u4f4e\u5206\u5b50\u30a4\u30aa\u30f3\uff09\u3092\u6dfb\u52a0\u3059\u308b\u3068\u30d8\u30c3\u30c9\u30b0\u30eb\u30fc\u30d7\u9593\u306e\u9759\u96fb\u53cd\u767a\u529b\u304c\u6e1b\u5c11\u3057CMC\u304c\u6e1b\u5c11\u3057\u307e\u3059\u3002\u307e\u305f\u3001\u30a6\u30ec\u30a2\u3092\u6dfb\u52a0\u3059\u308b\u3068\u6c34\u7d20\u7d50\u5408\u3057\u3066\u3044\u308b\u6c34\u5206\u5b50\u306e\u69cb\u9020\u304c\u304f\u305a\u308c\u3066CMC\u304c\u6e1b\u5c11\u3057\u307e\u3059\u3002<strong>\u4e00\u822c\u7684\u306b\u30a4\u30aa\u30f3\u5f37\u5ea6\u304c\u9ad8\u3044\u6eb6\u6db2\u4e2d\u3067\u306f\u4f4e\u3044\u6eb6\u6db2\u306b\u6bd4\u3079\u3066\u30df\u30bb\u30eb\u30b5\u30a4\u30ba\u304c\u5927\u304d\u304f\u306a\u308a\u307e\u3059\u3002<\/strong><\/p>\n<p>\u754c\u9762\u6d3b\u6027\u5264\u306e\u3046\u3061SDS \uff08Sodium Dodecyl Sulfate\uff09 \u306e\u3088\u3046\u306a\u30a2\u30cb\u30aa\u30f3\u6027\u754c\u9762\u6d3b\u6027\u5264\u3084CTAB (Cetyl Trimethyl Ammonium Bromide) \u306e\u3088\u3046\u306a\u30ab\u30c1\u30aa\u30f3\u6027\u754c\u9762\u6d3b\u6027\u5264\u306f\u5909\u6027\u4f5c\u7528\u3092\u3082\u3061\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u30fb\u30bf\u30f3\u30d1\u30af\u8cea\u9593\u306e\u76f8\u4e92\u4f5c\u7528\u3092\u7834\u58ca\u3057\u3066\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u5909\u6027\u3055\u305b\u307e\u3059\u3002\u4e00\u65b9\u3001TritonX-100\u306e\u3088\u3046\u306a\u975e\u30a4\u30aa\u30f3\u6027\u754c\u9762\u6d3b\u6027\u5264\u3084CHAPS\u306e\u3088\u3046\u306a\u4e21\u30a4\u30aa\u30f3\u6027\u754c\u9762\u6d3b\u6027\u5264\u3001\u30b3\u30fc\u30eb\u9178\u30ca\u30c8\u30ea\u30a6\u30e0\u3084\u30c7\u30aa\u30ad\u30b7\u30b3\u30fc\u30eb\u9178\u30ca\u30c8\u30ea\u30a6\u30e0\u306a\u3069\u306e\u80c6\u6c41\u9178\u5869\uff08\u30a2\u30cb\u30aa\u30f3\u6027\u754c\u9762\u6d3b\u6027\u5264\uff09\u306f\u975e\u5909\u6027\u7684\u306a\u754c\u9762\u6d3b\u6027\u5264\u3068\u3057\u3066\u77e5\u3089\u308c\u3066\u3044\u307e\u3059\u3002\u7279\u306b<strong>\u975e\u30a4\u30aa\u30f3\u6027\u307e\u305f\u306f\u4e21\u30a4\u30aa\u30f3\u6027\u306e\u754c\u9762\u6d3b\u6027\u5264\u306f\u4e00\u822c\u7684\u306b\u30a4\u30aa\u30f3\u6027\u306e\u754c\u9762\u6d3b\u6027\u5264\u3088\u308a\u3082\u30de\u30a4\u30eb\u30c9\u3067\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5909\u6027\u304c\u5c11\u306a\u3044<\/strong>\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n<h2>\u30bf\u30f3\u30d1\u30af\u8cea\u3078\u306e\u7d50\u5408\u3068\u53ef\u6eb6\u5316<\/h2>\n<p>\u5909\u6027\u4f5c\u7528\u3092\u3082\u3064\u754c\u9762\u6d3b\u6027\u5264\u3067\u3042\u308bSDS\u306f\u3001CMC\u4ee5\u4e0b\u306e\u6fc3\u5ea6\uff08\u30e2\u30ce\u30de\u30fc\u72b6\u614b\uff09\u3067\u819c\u30bf\u30f3\u30d1\u30af\u8cea\uff08\u758e\u6c34\u6027\uff09\u306b\u3082\u819c\u30bf\u30f3\u30d1\u30af\u8cea\u4ee5\u5916\u306e\u6c34\u6eb6\u6027\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u3082\u7d50\u5408\u3057\u307e\u3059\u3002SDS\u306f\u5206\u5b50\u540c\u58eb\u304c\u5354\u8abf\u7684\u306b\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u7d50\u5408\u3057\u30011\u5206\u5b50\u306eSDS\u304c\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u7d50\u5408\u3059\u308b\u3068\u5225\u306eSDS\u5206\u5b50\u306e\u7d50\u5408\u304c\u4fc3\u9032\u3055\u308c\u307e\u3059\u3002SDS\u304c\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u5341\u5206\u7d50\u5408\u3059\u308b\u3068\u30bf\u30f3\u30d1\u30af\u8cea\u306f\u5909\u6027\u3057\u3066\u9ad8\u6b21\u69cb\u9020\u304c\u7834\u58ca\u3055\u308c\u3001\u5168\u4f53\u3068\u3057\u3066\u307b\u307c\u5747\u4e00\u306b\u8ca0\u306e\u96fb\u8377\u3092\u5e2f\u3073\u305f\u5206\u5b50\u3068\u306a\u308a\u307e\u3059\u3002\u305d\u306e\u7d50\u679c\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u306f\u5206\u5b50\u91cf\u306b\u4f9d\u5b58\u3057\u305f\u76f4\u9396\u4e0a\u306e\u5206\u5b50\u3068\u306a\u308a\u307e\u3059\u3002\u4e00\u65b9\u3001\u975e\u30a4\u30aa\u30f3\u6027\u754c\u9762\u6d3b\u6027\u5264\u3067\u3042\u308bTriton