{"id":417,"date":"2016-06-06T00:00:00","date_gmt":"2016-06-06T00:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/method-of-affinity-purification\/"},"modified":"2025-03-10T10:33:33","modified_gmt":"2025-03-10T01:33:33","slug":"method-of-affinity-purification","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/method-of-affinity-purification\/","title":{"rendered":"\u30a2\u30d5\u30a3\u30cb\u30c6\u30a3\u7cbe\u88fd\u306e\u62c5\u4f53\u3084\u7cbe\u88fd\u65b9\u6cd5\u307e\u3068\u3081"},"content":{"rendered":"<h2>\u306f\u3058\u3081\u306b<\/h2>\n<p>\u6297\u4f53\u7cbe\u88fd\u3084(\u5171)\u514d\u75ab\u6c88\u964d\u306a\u3069\u306e\u30a2\u30d5\u30a3\u30cb\u30c6\u30a3\u7cbe\u88fd\u3067\u306f\u30a2\u30ac\u30ed\u30fc\u30b9\u306a\u3069\u306e\u591a\u5b54\u6027\u30ec\u30b8\u30f3\u3092\u7528\u3044\u305f\u30d0\u30c3\u30c1\u6cd5\u3084\u30ec\u30b8\u30f3\u5145\u586b\u30ab\u30e9\u30e0\u306b\u3088\u308b\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u3001\u78c1\u6027\u7c92\u5b50\u306b\u3088\u308b\u78c1\u6027\u5206\u96e2\u304c\u5229\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u4eca\u56de\u306f\u3001\u30a2\u30d5\u30a3\u30cb\u30c6\u30a3\u7cbe\u88fd\u306e\u62c5\u4f53\u3084\u624b\u6cd5\u306e\u9078\u629e\u306b\u3064\u3044\u3066\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n<h2>\u30d0\u30c3\u30c1\u6cd5\u3068\u30b9\u30d4\u30f3\u30ab\u30e9\u30e0\u6cd5<\/h2>\n<p>\u5f93\u6765\u306e\u514d\u75ab\u6c88\u964d\u3067\u306f\u7d30\u80de\u62bd\u51fa\u6db2\u306a\u3069\u306e\u30b5\u30f3\u30d7\u30eb\u306b\u5bfe\u3057\u3066\u3001\u6297\u539f\u7279\u7570\u6297\u4f53\u3092\u6dfb\u52a0\u3057\u3066\u6297\u539f\u6297\u4f53\u8907\u5408\u4f53\u3092\u5f62\u6210\u3055\u305b\u305f\u5f8c\u3001\u30d7\u30ed\u30c6\u30a4\u30f3A\u3084\u30d7\u30ed\u30c6\u30a4\u30f3G\u3092\u56fa\u5b9a\u5316\u3057\u305f\u30a2\u30ac\u30ed\u30fc\u30b9\u3092\u6dfb\u52a0\u3001\u6d17\u6d44\u3084\u6eb6\u51fa\u306f\u9060\u5fc3\u5f8c\u30da\u30ec\u30c3\u30c8\u306b\u5bfe\u3057\u3066\u884c\u3044\u307e\u3059\u3002<\/p>\n<p>\u3053\u306e\u65b9\u6cd5\u306f\u30d0\u30c3\u30c1\u6cd5\u3068\u3082\u547c\u3070\u308c\u3001\u6d17\u6d44\u3084\u6eb6\u51fa\u306e\u969b\u306b\u6c88\u964d\u30da\u30ec\u30c3\u30c8\u304b\u3089\u4e0a\u6e05\u3092\u5206\u96e2\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u304c\u3001\u591a\u5b54\u6027\u30ec\u30b8\u30f3\u3092\u30a2\u30d5\u30a3\u30cb\u30c6\u30a3\u62c5\u4f53\u306b\u7528\u3044\u305f(\u5171)\u514d\u75ab\u6c88\u964d\u3067\u306f\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5438\u7740\u306b\u3088\u308b\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9\u30ce\u30a4\u30ba\u306e\u4e0a\u6607\u304c\u307f\u3089\u308c\u308b\u3053\u3068\u304c\u3042\u308a\u3001\u7279\u306b\u4f4e\u6fc3\u5ea6\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u30b5\u30f3\u30d7\u30eb\u304b\u3089\u30a2\u30d5\u30a3\u30cb\u30c6\u30a3\u7cbe\u88fd\u3092\u884c\u3046\u5834\u5408\u3001\u63a5\u89e6\u52b9\u7387\u304c\u9ad8\u304f\u30ec\u30b8\u30f3\u6dfb\u52a0\u91cf\u3092\u6700\u5c0f\u5316\u3067\u304d\u308b\u30d0\u30c3\u30c1\u6cd5\u304c\u9078\u629e\u3055\u308c\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u30d0\u30c3\u30c1\u6cd5\u306f\u53cd\u5fdc\u30b9\u30b1\u30fc\u30eb\u306e\u5909\u66f4\u3082\u5bb9\u6613\u3067\u73fe\u5728\u3067\u3082\u4e00\u822c\u7684\u306b\u5229\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u30da\u30ec\u30c3\u30c8\u306e\u5438\u5f15\u3084\u4e0a\u6e05\u306e\u56de\u53ce\u7387\u304c\u554f\u984c\u306b\u306a\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3057\u305f\u3002<\/p>\n<p>\u3053\u306e\u3088