{"id":381,"date":"2016-05-12T00:00:00","date_gmt":"2016-05-11T15:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/digitalpcr6\/"},"modified":"2025-07-01T17:08:10","modified_gmt":"2025-07-01T08:08:10","slug":"digitalpcr6","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/digitalpcr6\/","title":{"rendered":"\u7d76\u5bfe\u5b9a\u91cf\u3084\u30ec\u30a2\u306a\u914d\u5217\u306e\u691c\u51fa\u304c\u53ef\u80fd\u306a\u30c7\u30b8\u30bf\u30ebPCR\u306e\u5229\u70b9\u3092\u6d3b\u7528\u3057\u305f\u69d8\u3005\u306a\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306e\u4e8b\u4f8b\u307e\u3068\u3081"},"content":{"rendered":"

\u30c7\u30b8\u30bf\u30ebPCR\u306f\u9ad8\u611f\u5ea6\u30fb\u9ad8\u7cbe\u5ea6\u306a\u7d76\u5bfe\u5b9a\u91cf\u3092\u884c\u3046\u3053\u3068\u304c\u3067\u304d\u308b\u306e\u3067\u3001\u69d8\u3005\u306a\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306b\u304a\u3051\u308b\u30a2\u30c3\u30bb\u30a4\u306e\u30d1\u30d5\u30a9\u30fc\u30de\u30f3\u30b9\u306e\u6700\u9069\u5316\u3092\u6709\u52b9\u306b\u304a\u3053\u306a\u3046\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u305f\u3068\u3048\u3070\u3001\u5fae\u91cf\u306e\u75c5\u539f\u83cc\u306e\u691c\u51fa\u5b9a\u91cf<\/a>\u3067\u306f\u75c5\u539f\u83cc\u306e\u30b3\u30d4\u30fc\u6570\u3092\u76f4\u63a5\u5f97\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059<\/strong>\u3002\u907a\u4f1d\u5b50\u7d44\u307f\u63db\u3048\u4f5c\u7269(GMO)\u306e\u691c\u51fa<\/a>\u3067\u306f\u3001\u308f\u305a\u304b1\u500b\u306e\u7a2e\u306e\u6df7\u5165\u3082\u691c\u51fa\u53ef\u80fd\u306a\u611f\u5ea6\u304c\u5f97\u3089\u308c\u307e\u3059<\/strong>\u3002\u307e\u305f\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u5b9f\u9a13\u306b\u4f7f\u7528\u3059\u308b\u6a19\u6e96\u30b5\u30f3\u30d7\u30eb\u306e\u7d76\u5bfe\u5b9a\u91cf<\/a>\u3092\u884c\u3046\u3053\u3068\u3082\u3067\u304d\u307e\u3059<\/strong>\u3002<\/p>\n

QuantStudio 3D \u30c7\u30b8\u30bf\u30ebPCR\u30b7\u30b9\u30c6\u30e0<\/a>\u3068\u76f4\u611f\u7684\u306a\u30e6\u30fc\u30b6\u30fc\u30a4\u30f3\u30bf\u30fc\u30d5\u30a7\u30fc\u30b9\u3092\u5099\u3048\u305f\u30c7\u30fc\u30bf\u89e3\u6790\u30c4\u30fc\u30ebQuantStudio 3D AnalysisSuite Cloud \u30bd\u30d5\u30c8\u30a6\u30a7\u30a2<\/a>\u306f\u3001\u75c5\u539f\u4f53\u3084\u30a6\u30a3\u30eb\u30b9\u30b3\u30d4\u30fc\u6570\u306e\u7d76\u5bfe\u5b9a\u91cf\u306a\u3069\u3001\u3055\u307e\u3056\u307e\u306a\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u3092\u30b5\u30dd\u30fc\u30c8\u3057\u307e\u3059\u3002<\/p>\n

QuantStudio 3D \u30c7\u30b8\u30bf\u30ebPCR\u30b7\u30b9\u30c6\u30e0\u306f\u3001TaqMan \u304a\u3088\u3073 SYBR \u30b1\u30df\u30b9\u30c8\u30ea\u306e\u4e21\u65b9\u3092\u691c\u51fa\u3067\u304d\u307e\u3059\u3002TaqMan\u30b1\u30df\u30b9\u30c8\u30ea\u306f\u5b9a\u91cfPCR\u306b\u304a\u3051\u308b\u507d\u967d\u6027\u306e\u30ea\u30b9\u30af\u3092\u6700\u5c0f\u9650\u306b\u6291\u3048\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002QuantStudio 3D \u30c7\u30b8\u30bf\u30ebPCR\u3067\u306f\u3001800\u4e07\u7a2e\u985e\u3092\u8d85\u3048\u308b\u30d7\u30ec\u30c7\u30b6\u30a4\u30f3\u306eTaqMan\u30a2\u30c3\u30bb\u30a4\u304c\u3054\u5229\u7528\u306b\u306a\u308c\u307e\u3059\u3002<\/p>\n