X-100\u306f\u3001\u975e\u96fb\u8377\uff08\u975e\u30a4\u30aa\u30f3\u6027\uff09\u306e\u30d8\u30c3\u30c9\u30b0\u30eb\u30fc\u30d7\u3092\u6301\u3063\u3066\u304a\u308a\u4e00\u822c\u7684\u306b\u6c34\u6eb6\u6027\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u69cb\u9020\u3084\u76f8\u4e92\u4f5c\u7528\u306e\u7834\u58ca\u306b\u306f\u5411\u3044\u3066\u304a\u3089\u305a\u3001\u819c\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u758e\u6c34\u9818\u57df\u3068\u306e\u7d50\u5408\u3092\u5229\u7528\u3057\u3066\u819c\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u5206\u96e2\u3059\u308b\u305f\u3081\u306b\u5229\u7528\u3055\u308c\u307e\u3059\u3002<\/p>\n<p>\u754c\u9762\u6d3b\u6027\u5264\u306e\u30e2\u30ce\u30de\u30fc\u5206\u5b50\u306fCMC\u3088\u308a\u4f4e\u3044\u6fc3\u5ea6\u3067\u3082\u6c34\u6eb6\u6027\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u7d50\u5408\u3057\u307e\u3059\u3002\u754c\u9762\u6d3b\u6027\u5264\u306e\u6fc3\u5ea6\u304c\u5897\u52a0\u3059\u308b\u3068\u3001CMC\u3088\u308a\u4f4e\u3044\u6fc3\u5ea6\u3067\u306f\u30bf\u30f3\u30d1\u30af\u8cea\u3078\u306e\u7d50\u5408\u91cf\u304c\u5897\u52a0\u3057\u307e\u3059\u3002\u754c\u9762\u6d3b\u6027\u5264\u306e\u6fc3\u5ea6\u304cCMC\u3092\u8d85\u3048\u308b\u304f\u3089\u3044\u3067\u306f\u3001\u30e2\u30ce\u30de\u30fc\u5206\u5b50\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u306e\u7d50\u5408\u3068\u3001\u754c\u9762\u6d3b\u6027\u5264\u306e\u4f1a\u5408\uff08\u30df\u30bb\u30eb\u5f62\u6210\uff09\u3068\u304c\u7af6\u5408\u3057(<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic2\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa13_Social_LAB#anchor01\">1<\/a>)\u3001CMC\u3092\u8d85\u3048\u3066\u66f4\u306b\u754c\u9762\u6d3b\u6027\u5264\u306e\u6fc3\u5ea6\u304c\u9ad8\u3044\u72b6\u614b\u3067\u306f\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u7d50\u5408\u3059\u308b\u754c\u9762\u6d3b\u6027\u5264\u306e\u30e2\u30ce\u30de\u30fc\u5206\u5b50\u306e\u6570\u306f\u5897\u52a0\u305b\u305a\u4e00\u5b9a\u306b\u306a\u308a\u307e\u3059\u3002\u3057\u305f\u304c\u3063\u3066\u3001<strong>\u9069\u5f53\u306a\u754c\u9762\u6d3b\u6027\u5264\u3092\u7528\u3044\u3066\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u5341\u5206\u306b\u53ef\u6eb6\u5316\u3059\u308b\u305f\u3081\u306b\u306f\u3001\u5c11\u306a\u304f\u3068\u3082\u305d\u306e\u754c\u9762\u6d3b\u6027\u5264\u306eCMC\u3088\u308a\u3082\u9ad8\u3044\u6fc3\u5ea6\u3092\u30bf\u30f3\u30d1\u30af\u8cea\u6eb6\u6db2\u306b\u6dfb\u52a0\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002<\/strong>\u3057\u304b\u3057\u3001CMC\u3092\u8d85\u3048\u308b\u6fc3\u5ea6\u3067\u3001\u306a\u304a\u304b\u3064\u6eb6\u6db2\u4e2d\u306b\u5b58\u5728\u3059\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u30e2\u30ce\u30de\u30fc\u5206\u5b50\u3068\u306e\u7d50\u5408\u304c\u98fd\u548c\u3059\u308b\u6fc3\u5ea6\u4ee5\u4e0a\u3001\u904e\u5270\u306b\u754c\u9762\u6d3b\u6027\u5264\u304c\u6dfb\u52a0\u3055\u308c\u3066\u3082\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u7d50\u5408\u3059\u308b\u30e2\u30ce\u30de\u30fc\u5206\u5b50\u306e\u6fc3\u5ea6\u306f\u5909\u5316\u305b\u305a\uff08\u982d\u6253\u3061\u306b\u306a\u308a\uff09\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u53ef\u6eb6\u5316\u306b\u306f\u305d\u308c\u4ee5\u4e0a\u5f71\u97ff\u3092\u4e0e\u3048\u307e\u305b\u3093\u3002\u3080\u3057\u308d<strong>\u754c\u9762\u6d3b\u6027\u5264\u3092\u904e\u5270\u306b\u4f7f\u7528\u3059\u308b\u3068\u3001\u5f8c\u306b\u9664\u53bb\u3059\u308b\u3053\u3068\u304c\u96e3\u3057\u304f\u306a\u308a\u307e\u3059\u3002<\/strong><\/p>\n<p>\u754c\u9762\u6d3b\u6027\u5264\u306e\u9664\u53bb\u306b\u3064\u3044\u3066\u306f\u5f8c\u8ff0\u3057\u307e\u3059\u304c\u3001<strong>\u4e00\u822c\u7684\u306a\u9664\u53bb\u6cd5\u3068\u3057\u3066\u7528\u3044\u3089\u308c\u308b\u900f\u6790\u3067\u306f\u3001\u754c\u9762\u6d3b\u6027\u5264\u306e\u30df\u30bb\u30eb\u306e\u30b5\u30a4\u30ba\u304c\u900f\u6790\u819c\u306e\u30dd\u30a2\u30b5\u30a4\u30ba\u3088\u308a\u3082\u5927\u304d\u3044\u3068\u9664\u53bb\u304c\u56f0\u96e3\u3067\u3059\u3002<\/strong>\u754c\u9762\u6d3b\u6027\u5264\u3092\u9664\u53bb\u3059\u308b\u305f\u3081\u306b\u306fCMC\u3088\u308a\u4f4e\u3044\u6fc3\u5ea6\u306b\u5e0c\u91c8\u3057\u3066\u754c\u9762\u6d3b\u6027\u5264\u3092\u30e2\u30ce\u30de\u30fc\u5316\u3057\u3001\u900f\u6790\u819c\u3092\u901a\u904e\u3067\u304d\u308b\u30b5\u30a4\u30ba\u306b\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