{"id":373,"date":"2016-04-21T00:00:00","date_gmt":"2016-04-20T15:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/digitalpcr5\/"},"modified":"2025-07-01T17:01:06","modified_gmt":"2025-07-01T08:01:06","slug":"digitalpcr5","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/digitalpcr5\/","title":{"rendered":"\u30ec\u30a2\u306a\u5909\u7570\u3092\u77ed\u6642\u9593\u3067\u691c\u51fa\uff01\u5909\u7570\u89e3\u6790\u306b\u304a\u3051\u308b\u30c7\u30b8\u30bf\u30ebPCR\u306e\u30e1\u30ea\u30c3\u30c8\u3068\u306f\uff1f"},"content":{"rendered":"<h2>\u30ec\u30a2\u306a\u5909\u7570\u306e\u691c\u51fa\u306f\u304c\u3093\u7814\u7a76\u306e\u5f37\u529b\u306a\u30c4\u30fc\u30eb<\/h2>\n<p><strong>\u304c\u3093\u306e\u7814\u7a76\u306b\u304a\u3044\u3066\u3001\u591a\u304f\u3092\u5360\u3081\u308b\u91ce\u751f\u578b\u7d30\u80de\u96c6\u56e3\u306e\u4e2d\u304b\u3089<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/pcr\/digital-pcr\/quantstudio-3d-applications\/sensitive-mutation-detection-taqman-liquid-biopsy-dpcr-assays.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_oc_s00_qpcr41_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">\u30ec\u30a2\u306a\u5909\u7570\u306e\u691c\u51fa<\/a>\u3092\u884c\u3046\u3053\u3068\u306f\u5927\u5909\u56f0\u96e3\u3067\u3059<\/strong>\u3002\u304c\u3093\u907a\u4f1d\u5b50\u3084\u304c\u3093\u6291\u5236\u907a\u4f1d\u5b50\u306e\u5909\u7570\u306e\u84c4\u7a4d\u306f\u3001\u816b\u760d\u5f62\u6210\u306e\u91cd\u8981\u306a\u8981\u56e0\u3067\u3042\u308a\u3001\u3054\u304f\u4e00\u90e8\u306e\u4f53\u7d30\u80de\u306b\u304a\u3051\u308b\u3053\u3046\u3057\u305f\u5909\u7570\u306e\u767a\u751f\u304c\u3001\u304c\u3093\u306e\u767a\u751f\u3084\u9032\u884c\u306e\u539f\u56e0\u3068\u306a\u308a\u5f97\u307e\u3059\u3002<\/p>\n<p><a href=\"http:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/sequencing\/sanger-sequencing.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_oc_s00_qpcr40_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">\u30ad\u30e3\u30d4\u30e9\u30ea\u96fb\u6c17\u6cf3\u52d5<\/a>\u3084<a href=\"http:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/pcr\/real-time-pcr.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_oc_s00_qpcr40_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR<\/a>\u3068\u3044\u3063\u305f\u4e00\u822c\u7684\u306aSNP\u30b8\u30a7\u30ce\u30bf\u30a4\u30d4\u30f3\u30b0\u306e\u6280\u8853\u306f\u300120%\u306e\u983b\u5ea6\u3092\u8d85\u3048\u308b (\u304a\u3088\u305d5\u3064\u306e\u7d30\u80de\u306b1\u3064\u306e\u5909\u7570) \u5909\u7570\u7d30\u80de\u306e\u691c\u51fa\u306b\u306f\u6709\u52b9\u3067\u3059\u3002\u3057\u304b\u3057\u3001<strong>\u901a\u5e38\u5909\u7570\u306f\u3054\u304f\u5c11\u6570\u306e\u7d30\u80de\u306b\u767a\u751f\u3059\u308b\u306e\u3067\u3001\u305d\u306e\u5909\u7570\u3092\u691c\u51fa\u3059\u308b\u305f\u3081\u306b\u306f\u3001\u9ad8\u3044\u30b7\u30b0\u30ca\u30eb\u30fb\u30ce\u30a4\u30ba\u6bd4\u3092\u6709\u3057\u3001\u507d\u967d\u6027\u7387\u306e\u4f4e\u3044\u30a2\u30c3\u30bb\u30a4\u304c\u5fc5\u8981\u3068\u306a\u308a\u307e\u3059<\/strong>\u3002\u3053\u308c\u3092\u5099\u3048\u3066\u3044\u308b\u306e\u304c\u30c7\u30b8\u30bf\u30ebPCR\u3067\u3059\u3002<strong>\u30b5\u30f3\u30d7\u30eb\u3092\u4f55\u5343\u3082\u306e\u500b\u5225\u306ePCR\u53cd\u5fdc\u306b\u5206\u5272\u3059\u308b\u3053\u3068\u3067\u3001\u53cd\u5fdc\u3054\u3068\u306e\u5206\u5b50\u306e\u7dcf\u6570\u306f\u5927\u5e45\u306b\u6e1b\u5c11\u3057\u3001\u5bfe\u8c61\u914d\u5217\u304c\u52b9\u679c\u7684\u306b\u6fc3\u7e2e\u3055\u308c\u3001\u91ce\u751f\u578b\u306e\u30d0\u30c3\u30af\u30b0\u30e9\u30f3\u30c9\u5206\u5b50\u304c\u5e0c\u91c8\u3055\u308c\u6392\u9664\u3055\u308c\u307e\u3059<\/strong>\u3002<\/p>\n<p>\u307e\u305f\u3001<strong>\u4e0d\u5747\u4e00\u306b\u6df7\u3056\u308a\u5408\u3063\u305f\u30b5\u30f3\u30d7\u30eb\u306e\u4e2d\u304b\u3089\u3001\u3054\u304f\u308f\u305a\u304b\u306a\u91cf\u306e\u6838\u9178\u306e\u691c\u51fa \uff08\u30a2\u30ec\u30eb\u306e\u691c\u51fa\u306a\u3069\uff09 \u306b\u3082\u3001\u30c7\u30b8\u30bf\u30ebPCR\u304c\u5f79\u7acb\u3061\u307e\u3059<\/strong>\u3002\u305f\u3068\u3048\u3070\u3001\u30c7\u30b8\u30bf\u30ebPCR\u3092\u7528\u3044\u3066\u3001\u816b\u760d\u30b5\u30f3\u30d7\u30eb\u306e\u308f\u305a\u304b\u306a\u7d30\u80de\u304c\u5909\u7570\u3092\u6709\u3057\u3066\u3044\u308b\u304b\u5426\u304b\u3092\u78ba\u8a8d\u3067\u304d\u307e\u3059\u3002<\/p>\n<p>TaqMan\u2122 \u30b1\u30df\u30b9\u30c8\u30ea\u3068<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/pcr\/digital-pcr.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_oc_s00_qpcr40_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">QuantStudio\u2122 3D \u30c7\u30b8\u30bf\u30ebPCR \u30b7\u30b9\u30c6\u30e0<\/a>\u3092\u7d44\u307f\u5408\u308f\u305b\u3066\u4f7f\u3046\u3053\u3068\u3067\u3001\u4f4e\u983b\u5ea6\u306e\u5909\u7570\u3092\u691c\u51fa\u3067\u304d\u308b\u3088\u3046\u306b\u306a\u308a\u307e\u3057\u305f\u3002<a href=\"http:\/\/www.thermofisher.com\/content\/dam\/LifeTech\/Documents\/XLS\/digital-pcr-rare-mutation-assay-id-list.xlsx?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_oc_s00_qpcr40_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">TaqMan\u00ae SNP Genotyping\u30a2\u30c3\u30bb\u30a4<\/a>\u3068\u3068\u3082\u306b\u3001<a href=\"https:\/\/www.thermofisher.com\/content\/dam\/LifeTech\/global\/life-sciences\/PCR\/qPCR\/pdfs\/quantstudio-3d-rare-mutation-analysis-solution.pdf?