{"id":341,"date":"2024-04-17T00:00:00","date_gmt":"2024-04-16T15:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/digitalpcr2\/"},"modified":"2025-07-01T16:48:12","modified_gmt":"2025-07-01T07:48:12","slug":"digitalpcr2","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/digitalpcr2\/","title":{"rendered":"\u30b7\u30f3\u30d7\u30eb\u3067\u7c21\u5358\uff01\u30c7\u30b8\u30bf\u30ebPCR\u30b7\u30b9\u30c6\u30e0AbsoluteQ\u306e\u30ef\u30fc\u30af\u30d5\u30ed\u30fc"},"content":{"rendered":"<p>\u30c7\u30b8\u30bf\u30ebPCR\u306f\u691c\u91cf\u7dda\u4e0d\u8981\u3067\u7d76\u5bfe\u5b9a\u91cf\u304c\u3067\u304d\u308b\u88c5\u7f6e\u3067\u3059\u3002\u624b\u8efd\u306b\u7d76\u5bfe\u5b9a\u91cf\u3067\u304d\u308b\u3053\u3068\u304b\u3089\u666e\u53ca\u304c\u9032\u3093\u3067\u304a\u308a\u3001\u30a6\u30a4\u30eb\u30b9\u306e\u5b9a\u91cf\u3084\u30ec\u30a2\u30d0\u30ea\u30a2\u30f3\u30c8\u306e\u691c\u51fa\u306a\u3069\u5e45\u5e83\u3044\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306b\u4f7f\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u305d\u306e\u4e00\u65b9\u3067\u30c7\u30b8\u30bf\u30ebPCR\u306b\u3064\u3044\u3066\u300c\u9762\u5012\u300d\u3001\u300c\u96e3\u3057\u3044\u300d\u3068\u3044\u3046\u5370\u8c61\u3092\u304a\u6301\u3061\u306e\u65b9\u3082\u591a\u3044\u306e\u3067\u306f\u306a\u3044\u3067\u3057\u3087\u3046\u304b\u3002\u5f53\u793e\u306e\u30c7\u30b8\u30bf\u30ebPCR\u30b7\u30b9\u30c6\u30e0Applied Biosystems\u2122 QuantStudio\u2122 Absolute Q\u2122\u30c7\u30b8\u30bf\u30ebPCR\u30b7\u30b9\u30c6\u30e0\u306f\u30b7\u30f3\u30d7\u30eb\u3067\u7c21\u5358\u306a\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u306b\u3088\u308a\u3001\u3069\u306a\u305f\u3067\u3082\u624b\u8efd\u306b\u4f7f\u3063\u3066\u3044\u305f\u3060\u3051\u308b\u30b7\u30b9\u30c6\u30e0\u306b\u306a\u3063\u3066\u3044\u307e\u3059\u3002\u3053\u306e\u8a18\u4e8b\u3067\u306f\u5b9f\u969b\u306b\u3069\u306e\u3088\u3046\u306a\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u3067\u6e2c\u5b9a\u304c\u9032\u3080\u306e\u304b\u3092\u8a73\u3057\u304f\u3054\u8aac\u660e\u3057\u307e\u3059\u3002<\/p>\n<p>\u25bc\u3053\u3093\u306a\u65b9\u306b\u304a\u3059\u3059\u3081\u3067\u3059\uff01<br \/>\n\u30fb\u30c7\u30b8\u30bf\u30ebPCR\u306b\u8208\u5473\u304c\u3042\u308b\u65b9<br \/>\n\u30fb\u30c7\u30b8\u30bf\u30ebPCR\u306e\u64cd\u4f5c\u306b\u3064\u3044\u3066\u77e5\u308a\u305f\u3044\u65b9<\/p>\n<h2>\u5168\u4f53\u30ef\u30fc\u30af\u30d5\u30ed\u30fc<\/h2>\n<p>Absolute Q\u30c7\u30b8\u30bf\u30ebPCR\u30b7\u30b9\u30c6\u30e0\u306e\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u306f\u53cd\u5fdc\u6db2\u306e\u8abf\u88fd\u3001\u30d7\u30ec\u30fc\u30c8\u3078\u306e\u30a2\u30d7\u30e9\u30a4\u3001\u88c5\u7f6e\u3067\u306e\u30e9\u30f3\u3001\u7d50\u679c\u89e3\u6790\u306e4\u30b9\u30c6\u30c3\u30d7\u306b\u5206\u3051\u3089\u308c\u307e\u3059\u3002\u5b9f\u969b\u306b\u624b\u3092\u52d5\u304b\u3059\u5fc5\u8981\u304c\u3042\u308b\u306e\u306f\u53cd\u5fdc\u6db2\u306e\u8abf\u88fd\u3068\u30d7\u30ec\u30fc\u30c8\u3078\u306e\u30a2\u30d7\u30e9\u30a4\u306e2\u3064\u306e\u30b9\u30c6\u30c3\u30d7\u306e\u307f\u3067\u3059\u3002\u89e3\u6790\u88c5\u7f6e\u30821\u53f0\u306e\u307f\u3068\u30b7\u30f3\u30d7\u30eb\u306a\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u3068\u306a\u3063\u3066\u304a\u308a\u307e\u3059\u3002<br \/>\n<div id=\"attachment_16910\" style=\"width: 770px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-16910\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2016\/03\/5c6310dc9143f52f1143f3a3c5dedca5-1024x300.jpg\" alt=\"\u56f31. \u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u306e\u30a4\u30e1\u30fc\u30b8\u56f3\" width=\"760\" height=\"223\" class=\"size-large wp-image-16910\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2016\/03\/5c6310dc9143f52f1143f3a3c5dedca5-1024x300.jpg 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2016\/03\/5c6310dc9143f52f1143f3a3c5dedca5-300x88.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2016\/03\/5c6310dc9143f52f1143f3a3c5dedca5-768x225.jpg 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2016\/03\/5c6310dc9143f52f1143f3a3c5dedca5-1536x449.jpg 1536w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2016\/03\/5c6310dc9143f52f1143f3a3c5dedca5-2048x599.jpg 2048w\" sizes=\"auto, (max-width: 760px) 100vw, 760px\" \/><p id=\"caption-attachment-16910\" class=\"wp-caption-text\">\u56f31. \u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u306e\u30a4\u30e1\u30fc\u30b8\u56f3<\/p><\/div><\/p>\n<h2>\u30b9\u30c6\u30c3\u30d71\uff1a\u53cd\u5fdc\u6db2\u306e\u8abf\u88fd<\/h2>\n<p>1\u3064\u306e\u76ee\u306e\u30b9\u30c6\u30c3\u30d7\u3067\u3042\u308b\u53cd\u5fdc\u6db2\u306e\u8abf\u6574\u306b\u3064\u3044\u3066\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002\u53cd\u5fdc\u6db2\u306e\u8abf\u88fd\u65b9\u6cd5\u306f\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306b\u4f3c\u3066\u3044\u307e\u3059\u3002\u9175\u7d20\u3084dNTPs\u304c\u542b\u307e\u308c\u305fMaster Mix\uff08Applied Biosystems\u2122 <a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/jp\/ja\/A52490?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_oc_s00_qpcr34_Social_LAB\" rel=\"noopener\" target=\"_blank\">Absolute Q\u2122 DNA Digital PCR Master Mix (5X)<\/a>\uff09\u3068\u691c\u51fa\u3057\u305f\u3044Target\u3054\u3068\u306b\u7528\u610f\u3057\u305fTaqMan\u2122 Assay\u3001\u30b5\u30f3\u30d7\u30eb\uff08cDNA\u3084gDNA\uff09\u306b\u5bb9\u91cf\u5408\u308f\u305b\u3068\u3057\u3066Nuclease Free Water\u3092\u6dfb\u52a0\u3057\u307e\u3059\u3002\u3053\u308c\u3089\u3092\u6df7\u305c\u5408\u308f\u305b\u308c\u3070\u53cd\u5fdc\u6db2\u304c\u5b8c\u6210\u3057\u307e\u3059\u3002\u6e2c\u5b9a\u3059\u308b\u30b5\u30f3\u30d7\u30eb\u6570\u306b\u3082\u3088\u308a\u307e\u3059\u304c10\u5206\u304b\u30891\u6642\u9593\u7a0b\u5ea6\u3067\u8abf\u88fd\u3067\u304d\u307e\u3059\u3002<br \/>\n<div id=\"attachment_16923\" style=\"width: 375px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-16923\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2024\/04\/ba06801bb1c0800cefd929ed6da8e85e-916x1024.jpg\" alt=\"\u56f32. \u53cd\u5fdc\u6db2\u306e\u7d44\u6210\" width=\"365\" height=\"408\" class=\"size-large