{"id":346,"date":"2020-04-01T00:00:00","date_gmt":"2020-03-31T15:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/immunohistochemistry2\/"},"modified":"2025-08-12T17:25:40","modified_gmt":"2025-08-12T08:25:40","slug":"immunohistochemistry2","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/immunohistochemistry2\/","title":{"rendered":"\u306f\u3058\u3081\u3066\u306e\u514d\u75ab\u7d44\u7e54\u5316\u5b66(Immunohistochemistry; IHC)\u307e\u3068\u3081 \u30d7\u30ed\u30fc\u30d3\u30f3\u30b0\u304b\u3089\u53ef\u8996\u5316\u307e\u3067"},"content":{"rendered":"<p><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/immunohistochemistry1\/?cid=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa49_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u524d\u56de<\/a>\u306b\u5f15\u304d\u7d9a\u304d\u4eca\u56de\u306e\u30c6\u30fc\u30de\u3082\u300c<strong>\u7d44\u7e54\u514d\u75ab\u5316\u5b66<\/strong>\u300d\u3067\u3059\u3002<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/immunohistochemistry1\/?cid=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa49_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u524d\u56de<\/a>\u306f\u3001\u7d44\u7e54\u306e\u56fa\u5b9a\u6cd5\u3001\u5207\u7247\u306e\u4f5c\u88fd\u3001\u5185\u56e0\u6027\u306e\u9175\u7d20\u3084\u30d3\u30aa\u30c1\u30f3\u306e\u30de\u30b9\u30af\u3001\u975e\u7279\u7570\u7684\u7d50\u5408\u30b5\u30a4\u30c8\u306e\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u306b\u3064\u3044\u3066\u3054\u7d39\u4ecb\u3057\u307e\u3057\u305f\u304c\u3001\u4eca\u56de\u306f<strong>\u6297\u4f53\u306e\u6a19\u8b58\u3084\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u5316\u306a\u3069\u3092\u542b\u3081\u305f\u6297\u4f53\u306b\u3088\u308b\u6297\u539f\u306e\u53ef\u8996\u5316<\/strong>\u306b\u3064\u3044\u3066\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n<h2>\u30d7\u30ed\u30fc\u30d3\u30f3\u30b0\u3068\u53ef\u8996\u5316<\/h2>\n<h3>\u9175\u7d20\u6297\u4f53\u6cd5<\/h3>\n<p>\u9175\u7d20\u6297\u4f53\u6cd5\u3067\u306fHRP(\u897f\u6d0b\u308f\u3055\u3073\u30d1\u30fc\u30aa\u30ad\u30b7\u30c0\u30fc\u30bc)\u3084AP(\u30a2\u30eb\u30ab\u30ea\u30db\u30b9\u30d5\u30a1\u30bf\u30fc\u30bc)\u3092\u7528\u3044\u307e\u3059\u3002\u57fa\u8cea\u3092\u6a19\u8b58\u9175\u7d20\u3068\u53cd\u5fdc\u3055\u305b\u3066\u3001\u767a\u8272\u6027\u306e\u53cd\u5fdc\u7523\u7269\u3092\u6c88\u964d\u3055\u305b\u308b\u3053\u3068\u3067\u53ef\u8996\u5316\u3057\u307e\u3059\u3002\u67d3\u8272\u3057\u305f\u7d44\u7e54\u3092\u5b89\u5b9a\u306b\u4fdd\u7ba1\u3067\u304d\u308b\u3053\u3068\u3001\u5149\u5b66\u9855\u5fae\u93e1\u306b\u3088\u308a\u7c21\u5358\u306b\u691c\u51fa\u3067\u304d\u308b\u3053\u3068\u304c\u7279\u9577\u3067\u3059\u3002<\/p>\n<h3>\u86cd\u5149\u6297\u4f53\u6cd5<\/h3>\n<p>\u86cd\u5149\u6297\u4f53\u6cd5\u3067\u306f\u6a19\u8b58\u306b\u86cd\u5149\u8272\u7d20\u3092\u7528\u3044\u307e\u3059\u3002<strong>\u6700\u5927\u306e\u7279\u9577\u306f\u30de\u30eb\u30c1\u30ab\u30e9\u30fc\u691c\u51fa\u304c\u53ef\u80fd<\/strong>\u306a\u3053\u3068\u3067\u3059\u3002\u7570\u306a\u308b\u52b1\u8d77\u30fb\u86cd\u5149\u6ce2\u9577\u3092\u3082\u3064\u8907\u6570\u306e\u86cd\u5149\u8272\u7d20\u3092\u7528\u3044\u308b\u3053\u3068\u3067\u3001\u7d44\u7e54\u3084\u7d30\u80