u3053\u306e\u3068\u304d\u754c\u9762\u6d3b\u6027\u5264\u6fc3\u5ea6\u304c\u6975\u7aef\u306b\u9ad8\u3044\u3068\u3001\u5e0c\u91c8\u7387\u3082\u975e\u5e38\u306b\u9ad8\u304f\u306a\u308b\u3060\u3051\u3067\u306a\u304f\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u904e\u5270\u306b\u5e0c\u91c8\u3055\u308c\u308b\u305f\u3081\u73fe\u5b9f\u7684\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u5177\u4f53\u4f8b\u3068\u3057\u3066\u30011%\u7a0b\u5ea6\u306eNP-40\u3092\u7528\u3044\u3066\u8abf\u88fd\u3057\u305f\u7d30\u80de\u30e9\u30a4\u30bb\u30fc\u30c8\u304b\u3089NP-40\u3092\u9664\u53bb\u3059\u308b\u5834\u5408\u3001NP-40\u306eCMC\u306f0.0179%\u306e\u305f\u3081\u7d30\u80de\u30e9\u30a4\u30bb\u30fc\u30c8\u3092\u5c11\u306a\u304f\u3068\u308255\u500d\u4ee5\u4e0a\u306b\u5e0c\u91c8\u3057\u306a\u3051\u308c\u3070\u52b9\u7387\u3088\u304f\u9664\u53bb\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u305b\u3093\u3002\u3053\u306e\u3088\u3046\u306b\u754c\u9762\u6d3b\u6027\u5264\u3092\u5f8c\u3067\u9664\u53bb\u3059\u308b\u5834\u5408\u306b\u306f\u3001\u3042\u3089\u304b\u3058\u3081CMC\u306e\u9ad8\u3044\u754c\u9762\u6d3b\u6027\u5264\u3092\u9078\u629e\u3059\u308b\u3053\u3068\u3082\u91cd\u8981\u306b\u306a\u308a\u307e\u3059\u3002<\/p>\n<p>\u751f\u4f53\u819c\u306b\u5b58\u5728\u3059\u308b\u819c\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u53ef\u6eb6\u5316\u306f\u3001\u754c\u9762\u6d3b\u6027\u5264\u306e\u30e2\u30ce\u30de\u30fc\u5206\u5b50\u304c\u751f\u4f53\u819c\u306e\u8102\u8cea\u4e8c\u91cd\u5c64\u5185\u306b\u5206\u3051\u5165\u308b\u3053\u3068\u304b\u3089\u306f\u3058\u307e\u308a\u307e\u3059\u3002\u819c\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u53ef\u6eb6\u5316\u30b9\u30c6\u30c3\u30d7\u306f\u3001\u4f7f\u7528\u3059\u308b\u754c\u9762\u6d3b\u6027\u5264\u306e\u91cf\u306b\u3088\u3063\u3066\u3044\u304f\u3064\u304b\u306e\u30b9\u30c6\u30fc\u30b8\u306b\u5206\u3051\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\uff08\u88681\uff09\u3002\u6700\u521d\u306e\u30b9\u30c6\u30fc\u30b8\u3067\u306f\u754c\u9762\u6d3b\u6027\u5264\u304c\u751f\u4f53\u819c\u306b\u5165\u308a\u8fbc\u307f\u307e\u3059\u3002\u754c\u9762\u6d3b\u6027\u5264\u3068\u751f\u4f53\u819c\u8102\u8cea\u3068\u306e\u30e2\u30eb\u6bd40.1:1\uff5e1:1\u306b\u304a\u3044\u3066\u306f\u3001\u8102\u8cea\u4e8c\u91cd\u5c64\u306f\uff08\u30bb\u30df\uff09\u30a4\u30f3\u30bf\u30af\u30c8\u306a\u72b6\u614b\u3067\u5b58\u5728\u3057\u307e\u3059\uff08\u4e00\u90e8\u306e\u819c\u30bf\u30f3\u30d1\u30af\u8cea\u306f\u3053\u306e\u3068\u304d\u9078\u629e\u7684\u306b\u62bd\u51fa\u3055\u308c\u307e\u3059\uff09\u3002\u30e2\u30eb\u6bd4\u304c2:1\u306b\u5897\u52a0\u3059\u308b\u3068\u751f\u4f53\u819c\u306f\u53ef\u6eb6\u5316\u3055\u308c\u3066\u754c\u9762\u6d3b\u6027\u5264\u3068\u6df7\u5408\u30df\u30bb\u30eb\u3092\u5f62\u6210\u3057\u307e\u3059\u3002\u6df7\u5408\u30df\u30bb\u30eb\u306b\u306f\u3001\u30ea\u30f3\u8102\u8cea\u3068\u754c\u9762\u6d3b\u6027\u5264\u3067\u5f62\u6210\u3055\u308c\u308b\u30df\u30bb\u30eb\u3001\u754c\u9762\u6d3b\u6027\u5264\uff0d\u30bf\u30f3\u30d1\u30af\u8cea\u8907\u5408\u4f53\u306e\u30df\u30bb\u30eb\u3001\u8102\u8cea\uff0d\u754c\u9762\u6d3b\u6027\u5264\uff0d\u30bf\u30f3\u30d1\u30af\u8cea\u8907\u5408\u4f53\u306e\u30df\u30bb\u30eb\u304c\u5b58\u5728\u3057\u307e\u3059\u3002\u30e2\u30eb\u6bd4\u304c10:1\u306b\u306a\u308b\u3068\u3001\u751f\u4f53\u819c\u8102\u8cea-\u30bf\u30f3\u30d1\u30af\u8cea\u9593\u306e\u76f8\u4e92\u4f5c\u7528\u306f\u307b\u3068\u3093\u3069\u754c\u9762\u6d3b\u6027\u5264-\u30bf\u30f3\u30d1\u30af\u8cea\u9593\u306e\u76f8\u4e92\u4f5c\u7528\u306b\u7f6e\u304d\u63db\u308f\u308a\u307e\u3059\u3002<\/p>\n<table>\n<tbody>\n<tr>\n<td style=\"text-align: center\"><strong>\u754c\u9762\u6d3b\u6027\u5264\uff1a\u751f\u4f53\u819c\u8102\u8cea<br \/>\n<\/strong><strong>\uff08\u30e2\u30eb\u6bd4\uff09<\/strong><\/td>\n<td><strong>\u72b6\u614b<\/strong><\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\">0.1 : 1\u3000\u301c\u30001 : 1<\/td>\n<td>\u754c\u9762\u6d3b\u6027\u5264\u304c\u5165\u308a\u8fbc\u3093\u3060\u30bb\u30df\u30a4\u30f3\u30bf\u30af\u30c8\u306a\u8102\u8cea\u4e8c\u91cd\u5c64<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\">2 : 