\u308f\u305a\u304b\u306a\u6642\u9593\u3067\u7d76\u5bfe\u5b9a\u91cf\u3092\u53ef\u80fd\u306b\u3059\u308bQuantStudio 3D \u30c7\u30b8\u30bf\u30ebPCR\u30b7\u30b9\u30c6\u30e0<\/h2>\n

QuantStudio 3D \u30c7\u30b8\u30bf\u30ebPCR\u30b7\u30b9\u30c6\u30e0<\/a>\u306f\u3001\u30b7\u30f3\u30d7\u30eb\u304b\u3064\u304a\u624b\u9803\u306a\u30c7\u30b8\u30bf\u30ebPCR\u30d7\u30e9\u30c3\u30c8\u30d5\u30a9\u30fc\u30e0\u3067\u3059\u3002\u5358\u4e00\u5206\u5b50\u3092100\u4e07\u500d\u4ee5\u4e0a\u306b\u5897\u5e45\u3067\u304d\u308b\u306e\u3067\u3001\u9ad8\u3044\u7cbe\u5ea6\u3068\u611f\u5ea6\u3067\u6838\u9178\u306e\u691c\u51fa\u3084\u30ec\u30a2\u5909\u7570\u306e\u7d76\u5bfe\u5b9a\u91cf\u304c\u53ef\u80fd<\/strong>\u3067\u3059\u3002<\/p>\n

QuantStudio 3D \u30c7\u30b8\u30bf\u30ebPCR\u30b7\u30b9\u30c6\u30e0\u306f\u3001\u30ca\u30ce\u6d41\u4f53\u5de5\u5b66\u306b\u57fa\u3065\u304f\u9ad8\u5bc6\u5ea6\u306e\u30c1\u30c3\u30d7\u30c6\u30af\u30ce\u30ed\u30b8\u30fc\u3092\u63a1\u7528\u3057\u3001\u72ec\u7acb\u3057\u305f20,000\u500b\u306e\u53cd\u5fdc\u30a6\u30a7\u30eb\u306b\u30b5\u30f3\u30d7\u30eb\u3092\u5206\u914d\u3057\u307e\u3059\u3002QuantStudio 3D \u30c7\u30b8\u30bf\u30ebPCR \u30c1\u30c3\u30d7<\/a>\u4e0a\u306b\u306f\u56fa\u4f53\u30b7\u30ea\u30b3\u30f3\u57fa\u677f\u306b\u523b\u307f\u8fbc\u307e\u308c\u305f\u5747\u4e00\u30b5\u30a4\u30ba\u306e\u30a6\u30a7\u30eb\u304c\u3042\u308b\u306e\u3067\u3001\u76f4\u63a5\u7684\u304b\u3064\u5b89\u5b9a\u3057\u305f\u30b5\u30f3\u30d7\u30eb\u5206\u914d\u304c\u53ef\u80fd\u3067\u3059\u3002\u9ad8\u5ea6\u306b\u5236\u5fa1\u3055\u308c\u305f\u30b5\u30f3\u30d7\u30eb\u30ed\u30fc\u30c7\u30a3\u30f3\u30b0\u30b7\u30b9\u30c6\u30e0\u3068\u5fae\u7d30\u30a6\u30a7\u30eb\u306e\u30c1\u30c3\u30d7\u306e\u7d44\u307f\u5408\u308f\u305b\u306b\u3088\u308a\u3001\u9ad8\u7cbe\u5ea6\u306a\u7d76\u5bfe\u5b9a\u91cf\u3092\u5b9f\u73fe\u3057\u307e\u3059\u3002<\/p>\n

QuantStudio 3D \u30c7\u30b8\u30bf\u30ebPCR\u30b7\u30b9\u30c6\u30e0\u306f\u3001\u30b3\u30d4\u30fc\u6570\/\u03bcL\u306e\u30c7\u30fc\u30bf\u3092\u7b97\u51fa\u3059\u308b\u3068\u3068\u3082\u306b\u3001\u305d\u306e\u30c7\u30fc\u30bf\u306e\u54c1\u8cea\u3092\u8a55\u4fa1\u3057\u307e\u3059\u3002\u30c7\u30fc\u30bf\u54c1\u8cea\u304c\u60aa\u3044\u5834\u5408\u3084\u4e0d\u5408\u683c\u306e\u5834\u5408\u306f\u3001QuantStudio 3D AnalysisSuite Cloud \u30bd\u30d5\u30c8\u30a6\u30a7\u30a2<\/a>\u30672\u6b21\u89e3\u6790\u3092\u884c\u3046\u969b\u306b\u3001\u78ba\u8a8d\u3092\u4fc3\u3059\u30d5\u30e9\u30b0\u304c\u4ed8\u52a0\u3055\u308c\u307e\u3059\u3002<\/p>\n