de\u5185\u5916\u3067\u767a\u73fe\u3059\u308b\u8907\u6570\u306e\u6297\u539f\u3092\u540c\u6642\u306b\u691c\u51fa\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u3001\u6297\u539f\u9593\u306e\u4f4d\u7f6e\u95a2\u4fc2\uff08\u540c\u4e00\u7d30\u80de\u30fb\u7570\u306a\u308b\u7d30\u80de\u3067\u306e\u767a\u73fe\u306e\u78ba\u8a8d\u306a\u3069\uff09\u3092\u89e3\u6790\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002<\/p>\n<h3>\u5897\u611f<\/h3>\n<p>\u67d3\u8272\u611f\u5ea6\u306f\u6297\u539f\u306b\u7d50\u5408\u3057\u305f\u9175\u7d20\u306e\u5206\u5b50\u6570\u306b\u3088\u308a\u6c7a\u5b9a\u3055\u308c\u308b\u305f\u3081\u3001ABC (Avidin-Biotin Complex)\u6cd5\uff08\u6587\u732e1-3\uff09\u3068\u3088\u3070\u308c\u308b\u624b\u6cd5\u306f\u30b7\u30b0\u30ca\u30eb\u5897\u5e45\u306b\u7406\u60f3\u7684\u3067\u3059\u3002\u30a2\u30d3\u30b8\u30f3-\u30d3\u30aa\u30c1\u30f3\u8907\u5408\u4f53\u304c\u7d44\u7e54\u3078\u306e\u6d78\u900f\u306b\u306f\u5927\u304d\u3059\u304e\u308b\u5834\u5408\u3001\u9175\u7d20\u6a19\u8b58\u30a2\u30d3\u30b8\u30f3\uff08\u307e\u305f\u306f\u30b9\u30c8\u30ec\u30d7\u30c8\u30a2\u30d3\u30b8\u30f3\uff09\u3068\u30d3\u30aa\u30c1\u30f3\u6a19\u8b581\u6b21\u6297\u4f53\u3092\u4f7f\u7528\u3057\u305fLAB (labeled avidin-biotin)\u6cd5\u3068\u3088\u3070\u308c\u308b\u624b\u6cd5(\u6587\u732e4)\u304c\u7528\u3044\u3089\u308c\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002RYD(\u7d30\u80de\u63a5\u7740\u56e0\u5b50\u3068\u306e\u7d50\u5408\u3092\u793a\u3059RGD\u306e\u30df\u30df\u30c3\u30af\u914d\u5217)\u3092\u542b\u307e\u306a\u3044\u975e\u7279\u7570\u7684\u7d50\u5408\u304c\u6700\u5c0f\u5316\u3055\u308c\u305fNeutravidin\uff08\u8131\u30b0\u30ea\u30b3\u30b7\u30eb\u5316\u30a2\u30d3\u30b8\u30f3\uff09\u306b\u95a2\u3057\u3066\u3082\u9175\u7d20\u6a19\u8b58\u7269\u3092\u30e9\u30a4\u30f3\u30ca\u30c3\u30d7\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<h3>\u57fa\u8cea<\/h3>\n<p>\u5f0a\u793e\u88fd\u54c1\u306b\u306f\u7c89\u672b\u72b6\u57fa\u8cea\u3084\u30ad\u30c3\u30c8\u88fd\u54c1\u306a\u3069\u69d8\u3005\u306a\u88fd\u54c1\u304c\u3042\u308a\u307e\u3059\u304c\u3001<strong>HRP\u7528\u306eMetal Enhanced DAB\u3001AP\u7528\u306eNBT\/BCIP\u304c\u6700\u3082\u4e00\u822c\u7684\u306a\u9078\u629e<\/strong>\u3067\u3059\u3002\u6700\u3082\u611f\u5ea6\u304c\u9ad8\u3044\u88fd\u54c1\u306fMetal Enhaced DAB\u6cd5\u306b\u3088\u308b<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/36000?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa49_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Pierce Peroxidase Detection Kit<\/a>\u3067\u3059\u3002\u672c\u6cd5\u3067\u306f\u6697\u8910\u8272\uff5e\u9ed2\u8272\u306e\u6c88\u6bbf\u7269\u3092\u751f\u3058\u308b\u30b3\u30d0\u30eb\u30c8\u3068\u30cb\u30c3\u30b1\u30eb\u306e\u6c88\u7740\u306b\u3088\u308a\u3001DAB\u6cd5\u306b\u6bd4\u307950\u500d\u4ee5\u4e0a\u306e\u611f\u5ea6\u304c\u5f97\u3089\u308c\u308b\u305f\u3081\u3001\u3088\u308a\u9bae\u660e\u306a\u753b\u50cf\u5316\u304c\u53ef\u80fd\u3067\u3059\u3002<\/p>\n<table>\n<caption>\n<p class=\"al-c\">\u8868 \u767a\u8272\u57fa\u8cea\u30fb\u8272\u7d20\u4e00\u89a7<\/p>\n<\/caption>\n<tbody>\n<tr>\n<td>\n<p class=\"al-c\"><strong>\u767a\u8272\u57fa\u8cea\u30fb\u767a\u8272\u8272\u7d20<\/strong><\/p>\n<\/td>\n<td>\n<p class=\"al-c\"><strong>\u9175\u7d20<\/strong><\/p>\n<\/td>\n<td>\n<p class=\"al-c\"><strong>\u611f\u5ea6<\/strong><\/p>\n<\/td>\n<td>\n<p class=\"al-c\"><strong>\u30d5\u30a9\u30fc\u30de\u30c3\u30c8<\/strong><\/p>\n<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/34065?