{"id":385,"date":"2020-04-01T00:00:00","date_gmt":"2020-03-31T15:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/elisa-microplate\/"},"modified":"2025-02-28T17:06:39","modified_gmt":"2025-02-28T08:06:39","slug":"elisa-microplate","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/elisa-microplate\/","title":{"rendered":"\u3053\u308c\u3067\u3082\u3046\u8ff7\u308f\u306a\u3044\uff01ELISA\u7528\u30de\u30a4\u30af\u30ed\u30d7\u30ec\u30fc\u30c8\u306e\u7a2e\u985e\u307e\u3068\u3081"},"content":{"rendered":"

\u306f\u3058\u3081\u306b<\/h2>\n

\u5f0a\u793e\u3067\u306f\u3001\u30b5\u30a4\u30c8\u30ab\u30a4\u30f3ELISA\u30ad\u30c3\u30c8\u306e\u307b\u304b\u3001\u30d7\u30ec\u30fc\u30c8\u3001\u57fa\u8cea\u3001\u6297\u4f53\u3001\u30d0\u30c3\u30d5\u30a1\u30fc\u306a\u3069\u3001\u30de\u30a4\u30af\u30ed\u30d7\u30ec\u30fc\u30c8\u30a2\u30c3\u30bb\u30a4\u7cfb\u306e\u69cb\u7bc9\u306b\u95a2\u3059\u308b\u88fd\u54c1<\/a>\u3092\u5404\u7a2e\u3054\u7528\u610f\u3057\u3066\u3044\u307e\u3059\u3002\u4eca\u56de\u306f\u3001\u30a2\u30c3\u30bb\u30a4\u3067\u8981\u6c42\u3055\u308c\u308b\u611f\u5ea6\u3084\u30c0\u30a4\u30ca\u30df\u30c3\u30af\u30ec\u30f3\u30b8\u306b\u5fdc\u3058\u305fELISA\u7528\u30de\u30a4\u30af\u30ed\u30d7\u30ec\u30fc\u30c8\u306e\u9078\u629e\u306b\u95a2\u3057\u3066<\/strong>\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n

\u30d7\u30ec\u30fc\u30c8\u30d5\u30a9\u30fc\u30de\u30c3\u30c8<\/h2>\n

\u5438\u5149\u6e2c\u5b9a\u3067\u4e00\u822c\u7684\u306b\u7528\u3044\u3089\u308c\u308b\u30af\u30ea\u30a2\u30d7\u30ec\u30fc\u30c8\u306e\u307b\u304b\u306b\u3001\u767d(\u5316\u5b66\u767a\u5149)\u3084\u9ed2(\u86cd\u5149\u3084\u5316\u5b66\u86cd\u5149)\u304c\u3042\u308a\u307e\u3059\u3002\u5316\u5b66\u767a\u5149\u691c\u51fa\u3067\u306f\u30b7\u30b0\u30ca\u30eb\u306e\u5897\u5e45\u3092\u76ee\u7684\u306b\u767d\u8272\u30d7\u30ec\u30fc\u30c8\u3092\u3001\u86cd\u5149\u691c\u51fa\u3067\u306f\u30ce\u30a4\u30ba\u306e\u8efd\u6e1b\u3092\u76ee\u7684\u306b\u9ed2\u8272\u30d7\u30ec\u30fc\u30c8\u3092\u9078\u3076\u3053\u3068\u304c\u591a\u3044<\/strong>\u3067\u3059\u3002\u30d5\u30a9\u30fc\u30de\u30c3\u30c8\u3068\u3057\u3066\u306f96\u7a74\u3068384\u7a74\u304c\u3042\u308a\u307e\u3059\u304c\u300196\u7a74\u30d7\u30ec\u30fc\u30c8\u3067\u4e88\u5099\u5b9f\u9a13\u3057\u305f\u5f8c\u306b384\u7a74\u30d7\u30ec\u30fc\u30c8\u306b\u5909\u66f4\u3057\u3066\u3001\u30b9\u30eb\u30fc\u30d7\u30c3\u30c8\u306e\u5411\u4e0a\u3068\u30b5\u30f3\u30d7\u30eb\u91cf\u3084\u8a66\u85ac\u91cf\u306e\u6e1b\u5c11\u3092\u56f3\u308a\u307e\u3059\u3002<\/p>\n