\u5fae\u91cf\u306e\u75c5\u539f\u83cc\u306e\u691c\u51fa\u30fb\u5b9a\u91cf<\/h2>\n

\u6c5a\u67d3\u98df\u54c1\u3084\u6c5a\u67d3\u6c34\u306b\u542b\u307e\u308c\u308b\u7d30\u83cc\u3084\u30a6\u30a3\u30eb\u30b9\u6027\u75c5\u539f\u83cc\u306e\u30d2\u30c8\u3078\u306e\u5f71\u97ff\u3092\u6e1b\u3089\u3059\u305f\u3081\u306b\u306f\u3001\u5fae\u91cf\u306e\u75c5\u539f\u83cc\u306e\u691c\u51fa\u30fb\u5b9a\u91cf<\/a>\u3092\u3067\u304d\u308b\u3060\u3051\u65e9\u3044\u6bb5\u968e\u3067\u884c\u3046\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n

\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306f\u3001\u75c5\u539f\u83cc\u306e\u691c\u51fa\u306b\u304a\u3044\u3066\u6709\u7528\u306a\u30c4\u30fc\u30eb\u3067\u3059\u304c\u3001\u98df\u54c1\u3084\u6c34\u8cea\u691c\u67fb\u3067\u7528\u3044\u3089\u308c\u308b\u30b5\u30f3\u30d7\u30eb\u306b\u306fPCR\u963b\u5bb3\u7269\u8cea\u304c\u542b\u307e\u308c\u3066\u3044\u308b\u3053\u3068\u304c\u3042\u308a\u3001\u305d\u306e\u5f71\u97ff\u3092\u53d7\u3051\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059<\/strong>\u3002\u4e00\u65b9\u3001\u30c7\u30b8\u30bf\u30ebPCR\u306f\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3068\u6bd4\u8f03\u3057\u3066\u3001\u963b\u5bb3\u7269\u8cea\u306e\u5f71\u97ff\u3092\u53d7\u3051\u306b\u304f\u3044\u6280\u8853<\/strong>\u3067\u3059\u3002\u30c7\u30b8\u30bf\u30ebPCR\u306f\u3001\u5fae\u7d30\u306a\u30a6\u30a7\u30eb\u306b\u30b5\u30f3\u30d7\u30eb\u3092\u5206\u914d\u3059\u308b\u306e\u3068\u540c\u6642\u306b\u963b\u5bb3\u7269\u8cea\u3092\u5e0c\u91c8\u3057\u307e\u3059\u3002\u3055\u3089\u306b\u5897\u5e45\u91cf\u3067\u306f\u306a\u304f\u3001\u5206\u914d\u3055\u308c\u305f1\u30b3\u30d4\u30fc\u306e\u30b5\u30f3\u30d7\u30eb\u306ePCR\u5897\u5e45\u306e\u6709\u7121\u304b\u3089\u7d76\u5bfe\u5b9a\u91cf\u3092\u884c\u3044\u307e\u3059\u3002\u5f93\u3063\u3066\u3001PCR\u52b9\u7387\u304c\u640d\u306a\u308f\u308c\u308b\u3088\u3046\u306a\u5834\u5408\u306b\u304a\u3044\u3066\u3082\u3001\u305d\u306e\u5f71\u97ff\u3092\u53d7\u3051\u306b\u304f\u3044\u30c7\u30b8\u30bf\u30ebPCR\u306f\u9ad8\u7cbe\u5ea6\u306a\u5b9a\u91cf\u304c\u53ef\u80fd\u3067\u3059\u3002\u307e\u305f\u3001\u30c7\u30b8\u30bf\u30ebPCR\u306f\u30ea\u30d5\u30a1\u30ec\u30f3\u30b9\u30b5\u30f3\u30d7\u30eb\u3084\u6a19\u6e96\u66f2\u7dda\u304c\u4e0d\u8981\u306a\u306e\u3067\u3001\u30ea\u30d5\u30a1\u30ec\u30f3\u30b9\u30b5\u30f3\u30d7\u30eb\u3092\u5165\u624b\u3057\u306b\u304f\u3044\u75c5\u539f\u83cc\u306e\u7d76\u5bfe\u5b9a\u91cf\u306b\u304a\u3044\u3066\u3082\u6709\u7528<\/strong>\u3067\u3059\u3002<\/p>\n

\"digitalpcr6-fig1\"<\/a>

X\u8ef8\uff1a\u30d5\u30df\u30f3\u9178\u6fc3\u5ea6, Y\u8ef8\uff1a\u5b9a\u91cf\u3057\u305f\u6fc3\u5ea6<\/p><\/div>\n

\u74b0\u5883\u4e2d\u306b\u3088\u304f\u898b\u3089\u308c\u308bPCR\u963b\u5bb3\u7269\u8cea\u3067\u3042\u308b\u30d5\u30df\u30f3\u9178\u3092\u6dfb\u52a0\u3057\u3066\u3001\u3042\u308b\u75c5\u539f\u83cc\u306e\u6fc3\u5ea6\u6e2c\u5b9a\u3092\u884c\u3044\u307e\u3057\u305f\u3002\u30d5\u30df\u30f3\u9178\u306e\u6fc3\u5ea6\u304c\u9ad8\u304f\u306a\u308b\u3068\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\uff08\u30b0\u30ec\u30fc\u306e\u30e9\u30a4\u30f3\uff09\u306e\u6e2c\u5b9a\u5024\u306f\u3001\u5927\u304d\u304f\u4e0b\u304c\u308a\u307e\u3057\u305f\u3002\u307e\u305f\u3001\u30c7\u30b8\u30bf\u30ebPCR\u306e\u6e2c\u5b9a\u5024\uff08\u9752\u3044\u30e9\u30a4\u30f3\uff09\u306e\u30a8\u30e9\u30fc\u30d0\u30fc\u306f\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3068\u6bd4\u8f03\u3057\u3066\u975e\u5e38\u306b\u5c0f\u3055\u3044\u3053\u3068\u304c\u308f\u304b\u308a\u307e\u3059\u3002<\/p>\n