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa49_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Metal Enhanced DAB<\/a><\/td>\n<td>HRP<\/td>\n<td>Highest<\/td>\n<td>\u30ad\u30c3\u30c8 (2\u6db2)<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/34018?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa49_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">1-Step TMB<\/a><\/td>\n<td>HRP<\/td>\n<td>High<\/td>\n<td>1\u6db2<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/34012?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa49_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">1-Step 4-CN<\/a><\/td>\n<td>HRP<\/td>\n<td>High<\/td>\n<td>1\u6db2<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/34000?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa49_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">CN\/DAB<\/a><\/td>\n<td>HRP<\/td>\n<td>High<\/td>\n<td>\u30ad\u30c3\u30c8 (2\u6db2)<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/34001?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa49_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">DAB<\/a><\/td>\n<td>HRP<\/td>\n<td>Medium<\/td>\n<td>\u7c89\u672b<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/34042?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa49_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">1-Step NBT\/BCIP<\/a><\/td>\n<td>AP<\/td>\n<td>High<\/td>\n<td>1\u6db2<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/34070?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa49_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">1-Step NBT\/BCIP + Suppressor<\/a><\/td>\n<td>AP<\/td>\n<td>High<\/td>\n<td>1\u6db2<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/34040?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa49_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">BCIP<\/a><\/td>\n<td>AP<\/td>\n<td>Medium<\/td>\n<td>\u7c89\u672b<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/34035?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa49_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">NBT<\/a><\/td>\n<td>AP<\/td>\n<td>Medium<\/td>\n<td>\u7c89\u672b<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h2>\u4ea4\u53c9\u53cd\u5fdc\u3068<strong>\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9<\/strong><\/h2>\n<p><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/immunohistochemistry1\/?