wp-image-16923\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2024\/04\/ba06801bb1c0800cefd929ed6da8e85e-916x1024.jpg 916w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2024\/04\/ba06801bb1c0800cefd929ed6da8e85e-268x300.jpg 268w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2024\/04\/ba06801bb1c0800cefd929ed6da8e85e-768x858.jpg 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2024\/04\/ba06801bb1c0800cefd929ed6da8e85e.jpg 1372w\" sizes=\"auto, (max-width: 365px) 100vw, 365px\" \/><p id=\"caption-attachment-16923\" class=\"wp-caption-text\">\u56f32. \u53cd\u5fdc\u6db2\u306e\u7d44\u6210<\/p><\/div><\/p>\n<h2>\u30b9\u30c6\u30c3\u30d72\uff1a\u30d7\u30ec\u30fc\u30c8\u3078\u306e\u30a2\u30d7\u30e9\u30a4<\/h2>\n<p>\u53cd\u5fdc\u6db2\u304c\u8abf\u88fd\u3067\u304d\u305f\u3089\u53cd\u5fdc\u6db2\u3092\u30d7\u30ec\u30fc\u30c8\u3078\u30a2\u30d7\u30e9\u30a4\u3057\u307e\u3059\u3002\u30d7\u30ec\u30fc\u30c8\u306fApplied Biosystems\u2122 <a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/A52865?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_oc_s00_qpcr34_Social_LAB\" rel=\"noopener\" target=\"_blank\">QuantStudio\u2122 Absolute Q\u2122 MAP16 Plate Kit<\/a>\u306b\u542b\u307e\u308c\u308bMAP16\u30d7\u30ec\u30fc\u30c8\u3092\u4f7f\u7528\u3057\u307e\u3059\u3002Absolute Q\u306e\u30d7\u30ec\u30fc\u30c8\u306f1\u30d7\u30ec\u30fc\u30c8\u3067\u6700\u5927\u306716\u53cd\u5fdc\uff08\u30a2\u30ec\u30a4\uff09\u6e2c\u5b9a\u3067\u304d\u30014\u30a2\u30ec\u30a4\u5358\u4f4d\u3067\u6e2c\u5b9a\u53ef\u80fd\u3067\u3059\u3002\u30b5\u30f3\u30d7\u30eb\u6570\u304c\u5c11\u306a\u3044\u969b\u306b\u3082\u30d7\u30ec\u30fc\u30c8\u3092\u7121\u99c4\u306b\u3059\u308b\u3053\u3068\u306a\u304f\u6e2c\u5b9a\u3067\u304d\u307e\u3059\u3002\u5404\u30a2\u30ec\u30a4\u306b\u306f\u53cd\u5fdc\u6db2\u3092\u6dfb\u52a0\u3059\u308b\u305f\u3081\u306e\u30a6\u30a7\u30eb\u304c\u5b58\u5728\u3057\u3066\u3044\u307e\u3059\u3002\u53cd\u5fdc\u6db2\u30929 \u00b5L\u305a\u3064\u30a2\u30d7\u30e9\u30a4\u3057\u3001\u3055\u3089\u306bIsolation Buffer\u309215 \u00b5L\u30a2\u30d7\u30e9\u30a4\u3057\u307e\u3059\u3002<br \/>\n<div id=\"attachment_16912\" style=\"width: 770px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-16912\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2016\/03\/f91e516d6a4353c01582db3c481861a0-1024x517.png\" alt=\"\u56f33. MAP16\u30d7\u30ec\u30fc\u30c8\u3078\u306e\u30a2\u30d7\u30e9\u30a4\" width=\"760\" height=\"384\" class=\"size-large wp-image-16912\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2016\/03\/f91e516d6a4353c01582db3c481861a0-1024x517.png 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2016\/03\/f91e516d6a4353c01582db3c481861a0-300x151.