\u6d3b\u6027\u5316\u306e\u7a2e\u985e<\/h2>\n

\u758e\u6c34\u5438\u7740\u306b\u3088\u308b\u56fa\u76f8\u5316\u306f\u5e83\u304f\u5229\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u758e\u6c34\u5438\u7740\u304c\u96e3\u3057\u3044\u4f4e\u5206\u5b50\u5316\u5408\u7269\u3084\u30da\u30d7\u30c1\u30c9\u3001\u307e\u305f\u306f\u758e\u6c34\u5438\u7740\u3067\u6d3b\u6027\u304c\u4f4e\u4e0b\u3059\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u306f\u3001\u6d3b\u6027\u5316\u30d7\u30ec\u30fc\u30c8<\/a>\u304c\u5229\u7528\u3055\u308c\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n

\u305f\u3068\u3048\u3070\u3001\u758e\u6c34\u5438\u7740\u3067\u306f\u5909\u6027\u3092\u8003\u616e\u3057\u3066\u9ad8\u6fc3\u5ea6\u306e\u6297\u4f53\u3084\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u30a6\u30a7\u30eb\u306b\u6dfb\u52a0(\u901a\u5e38\u3001\u30b5\u30f3\u30c9\u30a4\u30c3\u30c1ELISA\u3067\u306f\u6355\u7372\u6297\u4f53\u30921-9 \u03bcg\/ml\u3067\u30a6\u30a7\u30eb\u306b\u6dfb\u52a0)\u3057\u307e\u3059\u304c\u3001\u758e\u6c34\u5438\u7740\u4ee5\u5916\u306e\u56fa\u76f8\u5316\u6cd5\u3067\u30b3\u30fc\u30c6\u30a3\u30f3\u30b0\u6fc3\u5ea6\u3092\u4e0b\u3052\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n

\u6d3b\u6027\u5316\u30d7\u30ec\u30fc\u30c8\u306b\u306f\u3001\u6297\u30a6\u30b5\u30ae\u3084\u6297\u30de\u30a6\u30b9\u306a\u3069\u306e\u4e8c\u6b21\u6297\u4f53<\/a>\u3001\u30d7\u30ed\u30c6\u30a4\u30f3A, G, A\/G, L\u306a\u3069\u306e\u30a2\u30eb\u30d5\u30a1\u30d9\u30c3\u30c8\u30bf\u30f3\u30d1\u30af\u8cea<\/a>\u3001\u30a2\u30d3\u30b8\u30f3\u3084\u30b9\u30c8\u30ec\u30d7\u30c8\u30a2\u30d3\u30b8\u30f3\u306a\u3069\u306e\u30d3\u30aa\u30c1\u30f3\u7d50\u5408\u6027\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u6d3b\u6027\u5316\u3055\u308c\u305f\u30d7\u30ec\u30fc\u30c8<\/a>\u306e\u307b\u304b\u3001\u30da\u30d7\u30c1\u30c9\u3084\u4f4e\u5206\u5b50\u5316\u5408\u7269\u306e\u30a2\u30df\u30f3\u3084\u30b9\u30eb\u30d5\u30d2\u30c9\u30ea\u30eb\u3068\u81ea\u767a\u7684\u306b\u7d50\u5408\u3059\u308b\u5b98\u80fd\u57fa\u3067\u6d3b\u6027\u5316\u3055\u308c\u305f\u30d7\u30ec\u30fc\u30c8<\/a>\u3082\u3042\u308a\u307e\u3059\u3002<\/p>\n