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa49_Social_LAB\" target=\"_blank\">\u524d\u56de<\/a>\u306f\u3001\u7d44\u7e54\u306b\u5185\u5728\u3059\u308b\u9175\u7d20\u3084\u30d3\u30aa\u30c1\u30f3\u306b\u3088\u308b\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9\u306b\u95a2\u3057\u3066\u3054\u7d39\u4ecb\u3057\u307e\u3057\u305f\u304c\u3001<strong>\u7d44\u7e54\u306b\u5185\u5728\u3059\u308b\u8840\u6e05\u86cb\u767d\u8cea\u3082\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9\u306e\u539f\u56e0<\/strong>\u3068\u306a\u308a\u307e\u3059\u3002<strong>\u7279\u306b\u30b0\u30ed\u30d6\u30ea\u30f3\u306b\u7531\u6765\u3059\u308b\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9\u30ce\u30a4\u30ba\u304c\u3088\u304f\u77e5\u3089\u308c\u3066\u3044\u308b<\/strong>\u3053\u3068\u304b\u3089\u3001\u7d44\u7e54\u306b\u5185\u5728\u3059\u308b\u30b0\u30ed\u30d6\u30ea\u30f3\u306b\u7531\u6765\u3059\u308b\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9\u30ce\u30a4\u30ba\u306e\u8efd\u6e1b\u306b\u95a2\u3057\u3066\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n<h3>\u9ad8\u5ea6\u5438\u7740\u6297\u4f53 \uff08ImmunoPure\u30b7\u30ea\u30fc\u30ba\uff09<\/h3>\n<p>\u5f0a\u793e\u306b\u306f\u3001\u30d3\u30aa\u30c1\u30f3\u3001\u86cd\u5149\u3001\u9175\u7d20\u306a\u3069\u3067\u6a19\u8b58\u3055\u308c\u305f\u3001\u30de\u30a6\u30b9\u3084\u30e9\u30d3\u30c3\u30c8\u306a\u3069\u306e\u69d8\u3005\u306a\u30db\u30b9\u30c8\u7531\u6765\u306e\u30dd\u30ea\u30af\u30ed\u30fc\u30ca\u30eb\u6297\u4f53\u304c\u3042\u308a\u307e\u3059\u3002<strong>\u30dd\u30ea\u30af\u30ed\u30fc\u30ca\u30eb\u6297\u4f53\u3068\u7d44\u7e54\u306b\u5185\u5728\u3059\u308b\u8840\u6e05\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u306e\u4ea4\u5dee\u53cd\u5fdc\u306f\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9\u4e0a\u6607\u306e\u539f\u56e0\u3068\u306a\u308b<\/strong>\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u3053\u306e\u3088\u3046\u306a\u3001\u8840\u6e05\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u5bfe\u3057\u3066\u4ea4\u53c9\u53cd\u5fdc\u3092\u793a\u3059\u30dd\u30ea\u30af\u30ed\u30fc\u30ca\u30eb\u6297\u4f53\u3092\u3001\u5404\u7a2e\u306e\u8840\u6e05\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u56fa\u5b9a\u5316\u3057\u305f\u30ab\u30e9\u30e0\u3092\u901a\u904e\u3055\u305b\u3066\u3001\u7cbe\u88fd\u3057\u305f\u6297\u539f\u7279\u7570\u6027\u306e\u9ad8\u3044\u6297\u4f53\u3092\u9ad8\u5ea6\u5438\u7740\u6297\u4f53\u3068\u3088\u3073\u307e\u3059\u3002\u5f0a\u793e\u3067\u306f\u3001\u30d2\u30c8\u3001\u30a6\u30b7\u3001\u30a6\u30de\u3001\u30a6\u30b5\u30ae\u3001\u30d6\u30bf\u306a\u3069\u306e\u8840\u6e05\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u306e\u53cd\u5fdc\u3092\u6700\u5c0f\u9650\u306b\u304a\u3055\u3048\u305f\u9ad8\u5ea6\u5438\u7740\u6297\u4f53\u3082\u3054\u63d0\u4f9b\u3057\u3066\u304a\u308a\u307e\u3059\u3002<\/p>\n<h3>\u6297\u4f53\u306e\u7cbe\u88fd<\/h3>\n<p>\u5e02\u8ca9\u3055\u308c\u3066\u3044\u308b\u6297\u4f53\u306b\u306f\u30a6\u30b7\u8840\u6e05\u30a2\u30eb\u30d6\u30df\u30f3(BSA)\u306a\u3069\u306e\u30ad\u30e3\u30ea\u30a2\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u5b89\u5b9a\u5264\u3068\u3057\u3066\u542b\u307e\u308c\u3066\u3044\u308b\u3053\u3068\u304c\u3042\u308a\u3001<strong>\u3053\u308c\u3089\u306e\u30ad\u30e3\u30ea\u30a2\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9\u30ce\u30a4\u30ba\u306e\u539f\u56e0\u3068\u306a\u308b<\/strong>\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/45206?