{"id":350,"date":"2016-04-07T00:00:00","date_gmt":"2016-04-06T15:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/digitalpcr3\/"},"modified":"2025-08-25T15:30:15","modified_gmt":"2025-08-25T06:30:15","slug":"digitalpcr3","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/digitalpcr3\/","title":{"rendered":"\u30c7\u30b8\u30bf\u30ebPCR\u306b\u3088\u308b\u9ad8\u7cbe\u5ea6\u306a\u30b3\u30d4\u30fc\u6570\u591a\u578b (CNV) \u89e3\u6790"},"content":{"rendered":"\n<div class=\"wp-block-group is-vertical is-layout-flex wp-container-core-group-is-layout-8cf370e7 wp-block-group-is-layout-flex\">\n<p class=\"has-small-font-size\">*\u66f4\u65b0\u65e5\uff1a2025.08.25<\/p>\n\n\n\n<p>\u30b3\u30d4\u30fc\u6570\u591a\u578b\u89e3\u6790\uff08Copy Number Variation\uff1aCNV\uff09\u306f\u30b2\u30ce\u30e0\u306b\u751f\u3058\u305f\u5909\u7570\u3067\u3001\u30b2\u30ce\u30e0DNA\u914d\u5217\u306e\u30b3\u30d4\u30fc\u6570\u304c\u3001\u30ea\u30d5\u30a1\u30ec\u30f3\u30b9\u3084\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\u3068\u7570\u306a\u308b\u3082\u306e\u3092\u6307\u3057\u307e\u3059\uff08\u56f31\uff09\u3002\u30b3\u30d4\u30fc\u6570\u304c\u5897\u3048\u308bAmplification\u3068\u3001\u30b3\u30d4\u30fc\u6570\u304c\u6e1b\u308bDeletion\u304c\u3042\u308a\u307e\u3059\u3002\u633f\u5165\u3001\u6b20\u5931\u3001\u9006\u4f4d\u3001\u8ee2\u5ea7\u3068\u3044\u3063\u305f\u30b2\u30ce\u30e0\u4e0a\u306e\u5909\u5316\u304cCNV\u3092\u5f15\u304d\u8d77\u3053\u3057\u307e\u3059\u3002CNV\u306f\u30d2\u30c8\u30b2\u30ce\u30e0\u3067\u3082\u3088\u304f\u898b\u3089\u308c\u308b\u907a\u4f1d\u5b50\u5909\u7570\u306e\u4e00\u3064\u3067\u3001\u304c\u3093\u3084\u907a\u4f1d\u6027\u75be\u60a3\u3092\u542b\u3081\u3001\u6570\u591a\u304f\u306e\u75be\u60a3\u306b\u95a2\u4fc2\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-group is-vertical is-content-justification-center is-layout-flex wp-container-core-group-is-layout-4b2eccd6 wp-block-group-is-layout-flex\">\n<figure class=\"wp-block-image aligncenter size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"396\" height=\"220\" src=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/08\/4269e962bb19106a958c518abcc0d034.png\" alt=\"\" class=\"wp-image-22825\" style=\"width:510px\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/08\/4269e962bb19106a958c518abcc0d034.png 396w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/08\/4269e962bb19106a958c518abcc0d034-300x167.png 300w\" sizes=\"auto, (max-width: 396px) 100vw, 396px\" \/><\/figure>\n\n\n\n<p class=\"has-text-align-center\"><strong>\u56f31. CNV\u306e\u30a4\u30e1\u30fc\u30b8\u56f3<\/strong><\/p>\n<\/div>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>\u30c7\u30b8\u30bf\u30ebPCR\u306fCNV\u89e3\u6790\u306b\u3082\u6709\u7528\u306a\u624b\u6bb5\u3067\u3059\u3002\u672c\u8a18\u4e8b\u3067\u306f\u5177\u4f53\u4f8b\u3092\u4ea4\u3048\u306a\u304c\u3089\u3001\u30c7\u30b8\u30bf\u30ebPCR\u3067\u306eCNV\u89e3\u6790\u306b\u3064\u3044\u3066\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n\n\n\n<div