{"id":358,"date":"2016-04-14T00:00:00","date_gmt":"2016-04-13T15:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/digitalpcr4\/"},"modified":"2025-07-01T16:56:11","modified_gmt":"2025-07-01T07:56:11","slug":"digitalpcr4","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/digitalpcr4\/","title":{"rendered":"\u30c7\u30b8\u30bf\u30ebPCR\u306b\u3088\u308b\u6b21\u4e16\u4ee3\u30b7\u30fc\u30b1\u30f3\u30b5(NGS)\u306e\u30e9\u30a4\u30d6\u30e9\u30ea\u306e\u9ad8\u7cbe\u5ea6\u306a\u5b9a\u91cf"},"content":{"rendered":"

\u30b7\u30f3\u30d7\u30eb\u3001\u6b63\u78ba\u3001\u304a\u624b\u9803\u306a\u30c7\u30b8\u30bf\u30ebPCR\u306b\u3088\u308bNGS\u30e9\u30a4\u30d6\u30e9\u30ea\u5b9a\u91cf<\/h2>\n

\u30c7\u30b8\u30bf\u30ebPCR\u306f\u3001NGS\u30e9\u30a4\u30d6\u30e9\u30ea\u5b9a\u91cf<\/a>\u3092\u30b7\u30f3\u30d7\u30eb\u304b\u3064\u6b63\u78ba\u306b\u884c\u3044\u3001\u30de\u30a4\u30af\u30ed\u30c1\u30c3\u30d7\u578b\u96fb\u6c17\u6cf3\u52d5\u3084\u5206\u5149\u5149\u5ea6\u8a08\u3068\u3044\u3063\u305f\u4ed6\u306e\u624b\u6cd5\u3092\u7528\u3044\u305f\u3068\u304d\u306b\u8d77\u3053\u308a\u304c\u3061\u306a\u3001\u30e9\u30a4\u30d6\u30e9\u30ea\u6fc3\u5ea6\u3092\u8aa4\u3063\u3066\u8a55\u4fa1\u3059\u308b\u53ef\u80fd\u6027\u3092\u8efd\u6e1b\u3057\u307e\u3059<\/strong>\u3002\u30c7\u30b8\u30bf\u30ebPCR\u306f\u3001\u3042\u308b\u30e9\u30a4\u30d6\u30e9\u30ea\u306b\u304a\u3044\u3066\u3001\u30d5\u30a9\u30ef\u30fc\u30c9\u3068\u30ea\u30d0\u30fc\u30b9\u30a2\u30c0\u30d7\u30bf\u30fc\u306e\u4e21\u65b9\u3092\u30ab\u30d0\u30fc\u3059\u308b\u3088\u3046\u306b\u30c7\u30b6\u30a4\u30f3\u3055\u308c\u305f\u7279\u7570\u6027\u306e\u9ad8\u3044\u30a2\u30c3\u30bb\u30a4\u3092\u4f7f\u7528\u3057\u3001\u4e21\u65b9\u306e\u30e9\u30a4\u30d6\u30e9\u30ea\u30a2\u30c0\u30d7\u30bf\u30fc\u914d\u5217\u3092\u542b\u3080\u69cb\u9020\u306e\u307f\u3092\u691c\u51fa\u3057\u307e\u3059\u3002<\/p>\n

\"digitalpcr4-fig1\"<\/a>

\u56f31 NGS\u30e9\u30a4\u30d6\u30e9\u30ea\u5b9a\u91cf\u306b\u4f7f\u7528\u3059\u308b\u30a2\u30c3\u30bb\u30a4\u30c7\u30b6\u30a4\u30f3\u3002\u30d5\u30a9\u30ef\u30fc\u30c9\u30d7\u30e9\u30a4\u30de\u30fc\u3001\u30ea\u30d0\u30fc\u30b9\u30d7\u30e9\u30a4\u30de\u30fc\u3001\u30d7\u30ed\u30fc\u30d6\u3067\u69cb\u6210\u3002<\/p><\/div>\n