1<\/td>\n<td>\u30ea\u30f3\u8102\u8cea-\u754c\u9762\u6d3b\u6027\u5264\u3001\u30ea\u30f3\u8102\u8cea-\u754c\u9762\u6d3b\u6027\u5264-\u30bf\u30f3\u30d1\u30af\u8cea\u3001\u30bf\u30f3\u30d1\u30af\u8cea-\u754c\u9762\u6d3b\u6027\u5264<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center\">10 : 1<\/td>\n<td>\u30ea\u30f3\u8102\u8cea-\u754c\u9762\u6d3b\u6027\u5264\u3001\u30bf\u30f3\u30d1\u30af\u8cea-\u754c\u9762\u6d3b\u6027\u5264<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong>\u30bf\u30f3\u30d1\u30af\u8cea\u62bd\u51fa\u306b\u9069\u3057\u305f\u754c\u9762\u6d3b\u6027\u5264\u306e\u91cf\u306f\u3001\u4f7f\u7528\u3059\u308b\u754c\u9762\u6d3b\u6027\u5264\u306eCMC\u5024\u3084\u3001\u30df\u30bb\u30eb\u3042\u305f\u308a\u306e\u754c\u9762\u6d3b\u6027\u5264\u5206\u5b50\u306e\u6570\u3092\u8868\u3059\u30df\u30bb\u30eb\u4f1a\u5408\u6570\uff08aggregation number\uff09\u3001\u53cd\u5fdc\u6e29\u5ea6\u3001\u819c\u306e\u6027\u8cea\u306b\u3088\u3063\u3066\u7570\u306a\u308a\u307e\u3059\u3002<\/strong>\u307e\u305f\u3001\u901a\u5e38\u30df\u30bb\u30eb1\u500b\u3042\u305f\u308a1\u5206\u5b50\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u542b\u307e\u308c\u308b\u305f\u3081\u3001\u819c\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u53ef\u6eb6\u5316\u30d0\u30c3\u30d5\u30a1\u30fc\u306b\u306f\u819c\u30bf\u30f3\u30d1\u30af\u8cea1\u5206\u5b50\u3042\u305f\u308a\u30df\u30bb\u30eb1\u500b\u5206\u4ee5\u4e0a\u306e\u6fc3\u5ea6\u306e\u754c\u9762\u6d3b\u6027\u5264\u3092\u6dfb\u52a0\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059(<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic2\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa13_Social_LAB#anchor02\">2<\/a>)\u3002<\/p>\n<h2>\u53ef\u6eb6\u5316\u30bf\u30f3\u30d1\u30af\u8cea\u304b\u3089\u306e\u754c\u9762\u6d3b\u6027\u5264\u306e\u9664\u53bb<\/h2>\n<p>\u754c\u9762\u6d3b\u6027\u5264\u306f\u7d30\u80de\u6eb6\u89e3\u3084\u819c\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u62bd\u51fa\u306b\u6709\u52b9\u3067\u3059\u304c\u3001\u62bd\u51fa\u5f8c\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u7528\u3044\u308b\u4e0b\u6d41\u306e\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u3084\u5b9f\u9a13\u3067\u306f\u754c\u9762\u6d3b\u6027\u5264\u3092\u9664\u53bb\u3057\u306a\u3051\u308c\u3070\u306a\u3089\u306a\u3044\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u3002\u4f8b\u3048\u3070\u3001\u754c\u9762\u6d3b\u6027\u5264\u3067\u53ef\u6eb6\u5316\u3055\u308c\u305f\u819c\u30bf\u30f3\u30d1\u30af\u8cea\u306f\u8102\u8cea\u3068\u306e\u76f8\u4e92\u4f5c\u7528\u304c\u7834\u58ca\u3055\u308c\u305f\u3053\u3068\u306b\u3088\u308a\u6d3b\u6027\u3092\u5931\u3046\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u304c\u3001\u62bd\u51fa\u6642\u306b\u4f7f\u7528\u3057\u305f\u754c\u9762\u6d3b\u6027\u5264\u3092\u30ea\u30f3\u8102\u8cea\u3084\u751f\u4f53\u819c\u8102\u8cea\u3068\u985e\u4f3c\u3057\u305f\u8102\u8cea\u6df7\u5408\u6db2\u3067\u7f6e\u304d\u63db\u3048\u308b\u3053\u3068\u306b\u3088\u3063\u3066\u518d\u3073\u6d3b\u6027\u3092\u3082\u3064\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u307e\u305f\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u6d3b\u6027\u304c\u554f\u984c\u306b\u306a\u3089\u306a\u3044\u5834\u5408\u3067\u3082\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u3084\u30b2\u30eb\u96fb\u6c17\u6cf3\u52d5\u306b\u4f9b\u3059\u308b\u969b\u306b\u3001\u62bd\u51fa\u6642\u306b\u4f7f\u7528\u3057\u305f\u754c\u9762\u6d3b\u6027\u5264\u304c\u9069\u3057\u3066\u304a\u3089\u305a\u9664\u53bb\u3059\u308b\u5fc5\u8981\u304c\u3042\u308b\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u3002<br \/>\n<strong>\u900f\u6790\u6cd5\u306fCMC\u304c\u9ad8\u3044\u754c\u9762\u6d3b\u6027\u5264\u3084\u30df\u30bb\u30eb\u4f1a\u5408\u6570\u304c\u5c0f\u3055\u3044\u754c\u9762\u6d3b\u6027\u5264\u306e\u9664\u53bb\u306b\u6709\u7528\u3067\u3059\u3002<\/strong>\u53cd\u5bfe\u306bCMC\u304c\u4f4e\u3044\u754c\u9762\u6d3b\u6027\u5264\u3084\u30df\u30bb\u30eb\u4f1a\u5408\u6570\u304c\u5927\u304d\u3044\u754c\u9762\u6d3b\u6027\u5264\u306f\u3001\u307b\u3068\u3093\u3069\u304c\u900f\u6790\u819c\u306e\u30dd\u30a2\u30b5\u30a4\u30ba\u3088\u308a\u3082\u5927\u304d\u3044\u30df\u30bb\u30eb\u72b6\u614b\u3067\u5b58\u5728\u3057\u3066\u304a\u308a\u3001\u5171\u5b58\u3057\u3066\u3044\u308b\u30e2\u30ce\u30de\u30fc\u3057\u304b\u52b9\u7387\u7684\u306b\u6392\u9664\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u305b\u3093\u3002\u88682\u306b\u306f\u4ee3\u8868\u7684\u306a\u754c\u9762\u6d3b\u6027\u5264\u306eCMC\u3084\u30df\u30bb\u30eb\u4f1a\u5408\u6570\u3068\u3068\u3082\u306b\u900f\u6790\u306b\u3088\u308b\u9664\u53bb\u306e\u53ef\u80fd\u6027\u3092\u307e\u3068\u3081\u307e\u3057\u305f\u3002<\/p>\n<p><strong>\u30a4\u30aa\u30f3\u4ea4\u63db\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u3082\u754c\u9762\u6d3b\u6027\u5264\u306e\u9664\u53bb\u306b\u6709\u7528\u3067\u3059\u3002<\/strong>\u975e\u30a4\u30aa\u30f3\u6027\u307e\u305f\u306f\u4e21\u30a4\u30aa\u30f3\u6027\u754c\u9762\u6d3b\u6027\u5264\u306e\u30df\u30bb\u30eb\u306b\u3064\u3044\u3066\u3001\u30a4\u30aa\u30f3\u4ea4\u63db\u6a39\u8102\u306b\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u542b\u3080\u30df\u30bb\u30eb\u3092\u5438\u7740\u3055\u305b\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u542b\u307e\u306a\u3044\u30df\u30bb\u30eb\u3092\u7d20\u901a\u308a\u3055\u305b\u308b\u3053\u3068\u3067\u5206\u96e2\u3067\u304d\u307e\u3059(<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic2\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa13_Social_LAB#anchor03\">3<\/a>)\u3002\u6a39\u8102\u306b\u5438\u7740\u3057\u305f\u30bf\u30f3\u30d1\u30af\u8cea\u306f\u30a4\u30aa\u30f3\u5f37\u5ea6\u3084pH\u306e\u7570\u306a\u308b\u30d0\u30c3\u30d5\u30a1\u30fc\u3067\u6eb6\u51fa\u3059\u308b\u304b\u3001\u307e\u305f\u306f\u5225\u306e\u754c\u9762\u6d3b\u6027\u5264\u306b\u7f6e\u304d\u63db\u3048\u3066\u6eb6\u51fa\u3057\u307e\u3059\u3002<\/p>\n<p>\u305d\u306e\u4ed6\u306b\u3001\u30b7\u30e7\u7cd6\u5bc6\u5ea6\u52fe\u914d\u3092\u5229\u7528\u3057\u305f\u65b9\u6cd5(<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic2\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa13_Social_LAB#anchor04\">4<\/a>)\u3001\u30a2\u30bb\u30c8\u30f3\u6c88\u6bbf\u3092\u5229\u7528\u3059\u308b\u65b9\u6cd5\u3001\u30b2\u30eb\u6ffe\u904e\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u304c\u5229\u7528\u3055\u308c\u307e\u3059\u3002\u4f8b\u3048\u3070\u3001\u5f0a\u793e\u88fd\u54c1\u306e<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/20308?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa13_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">SDS-Out Precipitation Kit<\/a>\u306f\u30de\u30a4\u30af\u30ed\u30b9\u30d4\u30f3\u30c1\u30e5\u30fc\u30d6\u3092\u7528\u3044\u3066\u3001\u5c0f\u5bb9\u91cf\uff08380 \u00b5l\uff09\u306e\u30b5\u30f3\u30d7\u30eb\u304b\u3089SDS\u3092\u9664\u53bb\u3059\u308b\u305f\u3081\u306e\u30ad\u30c3\u30c8\u3067\u3059\u3002<\/p>\n<h2>\u754c\u9762\u6d3b\u6027\u5264\u306e\u7d14\u5ea6<\/h2>\n<p>\u3055\u307e\u3056\u307e\u306a\u7a2e\u985e\u306e\u754c\u9762\u6d3b\u6027\u5264\u304c\u5e02\u8ca9\u3055\u308c\u3066\u304a\u308a\u3001\u591a\u304f\u306e\u7814\u7a76\u5ba4\u3067\u754c\u9762\u6d3b\u6027\u5264\u304c\u5229\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u3057\u304b\u3057\u3001\u754c\u9762\u6d3b\u6027\u5264\u306e\u7d14\u5ea6\u3084\u5b89\u5b9a\u6027\u306b\u3064\u3044\u3066\u306f\u4e00\u822c\u7684\u306b\u3042\u307e\u308a\u91cd\u8981\u8996\u3055\u308c\u3066\u3044\u307e\u305b\u3093\u3002\u591a\u304f\u306e\u5834\u5408\u3001\u5e02\u8ca9\u306e\u754c\u9762\u6d3b\u6027\u5264\u306b\u306f\u4e0d\u7d14\u7269\u304c\u542b\u307e\u308c\u3066\u3044\u307e\u3059\u3002\u4e0d\u7d14\u7269\u306e\u4e2d\u3067\u3082\u7279\u306b\u975e\u30a4\u30aa\u30f3\u6027\u754c\u9762\u6d3b\u6027\u5264\u306b\u3088\u304f\u542b\u307e\u308c\u3066\u3044\u308b\u904e\u9178\u5316\u7269\u306f\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u4e0d\u6d3b\u6027\u5316\u3059\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059(<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic2\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa13_Social_LAB#anchor05\">5<\/a>\u3001<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic2\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa13_Social_LAB#anchor06\">6<\/a>)\u3002\u307e\u305f\u3001\u4e0d\u7d14\u7269\u3092\u542b\u3093\u3067\u3044\u308b\u305f\u3081\u306b\u7a7a\u6c17\u3084\u7d2b\u5916\u5149\u306b\u3055\u3089\u3055\u308c\u308b\u3053\u3068\u3067\u3059\u3050\u306b\u9178\u5316\u3057\u3066\u3057\u307e\u3044\u3001\u53ef\u6eb6\u5316\u80fd\u304c\u4f4e\u4e0b\u3059\u308b\u754c\u9762\u6d3b\u6027\u5264\u3082\u3042\u308a\u307e\u3059\u3002\u5f0a\u793e\u88fd\u54c1\u306e<a