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa49_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Melon Gel Spin Pufication Kit<\/a>\u3067\u306f\u8840\u6e05\u30bf\u30f3\u30d1\u30af\u8cea\u306f\u30ab\u30e9\u30e0\u306b\u4fdd\u6301\u3001\u7cbe\u88fd\u6297\u4f53\u306f\u4e2d\u6027pH\u306e\u901a\u904e\u30d5\u30e9\u30af\u30b7\u30e7\u30f3\u4e2d\u306b\u56de\u53ce\u3055\u308c\u307e\u3059\u3002\u672c\u6765\u306e\u7528\u9014\u306f\u8840\u6e05\u304b\u3089\u306e\u6297\u4f53\u30ef\u30f3\u30b9\u30c6\u30c3\u30d7\u7cbe\u88fd\u3067\u3059\u304c\u3001\u5e02\u8ca9\u6297\u4f53\u306b\u542b\u307e\u308c\u308b\u30ad\u30e3\u30ea\u30a2\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u9664\u53bb\u306b\u3082\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002<\/p>\n<h3>\u6297\u4f53\u306e\u6a19\u8b58<\/h3>\n<p>\u9069\u5f53\u306a\u5e02\u8ca9\u306e\u6a19\u8b58\u6297\u4f53\u304c\u306a\u3044\u5834\u5408\u306b\u306f\u3001\u6297\u4f53\u3092\u81ea\u5bb6\u6a19\u8b58\u3057\u3066\u7528\u3044\u308b\u3053\u3068\u3082\u3042\u308a\u307e\u3059\u3002<strong>\u86cd\u5149\u6a19\u8b58\u3067\u306f\u672a\u53cd\u5fdc\u306e\u904a\u96e2\u86cd\u5149\u8272\u7d20\u306b\u3088\u308b\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9\u30ce\u30a4\u30ba\u306e\u4e0a\u6607\u306f\u3088\u304f\u77e5\u3089\u308c\u3066\u3044\u307e\u3059<\/strong>\u3002\u4e00\u822c\u306b\u86cd\u5149\u8272\u7d20\u306f\u4f1a\u5408\u3057\u3084\u3059\u304f\u3001\u900f\u6790\u3084\u30b2\u30eb\u6ffe\u904e\u3067\u306f\u9664\u53bb\u304c\u96e3\u3057\u3044\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u5f0a\u793e\u306e\u86cd\u5149\u6a19\u8b58\u30ad\u30c3\u30c8\u3067\u306f\u72ec\u81ea\u306e\u7cbe\u88fd\u30ec\u30b8\u30f3\u3092\u5145\u586b\u3057\u305f\u30b9\u30d4\u30f3\u30ab\u30e9\u30e0\u306b\u3088\u308b\u3001\u672a\u53cd\u5fdc\u306e\u904a\u96e2\u86cd\u5149\u8272\u7d20\u306e\u52b9\u679c\u7684\u306a\u9664\u53bb\u304c\u53ef\u80fd\u3067\u3059\u3002<\/p>\n<h3>\u6297\u4f53\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u306e\u5229\u7528<\/h3>\n<p>\u6297\u4f53\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u3068\u306f\u9175\u7d20\u6d88\u5316\u306b\u3088\u308a\u5b9a\u5e38\u90e8\u4f4d(Fc)\u3092\u9664\u53bb\u3057\u305f\u6297\u4f53\u306e\u65ad\u7247(\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8)\u3067\u30011\u4fa1\u306eFab\u3084Fab\u304c\u30d2\u30f3\u30b8\u90e8\u4f4d\u3067\u7d50\u5408\u3057\u305f2\u4fa1\u306eF(ab\u2019)<sub>2<\/sub>\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<div id=\"attachment_1077\" style=\"width: 510px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/Immunohistochemistry2-fig1.jpg\" rel=\"attachment wp-att-1077\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-1077\" class=\"size-full wp-image-1077\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/Immunohistochemistry2-fig1.jpg\" alt=\"Immunohistochemistry2-fig1\" width=\"500\" height=\"123\" \/><\/a><p id=\"caption-attachment-1077\" class=\"wp-caption-text\">\u56f31 Fab\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u306e\u8abf\u88fd<\/p><\/div>\n<div id=\"attachment_1078\" style=\"width: 510px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/Immunohistochemistry2-fig2.jpg\" rel=\"attachment wp-att-1078\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-1078\" class=\"size-full wp-image-1078\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/Immunohistochemistry2-fig2.jpg\" alt=\"Immunohistochemistry2-fig2\" width=\"500\" height=\"122\" \/><\/a><p