\u30c7\u30b8\u30bf\u30ebPCR\u306f\u6a19\u6e96\u66f2\u7dda\u3084\u30ea\u30d5\u30a1\u30ec\u30f3\u30b9\u30b5\u30f3\u30d7\u30eb\u3092\u4f7f\u7528\u305b\u305a\u3001NGS\u30e9\u30a4\u30d6\u30e9\u30ea\u3092\u6b63\u78ba\u306b\u5b9a\u91cf\u3067\u304d\u308b\u306e\u3067\u3001\u5b9f\u9a13\u306e\u624b\u9593\u3084\u5931\u6557\u306e\u30ea\u30b9\u30af\u3092\u6700\u5c0f\u9650\u306b\u6291\u3048\u3001\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u306b\u304b\u304b\u308b\u30b3\u30b9\u30c8\u3092\u8efd\u6e1b\u3067\u304d\u307e\u3059\u3002<\/p>\n

\u307e\u305f\u3001\u30c7\u30b8\u30bf\u30ebPCR\u306f\u3001\u30b7\u30f3\u30d7\u30eb\u306a\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u306b\u3088\u308a\u3001\u308f\u305a\u304b\u6570\u6642\u9593\u3067\u30e9\u30a4\u30d6\u30e9\u30ea\u6fc3\u5ea6\u3092\u7cbe\u5bc6\u306b\u6e2c\u5b9a\u3067\u304d\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u3001\u30e9\u30f3\u30cb\u30f3\u30b0\u306e\u3084\u308a\u76f4\u3057\u3084\u30b5\u30f3\u30d7\u30eb\u8abf\u88fd\u306e\u5931\u6557\u306e\u30ea\u30b9\u30af\u3092\u6700\u5c0f\u9650\u306b\u6291\u3048\u307e\u3059\u3002NGS\u30e9\u30a4\u30d6\u30e9\u30ea\u306e\u6fc3\u5ea6\u306f\u3001\u9ad8\u3059\u304e\u3066\u3082\u4f4e\u3059\u304e\u3066\u3082\u3001\u30c8\u30fc\u30bf\u30eb\u306e\u30ea\u30fc\u30c9\u6570\u306e\u6e1b\u5c11\u3001\u30c7\u30fc\u30bf\u53ce\u7387\u3084\u30b9\u30eb\u30fc\u30d7\u30c3\u30c8\u306e\u4f4e\u4e0b\u306b\u3064\u306a\u304c\u308a\u307e\u3059<\/strong>\u3002<\/p>\n

\u3069\u306e\u3088\u3046\u306a\u3068\u304d\u306b\u30c7\u30b8\u30bf\u30ebPCR\u3092NGS\u30e9\u30a4\u30d6\u30e9\u30ea\u306e\u5b9a\u91cf\u306b\u4f7f\u7528\u3059\u308b\u304b<\/h2>\n

