{"id":431,"date":"2020-04-01T00:00:00","date_gmt":"2020-03-31T15:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/westernblot-membrane-stripping\/"},"modified":"2025-01-21T15:51:38","modified_gmt":"2025-01-21T06:51:38","slug":"westernblot-membrane-stripping","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/westernblot-membrane-stripping\/","title":{"rendered":"\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306b\u5931\u6557\u3057\u305f\uff01\u3068\u304d\u306b\u7c21\u5358\u306b\u3084\u308a\u76f4\u3059\u65b9\u6cd5"},"content":{"rendered":"<h2>\u6297\u4f53\u3092\u5265\u304c\u3059\u306b\u306f\u30b9\u30c8\u30ea\u30c3\u30d4\u30f3\u30b0\uff01<\/h2>\n<p>\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306f\u3001\u96fb\u6c17\u6cf3\u52d5\u304b\u3089\u59cb\u307e\u3063\u3066\u30e1\u30f3\u30d6\u30ec\u30f3\u3078\u306e\u30c8\u30e9\u30f3\u30b9\u30d5\u30a1\u30fc\u3001\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u3001\u4e00\u6b21\u30fb\u4e8c\u6b21\u6297\u4f53\u53cd\u5fdc\u3092\u7d4c\u3066\u7d50\u679c\u304c\u5f97\u3089\u308c\u307e\u3059\u3002\uff11\u3064\u306e\u30b5\u30f3\u30d7\u30eb\u306b\u5bfe\u3057\u3066\u8907\u6570\u306e\u4e00\u6b21\u6297\u4f53\u3092\u4f7f\u3044\u305f\u3044\u5834\u5408\u3001\u305d\u306e\u7a2e\u985e\u306b\u5fdc\u3058\u305f\u30e1\u30f3\u30d6\u30ec\u30f3\u3092\u6e96\u5099\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u3057\u304b\u3057\u30b5\u30f3\u30d7\u30eb\u91cf\u304c\u9650\u3089\u308c\u3066\u3044\u308b\u3001\u96fb\u6c17\u6cf3\u52d5\uff5e\u30c8\u30e9\u30f3\u30b9\u30d5\u30a1\u30fc\u306e\u624b\u9593\u3084\u6642\u9593\u304c\u304b\u304b\u308b\u3001\u3068\u3044\u3046\u3068\u3053\u308d\u3067\u60a9\u3080\u3053\u3068\u304c\u3042\u308b\u3068\u601d\u3044\u307e\u3059\u3002\u3042\u308b\u3044\u306f\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u691c\u51fa\u304c\u3046\u307e\u304f\u3044\u304b\u305a\u3001\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u3084\u6297\u4f53\u306e\u5e0c\u91c8\u7387\u3092\u691c\u8a0e\u3057\u305f\u3044\u5834\u5408\u3082\u3042\u308b\u304b\u3082\u3057\u308c\u307e\u305b\u3093\u3002<\/p>\n<p>\u305d\u3093\u306a\u3068\u304d\u306b\u4fbf\u5229\u306a\u6280\u8853\u304c\u3001<strong>\u30bf\u30fc\u30b2\u30c3\u30c8\u5206\u5b50\u306e\u691c\u51fa\u3092\u7d42\u3048\u305f\u30e1\u30f3\u30d6\u30ec\u30f3\u304b\u3089\u6297\u4f53\uff08\u4e00\u6b21\u6297\u4f53\u30fb\u4e8c\u6b21\u6297\u4f53\uff09\u3092\u5265\u304c\u3059\u30b9\u30c8\u30ea\u30c3\u30d4\u30f3\u30b0<\/strong>\u3067\u3059\u3002\u30b9\u30c8\u30ea\u30c3\u30d4\u30f3\u30b0\u3092\u884c\u3046\u3053\u3068\u3067\u3001<strong>\u8ee2\u5199\u6e08\u307f\u306e\u540c\u3058\u30e1\u30f3\u30d6\u30ec\u30f3\u4e0a\u3067\u518d\u3073\u6297\u4f53\u53cd\u5fdc\u3092\u884c\u3046\u3053\u3068\u304c\u3067\u304d\u3001\u96fb\u6c17\u6cf3\u52d5\u3068\u8ee2\u5199\u306e\u624b\u9593\u3092\u7701\u304f\u3053\u3068\u304c\u3067\u304d\u307e\u3059<\/strong>\u3002<\/p>\n<p>\u5f93\u6765\u306e\u30b9\u30c8\u30ea\u30c3\u30d4\u30f3\u30b0\u30d0\u30c3\u30d5\u30a1\u30fc\u306f\u30e1\u30eb\u30ab\u30d7\u30c8\u30a8\u30bf\u30ce\u30fc\u30eb\u3084\u5909\u6027\u5264\u3092\u542b\u307f\u3001\u6297\u4f53\u3092\u5265\u96e2\u3059\u308