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_oc_s00_qpcr40_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">QuantStudio 3D rare mutation analysis solution<\/a>\u306f\u3001<strong>\u975e\u5e38\u306b\u983b\u5ea6\u306e\u4f4e\u3044\u5909\u7570\u306e\u691c\u51fa\u3068\u305d\u306e\u7d76\u5bfe\u5b9a\u91cf\u306b\u5fc5\u8981\u306a\u9ad8\u3044\u6027\u80fd\u3092\u5b9f\u73fe\u3057\u3066\u3044\u307e\u3059<\/strong>\u3002<\/p>\n<h2>\u30ec\u30a2\u306a\u5909\u7570\u3092\u77ed\u6642\u9593\u3067\u691c\u51fa\uff01 QuantStudio 3D \u30c7\u30b8\u30bf\u30ebPCR\u30b7\u30b9\u30c6\u30e0<\/h2>\n<p>QuantStudio 3D \u30c7\u30b8\u30bf\u30ebPCR\u30b7\u30b9\u30c6\u30e0\u306f\u3001<strong>\u304c\u3093\u7814\u7a76\u306a\u3069\u306e\u4f4e\u6fc3\u5ea6\u9818\u57df\u306e\u30b5\u30f3\u30d7\u30eb\u304b\u3089\u306e\u30ec\u30a2\u306a\u5909\u7570\u306e\u691c\u51fa\u3001\u5b9a\u91cf\u3092\u53ef\u80fd\u306b\u3057\u305f\u30b7\u30f3\u30d7\u30eb\u304b\u3064\u304a\u624b\u9803\u306a\u30c7\u30b8\u30bf\u30ebPCR\u30b7\u30b9\u30c6\u30e0<\/strong>\u3067\u3059\u3002QuantStudio 3D \u30c7\u30b8\u30bf\u30ebPCR\u30b7\u30b9\u30c6\u30e0\u306f\u3001\u9ad8\u5bc6\u5ea6\u306e\u30ca\u30ce\u6d41\u4f53\u5de5\u5b66\u306b\u57fa\u3065\u304f\u30c1\u30c3\u30d7\u30c6\u30af\u30ce\u30ed\u30b8\u30fc\u3092\u63a1\u7528\u3057\u3001\u5404\u3005\u72ec\u7acb\u3057\u305f20,000\u500b\u3082\u306e\u53cd\u5fdc\u30a6\u30a7\u30eb\u306b\u30b5\u30f3\u30d7\u30eb\u3092\u5206\u914d\u3057\u307e\u3059\u3002\u56fa\u4f53\u30b7\u30ea\u30b3\u30f3\u57fa\u677f\u306b\u523b\u307f\u8fbc\u307e\u308c\u305f\u5747\u4e00\u306a\u30b5\u30a4\u30ba\u306e\u5fae\u7d30\u30a6\u30a7\u30eb\u3092\u6709\u3059\u308b\u5c02\u7528\u30c1\u30c3\u30d7\u306b\u3088\u3063\u3066\u3001\u76f4\u63a5\u7684\u304b\u3064\u5b89\u5b9a\u3057\u305f\u30b5\u30f3\u30d7\u30eb\u5206\u914d\u304c\u53ef\u80fd\u3068\u306a\u308a\u30011\u56de\u306e\u30e9\u30f3\u3067\u6570\u5343\u3082\u306e\u30c7\u30fc\u30bf\u30dd\u30a4\u30f3\u30c8\u3092\u89e3\u6790\u3067\u304d\u307e\u3059\u3002\u9ad8\u5ea6\u306b\u5236\u5fa1\u3055\u308c\u305f\u30b5\u30f3\u30d7\u30eb\u306e\u30ed\u30fc\u30c7\u30a3\u30f3\u30b0\u306e\u30b7\u30b9\u30c6\u30e0\u3068\u5fae\u7d30\u30a6\u30a7\u30eb\u3092\u6301\u3064\u30c1\u30c3\u30d7\u306f\u5747\u4e00\u306a\u91cf\u3067\u53cd\u5fdc\u3092\u884c\u3044\u3001\u3088\u308a\u9ad8\u7cbe\u5ea6\u306a\u7d76\u5bfe\u5b9a\u91cf\u3092\u53ef\u80fd\u3068\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<h3>QuantStudio 3D \u30c7\u30b8\u30bf\u30ebPCR\u30b7\u30b9\u30c6\u30e0\u306eRare mutation analysis solution\u306e\u30e1\u30ea\u30c3\u30c8:<\/h3>\n<ul>\n<li>\u30c7\u30b8\u30bf\u30ebPCR\u306e\u6027\u80fd\u3092\u6700\u9069\u5316<\/li>\n<li>\u304c\u3093\u7814\u7a76\u3067\u6700\u3082\u3088\u304f\u5229\u7528\u3055\u308c\u308b\u4f53\u7d30\u80de\u5909\u7570\u3092\u30bf\u30fc\u30b2\u30c3\u30c8\u3068\u3057\u305f\u30c7\u30b8\u30bf\u30ebPCR\u3067\u30d0\u30ea\u30c7\u30fc\u30b7\u30e7\u30f3\u3092\u884c\u3063\u305fTaqMan SNP