id=\"caption-attachment-1078\" class=\"wp-caption-text\">\u56f32 F(ab\u2019)<sub>2<\/sub>\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u306e\u8abf\u88fd<\/p><\/div>\n<p>\u3053\u306e\u3088\u3046\u306a\u6297\u4f53\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u306fIgG (Whole Molecule)\u306b\u6bd4\u3079\u3066\u3001<strong>\u5206\u5b50\u91cf\u304c\u5c0f\u3055\u304f\u7d44\u7e54\u3078\u306e\u6d78\u900f\u304c\u6bd4\u8f03\u7684\u3088\u3044<\/strong>\u306e\u304c\u7279\u9577\u3067\u3059\u3002\u307e\u305f\u3001\u6297Fab\u3084\u6297F(ab\u2019)<sub>2<\/sub>\u6297\u4f53\u30922\u6b21\u6297\u4f53\u3068\u3057\u3066\u7528\u3044\u308b\u3053\u3068\u3067\u3001\u5185\u5728\u3059\u308bIgG\u3068\u306e\u53cd\u5fdc(Fc\u3078\u306e\u7d50\u5408)\u3092\u6291\u3048\u3001<strong>\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9\u30ce\u30a4\u30ba\u3092\u8efd\u6e1b\u3067\u304d\u308b<\/strong>\u3053\u3068\u3082\u3042\u308a\u307e\u3059\u3002<\/p>\n<h2>\u53c2\u8003\u6587\u732e<\/h2>\n<ol>\n<li>Hsu, S.M., et al. (1981). Am. J. Clin. Path. 75, 816.<\/li>\n<li>Hsu, S.M., et al. (1981). J. Histochem. Cytochem. 29, 577-580.<\/li>\n<li>Hsu, S.M., et al. (1981). Am. J. Clin. Path. 75, 734-738.<\/li>\n<li>Guesdon, J.L., et al. (1983). J. Histochem. Cytochem. 27, 1131.<\/li>\n<\/ol>\n<h1>\u3010\u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3011\u30bf\u30f3\u30d1\u30af\u8cea\u89e3\u6790\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u30cf\u30f3\u30c9\u30d6\u30c3\u30af<\/h1>\n<p>\u52b9\u7387\u7684\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u62bd\u51fa\u304b\u3089\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u89e3\u6790\u30c4\u30fc\u30eb\u307e\u3067\u3001\u5305\u62ec\u7684\u306b\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u3092\u7d39\u4ecb\u3057\u3066\u304a\u308a\u307e\u3059\u3002PDF\u30d5\u30a1\u30a4\u30eb\u306e\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3092\u3054\u5e0c\u671b\u306e\u65b9\u306f\u3001\u4e0b\u8a18\u30dc\u30bf\u30f3\u3088\u308a\u304a\u7533\u8fbc\u307f\u304f\u3060\u3055\u3044\u3002<\/p>\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/catalogs-protein-form.html?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa49_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/p>\n<h2>\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u691c\u51fa\u30fb\u89e3\u6790\u95a2\u9023\u3001\u305d\u306e\u4ed6\u306e\u8a18\u4e8b\u306f\u3053\u3061\u3089<\/h2>\n<table style=\"border-color: lightgray;border-collapse: collapse;padding: 20px\" border=\"1\">\n<tbody>\n<tr>\n<td style=\"border-color: lightgray;padding: 20px\">\n<ul>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic10\/?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa49_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u7b2c10\u56de\u301110\u5206\u3067\u5206\u304b\u308b\u30d3\u30aa\u30c1\u30f3\u7d50\u5408\u30bf\u30f3\u30d1\u30af\u8cea\u307e\u3068\u3081\n<p><\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic11\/?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa49_Social_LAB\" target=\"_blank\" 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