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2016\/03\/f91e516d6a4353c01582db3c481861a0-768x388.png 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2016\/03\/f91e516d6a4353c01582db3c481861a0.png 1064w\" sizes=\"auto, (max-width: 760px) 100vw, 760px\" \/><p id=\"caption-attachment-16912\" class=\"wp-caption-text\">\u56f33. MAP16\u30d7\u30ec\u30fc\u30c8\u3078\u306e\u30a2\u30d7\u30e9\u30a4<\/p><\/div><br \/>\n\u30a2\u30d7\u30e9\u30a4\u304c\u7d42\u308f\u308c\u3070Gasket caps\u3068\u547c\u3070\u308c\u308b\u30b4\u30e0\u88fd\u306e\u30ad\u30e3\u30c3\u30d7\u3092\u3064\u3051\u307e\u3059\u3002\u3053\u308c\u3067\u30d7\u30ec\u30fc\u30c8\u306e\u6e96\u5099\u304c\u5b8c\u4e86\u3067\u3059\u3002\u4f5c\u696d\u6642\u9593\u306f5\u5206\u304b\u308910\u5206\u7a0b\u5ea6\u3067\u3059\u3002<\/p>\n<div id=\"attachment_16913\" style=\"width: 832px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-16913\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2016\/03\/90a1845cb9fd5cdd96578b4284d00fe3.png\" alt=\"\u56f34. Gasket caps\u306e\u53d6\u308a\u4ed8\u3051\" width=\"822\" height=\"408\" class=\"size-full wp-image-16913\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2016\/03\/90a1845cb9fd5cdd96578b4284d00fe3.png 822w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2016\/03\/90a1845cb9fd5cdd96578b4284d00fe3-300x149.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2016\/03\/90a1845cb9fd5cdd96578b4284d00fe3-768x381.png 768w\" sizes=\"auto, (max-width: 822px) 100vw, 822px\" \/><p id=\"caption-attachment-16913\" class=\"wp-caption-text\">\u56f34. Gasket caps\u306e\u53d6\u308a\u4ed8\u3051<\/p><\/div>\n<h2>\u30b9\u30c6\u30c3\u30d73\u30fb4\uff1a\u88c5\u7f6e\u3067\u306e\u30e9\u30f3\u30fb\u30c7\u30fc\u30bf\u89e3\u6790<\/h2>\n<p>Absolute Q\u30c7\u30b8\u30bf\u30ebPCR\u30b7\u30b9\u30c6\u30e0\u306f\u30ed\u30fc\u30c7\u30a3\u30f3\u30b0\u3001PCR\u53cd\u5fdc\u3001\u30c7\u30fc\u30bf\u53d6\u5f97\u306e3\u3064\u306e\u30b9\u30c6\u30c3\u30d7\u30921\u53f0\u3067\u5b9f\u65bd\u3067\u304d\u307e\u3059\u3002\u3064\u307e\u308a\u3001\u88c5\u7f6e\u9593\u3067\u30d7\u30ec\u30fc\u30c8\u3092\u79fb\u52d5\u3055\u305b\u308b\u5fc5\u8981\u304c\u306a\u3044\u305f\u3081\u3001\u8fc5\u901f\u306b\u7d50\u679c\u3092\u5f97\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u300216\u30a2\u30ec\u30a4\u30922\u8272\u3067\u6e2c\u5b9a\u3057\u305f\u5834\u5408\u306e\u53cd\u5fdc\u6642\u9593\u306f\u7d0490\u5206\u3067\u3059\u3002<br \/>\n\u88c5\u7f6e\u3067\u6700\u521d\u306b\u884c\u308f\u308c\u308b\u300c\u30ed\u30fc\u30c7\u30a3\u30f3\u30b0\u300d\u306f20,480\u306e\u30de\u30a4\u30af\u30ed\u30c1\u30e3\u30f3\u30d0\u30fc\u306b\u53cd\u5fdc\u6db2\u3092\u5206\u914d\u3059\u308b\u30b9\u30c6\u30c3\u30d7\u3067\u3059\u3002\u56f35\u306b\u3042\u308b\u3088\u3046\u306b\u3001\u30d7\u30ec\u30fc\u30c8\u4e0a\u306b\u306f\u5fae\u5c0f\u306a\u30de\u30a4\u30af\u30ed\u30c1\u30e3\u30f3\u30d0\u30fc\u304c\u4e26\u3093\u3067\u304a\u308a\u53cd\u5fdc\u6db2\u304c\u5206\u914d\u3055\u308c\u307e\u3059\u3002<br \/>\n<div id=\"attachment_16914\" style=\"width: 770px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-16914\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2016\/03\/ef07c9fc89d5130f0c593b777b346954-1024x278.jpg\" alt=\"\u56f35. MAP16\u30d7\u30ec\u30fc\u30c8\u306e\u62e1\u5927\u56f3\" width=\"760\" height=\"206\" class=\"size-large wp-image-16914\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2016\/03\/ef07c9fc89d5130f0c593b777b346954-1024x278.jpg 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2016\/03\/ef07c9fc89d5130f0c593b777b346954-300x82.