\u6a19\u6e96\u7684\u306a\u30e9\u30a4\u30d6\u30e9\u30ea\u306b\u306f\u5897\u5e45\u30b9\u30c6\u30c3\u30d7\u304c\u5fc5\u8981\u3067\u3059\u3002\u3053\u308c\u306b\u3088\u308a\u6b63\u3057\u304f\u4f5c\u88fd\u3055\u308c\u305f\u30e9\u30a4\u30d6\u30e9\u30ea(A\u304a\u3088\u3073B\u30a2\u30c0\u30d7\u30bf\u30fc\u306e\u4e21\u65b9\u306e\u914d\u5217\u3092\u542b\u3080\u30e9\u30a4\u30d6\u30e9\u30ea)\u304c\u6fc3\u7e2e\u3055\u308c\u307e\u3059\u3002\u5897\u5e45\u30b9\u30c6\u30c3\u30d7\u306f\u3001\u7dd1\u306e\u6955\u5186\u3067\u793a\u3059\u3088\u3046\u306aA\u3042\u308b\u3044\u306fB\u30a2\u30c0\u30d7\u30bf\u30fc\u306e\u7247\u65b9\u3057\u304b\u6301\u305f\u306a\u3044\u7121\u52b9\u306a\u30e9\u30a4\u30d6\u30e9\u30ea\u306e\u751f\u6210\u3092\u6700\u5c0f\u9650\u306b\u6291\u3048\u307e\u3059\u3002\u7121\u52b9\u306a\u30e9\u30a4\u30d6\u30e9\u30ea\u3068\u6709\u52b9\u306a\u30e9\u30a4\u30d6\u30e9\u30ea\u304c\u6df7\u5728\u3059\u308b\u4e2d\u3067\u3001\u5206\u5149\u5149\u5ea6\u8a08\u306a\u3069\u3067\u30e9\u30a4\u30d6\u30e9\u30ea\u6fc3\u5ea6\u306e\u5b9a\u91cf\u3092\u884c\u3046\u3053\u3068\u306f\u4e0d\u5341\u5206\u3068\u3044\u3048\u307e\u3059<\/strong>\u3002
\n\"digitalpcr4-fig2\"<\/a>
\n\u30c7\u30b8\u30bf\u30ebPCR\u306f\u3001\u7279\u306bPCR\u30b9\u30c6\u30c3\u30d7\u3092\u542b\u307e\u306a\u3044\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb(Non-Standard Library)\u3067\u4f5c\u88fd\u3057\u305f\u30e9\u30a4\u30d6\u30e9\u30ea\u306e\u5b9a\u91cf\u306b\u7279\u306b\u6709\u52b9\u3067\u3059<\/strong>\u3002Non-Standard Library\u3067\u4f5c\u88fd\u3059\u308b\u3068\u3001\u7dd1\u306e\u6955\u5186\u3067\u793a\u3055\u308c\u3066\u3044\u308b\u3088\u3046\u306a\u3001\u7121\u52b9\u306a\u30e9\u30a4\u30d6\u30e9\u30ea\u306e\u5272\u5408\u304c\u3088\u308a\u9ad8\u304f\u306a\u308a\u307e\u3059\u3002<\/p>\n

Non-Standard Library\u306e\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\u3067\u4f5c\u88fd\u3055\u308c\u305f\u30e9\u30a4\u30d6\u30e9\u30ea\u306b\u304a\u3044\u3066\u3001\u5897\u5e45\u30b9\u30c6\u30c3\u30d7\u306f\u3001\u6b63\u3057\u304f\u4f5c\u88fd\u3055\u308c\u305f\u30e9\u30a4\u30d6\u30e9\u30ea\u306e\u6fc3\u7e2e\u306b\u6709\u7528\u3067\u3059\u3002\u73fe\u5728\u3001NGS\u30e9\u30a4\u30d6\u30e9\u30ea\u306e\u5b9a\u91cf\u306b\u4f7f\u7528\u3055\u308c\u3066\u3044\u308b\u65b9\u6cd5\u3068\u3057\u3066\u306f\u3001\u5206\u5149\u5149\u5ea6\u6cd5\u3084qPCR\u6cd5\u306a\u3069\u304c\u3042\u308a\u307e\u3059<\/strong>\u3002<\/p>\n