Genotyping\u30a2\u30c3\u30bb\u30a4\u3092\u63d0\u4f9b<\/li>\n<li>0.1%\u7a0b\u5ea6\u306e\u30ec\u30a2\u306a\u5909\u7570\u306e\u691c\u51fa\u30fb\u5b9a\u91cf\u3092\u5b9f\u73fe<\/li>\n<li>\u30b7\u30f3\u30b0\u30eb\u30c1\u30e5\u30fc\u30d6\u306e\u30d5\u30a9\u30fc\u30de\u30c3\u30c8\u306f\u91ce\u751f\u578b\u304a\u3088\u3073\u5909\u7570\u907a\u4f1d\u5b50\u306b\u5bfe\u5fdc<\/li>\n<li>\u9ad8\u5ea6\u306a\u30a2\u30eb\u30b4\u30ea\u30ba\u30e0\u306b\u3088\u308a\u3001\u30ec\u30a2\u306a\u5909\u7570\u306e\u5b9a\u91cf\u3092\u52b9\u7387\u5316<\/li>\n<\/ul>\n<h2>\u30d0\u30ea\u30c7\u30fc\u30b7\u30e7\u30f3\u6e08\u307f\u306eTaqMan SNP Genotyping\u30a2\u30c3\u30bb\u30a4\u3067\u3088\u308a\u9ad8\u3044\u7cbe\u5ea6\u3092\u5b9f\u73fe<\/h2>\n<p>TaqMan\u30a2\u30c3\u30bb\u30a4\u306e\u30b7\u30cb\u30a2\u30d7\u30ed\u30c0\u30af\u30c8\u30de\u30cd\u30b8\u30e3\u30fc Dr. Iain Russell \u304c\u30012014\u5e74\u30a2\u30e1\u30ea\u30ab\u4eba\u985e\u907a\u4f1d\u5b66\u4f1a (ASHG) \u5e74\u6b21\u7dcf\u4f1a\u3067\u306e\u30a4\u30f3\u30bf\u30d3\u30e5\u30fc\u3067\u3001<a href=\"https:\/\/www.youtube.com\/watch?v=1YwM2X_QmtY\" target=\"_blank\" rel=\"noopener noreferrer\">QuantStudio 3D \u30c7\u30b8\u30bf\u30ebPCR\u30b7\u30b9\u30c6\u30e0\u306b\u304a\u3051\u308b\u30ec\u30a2\u306a\u5909\u7570\u3092\u89e3\u6790\u3059\u308b\u30e1\u30ea\u30c3\u30c8\u306b\u3064\u3044\u3066\u8a9e\u308a\u307e\u3057\u305f<\/a>\u3002\u5177\u4f53\u7684\u306b\u306f\u3001QuantStudio 3D Rare mutation analysis solution\u306e\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u3068\u3001<strong>\u826f\u304f\u77e5\u3089\u308c\u3066\u3044\u308b\u4f53\u7d30\u80de\u5909\u7570 (EGFR\u3001BRAF\u3001KRAS\u3001PIK3CA\u3001JAK2) \u3092\u30bf\u30fc\u30b2\u30c3\u30c8\u3068\u3057\u305f\u3001\u30d0\u30ea\u30c7\u30fc\u30b7\u30e7\u30f3\u6e08\u307f\u306eTaqMan SNP Genotyping\u30a2\u30c3\u30bb\u30a4\u306b\u3064\u3044\u3066\u8a71\u3092\u3057\u307e\u3057\u305f\u3002\u3053\u308c\u3089\u306e\u30a2\u30c3\u30bb\u30a4\u306f\u3001QuantStudio 3D \u30c7\u30b8\u30bf\u30ebPCR\u30b7\u30b9\u30c6\u30e0\u3067\u30d0\u30ea\u30c7\u30fc\u30b7\u30e7\u30f3\u3055\u308c\u3066\u304a\u308a\u30010.1%\u307e\u3067\u306e\u30ec\u30a2\u306a\u5909\u7570\u3092\u5b9a\u91cf\u3067\u304d\u307e\u3059<\/strong>\u3002<\/p>\n<div id=\"attachment_1135\" style=\"width: 1005px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/digitalpcr5-fig1.jpg\" rel=\"attachment wp-att-1135\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-1135\" class=\"size-full wp-image-1135\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/digitalpcr5-fig1.jpg\" alt=\"digitalpcr5-fig1\" width=\"995\" height=\"512\" \/><\/a><p id=\"caption-attachment-1135\" class=\"wp-caption-text\">\u56f31 KRAS G12V\u5909\u7570\u3092\u30c7\u30b8\u30bf\u30ebPCR\u306e\u305f\u3081\u306b\u6700\u9069\u5316\u3055\u308c\u305fTaqMan\u00ae