\u4e8c\u6b21\u6297\u4f53\u3084\u30a2\u30eb\u30d5\u30a1\u30d9\u30c3\u30c8\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u6d3b\u6027\u5316\u3055\u308c\u305f\u30d7\u30ec\u30fc\u30c8\u306f\u6297\u4f53\u56fa\u76f8\u5316\u3092\u76ee\u7684\u3068\u3057\u305f\u88fd\u54c1\u3067\u3059\u304c\u3001\u30b5\u30f3\u30c9\u30a4\u30c3\u30c1ELISA\u306b\u5229\u7528\u3059\u308b\u3068\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9\u30ce\u30a4\u30ba\u304c\u4e0a\u6607\u3059\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u4e8c\u6b21\u6297\u4f53\u30d7\u30ec\u30fc\u30c8\u3067\u306f\u6355\u7372\u30fb\u691c\u51fa\u6297\u4f53\u306e\u6297\u4f53\u7a2e\u304c\u7570\u306a\u308c\u3070\u554f\u984c\u306f\u3042\u308a\u307e\u305b\u3093\u304c\u3001\u30a2\u30eb\u30d5\u30a1\u30d9\u30c3\u30c8\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u306f\u691c\u51fa\u6297\u4f53\u304c\u30a2\u30eb\u30d5\u30a1\u30d9\u30c3\u30c8\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u53cd\u5fdc\u3059\u308b\u305f\u3081\u3001\u30b5\u30f3\u30c9\u30a4\u30c3\u30c1ELISA\u3067\u306e\u5229\u7528\u306f\u96e3\u3057\u304f\u3001\u88fd\u54c1\u306e\u7528\u9014\u306f\u7af6\u5408EIA\u306b\u9650\u3089\u308c\u307e\u3059\u3002<\/p>\n

\u6297\u4f53\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u3001\u30da\u30d7\u30c1\u30c9\u3001\u6838\u9178\u306e\u56fa\u76f8\u5316\u306b\u3001\u6700\u3082\u5229\u7528\u3055\u308c\u308b\u30d7\u30ec\u30fc\u30c8\u306e\u3072\u3068\u3064\u306b\u30d3\u30aa\u30c1\u30f3\u7d50\u5408\u6027\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u6d3b\u6027\u5316\u3055\u308c\u305f\u30d7\u30ec\u30fc\u30c8<\/a>\u304c\u3042\u308a\u307e\u3059\u3002\u5f0a\u793e\u3067\u306f\u3001\u30b9\u30c8\u30ec\u30d7\u30c8\u30a2\u30d3\u30b8\u30f3\u3084\u30cb\u30e5\u30fc\u30c8\u30e9\u30a2\u30d3\u30b8\u30f3\u3067\u6d3b\u6027\u5316\u3057\u305f\u30d7\u30ec\u30fc\u30c8<\/a>\u3092\u5404\u7a2e\u3054\u7528\u610f\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n

\u30d7\u30ec\u30fc\u30c8\u306e\u7a2e\u985e<\/h2>\n

\u5f0a\u793e\u3067\u306f\u3001\u30b9\u30c8\u30ec\u30d7\u30c8\u30a2\u30d3\u30b8\u30f3\u3084\u30cb\u30e5\u30fc\u30c8\u30e9\u30a2\u30d3\u30b8\u30f3\u306b\u95a2\u3057\u3066\u306f\u3001Standard Binding Capacity (SBC)\u3001High Binding Capacity (HBC)\u3001High Sensitivity (HS)\u306e\u30b7\u30ea\u30fc\u30ba\u3092\u3054\u7528\u610f\u3057\u3066\u304a\u308a\u307e\u3059\u3002<\/p>\n