href=\"http:\/\/www.thermofisher.com\/search\/global\/searchAction.action?query=detergents+%26+solubilizers&amp;navigator=seproductcategorynavigator&amp;modifier=Detergents+%26amp%3B+Solubilizers\" target=\"_blank\" rel=\"noopener noreferrer\">\u754c\u9762\u6d3b\u6027\u5264\u30b7\u30ea\u30fc\u30ba<\/a>\u3067\u306f\u3001\u9ad8\u7d14\u5ea6\u3067\u904e\u9178\u5316\u7269\u3092\u307b\u3068\u3093\u3069\u542b\u307e\u306a\u3044\u88fd\u54c1\u3092\u30e9\u30a4\u30f3\u30ca\u30c3\u30d7\u3057\u3066\u3044\u307e\u3059\u3002\u3053\u308c\u3089\u306e\u88fd\u54c1\u306f\u3001\u7a92\u7d20\u30ac\u30b9\u5145\u586b\u3057\u305f\u900f\u660e\u306e\u30ac\u30e9\u30b9\u30a2\u30f3\u30d7\u30eb\u306b\u500b\u5225\u5305\u88c5\u3057\u305f\u9ad8\u54c1\u8cea\u306e\u88fd\u54c1\u3068\u3057\u3066\u63d0\u4f9b\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<table>\n<tbody>\n<tr>\n<td><strong>\u754c\u9762\u6d3b\u6027\u5264<\/strong><\/td>\n<td><strong>\u5206\u985e<\/strong><\/td>\n<td><strong>\u30df\u30bb\u30eb\u4f1a\u5408\u6570<\/strong><\/td>\n<td><strong>\u30df\u30bb\u30eb\u5206\u5b50\u91cf (Da)<\/strong><\/td>\n<td><strong>\u5206\u5b50\u91cf (Da)<\/strong><\/td>\n<td><strong>CMC (mM)<\/strong><\/td>\n<td><strong>CMC (% w\/v)<\/strong><\/td>\n<td><strong>\u66c7\u308a\u70b9 (\u2103)<\/strong><\/td>\n<td><strong>\u900f\u6790\u306b\u3088\u308b\u9664\u53bb<\/strong>*<\/td>\n<\/tr>\n<tr>\n<td>Triton X-100<\/td>\n<td>\u975e\u30a4\u30aa\u30f3\u6027<\/td>\n<td>140<\/td>\n<td>90,000<\/td>\n<td>647<\/td>\n<td>0.24<\/td>\n<td>0.0155<\/td>\n<td>64<\/td>\n<td>\u56f0\u96e3<\/td>\n<\/tr>\n<tr>\n<td>Triton X-114<\/td>\n<td>\u975e\u30a4\u30aa\u30f3\u6027<\/td>\n<td>&#8211;<\/td>\n<td>&#8211;<\/td>\n<td>537<\/td>\n<td>0.21<\/td>\n<td>0.0113<\/td>\n<td>23<\/td>\n<td>\u56f0\u96e3<\/td>\n<\/tr>\n<tr>\n<td>NP-40<\/td>\n<td>\u975e\u30a4\u30aa\u30f3\u6027<\/td>\n<td>149<\/td>\n<td>90,000<\/td>\n<td>617<\/td>\n<td>0.29<\/td>\n<td>0.0179<\/td>\n<td>80<\/td>\n<td>\u56f0\u96e3<\/td>\n<\/tr>\n<tr>\n<td>Brij-35<\/td>\n<td>\u975e\u30a4\u30aa\u30f3\u6027<\/td>\n<td>40<\/td>\n<td>49,000<\/td>\n<td>1225<\/td>\n<td>0.09<\/td>\n<td>0.1103<\/td>\n<td>&gt;100<\/td>\n<td>\u56f0\u96e3<\/td>\n<\/tr>\n<tr>\n<td>Brij-58<\/td>\n<td>\u975e\u30a4\u30aa\u30f3\u6027<\/td>\n<td>70<\/td>\n<td>82,000<\/td>\n<td>1120<\/td>\n<td>0.077<\/td>\n<td>0.0086<\/td>\n<td>&gt;100<\/td>\n<td>\u56f0\u96e3<\/td>\n<\/tr>\n<tr>\n<td>Tween-20<\/td>\n<td>\u975e\u30a4\u30aa\u30f3\u6027<\/td>\n<td>&#8211;<\/td>\n<td>&#8211;<\/td>\n<td>1228<\/td>\n<td>0.06<\/td>\n<td>0.0074<\/td>\n<td>95<\/td>\n<td>\u56f0\u96e3<\/td>\n<\/tr>\n<tr>\n<td>Tween-80<\/td>\n<td>\u975e\u30a4\u30aa\u30f3\u6027<\/td>\n<td>60<\/td>\n<td>76,000<\/td>\n<td>1310<\/td>\n<td>0.012<\/td>\n<td>0.0016<\/td>\n<td>&#8211;<\/td>\n<td>\u56f0\u96e3<\/td>\n<\/tr>\n<tr>\n<td>Octyl Glucoside<\/td>\n<td>\u975e\u30a4\u30aa\u30f3\u6027<\/td>\n<td>27<\/td>\n<td>8,000<\/td>\n<td>292<\/td>\n<td>23 ~ 25<\/td>\n<td>0.6716 ~ 0.7300<\/td>\n<td>&gt;100<\/td>\n<td>\u5bb9\u6613<\/td>\n<\/tr>\n<tr>\n<td>Octylthio Glucoside<\/td>\n<td>\u975e\u30a4\u30aa\u30f3\u6027<\/td>\n<td>&#8211;<\/td>\n<td>&#8211;<\/td>\n<td>308<\/td>\n<td>9<\/td>\n<td>0.2772<\/td>\n<td>&gt;100<\/td>\n<td>\u5bb9\u6613<\/td>\n<\/tr>\n<tr>\n<td>SDS<\/td>\n<td>\u9670\u30a4\u30aa\u30f3\u6027<\/td>\n<td>62<\/td>\n<td>18,000<\/td>\n<td>288<\/td>\n<td>6 ~ 8<\/td>\n<td>0.1728 ~ 2304<\/td>\n<td>&gt;100<\/td>\n<td>\u5bb9\u6613<\/td>\n<\/tr>\n<tr>\n<td>CHAPS<\/td>\n<td>\u4e21\u30a4\u30aa\u30f3\u6027<\/td>\n<td>10<\/td>\n<td>6,149<\/td>\n<td>615<\/td>\n<td>8 ~ 10<\/td>\n<td>0.4920 ~ 0.6150<\/td>\n<td>&gt;100<\/td>\n<td>\u5bb9\u6613<\/td>\n<\/tr>\n<tr>\n<td>CHAPSO<\/td>\n<td>\u4e21\u30a4\u30aa\u30f3\u6027<\/td>\n<td>11<\/td>\n<td>6,940<\/td>\n<td>631<\/td>\n<td>8 ~ 