SNP Genotyping \u30a2\u30c3\u30bb\u30a4\u3092\u7528\u3044\u3001QuantStudio\u00ae 3D \u30c7\u30b8\u30bf\u30ebPCR\u30b7\u30b9\u30c6\u30e0\u3067\u691c\u51fa\u3057\u307e\u3057\u305f\u3002\u30b5\u30f3\u30d7\u30eb\u306f\u91ce\u751f\u578b\u306e\u30b2\u30ce\u30e0DNA\u306b\u308f\u305a\u304b\u306a\u91cf\u306e\u5909\u7570\u578b\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u6df7\u5408\u3057\u305f\u30b5\u30f3\u30d7\u30eb\u3067\u3059\u3002\u30bf\u30fc\u30b2\u30c3\u30c80.1%\u306f\u91ce\u751f\u578b\u30b2\u30ce\u30e0DNA\u306b0.1%\u306e\u5909\u7570\u304c\u542b\u307e\u308c\u308b\u3053\u3068\u3092\u610f\u5473\u3057\u307e\u3059\u3002\u5168\u3066\u306e\u30b5\u30f3\u30d7\u30eb\u306fQuantStudio\u00ae 3D \u30c7\u30b8\u30bf\u30ebPCR\u30b7\u30b9\u30c6\u30e0\u306eAnalysisSuite\u2122 Cloud Software\u306e\u300cRelative Quantification\u300d\u30e2\u30b8\u30e5\u30fc\u30eb\u3067\u89e3\u6790\u3092\u884c\u3044\u307e\u3057\u305f\u3002A) \u30bf\u30fc\u30b2\u30c3\u30c810%\u306e\u89e3\u6790\u7d50\u679c\u3002 \u691c\u51fa\u7d50\u679c\u306f10.384%\u3002\u4fe1\u983c\u533a\u9593\u306f8.235% &#8211; 13.076%\u3002 B) \u30bf\u30fc\u30b2\u30c3\u30c81%\u306e\u89e3\u6790\u7d50\u679c\u3002\u691c\u51fa\u7d50\u679c\u306f1.393%\u3002\u4fe1\u983c\u533a\u9593\u306f0.772% &#8211; 2.507%\u3002C) \u30bf\u30fc\u30b2\u30c3\u30c80.1%\u306e\u89e3\u6790\u7d50\u679c\u3002 \u691c\u51fa\u7d50\u679c\u306f0.142%\u3002\u4fe1\u983c\u533a\u9593\u306f0.064% &#8211; 0.310%\u3002D) \u91ce\u751f\u578b\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u30b5\u30f3\u30d7\u30eb\u306e\u89e3\u6790\u7d50\u679c\u3002<\/p><\/div>\n<p>\u30d0\u30ea\u30c7\u30fc\u30b7\u30e7\u30f3\u6e08\u307f\u306eSNP\u30b8\u30a7\u30ce\u30bf\u30a4\u30d4\u30f3\u30b0\u30a2\u30c3\u30bb\u30a4\u306f\u3001\u305d\u306e\u767a\u751f\u306e\u983b\u5ea6\u3084<a href=\"http:\/\/www.thermofisher.com\/order\/catalog\/product\/4475346?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_oc_s00_qpcr40_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Ion AmpliSeq\u2122 Cancer Hotspot Panel<\/a>\u306b\u8f09\u3063\u3066\u3044\u308b\u30bf\u30fc\u30b2\u30c3\u30c8\u3092\u3082\u3068\u306b\u9078\u629e\u3055\u308c\u307e\u3057\u305f\u3002\u30a2\u30c3\u30bb\u30a4\u306f\u30b7\u30f3\u30b0\u30eb\u30c1\u30e5\u30fc\u30d6\u3067\u3001\u91ce\u751f\u578b\u304a\u3088\u3073\u5909\u7570\u30a2\u30ec\u30eb\u306e\u4e21\u65b9\u306b\u5bfe\u5fdc\u3059\u308b\u30d7\u30e9\u30a4\u30de\u30fc\u3068\u30d7\u30ed\u30fc\u30d6\u304c\u542b\u307e\u308c\u3001<a href=\"http:\/\/tools.thermofisher.com\/content\/sfs\/brochures\/rare-mutation-analysis-using-qs-3d-digital-pcr-sys.pdf?