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2016\/03\/ef07c9fc89d5130f0c593b777b346954-768x209.jpg 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2016\/03\/ef07c9fc89d5130f0c593b777b346954-1536x417.jpg 1536w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2016\/03\/ef07c9fc89d5130f0c593b777b346954-2048x556.jpg 2048w\" sizes=\"auto, (max-width: 760px) 100vw, 760px\" \/><p id=\"caption-attachment-16914\" class=\"wp-caption-text\">\u56f35. MAP16\u30d7\u30ec\u30fc\u30c8\u306e\u62e1\u5927\u56f3<\/p><\/div><\/p>\n<p>\u300cPCR\u53cd\u5fdc\u300d\u306f\u4e8b\u524d\u306b\u8a2d\u5b9a\u3057\u305fPCR\u30d7\u30ed\u30b0\u30e9\u30e0\u306b\u57fa\u3065\u3044\u3066\u884c\u308f\u308c\u307e\u3059\u3002PCR\u53cd\u5fdc\u306e\u6a19\u6e96\u30d7\u30ed\u30c8\u30b3\u30eb\u306f96 \u2103\u306860 \u2103\u306e2\u30b9\u30c6\u30c3\u30d7\u306e\u30d7\u30ed\u30b0\u30e9\u30e0\u3067\u3059\u3002PCR\u306e\u30d7\u30ed\u30b0\u30e9\u30e0\u306f\u81ea\u7531\u306b\u5909\u66f4\u304c\u3067\u304d\u307e\u3059\u306e\u3067\u3001\u9006\u8ee2\u5199\u53cd\u5fdc\u3092\u542b\u3081\u3066RNA\u304b\u3089\u6e2c\u5b9a\u3059\u308b\u7cfb\u3082\u5b9f\u65bd\u3067\u304d\u307e\u3059\u3002<br \/>\n<div id=\"attachment_16915\" style=\"width: 626px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-16915\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2016\/03\/c6509ad4c8eff8d51c828a43f8ef8b55.png\" alt=\"\u56f36. PCR\u30d7\u30ed\u30b0\u30e9\u30e0\u306e\u4e00\u4f8b\" width=\"616\" height=\"414\" class=\"size-full wp-image-16915\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2016\/03\/c6509ad4c8eff8d51c828a43f8ef8b55.png 616w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2016\/03\/c6509ad4c8eff8d51c828a43f8ef8b55-300x202.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2016\/03\/c6509ad4c8eff8d51c828a43f8ef8b55-96x65.png 96w\" sizes=\"auto, (max-width: 616px) 100vw, 616px\" \/><p id=\"caption-attachment-16915\" class=\"wp-caption-text\">\u56f36. PCR\u30d7\u30ed\u30b0\u30e9\u30e0\u306e\u4e00\u4f8b<\/p><\/div><br \/>\n\u300c\u30c7\u30fc\u30bf\u53d6\u5f97\u300d\u3067\u306f\u9078\u629e\u3057\u305f\u86cd\u5149\u8272\u7d20\u306e\u30c7\u30fc\u30bf\u3092\u56de\u53ce\u3057\u307e\u3059\u3002Quality Check\u7528\u306eROX\u2122\u306e\u4ed6\u306b\u6700\u5927\u30674\u8272\u306e\u86cd\u5149\u8272\u7d20\u306e\u30c7\u30fc\u30bf\u3092\u53d6\u5f97\u3067\u304d\u307e\u3059\u3002\u53cd\u5fdc\u6db21 \u00b5L\u3042\u305f\u308a\u306e\u30b3\u30d4\u30fc\u6570\u304c\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u3067\u81ea\u52d5\u8a08\u7b97\u3055\u308c\u307e\u3059\u306e\u3067\u7d50\u679c\u78ba\u8a8d\u3082\u5bb9\u6613\u3067\u3059\u3002<br \/>\n\u56f37\u306e\u4f8b\u3067\u306fFAM\u2122, VIC\u2122, ABY, CY\u21225\u306e\u30d7\u30ed\u30fc\u30d6\u3092\u4f7f\u7528\u3057\u30011\u30a2\u30ec\u30a4\u30674\u3064\u306e\u30bf\u30fc\u30b2\u30c3\u30c8\u3092\u6e2c\u5b9a\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<div id=\"attachment_16925\" style=\"width: 1748px\" class=\"wp-caption aligncenter\"><a