class=\"wp-block-group is-vertical is-layout-flex wp-container-core-group-is-layout-8cf370e7 wp-block-group-is-layout-flex\">\n<p><strong>\u25bc\u3053\u306e\u3088\u3046\u306a\u65b9\u306b\u304a\u3059\u3059\u3081<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u30c7\u30b8\u30bf\u30ebPCR\u306b\u3054\u8208\u5473\u306e\u3042\u308b\u65b9<\/strong><\/li>\n\n\n\n<li><strong>\u304c\u3093\u3084\u75be\u60a3\u95a2\u9023\u306e\u7814\u7a76\u3092\u3055\u308c\u3066\u3044\u308b\u65b9<\/strong><\/li>\n\n\n\n<li><strong>CNV\u89e3\u6790\u3092\u5b9f\u65bd\u3057\u3066\u3044\u308b\u65b9<\/strong><\/li>\n<\/ul>\n<\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-cnv\u89e3\u6790\u306e\u624b\u6cd5\">CNV\u89e3\u6790\u306e\u624b\u6cd5<\/h2>\n\n\n\n<p>CNV\u691c\u51fa\u306e\u65b9\u6cd5\u3068\u3057\u3066\u306f\u3001\u6bd4\u8f03\u30b2\u30ce\u30e0\u30cf\u30a4\u30d6\u30ea\u30c0\u30a4\u30bc\u30fc\u30b7\u30e7\u30f3\uff08CGH\uff09\u3001\u30a2\u30ec\u30a4\u6bd4\u8f03\u30b2\u30ce\u30e0\u30cf\u30a4\u30d6\u30ea\u30c0\u30a4\u30bc\u30fc\u30b7\u30e7\u30f3\uff08aCGH\uff09\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\uff08qPCR\uff09\u3001\u6b21\u4e16\u4ee3\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\uff08NGS\uff09\u3001\u30c7\u30b8\u30bf\u30ebPCR\u306a\u3069\u3055\u307e\u3056\u307e\u306a\u65b9\u6cd5\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n\n\n\n<div class=\"wp-block-group is-vertical is-layout-flex wp-container-core-group-is-layout-8cf370e7 wp-block-group-is-layout-flex\">\n<p>\u88681. \u3055\u307e\u3056\u307e\u306aCNV\u691c\u51fa\u6cd5<\/p>\n\n\n\n<figure class=\"wp-block-table\">\n  <table class=\"has-fixed-layout\">\n    <colgroup>\n      <col style=\"width: 20%\">\n      <col style=\"width: 15%\">\n      <col style=\"width: 25%\"> <!-- \u4e2d\u592e\u306e\u5217\u306e\u5e45\u3092\u72ed\u304f -->\n      <col style=\"width: 25%\"> <!-- \u4ed6\u306e\u5217\u306e\u5e45\u3092\u8abf\u6574 -->\n      <col style=\"width: 15%\">\n    <\/colgroup>\n    <tbody>\n      <tr>\n        <td><\/td>\n        <td>aCGH<\/td>\n        <td>\u6b21\u4e16\u4ee3\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0<\/td>\n        <td>\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR<\/td>\n        <td>\u30c7\u30b8\u30bf\u30ebPCR<\/td>\n      <\/tr>\n      <tr>\n        <td>\u30cf\u30f3\u30ba\u30aa\u30f3\u30bf\u30a4\u30e0<\/td>\n        <td>\u9577\u3044<\/td>\n        <td>\u9577\u3044<\/td>\n        <td>\u77ed\u3044<\/td>\n        <td>\u77ed\u3044<\/td>\n      <\/tr>\n      <tr>\n        <td>\u6240\u8981\u6642\u9593<\/td>\n        <td>\u6570\u65e5<\/td>\n        <td>\u6570\u65e5<\/td>\n        <td>\u6570\u6642\u9593<\/td>\n        <td>\u6570\u6642\u9593<\/td>\n      <\/tr>\n      <tr>\n        <td>\u5b9a\u91cf\u306e\u7cbe\u5ea6<\/td>\n        <td>\u2605<\/td>\n        <td>\u2605\u2605<\/td>\n        <td>\u2605\u2605<\/td>\n        <td>\u2605\u2605\u2605<\/td>\n      <\/tr>\n      <tr>\n        <td>\u89e3\u50cf\u5ea6<\/td>\n        <td>\u2605\u2605<\/td>\n        <td>\u2605\u2605\u2605<\/td>\n        <td>\u2605\u2605\u2605<\/td>\n        <td>\u2605\u2605\u2605<\/td>\n      <\/tr>\n    <\/tbody>\n  <\/table>\n<\/figure>\n<\/div>\n\n\n\n<p>\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u30d9\u30fc\u30b9\u306eaCGH\u306f\u30cf\u30f3\u30ba\u30aa\u30f3\u30bf\u30a4\u30e0\u304c\u9577\u304f\u3001\u6240\u8981\u6642\u9593\u3082\u9577\u304f\u306a\u308a\u307e\u3059\u3002\u6b21\u4e16\u4ee3\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\u306f\u7db2\u7f85\u7684\u306a\u89e3\u6790\u304c\u3067\u304d\u308b\u305f\u3081\u3001\u591a\u304f\u306eCNV\u306b\u3064\u3044\u3066\u4e00\u5ea6\u306b\u8abf\u3079\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u4e00\u65b9\u3001\u30b3\u30b9\u30c8\u304c\u9ad8\u304f\u6240\u8981\u6642\u9593\u3082\u9577\u304f\u306a\u308a\u307e\u3059\u3002\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3084\u30c7\u30b8\u30bf\u30ebPCR\u306f\u7279\u5b9a\u306eCNV\u3092\u5b89\u4fa1\u3067\u77ed\u6642\u9593\u306b\u6e2c\u5b9a\u3067\u304d\u308b\u305f\u3081\u3001\u8a55\u4fa1\u3057\u305f\u3044CNV\u304c\u7d5e\u3089\u308c\u3066\u3044\u308b\u5834\u5408\u306b\u6709\u7528\u3067\u3059\u3002<\/p>\n\n\n\n<p>\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3068\u30c7\u30b8\u30bf\u30ebPCR\u3092\u6bd4\u8f03\u3059\u308b\u3068\u3001\u7279\u306b\u30b3\u30d4\u30fc\u6570\u304c\u591a\u3044\u5834\u5408\u306f\u30c7\u30b8\u30bf\u30ebPCR\u304c\u304a\u3059\u3059\u3081\u3067\u3059\u3002\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306fCt\u5024\u306e\u5dee\u304b\u3089\u5b9a\u91cf\u3092\u884c\u3044\u307e\u3059\u304c\u3001\u30c7\u30b8\u30bf\u30ebPCR\u306f\u7d76\u5bfe\u5b9a\u91cf\u3092\u884c\u3046\u88c5\u7f6e\u3067\u3042\u308b\u305f\u3081\u3001\u308f\u305a\u304b\u306a\u5dee\u3082\u691c\u51fa\u3067\u304d\u307e\u3059\u3002\u4f8b\u3048\u30707\u30b3\u30d4\u30fc\u30688\u30b3\u30d4\u30fc\u306f1.14\u500d\u3057\u304b\u5dee\u304c\u3042\u308a\u307e\u305b\u3093\u304c\u3001\u30c7\u30b8\u30bf\u30ebPCR\u306b\u3088\u308b\u6e2c\u5b9a\u3067\u306f\u7d71\u8a08\u5b66\u7684\u306b\u512a\u4f4d\u306a\u5dee\u304c\u898b\u3089\u308c\u3066\u3044\u307e\u3059\uff08\u56f32\uff09\u3002\u4e00\u65b9\u3067\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3067\u306f7\u30b3\u30d4\u30fc\u30688\u30b3\u30d4\u30fc\u306e\u5dee\u306fCt\u5024\u3067\u308f\u305a\u304b0.2\u7a0b\u5ea6\u306e\u5dee\u3068\u306a\u308a\u3001\u7cbe\u5ea6\u306e\u9ad8\u3044\u6e2c\u5b9a\u306f\u96e3\u3057\u3044\u3067\u3059\u3002<\/p>\n\n\n\n<div class=\"wp-block-group is-vertical is-content-justification-center is-layout-flex wp-container-core-group-is-layout-4b2eccd6 