GMO\u3092\u9ad8\u611f\u5ea6\u306b\u691c\u51fa<\/h2>\n

\u690d\u7269\u306e\u8868\u73fe\u578b\u306e\u907a\u4f1d\u7684\u306a\u8907\u96d1\u3055\u3092\u7406\u89e3\u3059\u308b\u306e\u306f\u81f3\u96e3\u306e\u696d\u3067\u3059\u3002GMO\u4f5c\u7269\u306e\u4e16\u754c\u7684\u306a\u666e\u53ca\u3068\u3068\u3082\u306b\u3001\u98df\u54c1\u306e\u5b89\u5168\u6027\u306e\u305f\u3081\u306b\u6b63\u78ba\u306a\u8868\u793a\u7fa9\u52d9\u304c\u8ab2\u3055\u308c\u3001\u9ad8\u7cbe\u5ea6\u306aGMO\u691c\u51fa<\/a>\u306e\u5fc5\u8981\u6027\u304c\u9ad8\u307e\u3063\u3066\u3044\u307e\u3059\u3002<\/p>\n

\u5f93\u6765\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR \u306f\u690d\u7269\u306e\u907a\u4f1d\u5b50\u767a\u73fe\u5b9a\u91cf\u306b\u5e83\u304f\u4f7f\u7528\u3055\u308c\u3066\u304d\u307e\u3057\u305f\u3002\u3057\u304b\u3057\u3001\u963b\u5bb3\u7269\u8cea\u306e\u5f71\u97ff\u3092\u53d7\u3051\u308b\u3068\u30bf\u30fc\u30b2\u30c3\u30c8\u907a\u4f1d\u5b50\u306e\u6fc3\u5ea6\u304c\u4f4e\u304f\u306a\u308b\u3053\u3068\u304c\u4e88\u60f3\u3055\u308c\u307e\u3059<\/strong>\u3002\u305d\u306e\u305f\u3081\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306b\u3088\u308b\u690d\u7269\u306e\u5909\u7570\u306e\u5b9a\u91cf\u3084GMO\u306e\u691c\u51fa\u306f\u56f0\u96e3\u306a\u5834\u5408\u304c\u3042\u308a\u307e\u3057\u305f\u3002<\/p>\n

\u4e00\u65b9\u3001\u30c7\u30b8\u30bf\u30ebPCR\u306f\u3001GMO\u306e\u691c\u51fa\u3084\u690d\u7269\u306e\u5909\u7570\u907a\u4f1d\u5b50\u306e\u5b9a\u91cf\u306b\u7406\u60f3\u7684\u306a\u30b7\u30b9\u30c6\u30e0\u3067\u3059\u3002\u306a\u305c\u306a\u3089\u3001\u30c7\u30b8\u30bf\u30ebPCR\u306b\u3088\u308b\u30b5\u30f3\u30d7\u30eb\u4e2d\u306e\u7279\u5b9a\u306eSNPs\u3084\u30a2\u30ea\u30eb\u306e\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u7d76\u5bfe\u5b9a\u91cf\u306f\u91ce\u751f\u578b\u3068\u5909\u7570\u578b\u306e\u767a\u73fe\u306e\u9055\u3044\u3092\u89e3\u660e\u3057\u3001\u307e\u305f\u65b0\u898f\u306e\u30ec\u30a2\u306a\u5909\u7570\u306e\u691c\u51fa\u3082\u53ef\u80fd\u306b\u3059\u308b<\/strong>\u304b\u3089\u3067\u3059\u3002\u7e70\u308a\u8fd4\u3057\u306b\u306a\u308a\u307e\u3059\u304c\u3001\u30c7\u30b8\u30bf\u30ebPCR\u306f\u6a19\u6e96\u66f2\u7dda\u3092\u5fc5\u8981\u3068\u3057\u307e\u305b\u3093\u3002\u3055\u3089\u306b\u690d\u7269\u30b5\u30f3\u30d7\u30eb\u3067\u3088\u304f\u898b\u3089\u308c\u308b\u3055\u307e\u3056\u307e\u306aPCR\u963b\u5bb3\u7269\u8cea\u306b\u5bfe\u3057\u3066\u9ad8\u3044\u8010\u6027\u3092\u3082\u3063\u3066\u3044\u307e\u3059\u3002<\/p>\n