href=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2024\/04\/bf9742a249441bf27f506ee83d675b33.jpg\" target=\"_blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-16925\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2024\/04\/bf9742a249441bf27f506ee83d675b33.jpg\" alt=\"\u56f37. \u89e3\u6790\u7d50\u679c\u306e\u56f3\" width=\"1738\" height=\"336\" class=\"size-full wp-image-16925\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2024\/04\/bf9742a249441bf27f506ee83d675b33.jpg 1738w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2024\/04\/bf9742a249441bf27f506ee83d675b33-300x58.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2024\/04\/bf9742a249441bf27f506ee83d675b33-1024x198.jpg 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2024\/04\/bf9742a249441bf27f506ee83d675b33-768x148.jpg 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2024\/04\/bf9742a249441bf27f506ee83d675b33-1536x297.jpg 1536w\" sizes=\"auto, (max-width: 1738px) 100vw, 1738px\" \/><\/a><p id=\"caption-attachment-16925\" class=\"wp-caption-text\">\u56f37. \u89e3\u6790\u7d50\u679c\u306e\u56f3<br \/>\u203b\u753b\u50cf\u3092\u30af\u30ea\u30c3\u30af\u3059\u308b\u3068\u62e1\u5927\u8868\u793a\u3067\u304d\u307e\u3059<\/p><\/div>\n<h2>\u307e\u3068\u3081<\/h2>\n<p>\u5b9f\u969b\u306e\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u3092\u3054\u89a7\u306b\u306a\u3063\u3066\u3044\u304b\u304c\u3067\u3057\u3087\u3046\u304b\u3001\u60f3\u50cf\u3057\u3066\u3044\u305f\u3088\u308a\u3082\u30b7\u30f3\u30d7\u30eb\u3067\u7c21\u5358\u3068\u601d\u308f\u308c\u305f\u65b9\u3082\u591a\u3044\u306e\u3067\u3057\u3087\u3046\u304b\u3002Absolute Q\u30c7\u30b8\u30bf\u30ebPCR\u30b7\u30b9\u30c6\u30e0\u306f1\u53f0\u3067\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u304c\u3059\u3079\u3066\u5b8c\u7d50\u3059\u308b\u305f\u3081\u3001\u5358\u7d14\u3067\u4f5c\u696d\u6642\u9593\u3082\u5c11\u306a\u3044\u88c5\u7f6e\u3067\u3059\u3002\u305d\u306e\u305f\u3081\u521d\u3081\u3066\u30c7\u30b8\u30bf\u30ebPCR\u3092\u5b9f\u65bd\u3055\u308c\u308b\u65b9\u306b\u304a\u52e7\u3081\u306e\u88c5\u7f6e\u3067\u3059\u3002\u5b9f\u969b\u306b\u591a\u304f\u306e\u30e6\u30fc\u30b6\u30fc\u306e\u65b9\u304b\u3089\u300c\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3068\u540c\u3058\u611f\u899a\u3067\u4f7f\u7528\u3067\u304d\u308b\u300d\u3068\u306e\u304a\u58f0\u3092\u3044\u305f\u3060\u3044\u3066\u3044\u307e\u3059\u3002<br \/>\n\u30d0\u30fc\u30c1\u30e3\u30eb\u30c7\u30e2\u3067\u88c5\u7f6e\u3084\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u306e\u64cd\u4f5c\u3092\u7591\u4f3c\u4f53\u9a13\u3057\u3066\u3044\u305f\u3060\u3051\u307e\u3059\u306e\u3067\u3001\u305c\u3072\u304a\u8a66\u3057\u304f\u3060\u3055\u3044\u3002<\/p>\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/absolute-q-demo-form.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_oc_s00_qpcr34_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">\u304a\u7533\u3057\u8fbc\u307f\u306f\u3053\u3061\u3089<\/a><\/p>\n<p>Absolute Q\u30c7\u30b8\u30bf\u30ebPCR\u30b7\u30b9\u30c6\u30e0\u306e\u7d39\u4ecb\u52d5\u753b\u3082\u516c\u958b\u3057\u3066\u3044\u307e\u3059\uff01<\/p>\n<p><iframe loading=\"lazy\" title=\"Features and Benefits of the Applied Biosystems QuantStudio Absolute Q Digital PCR System\" width=\"760\" height=\"428\" src=\"https:\/\/www.youtube.com\/embed\/j3nhYZJ1iLU?