wp-block-group-is-layout-flex\">\n<figure class=\"wp-block-image aligncenter size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"921\" height=\"541\" src=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/08\/8f413a46f9c58d650436a78bab3c0837.png\" alt=\"\" class=\"wp-image-22826\" style=\"width:669px;height:auto\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/08\/8f413a46f9c58d650436a78bab3c0837.png 921w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/08\/8f413a46f9c58d650436a78bab3c0837-300x176.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/08\/8f413a46f9c58d650436a78bab3c0837-768x451.png 768w\" sizes=\"auto, (max-width: 921px) 100vw, 921px\" \/><\/figure>\n\n\n\n<p class=\"has-text-align-center\"><strong>\u56f32. \u30c7\u30b8\u30bf\u30ebPCR\u3067\u6e2c\u5b9a\u3057\u305fCNV\u306e\u30c7\u30fc\u30bf\u4f8b<\/strong><\/p>\n<\/div>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>\u307e\u305f\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3067\u30b3\u30d4\u30fc\u6570\u3092\u6b63\u78ba\u306b\u77e5\u308b\u305f\u3081\u306b\u306f\u3001\u30b3\u30d4\u30fc\u6570\u304c\u5206\u304b\u3063\u3066\u3044\u308b\u30ea\u30d5\u30a1\u30ec\u30f3\u30b9\u30b5\u30f3\u30d7\u30eb\u304c\u5fc5\u8981\u3067\u3059\u3002\u30ea\u30d5\u30a1\u30ec\u30f3\u30b9\u30b5\u30f3\u30d7\u30eb\u3068Ct\u5024\u3092\u6bd4\u8f03\u3059\u308b\u3053\u3068\u3067\u672a\u77e5\u30b5\u30f3\u30d7\u30eb\u306e\u30b3\u30d4\u30fc\u6570\u3092\u6c7a\u5b9a\u3059\u308b\u305f\u3081\u3067\u3059\u3002\u4e00\u65b9\u3067\u30c7\u30b8\u30bf\u30ebPCR\u306f\u7d76\u5bfe\u5b9a\u91cf\u3092\u884c\u3046\u88c5\u7f6e\u3067\u3042\u308b\u305f\u3081\u3001\u30ea\u30d5\u30a1\u30ec\u30f3\u30b9\u30b5\u30f3\u30d7\u30eb\u3092\u6e2c\u5b9a\u3057\u306a\u304f\u3066\u3082\u30b3\u30d4\u30fc\u6570\u3092\u6c42\u3081\u3089\u308c\u307e\u3059\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u30c7\u30b8\u30bf\u30ebpcr\u3067\u306ecnv\u89e3\u6790\u65b9\u6cd5\">\u30c7\u30b8\u30bf\u30ebPCR\u3067\u306eCNV\u89e3\u6790\u65b9\u6cd5<\/h2>\n\n\n\n<p>CNV\u89e3\u6790\u3092\u884c\u3046\u969b\u306f\u6e2c\u5b9a\u3057\u305f\u3044\u907a\u4f1d\u5b50\u3060\u3051\u3067\u306a\u304f\u3001\u30ea\u30d5\u30a1\u30ec\u30f3\u30b9\u9818\u57df\u306e\u6e2c\u5b9a\u3082\u5fc5\u8981\u3067\u3059\u3002\u30b5\u30f3\u30d7\u30eb\u6dfb\u52a0\u91cf\u306b\u3088\u3063\u3066\u92f3\u578b\u91cf\u306f\u5909\u52d5\u3059\u308b\u305f\u3081\u3001\u30ea\u30d5\u30a1\u30ec\u30f3\u30b91\u30b3\u30d4\u30fc\u306b\u5bfe\u3057\u3066\u4f55\u30b3\u30d4\u30fc\u3042\u308b\u304b\u3068\u3044\u3046\u5024\u3092\u7528\u3044\u3066\u30b5\u30f3\u30d7\u30eb\u9593\u306e\u6bd4\u8f03\u3092\u884c\u3044\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u30ea\u30d5\u30a1\u30ec\u30f3\u30b9\u306f\u30d2\u30c8\u30b5\u30f3\u30d7\u30eb\u306e\u5834\u5408\u306b\u306fRNase