\u5206\u5149\u5149\u5ea6\u6cd5\u306f\u3001\u9577\u3044\u9593DNA\u3084RNA\u306e\u5b9a\u91cf\u306b\u4f7f\u7528\u3055\u308c\u3066\u304d\u307e\u3057\u305f\u3002\u3057\u304b\u3057\u3001\u7d2b\u5916\u7dda\u5438\u5149\u5ea6\u6cd5\u306b\u3088\u308b\u6e2c\u5b9a\u306f\u7279\u7570\u7684\u3067\u306f\u306a\u304f\u3001DNA\u3001RNA\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u9593\u306e\u533a\u5225\u304c\u3067\u304d\u307e\u305b\u3093\u3002\u307e\u305f\u6e2c\u5b9a\u5024\u306f\u3001\u904a\u96e2\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\u3001\u5869\u3001\u30a8\u30bf\u30ce\u30fc\u30eb\u3084\u30d5\u30a7\u30ce\u30fc\u30eb\u306a\u3069\u306e\u6709\u6a5f\u7269\u3084\u5869\u57fa\u7d44\u6210\u306e\u3070\u3089\u3064\u304d\u306a\u3069\u306b\u5f71\u97ff\u3092\u53d7\u3051\u3001\u5b9f\u969b\u306e\u6fc3\u5ea6\u3088\u308a\u9ad8\u3044\u6fc3\u5ea6\u304c\u793a\u3055\u308c\u308b\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u3002\u5206\u5149\u5149\u5ea6\u8a08\u306e\u611f\u5ea6\u306f\u3001\u4f4e\u6fc3\u5ea6\u306eDNA\u3084RNA\u5b9a\u91cf\u306b\u304a\u3044\u3066\u591a\u304f\u306e\u5834\u5408\u4e0d\u5341\u5206\u3067\u3059<\/strong>\u3002<\/p>\n

\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR \u306f\u3001NGS\u306e\u30e9\u30a4\u30d6\u30e9\u30ea\u5b9a\u91cf\u3092\u884c\u3046\u6709\u7528\u306a\u65b9\u6cd5\u3067\u3059\u304c\u3001\u30ea\u30d5\u30a1\u30ec\u30f3\u30b9\u30b5\u30f3\u30d7\u30eb\u3084\u6a19\u6e96\u66f2\u7dda\u306e\u4f5c\u6210\u304c\u5fc5\u8981\u3067\u3059<\/strong>\u3002\u30b9\u30eb\u30fc\u30d7\u30c3\u30c8\u304c\u6c42\u3081\u3089\u308c\u308b\u7814\u7a76\u5ba4\u3067\u3001\u7570\u306a\u308b\u7a2e\u985e\u306e\u30e9\u30a4\u30d6\u30e9\u30ea\u3092\u4e26\u884c\u3057\u3066\u30b7\u30fc\u30b1\u30f3\u30b9\u3092\u884c\u3046\u5834\u5408\u3001\u8907\u6570\u306e\u6a19\u6e96\u66f2\u7dda\u3092\u4f5c\u6210\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u304c\u3001\u4f5c\u696d\u304c\u7169\u96d1\u306b\u306a\u308a\u3001\u30b3\u30b9\u30c8\u3084\u6240\u8981\u6642\u9593\u304c\u5897\u3048\u3001\u30a8\u30e9\u30fc\u306e\u53ef\u80fd\u6027\u3092\u9ad8\u3081\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n

1\u3064\u306e\u5927\u304d\u306a\u6f5c\u5728\u7684\u30a8\u30e9\u30fc\u3068\u3057\u3066\u306f\u3001PCR\u30d0\u30a4\u30a2\u30b9<\/a>\u304c\u3042\u308a\u307e\u3059<\/strong>\u3002\u3053\u308c\u306f\u3001\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u306e\u5897\u5e45\u52b9\u7387\u306e\u5dee\u3084PCR\u306e\u5f8c\u534a\u306b\u751f\u3058\u308b\u5897\u5e45\u7523\u7269\u306b\u3088\u308b\u963b\u5bb3\u3067\u3059\u3002\u591a\u304f\u306e\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\u3067\u306f\u3001PCR\u30d0\u30a4\u30a2\u30b9\u3092\u907f\u3051\u308b\u305f\u3081\u306b\u5897\u5e45\u3092\u884c\u308f\u306a\u3044\u50be\u5411\u306b\u3042\u308a\u3001\u3053\u308c\u304c\u6b63\u3057\u304f\u4f5c\u88fd\u3055\u308c\u308b\u30e9\u30a4\u30d6\u30e9\u30ea\u306e\u5272\u5408\u306e\u4f4e\u4e0b\u306b\u3064\u306a\u304c\u3063\u3066\u3044\u307e\u3059\u3002<\/p>\n