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_oc_s00_qpcr40_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">12\u304a\u3088\u3073450\u53cd\u5fdc\u30b5\u30a4\u30ba<\/a>\u3067\u63d0\u4f9b\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<h2>\u81ea\u4fe1\u3092\u3082\u3063\u3066\u7d76\u5bfe\u5b9a\u91cf\u3092\u884c\u3046\u305f\u3081\u306b<\/h2>\n<p><a href=\"http:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/pcr\/digital-pcr\/quantstudio-3d-digital-pcr-system\/quantstudio-3d-software.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_oc_s00_qpcr40_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">QuantStudio\u2122 3D AnalysisSuite\u2122 Cloud \u30bd\u30d5\u30c8\u30a6\u30a7\u30a2<\/a>\u306b\u3088\u3063\u3066\u3001QuantStudio 3D \u30c7\u30b8\u30bf\u30eb PCR \u30b7\u30b9\u30c6\u30e0\u3067\u53ce\u96c6\u3057\u305f\u30c7\u30fc\u30bf\u304b\u3089\u3001\u30b5\u30f3\u30d7\u30eb\u306b\u304a\u3051\u308b\u5909\u7570\u306e\u5272\u5408\u3092\u5b9a\u91cf\u3067\u304d\u307e\u3059\u3002<\/p>\n<p>\u30ec\u30a2\u306a\u907a\u4f1d\u5b50\u3092\u691c\u51fa\u3059\u308b\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306b\u304a\u3044\u3066\u3001AnalysisSuite\u2122 \u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u306f\u3001\u500b\u3005\u306ePCR\u53cd\u5fdc\u306e\u7d50\u679c\u30924\u3064\u306e\u8c61\u9650\u306b\u5206\u985e\u3057\u307e\u3059\u3002\u5de6\u4e0a\u306e\u8c61\u9650\uff08\u9752\u8272\u306e\u70b9\uff09\u306f\u307b\u307c\u4f8b\u5916\u306a\u304f\u30a2\u30ec\u30ebA\u3001\u53f3\u4e0b\uff08\u8d64\u8272\u306e\u70b9\uff09\u306f\u307b\u307c\u4f8b\u5916\u306a\u304f\u30a2\u30ec\u30ebB\u3067\u3059\u3002\u7dd1\u306e\u70b9\u306f\u3001\u30a2\u30ec\u30ebA\u3068B\u4e21\u65b9\u3092\u542b\u3080\u30a6\u30a7\u30eb\u3092\u793a\u3057\u3001\u9ec4\u8272\u306e\u70b9\u306f\u5897\u5e45\u304c\u306a\u304b\u3063\u305f\u30a6\u30a7\u30eb\u3092\u793a\u3057\u307e\u3059\u3002<\/p>\n<div id=\"attachment_1136\" style=\"width: 518px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/digitalpcr5-fig2.jpg\" rel=\"attachment wp-att-1136\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-1136\" class=\"size-full wp-image-1136\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/digitalpcr5-fig2.jpg\" alt=\"digitalpcr5-fig2\" width=\"508\" height=\"351\" \/><\/a><p id=\"caption-attachment-1136\" class=\"wp-caption-text\">* \u3053\u308c\u3089\u306e%\u306f\u3001AnalysisSuite\u2122 \u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u304b\u3089\u306e\u30c7\u30fc\u30bf\u3092\u4f7f\u7528\u3057Excel\u3067\u7b97\u51fa\u3002<\/p><\/div>\n<p>\u4e0a\u306e\u30c7\u30fc\u30bf\u306f\u3001\u5171\u540c\u7814\u7a76\u8005\u3067\u3042\u308bDr. Antonio Jimenez-Velasco (\u30b9\u30da\u30a4\u30f3\u3001\u30de\u30e9\u30ac\u3001\u30ab\u30eb\u30ed\u30b9\u30cf\u30e4\u75c5\u9662) \u304b\u3089\u3001\u9aa8\u9ac4\u79fb\u690d\u5f8c\u306e\u767d\u8840\u75c5\u60a3\u8005\u306e\u30b5\u30f3\u30d7\u30eb\u306e\u63d0\u4f9b\u3092\u53d7\u3051\u3001QuantStudio 