P\u304c\u4f7f\u7528\u3055\u308c\u308b\u3053\u3068\u304c\u591a\u3044\u3067\u3059\u30021\u7d30\u80de\u3042\u305f\u308a2\u30b3\u30d4\u30fc\uff081\u30b2\u30ce\u30e0\u3042\u305f\u308a1\u30b3\u30d4\u30fc\uff09\u3067\u5b89\u5b9a\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u3044\u308b\u907a\u4f1d\u5b50\u3067\u3059\u3002RNase P\u3068\u30b3\u30d4\u30fc\u6570\u3092\u6bd4\u8f03\u3059\u308b\u3053\u3068\u3067\u30011\u30b2\u30ce\u30e0\u3042\u305f\u308a\u306e\u30b3\u30d4\u30fc\u6570\u3092\u77e5\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\uff08\u56f33\uff09\u3002<\/p>\n\n\n\n<div class=\"wp-block-group is-vertical is-content-justification-center is-layout-flex wp-container-core-group-is-layout-4b2eccd6 wp-block-group-is-layout-flex\">\n<figure class=\"wp-block-image aligncenter size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"411\" height=\"201\" src=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/08\/0d9f3b25cbd469f5dd3d8141e1597f18.png\" alt=\"\" class=\"wp-image-22827\" style=\"width:669px\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/08\/0d9f3b25cbd469f5dd3d8141e1597f18.png 411w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/08\/0d9f3b25cbd469f5dd3d8141e1597f18-300x147.png 300w\" sizes=\"auto, (max-width: 411px) 100vw, 411px\" \/><\/figure>\n\n\n\n<p class=\"has-text-align-center\"><strong>\u56f33. \u30ea\u30d5\u30a1\u30ec\u30f3\u30b9\u3092\u7528\u3044\u305f\u30b3\u30d4\u30fc\u6570\u306e\u7b97\u51fa<\/strong><\/p>\n<\/div>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>\u307e\u305f\u5236\u9650\u9175\u7d20\u51e6\u7406\u304c\u5fc5\u8981\u306a\u4e8b\u4f8b\u3082\u3042\u308a\u307e\u3059\u3002\u8fd1\u508d\u3067\u5897\u52a0\u3057\u3066\u3044\u308b\u5834\u5408\u3001\u30de\u30a4\u30af\u30ed\u30c1\u30e3\u30f3\u30d0\u30fc\u3078\u3064\u306a\u304c\u3063\u305f\u72b6\u614b\u3067\u5206\u914d\u3055\u308c\u308b\u305f\u3081\u3001\u30b3\u30d4\u30fc\u6570\u304c\u904e\u5c0f\u8a55\u4fa1\u3055\u308c\u3066\u3057\u307e\u3044\u307e\u3059\u3002\u5236\u9650\u9175\u7d20\u51e6\u7406\u3092\u3059\u308b\u3053\u3068\u3067\u30de\u30a4\u30af\u30ed\u30c1\u30e3\u30f3\u30d0\u30fc\u3078\u72ec\u7acb\u3057\u3066\u5206\u914d\u3055\u308c\u308b\u3088\u3046\u306b\u306a\u308b\u305f\u3081\u3001\u9069\u5207\u306a\u30b3\u30d4\u30fc\u6570\u3092\u8a55\u4fa1\u3067\u304d\u308b\u3088\u3046\u306b\u306a\u308a\u307e\u3059\uff08\u56f34\uff09\u3002<\/p>\n\n\n\n<div class=\"wp-block-group is-vertical is-content-justification-center is-layout-flex wp-container-core-group-is-layout-4b2eccd6 wp-block-group-is-layout-flex\">\n<figure class=\"wp-block-image aligncenter size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"969\" height=\"497\" src=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/08\/b38903a7198fa10d382ef483e2dbdc88.png\" alt=\"\u56f34. \u5236\u9650\u9175\u7d20\u51e6\u7406\u306e\u30a4\u30e1\u30fc\u30b8\u56f3\" class=\"wp-image-22829\" style=\"width:669px\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/08\/b38903a7198fa10d382ef483e2dbdc88.png 969w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/08\/b38903a7198fa10d382ef483e2dbdc88-300x154.