HS\u30d7\u30ec\u30fc\u30c8\u306f\u3001\u72ec\u81ea\u306e\u30c6\u30af\u30ce\u30ed\u30b8\u30fc\u306b\u3088\u308a\u30a6\u30a7\u30eb\u8868\u9762\u304c\u6d3b\u6027\u5316\u3055\u308c\u3066\u304a\u308a\u3001\u4f4e\u6fc3\u5ea6\u30b5\u30f3\u30d7\u30eb\u6e2c\u5b9a\u6642\u306e\u30b7\u30b0\u30ca\u30eb\u30ce\u30a4\u30ba\u6bd4\u306e\u5411\u4e0a\u3092\u76ee\u7684\u3068\u3057\u305f\u88fd\u54c1\u3067\u3059\u3002HS\u30d7\u30ec\u30fc\u30c8\u306e\u6a19\u6e96\u30d7\u30ed\u30c8\u30b3\u30eb\u3067\u306f\u758e\u6c34\u5438\u7740\u6cd5\u306e1\/1,000\u7a0b\u5ea6\u306e\u5e0c\u8584\u306a\u6297\u4f53 (ng\/ml)\u3067\u30b3\u30fc\u30c6\u30a3\u30f3\u30b0\u3092\u884c\u3044\u307e\u3059\u3002<\/p>\n

\"elisa-microplate-fig1\"<\/a>

\u56f3\u3000High Sensitivity Coated Plates\u306b\u3088\u308b\u4f4e\u6fc3\u5ea6\u30d3\u30aa\u30c1\u30f3\u6a19\u8b58\u30b5\u30f3\u30d7\u30eb\u306e\u691c\u51fa\u3002\u30d3\u30aa\u30c1\u30f3\u6a19\u8b58\u30a6\u30b5\u30aeIgG\u3092streptavidin-coated high sensitivity (HS) plates\u307e\u305f\u306fstreptavidin-coated standard binding capacity (SBC) plates\u306b\u6dfb\u52a0\u3057\u307e\u3057\u305f\u300230\u5206\u9593\u5f8c\u3001TBS\u3067\u6d17\u6d44\u3001100 ng\/ml HRP\u6a19\u8b58\u30e4\u30ae\u6297\u30a6\u30b5\u30aeIgG\u306830\u5206\u9593\u53cd\u5fdc\u3055\u305b\u307e\u3057\u305f\u3002\u691c\u51fa\u306fThermo Scientific QuantaBlu Fluorogenic Peroxidase Substrate\u306b\u3088\u308a\u3001430nm\u3067\u86cd\u5149\u6e2c\u5b9a\u3057\u307e\u3057\u305f\u3002<\/p><\/div>\n

\u9ad8\u3044\u30b9\u30eb\u30fc\u30d7\u30c3\u30c8\u304c\u8981\u6c42\u3055\u308c\u308b\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u30a2\u30c3\u30bb\u30a4\u3067\u306f\u7279\u306b\u30c0\u30a4\u30ca\u30df\u30c3\u30af\u30ec\u30f3\u30b8\u3092\u91cd\u8996\u3057\u3066\u30a2\u30c3\u30bb\u30a4\u3092\u30c7\u30b6\u30a4\u30f3\u3059\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u30bf\u30fc\u30b2\u30c3\u30c8\u6fc3\u5ea6\u304c\u672a\u77e5\u306a\u30b5\u30f3\u30d7\u30eb\u3092\u6e2c\u5b9a\u3059\u308b\u5834\u5408\u3001\u30b5\u30f3\u30d7\u30eb\u4e2d\u306e\u30bf\u30fc\u30b2\u30c3\u30c8\u6fc3\u5ea6\u304c\u30c0\u30a4\u30ca\u30df\u30c3\u30af\u306b\u53ce\u307e\u308b\u3088\u3046\u306b\u30b5\u30f3\u30d7\u30eb\u306e\u5e0c\u91c8\u7cfb\u5217(2\u500d, 5\u500d, 10\u500d\u306a\u3069)\u3092\u8abf\u88fd\u3057\u3066\u5225\u3005\u306b\u6e2c\u5b9a\u3057\u307e\u3059\u304c\u3001\u5e0c\u91c8\u7cfb\u5217\u6570\u306e\u5897\u52a0\u306b\u3057\u305f\u304c\u3063\u3066\u6e2c\u5b9a\u6570\u3082\u5897\u3048\u308b\u3053\u3068\u306b\u306a\u308a\u307e\u3059\u3002\u30c0\u30a4\u30ca\u30df\u30c3\u30af\u30ec\u30f3\u30b8\u306e\u5e83\u3044\u30a2\u30c3\u30bb\u30a4\u306f\u5e0c\u91c8\u7cfb\u5217\u6570\u3092\u6e1b\u3089\u3059\u3053\u3068\u304c\u3067\u304d\u308b\u305f\u3081\u3001\u6e2c\u5b9a\u6570\u3092\u6e1b\u3089\u3059\u305f\u3081\u3001\u30c0\u30a4\u30ca\u30df\u30c3\u30af\u30ec\u30f3\u30b8\u306e\u5e83\u3044\u30a2\u30c3\u30bb\u30a4\u30c7\u30b6\u30a4\u30f3\u304c\u597d\u307e\u308c\u307e\u3059\u3002<\/p>\n