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe><br \/>\n&nbsp;<\/p>\n<h3>\u3010\u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3011\u30c7\u30b8\u30bf\u30ebPCR\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u30ac\u30a4\u30c9<\/h3>\n<p>\u30c7\u30b8\u30bf\u30ebPCR\u306b\u3088\u308b\u5b9a\u91cf\u304c\u6709\u7528\u306a\u8907\u6570\u306e\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306b\u3064\u3044\u3066\u7d39\u4ecb\u3057\u3066\u3044\u307e\u3059\u3002\u3053\u308c\u304b\u3089\u30c7\u30b8\u30bf\u30ebPCR\u5b9f\u9a13\u3092\u8003\u3048\u3066\u3044\u308b\u65b9\u306b\u6700\u9069\u3067\u3059\u3002<\/p>\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/life-science\/dpcr-applications-ebook-jp.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_oc_s00_qpcr34_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u306f\u3053\u3061\u3089<\/a><\/p>\n<p>&nbsp;<br \/>\n\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u7528\u306b\u306f\u4f7f\u7528\u3044\u305f\u3060\u3051\u307e\u305b\u3093\u3002<\/p>\n<p>TaqMan is a treade mark of Roche Molecular Systems, Inc.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u30c7\u30b8\u30bf\u30ebPCR (dPCR)\u3092\u3054\u7d39\u4ecb\u3059\u308b\u30016\u56de\u30b7\u30ea\u30fc\u30ba\u306e\u30d1\u30fc\u30c82\u3067\u3059\u3002\u4eca\u56de\u306f\u3001\u5f0a\u793e\u306eQuantStudio 3D \u30c7\u30b8\u30bf\u30ebPCR \u304c\u3069\u306e\u3088\u3046\u306b\u30b7\u30f3\u30d7\u30eb\u3067\u3001\u304a\u624b\u9803\u3067\u3001\u305d\u3057\u3066\u6700\u3082\u91cd\u8981\u3067\u3042\u308b\u78ba\u5b9f\u306a\u7d76\u5bfe\u5b9a\u91cf\u3092\u5b9f\u73fe\u3057\u3066\u3044\u308b\u304b\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n","protected":false},"author":988,"featured_media":16917,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[156],"tags":[83,44],"division":[],"class_list":{"0":"post-341","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-mol-bio","8":"tag-cancer","9":"tag-digital-pcr","10":"entry"},"_selected_authors":[],"_selected_reviewers":[],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.8 (Yoast SEO v27.8) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>\u30b7\u30f3\u30d7\u30eb\u3067\u7c21\u5358\uff01\u30c7\u30b8\u30bf\u30ebPCR\u30b7\u30b9\u30c6\u30e0AbsoluteQ\u306e\u30ef\u30fc\u30af\u30d5\u30ed\u30fc - Learning at the Bench<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/digitalpcr2\/\" \/>\n<meta property=\"og:locale\" content=\"ja_JP\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"\u30b7\u30f3\u30d7\u30eb\u3067\u7c21\u5358\uff01\u30c7\u30b8\u30bf\u30ebPCR\u30b7\u30b9\u30c6\u30e0AbsoluteQ\u306e\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\" \/>\n<meta property=\"og:description\" content=\"\u30c7\u30b8\u30bf\u30ebPCR 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