3D \u30c7\u30b8\u30bf\u30eb PCR \u30b7\u30b9\u30c6\u30e0\u3067\u30ad\u30e1\u30ea\u30ba\u30e0\u306e\u30e2\u30cb\u30bf\u30ea\u30f3\u30b0\u3092\u884c\u3063\u305f\u4f8b\u3067\u3059\u3002\u533a\u5225\u304c\u5bb9\u6613\u306a\u30ec\u30b7\u30d4\u30a8\u30f3\u30c8\u7279\u7570\u7684\u306a\u907a\u4f1d\u5b50\u3092\u691c\u51fa\u3059\u308b\u30a2\u30c3\u30bb\u30a4\u3068\u5185\u90e8\u6a19\u6e96\u306e2\u7a2e\u985e\u306e\u30a2\u30c3\u30bb\u30a4\u3092\u7528\u3044\u3066\u5b9a\u91cf\u3092\u884c\u3044\u307e\u3057\u305f\u3002\u8840\u6db2\u30b5\u30f3\u30d7\u30eb\u306f\u3001\u30c9\u30ca\u30fc\u304a\u3088\u3073\u30ec\u30b7\u30d4\u30a8\u30f3\u30c8\u304b\u3089\u79fb\u690d\u524d\u3001\u304a\u3088\u3073\u79fb\u690d\u5f8c\u306b\u6570\u56de\u63a1\u53d6\u3055\u308c\u307e\u3057\u305f\u3002<\/p>\n<p>\u30b0\u30e9\u30d5\u306f\u3001\u30ec\u30b7\u30d4\u30a8\u30f3\u30c8\u306e\u30e2\u30cb\u30bf\u30ea\u30f3\u30b0\u7d50\u679c\u3092\u793a\u3057\u3066\u3044\u307e\u3059\u3002\u9aa8\u9ac4\u79fb\u690d\u524d (Pre-SCT)\u3001\u5faa\u74b0\u7d30\u80de\u306e\u907a\u4f1d\u5b50\u578b\u306f\u3001100%\u30db\u30b9\u30c8\u306e\u907a\u4f1d\u5b50\u578b\u3067\u3057\u305f\u3002\u30c9\u30ca\u30fc\u306f\u30ec\u30b7\u30d4\u30a8\u30f3\u30c8\u306e\u30a2\u30ec\u30eb\u3092\u7d040.1%\u6709\u3057\u3066\u3044\u307e\u3057\u305f\u300262\u65e5\u3001147\u65e5\u3001174\u65e5\u3068\u6642\u9593\u304c\u7d4c\u3064\u3068\u3001\u30ec\u30b7\u30d4\u30a8\u30f3\u30c8\u7531\u6765\u306e\u907a\u4f1d\u5b50\u578b\u306e\u5272\u5408\u304c\u4f4e\u304f\u306a\u3063\u3066\u3044\u308b\u306e\u304c\u308f\u304b\u308a\u307e\u3059\u3002\u3057\u304b\u3057\u3001192\u65e5\u5f8c\u304b\u3089\u306f\u4e0a\u6607\u3092\u59cb\u3081\u3001\u30ec\u30b7\u30d4\u30a8\u30f3\u30c8\u7531\u6765\u306e\u7d30\u80de\u304c\u9aa8\u9ac4\u3092\u518d\u69cb\u7bc9\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u308f\u304b\u308a\u307e\u3059\u3002 \u30a8\u30e9\u30fc\u30d0\u30fc\u306f\u3001\u7b97\u51fa\u3055\u308c\u305f95%\u4fe1\u983c\u533a\u9593\u3092\u8868\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>\u4e0a\u8ff0\u306e\u5171\u540c\u7814\u7a76\u8005\u306f\u3001<strong>\u5f93\u6765\u306ePCR\u6280\u8853\u3067\u306f192\u65e5\u3088\u308a\u524d\u306e\u6bb5\u968e\u3067\u3053\u306e\u30ad\u30e1\u30ea\u30ba\u30e0\u3092\u691c\u51fa\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u305b\u3093\u3067\u3057\u305f\u3002\u512a\u308c\u305f\u611f\u5ea6\u3092\u3082\u3064\u30c7\u30b8\u30bf\u30ebPCR\u306f\u3001\u5f93\u6765\u306ePCR\u6280\u8853\u3088\u308a\u65e9\u3044\u6bb5\u968e\u3067\u30ad\u30e1\u30ea\u30ba\u30e0 \uff08\u5143\u306e\u60a3\u8005\u7531\u6765\u306e\u907a\u4f1d\u5b50\u306e\u518d\u767a\uff09\u3092\u691c\u51fa\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3057\u305f<\/strong>\u3002<\/p>\n<p>Note\uff1a\u30ec\u30b7\u30d4\u30a8\u30f3\u30c8\u3068\u30c9\u30ca\u30fc\u4e21\u65b9\u306e\u7d30\u80de\u30b5\u30f3\u30d7\u30eb\u304c\u9aa8\u9ac4\u306b\u5b58\u5728\u3059\u308b\u3068\u304d\u3001\u767d\u8840\u75c5\u306e\u30b5\u30f3\u30d7\u30eb\u306b\u304a\u3044\u3066\u6df7\u5408\u30ad\u30e1\u30e9\u304c\u751f\u3058\u307e\u3059\u3002\u5e79\u7d30\u80de\u79fb\u690d\u5f8c\u306f\u3001\u30ad\u30e1\u30ea\u30ba\u30e0\u89e3\u6790\u304c\u5b9a\u671f\u7684\u306b\u884c\u308f\u308c\u307e\u3059\u3002<\/p>\n<h2>\u307e\u3068\u3081<\/h2>\n<p>\u30c7\u30b8\u30bf\u30ebPCR\u306b\u3088\u308b\u30ec\u30a2\u306a\u5909\u7570\u306e\u691c\u51fa\u30fb\u5b9a\u91cf\u304c\u7686\u69d8\u306e\u7814\u7a76\u306e\u304a\u5f79\u306b\u7acb\u3061\u3001\u4eca\u56de\u3054\u7d39\u4ecb\u3057\u305f\u30ea\u30f3\u30af\u304c\u30c7\u30b8\u30bf\u30eb PCR\u306e\u3055\u3089\u306a\u308b\u7406\u89e3\u306b\u3064\u306a\u304c\u308b\u3053\u3068\u3092\u9858\u3063\u3066\u3044\u307e\u3059\u3002\u307e\u3068\u3081\u306b\u306a\u308a\u307e\u3059\u304c\u3001QuantStudio 3D \u30c7\u30b8\u30bf\u30ebPCR\u30b7\u30b9\u30c6\u30e0\u306eRare mutation analysis solution\u306f\u3001\u4ee5\u4e0b\u306e\u7279\u9577\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<ul>\n<li>\u30c7\u30b8\u30bf\u30ebPCR\u306e\u6027\u80fd\u3092\u6700\u9069\u5316<\/li>\n<li>\u304c\u3093\u7814\u7a76\u3067\u6700\u3082\u3088\u304f\u5229\u7528\u3055\u308c\u308b\u4f53\u7d30\u80de\u5909\u7570\u3092\u30bf\u30fc\u30b2\u30c3\u30c8\u3068\u3057\u305f\u30c7\u30b8\u30bf\u30ebPCR\u3067\u30d0\u30ea\u30c7\u30fc\u30b7\u30e7\u30f3\u3092\u884c\u3063\u305fTaqMan SNP Genotyping\u30a2\u30c3\u30bb\u30a4\u3092\u63d0\u4f9b<\/li>\n<li>0.1%\u7a0b\u5ea6\u306e\u30ec\u30a2\u306a\u5909\u7570\u306e\u691c\u51fa\u30fb\u5b9a\u91cf\u3092\u5b9f\u73fe<\/li>\n<li>\u30b7\u30f3\u30b0\u30eb\u30c1\u30e5\u30fc\u30d6\u306e\u30d5\u30a9\u30fc\u30de\u30c3\u30c8\u306f\u91ce\u751f\u578b\u304a\u3088\u3073\u5909\u7570\u907a\u4f1d\u5b50\u306b\u5bfe\u5fdc<\/li>\n<li>\u9ad8\u5ea6\u306a\u30a2\u30eb\u30b4\u30ea\u30ba\u30e0\u306b\u3088\u308a\u3001\u30ec\u30a2\u306a\u5909\u7570\u306e\u5b9a\u91cf\u3092\u52b9\u7387\u5316<\/li>\n<\/ul>\n<h3>\u3010\u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3011\u30c7\u30b8\u30bf\u30ebPCR\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u30ac\u30a4\u30c9<\/h3>\n<p>\u30c7\u30b8\u30bf\u30ebPCR\u306b\u3088\u308b\u5b9a\u91cf\u304c\u6709\u7528\u306a\u8907\u6570\u306e\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306b\u3064\u3044\u3066\u7d39\u4ecb\u3057\u3066\u3044\u307e\u3059\u3002\u3053\u308c\u304b\u3089\u30c7\u30b8\u30bf\u30ebPCR\u5b9f\u9a13\u3092\u8003\u3048\u3066\u3044\u308b\u65b9\u306b\u6700\u9069\u3067\u3059\u3002<\/p>\n<p><a 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