\u30c0\u30a4\u30ca\u30df\u30c3\u30af\u30ec\u30f3\u30b8\u3092\u5e83\u3052\u308b\u306b\u306f\u3001\u7d50\u5408\u30ad\u30e3\u30d1\u30b7\u30c6\u30a3\u306e\u9ad8\u3044\u30d7\u30ec\u30fc\u30c8\u3092\u7528\u3044\u308b\u306e\u304c\u4e00\u822c\u7684\u3067\u3059\u3002\u4e0b\u306e\u8868\u3067\u306f\u3001\u5f0a\u793e\u306e\u30b9\u30c8\u30ec\u30d7\u30c8\u30a2\u30d3\u30b8\u30f3\u30d7\u30ec\u30fc\u30c8\u3068\u30cb\u30e5\u30fc\u30c8\u30e9\u30a2\u30d3\u30b8\u30f3\u30d7\u30ec\u30fc\u30c8\u306b\u95a2\u3057\u3066\u3001\u7d50\u5408\u30ad\u30e3\u30d1\u30b7\u30c6\u30a3\u3068\u30c0\u30a4\u30ca\u30df\u30c3\u30af\u30ec\u30f3\u30b8\u306e\u6bd4\u8f03\u3092\u884c\u3063\u3066\u3044\u307e\u3059\u3002<\/p>\n\n\n\n\n\n\n\n
\u8868 \u30d3\u30aa\u30c1\u30f3\u7d50\u5408\u6027\u30bf\u30f3\u30d1\u30af\u8cea\u30d7\u30ec\u30fc\u30c8\u306e\u7279\u6027<\/caption>\n
<\/td>\nHigh Sensitivity (HS)<\/td>\nHigh Binding Capacity (HBC)<\/td>\nStandard Binding Capacity (SBC)<\/td>\n<\/tr>\n
\u7528\u9014<\/td>\n\u9ad8\u611f\u5ea6\u306a\u30a2\u30c3\u30bb\u30a4<\/td>\n\u30c0\u30a4\u30ca\u30df\u30c3\u30af\u30ec\u30f3\u30b8\u306e\u5e83\u3044\u30a2\u30c3\u30bb\u30a4<\/td>\n\u6a19\u6e96\u7684\u306a\u30a2\u30c3\u30bb\u30a4<\/td>\n<\/tr>\n
\u30d3\u30aa\u30c1\u30f3\u6a19\u8b58\u7269\u306e\u63a8\u5968\u30b5\u30a4\u30ba<\/td>\n> 26kDa<\/td>\n> 8kDa<\/td>\n> 8kDa<\/td>\n<\/tr>\n
\u5b9a\u91cf\u57df**
\nStreptavidin Plates<\/td>\n		5 \u301c 300 ng\/mL<\/td>\n62 \u301c 10,000 ng\/mL<\/td>\n31 \u301c 1,250 ng\/mL<\/td>\n<\/tr>\n
\u5b9a\u91cf\u57df **
\nNeutrAvidin Plates<\/td>\n		5 \u301c 125 ng\/mL<\/td>\n15 \u301c 2,500 ng\/mL<\/td>\n15 \u301c 300 ng\/mL<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