\u9ad8\u7cbe\u5ea6\u306a\u907a\u4f1d\u5b50\u767a\u73fe\u89e3\u6790<\/h2>\n

\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306f\u3001\u4e00\u822c\u7684\u306b\u907a\u4f1d\u5b50\u767a\u73fe\u306e\u89e3\u6790\u306b\u4f7f\u308f\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u901a\u5e38\u306f2\u500d\u4ee5\u4e0a\u306e\u767a\u73fe\u91cf\u306e\u9055\u3044\u304c\u306a\u3044\u3068\u8b58\u5225\u304c\u56f0\u96e3\u3067\u3059<\/strong>\u3002\u907a\u4f1d\u5b50\u767a\u73fe\u306e\u9055\u3044\u3092\u89e3\u6790\u3059\u308b\u306b\u306f\u3001\u4e00\u822c\u7684\u306b\u03b2\u30a2\u30af\u30c1\u30f3\u306a\u3069\u306e\u7d44\u7e54\u306b\u3088\u3063\u3066\u767a\u73fe\u91cf\u306b\u5dee\u306e\u306a\u3044\u3068\u8003\u3048\u3089\u308c\u308b\u30cf\u30a6\u30b9\u30ad\u30fc\u30d4\u30f3\u30b0\u907a\u4f1d\u5b50\u3092\u5185\u90e8\u6a19\u6e96\u3068\u3057\u3066\u5229\u7528\u3057\u307e\u3059\u3002<\/p>\n

\u30c7\u30b8\u30bf\u30ebPCR\u306f\u3001\u00b110%\u4ee5\u4e0b\u306e\u9ad8\u3044\u7cbe\u5ea6\u3067\u306e\u6e2c\u5b9a\u3092\u53ef\u80fd\u3068\u3057\u3001\u907a\u4f1d\u5b50\u767a\u73fe\u306e\u5dee\u7570\u3092\u89e3\u6790<\/a>\u3059\u308b\u5834\u5408\u306b\u304a\u3044\u3066\u30822\u500d\u4ee5\u4e0b\u306e\u308f\u305a\u304b\u306a\u5dee\u7570\u306e\u8b58\u5225\u3092\u53ef\u80fd\u306b\u3057\u307e\u3059<\/strong>\u3002\u307e\u305f\u3001\u5185\u90e8\u6a19\u6e96\u3092\u4f7f\u308f\u306a\u304f\u3066\u3082\u7d76\u5bfe\u5b9a\u91cf\u3092\u884c\u3046\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n

\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3068\u540c\u69d8\u306b\u3001\u30c7\u30b8\u30bf\u30ebPCR\u3067\u3082RNA\u304b\u3089cDNA\u3078\u9006\u8ee2\u5199\u3092\u884c\u3044\u307e\u3059\u3002\u9ad8\u611f\u5ea6\u3067\u691c\u51fa\u3059\u308b\u305f\u3081\u306b\u306f\u3001\u9006\u8ee2\u5199\u52b9\u7387\u304c\u3068\u3066\u3082\u91cd\u8981\u306a\u30d5\u30a1\u30af\u30bf\u30fc\u3067\u3059\u3002\u5f0a\u793e\u3067\u306f\u30c7\u30b8\u30bf\u30ebPCR\u306e\u907a\u4f1d\u5b50\u767a\u73fe\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u3092\u5186\u6ed1\u306b\u9032\u3081\u3066\u3044\u305f\u3060\u304f\u305f\u3081\u306e\u52b9\u7387\u7684\u306a\u9006\u8ee2\u5199\u30ad\u30c3\u30c8\u3068\u3057\u3066\u3001High-Capacity RNA-to-cDNA Kit<\/a>\u3092\u63d0\u4f9b\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n

\"digitalpcr6-fig2\"<\/a>

\u30c7\u30b8\u30bf\u30ebPCR\uff08\u5de6\uff09\u3068\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0 qPCR\uff08\u53f3\uff09\u306e\u5b9a\u91cf\u7cbe\u5ea6\u306e\u6bd4\u8f03\u3002<\/p><\/div>\n

\u8aac\u660e: \u30b5\u30f3\u30d7\u30eb1\u304b\u30895\u306f\u3001\u5272\u5408\u306e\u7570\u306a\u308bmiRNA\u6df7\u5408\u30b5\u30f3\u30d7\u30eb\uff08hsa-miR-19b\u304a\u3088\u3073 hsa-miR-92\uff09\u3067\u3059\u3002\u30b5\u30f3\u30d7\u30eb 1: 100%\u3001\u30b5\u30f3\u30d7\u30eb 2: 95%\u3001\u30b5\u30f3\u30d7\u30eb 3: 90%\u3001\u30b5\u30f3\u30d7\u30eb 4: 75%\u3001\u30b5\u30f3\u30d7\u30eb 5: 50%\u3002\u9006\u8ee2\u5199\u3057\u305fcDNA\u3092\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3001\u304a\u3088\u3073\u30c7\u30b8\u30bf\u30ebPCR \uff08QuantStudio 3D\u30b7\u30b9\u30c6\u30e0\uff09\u3067\u5b9a\u91cf\u3057\u307e\u3057\u305f\u3002\u5404\u56f3\u306e\u53f3\u5074\u306e\u591a\u91cd\u6bd4\u8f03\uff08Tukey-Kramer HSD test\uff09\u304c\u793a\u3059\u3068\u304a\u308a\u3001\u5186\u306e\u91cd\u306a\u308a\u304c\u306a\u3044\u30c7\u30b8\u30bf\u30ebPCR\uff08\u5de6\u56f3\uff09\u306f\u3001\u30b5\u30f3\u30d7\u30eb1\u30682\u306e\u9593\u306e5%\u306e\u9055\u3044\u3092\u8b58\u5225\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u308f\u304b\u308a\u307e\u3059\u3002\u4e00\u65b9\u3067\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0 qPCR\uff08\u53f3\u56f3\uff09\u306f\u30b5\u30f3\u30d7\u30eb1\u30683\u306e\u9593\u306e10%\u306e\u9055\u3044\u3092\u8b58\u5225\u3067\u304d\u307e\u305b\u3093\u3067\u3057\u305f\u3002<\/p>\n