10<\/td>\n<td>0.5048<\/td>\n<td>90<\/td>\n<td>\u5bb9\u6613<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><span style=\"font-size: 8pt\">* \u900f\u6790\u306b\u3088\u308b\u9664\u53bb\u304c\u56f0\u96e3\u3068\u3057\u3066\u3044\u308b\u754c\u9762\u6d3b\u6027\u5264\u3067\u3082\u3001CMC\u3088\u308a\u4f4e\u3044\u6fc3\u5ea6\u306b\u5e0c\u91c8\u3057\u3066\u30e2\u30ce\u30de\u30fc\u5316\u3059\u308b\u3053\u3068\u3067\u9664\u53bb\u53ef\u80fd\u3067\u3059\u3002\u307e\u305f\u754c\u9762\u6d3b\u6027\u5264\u306f\u6eb6\u6db2\u4e2d\u3067\u30df\u30bb\u30eb\u3068\u30e2\u30ce\u30de\u30fc\u304c\u5e73\u8861\u72b6\u614b\u3067\u5b58\u5728\u3057\u3066\u3044\u308b\u305f\u3081\u30e2\u30ce\u30de\u30fc\u3092\u9664\u53bb\u3057\u306a\u304c\u3089\u5e73\u8861\u3092\u30e2\u30ce\u30de\u30fc\u5074\u306b\u30b7\u30d5\u30c8\u3055\u305b\u308b\u305f\u3081\u306b\u9577\u6642\u9593\u900f\u6790\u3092\u884c\u3046\u3053\u3068\u3067\u754c\u9762\u6d3b\u6027\u5264\u304c\u9664\u53bb\u3067\u304d\u308b\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u3002<\/span><\/p>\n<h2>\u6700\u5f8c\u306b<\/h2>\n<p>\u754c\u9762\u6d3b\u6027\u5264\u306f\u3001\u30d0\u30a4\u30aa\u5b9f\u9a13\u306b\u304a\u3044\u3066\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u53ef\u6eb6\u5316\u3084\u5909\u6027\u306e\u307b\u304b\u7d30\u80de\u304b\u3089\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u62bd\u51fa\u306a\u3069\u306b\u5e45\u5e83\u304f\u5229\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u5b9f\u9a13\u306e\u76ee\u7684\u3084\u4e0b\u6d41\u306e\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306b\u5408\u308f\u305b\u3066\u754c\u9762\u6d3b\u6027\u5264\u3092\u9078\u629e\u3059\u308b\u305f\u3081\u306b\u306f\u305d\u308c\u305e\u308c\u306e\u7279\u6027\u3092\u7406\u89e3\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u3053\u3053\u3067\u306f\u754c\u9762\u6d3b\u6027\u5264\u306e\u4e00\u822c\u7684\u306a\u6027\u8cea\u3092\u4e2d\u5fc3\u306b\u3054\u7d39\u4ecb\u3057\u307e\u3057\u305f\u304c\u3001\u30a4\u30aa\u30f3\u6027\u3084\u81e8\u754c\u30df\u30bb\u30eb\u6fc3\u5ea6\uff08CMC\uff09\u306b\u3064\u3044\u3066\u7406\u89e3\u3057\u3066\u304a\u304f\u3060\u3051\u3067\u3082\u4f7f\u7528\u3059\u308b\u754c\u9762\u6d3b\u6027\u5264\u306e\u9078\u629e\u306b\u5f79\u7acb\u3064\u3053\u3068\u3067\u3057\u3087\u3046\uff01<\/p>\n<h2>\u53c2\u8003\u6587\u732e<\/h2>\n<p><a name=\"anchor01\"><\/a>1. <a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/1091302\" target=\"_blank\" rel=\"noopener noreferrer\">Helenius, A. and Simons, K. (1975). Biochim. Biophys. Acta 415, 29-79.<\/a><br \/>\n<a name=\"anchor02\"><\/a>2. <a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/459890\" target=\"_blank\" rel=\"noopener noreferrer\">Helenius, A., et al. (1979). Methods Enzymol. 56, 734-749.<\/a><br \/>\n<a name=\"anchor03\"><\/a>3. <a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/2314241\" target=\"_blank\" rel=\"noopener noreferrer\">Hjelmeland, L.M. (1990). Methods Enzymol. 182, 277-282.<\/a><br \/>\n<a name=\"anchor04\"><\/a>4. <a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/4274560\" target=\"_blank\" rel=\"noopener noreferrer\">Warren, G.B., et al. (1974). Proc. Nat. Acad. Sci. USA 71, 622-626.<\/a><br \/>\n<a name=\"anchor05\"><\/a>5. <a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/7386873\" target=\"_blank\" rel=\"noopener noreferrer\">Chang, H.W. and Bock, E. (1980). Anal. Biochem. 104, 112-117.<\/a><br \/>\n<a name=\"anchor06\"><\/a>6. <a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/7469018\" target=\"_blank\" rel=\"noopener noreferrer\">Ashani, Y. and Catravas, G. (1980). Anal. Biochem. 109, 55-62.<\/a><\/p>\n<h2>\u3010\u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3011\u30bf\u30f3\u30d1\u30af\u8cea\u89e3\u6790\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u30cf\u30f3\u30c9\u30d6\u30c3\u30af<\/h2>\n<p>\u52b9\u7387\u7684\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u62bd\u51fa\u304b\u3089\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u89e3\u6790\u30c4\u30fc\u30eb\u307e\u3067\u3001\u5305\u62ec\u7684\u306b\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u3092\u7d39\u4ecb\u3057\u3066\u304a\u308a\u307e\u3059\u3002PDF\u30d5\u30a1\u30a4\u30eb\u306e\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3092\u3054\u5e0c\u671b\u306e\u65b9\u306f\u3001\u4e0b\u8a18\u30dc\u30bf\u30f3\u3088\u308a\u304a\u7533\u8fbc\u307f\u304f\u3060\u3055\u3044\u3002<\/p>\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/catalogs-protein-form.html?