\u7279\u306b\u3001\u5206\u5149\u5149\u5ea6\u8a08\u306f\u3001\u6b63\u3057\u304f\u4f5c\u88fd\u3055\u308c\u305f\u30e9\u30a4\u30d6\u30e9\u30ea\u3068\u6b63\u3057\u304f\u4f5c\u88fd\u3055\u308c\u306a\u304b\u3063\u305f\u30e9\u30a4\u30d6\u30e9\u30ea\u306e\u4e21\u65b9\u3092\u691c\u51fa\u3059\u308b\u305f\u3081\u3001\u30e9\u30a4\u30d6\u30e9\u30ea\u6fc3\u5ea6\u304c\u904e\u5927\u8a55\u4fa1\u3055\u308c\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n

Applied biosystems\u2122 QuantStudio\u2122 3D \u30c7\u30b8\u30bf\u30ebPCR\u30b7\u30b9\u30c6\u30e0<\/a>\u3092\u3001Ion Torrent\u2122\u3001\u3042\u308b\u3044\u306fIllumina\u00ae \u30e9\u30a4\u30d6\u30e9\u30ea\u5b9a\u91cf\u7528\u306eTaqMan\u2122 \u30a2\u30c3\u30bb\u30a4\u3068\u3068\u3082\u306b\u3054\u5229\u7528\u3044\u3044\u305f\u3060\u304f\u3068\u3001\u6b63\u3057\u304f\u4f5c\u88fd\u3055\u308c\u305f\u30e9\u30a4\u30d6\u30e9\u30ea\u306e\u307f\u3092\u691c\u51fa\u3067\u304d\u308b\u306e\u3067\u3001\u6fc3\u5ea6\u306e\u904e\u5927\u8a55\u4fa1\u306e\u554f\u984c\u3092\u8efd\u6e1b\u3057\u3001\u7cbe\u5bc6\u306a\u30e9\u30a4\u30d6\u30e9\u30ea\u5b9a\u91cf\u304c\u53ef\u80fd\u3067\u3059<\/strong>\u3002\u6b63\u78ba\u30fb\u7cbe\u5bc6\u306aNGS\u30e9\u30a4\u30d6\u30e9\u30ea\u306e\u5b9a\u91cf\u306f\u3001\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\u30c7\u30fc\u30bf\u306e\u53ce\u7387\u3092\u6700\u5927\u5316\u3059\u308b\u305f\u3081\u306b\u3082\u3001Ion Torrent\u2122\u3084\u4ed6\u306eNGS\u30ef\u30fc\u30af\u30d5\u30ed\u30fc<\/a>\u306b\u304a\u3044\u3066\u3082\u91cd\u8981\u306a\u30b9\u30c6\u30c3\u30d7\u3067\u3059\u3002<\/p>\n

QuantStudio\u2122 3D\u3092\u7528\u3044\u305f Ion Torrent\u2122 NGS\u30e9\u30a4\u30d6\u30e9\u30ea\u306e\u7cbe\u5bc6\u306a\u5b9a\u91cf<\/a><\/h2>\n