b\u969b\u306b\u30e1\u30f3\u30d6\u30ec\u30f3\u4e0a\u306e\u6297\u539f\u306b\u30c0\u30e1\u30fc\u30b8\u3092\u4e0e\u3048\u308b\u305f\u3081\u3001\u518d\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u3092\u5fc5\u8981\u3068\u3057\u305f\u308a\u3001\u518d\u30d7\u30ed\u30fc\u30d6\u3067\u6297\u4f53\u6fc3\u5ea6\u3092\u9ad8\u3081\u305f\u308a\u3001\u30d5\u30a3\u30eb\u30e0\u3078\u306e\u611f\u5149\u6642\u9593\u3092\u5ef6\u9577\u3059\u308b\u5fc5\u8981\u304c\u3042\u308b\u3068\u3055\u308c\u307e\u3059\u3002\u5834\u5408\u306b\u3088\u3063\u3066\u306f\u3001\u6297\u4f53\u306e\u53cd\u5fdc\u52b9\u7387\u304c\u8457\u3057\u304f\u4f4e\u4e0b\u3059\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<p>\u5f0a\u793e\u88fd\u54c1\u3067\u3042\u308b<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/21063?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa4_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Restore Western Blot Stripping Buffer<\/a>\u306f\u3001\u30a6\u30a7\u30b9\u30bf\u30f3\u3092\u7c21\u5358\u306b\u3084\u308a\u76f4\u3059\u3053\u3068\u304c\u3067\u304d\u308b\u8a66\u85ac\u3068\u3057\u3066\u3054\u597d\u8a55\u3092\u3044\u305f\u3060\u3044\u3066\u304a\u308a\u307e\u3059\u3002<strong>\u3053\u3061\u3089\u306e\u88fd\u54c1\u306f\u30e1\u30eb\u30ab\u30d7\u30bf\u30f3\u3092\u542b\u307e\u306a\u3044\u30de\u30a4\u30eb\u30c9\u306a\u7d44\u6210\u3067\u6297\u4f53\u3092\u5265\u96e2\u3059\u308b\u305f\u3081\u3001\u6297\u539f\u3092\u50b7\u3081\u308b\u3053\u3068\u3082\u3042\u308a\u307e\u305b\u3093<\/strong>\u3002\u4e00\u6b21\u6297\u4f53\u306e\u30a2\u30d5\u30a3\u30cb\u30c6\u30a3\u306e\u5f37\u3055\u306b\u3082\u3088\u308a\u307e\u3059\u304c\u30015-15\u5206\u9593\u3067\u30b9\u30c8\u30ea\u30c3\u30d7\u304c\u5b8c\u4e86\u3057\u307e\u3059\u3002\u307e\u305f\u3001\u305d\u306e\u6b21\u306e\u6297\u4f53\u3067\u691c\u51fa\u306e\u969b\u306f\u901a\u5e38\u3001\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u3092\u3084\u308a\u76f4\u3059\u5fc5\u8981\u306f\u3042\u308a\u307e\u305b\u3093\u3002<strong><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/21063?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa4_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Restore Western Blot Stripping Buffer<\/a>\u3067\u306f\u5265\u304c\u308c\u306a\u3044\u3088\u3046\u306a\u9ad8\u30a2\u30d5\u30a3\u30cb\u30c6\u30a3\u306e\u6297\u4f53\u306b\u5bfe\u3057\u3066\u306f\u3001<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/46428?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa4_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Restore Plus Western Blot Stripping Buffer<\/a>\u304c\u3042\u308a\u307e\u3059\u3002\u3053\u3061\u3089\u306e\u88fd\u54c1\u3067\u306f\u30b9\u30c8\u30ea\u30c3\u30d4\u30f3\u30b0\u5f8c\u306e\u518d\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u304c\u5fc5\u8981\u3067\u3059<\/strong>\u3002<\/p>\n<h3>Restore Western Blot Stripping