\u3046\u306a\u554f\u984c\u3092\u89e3\u6c7a\u3059\u308b\u305f\u3081\u5f0a\u793e\u306e(\u5171)\u514d\u75ab\u6c88\u964d\u30ad\u30c3\u30c8\u3067\u306f<strong>\u6297\u539f\u6297\u4f53\u53cd\u5fdc\u5f8c\u306b\u30ec\u30b8\u30f3\u3092\u30b9\u30d4\u30f3\u30ab\u30e9\u30e0\u306b\u79fb\u3057\u3066\u6d17\u6d44\u3084\u6eb6\u51fa\u3092\u884c\u3046\u30d7\u30ed\u30c8\u30b3\u30eb\u3084\u3001\u30ec\u30b8\u30f3\u5145\u586b\u30ab\u30e9\u30e0\u306b\u6297\u4f53\u3092\u56fa\u5b9a\u5316\u3057\u3066\u7528\u3044\u308b\u30d7\u30ed\u30c8\u30b3\u30eb\u304c\u63a1\u7528\u3055\u308c\u3066\u3044\u307e\u3059<\/strong>\u3002\u307e\u305f\u3001\u78c1\u6027\u7c92\u5b50\u3092\u7528\u3044\u305f\u30d0\u30c3\u30c1\u6cd5\u3082(\u5171)\u514d\u75ab\u6c88\u964d\u3067\u7a4d\u6975\u7684\u306b\u5229\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n<h2>\u591a\u5b54\u6027\u30ec\u30b8\u30f3\u3068\u78c1\u6027\u7c92\u5b50<\/h2>\n<p>4%\u307e\u305f\u306f6%\u67b6\u6a4b\u30a2\u30ac\u30ed\u30fc\u30b9\u306f\u591a\u5b54\u6027\u306e\u89aa\u6c34\u6027\u30ec\u30b8\u30f3\u3067\u3001(\u5171)\u514d\u75ab\u6c88\u964d\u3067\u3082\u4e00\u822c\u7684\u306b\u4f7f\u7528\u3055\u308c\u308b\u62c5\u4f53\u3067\u3059\u3002\u9178\u6eb6\u51fa\u306b\u304a\u3051\u308b\u30ea\u30ac\u30f3\u30c9\u306e\u6ef2\u51fa\u3082\u5c11\u306a\u304f\u3001\u3055\u3089\u306b\u6297\u4f53\u3092\u67b6\u6a4b\u3059\u308b\u3053\u3068\u3067\u6297\u4f53\u30ec\u30b8\u30f3\u306f\u901a\u5e388-10\u56de\u7a0b\u5ea6\u306e\u518d\u5229\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href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/wp-content\/uploads\/2016\/05\/method-of-affinity-purification-doc1.pdf?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa3_Social_LAB\n\" target=\"_blank\" rel=\"noopener noreferrer\">Pierce Spin Cup and Columns dimensions and volumes<\/a>(PDF)\u3082\u4f75\u305b\u3066\u3054\u78ba\u8a8d\u304f\u3060\u3055\u3044\u3002<\/p>\n<p>\u78c1\u6027\u7c92\u5b50\u306f\u7c92\u5b50\u5f84\u304c\u5c0f\u3055\u3044(1-2 um)\u305f\u3081\u5358\u4f4d\u91cd\u91cf\u3042\u305f\u308a\u306e\u8868\u9762\u7a4d\u304c\u9ad8\u304f\u3001\u975e\u7279\u7570\u7684\u5438\u7740\u306e\u6700\u5c0f\u5316\u3092\u671f\u5f85\u3057\u3066\u62c5\u4f53\u306b\u4f7f\u7528\u3055\u308c\u307e\u3059\u3002\u3055\u3089\u306b\u3001<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/dna-rna-purification-analysis\/automated-purification-extraction\/kingfisher-systems.html?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa3_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Thermo Scientific KingFisher\u30b7\u30ea\u30fc\u30ba\u7b49\u306e\u78c1\u6027\u7c92\u5b50\u30d7\u30ed\u30bb\u30c3\u30b5<\/a>\u3067\u81ea\u52d5\u5316\u306b\u3082\u5bfe\u5fdc\u3059\u308b\u305f\u3081\u3001\u30c7\u30a3\u30fc\u30d7\u30a6\u30a7\u30eb\u30d7\u30ec\u30fc\u30c8\u3092\u7528\u3044\u305f\u30cf\u30a4\u30b9\u30eb\u30fc\u30d7\u30c3\u30c8\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u3067\u306f\u6700\u9069\u306a\u62c5\u4f53\u3068\u3044\u3048\u307e\u3059\u3002<\/p>\n<p>\u307e\u305f\u5f0a\u793e\u3067\u306f\u3001\u51dd\u96c6\u3092\u6291\u3048\u305f\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u51e6\u7406\u6e08\u307f\u306e\u5b89\u5b9a\u306a<a href=\"https:\/\/www.thermofisher.com\/search\/results?query=Pierce+Magnetic+Beads&amp;navId=0&amp;sort=&amp;persona=Catalog&amp;resultPage=1?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa3_Social_LAB\n\" target=\"_blank\" rel=\"noopener noreferrer\">\u8d85\u5e38\u78c1\u6027\u7c92\u5b50Pierce Magnetic 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