Ion Torrent \u30d7\u30e9\u30c3\u30c8\u30d5\u30a9\u30fc\u30e0\u306a\u3069\u3092\u7528\u3044\u305fNGS\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u306e\u4e2d\u3067\u3001\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u8abf\u88fd\u30b9\u30c6\u30c3\u30d7\u306f\u3001\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\u306e\u53ce\u7387\u3092\u6700\u9069\u5316\u3059\u308b\u305f\u3081\u306b\u91cd\u8981\u3067\u3059<\/strong>\u3002\u3053\u306e\u30b9\u30c6\u30c3\u30d7\u306f\u30e9\u30a4\u30d6\u30e9\u30ea\u306e\u30a4\u30f3\u30d7\u30c3\u30c8\u91cf\u306b\u5927\u304d\u304f\u5de6\u53f3\u3055\u308c\u307e\u3059\u3002\u30e9\u30a4\u30d6\u30e9\u30ea\u6fc3\u5ea6\u304c\u9ad8\u3059\u304e\u3066\u3082\u4f4e\u3059\u304e\u3066\u3082\u3001\u30c8\u30fc\u30bf\u30eb\u306e\u30ea\u30fc\u30c9\u6570\u304a\u3088\u3073\u30b9\u30eb\u30fc\u30d7\u30c3\u30c8\u306b\u30de\u30a4\u30ca\u30b9\u306e\u5f71\u97ff\u3092\u53ca\u307c\u3057\u307e\u3059\u3002<\/p>\n

\"digitalpcr4-fig3\"<\/a>

\u56f33 Ion OneTouch\u2122 2 \u30b7\u30b9\u30c6\u30e0\u3067\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u3092\u8abf\u6574\u3059\u308b\u524d\u306b\u3001QuantStudio 3D\u3067NGS\u30e9\u30a4\u30d6\u30e9\u30ea\u3092\u6b63\u78ba\u306b\u5b9a\u91cf\u3059\u308bNGS\u30ef\u30fc\u30af\u30d5\u30ed\u30fc<\/p><\/div>\n

QuantStudio 3D \u30c7\u30b8\u30bf\u30ebPCR\u30b7\u30b9\u30c6\u30e0\u3067\u306f\u3001PCR\u53cd\u5fdc\u6db2(\u5e0c\u91c8\u3057\u305f\u30b5\u30f3\u30d7\u30eb+ QuantStudio 3D dPCR Master Mix + TaqMan\u30a2\u30c3\u30bb\u30a4<\/a>)\u3092\u300120,000\u500b\u306e\u30a6\u30a7\u30eb\u3092\u6301\u3064\u30c1\u30c3\u30d7\u306b\u5206\u914d\u3057\u5bc6\u9589\u3057\u307e\u3059\u3002\u30bf\u30fc\u30b2\u30c3\u30c8\u5206\u5b50\u306e1\u30b3\u30d4\u30fc\u304c1\u30a6\u30a7\u30eb\u306b\u542b\u3080\u304b\u542b\u307e\u306a\u3044\u3088\u3046\u306b\u3001\u30b5\u30f3\u30d7\u30eb\u3092\u6700\u521d\u306b\u5e0c\u91c8\u3057\u3001\u30c7\u30e5\u30a2\u30eb\u30d5\u30e9\u30c3\u30c8\u30d6\u30ed\u30c3\u30af\u306e\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u2015\u3067\u5897\u5e45\u3092\u884c\u3044\u307e\u3059\u3002\u30bf\u30fc\u30b2\u30c3\u30c8\u5206\u5b50\u304c\u542b\u3080\u30a6\u30a7\u30eb\u306f\u30dd\u30b8\u30c6\u30a3\u30d6\u53cd\u5fdc\u3001\u542b\u307e\u306a\u3044\u30a6\u30a7\u30eb\u306f\u30cd\u30ac\u30c6\u30a3\u30d6\u53cd\u5fdc\u3068\u306a\u308a\u3001\u30cd\u30ac\u30c6\u30a3\u30d6\u53cd\u5fdc\u306e\u30a6\u30a7\u30eb\u306e\u5272\u5408\u3092\u7528\u3044\u3066\u30b5\u30f3\u30d7\u30eb\u4e2d\u306e\u30bf\u30fc\u30b2\u30c3\u30c8\u5206\u5b50\u3092\u5b9a\u91cf\u3057\u307e\u3059\u3002<\/p>\n