** \u30d3\u30aa\u30c1\u30f3\u6a19\u8b58\u30a6\u30b5\u30aeIgG (100 ng\/ml)\u3068HRP\u6a19\u8b58\u30e4\u30ae\u6297\u30a6\u30b5\u30aeIgG (100 ng\/ml)\u304a\u3088\u3073Thermo Scientific QuantaBlu Fluorogenic Peroxidase Substrate\u306b\u3088\u308a\u6c7a\u5b9a\u3057\u305f\u3002<\/span><\/p>\n

\u30b9\u30c8\u30ec\u30d7\u30c8\u30a2\u30d3\u30b8\u30f3\/\u30cb\u30e5\u30fc\u30c8\u30e9\u30a2\u30d3\u30b8\u30f3\u30d7\u30ec\u30fc\u30c8\u3068\u3082\u306b\u3001HBC\u30d7\u30ec\u30fc\u30c8\u3067\u306fSBC\u30d7\u30ec\u30fc\u30c8\u306b\u6bd4\u3079\u3066\u5316\u5b66\u86cd\u5149\u691c\u51fa\u306b\u304a\u3051\u308b\u30c0\u30a4\u30ca\u30df\u30c3\u30af\u30ec\u30f3\u30b8\u304c\u5927\u304d\u304f\u6539\u5584\u3055\u308c\u3066\u3044\u308b\u3053\u3068\u304c\u308f\u304b\u308a\u307e\u3059\u3002<\/p>\n

\u6700\u5f8c\u306b<\/h2>\n

\u30de\u30a4\u30af\u30ed\u30d7\u30ec\u30fc\u30c8\u30a2\u30c3\u30bb\u30a4\u3067\u306f\u3001\u30a2\u30c3\u30bb\u30a4\u3092\u69cb\u6210\u3059\u308b\u8a66\u85ac\u306e\u7d44\u307f\u5408\u308f\u305b\u306b\u3088\u308a\u611f\u5ea6\u3084\u30c0\u30a4\u30ca\u30df\u30c3\u30af\u30ec\u30f3\u30b8\u304c\u5927\u304d\u304f\u5909\u66f4\u3055\u308c\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u5f0a\u793e\u3067\u306f\u3001\u30a2\u30c3\u30bb\u30a4\u7528\u9014\u306b\u5fdc\u3058\u3066\u6700\u9069\u306a\u88fd\u54c1\u3092\u9078\u629e\u3057\u3066\u3044\u305f\u3060\u3051\u308b\u3088\u3046\u3001\u3055\u307e\u3056\u307e\u306a\u88fd\u54c1\u3092\u3054\u7528\u610f\u3057\u3066\u304a\u308a\u307e\u3059\u3002<\/p>\n

\u3010\u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3011\u30bf\u30f3\u30d1\u30af\u8cea\u89e3\u6790\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u30cf\u30f3\u30c9\u30d6\u30c3\u30af<\/h1>\n

\u52b9\u7387\u7684\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u62bd\u51fa\u304b\u3089\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u89e3\u6790\u30c4\u30fc\u30eb\u307e\u3067\u3001\u5305\u62ec\u7684\u306b\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u3092\u7d39\u4ecb\u3057\u3066\u304a\u308a\u307e\u3059\u3002PDF\u30d5\u30a1\u30a4\u30eb\u306e\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3092\u3054\u5e0c\u671b\u306e\u65b9\u306f\u3001\u4e0b\u8a18\u30dc\u30bf\u30f3\u3088\u308a\u304a\u7533\u8fbc\u307f\u304f\u3060\u3055\u3044\u3002<\/p>\n

\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/p>\n

 <\/p>\n

\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u7528\u306b\u306f\u4f7f\u7528\u3044\u305f\u3060\u3051\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

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