Buffer\u306e\u7279\u9577<\/h3>\n<ul>\n<li>\u52b9\u7387\u7684\u306a\u6297\u4f53\u9664\u53bb\uff1a\u72ec\u81ea\u958b\u767a\u3057\u305f\u30d0\u30c3\u30d5\u30a1\u30fc\u304c\u52b9\u679c\u7684\u306b\u4f5c\u7528\u3057\u3001\u6297\u4f53\u3092\u9664\u53bb\u3057\u307e\u3059\u3002\u901a\u5e38\u30b9\u30c8\u30ea\u30c3\u30d4\u30f3\u30b0\u306f\u7d0410\u5206\u3067\u5b8c\u4e86\u3057\u307e\u3059\u3002<\/li>\n<li>\u7a4f\u548c\u306a\u8a66\u85ac\uff1a\u6297\u4f53\u9664\u53bb\u306e\u969b\u306b\u6297\u539f\u3078\u306e\u30c0\u30e1\u30fc\u30b8\u304c\u5c11\u306a\u304f\u3001\u6b21\u306e\u6297\u4f53\u53cd\u5fdc\u3078\u306e\u5f71\u97ff\u306f\u307b\u3068\u3093\u3069\u3042\u308a\u307e\u305b\u3093\u3002<\/li>\n<li>\u52b4\u529b\u306e\u7bc0\u7d04\uff1a\u8ee2\u5199\u6e08\u307f\u306e\u30e1\u30f3\u30d6\u30ec\u30f3\u3092\u518d\u5229\u7528\u3059\u308b\u305f\u3081\u3001\u96fb\u6c17\u6cf3\u52d5\u30fb\u8ee2\u5199\u306e\u624b\u9593\u3092\u7701\u304f\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/li>\n<li>\u30b5\u30f3\u30d7\u30eb\u306e\u7bc0\u7d04\uff1a\u96fb\u6c17\u6cf3\u52d5\u306e\u56de\u6570\u304c\u5c11\u306a\u304f\u306a\u308b\u5206\u3001\u30b5\u30f3\u30d7\u30eb\u3082\u7bc0\u7d04\u3067\u304d\u307e\u3059\u3002<\/li>\n<\/ul>\n<h1>\u3010\u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3011\u30bf\u30f3\u30d1\u30af\u8cea\u89e3\u6790\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u30cf\u30f3\u30c9\u30d6\u30c3\u30af<\/h1>\n<p>\u52b9\u7387\u7684\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u62bd\u51fa\u304b\u3089\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u89e3\u6790\u30c4\u30fc\u30eb\u307e\u3067\u3001\u5305\u62ec\u7684\u306b\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u3092\u7d39\u4ecb\u3057\u3066\u304a\u308a\u307e\u3059\u3002PDF\u30d5\u30a1\u30a4\u30eb\u306e\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3092\u3054\u5e0c\u671b\u306e\u65b9\u306f\u3001\u4e0b\u8a18\u30dc\u30bf\u30f3\u3088\u308a\u304a\u7533\u8fbc\u307f\u304f\u3060\u3055\u3044\u3002<\/p>\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/catalogs-protein-form.html?CID=bid_pca_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pa4_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/p>\n<p>&nbsp;<\/p>\n<p>\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u7528\u306b\u306f\u4f7f\u7528\u3044\u305f\u3060\u3051\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306b\u5931\u6557\u3057\u305f\uff01\u3042\u308b\u3044\u306f\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u691c\u51fa\u304c\u3046\u307e\u304f\u3044\u304b\u305a\u3001\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u3084\u6297\u4f53\u306e\u5e0c\u91c8\u7387\u3092\u691c\u8a0e\u3057\u305f\u3044\u5834\u5408\u306b\u4fbf\u5229\u306a\u6280\u8853\u304c\u30b9\u30c8\u30ea\u30c3\u30d4\u30f3\u30b0\u3067\u3059\u3002\u4eca\u56de\u306f\u3001\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306b\u304a\u3051\u308b\u30b9\u30c8\u30ea\u30c3\u30d4\u30f3\u30b0\u306b\u3064\u3044\u3066\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n","protected":false},"author":988,"featured_media":2699,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[156],"tags":[17],"division":[],"class_list":{"0":"post-431","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-mol-bio","8":"tag-western-blotting","9":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"<!-- 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