\u30a6\u30a3\u30eb\u30b9\u306e\u7d76\u5bfe\u5b9a\u91cf<\/h2>\n

\u75c5\u539f\u4f53\u30a6\u30a3\u30eb\u30b9\u306e\u529b\u4fa1\u6e2c\u5b9a\u306e\u4ee3\u7528\u3068\u3057\u3066\u3001\u751f\u4f53\u30b5\u30f3\u30d7\u30eb\u4e2d\u306e\u30a6\u30a3\u30eb\u30b9\u30b3\u30d4\u30fc\u6570\u306e\u7d76\u5bfe\u5b9a\u91cf<\/a>\u304c\u884c\u308f\u308c\u3066\u3044\u307e\u3059\u3002\u3057\u304b\u3057\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3092\u7528\u3044\u305f\u30a6\u30a3\u30eb\u30b9\u91cf\u306e\u6e2c\u5b9a\u3067\u306f\u30a2\u30c3\u30bb\u30a4\u306e\u5897\u5e45\u52b9\u7387\u3084\u6a5f\u5668\u306e\u30ad\u30e3\u30ea\u30d6\u30ec\u30fc\u30b7\u30e7\u30f3\u306e\u72b6\u614b\u306e\u5f71\u97ff\u3092\u53d7\u3051\u308b\u3053\u3068\u3084\u3001\u672a\u77e5\u306e\u30b5\u30f3\u30d7\u30eb\u306eCt\u5024\u304b\u3089\u7d76\u5bfe\u5024\u3092\u6c42\u3081\u308b\u306b\u306f\u6a19\u6e96\u30b5\u30f3\u30d7\u30eb\u3068\u306e\u6bd4\u8f03\u304c\u5fc5\u8981\u3001\u3068\u3044\u3063\u305f\u8ab2\u984c\u3092\u30af\u30ea\u30a2\u3059\u308b\u3053\u3068\u304c\u6c42\u3081\u3089\u308c\u307e\u3059<\/strong>\u3002<\/p>\n

\u305d\u308c\u306b\u5bfe\u3057\u3001\u30c7\u30b8\u30bf\u30ebPCR\u306f\u5897\u5e45\u9014\u4e2d\u306e\u5dee\u306f\u7d50\u679c\u306b\u5f71\u97ff\u3057\u307e\u305b\u3093\u3002\u4ee3\u308f\u308a\u306b\u3001\u30c7\u30b8\u30bf\u30ebPCR\u3067\u306f\u3001\u30b5\u30f3\u30d7\u30eb\u3092\u5897\u5e45\u524d\u306b\u4f55\u5343\u3082\u306e\u500b\u3005\u306e\u30a8\u30f3\u30c9\u30dd\u30a4\u30f3\u30c8PCR\u53cd\u5fdc\u30a6\u30a7\u30eb\u306b\u5206\u914d\u3057\u3001\u5bfe\u8c61\u306e\u30a6\u30a3\u30eb\u30b9\u914d\u5217\u306e\u5897\u5e45\u306e\u6709\u7121\u306b\u3088\u3063\u3066\u967d\u6027\u307e\u305f\u306f\u9670\u6027\u304b\u30a6\u30a7\u30eb\u6bce\u306b\u5224\u5b9a\u3057\u307e\u3059\u3002\u9670\u6027\u3068\u306a\u3063\u305f\u30a6\u30a7\u30eb\u306e\u6570\u304b\u3089\u3001\u5143\u306e\u30b5\u30f3\u30d7\u30eb\u4e2d\u306e\u30a6\u30a3\u30eb\u30b9\u6fc3\u5ea6\u3092\u7b97\u51fa\u3057\u307e\u3059<\/strong>\u3002<\/p>\n