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2025\/08\/b38903a7198fa10d382ef483e2dbdc88-768x394.png 768w\" sizes=\"auto, (max-width: 969px) 100vw, 969px\" \/><\/figure>\n\n\n\n<p class=\"has-text-align-center\"><strong>\u56f34. \u5236\u9650\u9175\u7d20\u51e6\u7406\u306e\u30a4\u30e1\u30fc\u30b8\u56f3<\/strong><\/p>\n<\/div>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u30c7\u30b8\u30bf\u30ebpcr\u3067\u306e\u6e2c\u5b9a\u4e8b\u4f8b\">\u30c7\u30b8\u30bf\u30ebPCR\u3067\u306e\u6e2c\u5b9a\u4e8b\u4f8b<\/h2>\n\n\n\n<p>2020\u5e74\u3001Lingxia Jiang\u3089\u306b\u3088\u3063\u3066\u5831\u544a\u3055\u308c\u305f\u306e\u306f\u3001\u810a\u9ac4\u6027\u7b4b\u840e\u7e2e\u75c7\uff08SMA\uff09\u306b\u95a2\u9023\u3059\u308b\u907a\u4f1d\u5b50\u306eCNV\u89e3\u6790\u4e8b\u4f8b\u3067\u3059\u3002\u3053\u306e\u5b9f\u9a13\u7cfb\u306f4\u3064\u306e\u30bf\u30fc\u30b2\u30c3\u30c8\u3092\u540c\u6642\u306b\u6e2c\u5b9a\u3059\u308b\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u306e\u7cfb\u3067\u3059\u3002\u30ea\u30d5\u30a1\u30ec\u30f3\u30b9\u306f<em>RPPH1<\/em>\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u306f<em>SMN1<\/em>\u3001<em>SMN2<\/em>\u306b\u52a0\u3048\u3066<em>SMN1<\/em>\u3068<em>SMN2<\/em>\u306e\u4e21\u65b9\u3092\u691c\u51fa\u3059\u308b\u30a2\u30c3\u30bb\u30a4\u3092\u4f7f\u7528\u3057\u3066\u305d\u308c\u305e\u308c\u306e\u30b3\u30d4\u30fc\u6570\u3092\u6e2c\u5b9a\u3057\u3066\u3044\u307e\u3059\u3002\u60a3\u8005\u7531\u6765\u306e\u30b5\u30f3\u30d7\u30eb\u3067\u3082\u691c\u8a3c\u304c\u884c\u308f\u308c\u3001\u6b63\u3057\u3044\u7d50\u679c\u304c\u5f97\u3089\u308c\u305f\u3068\u5831\u544a\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u5b9f\u9a13\u7cfb\u3084\u30c7\u30fc\u30bf\u306e\u8a73\u7d30\u306f\u6587\u732e\u3092\u3054\u89a7\u304f\u3060\u3055\u3044<sup>[1]<\/sup>\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u307e\u3068\u3081\">\u307e\u3068\u3081<\/h2>\n\n\n\n<p>CNV\u306e\u691c\u51fa\u306b\u306f\u7cbe\u5ea6\u3068\u6b63\u78ba\u6027\u306e\u4e21\u65b9\u304c\u6c42\u3081\u3089\u308c\u307e\u3059\u3002\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306a\u3069\u306e\u4e00\u822c\u7684\u306b\u3088\u304f\u5229\u7528\u3055\u308c\u3066\u3044\u308b\u5f93\u6765\u306e\u30c6\u30af\u30ce\u30ed\u30b8\u30fc\u306f\u3001\u308f\u305a\u304b\u306a\u30b3\u30d4\u30fc\u6570\u306e\u5dee\u306e\u5224\u5225\u304c\u56f0\u96e3\u3067\u3042\u3063\u305f\u308a\u3001\u30ea\u30d5\u30a1\u30ec\u30f3\u30b9\u30b5\u30f3\u30d7\u30eb\u3084\u6a19\u6e96\u66f2\u7dda\u304c\u5fc5\u8981\u3060\u3063\u305f\u308a\u306a\u3069\u306e\u5236\u7d04\u304c\u3042\u308a\u307e\u3059\u3002\u3053\u308c\u3089\u306e\u5236\u7d04\u306b\u5bfe\u3057\u3066\u6709\u52b9\u306a\u624b\u6cd5\u304c\u30c7\u30b8\u30bf\u30ebPCR\u3067\u3059\u3002\u5f53\u793e\u3067\u306fCNV\u89e3\u6790\u7528\u306e\u8a2d\u8a08\u6e08\u307fTaqMan\u2122 Assay\u3067\u3042\u308bApplied Biosystems\u2122 <a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/pcr\/real-time-pcr\/real-time-pcr-assays\/cnv-analysis-using-taqman.