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa13_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9<\/a><\/p>\n<p>&nbsp;<\/p>\n<h2>\u30bf\u30f3\u30d1\u30af\u8cea\u5b9f\u9a13\u95a2\u9023\u3001\u305d\u306e\u4ed6\u306e\u8a18\u4e8b\u306f\u3053\u3061\u3089<\/h2>\n<table style=\"border-color: lightgray;border-collapse: collapse;padding: 20px\" border=\"1\">\n<tbody>\n<tr>\n<td style=\"border-color: lightgray;padding: 20px\">\n<ul>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic1\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa13_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u7b2c1\u56de\u3011\u754c\u9762\u6d3b\u6027\u5264\u3092\u7528\u3044\u305f\u30bf\u30f3\u30d1\u30af\u8cea\u62bd\u51fa<br \/>\n<\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic3\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa13_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u7b2c3\u56de\u3011\u201c\u7dcf\u201d\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u6cd5\u306e\u7a2e\u985e\u3092\u307e\u3068\u3081\u3066\u307f\u305f<br \/>\n<\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic4\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa13_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u7b2c4\u56de\u3011BCA\u6cd5\u3001Bradford\u6cd5\u3001Lowry\u6cd5\u306a\u3069\u3001\u201c\u7dcf\u201d\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u6cd5\u306e\u539f\u7406\u307e\u3068\u3081<br \/>\n<\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic5\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa13_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u7b2c5\u56de\u3011\u900f\u6790\u3001\u30b2\u30eb\u308d\u904e\u3001\u9650\u5916\u308d\u904e\u306a\u3069\u30b5\u30f3\u30d7\u30eb\u4e2d\u306e\u593e\u96d1\u6210\u5206\u306e\u9664\u53bb\u65b9\u6cd5\u307e\u3068\u3081<br \/>\n<\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic19\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa13_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u7b2c19\u56de\u3011\u8cea\u91cf\u5206\u6790\u8a08\u306e\u57fa\u672c\u306e\u300c\u304d\u300d<br \/>\n<\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic20\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa13_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u7b2c20\u56de\u3011\u5b9a\u91cf\u30d7\u30ed\u30c6\u30aa\u30df\u30af\u30b9\u3068\u306f\uff1f<br \/>\n<\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic24_bid_ts\/?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa13_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u201c\u7dcf\u201d\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u6cd5\u306e\u307e\u3068\u3081\uff1a\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\uff01UV\u6cd5\u3084\u86cd\u5149\u6cd5\u3092\u4f7f\u3046\u306e\u306f\u3069\u3093\u306a\u3068\u304d\uff1f<br \/>\n<\/a><\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p>\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u7528\u306b\u306f\u4f7f\u7528\u3044\u305f\u3060\u3051\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u4eca\u56de\u306e\u30c6\u30fc\u30de\u306f\u300c\u754c\u9762\u6d3b\u6027\u5264\u306e\u7279\u6027\u3068\u7a2e\u985e\u300d\u3068\u984c\u3057\u3066\u3001\u754c\u9762\u6d3b\u6027\u5264\u306e\u6982\u8ad6\u3068\u30bf\u30f3\u30d1\u30af\u8cea\u5b9f\u9a13\u3067\u4f7f\u7528\u3059\u308b\u969b\u306b\u7559\u610f\u3059\u3079\u304d\u70b9\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\uff01<\/p>\n","protected":false},"author":988,"featured_media":2050,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[156],"tags":[237,21],"division":[],"class_list":{"0":"post-34","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-mol-bio","8":"tag-protein-experiments","9":"tag-protein-analysis","10":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"<!-- 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