\u5404\u53cd\u5fdc\u304c\u967d\u6027\u304b\u9670\u6027\u304b\u306e\u307f\u5224\u5b9a\u3059\u308b\u30c7\u30b8\u30bf\u30ebPCR\u306e\u6e2c\u5b9a\u7cfb\u3067\u306f\u3001\u6e2c\u5b9a\u5024\u304c\u30a2\u30c3\u30bb\u30a4\u306e\u52b9\u7387\u3084\u6a5f\u5668\u306e\u30ad\u30e3\u30ea\u30d6\u30ec\u30fc\u30b7\u30e7\u30f3\u72b6\u614b\u306e\u9055\u3044\u306e\u5f71\u97ff\u3092\u6700\u5c0f\u9650\u306b\u6291\u3048\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u3053\u306e\u3053\u3068\u306b\u3088\u308a\u3001\u5916\u7684\u8981\u56e0\u306e\u5e72\u6e09\u3092\u53d7\u3051\u306a\u3044\u6a19\u6e96\u5316\u3055\u308c\u305f\u30a6\u30a3\u30eb\u30b9\u30b3\u30d4\u30fc\u6570\u306e\u5b9a\u91cf\u7d50\u679c\u304c\u5f97\u3089\u308c\u308b\u306e\u3067\u3001\u305d\u306e\u307e\u307e\u8907\u6570\u306e\u7814\u7a76\u5ba4\u9593\u3067\u30c7\u30fc\u30bf\u3092\u6bd4\u8f03\u3067\u304d\u307e\u3059\u3002<\/p>\n

\u30ea\u30d5\u30a1\u30ec\u30f3\u30b9\u304a\u3088\u3073\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\u306e\u7d76\u5bfe\u5b9a\u91cf<\/h2>\n

\u6b63\u78ba\u306a\u907a\u4f1d\u5b50\u5b9a\u91cf\u306b\u306f\u3001\u591a\u304f\u306e\u5834\u5408\u3001\u30ea\u30d5\u30a1\u30ec\u30f3\u30b9\u30b5\u30f3\u30d7\u30eb\u3084\u30a2\u30c3\u30bb\u30a4\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\u3068\u306e\u6bd4\u8f03\u304c\u5fc5\u8981\u3067\u3059\u3002\u305f\u3060\u5b9f\u969b\u306b\u306f\u3001\u9069\u5207\u306a\u30ea\u30d5\u30a1\u30ec\u30f3\u30b9\u30b5\u30f3\u30d7\u30eb\u304c\u7121\u3044\u30b1\u30fc\u30b9\u304c\u591a\u3005\u3042\u308a\u307e\u3059\uff08\u751f\u7269\u7a2e\u306e\u554f\u984c\u3084\u3001\u7279\u5b9a\u75be\u60a3\u306b\u304a\u3044\u3066\u7279\u7570\u7684\u306a\u907a\u4f1d\u5b50\u306a\u3069\u304c\u8a72\u5f53\u3057\u307e\u3059\uff09\u3002\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3092\u7528\u3044\u3066\u30ea\u30d5\u30a1\u30ec\u30f3\u30b9\u30b5\u30f3\u30d7\u30eb\u3092\u4f5c\u6210\u3059\u308b\u5834\u5408\u3001\u30ea\u30d5\u30a1\u30ec\u30f3\u30b9\u30b5\u30f3\u30d7\u30eb\u306e\u521d\u3081\u306e\u8f03\u6b63\u65b9\u6cd5\u3084\u3001\u9577\u671f\u5b89\u5b9a\u6027\u306e\u78ba\u8a8d\u3001\u304a\u3088\u3073\u5168\u3066\u306e\u7814\u7a76\u3092\u5b8c\u4e86\u3059\u308b\u306e\u306b\u5341\u5206\u306a\u91cf\u304c\u3042\u308b\u304b\u306a\u3069\u306e\u6761\u4ef6\u691c\u8a0e\u304c\u5fc5\u8981<\/strong>\u3067\u3059\u3002\u3055\u3089\u306b\u3001\u5171\u901a\u306e\u6a19\u6e96\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\u3092\u4f7f\u7528\u3057\u3066\u3044\u306a\u3051\u308c\u3070\u3001\u7814\u7a76\u5ba4\u9593\u306e\u30c7\u30fc\u30bf\u6bd4\u8f03\u3082\u305d\u306e\u307e\u307e\u884c\u3046\u3053\u3068\u306b\u306f\u652f\u969c\u304c\u751f\u3058\u307e\u3059\u3002<\/p>\n

\"digitalpcr6-fig3\"<\/a>

\u30c7\u30b8\u30bf\u30eb PCR\u306b\u3088\u308b\u9ad8\u7cbe\u5ea6\u3067\u6b63\u78ba\u306a\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\u306e\u5b9a\u91cf<\/p><\/div>\n