\"digitalpcr4-fig4\"<\/a>

\u56f34 QuantStudio 3D\u3068Ion OneTouch 2\u30c7\u30fc\u30bf\u306e\u76f8\u95a2\u95a2\u4fc2\u3002\u3053\u306eQC\u30c7\u30fc\u30bf\u306f\u3001\u6fc3\u7e2e\u524d\u306b4\u7a2e\u985e\u306e Ion Torrent \u30e9\u30a4\u30d6\u30e9\u30ea\u306b\u3064\u3044\u3066\u30d5\u30ed\u30fc\u30b5\u30a4\u30c8\u30e1\u30c8\u30ea\u30fc\u3092\u7528\u3044\u3066\u751f\u6210\u3002<\/p><\/div>\n

\u7d76\u5bfe\u5b9a\u91cf<\/strong>: \u6700\u7d42\u7684\u306a\u30e9\u30a4\u30d6\u30e9\u30ea\u6fc3\u5ea6\u3092\u6c42\u3081\u308b\u524d\u306b\u3001\u30e9\u30a4\u30d6\u30e9\u30ea\u30b5\u30f3\u30d7\u30eb\u306e\u5e0c\u91c8\u7387\u3068\u53cd\u5fdc\u6db2\u306e\u8abf\u5408\u6642(\u30c1\u30c3\u30d7\u4e0a\u3067\u306e)\u306e\u5e0c\u91c8\u7387\u306b\u57fa\u3065\u3044\u3066\u30c7\u30fc\u30bf\u3092\u8abf\u6574\u3057\u306a\u3051\u308c\u3070\u306a\u308a\u307e\u305b\u3093\u3002 \u3053\u306e\u30d7\u30ed\u30bb\u30b9\u306f\u3001QuantStudio\u2122 3D AnalysisSuite\u2122 Cloud \u30bd\u30d5\u30c8\u30a6\u30a7\u30a2<\/a>\u3092\u3054\u5229\u7528\u3044\u3044\u305f\u3060\u3051\u307e\u3059\u3002<\/p>\n

\u76f8\u95a2: Ion OneTouch 2 \u30b7\u30b9\u30c6\u30e0\u3067\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u30d3\u30fc\u30ba\u3092\u8abf\u88fd\u3059\u308b\u305f\u3081\u306b\u306f\u3001\u6fc3\u5ea6\u8a08\u7b97\u5024 (nM) \u306b\u57fa\u3065\u3044\u3066\u30e9\u30a4\u30d6\u30e9\u30ea\u3092\u5e0c\u91c8\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u30a8\u30f3\u30ea\u30c3\u30c1\u30e1\u30f3\u30c8\u3092\u884c\u3046\u524d\u306b\u3001Qubit\u00ae 3.0 Fluorometer<\/a>\u3092\u4f7f\u7528\u3057\u3066\u3001\u30b5\u30f3\u30d7\u30eb\u4e2d\u3067\u751f\u6210\u3055\u308c\u305f\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u30d3\u30fc\u30ba\u306e\u54c1\u8cea\u3092\u8a55\u4fa1\u3059\u308b\u305f\u3081\u306b\u54c1\u8cea\u7ba1\u7406\u306e\u30b9\u30c6\u30c3\u30d7\u304c\u5fc5\u8981\u3067\u3059\u3002\u30d3\u30fc\u30ba\u306e10\uff5e30%\u304c\u5897\u5e45\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u3068\u7d50\u5408\u3059\u308b\u3088\u3046\u306b\u30e9\u30a4\u30d6\u30e9\u30ea\u3092\u5e0c\u91c8\u3057\u305f\u3068\u304d\u306b\u3001\u6700\u9069\u306a\u30e9\u30a4\u30d6\u30e9\u30ea\u5b9a\u91cf\u304c\u3067\u304d\u307e\u3059\u3002\u3053\u306e\u63a8\u5968\u7bc4\u56f2\u5185\u306b\u5165\u308b\u30b5\u30f3\u30d7\u30eb\u304b\u3089\u306f\u3001\u304a\u304a\u3080\u306d\u8cea\u306e\u9ad8\u3044\u30c7\u30fc\u30bf\u304c\u5f97\u3089\u308c\u307e\u3059\u3002<\/p>\n