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_oc_s00_qpcr36_Social_LAB\" target=\"_blank\" rel=\"noreferrer noopener\">TaqMan\u2122 Copy Number Assays<\/a>\u3092\u8ca9\u58f2\u3057\u3066\u3044\u307e\u3059\u3002\u30b7\u30f3\u30d7\u30eb\u3067\u304a\u624b\u9803\u306a\u30c7\u30b8\u30bf\u30ebPCR\u30d7\u30e9\u30c3\u30c8\u30d5\u30a9\u30fc\u30e0\u3001Applied Biosystems\u2122 QuantStudio\u2122 Absolute Q\u2122\u30c7\u30b8\u30bf\u30ebPCR\u30b7\u30b9\u30c6\u30e0\u3068\u7d44\u307f\u5408\u308f\u305b\u308b\u3053\u3068\u3067\u7cbe\u5ea6\u306e\u9ad8\u3044CNV\u89e3\u6790\u3092\u53ef\u80fd\u306b\u3057\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u30c7\u30b8\u30bf\u30ebPCR\u306e\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u3092\u307e\u3068\u3081\u305f\u8cc7\u6599\u3092\u7121\u511f\u3067\u914d\u5e03\u3057\u3066\u3044\u307e\u3059\u3002\u30c7\u30b8\u30bf\u30ebPCR\u306e\u6d3b\u7528\u4e8b\u4f8b\u3092\u305c\u3072\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n<h4 class=\"wp-block-heading\" id=\"h-\u30c7\u30b8\u30bf\u30ebpcr\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u30ac\u30a4\u30c9-\u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\">\u30c7\u30b8\u30bf\u30ebPCR\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u30ac\u30a4\u30c9 \u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9<\/h4>\n\n\n\n<div style=\"height:15px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/life-science\/dpcr-applications-ebook-jp.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_oc_s00_qpcr36_Social_LAB\" target=\"_blank\" rel=\"noreferrer noopener\">\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u306f\u3053\u3061\u3089<\/a><\/p>\n\n\n\n<div class=\"wp-block-group is-vertical is-layout-flex wp-container-core-group-is-layout-8cf370e7 wp-block-group-is-layout-flex\">\n<p>Reference:<\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li>Jiang, L., Lin, R., Gallagher, S. et al. Development and validation of a 4-color multiplexing spinal muscular atrophy \uff08SMA\uff09 genotyping assay on a novel integrated digital PCR instrument. Sci Rep 10, 19892 \uff082020\uff09. https:\/\/doi.org\/10.1038\/s41598-020-76893-7<\/li>\n<\/ol>\n<\/div>\n\n\n\n<div class=\"wp-block-group is-layout-constrained wp-block-group-is-layout-constrained\">\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u7528\u306b\u306f\u4f7f\u7528\u3044\u305f\u3060\u3051\u307e\u305b\u3093\u3002<\/p>\n<\/div>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u30c7\u30b8\u30bf\u30ebPCR (dPCR)\u3092\u3054\u7d39\u4ecb\u3059\u308b\u30016\u56de\u30b7\u30ea\u30fc\u30ba\u306e\u30d1\u30fc\u30c83\u3067\u3059\u3002\u4eca\u56de\u306f\u3001\u30c7\u30b8\u30bf\u30ebPCR\u304c\u3069\u306e\u3088\u3046\u306b\u9ad8\u7cbe\u5ea6\u306a\u30b3\u30d4\u30fc\u6570\u591a\u578b(CNV)\u89e3\u6790\u3092\u53ef\u80fd\u306b\u3057\u3066\u3044\u308b\u304b\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n","protected":false},"author":988,"featured_media":2558,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[156],"tags":[44],"division":[],"class_list":{"0":"post-350","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-mol-bio","8":"tag-digital-pcr","9":"entry"},"_selected_authors":[],"_selected_reviewers":[],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.8 (Yoast SEO v27.8) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>\u30c7\u30b8\u30bf\u30ebPCR\u306b\u3088\u308b\u9ad8\u7cbe\u5ea6\u306a\u30b3\u30d4\u30fc\u6570\u591a\u578b (CNV) \u89e3\u6790 - Learning at the Bench<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/digitalpcr3\/\" \/>\n<meta property=\"og:locale\" content=\"ja_JP\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"\u30c7\u30b8\u30bf\u30ebPCR\u306b\u3088\u308b\u9ad8\u7cbe\u5ea6\u306a\u30b3\u30d4\u30fc\u6570\u591a\u578b (CNV) \u89e3\u6790\" \/>\n<meta property=\"og:description\" content=\"\u30c7\u30b8\u30bf\u30ebPCR (dPCR)\u3092\u3054\u7d39\u4ecb\u3059\u308b\u30016\u56de\u30b7\u30ea\u30fc\u30ba\u306e\u30d1\u30fc\u30c83\u3067\u3059\u3002\u4eca\u56de\u306f\u3001\u30c7\u30b8\u30bf\u30ebPCR\u304c\u3069\u306e\u3088\u3046\u306b\u9ad8\u7cbe\u5ea6\u306a\u30b3\u30d4\u30fc\u6570\u591a\u578b(CNV)\u89e3\u6790\u3092\u53ef\u80fd\u306b\u3057\u3066\u3044\u308b\u304b\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/digitalpcr3\/\" \/>\n<meta property=\"og:site_name\" content=\"Learning at the Bench\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/ThermoFisherJapan\" \/>\n<meta property=\"article:published_time\" content=\"2016-04-06T15:00:00+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2025-08-25T06:30:15+00:00\" \/>\n<meta property=\"og:image\" content=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/digitalpcr-title-2.jpeg\" \/>\n\t<meta property=\"og:image:width\" content=\"600\" \/>\n\t<meta property=\"og:image:height\" content=\"315\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"LATB Staff\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@ThermoFisherJP\" \/>\n<meta name=\"twitter:site\" content=\"@ThermoFisherJP\" \/>\n<meta name=\"twitter:label1\" content=\"\u57f7\u7b46\u8005\" \/>\n\t<meta name=\"twitter:data1\" content=\"LATB Staff\" \/>\n\t<meta name=\"twitter:label2\" content=\"\u63a8\u5b9a\u8aad\u307f\u53d6\u308a\u6642\u9593\" \/>\n\t<meta name=\"twitter:data2\" content=\"8\u5206\" \/>\n<script type=\"application\/ld+json\" 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