\u8aac\u660e: A: 4\u7a2e\u985e\u306e\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\u30b5\u30f3\u30d7\u30eb\u3092dPCR\u3067\u6e2c\u5b9a\uff08n=2\uff09\u3002\u3070\u3089\u3064\u304d\u304c\u5c11\u306a\u304f\u3001\u7cbe\u5ea6\u304c\u975e\u5e38\u306b\u9ad8\u3044\u5b9a\u91cf\u5024\u304c\u5f97\u3089\u308c\u307e\u3057\u305f\u3002B: \u30b5\u30f3\u30d7\u30eb600-T\u3092\u7528\u3044\u3066\u30014\u6bb5\u968e\u306e10\u500d\u5e0c\u91c8\u7cfb\u5217\u3092\u4f5c\u6210\u3001\u6e2c\u5b9a\u3057\u305f\u7d50\u679c\u3002\u5e0c\u91c8\u500d\u7387\u3068\u5b9a\u91cf\u5024\u306e\u9593\u306b\u306f\u9ad8\u3044\u76f8\u95a2\u6027\uff080.9991\uff09\u304c\u3042\u308a\u3001\u5404\u5e0c\u91c8\u6bb5\u968e\u306b\u304a\u3044\u3066\u3001\u9ad8\u3044\u7cbe\u5ea6\u306e\u5b9a\u91cf\u5024\u3092\u5f97\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3057\u305f\u3002<\/p>\n

\u30c7\u30b8\u30bf\u30ebPCR\u306f\u3001\u76f4\u63a5\u30bf\u30fc\u30b2\u30c3\u30c8\u907a\u4f1d\u5b50\u306e\u30b3\u30d4\u30fc\u6570\u3092\u6c42\u3081\u308b\u7d76\u5bfe\u5b9a\u91cf<\/a>\u3092\u884c\u3046\u3053\u3068\u304c\u3067\u304d\u308b\u306e\u3067\u3001\u65b0\u898f\u306e\u30ea\u30d5\u30a1\u30ec\u30f3\u30b9\u30b5\u30f3\u30d7\u30eb\u3092\u4f5c\u6210\u3059\u308b\u6642\u306e\u8f03\u6b63\u4f5c\u696d\u306b\u6709\u52b9\u6d3b\u7528\u3057\u3066\u3044\u305f\u3060\u304f\u3053\u3068\u304c\u3067\u304d\u307e\u3059<\/strong>\u3002\u30b3\u30d4\u30fc\u6570\u304c\u660e\u78ba\u306a\u30ea\u30d5\u30a1\u30ec\u30f3\u30b9\u30b5\u30f3\u30d7\u30eb\u3092\u5171\u901a\u3067\u7528\u3044\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u76f4\u63a5\u7d50\u679c\u3092\u6bd4\u8f03\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002<\/p>\n

\u307e\u3068\u3081<\/h2>\n

6\u56de\u30b7\u30ea\u30fc\u30ba\u306e\u6700\u7d42\u56de\u3067\u304a\u4f1d\u3048\u3057\u305f\u3044\u3053\u3068\u3001\u305d\u308c\u306f\u3001\u30c7\u30b8\u30bf\u30ebPCR\u306f\u9ad8\u611f\u5ea6\u30fb\u9ad8\u7cbe\u5ea6\u306a\u7d76\u5bfe\u5b9a\u91cf\u3092\u884c\u3046\u3053\u3068\u304c\u3067\u304d\u308b\u306e\u3067\u3001\u69d8\u3005\u306a\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306b\u304a\u3044\u3066\u30a2\u30c3\u30bb\u30a4\u306e\u30d1\u30d5\u30a9\u30fc\u30de\u30f3\u30b9\u306e\u6700\u9069\u5316\u3092\u6709\u52b9\u306b\u884c\u3046\u3053\u3068\u304c\u3067\u304d\u308b<\/strong>\u3001\u3068\u3044\u3046\u3053\u3068\u3067\u3059\u3002\u305f\u3068\u3048\u3070\u3001\u5fae\u91cf\u306e\u75c5\u539f\u83cc\u306e\u691c\u51fa\u5b9a\u91cf\u3084\u3001\u907a\u4f1d\u5b50\u7d44\u307f\u63db\u3048\u4f5c\u7269(GMO)\u691c\u51fa\u3001\u304a\u3088\u3073\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u5b9f\u9a13\u306b\u4f7f\u7528\u3059\u308b\u6a19\u6e96\u30b5\u30f3\u30d7\u30eb\u306e\u7d76\u5bfe\u5b9a\u91cf\u7b49\u306b\u6709\u7528\u3067\u3059<\/strong>\u3002<\/p>\n

\u3010\u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3011\u30c7\u30b8\u30bf\u30ebPCR\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u30ac\u30a4\u30c9<\/h3>\n

\u30c7\u30b8\u30bf\u30ebPCR\u306b\u3088\u308b\u5b9a\u91cf\u304c\u6709\u7528\u306a\u8907\u6570\u306e\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306b\u3064\u3044\u3066\u7d39\u4ecb\u3057\u3066\u3044\u307e\u3059\u3002\u3053\u308c\u304b\u3089\u30c7\u30b8\u30bf\u30ebPCR\u5b9f\u9a13\u3092\u8003\u3048\u3066\u3044\u308b\u65b9\u306b\u6700\u9069\u3067\u3059\u3002<\/p>\n

\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u306f\u3053\u3061\u3089<\/a>
\n <\/p>\n

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\u30c7\u30b8\u30bf\u30ebPCR (dPCR) 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