\u307e\u3068\u3081<\/h2>\n

\u6700\u9069\u306aNGS\u30c7\u30fc\u30bf\u53ce\u7387\u3092\u5f97\u308b\u306b\u306f\u3001\u8cea\u306e\u9ad8\u3044\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u8abf\u88fd\u304c\u5fc5\u8981\u3067\u3059\u3002\u30c7\u30b8\u30bf\u30ebPCR\u3067\u30e9\u30a4\u30d6\u30e9\u30ea\u5b9a\u91cf\u3092\u884c\u3046\u3053\u3068\u3067\u3001\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\u306b\u304b\u304b\u308b\u5168\u4f53\u7684\u306a\u30b3\u30b9\u30c8\u306e\u524a\u6e1b\u306b\u3064\u306a\u304c\u308a\u307e\u3059<\/strong>\u3002\u305d\u308c\u306f\u3001\u30c0\u30a6\u30f3\u30b9\u30c8\u30ea\u30fc\u30e0\u3067\u306e\u8ffd\u52a0\u4f5c\u696d\u3084\u30b9\u30c6\u30c3\u30d7\u306e\u7e70\u308a\u8fd4\u3057\u3092\u56de\u907f\u3057\u3001\u30b7\u30fc\u30b1\u30f3\u30b9\u306e\u54c1\u8cea\u3092\u78ba\u4fdd\u3067\u304d\u308b\u304b\u3089\u3067\u3059\u3002QuantStudio 3D\u30c7\u30b8\u30bf\u30ebPCR\u30b7\u30b9\u30c6\u30e0\u3068\u30e9\u30a4\u30d6\u30e9\u30ea\u306b\u7279\u7570\u7684\u306aTaqMan\u30a2\u30c3\u30bb\u30a4\u306f\u3001\u30b5\u30f3\u30d7\u30eb\u306e\u53d6\u6271\u3044\u306b\u304b\u304b\u308b\u624b\u9593\u3092\u6700\u5c0f\u9650\u306b\u6291\u3048\u3001\u6a19\u6e96\u66f2\u7dda\u3084\u30ea\u30d5\u30a1\u30ec\u30f3\u30b9\u30b5\u30f3\u30d7\u30eb\u3092\u4f7f\u7528\u3059\u308b\u3053\u3068\u306a\u304f\u3001\u9ad8\u7cbe\u5ea6\u306e\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u8abf\u88fd\u3092\u5b9f\u73fe\u3057\u305f\u3001\u304a\u624b\u9803\u3067\u4f7f\u3044\u6613\u3044\u30c7\u30b8\u30bf\u30ebPCR\u6cd5\u3092\u63d0\u4f9b\u3057\u307e\u3059\u3002<\/strong><\/p>\n

\u3010\u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3011\u30c7\u30b8\u30bf\u30ebPCR\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u30ac\u30a4\u30c9<\/h3>\n

\u30c7\u30b8\u30bf\u30ebPCR\u306b\u3088\u308b\u5b9a\u91cf\u304c\u6709\u7528\u306a\u8907\u6570\u306e\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306b\u3064\u3044\u3066\u7d39\u4ecb\u3057\u3066\u3044\u307e\u3059\u3002\u3053\u308c\u304b\u3089\u30c7\u30b8\u30bf\u30ebPCR\u5b9f\u9a13\u3092\u8003\u3048\u3066\u3044\u308b\u65b9\u306b\u6700\u9069\u3067\u3059\u3002<\/p>\n

\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u306f\u3053\u3061\u3089<\/a>
\n <\/p>\n

\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u7528\u306b\u306f\u4f7f\u7528\u3044\u305f\u3060\u3051\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

\u30c7\u30b8\u30bf\u30ebPCR (dPCR) 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